Spg001480 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001480 vs. NCBI nr
Match: KAG6604975.1 (Transport and Golgi organization protein 2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 2.0e-131
Identity = 240/302 (79.47%), Postives = 248/302 (82.12%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 56  MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 115

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 116 RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 175

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+ TPLDFAKEV KEAHQYNGFNLIIADI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 176 -----SKTTPLDFAKEVEKEAHQYNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVPPG 235

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLMTNT+KDD SLLPGIY
Sbjct: 236 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPGIY 295

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISALSVKNSGEASFFE+HLENDAWID+SFTFQI+
Sbjct: 296 SPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQIE 321

Query: 301 NF 303
           NF
Sbjct: 356 NF 321

BLAST of Spg001480 vs. NCBI nr
Match: XP_022971076.1 (transport and Golgi organization protein 2 homolog [Cucurbita maxima] >XP_022971077.1 transport and Golgi organization protein 2 homolog [Cucurbita maxima])

HSP 1 Score: 477.6 bits (1228), Expect = 7.8e-131
Identity = 239/302 (79.14%), Postives = 248/302 (82.12%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+KTPLDFAKEV KEAHQ+NGFNLIIADI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 121 -----SKKTPLDFAKEVEKEAHQFNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVPPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLMTNT+KDD SLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISALSVKNSGEASFFE+HLENDAWID+S TFQI+
Sbjct: 241 SPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSVTFQIE 266

Query: 301 NF 303
           NF
Sbjct: 301 NF 266

BLAST of Spg001480 vs. NCBI nr
Match: XP_022947387.1 (transport and Golgi organization protein 2 homolog [Cucurbita moschata] >XP_022947388.1 transport and Golgi organization protein 2 homolog [Cucurbita moschata])

HSP 1 Score: 475.7 bits (1223), Expect = 2.9e-130
Identity = 238/302 (78.81%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+ TPLDFAKEV KEAHQYNGFNLII DI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 121 -----SKTTPLDFAKEVEKEAHQYNGFNLIIVDIPSKTMFYITNRPINNSDSLVTEVPPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLM NT+KDD SLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMINTIKDDVSLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISALSVKNSGEASFFE+HLENDAWID+SFTFQI+
Sbjct: 241 SPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQIE 266

Query: 301 NF 303
           NF
Sbjct: 301 NF 266

BLAST of Spg001480 vs. NCBI nr
Match: XP_022140668.1 (transport and Golgi organization 2 homolog [Momordica charantia])

HSP 1 Score: 471.5 bits (1212), Expect = 5.6e-129
Identity = 233/301 (77.41%), Postives = 251/301 (83.39%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEYR+RPTKPLEWWECG+ILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYRTRPTKPLEWWECGDILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAF+TN REVQSLP AKSRGELPV FLK                               
Sbjct: 61  RVAFVTNFREVQSLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+K PLDFAKEVAKEA QYNGFNLI+A+ISSKTMFYITNRP N+S SLVTEVSPG
Sbjct: 121 -----SKKAPLDFAKEVAKEADQYNGFNLIVAEISSKTMFYITNRPTNDSPSLVTEVSPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKAQRLDQSFR++V+KN DRELP + MVEKLMTNT+KDDESLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKAQRLDQSFRDLVDKNGDRELPTEEMVEKLMTNTIKDDESLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTDTELGRYGTGSISALSVKNSGEA+F +R+L+ND WIDNSFTFQI+
Sbjct: 241 SPRREYQYSSIFVDTDTELGRYGTGSISALSVKNSGEATFSQRNLQNDEWIDNSFTFQIE 265

Query: 301 N 302
           N
Sbjct: 301 N 265

BLAST of Spg001480 vs. NCBI nr
Match: XP_023532494.1 (transport and Golgi organization protein 2 homolog [Cucurbita pepo subsp. pepo] >XP_023532495.1 transport and Golgi organization protein 2 homolog [Cucurbita pepo subsp. pepo] >XP_023532496.1 transport and Golgi organization protein 2 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.7 bits (1210), Expect = 9.5e-129
Identity = 235/301 (78.07%), Postives = 245/301 (81.40%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHP+YPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPVYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+ TPLDFAKEV KEAHQYNGFNLIIADI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 121 -----SKTTPLDFAKEVEKEAHQYNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVPPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLMTNT+KDD SLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISAL VKNSGE+SFFE+HLENDAWID+SFTF I+
Sbjct: 241 SPRREYQYSSIFVDTDSELGRYGTGSISALYVKNSGESSFFEKHLENDAWIDSSFTFHIE 265

Query: 301 N 302
           N
Sbjct: 301 N 265

BLAST of Spg001480 vs. ExPASy Swiss-Prot
Match: Q29RZ5 (Transport and Golgi organization protein 2 homolog OS=Bos taurus OX=9913 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.0e-25
Identity = 96/319 (30.09%), Postives = 143/319 (44.83%), Query Frame = 0

Query: 1   MCIAVFLWQAHPL----YPFFLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P+    Y   L  NRDE+  RP +  ++W    E+L G D   G  GGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYHRPARAADFWGSNNEVLSGLDMEEGKEGGT 60

Query: 61  WLACSRNGRVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANK 120
           WL  S  G++A LTN  + +    A+ RGEL   FL                        
Sbjct: 61  WLGISTRGKLAALTNYLQPRLNRNARGRGELVAQFL------------------------ 120

Query: 121 KGTASFVILRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSK---TMFYITNRPINN 180
                        S+   L + K+V+ E H YNGFNLI AD+S++    + Y  NR    
Sbjct: 121 ------------TSDMDSLSYLKKVSAEGHLYNGFNLIAADLSAEKGDVICYYGNR---- 180

Query: 181 SDSLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTV 240
            +     ++PG + L+NA L++PW K     Q F E V +   RELP  A+V +L+   +
Sbjct: 181 GEREPVVLAPGTYGLSNALLETPWRKLCFGKQLFLEAVERG--RELPRDALVAQLLA-VL 240

Query: 241 KDDESLL--PGIYSPRREY--PFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHL 300
            +DE+ L  P I +  REY  P  S +         YGT + + + V   G  +F ER +
Sbjct: 241 SNDEAQLPDPAIEAQGREYVRPILSKYAAVCVRCPDYGTRTSTVILVDADGHVTFTERSM 276

Query: 301 ---ENDAWIDNSFTFQIKN 302
              +   W   +  F++++
Sbjct: 301 LGSDPTRWETTTHEFRLQS 276

BLAST of Spg001480 vs. ExPASy Swiss-Prot
Match: P54797 (Transport and Golgi organization 2 homolog OS=Mus musculus OX=10090 GN=Tango2 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.8e-25
Identity = 94/319 (29.47%), Postives = 147/319 (46.08%), Query Frame = 0

Query: 1   MCIAVFLWQAHPL----YPFFLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P+    Y   L  NRDE+ +RP+K  ++W    EIL G D   G AGGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYNRPSKLADFWGNNSEILSGLDMEEGKAGGT 60

Query: 61  WLACSRNGRVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANK 120
           WL  S  G++  LTN  + +  P A+ RGEL   FL                        
Sbjct: 61  WLGISTRGKLGALTNYLQPRQEPDARGRGELVSHFL------------------------ 120

Query: 121 KGTASFVILRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSK---TMFYITNRPINN 180
                        S+   L + K+V+ E H YNGFN+I AD+S+     + Y  NR    
Sbjct: 121 ------------TSDMDSLSYLKKVSTEGHLYNGFNIIAADLSTSKGDVVCYYGNR--GE 180

Query: 181 SDSLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTV 240
            + +V  ++PG + L+NA L++PW K     Q F E V ++    LP   +V +L+ + +
Sbjct: 181 PEPIV--LTPGTYGLSNALLETPWKKLCFGKQLFMEAVEQS--EALPKDVLVTQLL-DVL 240

Query: 241 KDDESLL--PGIYSPRREY--PFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHL 300
            ++E+ L  P I    +EY  P  + +         YGT + + + V  +G  +F ER +
Sbjct: 241 NNEEAQLPDPAIEDQGQEYVQPILNKYAAVWCRCASYGTRTNTIILVDANGHVTFTERSM 276

Query: 301 ---ENDAWIDNSFTFQIKN 302
              +   W  N++ F +++
Sbjct: 301 LDKDTSRWETNTYEFTLQS 276

BLAST of Spg001480 vs. ExPASy Swiss-Prot
Match: Q6ICL3 (Transport and Golgi organization protein 2 homolog OS=Homo sapiens OX=9606 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.3e-23
Identity = 95/319 (29.78%), Postives = 143/319 (44.83%), Query Frame = 0

