Spg001440 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001440
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionreceptor-like cytosolic serine/threonine-protein kinase RBK1
Locationscaffold8: 39475950 .. 39478144 (+)
RNA-Seq ExpressionSpg001440
SyntenySpg001440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTCACTCATCAGGCCCAAGACTCAGAAGAAGAAAACAATTATCTTTGGTCTAAAATCTGACAATGGAAGCAGAGATATTCTTCTCTGGTTGCTTACGACAATCGTCAATCCAGGAGATTACGTTCTTGCTGTTCATGTTCAAGAGCCAAATGATAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAGCAGGTATTTATCACCATTATCAGTCGAGTTGGATTATAGGAGCTAATATGCTGGCCTTCTCTAAAACTAAGGTTGGGATTCAATTGATTTAGTCTTTTGATTTCTGCAGGTTGACTTACAGGTTAAGGTATGCAAAGCTAGTTCTTATATTTCGAACTTAACTCATCAAGTACGAGAGAGCTATGCGACTATTTTGGTACTTGGATGTAGCCTTTCAAGGTATTAGATGAAATAAATGAAACCTCCCTTCCCCAAACTCCTTCTTTCTCTTAGTGTCTTATAAGGTGATTTGTTGCAGACCTAATTACTCAGTTTTCAATACCTGCTTGAAAGGCTTGCCTCCCACTTGCACTCTCATGATGATGGATAATCTAGGAAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAGCGGTTTTACAACCTTCTTGTTCATCAATTTCCAAGTATGCTATAGGATATCAACAAACCAATTCCTGTCACTTACAGAAGTTTTTGACCCTACCCTCTTCTCTACCTAATGAAAAACAAGAAATCTCCCTTGCCAACAAGAAAGCGCGGCCATTTCCCAACTTTGCACCTCGTGAACTGTTTCCTAGATCAGCACTGCAAGATTCAAAAGAAATCAAAAGGCAGTTTACATCCGAACAGCTCAACGGTGCAACCAACAATTTTGATCCTGCAATGGTGATTGGTGAAGGTGGACATAGTAAGGTGTATAGAGCTAACCTTGAAGATGGGCAAGCAGCAGCAGTGAAAGTTCTAAGCTCAGATTCTTCATCCGAAGATCTTGTTCAGGAAGTCGAAATCTTGTCTAAGATAAAACATGAGAACATAGTTCATATTATTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTCAAAGGAAGCCTAAAGCAATTCCTGAGAAAACTGGAGTGGAGGGAGAGAGTGAGGATTGCAATTGGAATAGCAAAAGCACTGAACTATCTTCATCATTCTTGCAACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAAGTAAGTCAATCACATGTTAAACTTTCAAGTGTTAAACAATCTACATTTACAGTTTTGCTCCTAAAAATCACAACTTAAATTTGGTTTCTCCACTCATAGTTAGCAGATTTTGGAGAAGCAACAGTGCTCAAACAGTCTCAAAATGCACAATCCAACACAAAGCCATACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATGATGTATGGAAAGATTGATGAGAAAATAGATGTCTACTCCTATGGAGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCAAACAGATCAGAACAATCGTGCAAGTTTGGTACTCTGGGTAATAACAAAACCAACAATTTCTATTACAAATTACTCAATGCTACAAACAAGAACAACTCAATCAAACCTCATGTTCATCTTACAGGCAAGGTCCCTACTCGGCTGTGGCTTAGGTGATCGATTGATTGACCCTAATTTGAAGAACTACAACAAGGAAGAAATGGGGGCAATGATGATGGCGGCACGCCTCTGCCTGCTGTATTCTTCTTCGAGGAGGCCAACAATGAAAACTGTAAATCTTTTGATTAAGTCGACATTTCCATCAATGGGCGACTGTTCAGATTGATTAAACTAAACTTGTTTCTTGTTTGTTCAACAGATATTGAGGCTGTTTGAAGAACCAGGGTACCTGTTTAAGATGCAGAAGGTAAGAGATGAGGTCTTCAATGGCGGAAGTACAAAGGAAGAGGCCGATTTATGGAGATATGAAGAATCTGAGGACGAAGGAAGCATTATTATGGAAAATATTTGTTGATCTCTTGATTGAGTCTTGAGAGCTTACATAAATCTATGTATACATTTTGTTTTGGTGGATAACCCTTTGATTTTTCGTTTTTGAAAATTATAT

mRNA sequence

ATGTTGTCACTCATCAGGCCCAAGACTCAGAAGAAGAAAACAATTATCTTTGGTCTAAAATCTGACAATGGAAGCAGAGATATTCTTCTCTGGTTGCTTACGACAATCGTCAATCCAGGAGATTACGTTCTTGCTGTTCATGTTCAAGAGCCAAATGATAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAGCAGGTTGACTTACAGGTTAAGGTATGCAAAGCTAGTTCTTATATTTCGAACTTAACTCATCAAGTACGAGAGAGCTATGCGACTATTTTGGTACTTGGATGTAGCCTTTCAAGACCTAATTACTCAGTTTTCAATACCTGCTTGAAAGGCTTGCCTCCCACTTGCACTCTCATGATGATGGATAATCTAGGAAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAGCGGTTTTACAACCTTCTTGTTCATCAATTTCCAAGTATGCTATAGGATATCAACAAACCAATTCCTGTCACTTACAGAAGTTTTTGACCCTACCCTCTTCTCTACCTAATGAAAAACAAGAAATCTCCCTTGCCAACAAGAAAGCGCGGCCATTTCCCAACTTTGCACCTCGTGAACTGTTTCCTAGATCAGCACTGCAAGATTCAAAAGAAATCAAAAGGCAGTTTACATCCGAACAGCTCAACGGTGCAACCAACAATTTTGATCCTGCAATGGTGATTGGTGAAGGTGGACATAGTAAGGTGTATAGAGCTAACCTTGAAGATGGGCAAGCAGCAGCAGTGAAAGTTCTAAGCTCAGATTCTTCATCCGAAGATCTTGTTCAGGAAGTCGAAATCTTGTCTAAGATAAAACATGAGAACATAGTTCATATTATTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTCAAAGGAAGCCTAAAGCAATTCCTGAGAAAACTGGAGTGGAGGGAGAGAGTGAGGATTGCAATTGGAATAGCAAAAGCACTGAACTATCTTCATCATTCTTGCAACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAATTAGCAGATTTTGGAGAAGCAACAGTGCTCAAACAGTCTCAAAATGCACAATCCAACACAAAGCCATACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATGATGTATGGAAAGATTGATGAGAAAATAGATGTCTACTCCTATGGAGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCAAACAGATCAGAACAATCGTGCAAGTTTGGTACTCTGGGCAAGGTCCCTACTCGGCTGTGGCTTAGGTGATCGATTGATTGACCCTAATTTGAAGAACTACAACAAGGAAGAAATGGGGGCAATGATGATGGCGGCACGCCTCTGCCTGCTGTATTCTTCTTCGAGGAGGCCAACAATGAAAACTATATTGAGGCTGTTTGAAGAACCAGGGTACCTGTTTAAGATGCAGAAGGTAAGAGATGAGGTCTTCAATGGCGGAAGTACAAAGGAAGAGGCCGATTTATGGAGATATGAAGAATCTGAGGACGAAGGAAGCATTATTATGGAAAATATTTGTTGA

Coding sequence (CDS)

