Spg001357 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001357 vs. NCBI nr
Match: XP_038902749.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida] >XP_038902750.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida] >XP_038902751.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida] >XP_038902753.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida] >XP_038902754.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida] >XP_038902755.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Benincasa hispida])

HSP 1 Score: 1342.4 bits (3473), Expect = 0.0e+00
Identity = 693/779 (88.96%), Postives = 726/779 (93.20%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           P+ESPLYTS  ATPEPLAFTEKS SQFSF SPSFSQ VDT GN+SPSPSP TSSRFQANH
Sbjct: 61  PIESPLYTSNIATPEPLAFTEKS-SQFSF-SPSFSQHVDTAGNLSPSPSPPTSSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRG+FA+KVEEKLPSPVIGT+TSSDIP SAT QTFERPQTLSFEGSSAPQEG WDYFF
Sbjct: 121 MQFRGTFAHKVEEKLPSPVIGTITSSDIPASATPQTFERPQTLSFEGSSAPQEGGWDYFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSFQDGNG+NNGGFEFENA G RYFKEEDGN E+GD+E KGSLHGGEESQNSED
Sbjct: 181 PSNNHEFSFQDGNGLNNGGFEFENAGGGRYFKEEDGNFEYGDKEGKGSLHGGEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVHDDGA N SPT+HT KS+ASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHDDGAANASPTLHTVKSIASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSVFGKA AKEDSIENTAV KD FSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSVVPFTSVFGKATAKEDSIENTAV-KDLFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE   LSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP
Sbjct: 361 HIRPIFPGKENPPLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VED SSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGGNSKEDVEDQSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PKID+TRAVIKDLHSRIRVG+HRIDSISKKIEELRDKELQPQLEELIE
Sbjct: 481 MLRHLESKEVGLPKIDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDKELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCH+ QLQIIKA S HG MKISMQSETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 GLSRMWEVMFDCHEHQLQIIKAASYHGTMKISMQSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
            AQK+YL SIDGWLLKCV+LPQK+SRGKRRAQAPSI+ Y PPPIYITCSVWLEKIN+LPT
Sbjct: 601 TAQKSYLHSIDGWLLKCVALPQKASRGKRRAQAPSIKTYGPPPIYITCSVWLEKINDLPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQDEASE 720
           KEVVDSIKDLA ETAR LPHQEKN GK K  KNLSILTSFKA ND ESMG NLLQ EASE
Sbjct: 661 KEVVDSIKDLAVETARLLPHQEKNPGKRKVGKNLSILTSFKAGNDSESMGNNLLQGEASE 720

Query: 721 SLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQSGI 780
           SL+SGFDHFRPSLVKFFE+LNNFA+ S++MYAEL  +IQD KS Y+Q KSQ+LEKQ+GI
Sbjct: 721 SLISGFDHFRPSLVKFFEKLNNFADSSVKMYAELGKTIQDFKSNYEQWKSQRLEKQTGI 776

BLAST of Spg001357 vs. NCBI nr
Match: XP_008449860.1 (PREDICTED: uncharacterized protein LOC103491612 [Cucumis melo] >XP_008449862.1 PREDICTED: uncharacterized protein LOC103491612 [Cucumis melo] >XP_008449863.1 PREDICTED: uncharacterized protein LOC103491612 [Cucumis melo] >KAA0040090.1 DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa] >TYK21716.1 DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 678/779 (87.03%), Postives = 718/779 (92.17%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SK+EEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKLEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY ST ATPEPLAFTEKS SQFSFSSPSFS  +DT GN SPSPSP TSSRFQANH
Sbjct: 61  PVESPLYASTFATPEPLAFTEKSASQFSFSSPSFSHHMDTAGNRSPSPSPPTSSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGSFA+KVEEKLPSPVIGTVTSSD PPSA  QT ERPQTLSFEGSSAPQEG WD+FF
Sbjct: 121 MQFRGSFAHKVEEKLPSPVIGTVTSSDTPPSAKPQTSERPQTLSFEGSSAPQEGTWDFFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSF DG+G+NNGG EFENA  +RYFKEEDGN E+GD+E K SLHG EESQNSED
Sbjct: 181 PSNNHEFSFHDGHGVNNGGVEFENAGDVRYFKEEDGNFEYGDKEGKSSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVHDDGA NTSPTMHT KSVASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHDDGASNTSPTMHTVKSVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSS+VP TSVFGKA AK++SIEN AVPKD FSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSLVPLTSVFGKATAKDESIENPAVPKDLFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE  SLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP
Sbjct: 361 HIRPIFPGKENPSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VED+SSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGVNSKEDVEDYSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PKID+TRAVIKDLHSRIRVG+HRIDSISKKIEELRD+ELQPQLEELIE
Sbjct: 481 MLRHLESKEVGLPKIDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDRELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCHK QLQIIKA S HGNMKISM SETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 GLSRMWEVMFDCHKNQLQIIKAASYHGNMKISMHSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
            AQK+YL SIDGWLLKCV+LP   SRGKRRAQ PSI+ + PPPIYITCSVWLEKIN LPT
Sbjct: 601 TAQKSYLHSIDGWLLKCVTLP--PSRGKRRAQTPSIKTFGPPPIYITCSVWLEKINELPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQDEASE 720
           KEVVDSIKDLAAETARFLPHQEKN GKGK AKNLS+LTSFK +ND ES+G NLLQ EASE
Sbjct: 661 KEVVDSIKDLAAETARFLPHQEKNPGKGKVAKNLSVLTSFKGNNDSESIGNNLLQGEASE 720

Query: 721 SLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQSGI 780
           SL+SGFDH RPSLVKFFE+LNNFA+ S++MYAEL  +IQ+ KS Y+Q K+Q++E Q+GI
Sbjct: 721 SLISGFDHLRPSLVKFFEKLNNFADSSVKMYAELGKTIQEFKSHYEQWKAQRMETQAGI 777

BLAST of Spg001357 vs. NCBI nr
Match: KAG7010215.1 (hypothetical protein SDJN02_27007 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1318.9 bits (3412), Expect = 0.0e+00
Identity = 675/779 (86.65%), Postives = 717/779 (92.04%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SK+EEDKALRLCRERKK VKQALHGRC+LATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 31  MGASSSKIEEDKALRLCRERKKLVKQALHGRCTLATAHAEYIQSLRCTGTALKIFVQPEG 90

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY STN TPEPLAFTEKSVSQ SFSSPSFSQRVDTV N+SPSPSP TSS+FQANH
Sbjct: 91  PVESPLYHSTNGTPEPLAFTEKSVSQLSFSSPSFSQRVDTVSNLSPSPSPPTSSQFQANH 150

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGS ++KVEEK PSPVIGT+TSSDIPPSAT QTFERPQ LSFEGSSAPQEGAWDYFF
Sbjct: 151 MQFRGSISHKVEEKFPSPVIGTITSSDIPPSATRQTFERPQALSFEGSSAPQEGAWDYFF 210

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
            S+NHEFSFQDG+G+NNG FEFENA GL+YFKE DGN E+GD EEKGSLHG EESQNSED
Sbjct: 211 SSDNHEFSFQDGSGVNNGEFEFENAGGLKYFKEADGNFENGDGEEKGSLHGEEESQNSED 270

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVH+DGA NTSPTMHT K+VASEPELVNQGKNHSPGLSPLRT
Sbjct: 271 EFDEPASETLVRSFENFNRVHNDGAANTSPTMHTVKTVASEPELVNQGKNHSPGLSPLRT 330

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSVFGKA AKEDSIENTAVPKDFFSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 331 TSSVVPFTSVFGKATAKEDSIENTAVPKDFFSSMKEIEILFIKASESGKEVPRMLEANKL 390

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           H+RPIFPGKE QSLSSTLLKSCFSCGDDPSV+REEPVQTATKYLTWHRT SS SSSSRNP
Sbjct: 391 HMRPIFPGKENQSLSSTLLKSCFSCGDDPSVLREEPVQTATKYLTWHRTASSHSSSSRNP 450

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE +EDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 451 LGGNSKEDIEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 510

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PK+D+TRAVIKDLHSRIRVG+HRIDSISKKIEELRDKELQPQLEELIE
Sbjct: 511 VLRHLESKEVGLPKVDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDKELQPQLEELIE 570

