Spg001732 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001732
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionglycine-rich cell wall structural protein 1.8-like
Locationscaffold8: 33668063 .. 33668299 (-)
RNA-Seq ExpressionSpg001732
SyntenySpg001732
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTGTAATGAAAGTTTACTTGGAATTGTTGAAGCAAGGCCTCTCCATATGGACAGCGCAGTAGCTACAAATGATTTCTTGGCGAGATTATCTCTTGGAGCCATCAAGCAGTCTGGTCCAAGCCCCGGCGGCGATGGCCACAAGTTCACTGATGTCGATACTCTTGGTGGTATCAAGGACTCCGGCCTGAGCCCTGGCGTCGGCCACAGCCTAATCACCGACCAACGCCCTTGA

mRNA sequence

ATGATGTGTAATGAAAGTTTACTTGGAATTGTTGAAGCAAGGCCTCTCCATATGGACAGCGCAGTAGCTACAAATGATTTCTTGGCGAGATTATCTCTTGGAGCCATCAAGCAGTCTGGTCCAAGCCCCGGCGGCGATGGCCACAAGTTCACTGATGTCGATACTCTTGGTGGTATCAAGGACTCCGGCCTGAGCCCTGGCGTCGGCCACAGCCTAATCACCGACCAACGCCCTTGA

Coding sequence (CDS)

ATGATGTGTAATGAAAGTTTACTTGGAATTGTTGAAGCAAGGCCTCTCCATATGGACAGCGCAGTAGCTACAAATGATTTCTTGGCGAGATTATCTCTTGGAGCCATCAAGCAGTCTGGTCCAAGCCCCGGCGGCGATGGCCACAAGTTCACTGATGTCGATACTCTTGGTGGTATCAAGGACTCCGGCCTGAGCCCTGGCGTCGGCCACAGCCTAATCACCGACCAACGCCCTTGA

Protein sequence

MMCNESLLGIVEARPLHMDSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLGGIKDSGLSPGVGHSLITDQRP
Homology
BLAST of Spg001732 vs. NCBI nr
Match: KAG6595008.1 (PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027036.1 hypothetical protein SDJN02_11045, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 95.9 bits (237), Expect = 1.6e-16
Identity = 49/68 (72.06%), Postives = 52/68 (76.47%), Query Frame = 0

Query: 12 EARPLH-MDSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLGGIKDSGLSPGVGH 71
          EARPL+ M SA    DF   LSLGAIKQSGPSPGGDGHKF + DTLGGIK+SG SPG GH
Sbjct: 28 EARPLNAMASAAIAEDFFEGLSLGAIKQSGPSPGGDGHKFVNFDTLGGIKESGPSPGDGH 87

Query: 72 SLITDQRP 79
          S IT  RP
Sbjct: 88 SHITSSRP 95

BLAST of Spg001732 vs. NCBI nr
Match: KAE8647505.1 (hypothetical protein Csa_003084 [Cucumis sativus])

HSP 1 Score: 84.7 bits (208), Expect = 3.8e-13
Identity = 44/80 (55.00%), Postives = 53/80 (66.25%), Query Frame = 0

Query: 1   MMCNESLLGIVEARPLHM------DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVD 60
           ++ N  L+ + +ARPL +       +A    DF   LSLGAIKQSGPS GGDGHKF + D
Sbjct: 23  LLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNYD 82

Query: 61  TLGGIKDSGLSPGVGHSLIT 75
           TLGGIKDSG +PG GH  IT
Sbjct: 83  TLGGIKDSGPAPGDGHRHIT 102

BLAST of Spg001732 vs. NCBI nr
Match: TYK12857.1 (hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa])

HSP 1 Score: 84.7 bits (208), Expect = 3.8e-13
Identity = 43/78 (55.13%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHM----DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTL 60
          ++ N  L+ + EARPL +     +A    DF   LSLGAIKQSGPS GGDGHKF + DT 
Sbjct: 14 LLDNGGLVIVTEARPLGLMGSGSAAAIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNYDTF 73

Query: 61 GGIKDSGLSPGVGHSLIT 75
          GG+KDSG +PG GHS +T
Sbjct: 74 GGMKDSGPTPGDGHSHVT 91

BLAST of Spg001732 vs. NCBI nr
Match: KAF7828678.1 (PAMP-induced secreted peptide 2-like [Senna tora])