ATGTTGTCACTCATCAGGCCCAAGACTCAGAAGAAGAAAACAATTATCTTTGGTCTAAAATCTGACAATGGAAGCAGAGATATTCTTCTCTGGTTGCTTACGACAATCGTCAATCCAGGAGATTACGTTCTTGCTGTTCATGTTCAAGAGCCAAATGATAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAGCAGGTTGACTTACAGGTTAAGGTATGCAAAGCTAGTTCTTATATTTCGAACTTAACTCATCAAGTACGAGAGAGCTATGCGACTATTTTGGTACTTGGATGTAGCCTTTCAAGACCTAATTACTCAGTTTTCAATACCTGCTTGAAAGGCTTGCCTCCCACTTGCACTCTCATGATGATGGATAATCTAGGAAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAGCGGTTTTACAACCTTCTTGTTCATCAATTTCCAAGTATGCTATAGGATATCAACAAACCAATTCCTGTCACTTACAGAAGTTTTTGACCCTACCCTCTTCTCTACCTAATGAAAAACAAGAAATCTCCCTTGCCAACAAGAAAGCGCGGCCATTTCCCAACTTTGCACCTCGTGAACTGTTTCCTAGATCAGCACTGCAAGATTCAAAAGAAATCAAAAGGCAGTTTACATCCGAACAGCTCAACGGTGCAACCAACAATTTTGATCCTGCAATGGTGATTGGTGAAGGTGGACATAGTAAGGTGTATAGAGCTAACCTTGAAGATGGGCAAGCAGCAGCAGTGAAAGTTCTAAGCTCAGATTCTTCATCCGAAGATCTTGTTCAGGAAGTCGAAATCTTGTCTAAGATAAAACATGAGAACATAGTTCATATTATTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTCAAAGGAAGCCTAAAGCAATTCCTGAGAAAACTGGAGTGGAGGGAGAGAGTGAGGATTGCAATTGGAATAGCAAAAGCACTGAACTATCTTCATCATTCTTGCAACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAATTAGCAGATTTTGGAGAAGCAACAGTGCTCAAACAGTCTCAAAATGCACAATCCAACACAAAGCCATACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATGATGTATGGAAAGATTGATGAGAAAATAGATGTCTACTCCTATGGAGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCAAACAGATCAGAACAATCGTGCAAGTTTGGTACTCTGGGCAAGGTCCCTACTCGGCTGTGGCTTAGGTGATCGATTGATTGACCCTAATTTGAAGAACTACAACAAGGAAGAAATGGGGGCAATGATGATGGCGGCACGCCTCTGCCTGCTGTATTCTTCTTCGAGGAGGCCAACAATGAAAACTATATTGAGGCTGTTTGAAGAACCAGGGTACCTGTTTAAGATGCAGAAGGTAAGAGATGAGGTCTTCAATGGCGGAAGTACAAAGGAAGAGGCCGATTTATGGAGATATGAAGAATCTGAGGACGAAGGAAGCATTATTATGGAAAATATTTGTTGA

Protein sequence

MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTFHIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKGLPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLTLPSSLPNEKQEISLANKKARPFPNFAPRELFPRSALQDSKEIKRQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVRDEVFNGGSTKEEADLWRYEESEDEGSIIMENIC
Homology
BLAST of Spg001440 vs. NCBI nr
Match: XP_022140740.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charantia])

HSP 1 Score: 902.1 bits (2330), Expect = 2.3e-258
Identity = 458/551 (83.12%), Postives = 497/551 (90.20%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLS IR KT+KK+TIIFGLKSDNGSRDILLWLLTTI+NPGD VLAVHV+EPNDSFNPN+F
Sbjct: 1   MLSRIRSKTRKKRTIIFGLKSDNGSRDILLWLLTTIINPGDNVLAVHVREPNDSFNPNSF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV +A SYISNLT QVRE+YATILVLGCSLSRP+YSVF++CLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSEADSYISNLTRQVRENYATILVLGCSLSRPDYSVFSSCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLGRILLQKQGTSQQGSE+A+LQ S SS+SKYA GYQQ NSCHLQK LT
Sbjct: 121 LPPTCTLMVMDNLGRILLQKQGTSQQGSEKALLQSSQSSVSKYAAGYQQANSCHLQKSLT 180

Query: 181 LPSSLP------NEKQEISLANKKARPFPNFAPRELFPRSALQDSKEIKRQFTSEQLNGA 240
           LPSSLP      +EKQEISLANKKARPFP FA  E FPR ALQDS+   R+FTSEQLN A
Sbjct: 181 LPSSLPSSSRQASEKQEISLANKKARPFPGFASSEPFPRLALQDSRGTSREFTSEQLNRA 240

Query: 241 TNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVH 300
           TNNF+P MVIGEGGHSKVYRANLEDGQAAAVKVLSS SSS DLVQEVEI S+IKHENIV 
Sbjct: 241 TNNFNPVMVIGEGGHSKVYRANLEDGQAAAVKVLSSHSSSHDLVQEVEIFSEIKHENIVQ 300

Query: 301 IIGFCNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRD 360
           IIGFCND+NM+AIVY+LLKGSLKQ+LRK +WRERV IAIG+AKAL YLHHSCNPPIIHRD
Sbjct: 301 IIGFCNDRNMKAIVYSLLKGSLKQYLRKRKWRERVGIAIGVAKALTYLHHSCNPPIIHRD 360

Query: 361 VKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEK 420
           VKSSNILLFDNFQPQLADFGEATVL+QSQNA+SN  PYN+VGTFGYLAPEY+MYGK+DEK
Sbjct: 361 VKSSNILLFDNFQPQLADFGEATVLRQSQNAESNINPYNVVGTFGYLAPEYIMYGKVDEK 420

Query: 421 IDVYSYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLK-NYNKEEM 480
           IDVYSYGVVLLELITGKEAIQTDQN+  SLVL ARSLLG GLGDRLIDPNLK NYNK EM
Sbjct: 421 IDVYSYGVVLLELITGKEAIQTDQNSHGSLVLRARSLLGFGLGDRLIDPNLKGNYNKVEM 480

Query: 481 GAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVRDEVFNGGSTKEEADLWRYE 540
            AMMMAARLCLLYSSSRRP M+TIL+L EEP YLFKMQ+ RDE+FNGGST+EE  LWRY+
Sbjct: 481 RAMMMAARLCLLYSSSRRPIMETILKLLEEPEYLFKMQEARDELFNGGSTEEETYLWRYD 540

Query: 541 ESEDEGSIIME 545
           E + E S + E
Sbjct: 541 EPDGEESTLTE 551

BLAST of Spg001440 vs. NCBI nr
Match: XP_022970989.1 (PTI1-like tyrosine-protein kinase 1 [Cucurbita maxima])

HSP 1 Score: 826.6 bits (2134), Expect = 1.2e-235
Identity = 428/516 (82.95%), Postives = 458/516 (88.76%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+KK+TII GL SDNGSRDILLWLLTT+VNPGDYVLA+HVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIIGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV K + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSKTAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLG+IL Q QGTSQQGSERAVL+ S S       GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRS-ALQDSKEIKRQFTSEQLNGATNNF 240
           L SSL  NEKQ+IS   KK +PF NFA  EL P S +LQ  K IKRQFTSEQLN ATNN 
Sbjct: 181 LRSSLQANEKQKIS---KKEKPFLNFALHELLPSSRSLQYPKRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDS S+D+VQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQAIVYNLLKGSL Q+LRKLEW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAIVYNLLKGSLNQYLRKLEWKERVAIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLFDNFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFDNFQPQLADFGEATELKQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
           AAR CLL SSSRRPTMKTILRLFEE GYLFKMQKV+
Sbjct: 481 AARFCLLDSSSRRPTMKTILRLFEEQGYLFKMQKVQ 506