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
            LSRMWEVMFDCHK QLQ+IKA S HGNM ISMQSETRRHNTIYLETELASLSSSF KWI
Sbjct: 571 ALSRMWEVMFDCHKRQLQVIKAASYHGNMGISMQSETRRHNTIYLETELASLSSSFMKWI 630

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
           AAQK YLQ IDGWL  CVSLPQK S+GKRR QAPSIR Y PPPIY+TCSVWLEKI++LP 
Sbjct: 631 AAQKLYLQLIDGWLRTCVSLPQK-SKGKRRTQAPSIRTYGPPPIYVTCSVWLEKIDDLPM 690

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKG----AKNLSILTSFKADNDIESMGKNLLQD 720
           KEV+DSIKDLAAET RFLP QEKN GKGKG    AKNLSILTSFK DND ES+G NLLQD
Sbjct: 691 KEVIDSIKDLAAETTRFLPRQEKNLGKGKGKGKVAKNLSILTSFKGDNDSESLGNNLLQD 750

Query: 721 EASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEK 776
           +ASE L+SGFDHFR  LVKFFE LNNFAE S++MY+EL  +IQD KS Y+Q KSQ++EK
Sbjct: 751 KASERLISGFDHFRSRLVKFFENLNNFAEASVKMYSELGKTIQDFKSSYEQWKSQRVEK 808

BLAST of Spg001357 vs. NCBI nr
Match: XP_022986433.1 (nitrate regulatory gene2 protein [Cucurbita maxima])

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 676/779 (86.78%), Postives = 716/779 (91.91%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SKVEEDKALRLCRERKK VKQALHGRC+LATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKVEEDKALRLCRERKKLVKQALHGRCTLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY STNATPEPLAFTEKSVSQFSFSSPSFSQRVD V N+SPSPSP TSS+FQANH
Sbjct: 61  PVESPLYHSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDAVSNLSPSPSPPTSSQFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGS ++KVE KLPSPVIGT+TSSDIPPS T QTFERP+ LSFEGSSAPQEGAWDYFF
Sbjct: 121 MQFRGSISHKVEAKLPSPVIGTITSSDIPPSTTRQTFERPRALSFEGSSAPQEGAWDYFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
            S+NHEFSFQDG+G+NNG FEFENA GL+YFKEEDGN E+GD EEKGSLHG EESQNSED
Sbjct: 181 SSDNHEFSFQDGSGVNNGEFEFENAGGLKYFKEEDGNFENGDGEEKGSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEP+SETLVRSFENFNRV++DGA NTSPTMHT K+VASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPSSETLVRSFENFNRVYNDGAANTSPTMHTVKTVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSVFGKA AKEDSIENTAVP DFFSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSVVPFTSVFGKATAKEDSIENTAVPMDFFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE QSLSSTLLKSCFSCGDDPSV+REEPVQTATKYLTWHRT SS SS+SRNP
Sbjct: 361 HIRPIFPGKENQSLSSTLLKSCFSCGDDPSVLREEPVQTATKYLTWHRTASSHSSASRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGGNSKEDVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PK+D+TRAVIKDLHSRIRVG+HRIDSISKKIEELRDKELQPQLEELIE
Sbjct: 481 VLRHLESKEVGLPKVDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDKELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
            LSRMWEVMFDCHK QLQ+I+A S HGNM ISMQSETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 ALSRMWEVMFDCHKRQLQVIEAASYHGNMGISMQSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
           AAQK YLQ IDGWL  CVSLPQK S+GKRR QAPSIR Y PPPIYITCSVWLEKI++LP 
Sbjct: 601 AAQKLYLQLIDGWLRTCVSLPQK-SKGKRRTQAPSIRTYGPPPIYITCSVWLEKIDDLPM 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKG----AKNLSILTSFKADNDIESMGKNLLQD 720
           KEV+DSIKDLA ET RFLP QEKN GKGKG    AKNLSILTSFK DND ES+G NLLQD
Sbjct: 661 KEVIDSIKDLATETTRFLPRQEKNLGKGKGKGKVAKNLSILTSFKGDNDSESLGNNLLQD 720

Query: 721 EASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEK 776
           EASE L+SGFDHFR  LVKFFE LNNFAE S++MYAEL  +IQD KS Y+Q KSQ+LEK
Sbjct: 721 EASERLISGFDHFRSRLVKFFENLNNFAEASVKMYAELGKTIQDFKSSYEQWKSQRLEK 778

BLAST of Spg001357 vs. NCBI nr
Match: XP_023512458.1 (nitrate regulatory gene2 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 673/779 (86.39%), Postives = 714/779 (91.66%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SKVEEDKALRLCRERKK VKQALHGRC+LATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKVEEDKALRLCRERKKLVKQALHGRCTLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY STNATPEPLAFTEKSVSQ SFSSPSFSQRV+TV N+SPSPSP TSS+FQ NH
Sbjct: 61  PVESPLYHSTNATPEPLAFTEKSVSQLSFSSPSFSQRVNTVSNLSPSPSPPTSSQFQENH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGS ++KVEEKLPSPVIGT+TSSDIPPSAT QTFERPQ LSFEGSSAPQEGAWDYFF
Sbjct: 121 MQFRGSISHKVEEKLPSPVIGTITSSDIPPSATRQTFERPQALSFEGSSAPQEGAWDYFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
            S+NHEFSFQDG+G+NNG FEFENA GL+YFKE DGN E+GD EEKGSLHG EESQNSED
Sbjct: 181 SSDNHEFSFQDGSGVNNGEFEFENAGGLKYFKEADGNFENGDGEEKGSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVH+DGA NTSPTMHT K+VASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHNDGAANTSPTMHTVKTVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSV GKA  KEDSIENTAVPKDFFSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSVVPFTSVVGKATEKEDSIENTAVPKDFFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE QSLSSTLLKSCFSCGDDPSV+REEPVQTATKYLTWHRT SS SSSSRNP
Sbjct: 361 HIRPIFPGKENQSLSSTLLKSCFSCGDDPSVLREEPVQTATKYLTWHRTASSHSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGGNSKEDVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PK+D+TRAVIKDLHSRI VG+HRIDSISKKIEELRDKELQPQL+ELIE
Sbjct: 481 VLRHLESKEVGLPKVDKTRAVIKDLHSRIGVGVHRIDSISKKIEELRDKELQPQLDELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
            LSRMWEVMFDCHK QLQ+I A S+HGNM IS+QSETRRHNTIYLETELASLS SF KWI
Sbjct: 541 ALSRMWEVMFDCHKRQLQVIIAASSHGNMGISIQSETRRHNTIYLETELASLSLSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
           AAQK YLQ IDGWL  CVSLPQK S+GKRR QAPSIR Y PPPIY+TCSVWLEKI++LP 
Sbjct: 601 AAQKLYLQLIDGWLRTCVSLPQK-SKGKRRTQAPSIRTYGPPPIYVTCSVWLEKIDDLPM 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKN----QGKGKGAKNLSILTSFKADNDIESMGKNLLQD 720
           KEV+DSIKDLAAET RFLP QEKN    +GKGK AKNLSILTSFK DND ES+G NLLQD
Sbjct: 661 KEVIDSIKDLAAETTRFLPRQEKNLGKEKGKGKVAKNLSILTSFKGDNDSESLGNNLLQD 720

Query: 721 EASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEK 776
           EASE L+SGFDHFR  LVKFFE LNNFAE S++MYAEL  +IQD KS Y+Q KSQ+LEK
Sbjct: 721 EASERLISGFDHFRSRLVKFFENLNNFAEASVKMYAELGKTIQDFKSSYEQWKSQRLEK 778

BLAST of Spg001357 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 3.0e-36
Identity = 186/689 (27.00%), Postives = 291/689 (42.24%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MG + SKVE++  +R C+ER++ +K+A+  R  LA+AHA+Y++SLR T  AL  F Q   
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  PVESPLYTS----TNATPEPLAFTEKSVSQFSFSSPSFSQRVDTV--GNMSPSPSPHTSS 120
            +    +T+    T A P  LA T       S +S S       +     +P P P T S
Sbjct: 61  SLAVSHHTAPVLLTTAAP-ALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQS 120