Query: 1   MCIAVFLWQAHPL----YPFFLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P+    Y   L  NRDE+ SRP+K  ++W    EIL G D   G  GGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYSRPSKLADFWGNNNEILSGLDMEEGKEGGT 60

Query: 61  WLACSRNGRVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANK 120
           WL  S  G++A LTN  + Q    A+ RGEL   FL                        
Sbjct: 61  WLGISTRGKLAALTNYLQPQLDWQARGRGELVTHFL------------------------ 120

Query: 121 KGTASFVILRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISS---KTMFYITNRPINN 180
                        ++   L + K+V+ E H YNGFNLI AD+S+     + Y  NR    
Sbjct: 121 ------------TTDVDSLSYLKKVSMEGHLYNGFNLIAADLSTAKGDVICYYGNR--GE 180

Query: 181 SDSLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTV 240
            D +V  ++PG + L+NA L++PW K     Q F E V ++  + LP   ++  L+ + +
Sbjct: 181 PDPIV--LTPGTYGLSNALLETPWRKLCFGKQLFLEAVERS--QALPKDVLIASLL-DVL 240

Query: 241 KDDESLL--PGIYSPRREY--PFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHL 300
            ++E+ L  P I     EY  P  S +         YGT + + + V   G  +F ER +
Sbjct: 241 NNEEAQLPDPAIEDQGGEYVQPMLSKYAAVCVRCPGYGTRTNTIILVDADGHVTFTERSM 276

Query: 301 ---ENDAWIDNSFTFQIKN 302
              +   W   ++ F +++
Sbjct: 301 MDKDLSHWETRTYEFTLQS 276

BLAST of Spg001480 vs. ExPASy TrEMBL
Match: A0A6J1I2B1 (transport and Golgi organization protein 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469854 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.8e-131
Identity = 239/302 (79.14%), Postives = 248/302 (82.12%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+KTPLDFAKEV KEAHQ+NGFNLIIADI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 121 -----SKKTPLDFAKEVEKEAHQFNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVPPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLMTNT+KDD SLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISALSVKNSGEASFFE+HLENDAWID+S TFQI+
Sbjct: 241 SPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSVTFQIE 266

Query: 301 NF 303
           NF
Sbjct: 301 NF 266

BLAST of Spg001480 vs. ExPASy TrEMBL
Match: A0A6J1G6B2 (transport and Golgi organization protein 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC111451268 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.4e-130
Identity = 238/302 (78.81%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRGELPV FLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+ TPLDFAKEV KEAHQYNGFNLII DI SKTMFYITNRPINNSDSLVTEV PG
Sbjct: 121 -----SKTTPLDFAKEVEKEAHQYNGFNLIIVDIPSKTMFYITNRPINNSDSLVTEVPPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKA RLDQS RE VNKN DRELPVK MVEKLM NT+KDD SLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMINTIKDDVSLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTD+ELGRYGTGSISALSVKNSGEASFFE+HLENDAWID+SFTFQI+
Sbjct: 241 SPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQIE 266

Query: 301 NF 303
           NF
Sbjct: 301 NF 266

BLAST of Spg001480 vs. ExPASy TrEMBL
Match: A0A6J1CII2 (transport and Golgi organization 2 homolog OS=Momordica charantia OX=3673 GN=LOC111011269 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.7e-129
Identity = 233/301 (77.41%), Postives = 251/301 (83.39%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLWQAHPLYPFFLLLNRDEYR+RPTKPLEWWECG+ILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYRTRPTKPLEWWECGDILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAF+TN REVQSLP AKSRGELPV FLK                               
Sbjct: 61  RVAFVTNFREVQSLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+K PLDFAKEVAKEA QYNGFNLI+A+ISSKTMFYITNRP N+S SLVTEVSPG
Sbjct: 121 -----SKKAPLDFAKEVAKEADQYNGFNLIVAEISSKTMFYITNRPTNDSPSLVTEVSPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNAQLDSPWPKAQRLDQSFR++V+KN DRELP + MVEKLMTNT+KDDESLLPGIY
Sbjct: 181 IHVLTNAQLDSPWPKAQRLDQSFRDLVDKNGDRELPTEEMVEKLMTNTIKDDESLLPGIY 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
           SPRREY +SSIFVDTDTELGRYGTGSISALSVKNSGEA+F +R+L+ND WIDNSFTFQI+
Sbjct: 241 SPRREYQYSSIFVDTDTELGRYGTGSISALSVKNSGEATFSQRNLQNDEWIDNSFTFQIE 265