BLAST of Spg001440 vs. NCBI nr
Match: XP_023532060.1 (PTI1-like tyrosine-protein kinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 3.6e-235
Identity = 426/516 (82.56%), Postives = 458/516 (88.76%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+K++TII GL SDNGSRDILLWLLTT+VNPGDYVLA+HVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKRRTIIVGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV + + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSETAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLG+IL Q QGTSQQGSERAVL+ S S       GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRSALQD-SKEIKRQFTSEQLNGATNNF 240
           LPSSL  NE+Q+IS   KKA+PF NFAP EL P S  Q  S  IKRQFTSEQLN ATNN 
Sbjct: 181 LPSSLQENERQKIS---KKAQPFLNFAPHELLPSSKSQQYSTRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDS S+D+VQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQAIVYNLLKGSL Q+LRKLEW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAIVYNLLKGSLNQYLRKLEWKERVGIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLF NFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFGNFQPQLADFGEATQLKQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
           AARLCLL SSSRRPTMK ILRLFEE GYLFKMQKV+
Sbjct: 481 AARLCLLDSSSRRPTMKKILRLFEEQGYLFKMQKVQ 506

BLAST of Spg001440 vs. NCBI nr
Match: KAG6604957.1 (Alanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 813.9 bits (2101), Expect = 8.3e-232
Identity = 423/514 (82.30%), Postives = 454/514 (88.33%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+KK+TII GL SDNGSRDILLWLLTT+VNPGDYVLA+HVQEPNDSF+PNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFSPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV + + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLGRIL Q QGTSQQGSER       SS S    GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER-------SSHS----GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRSALQD-SKEIKRQFTSEQLNGATNNF 240
           LPSSL  NEKQ+IS   KKA+ F NFAP EL P S  Q  S  IKRQFTSEQLN ATNN 
Sbjct: 181 LPSSLQANEKQKIS---KKAQAFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAM+IGEGGHSKVYRANLEDGQAAAVK+LSSDS S++LVQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQAIVYNLLKGSL Q+LRK+EW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAIVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLFDNFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFDNFQPQLADFGEATELKQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQK 513
           AARLCLL SSSRRPTMKTILRLFEE GYLFKMQK
Sbjct: 481 AARLCLLDSSSRRPTMKTILRLFEEQGYLFKMQK 500

BLAST of Spg001440 vs. NCBI nr
Match: XP_022947700.1 (PTI1-like tyrosine-protein kinase 1 [Cucurbita moschata])

HSP 1 Score: 811.2 bits (2094), Expect = 5.4e-231
Identity = 421/516 (81.59%), Postives = 453/516 (87.79%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+KK+TII GL SDNGSRDILLWLLT +VNPGDYVLA+HVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTALVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV + + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLGRIL Q QGTSQQGSER       SS S    GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER-------SSHS----GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRSALQD-SKEIKRQFTSEQLNGATNNF 240
           LPSSL  NEKQ+IS   KKA+PF NFAP EL P S  Q  S  IKRQFTSEQLN ATNN 
Sbjct: 181 LPSSLQANEKQKIS---KKAQPFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAM+IGEGGHSKVYRANLEDGQAAAVK+LSSDS S++LVQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQA+VYNLLKGSL Q+LRK+EW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAVVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLF NFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFGNFQPQLADFGEATELKQSQNGQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
           AARLCLL SSSRRPTMKTILRL EE GYLFKM KV+
Sbjct: 481 AARLCLLDSSSRRPTMKTILRLLEEQGYLFKMLKVQ 502

BLAST of Spg001440 vs. ExPASy Swiss-Prot
Match: Q9SGY7 (Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thaliana OX=3702 GN=PERK11 PE=2 SV=2)

HSP 1 Score: 212.6 bits (540), Expect = 1.1e-53
Identity = 137/349 (39.26%), Postives = 203/349 (58.17%), Query Frame = 0

Query: 212 PRSALQDSKEIKRQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSD 271
           P SA+  + +I   FT E+L+  T  F  + V+GEGG   VY+  L +G+  A+K L S 
Sbjct: 346 PDSAVIGTSKI--HFTYEELSQITEGFCKSFVVGEGGFGCVYKGILFEGKPVAIKQLKSV 405

Query: 272 SSS--EDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLLKGSLKQF------LRKL 331
           S+    +   EVEI+S++ H ++V ++G+C  +  + ++Y  +  +   +      L  L
Sbjct: 406 SAEGYREFKAEVEIISRVHHRHLVSLVGYCISEQHRFLIYEFVPNNTLDYHLHGKNLPVL 465

Query: 332 EWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQ 391
           EW  RVRIAIG AK L YLH  C+P IIHRD+KSSNILL D F+ Q+ADFG A   + + 
Sbjct: 466 EWSRRVRIAIGAAKGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFGLA---RLND 525

Query: 392 NAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQN-NRA 451
            AQS+     ++GTFGYLAPEY   GK+ ++ DV+S+GVVLLELITG++ + T Q     
Sbjct: 526 TAQSHIST-RVMGTFGYLAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEE 585

Query: 452 SLVLWARSLL--GCGLGD--RLIDPNLKN-YNKEEMGAMMMAARLCLLYSSSRRPTMKTI 511
           SLV WAR  L      GD   ++DP L+N Y + E+  M+  A  C+ +S+ +RP M  +
Sbjct: 586 SLVEWARPRLIEAIEKGDISEVVDPRLENDYVESEVYKMIETAASCVRHSALKRPRMVQV 645

Query: 512 LRLFEEPGYLFKMQ---KV-RDEVFNGGSTKEEADLWR--YEESEDEGS 541
           +R  +    L  +    KV +  V++ G    E  ++R   E+S D G+
Sbjct: 646 VRALDTRDDLSDLTNGVKVGQSRVYDSGQYSNEIRIFRRASEDSSDLGT 688

BLAST of Spg001440 vs. ExPASy Swiss-Prot
Match: Q5XF57 (Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis thaliana OX=3702 GN=At5g57670 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.4e-53
Identity = 157/523 (30.02%), Postives = 265/523 (50.67%), Query Frame = 0

Query: 25  SRDILLWLLTTIVNPGDYVLAVHV---QEP---------NDSFNPNTFHIHEDI------ 84
           S+++L W +  +  P D V+A+H+   +EP                  H+   +      
Sbjct: 23  SQNVLSWAINVLAKPSDTVVALHLLVGEEPRKLPMKKKKRTQIRHAKAHVISMLGEFAYT 82

Query: 85  CKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRP---NYSVFNTCLKGL-- 144
           C   QV+L+ KV  +S+    L  +V+   A  LV    LSRP    + ++N   + +  
Sbjct: 83  CCHNQVNLEAKVGFSSNIGRGLIDEVKSISAHYLV----LSRPTTHEFRIWNDIKRYVSD 142

Query: 145 --PPTCTLMMMDNLGR--------ILLQKQGTSQQGSERAVLQP-SCSSISKYAIGYQQT 204
             P +C+++++ N  +          +     S++ S R+VL   S  S+S         
Sbjct: 143 FAPSSCSVVLVGNQRKPHKDCYSDSAISLDINSEKYSPRSVLNTLSRDSLSSSGDDASSF 202

Query: 205 NSCHLQKFLTLPSSLPNEKQEISLANKKARPFPNFAPRELFPRSALQDS---KEIKRQFT 264
           N   +      PS  P  K  IS     +    N +P   +  S  ++    + + + FT
Sbjct: 203 NGSMVSSSFASPSDKPKHK-SISPYKFISSLIMN-SPLRKWRGSETKNKPKPQPLIQCFT 262

Query: 265 SEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDS----SSEDLVQEVEI 324
             +++ ATN+F    ++G GG+S+VYR +L DG+  AVK L+ +S      ++ + E+ I
Sbjct: 263 YNEISKATNDFHQGNIVGIGGYSEVYRGDLWDGRRIAVKRLAKESGDMNKEKEFLTELGI 322