Query: 121 RFQANHMQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEG 180
                 +  R         K+P      + S     S    +F +P   +   SSA    
Sbjct: 121 HQPPPPVAVRAPRGGPRRLKVPH-----ILSDSSVASPARSSFRKPVVGTPSSSSA---W 180

Query: 181 AWDYFFPSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGG-- 240
            W+ F+P +  +  F D         + E A+ LR  +EE+        + +G LH    
Sbjct: 181 DWENFYPPSPPDSEFFDRRKA-----DLEEANRLRELEEEE--------KARGYLHPHHL 240

Query: 241 EESQNSEDEFDEPASETLVRSFENFNRVHDD--GAENTSPTMHTAKSVASEPELVNQGKN 300
           +E    +D+ DE   E     +E+     DD   +  TS T      + +  E     ++
Sbjct: 241 KEEDEVDDDDDEREEEMHCGGWED----DDDHYASTTTSETRSEEGEMGNRSECGFAARS 300

Query: 301 HSPGLSPLRTTSS--VVPYTSVFGKAPAKEDSIENTAVP--------KDFFSSMKEIESL 360
              G +P    ++   +P      ++ A + S   TA          +     +  IE  
Sbjct: 301 EYGGTAPSEYAAAPLPLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEY 360

Query: 361 FIQASESGKEIPRMLEANKLHIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTA 420
           F++A+E+G  +  +LEA++  +   F   +K    S  L S  S                
Sbjct: 361 FVKAAEAGNGVSELLEASRAQLDRNFRQLKKTVYHSNSLLSSLSS--------------- 420

Query: 421 TKYLTWHRTTSSRSSSSRNPLGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKK 480
               TW         +S+ PL    K       +N  E   M   SH STL+RL AWEKK
Sbjct: 421 ----TW---------TSKPPLAVRYK-----LDTNALEMESMEGKSHGSTLERLLAWEKK 480

Query: 481 LYDEVKASEMVRKEYDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSIS 540
           LY EVKA E V+ E++ K   L+ LE +     K+D+T+A I  L S I V      + S
Sbjct: 481 LYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQSLIIVTSQAATTTS 540

Query: 541 KKIEELRDKELQPQLEELIEGLSRMWEVMFDCHKLQLQIIKAVSN-HGNMKISMQSETRR 600
             I  +RD EL PQL EL   L  MW  M   H++Q +I++ V     N      S+  R
Sbjct: 541 SAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGLVDNSMAESTSDLHR 600

Query: 601 HNTIYLETELASLSSSFTKWIAAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIY 660
             T  LE  +++  S+F + I  Q+ Y++++ GWL   ++L Q  S   + A        
Sbjct: 601 LATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLK--LTLFQVDSNIPQEAYTS----L 624

Query: 661 VPPPIYITCSVWLEKINNLPTKEVVDSIK 669
           +   +   C  W + ++ LP     ++IK
Sbjct: 661 ISRELTTFCDEWKQALDRLPDASASEAIK 624

BLAST of Spg001357 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.4e-32
Identity = 183/714 (25.63%), Postives = 302/714 (42.30%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MG + SK++ + A+R C++R++ +K+A++ R  LA AHA+Y +SLR TG+AL  F   E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
              S    +      P   +E+S ++  F  P FS          PS SP  +S  Q + 
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAK--FVPPRFSPSPAPSSVYPPSTSPSVASSKQPSV 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSA-----------TFQTFERPQTLSFEGSS 180
           M    + +N+  ++ P P +  + S   P S+            + +  +  T S   S 
Sbjct: 121 M---STSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSH 180

Query: 181 APQEGAWDYFFPSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGD------R 240
           A     W+ F+P +  +  F      N    E ++ S  R F +ED  +   +      R
Sbjct: 181 ASSVWNWENFYPPSPPDSEF-----FNRKAQEKKHNSDNR-FNDEDTETVRSEYDFFDTR 240

Query: 241 EEKGSLHGGEESQNSEDEFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPE 300
           ++K        +Q  E+E +    E     +E+    HD  +  +S      +    + E
Sbjct: 241 KQKQKQFESMRNQ-VEEETETEREEVQCSEWED----HDHYSTTSSSDAAEEEEEDDDRE 300

Query: 301 LVNQGKNHSPGLSPLRTTS------SVVPYTSVFGKA-PAKEDSIENTAVP--------- 360
            +++    S   S +R+ S         P   V+G A  +K D  ++  +          
Sbjct: 301 SISEVGTRSEFGSTVRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGD 360

Query: 361 ----------KDFFSSMKEIESLFIQASESGKEIPRMLEANKLHIRPIFPGKEKQSLSST 420
                     +D    +  I+  F +A+ SG+++ +MLE  +  +   F   +K  + S+
Sbjct: 361 IADMKMVVRHRDLKEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSS 420

Query: 421 LLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNPLGANSKEYVEDHSSNLF 480
            L S  S                    TW         +S+ PL     +Y  D ++   
Sbjct: 421 SLLSNLSS-------------------TW---------TSKPPLAV---KYRIDTTALDQ 480

Query: 481 ENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCKILRHLESKEIGQPKIDR 540
            N   +S S  STLDRL AWEKKLY+E+KA E  + E++ K   L+  E K   + K+D+
Sbjct: 481 PN---SSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQEYKGEDEAKLDK 540

Query: 541 TRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIEGLSRMWEVMFDCHKLQL 600
           T+A I  L S I V    + + S  I  LRD +L PQL EL  G   MW+ M   H+ Q 
Sbjct: 541 TKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYMWKSMHQYHETQN 600

Query: 601 QIIKAVSNHGNM--KISMQSETRRHNTIYLETELASLSSSFTKWIAAQKAYLQSIDGWL- 660
            I++ V    N   K    SE  R  T  LE+ ++S  SSF+  I  Q+ ++ S+  W  
Sbjct: 601 SIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSFSSLIKFQRDFIHSVHAWFK 655

Query: 661 LKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPTKEVVDSIK 669
           L  + + Q+ +    +          P   Y  C  W   ++ +P     ++IK
Sbjct: 661 LTLLPVCQEDAANHHKE---------PLDAYAFCDEWKLALDRIPDTVASEAIK 655

BLAST of Spg001357 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-29
Identity = 163/696 (23.42%), Postives = 264/696 (37.93%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MG   S+++  + +  C+ RK+++K  +  R +L+ +HA Y++SLR  G++L  F   E 
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           P+    +   + +P P           S  S + +    T  ++ P P P          
Sbjct: 61  PLHLH-HNPPSPSPPPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPPPPPPPP------- 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
                          P P          PPS+T+                     WD F 
Sbjct: 121 ---------------PPP----------PPSSTWD-------------------FWDPFI 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           P                                                       +SE+
Sbjct: 181 P---------------------------------------------------PPPSSSEE 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           E++E  + T  R+         D A  T+PT  T       P+  +     S       T
Sbjct: 241 EWEEETT-TATRTATG---TGSDAAVTTAPTTAT-------PQASSVVSGFSKDTMTTTT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           T S              E ++  +   KD    +KE++  F++A++SG  +  +LE +  
Sbjct: 301 TGS--------------ELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLEISTS 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHR-TTSSRSSSSRN 420
                F G  K         S + C  +P+               W R    S+ S  RN
Sbjct: 361 ITD--FSGHSKS--GKMYSSSNYECNLNPTSF-------------WTRGFAPSKLSEYRN 420

Query: 421 PLGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKC 480
             G      +               GSH+ST+DRLYAWEKKLY EVK +E ++ +++ K 
Sbjct: 421 AGGVIGGNCI--------------VGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKV 480

Query: 481 KILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELI 540
           + +R LE K     K ++ +  ++ L S++ V    I S S +I +LR+ EL PQL EL+
Sbjct: 481 EQVRRLEMKRAEYVKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELV 528

Query: 541 EGLSRMWEVMFDCHKLQLQIIKAVSNHGNM-KISMQSETRRHNTIYLETELASLSSSFTK 600
           +GL  MW  M++ H++Q  I++ +     +      SE  R +T+ LE E+     SF  
Sbjct: 541 KGLMCMWRSMYESHQVQTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCN 528