Query: 301 N 302
           N
Sbjct: 301 N 265

BLAST of Spg001480 vs. ExPASy TrEMBL
Match: A0A2N9FED1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13380 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.5e-98
Identity = 188/300 (62.67%), Postives = 215/300 (71.67%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAVFLWQAHPLYP  LLLNRDEY SRPT+ LEWWE  EILGGRD LAGGTWLACSR+G
Sbjct: 66  MCIAVFLWQAHPLYPLILLLNRDEYHSRPTRQLEWWEGDEILGGRDELAGGTWLACSRDG 125

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTNVREVQSLP AKSRG+LPV FL+                               
Sbjct: 126 RVAFLTNVREVQSLPEAKSRGDLPVRFLQ------------------------------- 185

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+K P++FA+EVAKE  QYNGFNLI+ D+  KTM Y+TNR     +  VTEVS G
Sbjct: 186 -----SKKKPMEFAEEVAKEMDQYNGFNLILIDLCCKTMVYVTNR-AKEDNKFVTEVSTG 245

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVLTNA LDSPWPKAQRL  SF+E++ K  + ELP+K MVEKLMTNTVKDDES+LP I+
Sbjct: 246 IHVLTNASLDSPWPKAQRLGNSFKELLEKYGEEELPMKEMVEKLMTNTVKDDESMLPHIF 305

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
            P REY  SSIFVDT++ LG YGT S SAL VK +GE  F+ERHLEN+ W + + T+QIK
Sbjct: 306 PPEREYHLSSIFVDTNSPLGPYGTRSTSALFVKATGEVGFYERHLENELWKEQAVTYQIK 328

BLAST of Spg001480 vs. ExPASy TrEMBL
Match: A0A6A1UGL1 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR0G025184 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 5.1e-96
Identity = 178/302 (58.94%), Postives = 216/302 (71.52%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAVFLWQAHPLYP  LL NRDEY SRPT+PL WWE G++L GRD LAGGTWLACSR+G
Sbjct: 1   MCIAVFLWQAHPLYPLILLQNRDEYHSRPTRPLAWWEGGQVLAGRDELAGGTWLACSRDG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           RVAFLTN REV+ LP AKSRG+LPV FL+                               
Sbjct: 61  RVAFLTNFREVKKLPEAKSRGDLPVRFLQ------------------------------- 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
                S+K P +FA+EV KEA QYNGFNLI+ DI SKTM Y+TNR     D  VT+V PG
Sbjct: 121 -----SKKKPAEFAEEVVKEADQYNGFNLILTDIGSKTMVYVTNR-AKEGDCSVTKVEPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
            HVLTNA L+SPWPKA+RL  +F+E+++KN ++E+P+K MVEKLM NTVKDDES+LP I+
Sbjct: 181 THVLTNATLNSPWPKAERLGTNFKELLSKNGEKEVPMKEMVEKLMMNTVKDDESMLPHIH 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLENDAWIDNSFTFQIK 300
            P  EYP SS+FVD+D+ LGRYGT SISAL VK +G   F+ERHLEN+ W + + T+QI+
Sbjct: 241 PPEWEYPLSSVFVDSDSPLGRYGTRSISALFVKTTGGVDFYERHLENELWKEQTLTYQIE 265

Query: 301 NF 303
           ++
Sbjct: 301 DY 265

BLAST of Spg001480 vs. TAIR 10
Match: AT4G38260.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 305.4 bits (781), Expect = 4.9e-83
Identity = 157/306 (51.31%), Postives = 199/306 (65.03%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAVFLWQ+HPLYPF L LNRDE  +R T+ L WWE GE +GGRD + GGTWL C+R+G
Sbjct: 1   MCIAVFLWQSHPLYPFLLFLNRDEDHNRATEALRWWEDGETVGGRDLVGGGTWLGCTRHG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVI 120
           R+AFLTN +E  S P AKSRG+LP                                    
Sbjct: 61  RLAFLTNFKEASSFPAAKSRGDLP------------------------------------ 120

Query: 121 LRCILSEKTPLDFAKEVAKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPG 180
           LR + SEK+P +FA+E+  E   YNGFNL++A + SK+M YITNRP  + D LVT+VSPG
Sbjct: 121 LRYLQSEKSPAEFAEEIQDEISLYNGFNLVVAHVLSKSMIYITNRP-PHGDKLVTQVSPG 180

Query: 181 IHVLTNAQLDSPWPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIY 240
           IHVL+NA LDSPWPK  RL + F++++ +N   E PVK MVE++MTNTVKD+E+ LP ++
Sbjct: 181 IHVLSNANLDSPWPKCLRLREGFQQLLAENGSGEFPVKTMVEEVMTNTVKDEETELPHVF 240

Query: 241 SPRREYPFSSIFVDTDTELGRYGTGSISALSVKN------SGEASFFERHL-ENDAWIDN 300
           +P  EY  SSIFVD     GRYGT SISA+ VK+       GE  F+ERHL E D+W ++
Sbjct: 241 TPETEYHLSSIFVDMQRPTGRYGTRSISAIIVKSHGDGGGDGEICFYERHLEEGDSWKEH 269

BLAST of Spg001480 vs. TAIR 10
Match: AT1G20730.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 105.1 bits (261), Expect = 9.7e-23
Identity = 92/290 (31.72%), Postives = 137/290 (47.24%), Query Frame = 0

Query: 18  LLLNRDEYRSRPTKPLEW-WECGEILGGRDGLAGGTWLACSRNGRVAFLTNVREVQSLPL 77
           LL NR+ ++SR      W W+  ++L GR     GTWL  S  GRVAFL     V++ P+
Sbjct: 17  LLQNRENWQSRSIGKAAWEWDESQVLSGRCAETDGTWLGISIRGRVAFL-----VEAGPV 76

Query: 78  AKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVILRCILSEKTPLDFAKE 137
            + R         +   C +L       L++NL                   +P DFA  
Sbjct: 77  NRDR--------IIGAECRTLDF-----LKSNL-------------------SPEDFADS 136

Query: 138 VAKEAHQYNG--FNLIIADISSKTMFYITNRPINNSDSLVTE-VSPGIHVLTNAQLDSP- 197
           +A ++ +  G  ++LI+ADI S +MFYI    ++    + TE V PG+H L++A LDS  
Sbjct: 137 LALDSGRNTGIAYHLIVADIVSNSMFYIYKPSLSEDGMVYTEPVGPGVHTLSSAGLDSEV 196

Query: 198 WPKAQRLDQSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIYSPRREYPFSSIF 257
             +  RL + F E +N+      P+  + E++M +TV+                P SSIF
Sbjct: 197 GHRDLRLKRYFSERINRELPE--PISGLAEEVMYDTVEAING-----------DPLSSIF 256

Query: 258 -VDTDTELGRYGTGSISALSVKNSGEASFFERHLE--NDAWIDNSFTFQI 300
            VDT  E   YGT   +AL V+ + +  FFER+    ND W  + F F I
Sbjct: 257 VVDTLIENEHYGTRCTTALVVRRTMQVRFFERYRARFNDDWNVHDFRFTI 256

BLAST of Spg001480 vs. TAIR 10
Match: AT1G20740.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 98.6 bits (244), Expect = 9.1e-21
Identity = 91/281 (32.38%), Postives = 129/281 (45.91%), Query Frame = 0

Query: 28  RPTKPLEW--WECGEILGGRDGLAGGTWLACSRNGRVAFLTNVREVQSLPLAKSRGELPV 87
           R  +  EW   E  +IL GR     GTWL  S  GRVAFL                    
Sbjct: 35  RSIRKAEWVKTETDQILSGRCPETDGTWLGISTRGRVAFL-------------------- 94

Query: 88  GFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVILRCILSEKTPLDFAKEVAKEAHQYN 147
                      ++AG   R + N A  +       L  + S ++P DFAK  A +  +  
Sbjct: 95  -----------VEAGTINRDRFNGAESR------TLEFLESNESPEDFAKSSAADYIRNK 154

Query: 148 ---GFNLIIADISSKTMFYITNRPINNSDSLVTE-VSPGIHVLTNAQLDSP-WPKAQRLD 207
               F+LI+ADI+S +M YI+    ++   + TE V PG+H L++A LDS    +  R+ 
Sbjct: 155 NTAAFHLIVADIASNSMLYISKPRFSDYGIVYTEPVGPGVHTLSSAGLDSDVGYRDLRMR 214

Query: 208 QSFREIVNKNSDRELPVKAMVEKLMTNTVKDDESLLPGIYSPRREYPFSSIFVDTDTELG 267
            SF E++N+  +R  P++ + E +M + VK  ES+L            S  FVD      
Sbjct: 215 HSFCEMINR--ERLPPIRDIAE-IMYDPVKAYESVLLS----------SIFFVDMKIGYE 265