Query: 325 LSKIKHENIVHIIGFCNDKNMQAIVYNLLKGSLKQFLRK-----LEWRERVRIAIGIAKA 384
           +S + H N   ++G C +K +  +      G+L   L +     L+W  R +IA+G+A+ 
Sbjct: 323 ISHVSHPNTALLLGCCVEKGLYLVFRFSENGTLYSALHENENGSLDWPVRYKIAVGVARG 382

Query: 385 LNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTF 444
           L+YLH  CN  IIHRD+KSSN+LL  +++PQ+ DFG A   K   N  ++     + GTF
Sbjct: 383 LHYLHKRCNHRIIHRDIKSSNVLLGPDYEPQITDFGLA---KWLPNKWTHHAVIPVEGTF 442

Query: 445 GYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGD 501
           GYLAPE +M G IDEK D+Y++G++LLE+ITG+  +   Q +   ++LWA+  +  G   
Sbjct: 443 GYLAPESLMQGTIDEKTDIYAFGILLLEIITGRRPVNPTQKH---ILLWAKPAMETGNTS 502

BLAST of Spg001440 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.5e-53
Identity = 116/284 (40.85%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 224 RQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSED--LVQEV 283
           + FT  +L  AT+ F    V+GEGG  +VY+ ++EDG   AVK+L+ D+ + D   + EV
Sbjct: 335 KTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIAEV 394

Query: 284 EILSKIKHENIVHIIGFCNDKNMQAIVYNLL-KGSLKQFLRK--LEWRERVRIAIGIAKA 343
           E+LS++ H N+V +IG C +   + ++Y L+  GS++  L +  L+W  R++IA+G A+ 
Sbjct: 395 EMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLHEGTLDWDARLKIALGAARG 454

Query: 344 LNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTF 403
           L YLH   NP +IHRD K+SN+LL D+F P+++DFG A    +     S      ++GTF
Sbjct: 455 LAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREATEGSQHIST----RVMGTF 514

Query: 404 GYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQ-NNRASLVLWARSLLGCGLG 463
           GY+APEY M G +  K DVYSYGVVLLEL+TG+  +   Q +   +LV WAR LL    G
Sbjct: 515 GYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLANREG 574

Query: 464 -DRLIDPNLK-NYNKEEMGAMMMAARLCLLYSSSRRPTMKTILR 500
            ++L+DP L   YN ++M  +   A +C+    S RP M  +++
Sbjct: 575 LEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQ 614

BLAST of Spg001440 vs. ExPASy Swiss-Prot
Match: Q9SX31 (Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 GN=PERK9 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.2e-53
Identity = 133/325 (40.92%), Postives = 181/325 (55.69%), Query Frame = 0

Query: 223 KRQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVL--SSDSSSEDLVQE 282
           K  F+ E+L  ATN F    ++GEGG   VY+  L DG+  AVK L         +   E
Sbjct: 362 KALFSYEELVKATNGFSQENLLGEGGFGCVYKGILPDGRVVAVKQLKIGGGQGDREFKAE 421

Query: 283 VEILSKIKHENIVHIIGFCNDKNMQAIVYNLLKGSLKQF-----LRKLEWRERVRIAIGI 342
           VE LS+I H ++V I+G C   + + ++Y+ +  +   F        L+W  RV+IA G 
Sbjct: 422 VETLSRIHHRHLVSIVGHCISGDRRLLIYDYVSNNDLYFHLHGEKSVLDWATRVKIAAGA 481

Query: 343 AKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIV 402
           A+ L YLH  C+P IIHRD+KSSNILL DNF  +++DFG A  L    N    T+   ++
Sbjct: 482 ARGLAYLHEDCHPRIIHRDIKSSNILLEDNFDARVSDFGLAR-LALDCNTHITTR---VI 541

Query: 403 GTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQN-NRASLVLWARSLLGC 462
           GTFGY+APEY   GK+ EK DV+S+GVVLLELITG++ + T Q     SLV WAR L+  
Sbjct: 542 GTFGYMAPEYASSGKLTEKSDVFSFGVVLLELITGRKPVDTSQPLGDESLVEWARPLISH 601

Query: 463 GLG----DRLIDPNL-KNYNKEEMGAMMMAARLCLLYSSSRRPTMKTILRLFEE---PGY 522
            +     D L DP L  NY + EM  M+ AA  C+ + +++RP M  I+R FE       
Sbjct: 602 AIETEEFDSLADPKLGGNYVESEMFRMIEAAGACVRHLATKRPRMGQIVRAFESLAAEDL 661

Query: 523 LFKMQKVRDEVFNGGSTKEEADLWR 532
              M+    EVFN      E  L+R
Sbjct: 662 TNGMRLGESEVFNSAQQSAEIRLFR 682

BLAST of Spg001440 vs. ExPASy Swiss-Prot
Match: Q9FFW5 (Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 GN=PERK8 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 4.2e-53
Identity = 121/306 (39.54%), Postives = 182/306 (59.48%), Query Frame = 0

Query: 215 ALQDSKEIKRQ---FTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSD 274
           A  DS  +  Q   F+ ++L+  T+ F    ++GEGG   VY+  L DG+  AVK L   
Sbjct: 313 ASSDSGMVSNQRSWFSYDELSQVTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIG 372

Query: 275 SS--SEDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLLKGSLKQF------LRKL 334
            S    +   EVEI+S++ H ++V ++G+C  +  + +VY+ +  +   +         +
Sbjct: 373 GSQGEREFKAEVEIISRVHHRHLVTLVGYCISEQHRLLVYDYVPNNTLHYHLHAPGRPVM 432

Query: 335 EWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQ-S 394
            W  RVR+A G A+ + YLH  C+P IIHRD+KSSNILL ++F+  +ADFG A + ++  
Sbjct: 433 TWETRVRVAAGAARGIAYLHEDCHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQELD 492

Query: 395 QNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQN-NR 454
            N   +T+   ++GTFGY+APEY   GK+ EK DVYSYGV+LLELITG++ + T Q    
Sbjct: 493 LNTHVSTR---VMGTFGYMAPEYATSGKLSEKADVYSYGVILLELITGRKPVDTSQPLGD 552

Query: 455 ASLVLWARSLLGCGLG----DRLIDPNL-KNYNKEEMGAMMMAARLCLLYSSSRRPTMKT 503
            SLV WAR LLG  +     D L+DP L KN+   EM  M+ AA  C+ +S+++RP M  
Sbjct: 553 ESLVEWARPLLGQAIENEEFDELVDPRLGKNFIPGEMFRMVEAAAACVRHSAAKRPKMSQ 612

BLAST of Spg001440 vs. ExPASy TrEMBL
Match: A0A6J1CFZ7 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charantia OX=3673 GN=LOC111011329 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 458/551 (83.12%), Postives = 497/551 (90.20%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLS IR KT+KK+TIIFGLKSDNGSRDILLWLLTTI+NPGD VLAVHV+EPNDSFNPN+F
Sbjct: 1   MLSRIRSKTRKKRTIIFGLKSDNGSRDILLWLLTTIINPGDNVLAVHVREPNDSFNPNSF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV +A SYISNLT QVRE+YATILVLGCSLSRP+YSVF++CLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSEADSYISNLTRQVRENYATILVLGCSLSRPDYSVFSSCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLGRILLQKQGTSQQGSE+A+LQ S SS+SKYA GYQQ NSCHLQK LT
Sbjct: 121 LPPTCTLMVMDNLGRILLQKQGTSQQGSEKALLQSSQSSVSKYAAGYQQANSCHLQKSLT 180

Query: 181 LPSSLP------NEKQEISLANKKARPFPNFAPRELFPRSALQDSKEIKRQFTSEQLNGA 240
           LPSSLP      +EKQEISLANKKARPFP FA  E FPR ALQDS+   R+FTSEQLN A
Sbjct: 181 LPSSLPSSSRQASEKQEISLANKKARPFPGFASSEPFPRLALQDSRGTSREFTSEQLNRA 240