Query: 601 WIAAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNL 660
            + AQ+ Y+QS+ GWL   +SL Q S         P +R      IY  C  W   I+ +
Sbjct: 601 LVKAQRDYIQSLTGWLR--LSLFQFSKN-------PLVRSSYESKIYSFCEEWHLAIDRI 528

Query: 661 PTKEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNL 695
           P K   + IK         +  Q     + K  +++
Sbjct: 661 PDKVASEGIKSFLTAVHGIVAQQADEHKQKKRTESM 528

BLAST of Spg001357 vs. ExPASy TrEMBL
Match: A0A1S3BNY8 (uncharacterized protein LOC103491612 OS=Cucumis melo OX=3656 GN=LOC103491612 PE=4 SV=1)

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 678/779 (87.03%), Postives = 718/779 (92.17%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SK+EEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKLEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY ST ATPEPLAFTEKS SQFSFSSPSFS  +DT GN SPSPSP TSSRFQANH
Sbjct: 61  PVESPLYASTFATPEPLAFTEKSASQFSFSSPSFSHHMDTAGNRSPSPSPPTSSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGSFA+KVEEKLPSPVIGTVTSSD PPSA  QT ERPQTLSFEGSSAPQEG WD+FF
Sbjct: 121 MQFRGSFAHKVEEKLPSPVIGTVTSSDTPPSAKPQTSERPQTLSFEGSSAPQEGTWDFFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSF DG+G+NNGG EFENA  +RYFKEEDGN E+GD+E K SLHG EESQNSED
Sbjct: 181 PSNNHEFSFHDGHGVNNGGVEFENAGDVRYFKEEDGNFEYGDKEGKSSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVHDDGA NTSPTMHT KSVASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHDDGASNTSPTMHTVKSVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSS+VP TSVFGKA AK++SIEN AVPKD FSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSLVPLTSVFGKATAKDESIENPAVPKDLFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE  SLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP
Sbjct: 361 HIRPIFPGKENPSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VED+SSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGVNSKEDVEDYSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PKID+TRAVIKDLHSRIRVG+HRIDSISKKIEELRD+ELQPQLEELIE
Sbjct: 481 MLRHLESKEVGLPKIDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDRELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCHK QLQIIKA S HGNMKISM SETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 GLSRMWEVMFDCHKNQLQIIKAASYHGNMKISMHSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
            AQK+YL SIDGWLLKCV+LP   SRGKRRAQ PSI+ + PPPIYITCSVWLEKIN LPT
Sbjct: 601 TAQKSYLHSIDGWLLKCVTLP--PSRGKRRAQTPSIKTFGPPPIYITCSVWLEKINELPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQDEASE 720
           KEVVDSIKDLAAETARFLPHQEKN GKGK AKNLS+LTSFK +ND ES+G NLLQ EASE
Sbjct: 661 KEVVDSIKDLAAETARFLPHQEKNPGKGKVAKNLSVLTSFKGNNDSESIGNNLLQGEASE 720

Query: 721 SLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQSGI 780
           SL+SGFDH RPSLVKFFE+LNNFA+ S++MYAEL  +IQ+ KS Y+Q K+Q++E Q+GI
Sbjct: 721 SLISGFDHLRPSLVKFFEKLNNFADSSVKMYAELGKTIQEFKSHYEQWKAQRMETQAGI 777

BLAST of Spg001357 vs. ExPASy TrEMBL
Match: A0A5A7T9V8 (DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00850 PE=4 SV=1)

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 678/779 (87.03%), Postives = 718/779 (92.17%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SK+EEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKLEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY ST ATPEPLAFTEKS SQFSFSSPSFS  +DT GN SPSPSP TSSRFQANH
Sbjct: 61  PVESPLYASTFATPEPLAFTEKSASQFSFSSPSFSHHMDTAGNRSPSPSPPTSSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGSFA+KVEEKLPSPVIGTVTSSD PPSA  QT ERPQTLSFEGSSAPQEG WD+FF
Sbjct: 121 MQFRGSFAHKVEEKLPSPVIGTVTSSDTPPSAKPQTSERPQTLSFEGSSAPQEGTWDFFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSF DG+G+NNGG EFENA  +RYFKEEDGN E+GD+E K SLHG EESQNSED
Sbjct: 181 PSNNHEFSFHDGHGVNNGGVEFENAGDVRYFKEEDGNFEYGDKEGKSSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVHDDGA NTSPTMHT KSVASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHDDGASNTSPTMHTVKSVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSS+VP TSVFGKA AK++SIEN AVPKD FSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSLVPLTSVFGKATAKDESIENPAVPKDLFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE  SLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP
Sbjct: 361 HIRPIFPGKENPSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VED+SSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGVNSKEDVEDYSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PKID+TRAVIKDLHSRIRVG+HRIDSISKKIEELRD+ELQPQLEELIE
Sbjct: 481 MLRHLESKEVGLPKIDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDRELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCHK QLQIIKA S HGNMKISM SETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 GLSRMWEVMFDCHKNQLQIIKAASYHGNMKISMHSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
            AQK+YL SIDGWLLKCV+LP   SRGKRRAQ PSI+ + PPPIYITCSVWLEKIN LPT
Sbjct: 601 TAQKSYLHSIDGWLLKCVTLP--PSRGKRRAQTPSIKTFGPPPIYITCSVWLEKINELPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQDEASE 720
           KEVVDSIKDLAAETARFLPHQEKN GKGK AKNLS+LTSFK +ND ES+G NLLQ EASE
Sbjct: 661 KEVVDSIKDLAAETARFLPHQEKNPGKGKVAKNLSVLTSFKGNNDSESIGNNLLQGEASE 720

Query: 721 SLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQSGI 780
           SL+SGFDH RPSLVKFFE+LNNFA+ S++MYAEL  +IQ+ KS Y+Q K+Q++E Q+GI
Sbjct: 721 SLISGFDHLRPSLVKFFEKLNNFADSSVKMYAELGKTIQEFKSHYEQWKAQRMETQAGI 777

BLAST of Spg001357 vs. ExPASy TrEMBL
Match: A0A6J1JGH4 (nitrate regulatory gene2 protein OS=Cucurbita maxima OX=3661 GN=LOC111484181 PE=4 SV=1)

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 676/779 (86.78%), Postives = 716/779 (91.91%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SKVEEDKALRLCRERKK VKQALHGRC+LATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKVEEDKALRLCRERKKLVKQALHGRCTLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLY STNATPEPLAFTEKSVSQFSFSSPSFSQRVD V N+SPSPSP TSS+FQANH
Sbjct: 61  PVESPLYHSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDAVSNLSPSPSPPTSSQFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGS ++KVE KLPSPVIGT+TSSDIPPS T QTFERP+ LSFEGSSAPQEGAWDYFF
Sbjct: 121 MQFRGSISHKVEAKLPSPVIGTITSSDIPPSTTRQTFERPRALSFEGSSAPQEGAWDYFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
            S+NHEFSFQDG+G+NNG FEFENA GL+YFKEEDGN E+GD EEKGSLHG EESQNSED
Sbjct: 181 SSDNHEFSFQDGSGVNNGEFEFENAGGLKYFKEEDGNFENGDGEEKGSLHGEEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEP+SETLVRSFENFNRV++DGA NTSPTMHT K+VASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPSSETLVRSFENFNRVYNDGAANTSPTMHTVKTVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSVFGKA AKEDSIENTAVP DFFSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSVVPFTSVFGKATAKEDSIENTAVPMDFFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE QSLSSTLLKSCFSCGDDPSV+REEPVQTATKYLTWHRT SS SS+SRNP
Sbjct: 361 HIRPIFPGKENQSLSSTLLKSCFSCGDDPSVLREEPVQTATKYLTWHRTASSHSSASRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGGNSKEDVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PK+D+TRAVIKDLHSRIRVG+HRIDSISKKIEELRDKELQPQLEELIE
Sbjct: 481 VLRHLESKEVGLPKVDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDKELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
            LSRMWEVMFDCHK QLQ+I+A S HGNM ISMQSETRRHNTIYLETELASLSSSF KWI
Sbjct: 541 ALSRMWEVMFDCHKRQLQVIEAASYHGNMGISMQSETRRHNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
           AAQK YLQ IDGWL  CVSLPQK S+GKRR QAPSIR Y PPPIYITCSVWLEKI++LP 
Sbjct: 601 AAQKLYLQLIDGWLRTCVSLPQK-SKGKRRTQAPSIRTYGPPPIYITCSVWLEKIDDLPM 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKG----AKNLSILTSFKADNDIESMGKNLLQD 720
           KEV+DSIKDLA ET RFLP QEKN GKGKG    AKNLSILTSFK DND ES+G NLLQD
Sbjct: 661 KEVIDSIKDLATETTRFLPRQEKNLGKGKGKGKVAKNLSILTSFKGDNDSESLGNNLLQD 720