Query: 268 RYGTGSISALSVKNSGEASFFERHLE--NDAWIDNSFTFQI 300
            YGT   +AL VK + E  FFER+ E  ND W D+ F F I
Sbjct: 275 HYGTRITTALVVKRTKEVLFFERYREIFNDDWDDHDFAFTI 265

BLAST of Spg001480 vs. TAIR 10
Match: AT1G20680.2 (Protein of unknown function (DUF833) )

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-20
Identity = 83/308 (26.95%), Postives = 135/308 (43.83%), Query Frame = 0

Query: 1   MCIAVFLWQAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           M I  F W         LL+NRD + +R      W   G+IL GR     GTW   ++ G
Sbjct: 1   MGIVAFEWAEGKTNQLTLLMNRDNWENRVISGASWSRNGQILSGRCKANNGTWFGITKGG 60

Query: 61  RVAFLTNVREVQSLPLAKSRGEL-PVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFV 120
           RVAFL N   +     + S  EL PV FL+                              
Sbjct: 61  RVAFLVNTSLLLDRVKSYSGSELYPVRFLE------------------------------ 120

Query: 121 ILRCILSEKTPLDFAKEV---AKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTE 180
                    +P  FA EV    KE ++ + ++L++AD++S +M +I       SD ++  
Sbjct: 121 ------GNMSPEQFANEVKVHEKETNERHAYSLVVADMTSSSMVHILKPSDTKSDVVIET 180

Query: 181 VSPGIHVLTNAQ-LDS-PWPKAQRLDQSFREIV-NKNSDRELPVKAMVEKLMTNTVKDDE 240
           V  G+H L++ + LDS    +   L + F ++V N  + ++  ++ +  + M +     +
Sbjct: 181 VPFGVHTLSSYEGLDSTDSARDLLLRRLFTQMVGNLGNVQQRQMEEIAGRFMYDAQAGRD 240

Query: 241 SLLPGIYSPRREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLE-NDAWID 300
           ++    Y  R E+P   +         R+GT S +AL VK + E   FE+++E N AW  
Sbjct: 241 AV---FYHSRDEHPNGKLGTQ------RFGTTSTTALVVKRTREVMLFEKYMEQNGAWNT 263

BLAST of Spg001480 vs. TAIR 10
Match: AT1G20680.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 79.7 bits (195), Expect = 4.4e-15
Identity = 79/299 (26.42%), Postives = 131/299 (43.81%), Query Frame = 0

Query: 9   QAHPLYPFFLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNGRVAFLTNV 68
           QA   + F++ L  D    R      W   G+IL GR     GTW   ++ GRVAFL N 
Sbjct: 21  QASLTFSFYVPLVID----RVISGASWSRNGQILSGRCKANNGTWFGITKGGRVAFLVNT 80

Query: 69  REVQSLPLAKSRGELPVGFLKVRYSCTSLQAGPCVRLQTNLANKKGTASFVILRCILSEK 128
               SL L + +           YS + L  G                            
Sbjct: 81  ----SLLLDRVKS----------YSGSELYPG--------------------------NM 140

Query: 129 TPLDFAKEV---AKEAHQYNGFNLIIADISSKTMFYITNRPINNSDSLVTEVSPGIHVLT 188
           +P  FA EV    KE ++ + ++L++AD++S +M +I       SD ++  V  G+H L+
Sbjct: 141 SPEQFANEVKVHEKETNERHAYSLVVADMTSSSMVHILKPSDTKSDVVIETVPFGVHTLS 200

Query: 189 NAQ-LDS-PWPKAQRLDQSFREIV-NKNSDRELPVKAMVEKLMTNTVKDDESLLPGIYSP 248
           + + LDS    +   L + F ++V N  + ++  ++ +  + M +     +++    Y  
Sbjct: 201 SYEGLDSTDSARDLLLRRLFTQMVGNLGNVQQRQMEEIAGRFMYDAQAGRDAV---FYHS 260

Query: 249 RREYPFSSIFVDTDTELGRYGTGSISALSVKNSGEASFFERHLE-NDAWIDNSFTFQIK 301
           R E+P   +         R+GT S +AL VK + E   FE+++E N AW  N+F F I+
Sbjct: 261 RDEHPNGKLGTQ------RFGTTSTTALVVKRTREVMLFEKYMEQNGAWNTNNFAFNIQ 266