Query: 241 TNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVH 300
           TNNF+P MVIGEGGHSKVYRANLEDGQAAAVKVLSS SSS DLVQEVEI S+IKHENIV 
Sbjct: 241 TNNFNPVMVIGEGGHSKVYRANLEDGQAAAVKVLSSHSSSHDLVQEVEIFSEIKHENIVQ 300

Query: 301 IIGFCNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRD 360
           IIGFCND+NM+AIVY+LLKGSLKQ+LRK +WRERV IAIG+AKAL YLHHSCNPPIIHRD
Sbjct: 301 IIGFCNDRNMKAIVYSLLKGSLKQYLRKRKWRERVGIAIGVAKALTYLHHSCNPPIIHRD 360

Query: 361 VKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEK 420
           VKSSNILLFDNFQPQLADFGEATVL+QSQNA+SN  PYN+VGTFGYLAPEY+MYGK+DEK
Sbjct: 361 VKSSNILLFDNFQPQLADFGEATVLRQSQNAESNINPYNVVGTFGYLAPEYIMYGKVDEK 420

Query: 421 IDVYSYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLK-NYNKEEM 480
           IDVYSYGVVLLELITGKEAIQTDQN+  SLVL ARSLLG GLGDRLIDPNLK NYNK EM
Sbjct: 421 IDVYSYGVVLLELITGKEAIQTDQNSHGSLVLRARSLLGFGLGDRLIDPNLKGNYNKVEM 480

Query: 481 GAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVRDEVFNGGSTKEEADLWRYE 540
            AMMMAARLCLLYSSSRRP M+TIL+L EEP YLFKMQ+ RDE+FNGGST+EE  LWRY+
Sbjct: 481 RAMMMAARLCLLYSSSRRPIMETILKLLEEPEYLFKMQEARDELFNGGSTEEETYLWRYD 540

Query: 541 ESEDEGSIIME 545
           E + E S + E
Sbjct: 541 EPDGEESTLTE 551

BLAST of Spg001440 vs. ExPASy TrEMBL
Match: A0A6J1I7A3 (PTI1-like tyrosine-protein kinase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469794 PE=4 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 6.0e-236
Identity = 428/516 (82.95%), Postives = 458/516 (88.76%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+KK+TII GL SDNGSRDILLWLLTT+VNPGDYVLA+HVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIIGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV K + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSKTAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLG+IL Q QGTSQQGSERAVL+ S S       GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRS-ALQDSKEIKRQFTSEQLNGATNNF 240
           L SSL  NEKQ+IS   KK +PF NFA  EL P S +LQ  K IKRQFTSEQLN ATNN 
Sbjct: 181 LRSSLQANEKQKIS---KKEKPFLNFALHELLPSSRSLQYPKRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDS S+D+VQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQAIVYNLLKGSL Q+LRKLEW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAIVYNLLKGSLNQYLRKLEWKERVAIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLFDNFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFDNFQPQLADFGEATELKQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
           AAR CLL SSSRRPTMKTILRLFEE GYLFKMQKV+
Sbjct: 481 AARFCLLDSSSRRPTMKTILRLFEEQGYLFKMQKVQ 506

BLAST of Spg001440 vs. ExPASy TrEMBL
Match: A0A6J1G7M9 (PTI1-like tyrosine-protein kinase 1 OS=Cucurbita moschata OX=3662 GN=LOC111451486 PE=4 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.6e-231
Identity = 421/516 (81.59%), Postives = 453/516 (87.79%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLSLI+PKT+KK+TII GL SDNGSRDILLWLLT +VNPGDYVLA+HVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTALVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHEDICKSKQVDLQVKV + + YISNLT QVRESYA+ILVLGCSLSRP+YSVF+TCLKG
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTCTLM+MDNLGRIL Q QGTSQQGSER       SS S    GYQQTN   LQK  T
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER-------SSHS----GYQQTNIRDLQKSST 180

Query: 181 LPSSL-PNEKQEISLANKKARPFPNFAPRELFPRSALQD-SKEIKRQFTSEQLNGATNNF 240
           LPSSL  NEKQ+IS   KKA+PF NFAP EL P S  Q  S  IKRQFTSEQLN ATNN 
Sbjct: 181 LPSSLQANEKQKIS---KKAQPFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNYATNNL 240

Query: 241 DPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSSSEDLVQEVEILSKIKHENIVHIIGF 300
           DPAM+IGEGGHSKVYRANLEDGQAAAVK+LSSDS S++LVQEVEILSKIKHENIV  IGF
Sbjct: 241 DPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIVQFIGF 300

Query: 301 CNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSS 360
           C+DKNMQA+VYNLLKGSL Q+LRK+EW+ERV IAIG+AKALN+LHHSCNPPIIHRDVKSS
Sbjct: 301 CSDKNMQAVVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHRDVKSS 360

Query: 361 NILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVY 420
           NILLF NFQPQLADFGEAT LKQSQN QSNTKP  IVGTFGYLAPEY+MYGKIDEKID+Y
Sbjct: 361 NILLFGNFQPQLADFGEATELKQSQNGQSNTKPCKIVGTFGYLAPEYIMYGKIDEKIDIY 420

Query: 421 SYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLKNYNKEEMGAMMM 480
           SYGVVLLELITGKEAIQT+QN+  SLVLWAR LL CG G++LIDPNLKNYNKEEM AM+M
Sbjct: 421 SYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEEMEAMLM 480

Query: 481 AARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
           AARLCLL SSSRRPTMKTILRL EE GYLFKM KV+
Sbjct: 481 AARLCLLDSSSRRPTMKTILRLLEEQGYLFKMLKVQ 502

BLAST of Spg001440 vs. ExPASy TrEMBL
Match: A0A5J5BWU5 (Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_020959 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.4e-183
Identity = 337/552 (61.05%), Postives = 432/552 (78.26%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLS +R +T KKKT + GLKSDN SR++LL LL  +V  GDYVLA+HVQEPND F+PNTF
Sbjct: 12  MLSFLRTRTTKKKTFVVGLKSDNCSREMLLRLLNLVVMSGDYVLAIHVQEPNDIFDPNTF 71

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHED+CKSKQVD QVKVC  +SYI+ LTHQVR ++ATILVLGCS   P  SV   CLK 
Sbjct: 72  HIHEDLCKSKQVDFQVKVCIGNSYIAELTHQVRINFATILVLGCSTPWPKDSVVANCLKA 131

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQGSERAVLQPSCSSISKYAIGYQQTNSCHLQKFLT 180
           LPPTC+L++MDN GR+L+Q+ GTSQQGS   VLQ S S++S++    Q   S  ++K LT
Sbjct: 132 LPPTCSLLVMDNGGRVLVQRPGTSQQGSTNKVLQSSLSAVSRFPNCEQSGTSRQIKKSLT 191

Query: 181 LPSSLPNE--KQEISLANKKAR---PFPNFAPRELFPRSALQDSKEIKRQFTSEQLNGAT 240
           +PSS  +   +Q  ++  ++ R     P+F  +++  + A  ++K    +FTS +LN AT
Sbjct: 192 MPSSSTSSLLRQTENMRQQRVRKSLQLPDFMTQKMLQKLATLETKGSGSRFTSRELNCAT 251

Query: 241 NNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVL-SSDSSSEDLVQEVEILSKIKHENIVH 300
            NF   MVIGEGG+SKVYRAN+EDGQAAAVKVL ++ SS+EDL +EVE+LS +KHENI+ 
Sbjct: 252 YNFSTEMVIGEGGNSKVYRANIEDGQAAAVKVLKTTPSSAEDLFREVEMLSGLKHENIIQ 311