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-11
Identity = 42/76 (55.26%), Postives = 54/76 (71.05%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHMDSAVATN-----DFLARLSLGAIKQSGPSPGGDGHKFTDVDT 60
          ++ N + + ++EARPL +   V++      DF   LSLGAIKQSGPS GG+GHKFT+ +T
Sbjct: 6  LLVNTAFVVVLEARPLSIIKEVSSGTRDEVDFFDWLSLGAIKQSGPSGGGEGHKFTNTET 65

Query: 61 LGGIKDSGLSP-GVGH 71
          LGGIKDSG SP G GH
Sbjct: 66 LGGIKDSGPSPSGPGH 81

BLAST of Spg001732 vs. NCBI nr
Match: KAG6571202.1 (hypothetical protein SDJN03_30117, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.0 bits (188), Expect = 7.9e-11
Identity = 39/66 (59.09%), Postives = 45/66 (68.18%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHMDSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLGGIK 60
          ++ NES  G V+AR LHM +   T +FL  LS GAIK  G S  GDGH FTDV TLGGIK
Sbjct: 12 LLSNESCFGTVQARHLHMTNFTTTRNFLGGLSFGAIKNFGSSCSGDGHNFTDVVTLGGIK 71

Query: 61 DSGLSP 67
          +SG SP
Sbjct: 72 NSGPSP 77

BLAST of Spg001732 vs. ExPASy Swiss-Prot
Match: F4JRC5 (PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.6e-04
Identity = 33/67 (49.25%), Postives = 40/67 (59.70%), Query Frame = 0

Query: 10 IVEARPLHMDSAVATNDFLAR----LSLGAIKQSGPSPGGDGHKFTD-VDTLGGIKDSGL 69
          +VE+RPL +        F+A     LSLG+IK SGPSP G+GHK  D  DT   +K SG 
Sbjct: 21 LVESRPLGLTK--TEEKFVASLFDGLSLGSIKDSGPSP-GEGHKVVDRKDTFRFVKHSGP 80

Query: 70 SP-GVGH 71
          SP G GH
Sbjct: 81 SPSGPGH 84

BLAST of Spg001732 vs. ExPASy TrEMBL
Match: A0A5D3CLN9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004490 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-13
Identity = 43/78 (55.13%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHM----DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTL 60
          ++ N  L+ + EARPL +     +A    DF   LSLGAIKQSGPS GGDGHKF + DT 
Sbjct: 14 LLDNGGLVIVTEARPLGLMGSGSAAAIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNYDTF 73

Query: 61 GGIKDSGLSPGVGHSLIT 75
          GG+KDSG +PG GHS +T
Sbjct: 74 GGMKDSGPTPGDGHSHVT 91

BLAST of Spg001732 vs. ExPASy TrEMBL
Match: A0A0A0KIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-13
Identity = 44/80 (55.00%), Postives = 53/80 (66.25%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHM------DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVD 60
          ++ N  L+ + +ARPL +       +A    DF   LSLGAIKQSGPS GGDGHKF + D
Sbjct: 17 LLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNYD 76

Query: 61 TLGGIKDSGLSPGVGHSLIT 75
          TLGGIKDSG +PG GH  IT
Sbjct: 77 TLGGIKDSGPAPGDGHRHIT 96

BLAST of Spg001732 vs. ExPASy TrEMBL
Match: A0A059AZU8 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.2e-10
Identity = 40/68 (58.82%), Postives = 47/68 (69.12%), Query Frame = 0

Query: 12 EARPLHM-----DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLGGIKDSGLSP 71
          EARP ++        VA   F   LSLGAIKQ+GPSP G+GHKFT+ +TLGGIKDSG SP
Sbjct: 25 EARPFNVMETKAPVGVACGGFFDGLSLGAIKQAGPSP-GEGHKFTNSETLGGIKDSGPSP 84

Query: 72 GVGHSLIT 75
          G GH  +T
Sbjct: 85 GEGHGHVT 91

BLAST of Spg001732 vs. ExPASy TrEMBL
Match: V7CCF8 (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_003G239800g PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-09
Identity = 41/80 (51.25%), Postives = 52/80 (65.00%), Query Frame = 0

Query: 1  MMCNESLLGIVEARPLHMD---SAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLG 60
          ++ +  L+G+ EARPL +      V   DF   LSLGAIK+SGPSPGG GH+FT+ +TLG
Sbjct: 16 LVMSTGLMGL-EARPLSISGTGEGVEVVDFFDWLSLGAIKESGPSPGGKGHRFTNSNTLG 75

Query: 61 GIKDSG-LSPGVGHSLITDQ 77
          GIKDSG  S G GH     +
Sbjct: 76 GIKDSGPSSGGEGHKFTNSE 94

BLAST of Spg001732 vs. ExPASy TrEMBL
Match: A0A059B1H0 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02237 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.1e-09
Identity = 39/64 (60.94%), Postives = 45/64 (70.31%), Query Frame = 0

Query: 12 EARPLHM-----DSAVATNDFLARLSLGAIKQSGPSPGGDGHKFTDVDTLGGIKDSGLSP 71
          EARP ++        VA   F   LSLGAIKQ+GPSP G+GHKFT+ +TLGGIKDSG SP
Sbjct: 25 EARPFNVMETKAPVGVACGGFFDGLSLGAIKQAGPSP-GEGHKFTNSETLGGIKDSGPSP 84

BLAST of Spg001732 vs. TAIR 10
Match: AT2G23270.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: stem, sperm cell, root, stamen; EXPRESSED DURING: 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G37290.1); Has 36 Blast hits to 35 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 36; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 1.5e-07
Identity = 39/86 (45.35%), Postives = 47/86 (54.65%), Query Frame = 0

Query: 1  MMCNESLLG------------IVEARPLHMDSAV--ATNDFLARLSLGAIKQSGPSPGGD 60
          MM N+ +LG            +VEARPL +  A       F   LSLGAIK+SGPS GG+
Sbjct: 1  MMMNKVVLGSFLFFMLVSSNFVVEARPLGLTKAEENLVAKFFDGLSLGAIKESGPSSGGE 60

Query: 61 GHKFTD-VDTLGGIKDSGLS-PGVGH 71
          GH+F D  +TL   K SG S  G GH
Sbjct: 61 GHRFVDRTETLEYGKHSGPSTSGPGH 86

BLAST of Spg001732 vs. TAIR 10
Match: AT4G37290.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: endomembrane system; EXPRESSED IN: cotyledon, hypocotyl, leaf; EXPRESSED DURING: LP.04 four leaves visible; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G23270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 45.8 bits (107), Expect = 1.8e-05
Identity = 33/67 (49.25%), Postives = 40/67 (59.70%), Query Frame = 0

Query: 10 IVEARPLHMDSAVATNDFLAR----LSLGAIKQSGPSPGGDGHKFTD-VDTLGGIKDSGL 69
          +VE+RPL +        F+A     LSLG+IK SGPSP G+GHK  D  DT   +K SG 
Sbjct: 21 LVESRPLGLTK--TEEKFVASLFDGLSLGSIKDSGPSP-GEGHKVVDRKDTFRFVKHSGP 80

Query: 70 SP-GVGH 71
          SP G GH
Sbjct: 81 SPSGPGH 84

BLAST of Spg001732 vs. TAIR 10
Match: AT1G49800.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: sepal, male gametophyte, stamen; EXPRESSED DURING: 4 anthesis; Has 12 Blast hits to 12 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 12; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 20/38 (52.63%), Postives = 22/38 (57.89%), Query Frame = 0

Query: 33  LGAIKQSGPSPGGDGHKFTDVDTLGGIKDSGLSPGVGH 71
           L  +KQSGPSPG   H+       G   DSG SPGVGH
Sbjct: 71  LATVKQSGPSPGVGHHRAKGYKMFGRANDSGPSPGVGH 108

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6595008.11.6e-1672.06PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia]... [more]
KAE8647505.13.8e-1355.00hypothetical protein Csa_003084 [Cucumis sativus][more]
TYK12857.13.8e-1355.13hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa][more]
KAF7828678.13.5e-1155.26PAMP-induced secreted peptide 2-like [Senna tora][more]
KAG6571202.17.9e-1159.09hypothetical protein SDJN03_30117, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
F4JRC52.6e-0449.25PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A5D3CLN91.8e-1355.13Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0KIE21.8e-1355.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1[more]
A0A059AZU84.2e-1058.82Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV... [more]
V7CCF81.2e-0951.25Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_003G239800g PE=4 ... [more]
A0A059B1H02.1e-0960.94Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02237 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G23270.11.5e-0745.35unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT4G37290.11.8e-0549.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response... [more]
AT1G49800.12.0e-0452.63unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044700PIP2/PIPL1PANTHERPTHR34663OS06G0637400 PROTEINcoord: 4..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001732.1Spg001732.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045087 innate immune response
biological_process GO:0050793 regulation of developmental process
molecular_function GO:0016740 transferase activity