Query: 721 EASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEK 776
           EASE L+SGFDHFR  LVKFFE LNNFAE S++MYAEL  +IQD KS Y+Q KSQ+LEK
Sbjct: 721 EASERLISGFDHFRSRLVKFFENLNNFAEASVKMYAELGKTIQDFKSSYEQWKSQRLEK 778

BLAST of Spg001357 vs. ExPASy TrEMBL
Match: A0A0A0KX10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269740 PE=4 SV=1)

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 670/779 (86.01%), Postives = 711/779 (91.27%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SK+EEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG
Sbjct: 1   MGASSSKLEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLYTST ATPEPLAFTEKS SQFSF SPSFS  +D  GN+SPSPSP  SSRFQANH
Sbjct: 61  PVESPLYTSTFATPEPLAFTEKSASQFSFPSPSFSHHMDAAGNLSPSPSPPASSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGSFA+KVEEKLPSPVIGTVTSS+ PPSA  QT ERPQTLSFEGSSAPQEG WD+FF
Sbjct: 121 MQFRGSFAHKVEEKLPSPVIGTVTSSETPPSAKPQTSERPQTLSFEGSSAPQEGTWDFFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSF DG+ +NNGG EFENA G +YFKEEDGN E+GD+E K SLHGGEESQNSED
Sbjct: 181 PSNNHEFSFHDGHEVNNGGVEFENADGAKYFKEEDGNFEYGDKEGKSSLHGGEESQNSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEPASETLVRSFENFNRVHDDGA NTSP MHT KSVASEPELVNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPASETLVRSFENFNRVHDDGASNTSPNMHTVKSVASEPELVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSS+VP T+VFGKA AKE+S+ENTAV KD FSSMKEIE LFI+ASESGKE+PRMLEANKL
Sbjct: 301 TSSLVPLTTVFGKATAKEESVENTAVHKDLFSSMKEIEILFIKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE  SLSSTLLKSCFSCGDDP VVREEPVQTATKYLTWHRT SSRSSSSRNP
Sbjct: 361 HIRPIFPGKENPSLSSTLLKSCFSCGDDPGVVREEPVQTATKYLTWHRTASSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG NSKE VED+SSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK
Sbjct: 421 LGVNSKEDVEDYSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+G PKID+TRAVIKDLHSRIRVG+HRIDSISKKIEELRD+ELQPQLEELIE
Sbjct: 481 MLRHLESKEVGLPKIDKTRAVIKDLHSRIRVGVHRIDSISKKIEELRDRELQPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCHK QLQIIKA S HGNMKISM SETRR+NTIYLETELASLSSSF KWI
Sbjct: 541 GLSRMWEVMFDCHKHQLQIIKAASYHGNMKISMHSETRRYNTIYLETELASLSSSFMKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
            AQK+YL SIDGWLLKCV+LPQ   +GKRR QAP I+ Y PPPIYITCSVWLEKI  LPT
Sbjct: 601 TAQKSYLHSIDGWLLKCVTLPQ--PKGKRRVQAPPIKNYGPPPIYITCSVWLEKIKELPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQDEASE 720
           KEVVDSIKDLAAETARFLPHQEKN  KGK AKNLSILTSFK DND ES+G NLLQ EASE
Sbjct: 661 KEVVDSIKDLAAETARFLPHQEKNPRKGKVAKNLSILTSFKGDNDSESIGNNLLQGEASE 720

Query: 721 SLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQSGI 780
           SL+SGFDH RPSLVKFFE+LNNFA+ S++MY EL  +IQ+ KS Y+Q K+Q+LEKQ+GI
Sbjct: 721 SLISGFDHLRPSLVKFFEKLNNFADSSVKMYEELGKTIQEFKSHYEQWKAQRLEKQAGI 777

BLAST of Spg001357 vs. ExPASy TrEMBL
Match: A0A6J1K1U4 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111490962 PE=4 SV=1)

HSP 1 Score: 1304.7 bits (3375), Expect = 0.0e+00
Identity = 670/777 (86.23%), Postives = 716/777 (92.15%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS+SKVEEDKALRLC  RKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFV PEG
Sbjct: 1   MGASSSKVEEDKALRLCHIRKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVHPEG 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           PVESPLYTSTNATPEPLAF EKS+SQFSFSSPSFSQRVD +GN SPSPSP TSSRFQANH
Sbjct: 61  PVESPLYTSTNATPEPLAFAEKSMSQFSFSSPSFSQRVD-IGNFSPSPSPPTSSRFQANH 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDYFF 180
           MQFRGSF+N+VEEKLP+PVIGTVTSSD+ PS      + PQT SFEGSSAPQ+G WDYFF
Sbjct: 121 MQFRGSFSNRVEEKLPTPVIGTVTSSDVRPS------DAPQTRSFEGSSAPQDGTWDYFF 180

Query: 181 PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQNSED 240
           PSNNHEFSFQDGNGMNNGGFEFEN  GL++FKEEDGNSE+ +REEKGSLH GEESQ+SED
Sbjct: 181 PSNNHEFSFQDGNGMNNGGFEFENTGGLKHFKEEDGNSEYREREEKGSLHVGEESQHSED 240

Query: 241 EFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLSPLRT 300
           EFDEP+SETLVRSFENFNRVH+DGA NTSPTMHT KSVASEPE VNQGKNHSPGLSPLRT
Sbjct: 241 EFDEPSSETLVRSFENFNRVHNDGAVNTSPTMHTVKSVASEPEFVNQGKNHSPGLSPLRT 300

Query: 301 TSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRMLEANKL 360
           TSSVVP+TSVFGKA AKED IENTAVPKD FSSMKEIESLF +ASESGKE+PRMLEANKL
Sbjct: 301 TSSVVPFTSVFGKATAKEDGIENTAVPKDLFSSMKEIESLFTKASESGKEVPRMLEANKL 360

Query: 361 HIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRSSSSRNP 420
           HIRPIFPGKE Q LSS  LKSCFSCGDDPSVVREEPVQTATKYLTWHRT SSRSSSSRNP
Sbjct: 361 HIRPIFPGKENQPLSSRFLKSCFSCGDDPSVVREEPVQTATKYLTWHRTASSRSSSSRNP 420

Query: 421 LGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKEYDLKCK 480
           LG +SKE VEDHSSNLF NFCMNSGSHASTLDRLYAWEKKL+DEVKA+EMVRKEYDLKCK
Sbjct: 421 LGGHSKEDVEDHSSNLFGNFCMNSGSHASTLDRLYAWEKKLHDEVKANEMVRKEYDLKCK 480

Query: 481 ILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQLEELIE 540
           +LRHLESKE+GQPKID+TRAVIKDLHSRIRVGLHRIDSISKKIEELRD EL PQLEELIE
Sbjct: 481 MLRHLESKEVGQPKIDKTRAVIKDLHSRIRVGLHRIDSISKKIEELRDNELHPQLEELIE 540

Query: 541 GLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSSSFTKWI 600
           GLSRMWEVMFDCHK QLQIIKAVS HG MKISMQSET+RHNTIYLETELASLSSSFTKWI
Sbjct: 541 GLSRMWEVMFDCHKNQLQIIKAVSYHGKMKISMQSETQRHNTIYLETELASLSSSFTKWI 600

Query: 601 AAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEKINNLPT 660
           AAQK+YLQSI+ WLL CVS+PQKSSRGK+R Q PSIR Y PPPIY+TC VWLEK  +LPT
Sbjct: 601 AAQKSYLQSINEWLLTCVSIPQKSSRGKKRPQPPSIRNYGPPPIYVTCGVWLEKSKDLPT 660

Query: 661 KEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGKNLLQD-EAS 720
           K+VVDSIKDLAAETARFLPHQE+NQ  GKGAK+LSILTSFKA+ND ESMG NLLQD EAS
Sbjct: 661 KDVVDSIKDLAAETARFLPHQERNQVNGKGAKHLSILTSFKAENDSESMGNNLLQDEEAS 720

Query: 721 ESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGKSQKLEKQ 777
            SLVSGFD+F  SL+KFFE LNNFAECS++MY +L NSI+DAK++Y+QGKSQKLEK+
Sbjct: 721 RSLVSGFDNFGASLIKFFENLNNFAECSMKMYGDLGNSIKDAKNRYEQGKSQKLEKE 770

BLAST of Spg001357 vs. TAIR 10
Match: AT2G27090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 757.7 bits (1955), Expect = 9.3e-219
Identity = 443/776 (57.09%), Postives = 534/776 (68.81%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MGAS S+++EDKAL+LCRERKKFV+QAL GRC LA AH  Y+QSL+ TGTAL+ F + E 
Sbjct: 1   MGASTSRIDEDKALQLCRERKKFVQQALDGRCLLAAAHVSYVQSLKSTGTALRKFSETEV 60

Query: 61  PVESPLYTSTNATPE-PLAFTEKSVSQFSFSSPSFS-QRVDTVGNMSPSPSPHTSSRFQA 120
           PVES LYTST+ATPE PLA  EKSVS  S+S P  S    DT    SP PSP ++S FQ 
Sbjct: 61  PVESSLYTSTSATPEQPLALIEKSVSHLSYSPPPASHSHHDT---YSPPPSPPSTSPFQV 120

Query: 121 NHMQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAWDY 180
           NHM+F+G  + KVEEK P  +I TVTSS IPPS + +  E   T   E SS P E  WDY
Sbjct: 121 NHMKFKGFSSKKVEEKPPVSIIATVTSSSIPPSRSIEKME--STPFEESSSMPPEAPWDY 180

Query: 181 F---FPSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEES 240
           F    P +N   S   GNG  +           R  KEEDG  E  D  E  S    EES
Sbjct: 181 FGLSHPIDNQFSSSHVGNGHVS-----------RSVKEEDGTPEEEDDGEDFSFQEREES 240

Query: 241 QNS-EDEFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPG 300
           ++S +DEFDEP S+TLVRSFENFNRV  D   +T P     +S +S+ E     K+ +P 
Sbjct: 241 RDSDDDEFDEPTSDTLVRSFENFNRVRRD--HSTLPQREGVESDSSDAE-----KSKTPE 300

Query: 301 LSPLRTTSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASESGKEIPRM 360
           LSP  T     P      K P K D  EN   P+DF SSMKEIE LF++ASE+GKE+PRM
Sbjct: 301 LSPPVTPLVATP----VNKTPNKGDHTENKLPPRDFLSSMKEIELLFVKASETGKEVPRM 360

Query: 361 LEANKLHIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTWHRTTSSRS 420
           LEANKLH RPI P KE  S +S+L K+C SCG+DP  V EEP Q + KYLTWHRT SSRS
Sbjct: 361 LEANKLHFRPIVPSKESGSGASSLFKTCLSCGEDPKDVPEEPAQNSVKYLTWHRTESSRS 420

Query: 421 SSSRNPLGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVRKE 480
           SSSRNPLG  + + VE+ +SNLFEN CM +GSHASTLDRLYAWE+KLYDEVK S+ VR+E
Sbjct: 421 SSSRNPLGGMNSDDVEELNSNLFENICMIAGSHASTLDRLYAWERKLYDEVKGSQTVRRE 480

Query: 481 YDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQPQ 540
           YD KC+ILR LES+  G  +ID+TRAV+KDLHSRIRV +HRIDSIS++IEELRD ELQPQ
Sbjct: 481 YDEKCRILRELESEGKGSQRIDKTRAVVKDLHSRIRVAIHRIDSISRRIEELRDNELQPQ 540

Query: 541 LEELIEGLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQSETRRHNTIYLETELASLSS 600
           LEELIEGLSRMWEVM +CHK+Q Q+IKA    GN+K++MQSE  R  T +LE EL +L+S
Sbjct: 541 LEELIEGLSRMWEVMLECHKVQFQLIKACYRGGNIKLNMQSELHRQVTSHLEDELCALAS 600

Query: 601 SFTKWIAAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVWLEK 660
           SFTKWI  QK+Y+Q+I+ WL+KCV+LPQ+S R KRRA  PS+R Y  PPIY TC +WLEK
Sbjct: 601 SFTKWITGQKSYIQAINEWLVKCVALPQRSKR-KRRAPQPSLRNY-GPPIYATCGIWLEK 660

Query: 661 INNLPTKEVVDSIKDLAAETARFLPHQEKNQ-GKGKGAKNLSILTSFKADNDIESMGKNL 720
           +  LPTKEV  SIK LA++ ARFLP QEKN+  K +  +N + LT+            ++
Sbjct: 661 LEVLPTKEVSGSIKALASDVARFLPRQEKNRTKKHRSGENKNDLTA------------HM 720

Query: 721 LQDEASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKYDQGK 770
           LQDE  E    GFD FR SL  F  +LN FAE S++MY EL+  I  AK+ Y+Q K
Sbjct: 721 LQDETLEDCGPGFDRFRTSLEGFVGQLNQFAESSVKMYEELKEGIHGAKNNYEQLK 735

BLAST of Spg001357 vs. TAIR 10
Match: AT4G39790.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 316.6 bits (810), Expect = 5.5e-86
Identity = 264/774 (34.11%), Postives = 368/774 (47.55%), Query Frame = 0

Query: 1   MGASNSKV---EEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQ 60
           MG SNSK    ++++ L LC+ERK+FVKQA+  RC+LA AH  YI+SLR  G  L+ + +
Sbjct: 9   MGCSNSKASMNKKNEPLHLCKERKRFVKQAMDSRCALAAAHVSYIRSLRNIGACLRQYAE 68

Query: 61  PEGPVE-SPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRF 120
            E   E SP  T+T    EP    EKS S  S S P  S  VD+  + + +P+P+    F
Sbjct: 69  AETAHESSPSLTAT----EP----EKSPSHNS-SYPDDS--VDSPLSHNSNPNPNPKPLF 128

Query: 121 QANHMQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEGSSAPQEGAW 180
             ++M+       +      +  I  ++  D     T   F  P          P+  +W
Sbjct: 129 NLSYMK------TETASSTVTFTINPLSDGDDDLEVTMPAFSPPPP---PRPRRPETSSW 188

Query: 181 DYFFPSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSLHGGEESQ 240
           DYF   ++ +             F F    GL    E D         E  +   G E  
Sbjct: 189 DYFDTCDDFD------------SFRF---VGLSEQTEID--------SECDAAVIGLEKI 248

Query: 241 NSEDEFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAKSVASEPELVNQGKNHSPGLS 300
            S+    +  SETL               +++  T    +S     E             
Sbjct: 249 TSQGNVAKSGSETL--------------QDSSFKTKQRKQSCEDNDE------------- 308

Query: 301 PLRTTSSVVPYTSVFGKAPAKEDSIE-NTAVPKDFFSSMKEIESLFIQASESGKEIPRML 360
                               +ED  E  T   KDF SSMK+IE  F +ASESG+E+ RML
Sbjct: 309 --------------------REDPSEFITHRAKDFVSSMKDIEHKFFRASESGREVSRML 368

Query: 361 EANKLHIR-PIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPV-QTATKYLTWHRTTSSR 420
           E NK+ +      GK         LK     G   S V +EP+    TK + W RT+SSR
Sbjct: 369 EVNKIRVGFADMTGKGNSIAFLAALKRACCRGKSYSPVSQEPLSHQVTKVIVWKRTSSSR 428

Query: 421 SSSSRNPLGANSKEYVEDHS-SNLFENFCMNSGSHASTLDRLYAWEKKLYDEVKASEMVR 480
           SS+SRNPL   SKE  +D S S+  E FCM SGSH+S+LDRLYAWE+KLYDEVKASEM+R
Sbjct: 429 SSTSRNPLIQTSKEDHDDESGSDFIEEFCMISGSHSSSLDRLYAWERKLYDEVKASEMIR 488

Query: 481 KEYDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEELRDKELQ 540
           KEYD KC+ LR+  +K+     +D+TRA  KDLHSRIRV +  ++SISK+IE +RD EL 
Sbjct: 489 KEYDRKCEQLRNQFAKDHSAKSMDKTRAAAKDLHSRIRVAIQSVESISKRIERIRDDELH 548

Query: 541 PQLEELIEGLSRMWEVMFDCHKLQ-LQIIKAVSNHGNMKISMQSETRRHNTIYLETELAS 600
           PQL E ++GL RMW+ M +CH  Q + I  A     + K + +S  +R     L  E   
Sbjct: 549 PQLLEFLQGLIRMWKAMLECHHTQYITISLAYHCRHSSKTAHESVLKRRILAELLEETEC 608

Query: 601 LSSSFTKWIAAQKAYLQSIDGWLLKCVSLPQKSSRGKRRAQAPSIRIYVPPPIYITCSVW 660
              SF   + +  +Y+++++GWL  CV LPQ+ S   RR  +P  R  + PPI++ C  W
Sbjct: 609 FGLSFVDLVHSMASYVEALNGWLHNCVLLPQERSTRNRRPWSP--RRVLAPPIFVLCRDW 654

Query: 661 LEKINNLPTKEVVDSIKDLAAETARFLPHQEKNQGKGKGAKNLSILTSFKADNDIESMGK 720
              I  LP+ E+  SIK  +                                 D+E +G 
Sbjct: 669 SAGIKTLPSDELSGSIKGFSL--------------------------------DMEMLG- 654

Query: 721 NLLQDEASESLVSGFDHFRPSLVKFFERLNNFAECSLEMYAELRNSIQDAKSKY 766
              +++    LVS       SL K  ERL  F+E SL+MY +++   + A+  Y
Sbjct: 729 ---EEKGGSLLVSDLSSVHSSLAKLLERLKKFSEASLKMYEDVKVKSEAARVAY 654

BLAST of Spg001357 vs. TAIR 10
Match: AT4G30130.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 230.3 bits (586), Expect = 5.2e-60
Identity = 207/715 (28.95%), Postives = 330/715 (46.15%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MG S+SK ++D+A+++C++RK+F+KQA+  R   A+ H  YIQSLR    AL+ +++ + 
Sbjct: 1   MGCSHSKFDDDEAVQICKDRKRFIKQAVEHRTGFASGHIAYIQSLRKVSDALREYIEGDE 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPHTSSRFQANH 120
           P E  L T       P+     S      S PS   + +    ++ +           ++
Sbjct: 61  PHEFMLDTCVT----PVKRMSSSGGFIEISPPSKMVQSEAESKLNVN-----------SY 120

Query: 121 MQFRGSFANKVEEKLPSPVIGTVTSSDIPPSATFQTFERPQTLSFEG-------SSAPQE 180
           +   GS   +VEEK P     +  +  +        F     ++  G         +PQ 
Sbjct: 121 LMASGSRPVRVEEKPPR----SPETFQVETYGADSFFGMNMNMNSPGLGSHNIPPPSPQN 180

Query: 181 GAWDYFF----PSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEEDGNSEHGDREEKGSL 240
             WD+F+      + + +S+ + NGM+      ++   LR  +EE+G     D EE    
Sbjct: 181 SQWDFFWNPFSALDQYGYSYDNQNGMD------DDMRRLRRVREEEGIP---DLEE---- 240

Query: 241 HGGEESQNSEDEFDEPASETLVRSFENFNRVHDDGAENTSPTM-HTAKSVASEPELVNQG 300
              +E    ED  +  A+E     F       +D  E+ +     +   + +E +    G
Sbjct: 241 ---DEYVKFEDHHNMKATE----DFNGGKMYQEDKVEHVNEEFTDSGCKIENESDKNCNG 300

Query: 301 KNHSPGLSPLR--TTSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQASE 360
                 L   R  TT  VV  TS  GK      ++     P      +K++E  F     
Sbjct: 301 TQERRSLEVSRGGTTGHVVGVTSDDGKGETPGFTVYLNRRPTSMAEVIKDLEDQFAIICT 360

Query: 361 SGKEIPRMLEANKLHIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLTW 420
           +GKE+  +LEA+++           Q  SS  L          ++    PV        +
Sbjct: 361 AGKEVSGLLEASRV-----------QYTSSNELS---------AMTMLNPV------ALF 420

Query: 421 HRTTSSRSSSSRNPL-----GANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKL 480
               SSRSSSS   L     G+ + E+  + SS   E  CM SGSH STLDRLYAWEKKL
Sbjct: 421 RSGGSSRSSSSSRFLISSSGGSRASEF--ESSSEFSEESCMLSGSHQSTLDRLYAWEKKL 480

Query: 481 YDEVKASEMVRKEYDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISK 540
           YDEVK+ + +R  Y+ KC +LR+ + K      +D+TRA I+DLH++I+V +H I+SIS+
Sbjct: 481 YDEVKSGDRIRIAYEKKCLVLRNQDVKGADSSAVDKTRATIRDLHTQIKVSIHSIESISE 540

Query: 541 KIEELRDKELQPQLEELIEGLSRMWEVMFDCHKLQ------LQIIKAVSNHGNMKISMQ- 600
           +IE LRD+EL PQL EL++GL++MW+VM +CH++Q       +++ A +     K   Q 
Sbjct: 541 RIETLRDQELLPQLLELVQGLAQMWKVMAECHQIQKRTLDEAKLLLATTPSNRHKKQQQT 600

Query: 601 ------SETRRHNTIYLETELASLSSSFTKWIAAQKAYLQSIDGWLLKCVSLPQKSSRGK 660
                 S+    + ++L  +L +  + F  WI +Q++Y+ S+ GWLL+C        +  
Sbjct: 601 SLPEINSQRLARSALHLVVQLRNWRACFQAWITSQRSYILSLTGWLLRCFRCDPDPEKVT 640

Query: 661 RRAQAPSIRIYVPPPIYITCSVWLEKINNLPTKEVVDSIKDLAAETARFLPHQEK 684
             +         P PIY  C  W   +N L  K V+D +   A+        Q K
Sbjct: 661 LTS--------CPHPIYEVCIQWSRLLNGLNEKPVLDKLDFFASGMGAIYARQLK 640

BLAST of Spg001357 vs. TAIR 10
Match: AT2G19090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 196.4 bits (498), Expect = 8.3e-50
Identity = 212/836 (25.36%), Postives = 358/836 (42.82%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFV---Q 60
           MG S+SK+++++A+++C++RK+F+KQA+  R   A+ H  YI SLR    AL  F+    
Sbjct: 1   MGCSHSKLDDEEAVQICKDRKRFIKQAIEHRTKFASGHIAYIHSLRKVSDALHDFILQGD 60

Query: 61  PEGPVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSP------H 120
                   L   +  TP       +  S  S S    S        +SPS  P       
Sbjct: 61  NNNEFVPTLCQDSFVTPVKRMPQRRRRSSRSNSGEFIS--------ISPSSIPPKMIQGR 120

Query: 121 TSSRFQANHMQFRGSFANKVEEKLPSPVIGTVTSSDIPPS-------------------- 180
             S  +AN++    S   +VE++ P        S   PPS                    
Sbjct: 121 PRSNVKANYLMANRSRPVRVEQRSPETFRVETFS---PPSSSNQYGESDGFFGMNMNMNT 180

Query: 181 --ATFQTFERP-----QTLSFEG--SSAPQEGAWDYFF-PSNNHEF----SFQDGNGMNN 240
             +T  +F  P     Q LS       +PQ   WD+F+ P ++ ++    S+  G+  + 
Sbjct: 181 SASTSSSFWNPLSSPEQRLSSHNIPPPSPQNSQWDFFWNPFSSLDYYGYNSYDRGSVDSR 240

Query: 241 GGFEFENASGLRYFKEEDG------------------------------NSEHGDREEKG 300
            G + +   GLR  +EE+G                                E+  + +K 
Sbjct: 241 SGID-DEIRGLRRVREEEGIPDLEEDDEPHKPEPVVNVRFQNHNPNPKATEENRGKVDKS 300

Query: 301 SLHGGEESQNSEDEFDEPASETLVRSFENFNRVHDDGAEN--TSPTMHTAKSVASEPELV 360
             +   + ++ +++ DE   E      ++     ++G E     PT    K+        
Sbjct: 301 CCNEEVKVEDVDEDEDEDGDEDDDEFTDSGCETENEGDEKCVAPPTQEQRKAEVCRG--- 360

Query: 361 NQGKNHSPGLSPLRTTSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQAS 420
            +   +  G+  ++   +VV    V   A     ++     P      +K++E  F    
Sbjct: 361 GEATGNVVGVGKVQEMKNVV---GVRDDAKTTGFTVYVNRRPTSMAEVIKDLEDQFTTIC 420

Query: 421 ESGKEIPRMLEANKLHIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLT 480
           ++ KE+  +LEA +      F                           +   +     + 
Sbjct: 421 DAAKEVSGLLEAGRAQYTSSF--------------------------NDHSARKMLNPVA 480

Query: 481 WHRTTSSRSSSSRNPL--GANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYD 540
             R+ SSRSSSSR  +     S+E   +  S++ +  CM SGSH +TLDRL+AWEKKLYD
Sbjct: 481 LFRSGSSRSSSSRFLITSSGGSRESGSESRSDVSDESCMISGSHQTTLDRLFAWEKKLYD 540

Query: 541 EVKASEMVRKEYDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKI 600
           EV++ E VR+ Y+ KC  LR+ + K      +D+TRA I+DL ++I+V +H I+SISK+I
Sbjct: 541 EVRSGERVRRAYEKKCMQLRNQDVKGDDPLAVDKTRATIRDLDTQIKVSIHSIESISKRI 600

Query: 601 EELRDKELQPQLEELIEGLSRMWEVMFDCHKLQLQIIKAV----------SNHGN----- 660
           E LRD+EL PQL EL+EGL+RMW+VM + H++Q + +               H       
Sbjct: 601 ETLRDQELLPQLLELVEGLTRMWKVMAESHQIQKRTLDEAKLLLAGTPVSKRHKKRQPPI 660

Query: 661 MKISMQSETRRHNTIYLETELASLSSSFTKWIAAQKAYLQSIDGWLLKCVSLPQKSSRGK 720
           M  ++ S+    + + LE +L +  + F  WI +Q++Y++++ GWLL+C        + +
Sbjct: 661 MPEAINSQRLAQSALNLEAQLRNWRTCFEFWITSQRSYMKALSGWLLRCFRCDPDPEKVR 720

Query: 721 RRAQAPSIRIYVPPPIYITCSVWLEKINNLPTKEVVDSIKDLAAETARFLPHQEKN---- 737
             +           PIY  C  W   +N+L  K V+D ++  A+        Q +     
Sbjct: 721 LSS--------CLHPIYRVCIQWSRLLNSLNEKPVLDKLEFFASGMGSIYARQVREDPNW 780

BLAST of Spg001357 vs. TAIR 10
Match: AT2G17110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 180.3 bits (456), Expect = 6.2e-45
Identity = 191/787 (24.27%), Postives = 337/787 (42.82%), Query Frame = 0

Query: 1   MGASNSKVEEDKALRLCRERKKFVKQALHGRCSLATAHAEYIQSLRCTGTALKIFVQPEG 60
           MG S SK+++  A+ LCR+R  F++ A+H R +L+ AH  Y QSL+    +L  F+    
Sbjct: 1   MGCSTSKLDDLPAVALCRDRCSFLEAAIHQRYALSEAHVSYTQSLKAISHSLHQFIN--- 60

Query: 61  PVESPLYTSTNATPEPLAFTEKSVSQFSFSSPSFSQRVDTVGNMSPSPSPH--------T 120
                 Y  +++  +     +       F S S S   D + ++  SP  H         
Sbjct: 61  --HHHRYNDSDSPKKAKPKMDSGSGHLDFDSDSDSDDDDDIDSLHSSPLHHHLEDDDSNP 120

Query: 121 SSRFQANHMQFRGSFANKVEEKLPSPV----IGTVTSSDIPPSATFQTFERPQTLSFEGS 180
            S    N+M+      + V E+ PS       G  +SS       +        L     
Sbjct: 121 KSYLHMNYMKNSYMPPSLVYEQRPSSPQRVHFGESSSSSTSEYNPYLNSNYGSKLPPPPP 180

Query: 181 SAPQEGAWDYFFPSNNHEFSFQDGNGMNNGGFEFENASGLRYFKEED--GNSEHGDREEK 240
           S P+E  WD+  P + +   +           E  +  G+   +E+D      HG ++  
Sbjct: 181 SPPREKVWDFLDPFDTYYTPYTPSRDTR----ELRDELGVPDLEEDDVVVKEVHGKQKFV 240

Query: 241 GSLHGGEESQNSEDEFDEPASETLVRSFENFNRVHDDGAENTSPTMHTAK-SVASEPELV 300
            ++             +EP   +   +              T P++   K  +  E  +V
Sbjct: 241 AAV-----------SVEEPLGNSGASTSGGGGGGGKASLYQTRPSVSVEKEEMEHEVHIV 300

Query: 301 NQGKNHSPGLSPLRTTSSVVPYTSVFGKAPAKEDSIENTAVPKDFFSSMKEIESLFIQAS 360
            +      G   +R + + V          A+        VP+      KEIE+ F++A+
Sbjct: 301 EKKIVEDSGGDEVRKSKAAV----------ARGGGGVRRGVPE----VAKEIEAQFLRAA 360

Query: 361 ESGKEIPRMLEANKLHIRPIFPGKEKQSLSSTLLKSCFSCGDDPSVVREEPVQTATKYLT 420
           ESG EI  MLE           GK      +   K  +     PSVV             
Sbjct: 361 ESGNEIAVMLEV----------GKHPYGRKNVSSKKLYEGTPSPSVV------------- 420

Query: 421 WHRTTSSRSSSSRNPLGANSKEYVEDHSSNLFENFCMNSGSHASTLDRLYAWEKKLYDEV 480
               +S++SS+S+      S        +++     + S + +STL +L+ WEKKLYDEV
Sbjct: 421 ----SSAQSSTSKKAKAEASSSVTAPTYADIEAELALKSRNLSSTLHKLHLWEKKLYDEV 480

Query: 481 KASEMVRKEYDLKCKILRHLESKEIGQPKIDRTRAVIKDLHSRIRVGLHRIDSISKKIEE 540
           KA E +R  ++ K + L+ ++ +     K+D TR +++ L ++IR+ +  +D IS  I +
Sbjct: 481 KAEEKMRVNHEKKLRKLKRMDERGAENQKVDSTRKLVRSLSTKIRIAIQVVDKISVTINK 540

Query: 541 LRDKELQPQLEELIEGLSRMWEVMFDCHKLQLQIIKAVSNHGNMKISMQ-SETRRHNTIY 600
           +RD+EL  QL ELI+GLS+MW+ M +CHK Q + IK     G ++ S          T  
Sbjct: 541 IRDEELWLQLNELIQGLSKMWKSMLECHKSQCEAIKEARGLGPIRASKNFGGEHLEVTRT 600

Query: 601 LETELASLSSSFTKWIAAQKAYLQSIDGWLLKCVSL-PQKSSRGKRRAQAPSIRIYVPPP 660
           L  EL +    F+ W++AQK +++ ++ WL+KC+   P+++  G        I     P 
Sbjct: 601 LGYELINWIVGFSSWVSAQKGFVRELNSWLMKCLFYEPEETPDGIVPFSPGRIG---APM 660

Query: 661 IYITCSVWLEKINNLPTKEVVDSIKDLAAETARF-----LPHQEKNQGKGKGAKNLSILT 720
           I++ C+ W + ++ +  KEV+++I+              L  +E+  G G   +N+    
Sbjct: 661 IFVICNQWEQALDRISEKEVIEAIRRFTTSVLHLWEQDRLATRERIIGHG-DPRNMD-RE 720

Query: 721 SFKADNDIESMG-KNLLQDEASESLV----SGFDHFRPSLVKFFERLNNFAECSLEMYAE 761
             +   +I+ +  K +L     +++V    +  +  + SL + FE +  F E SL+ Y +
Sbjct: 721 EQRIQKEIQELEMKMVLVGPGEDNIVYQSDTSNESLQGSLQRIFEAMERFTEESLKAYVD 721