Query: 301 IIGFCNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRD 360
           +IG+C  K + A+VYNLLKGSL+Q L +L+W ER+ IAIG+AKAL+YL HSC+PPIIHRD
Sbjct: 312 LIGYCYSKELHAVVYNLLKGSLRQKLNQLKWSERMMIAIGVAKALDYL-HSCSPPIIHRD 371

Query: 361 VKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEK 420
           +KSSNILL DN QP L+DFG A V +Q+Q + +  KP+++VGTFGYLAPEYMMYGK+DEK
Sbjct: 372 IKSSNILLSDNCQPVLSDFGAAMVHQQTQQSSAYAKPFHVVGTFGYLAPEYMMYGKVDEK 431

Query: 421 IDVYSYGVVLLELITGKEAIQTDQ-NNRASLVLWARSLLGCGLGDRLIDPNLK-NYNKEE 480
           +DVYSYGVVLLELITGKEAIQT+Q +N  SLVLWARSLLGCGL +RLIDP LK +YNK+E
Sbjct: 432 VDVYSYGVVLLELITGKEAIQTNQTSNHESLVLWARSLLGCGLCERLIDPYLKEDYNKDE 491

Query: 481 MGAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVRDEVFNGGSTKEEADLWRY 540
           M  MM+AARLCLL+SSSRRP MKTILRLFEEP +  KMQ+ ++E+ N  S++ E+ LWR 
Sbjct: 492 MKTMMIAARLCLLHSSSRRPPMKTILRLFEEPEHWLKMQRKKEELLNRISSRGESGLWRN 551

Query: 541 EESEDEGSIIME 545
           ++S+  G+++++
Sbjct: 552 DDSDASGALLVD 562

BLAST of Spg001440 vs. ExPASy TrEMBL
Match: A0A6P4AVL8 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107427346 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.4e-181
Identity = 343/555 (61.80%), Postives = 420/555 (75.68%), Query Frame = 0

Query: 1   MLSLIRPKTQKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTF 60
           MLS  R K  K + IIFGLKSDN SR+ILL LL  +V P D VL +HVQ+ +D+F+PNTF
Sbjct: 12  MLS-FRSKKVKNRNIIFGLKSDNSSREILLKLLAVVVKPEDNVLVIHVQKEDDAFDPNTF 71

Query: 61  HIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLSRPNYSVFNTCLKG 120
           HIHED+CKSK+VD QVKVC   SYI+ LTHQVR SYA+IL LG SLS  NYS  N CLKG
Sbjct: 72  HIHEDLCKSKKVDFQVKVCNGESYITVLTHQVRLSYASILALGSSLSGLNYSAVNYCLKG 131

Query: 121 LPPTCTLMMMDNLGRILLQKQGTSQQ-GSERAVLQPSCSSISKYAIGYQQTNSCHLQKFL 180
           LPPTCTL++M+N GRI+++ QGTSQQ GS+RAVLQ S SS S Y    Q  ++  LQK L
Sbjct: 132 LPPTCTLLVMNNAGRIIIKSQGTSQQGGSKRAVLQSSFSS-SCYNRVPQSVHNSRLQKSL 191

Query: 181 TLP-----SSLPNEKQEISLANKKARPFPNFAPRE-LFPRSALQDSKEIKRQFTSEQLNG 240
           T+P     SS+    + +     K    PN   ++ L  R AL +S+   R FT E++  
Sbjct: 192 TVPYSSTSSSIQQTNKPVLFGASKTAQVPNLMMKQKLSKRLALLESEGSSRHFTMEEIRW 251

Query: 241 ATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVL-SSDSSSEDLVQEVEILSKIKHENI 300
           ATNNF P ++IGEGGHS VYRA  EDG+AAAVKVL +S  + EDL++EV++LS IKHENI
Sbjct: 252 ATNNFSPVVLIGEGGHSNVYRALFEDGKAAAVKVLKTSYHAVEDLLREVDLLSSIKHENI 311

Query: 301 VHIIGFCNDKNMQAIVYNLLKGSLKQFLRKLEWRERVRIAIGIAKALNYLHHSCNPPIIH 360
           V +IG+CN + MQAIVYNLL+GSL+Q LR L+WRER+R+AIG+AKAL+YLHHSCNPP++H
Sbjct: 312 VEVIGYCNSREMQAIVYNLLRGSLRQNLRHLKWRERMRVAIGVAKALDYLHHSCNPPVVH 371

Query: 361 RDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKID 420
           RDVKSSNILL D+FQPQL+DFG A +  QSQ A  N KP  +VGTFGYLAPEYMMYG++D
Sbjct: 372 RDVKSSNILLSDSFQPQLSDFGAAMLFHQSQQASENAKPCTVVGTFGYLAPEYMMYGEVD 431

Query: 421 EKIDVYSYGVVLLELITGKEAIQTDQNNRASLVLWARSLLGCGLGDRLIDPNLK-NYNKE 480
           EKIDVYSYGVVLLELITGKEA++TDQ    SLVLWARSLL  GL  RLIDP L  +YNKE
Sbjct: 432 EKIDVYSYGVVLLELITGKEAVKTDQAIHESLVLWARSLLSYGLFKRLIDPYLNGDYNKE 491

Query: 481 EMGAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVRDEVFNGGSTKEEADLWR 540
           EM  MM+AARLCL++SSS+RPT+KTILRLFEEP Y  KMQ+VRDE   G ++  E +  R
Sbjct: 492 EMEIMMIAARLCLVHSSSKRPTIKTILRLFEEPDYWIKMQRVRDEFLKGTTSIGETEPSR 551

Query: 541 YEESEDEGSIIMENI 547
            +ES +   ++++N+
Sbjct: 552 QDESVNSEDVLIDNL 564

BLAST of Spg001440 vs. TAIR 10
Match: AT2G16750.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 268.5 bits (685), Expect = 1.2e-71
Identity = 181/561 (32.26%), Postives = 301/561 (53.65%), Query Frame = 0

Query: 10  QKKKTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHV------QEPNDSFNPNTFHIH 69
           +++  I+ G+  D    ++L W L  +   GD V+ VHV         + S        +
Sbjct: 9   KQRNIILVGIPIDESGVEVLKWALEEVAKHGDCVVVVHVCFTYYRALKSKSSLDRYLKPY 68

Query: 70  EDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGC-SLSRPNYSVFNTCLKGLP 129
            + C +K+++L+ +V K +S +  L  + +   A  +V+G    S+ +  +   C K LP
Sbjct: 69  IEFCSTKKIELKGEVLKGNSVLGVLVKEAKRYNAMSVVVGVKQQSKLSLKIAKGCAKELP 128

Query: 130 PTCTLMMMDNLGRILLQKQGTSQ---------------------------QGSERAVLQP 189
            T  ++ + + G I+ ++    Q                            G E+  +  
Sbjct: 129 STTDILAI-HRGNIVFRRSNHYQLPLAQKISSRPSSELSEGFSDKDLAKTTGQEKRKISG 188

Query: 190 SCSSISKYAIGYQQTNSCHLQKFLTLPSSL-PNEKQEISLAN------KKARPFPNFAPR 249
              S+    +   QT    L +  TL + +  ++ ++IS+ N      ++    PN   +
Sbjct: 189 RSLSLPSVEV-VDQTPGWPLLRTSTLATPMVQHQTRKISVVNWVMSLPERFPHHPNQTCQ 248

Query: 250 ELFPRSALQD-SKEIKRQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKV 309
           + F    L+D  K+I R F+ + L  AT++F    +IG+GG ++VY+  LEDG+  AVK+
Sbjct: 249 QSFCDKQLKDILKDINRWFSYDVLKTATSDFSLENLIGKGGCNEVYKGFLEDGKGVAVKI 308

Query: 310 L--SSDSSSEDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNL-LKGSLKQFLR--- 369
           L  S   + ++ V EV I+S + H NI  +IG C   N    VYNL  KGSL++ L+   
Sbjct: 309 LKPSVKEAVKEFVHEVSIVSSLSHSNISPLIGVCVHYNDLISVYNLSSKGSLEETLQGKH 368

Query: 370 KLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQ 429
            L W ER++IAIG+ +AL+YLH+ C+ P+IHRDVKSSN+LL D F+PQL+DFG   +   
Sbjct: 369 VLRWEERLKIAIGLGEALDYLHNQCSNPVIHRDVKSSNVLLSDEFEPQLSDFG---LSMW 428

Query: 430 SQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQ-NN 489
              +   T   ++VGTFGYLAPEY MYGK+ +K+DVY++GVVLLELI+G+ +I +D    
Sbjct: 429 GSKSCRYTIQRDVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRTSISSDSPRG 488

Query: 490 RASLVLWARSLLGCGLGDRLIDPNLK-NYNKEEMGAMMMAARLCLLYSSSRRPTMKTILR 521
           + SLV+WA+ ++  G    L+DPN+   +++++   M++AA  CL  +++ RP +K IL+
Sbjct: 489 QESLVMWAKPMIEKGNAKELLDPNIAGTFDEDQFHKMVLAATHCLTRAATYRPNIKEILK 548

BLAST of Spg001440 vs. TAIR 10
Match: AT5G63940.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 250.0 bits (637), Expect = 4.4e-66
Identity = 190/633 (30.02%), Postives = 302/633 (47.71%), Query Frame = 0

Query: 15  IIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHVQEPNDSFNPNTFH----------IHE 74
           +I G+K D  SR++L W L  +  PGD ++A+HV + +   + +             ++E
Sbjct: 22  LIVGVKPDEWSREVLTWSLVNVARPGDRIVALHVLDYSLEGSTSLISLVRNFDTMLGVYE 81

Query: 75  DICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLS----RPNYSVFNTCLKG 134
             C  KQVDL++KV +  S    L  +V+   AT L++G S      R + S+   C + 
Sbjct: 82  SFCNLKQVDLKLKVFRGKSARKVLVQEVKSCGATSLIVGSSRKHHTIRSSASLAKYCARN 141

Query: 135 LPPTCTLMMMDNLGRILLQK--QGTSQQGSERAVLQPSCSS----ISKYAIG-------- 194
           L    ++  + + G+I+ ++        G +  +    C S    I    IG        
Sbjct: 142 LAKDVSVFAVKS-GKIMFRRVPNTNGADGPQMNIPSLVCGSPDIAIEAAKIGNSFSPART 201

Query: 195 ------------YQQTNSCHLQKFLTLPSSLPNEKQEISLAN------------------ 254
                        Q  +S  +   L L     NE    SL +                  
Sbjct: 202 SSRWTRTSRSSSLQSPDSLGVDNLLALVPVQTNETDSGSLESGPGWHFLRGLYGNNRKSW 261

Query: 255 -----KKA---------------------------------------------------- 314
                KKA                                                    
Sbjct: 262 TKVSAKKAVLQWVSRLRGRHSETVIYLDRKRSDSGCDEDCSSSIDGEDVSISRFGSELMQ 321

Query: 315 ---RPF--PNFAPRELFPRSALQDSKEIKRQFTSEQLNGATNNFDPAMVIGEGGHSKVYR 374
               PF   N  P EL      +      R FT E++   T+NF    ++GEGG+S VYR
Sbjct: 322 SPLSPFIGSNNIPEEL--EGLHEKYSSTCRLFTYEEVLSITSNFASENLVGEGGNSYVYR 381

Query: 375 ANLEDGQAAAVKVLSSD-SSSEDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLL- 434
            +L DG+  AVK+L       ++ + E+E+++ + H+NIV + GFC + N   +VY+ L 
Sbjct: 382 GDLPDGRELAVKILKPCLDVLKEFILEIEVITSVHHKNIVSLFGFCFENNNLMLVYDYLP 441

Query: 435 KGSLKQFL-------RKLEWRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDN 494
           +GSL++ L       +K  W ER ++A+G+A+AL+YLH++ +P +IHRDVKSSN+LL D+
Sbjct: 442 RGSLEENLHGNRKDAKKFGWMERYKVAVGVAEALDYLHNTHDPEVIHRDVKSSNVLLADD 501

Query: 495 FQPQLADFGEATVLKQSQNAQSNTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLL 515
           F+PQL+DFG A++   + +   +    +I GTFGYLAPEY M+GK+ +KIDVY++GVVLL
Sbjct: 502 FEPQLSDFGFASL---ASSTSQHVAGGDIAGTFGYLAPEYFMHGKVTDKIDVYAFGVVLL 561

BLAST of Spg001440 vs. TAIR 10
Match: AT1G77280.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 248.1 bits (632), Expect = 1.7e-65
Identity = 203/712 (28.51%), Postives = 327/712 (45.93%), Query Frame = 0

Query: 13  KTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAVHV-------QEPNDSF------NPNT 72
           +TI+ G+K D  SR++L W L  +  PGD V+A+H+       +  N S         + 
Sbjct: 48  RTILVGVKLDAPSRELLTWALVKVAEPGDTVIALHILGNEIVDRAGNSSLLSLVRTFDSV 107

Query: 73  FHIHEDICKSKQVDLQVKVCKASSYISNLTHQVRESYATILVLGCSLS----RPNYSVFN 132
             ++E  C  KQVDL++K+C+ SS    L  + +   AT +++G S S    R + SV  
Sbjct: 108 LDVYEGFCNLKQVDLKLKLCRGSSARKILVREAKSFSATKVLVGISKSHHAIRSSASVAK 167

Query: 133 TCLKGLPPTCTLMMMDNLGRILLQKQGTSQQ------------------GSERA------ 192
              K L   C ++ ++N G+IL QK+G+                     G E        
Sbjct: 168 YIAKKLSKDCWVIAVNN-GKILFQKEGSPSSTINQSQVVEETVIGFGYIGKEDVRRITLL 227

Query: 193 -VLQPS---------------------------CSSISKYAIGYQQTNSCHLQKF----- 252
            VLQ S                           C    +  +   +  +C +  F     
Sbjct: 228 NVLQRSVTLNKTTKVVSHSEEDSNSKEEEEDQACGQNLRQVLAAARLENCSVCGFDSLSP 287

Query: 253 --LTLPSSLP--------------NEKQEI--------------SLANKKARPFPNF--- 312
              T P  L               N+  EI               L  K   P P +   
Sbjct: 288 NDTTTPGKLSGASDFDRSEDDDECNKAMEIVPVNGSEDSGGSVTMLVRKLPEPRPGWPLL 347

Query: 313 -------------------------APREL------------------------------ 372
                                     PR+                               
Sbjct: 348 RRAVSTLGQSVTPHKIPVVQWALKLPPRDTKQLGYDSSEDNLSTLNALVPFGINSITNKS 407

Query: 373 ----FPRSALQDSKEIKRQFTS-------EQLNGATNNFDPAMVIGEGGHSKVYRANLED 432
                PR   ++ + +  +F+S       ++L   T+NF     IG+GG S+V+R  L +
Sbjct: 408 IPDNSPRKLPEELEGLYERFSSTCRFFKYKELVSVTSNFSADNFIGKGGSSRVFRGCLSN 467

Query: 433 GQAAAVKVL-SSDSSSEDLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLL-KGSLK 492
           G+  AVK+L  ++    D V E+EI++ + H+NI+ ++GFC + +   +VYN L +GSL+
Sbjct: 468 GRVVAVKILKQTEDVLNDFVAEIEIITTLHHKNIISLLGFCFEDHNLLLVYNYLSRGSLE 527

Query: 493 QFLRKLE-------WRERVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQL 537
           + L   +       W ER ++A+G+A+AL+YLH++ + P+IHRDVKSSNILL D+F+PQL
Sbjct: 528 ENLHGNKKDPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVKSSNILLSDDFEPQL 587

BLAST of Spg001440 vs. TAIR 10
Match: AT4G35030.3 (Protein kinase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 4.3e-61
Identity = 156/406 (38.42%), Postives = 231/406 (56.90%), Query Frame = 0

Query: 168 QQTNSCHLQK------FLTLPSSLPNEKQ----EISLANKKARPFPNFAPRELFPRSALQ 227
           QQ +  H +K       ++LP   PN +Q    E SL  K+              +  L+
Sbjct: 40  QQVHKWHTRKVSVVNWVMSLPERFPNHQQTLNYETSLIKKQI-------------KDILR 99

Query: 228 DSKEIKRQFTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDS--SSE 287
           D+    + F    L  AT++F    VIG+GG ++VYR  LEDG+  AVK+L S S  +  
Sbjct: 100 DN---NKWFNYNVLRKATSDFSQENVIGKGGCNEVYRGILEDGKGIAVKILKSSSKEAMT 159

Query: 288 DLVQEVEILSKIKHENIVHIIGFCNDKNMQAIVYNLLK-GSLKQFLR-------KLEWRE 347
           + V E+ I+S + H+NI  ++G C   N    VYNL   GSL++ L         L W E
Sbjct: 160 NFVHEINIISSLSHQNISPLLGVCVQDNELISVYNLSNTGSLEETLHGKQKGKYVLSWEE 219

Query: 348 RVRIAIGIAKALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQS 407
           R +IAIG+A+AL+YLH+ C+ P+IHRDVK+SN+LL    QPQL+DFG +     + +  S
Sbjct: 220 RFKIAIGLAEALDYLHNRCSKPVIHRDVKTSNVLLSLELQPQLSDFGLSMWGPTTSSRYS 279

Query: 408 NTKPYNIVGTFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQNNRA--SLV 467
                ++VGTFGYLAPEY MYGK+ +K+DVY++GVVLLELI+G+  I + QN R   SLV
Sbjct: 280 IQG--DVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRNPI-SPQNPRGQESLV 339

Query: 468 LWARSLLGCGLGDRLIDPNLKN-YNKEEMGAMMMAARLCLLYSSSRRPTMKTILRLFEEP 527
           +WA+ L+  G    L+DP++ + +++ +   M++AA  CL  S++ RP ++ ILRL  + 
Sbjct: 340 MWAKPLIDTGNLKVLLDPDVTDIFDESQFQRMVLAASHCLTRSATHRPNIRQILRLLRDE 399

Query: 528 GYLFK--MQK------VRDEVFNGGSTKEEADLWRYEESEDEGSII 543
               K  M++        DEV+   ST+   +L   E  +DE + I
Sbjct: 400 NEAGKWIMEEEGNEDCFDDEVYPNSSTELHLNLAMLEVEDDETASI 426

BLAST of Spg001440 vs. TAIR 10
Match: AT1G21590.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 230.7 bits (587), Expect = 2.8e-60
Identity = 124/300 (41.33%), Postives = 196/300 (65.33%), Query Frame = 0

Query: 226 FTSEQLNGATNNFDPAMVIGEGGHSKVYRANLEDGQAAAVKVLS-SDSSSEDLVQEVEIL 285
           FT ++L   T+NF     IG+GG S+V+R  L +G+  AVK+L  ++   +D V E++I+
Sbjct: 397 FTYKELVSVTSNFCADNFIGKGGSSRVFRGYLPNGREVAVKILKRTECVLKDFVAEIDII 456

Query: 286 SKIKHENIVHIIGFCNDKNMQAIVYNLL-KGSL-------KQFLRKLEWRERVRIAIGIA 345
           + + H+N++ ++G+C + N   +VYN L +GSL       K+ L    W ER ++A+GIA
Sbjct: 457 TTLHHKNVISLLGYCFENNNLLLVYNYLSRGSLEENLHGNKKDLVAFRWNERYKVAVGIA 516

Query: 346 KALNYLHHSCNPPIIHRDVKSSNILLFDNFQPQLADFGEATVLKQSQNAQSNTKPYNIVG 405
           +AL+YLH+    P+IHRDVKSSNILL D+F+PQL+DFG A   K +  + +     ++ G
Sbjct: 517 EALDYLHNDAPQPVIHRDVKSSNILLSDDFEPQLSDFGLA---KWASESTTQIICSDVAG 576

Query: 406 TFGYLAPEYMMYGKIDEKIDVYSYGVVLLELITGKEAIQTDQ-NNRASLVLWARSLLGCG 465
           TFGYLAPEY MYGK++ KIDVY+YGVVLLEL++G++ + ++    + SLV+WA+ +L   
Sbjct: 577 TFGYLAPEYFMYGKMNNKIDVYAYGVVLLELLSGRKPVNSESPKAQDSLVMWAKPILDDK 636

Query: 466 LGDRLIDPNLK-NYNKEEMGAMMMAARLCLLYSSSRRPTMKTILRLFEEPGYLFKMQKVR 515
              +L+D +L+ + N ++M  M +AA LC+ ++   RPTM  +L L +    + K  K++
Sbjct: 637 EYSQLLDSSLQDDNNSDQMEKMALAATLCIRHNPQTRPTMGMVLELLKGDVEMLKWAKLQ 693

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140740.12.3e-25883.12receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charanti... [more]
XP_022970989.11.2e-23582.95PTI1-like tyrosine-protein kinase 1 [Cucurbita maxima][more]
XP_023532060.13.6e-23582.56PTI1-like tyrosine-protein kinase 2 [Cucurbita pepo subsp. pepo][more]
KAG6604957.18.3e-23282.30Alanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosper... [more]
XP_022947700.15.4e-23181.59PTI1-like tyrosine-protein kinase 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SGY71.1e-5339.26Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thalian... [more]
Q5XF571.4e-5330.02Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis ... [more]
Q8RWW02.5e-5340.85Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=37... [more]
Q9SX313.2e-5340.92Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FFW54.2e-5339.54Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CFZ71.1e-25883.12receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charan... [more]
A0A6J1I7A36.0e-23682.95PTI1-like tyrosine-protein kinase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469794 ... [more]
A0A6J1G7M92.6e-23181.59PTI1-like tyrosine-protein kinase 1 OS=Cucurbita moschata OX=3662 GN=LOC11145148... [more]
A0A5J5BWU53.4e-18361.05Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_02... [more]
A0A6P4AVL81.4e-18161.80receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Zizip... [more]
Match NameE-valueIdentityDescription
AT2G16750.11.2e-7132.26Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT5G63940.14.4e-6630.02Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G77280.11.7e-6528.51Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G35030.34.3e-6138.42Protein kinase superfamily protein [more]
AT1G21590.12.8e-6041.33Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 238..501
e-value: 2.4E-30
score: 116.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 242..498
e-value: 4.1E-44
score: 150.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 238..506
score: 36.68269
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 213..311
e-value: 8.9E-26
score: 92.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 312..531
e-value: 3.2E-56
score: 191.9
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 239..431
e-value: 6.6E-25
score: 85.5
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 244..435
e-value: 4.9E-27
score: 93.0
NoneNo IPR availablePANTHERPTHR27001:SF155SERINE/THREONINE-PROTEIN KINASE CDL1-LIKE ISOFORM X1coord: 7..529
NoneNo IPR availablePANTHERPTHR27001OS01G0253100 PROTEINcoord: 7..529
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 350..362
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 244..266
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 222..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001440.1Spg001440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity