Spg001335 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg001335
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Lysine--tRNA ligase
Location	scaffold8: 48169072 .. 48174010 (+)
RNA-Seq Expression	Spg001335
Synteny	Spg001335

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGCAATGGGCACATGCAATCAGAGTGGATAAAGGGCTCCTTTGGGTTACGCTTCTTCTGAGTTCTGATACACACCACACCAAGACGAAGTGAGAGGCGAGAGTCAGTCTAATGGAAGCCTTGAAGCTCTACGGGACTATTTGCTCCCAGCCATTCTTCAAGCATCTCATTCGCTTAGCTTCTTCCTCTTCTACAATCACCGCCCTTGCTCCCAAATCTTCTCGTTCCACCATTTTCCTCCGCCGCTGTTCTTCCACTGCAACCTCCACTGCTGGAGACGGAAAGGTCAAGCTCTCCGGGCGCAGCCGCCGTGCTTCCTCCTCGACTACATCGTCCCCTTCTTCCACGTCTGATAGGGACGCGGTTCGTGCAATTCGCTTAAAGAAGGTTATTCTCTAACCACTTACAAATGCCCCTCGGCTCTTTTCTTTTTCTACTTGCTTGATTGTGTTGGACATTCATGTTGTTGTGCTAGGTTGAAGAATTGAGGAGCAAGGGCTTGGAACCTTATGCGTATACGTGGGACAGGACTCATACTGCCAGTCAGCTGCAAGAGTTGTATAAGCATTTAGGCAATGGTGAAGAGTCTAATAGCGACACTGATCGTGTGTCAATAGCTGGAAGAATTGTGGCTCGCCGAGCGTTTGGAAAGCTTGCATTTCTGACATTAAGAGATGGTTCTGGTACAATTCAGGTTCGTACACTCGCTTGCTTCAATGGACTAACATACACGCATGCACGCTATCACTTCGTCAATTCTCTCCTAGGATGGGTGAAATTTTATGCAGTGCATATAATATTATACTTCTTCGAGGTGGCAAAGTATACATTTTCAAGAAGAGGAAAACGATGGCGGTGAACATGAGTGGGAGATCACTTGACGTTTTGTCTGTTTTATTTTGTTCGCTCGTTATATCCATGGGAATGACGTCTTTTTAGTTAGTTAGTTTTGAGTTATATCCTTTATGATCTATTGTACAAGGTGCTGGTTTGAAGAGTCCTCTACTTCTGCTCTTCTACGCATATACCACATAACCGACATAATTGATTGAACTTTTGGTTTCAGCTTTACTGTGAGAAGGAAAGGCTCATAGATGATCAGTTCGATCAGCTAAAGAATCTTGTTGATATTGGTGATATTCTTGGTGTGCGAGGTTCAATTAAGCGAACTGAGAAAGGTGAGTTCTCATGCTGTTGTTTCTTAATGGTGAGATCACATTTTCCTATTGGCATGAAAAAGTTTCTGCAGTTAACTTCTGGAAGCTAGAGTCTTAGAAGTTAAATACTCTTCGGATGTAGCTATCACAGATAAGAACTAAATTTTCATATACATCTAAATGAAATTAGGGGAGAACCTCAAATATTATTCTTATCTCAATCTTATTTTCCGTACTATACTCGATTGTCCAATGTTCCATATGAAAGTGCAACCAAATTTAATCGTATTTTCTGTTTTTCAAGAAAGATTAAAATAGATAGATTTTCAAGTTTGACATCTAAGTATTTGAGGCATGTAATAACTATTTTCCTCTACACTGACCTTTAATTGAGATTGGATGGTGAACTGTTTGTTATATTGTTTTGTCATTTCTTCAGAGCATAGGAATAAGAAAAAATCTCTTTATGTACATTTTCTTTACCCATTCTAATGGGCGAGTGGTATTCTGAGACCAGATTAGGAGTTTTTTTTGTATTGTACTTGGAACAATAAGAGAAAGTATAAGCATGACTTTTTTCATATTCTTTTTTTAAGCTAGAAGTTATCATGAAAATGAATAATTCAGGTATGACATACAAATTTTGAATTCTAAAGTTTGTTATATATATAAATACACACACATTAAAAATTATTTACCCATGCATTAATCCTCTATATTCCTTTCTGCATATGTTAAGCCTGGAGGACTTATGTCTACGTGGATCTGAACTGTATTTTCCCCTTATTAAGCATTTGAATTTGTTGTGATTTATTTTTTTTGTCTTTCATTTCCTTCAGGTGAACTTTCTGTTTGTTTGAATTCTTTTGAAATCCTGACAAAGTCCCTACTTCCACTTCCTGACAAATATCATGGTTTAACTGATGTTGATAAGCGCTACCGCCAAAGGTAATTTTTGGTCCTTTTATTTTCTGTTCTTTTTCAAAAAAAATCTAATTTTTGGGTGAGCATTTGTCTATGAATGTCTTTTGTTATAAGAGTTTCGAATATCACATTTTGATACTGTAATCAATTGTCGGGGGCTCATATCCGACATGATATTATTTCTTTCCTGTGGAAGAAAATGCATACAATTGAATGGGAACATTTATTTCTATTTCAATTGGAATACAATTGTTTTTTAGGGGTCTTCTTTTTTGTGAAAACCTTCTGATGATTATTCAGGCCTTTCCCTTTCTTAAGAAAATCTTAGAATGTCTTTGAAAATCAAGTTGGCTTCGAGGGATTTCTCATCCACTCCCTTTGTGTTCCTCTCCTTATATATGTGTGTGTATATATATTTGATATTTGAGTGTTCAGGGTAGCTCAAGCTACCTCAACTATTCTCACAGTTTTTTAATATATTGTTTGTTATCTATGAAAAATTATTTACCTTTTGTTTCACTATCTAGATATCTTTTCAACCGTATGAAGACATTTCACTTCAAATGAATAACATGCATTTGAAATACACTTCAGCTCAAAAATTCATTTTCAAAATTGATGTCATCTTGATGTATTTTATTTTCAGGTATGTAGATATGATTGCAAATCCTGAAGTAGCTGATACATTCCGGAAAAGAGCAAAGGTGATGTAGGCTTAGCTATTTCTTATTTGTAACTTGTAAGCATAATTATATTATTAAGCCCCTCGTTGATTTTCGATCCGTTCCCGTACCTACAAATTTCTCTCATATTTCCTTGTTTTTCAAAAATGTGTCTCCAAATAATCACAAGGAACCTAAAATTTTGTAATTACTCTCCTATCTATATCTCAACTCTAGTTCAAGCATTTTTATTTTAACTTTTGAGGAGTTGCCTTCTCTCTTGTAAATTTCATTGTTGGTTTCTTATTAAAAAAGAAAAGAAGGGAAAAAGAAAAACAATGTTGCTAGGTTCAAACCTCTGCCCATATATGTGATGTAATATTTTCAAAAAGTAATCTTAAGTTCTCTAATACTTTGACCTCCTTGTTACCCTTATTTATATAGATAATATCAGAGATTCGCAAGACCGTGGAATCATTAGGATTTGTTGAAGTTGAGACTCCAGTCCTACAGGTTCGTGAATATTCTCATGGCTTCATTTGAATATTATATCTAATTTAGTAGACTTTTTAAAATTTTCTAATGAAGATTAATTTACACGTTCAAAATCTGAGGGTCAATTAGAGTTAATTAATCATCTTAATTCCCTTGATTTTGGCTCCAAATTTCTCTCTCTCTTTTTTTACACCGGCCATCTGAGATCTCCTTTGTTTGCTTGTTTACCTCCATGAACTATTTTGTCTTTAGTTGATGAGTTTCATATTGTGTACGTACACACATGCAGGAACCAATTTTCATTAAGATTCATATTCATGTATTTTTCTTTATGATCCAAGTAGTTTTGGGTTTATTGACTTGGACTTTGGCTCCTTTTGTCTGTATTACCTTGAATTTGTTCTTTCATTCTTCTTATGGAAAGTTTCTTTTCCCACTCAAAATATAAAAGAAGTAAATTTGACTCTATTGACTTGTAGATGCCACTTGACTGTTACTCATTTTGGACGTTGACTTTTTTTGTAAGCATAACAATCTCTGTCCCTCGCTCTCTTTCTCACTCCCTTTCTCCCTCTAGTAATTGACCACTTCCTCCTTTTAGGTAATATTTTATGATAATAACTACCCCTAGTAAATGATCCCAAGTTGCTTCTGAACCATACCCTAGGAGGTCACCCTTTCAAAAAGGAAAAGAAGACTCTTTGGGAGGACTTTAGCAGAGGCTTTTGCTGGATTATTTGGCAAGAAACAGAAGATTTTTTCAAGATAAGGAATGCTCTTTTTGTAGAATTTTTTTATGCTATAGTCAATATGGTTGTGTCTTGGTGTAAATGCTCTCCCCTTTTCCATGACTATAGCTTTTCTTCCCTCCTTTCTAATTGGAGATGCCTTTTGTAAATCTTTCCTTTGGGGATTTCCCCCTTTTTTGTATTTTGTAATGTCATACCATCAATGAAATTGTTTCTTGTAAATATATATATATACACATATATATATATAATAATGATAACTACCCCTCCAGGCAACCTATTGGTTTTGTAAAATGAAAATCGTCCTATAGCTAGTATCTTTTCTTTTTTGAGCATTTTTTTGGAGATCTAATCTTTTATTTTTGCAACCTCCCTTTAAATAGTACTTTGTATGAACTGTTTTGACAGCTTGCAATATGATGGAAAGATGAGAAATAATCTTATTGCAGGTCTAAACTATATTGCCATCTTTCCTCTGAGAAGTATGAAATGCTTGATAAACTTGTATTTTGAAGGAACTCCGGAAGAAAACAGTTCAATGTATTTCTATGTTAACCCTTATATCCATGACAACTTGCAAGATTAATCCAAATTACATTACTATTATTTGAGCCAGTTCATTTACAGAGAAATAAACCTGGTTAAATTGGTATCTATCAAATTGTTTATTCATGAGACTTTTTTCTTGATCCTGATTGTCAGAATGTTAAGAAGCATTTTTTTAAAATATGGATGAATCGTCATTTTCTTCTATGTACTGTTTAATTTTTAAGTTGAAGTGTCCAAATATAGTTACTACCTGGTACTTTGTTTTTGATTTGTGTACAATTAGGGAGCAGCTGGGGGAGCAGAAGCAAGGCCTTTTGTTACATACCACAATTCCCTGGGGAGAGATCTGTATTTAAGAATAGCTACTGAGCTCCACTTGAAGAGAATGCTAGTGAGTAATTGA

mRNA sequence

ATGGAAGCCTTGAAGCTCTACGGGACTATTTGCTCCCAGCCATTCTTCAAGCATCTCATTCGCTTAGCTTCTTCCTCTTCTACAATCACCGCCCTTGCTCCCAAATCTTCTCGTTCCACCATTTTCCTCCGCCGCTGTTCTTCCACTGCAACCTCCACTGCTGGAGACGGAAAGGTCAAGCTCTCCGGGCGCAGCCGCCGTGCTTCCTCCTCGACTACATCGTCCCCTTCTTCCACGTCTGATAGGGACGCGGTTCGTGCAATTCGCTTAAAGAAGGTTGAAGAATTGAGGAGCAAGGGCTTGGAACCTTATGCGTATACGTGGGACAGGACTCATACTGCCAGTCAGCTGCAAGAGTTGTATAAGCATTTAGGCAATGGTGAAGAGTCTAATAGCGACACTGATCGTGTGTCAATAGCTGGAAGAATTGTGGCTCGCCGAGCGTTTGGAAAGCTTGCATTTCTGACATTAAGAGATGGTTCTGGTACAATTCAGCTTTACTGTGAGAAGGAAAGGCTCATAGATGATCAGTTCGATCAGCTAAAGAATCTTGTTGATATTGGTGATATTCTTGGTGTGCGAGGTTCAATTAAGCGAACTGAGAAAGGTGAACTTTCTGTTTGTTTGAATTCTTTTGAAATCCTGACAAAGTCCCTACTTCCACTTCCTGACAAATATCATGGTTTAACTGATGTTGATAAGCGCTACCGCCAAAGGTATGTAGATATGATTGCAAATCCTGAAGTAGCTGATACATTCCGGAAAAGAGCAAAGATAATATCAGAGATTCGCAAGACCGTGGAATCATTAGGATTTGTTGAAGTTGAGACTCCAGTCCTACAGGGAGCAGCTGGGGGAGCAGAAGCAAGGCCTTTTGTTACATACCACAATTCCCTGGGGAGAGATCTGTATTTAAGAATAGCTACTGAGCTCCACTTGAAGAGAATGCTAGTGAGTAATTGA

Coding sequence (CDS)

ATGGAAGCCTTGAAGCTCTACGGGACTATTTGCTCCCAGCCATTCTTCAAGCATCTCATTCGCTTAGCTTCTTCCTCTTCTACAATCACCGCCCTTGCTCCCAAATCTTCTCGTTCCACCATTTTCCTCCGCCGCTGTTCTTCCACTGCAACCTCCACTGCTGGAGACGGAAAGGTCAAGCTCTCCGGGCGCAGCCGCCGTGCTTCCTCCTCGACTACATCGTCCCCTTCTTCCACGTCTGATAGGGACGCGGTTCGTGCAATTCGCTTAAAGAAGGTTGAAGAATTGAGGAGCAAGGGCTTGGAACCTTATGCGTATACGTGGGACAGGACTCATACTGCCAGTCAGCTGCAAGAGTTGTATAAGCATTTAGGCAATGGTGAAGAGTCTAATAGCGACACTGATCGTGTGTCAATAGCTGGAAGAATTGTGGCTCGCCGAGCGTTTGGAAAGCTTGCATTTCTGACATTAAGAGATGGTTCTGGTACAATTCAGCTTTACTGTGAGAAGGAAAGGCTCATAGATGATCAGTTCGATCAGCTAAAGAATCTTGTTGATATTGGTGATATTCTTGGTGTGCGAGGTTCAATTAAGCGAACTGAGAAAGGTGAACTTTCTGTTTGTTTGAATTCTTTTGAAATCCTGACAAAGTCCCTACTTCCACTTCCTGACAAATATCATGGTTTAACTGATGTTGATAAGCGCTACCGCCAAAGGTATGTAGATATGATTGCAAATCCTGAAGTAGCTGATACATTCCGGAAAAGAGCAAAGATAATATCAGAGATTCGCAAGACCGTGGAATCATTAGGATTTGTTGAAGTTGAGACTCCAGTCCTACAGGGAGCAGCTGGGGGAGCAGAAGCAAGGCCTTTTGTTACATACCACAATTCCCTGGGGAGAGATCTGTATTTAAGAATAGCTACTGAGCTCCACTTGAAGAGAATGCTAGTGAGTAATTGA

Protein sequence

MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVKLSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQLKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYVDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLGRDLYLRIATELHLKRMLVSN

Homology

[Close]

BLAST of Spg001335 vs. NCBI nr
Match: XP_023534158.1 (lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 301/318 (94.65%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALKLYG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FLRRCSS ATSTAGDGKVK
Sbjct: 1   MEALKLYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLRRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSSPSSTSDR+AVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSPSSTSDREAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESNSDT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNSDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTD+DKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDIDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. NCBI nr
Match: KAG6605831.1 (Lysine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 582.4 bits (1500), Expect = 2.4e-162
Identity = 300/318 (94.34%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALKLYG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FLRRCSS ATSTAGDGKVK
Sbjct: 1   MEALKLYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLRRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSSP+STSDR+AVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSPASTSDREAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESNSDT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNSDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTD+DKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDIDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. NCBI nr
Match: KAG7035789.1 (Lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.4 bits (1500), Expect = 2.4e-162
Identity = 300/318 (94.34%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALKLYG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FLRRCSS ATSTAGDGKVK
Sbjct: 1   MEALKLYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLRRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSSP+STSDR+AVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSPASTSDREAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESNSDT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNSDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTD+DKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDIDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. NCBI nr
Match: XP_022958622.1 (lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 580.1 bits (1494), Expect = 1.2e-161
Identity = 298/318 (93.71%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALKLYG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FLRRCSS ATSTAGDGKVK
Sbjct: 1   MEALKLYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLRRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSSP+STSDR+AVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSPASTSDREAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESN+DT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNNDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTD+DKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDIDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIR+TVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRRTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. NCBI nr
Match: XP_022995985.1 (lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 575.9 bits (1483), Expect = 2.2e-160
Identity = 298/318 (93.71%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALK YG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FL RCSS ATSTAGDGKVK
Sbjct: 1   MEALKFYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLSRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSS SSTSDR+AVRAIRLKKVEELRSKGLEPYAYTWD+THTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSSSSTSDREAVRAIRLKKVEELRSKGLEPYAYTWDKTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESNSDT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNSDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTDVDKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. ExPASy Swiss-Prot
Match: Q9LJE2 (Lysine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA5 PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.1e-121
Identity = 230/323 (71.21%), Postives = 275/323 (85.14%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTI-TALAPKSSRSTIFLRRCSSTATSTAGDGKV 60
           MEALK++ ++ + P  K L+RL+SSS+ + T +  + S       RC+S A+S++     
Sbjct: 1   MEALKVW-SLTATP-LKQLLRLSSSSTRLATTIYGRRSYHLSPALRCASAASSSSSSATT 60

Query: 61  ----KLSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTAS 120
               K SGR+RR++SS+    +STSDR+A+R+IRLKKVEELR +GLEPYAY W+++H+A+
Sbjct: 61  AETSKPSGRNRRSASSS----NSTSDREAIRSIRLKKVEELRGQGLEPYAYKWEKSHSAN 120

Query: 121 QLQELYKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLID 180
           QLQE+YKHL NGEES+++ D VSIAGR+VARRAFGKLAFLTLRD SGTIQLYCEKERL D
Sbjct: 121 QLQEIYKHLANGEESDNEIDCVSIAGRVVARRAFGKLAFLTLRDDSGTIQLYCEKERLSD 180

Query: 181 DQFDQLKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKR 240
           DQF+QLK  +DIGDILG  GS+KRTEKGELS+C+NSF ILTKSLLPLPDKYHGLTD+DKR
Sbjct: 181 DQFEQLKQFIDIGDILGASGSMKRTEKGELSICVNSFSILTKSLLPLPDKYHGLTDIDKR 240

Query: 241 YRQRYVDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTY 300
           YRQRYVDMIANPEVAD FR+RAKI+SEIRKTVES G++EVETPVLQGAAGGAEARPFVT+
Sbjct: 241 YRQRYVDMIANPEVADVFRRRAKIVSEIRKTVESFGYLEVETPVLQGAAGGAEARPFVTF 300

Query: 301 HNSLGRDLYLRIATELHLKRMLV 319
           HNSLGRDLYLRIATELHLKRMLV
Sbjct: 301 HNSLGRDLYLRIATELHLKRMLV 317

[Close]

BLAST of Spg001335 vs. ExPASy Swiss-Prot
Match: P73443 (Lysine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lysS PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 153/247 (61.94%), Postives = 194/247 (78.54%), Query Frame = 0


Query: 78  STSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLGNGEESNSDTDRV 137
           S S  + +RA RL+K E+LR  GL PYAYTW+ TH A  LQE Y+ L NGEE +    +V
Sbjct: 6   SHSSLEEIRATRLEKAEQLRQLGLNPYAYTWEITHQAQDLQETYRDLSNGEEVDL---KV 65

Query: 138 SIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERL------IDDQFDQLKNLVDIGDIL 197
           +IAGRI+ARR  GKLAF TL+D SGTIQLY EK+RL      +++ F+ LK + D+GDIL
Sbjct: 66  AIAGRILARRVMGKLAFFTLQDESGTIQLYLEKQRLTEHMPELENAFNLLKKITDVGDIL 125

Query: 198 GVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYVDMIANPEVAD 257
           GV G++KRTEKGELSV + ++ +LTKSLLPLPDK+HGLTD +KRYRQRYVD+I NP V  
Sbjct: 126 GVTGTLKRTEKGELSVYVQTYAVLTKSLLPLPDKWHGLTDTEKRYRQRYVDLIVNPTVRQ 185

Query: 258 TFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLGRDLYLRIATEL 317
           TFR+RA+I + IR+ ++  GF+E+ETPVLQG +GGAEARPF+T+HN+LG  LYLRIATEL
Sbjct: 186 TFRRRAQITAAIRRYLDKQGFIEIETPVLQGESGGAEARPFITHHNTLGMPLYLRIATEL 245

Query: 318 HLKRMLV 319
           HLKR++V
Sbjct: 246 HLKRLVV 249

[Close]

BLAST of Spg001335 vs. ExPASy Swiss-Prot
Match: Q31KH4 (Lysine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=lysS PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 154/241 (63.90%), Postives = 189/241 (78.42%), Query Frame = 0


Query: 85  VRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLGNGEESNSDTDRVSIAGRIV 144
           +RA RL+K  +LR  G EPYAY WD +H+A+QLQ LY  L  GEE   +   V+IAGRI+
Sbjct: 4   LRATRLEKALQLRELGFEPYAYRWDCSHSAAQLQALYADLPAGEEVAVE---VAIAGRIM 63

Query: 145 ARRAFGKLAFLTLRDGSGTIQLYCEKERLID-------DQFDQLKNLVDIGDILGVRGSI 204
           ARR FGKLAF TL+D SG IQLY EK+RL +       + F  LK L D+GDILGV+GS+
Sbjct: 64  ARRVFGKLAFFTLQDESGQIQLYLEKQRLSESMAAIDAEAFSHLKALTDVGDILGVKGSV 123

Query: 205 KRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYVDMIANPEVADTFRKRA 264
           +RTEKGELS+ ++ + ILTKSL PLPDK+HGLTDV+KRYRQRYVD+I NPEV +TFR+RA
Sbjct: 124 RRTEKGELSIAVDQYAILTKSLQPLPDKWHGLTDVEKRYRQRYVDLIVNPEVRETFRRRA 183

Query: 265 KIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLGRDLYLRIATELHLKRML 319
           KI + IR+ +E  GF+E+ETPVLQ  AGGAEARPF+TYHN+L  DLYLRIATELHLKR++
Sbjct: 184 KITAAIRRHLEDQGFIEIETPVLQAEAGGAEARPFITYHNTLELDLYLRIATELHLKRLI 241

[Close]

BLAST of Spg001335 vs. ExPASy Swiss-Prot
Match: Q5N1D9 (Lysine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=lysS PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 154/241 (63.90%), Postives = 189/241 (78.42%), Query Frame = 0


Query: 85  VRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLGNGEESNSDTDRVSIAGRIV 144
           +RA RL+K  +LR  G EPYAY WD +H+A+QLQ LY  L  GEE   +   V+IAGRI+
Sbjct: 4   LRATRLEKALQLRELGFEPYAYRWDCSHSAAQLQALYADLPAGEEVAVE---VAIAGRIM 63

Query: 145 ARRAFGKLAFLTLRDGSGTIQLYCEKERLID-------DQFDQLKNLVDIGDILGVRGSI 204
           ARR FGKLAF TL+D SG IQLY EK+RL +       + F  LK L D+GDILGV+GS+
Sbjct: 64  ARRVFGKLAFFTLQDESGQIQLYLEKQRLSESMAAIDAEAFSHLKALTDVGDILGVKGSV 123

Query: 205 KRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYVDMIANPEVADTFRKRA 264
           +RTEKGELS+ ++ + ILTKSL PLPDK+HGLTDV+KRYRQRYVD+I NPEV +TFR+RA
Sbjct: 124 RRTEKGELSIAVDQYAILTKSLQPLPDKWHGLTDVEKRYRQRYVDLIVNPEVRETFRRRA 183

Query: 265 KIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLGRDLYLRIATELHLKRML 319
           KI + IR+ +E  GF+E+ETPVLQ  AGGAEARPF+TYHN+L  DLYLRIATELHLKR++
Sbjct: 184 KITAAIRRHLEDQGFIEIETPVLQAEAGGAEARPFITYHNTLELDLYLRIATELHLKRLI 241

[Close]

BLAST of Spg001335 vs. ExPASy Swiss-Prot
Match: Q8DMA9 (Lysine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=lysS PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.8e-81
Identity = 150/238 (63.03%), Postives = 188/238 (78.99%), Query Frame = 0


Query: 85  VRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLGNGEESNSDTDRVSIAGRIV 144
           +RA R++K + L+++G+ PYAY W+RTHTA+ LQ+ Y HL  GE      DRVS+AGRI+
Sbjct: 11  IRATRIEKAKTLQAQGMSPYAYRWERTHTAALLQDKYAHLAAGEAVG---DRVSVAGRIM 70

Query: 145 ARRAFGKLAFLTLRDGSGTIQLYCEKERLI----DDQFDQLKNLVDIGDILGVRGSIKRT 204
           ARR FGKLAF TL+D SGTIQLY +K+ +     +  F  LK+L D+GDILG  G++KRT
Sbjct: 71  ARRIFGKLAFFTLQDDSGTIQLYLDKQTISQTMGEAAFADLKHLTDVGDILGAVGTLKRT 130

Query: 205 EKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRYVDMIANPEVADTFRKRAKII 264
           EKGELSV + S+ +LTKSLLPLPDK+HGLTDV+KRYRQRYVD+I NP+V DTFRKRA I 
Sbjct: 131 EKGELSVVVESYTMLTKSLLPLPDKWHGLTDVEKRYRQRYVDLIVNPQVRDTFRKRALIT 190

Query: 265 SEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLGRDLYLRIATELHLKRMLV 319
           + IR+ +   GF+E+ETPVLQ  AGGAEARPF+TYHN+L   LYLRIATELHLKR++V
Sbjct: 191 AAIRRYLNEQGFIEIETPVLQVEAGGAEARPFITYHNTLEMQLYLRIATELHLKRLIV 245

[Close]

BLAST of Spg001335 vs. ExPASy TrEMBL
Match: A0A6J1H2C3 (Lysine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111459793 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.7e-162
Identity = 298/318 (93.71%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALKLYG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FLRRCSS ATSTAGDGKVK
Sbjct: 1   MEALKLYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLRRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSSP+STSDR+AVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSPASTSDREAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESN+DT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNNDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTD+DKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDIDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIR+TVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRRTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. ExPASy TrEMBL
Match: A0A6J1K5F6 (Lysine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111491333 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 298/318 (93.71%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALK YG +CSQP FKHLIRLASSSSTI+ALAPKSSRS +FL RCSS ATSTAGDGKVK
Sbjct: 1   MEALKFYGAVCSQPLFKHLIRLASSSSTISALAPKSSRSALFLSRCSSAATSTAGDGKVK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGRSRR+SSSTTSS SSTSDR+AVRAIRLKKVEELRSKGLEPYAYTWD+THTASQLQEL
Sbjct: 61  LSGRSRRSSSSTTSSSSSTSDREAVRAIRLKKVEELRSKGLEPYAYTWDKTHTASQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESNSDT  VSIAGRIVARRAFGKLAFLTLRD SGTIQLYCEKERLI+DQFDQ
Sbjct: 121 YKHLGNGEESNSDTSSVSIAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLINDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILG RGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTDVDKRYRQRY
Sbjct: 181 LKNLVDIGDILGARGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNS+G
Sbjct: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSIG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. ExPASy TrEMBL
Match: A0A0A0L1N3 (Lysine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G268050 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 292/318 (91.82%), Postives = 306/318 (96.23%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEA   Y TIC+QPFFKHL RLASS+STITAL PKSSRST FLRRCSS ATSTAGDGK+K
Sbjct: 1   MEASNPYATICAQPFFKHLSRLASSTSTITALVPKSSRSTRFLRRCSSAATSTAGDGKLK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGR+RR+SSSTTS PSSTSDR+AVRAIRLKKVEELRSKG EPYAYTWDRTHTA+QLQEL
Sbjct: 61  LSGRTRRSSSSTTSPPSSTSDREAVRAIRLKKVEELRSKGFEPYAYTWDRTHTANQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESN+DTD VS+AGRIVARRAFGKLAFLTLRD SGTIQLYCEKERL++DQFDQ
Sbjct: 121 YKHLGNGEESNADTDCVSVAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLVNDQFDQ 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILGVRGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTDVDKRYRQRY
Sbjct: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           +DMIANPEVADTFRKRAKIISE+RKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG
Sbjct: 241 IDMIANPEVADTFRKRAKIISEVRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. ExPASy TrEMBL
Match: A0A5A7TFD0 (Lysine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00900 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.0e-158
Identity = 292/318 (91.82%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALK YGTICSQPFFKH+IRL SS+STITAL PKSSRS  FLR CSS A STAGDGK+K
Sbjct: 1   MEALKPYGTICSQPFFKHVIRLPSSTSTITALVPKSSRSAHFLRCCSSAAISTAGDGKLK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGR+RR+SSSTTS PSSTSDR+AVRAIRLKKVEELRSKG EPYAYTWDRTHTA+QLQEL
Sbjct: 61  LSGRTRRSSSSTTSPPSSTSDREAVRAIRLKKVEELRSKGFEPYAYTWDRTHTANQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESN+DTD VS+AGRIVARRAFGKLAFLTLRD SGTIQLYCEKERL++DQFD 
Sbjct: 121 YKHLGNGEESNADTDCVSVAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLVNDQFDH 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILGVRGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTDVDKRYRQRY
Sbjct: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISE+RKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG
Sbjct: 241 VDMIANPEVADTFRKRAKIISEVRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. ExPASy TrEMBL
Match: A0A1S3BP13 (Lysyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103491634 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.0e-158
Identity = 292/318 (91.82%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTITALAPKSSRSTIFLRRCSSTATSTAGDGKVK 60
           MEALK YGTICSQPFFKH+IRL SS+STITAL PKSSRS  FLR CSS A STAGDGK+K
Sbjct: 1   MEALKPYGTICSQPFFKHVIRLPSSTSTITALVPKSSRSAHFLRCCSSAAISTAGDGKLK 60

Query: 61  LSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQEL 120
           LSGR+RR+SSSTTS PSSTSDR+AVRAIRLKKVEELRSKG EPYAYTWDRTHTA+QLQEL
Sbjct: 61  LSGRTRRSSSSTTSPPSSTSDREAVRAIRLKKVEELRSKGFEPYAYTWDRTHTANQLQEL 120

Query: 121 YKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLIDDQFDQ 180
           YKHLGNGEESN+DTD VS+AGRIVARRAFGKLAFLTLRD SGTIQLYCEKERL++DQFD 
Sbjct: 121 YKHLGNGEESNADTDCVSVAGRIVARRAFGKLAFLTLRDDSGTIQLYCEKERLVNDQFDH 180

Query: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240
           LKNLVDIGDILGVRGSIKRTEKGELSVC+NSFEILTKSLLPLPDKYHGLTDVDKRYRQRY
Sbjct: 181 LKNLVDIGDILGVRGSIKRTEKGELSVCVNSFEILTKSLLPLPDKYHGLTDVDKRYRQRY 240

Query: 241 VDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300
           VDMIANPEVADTFRKRAKIISE+RKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG
Sbjct: 241 VDMIANPEVADTFRKRAKIISEVRKTVESLGFVEVETPVLQGAAGGAEARPFVTYHNSLG 300

Query: 301 RDLYLRIATELHLKRMLV 319
           RDLYLRIATELHLKRMLV
Sbjct: 301 RDLYLRIATELHLKRMLV 318

[Close]

BLAST of Spg001335 vs. TAIR 10
Match: AT3G13490.1 (Lysyl-tRNA synthetase, class II )

HSP 1 Score: 435.3 bits (1118), Expect = 4.4e-122
Identity = 230/323 (71.21%), Postives = 275/323 (85.14%), Query Frame = 0


Query: 1   MEALKLYGTICSQPFFKHLIRLASSSSTI-TALAPKSSRSTIFLRRCSSTATSTAGDGKV 60
           MEALK++ ++ + P  K L+RL+SSS+ + T +  + S       RC+S A+S++     
Sbjct: 1   MEALKVW-SLTATP-LKQLLRLSSSSTRLATTIYGRRSYHLSPALRCASAASSSSSSATT 60

Query: 61  ----KLSGRSRRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTAS 120
               K SGR+RR++SS+    +STSDR+A+R+IRLKKVEELR +GLEPYAY W+++H+A+
Sbjct: 61  AETSKPSGRNRRSASSS----NSTSDREAIRSIRLKKVEELRGQGLEPYAYKWEKSHSAN 120

Query: 121 QLQELYKHLGNGEESNSDTDRVSIAGRIVARRAFGKLAFLTLRDGSGTIQLYCEKERLID 180
           QLQE+YKHL NGEES+++ D VSIAGR+VARRAFGKLAFLTLRD SGTIQLYCEKERL D
Sbjct: 121 QLQEIYKHLANGEESDNEIDCVSIAGRVVARRAFGKLAFLTLRDDSGTIQLYCEKERLSD 180

Query: 181 DQFDQLKNLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDKYHGLTDVDKR 240
           DQF+QLK  +DIGDILG  GS+KRTEKGELS+C+NSF ILTKSLLPLPDKYHGLTD+DKR
Sbjct: 181 DQFEQLKQFIDIGDILGASGSMKRTEKGELSICVNSFSILTKSLLPLPDKYHGLTDIDKR 240

Query: 241 YRQRYVDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQGAAGGAEARPFVTY 300
           YRQRYVDMIANPEVAD FR+RAKI+SEIRKTVES G++EVETPVLQGAAGGAEARPFVT+
Sbjct: 241 YRQRYVDMIANPEVADVFRRRAKIVSEIRKTVESFGYLEVETPVLQGAAGGAEARPFVTF 300

Query: 301 HNSLGRDLYLRIATELHLKRMLV 319
           HNSLGRDLYLRIATELHLKRMLV
Sbjct: 301 HNSLGRDLYLRIATELHLKRMLV 317

[Close]

BLAST of Spg001335 vs. TAIR 10
Match: AT3G11710.1 (lysyl-tRNA synthetase 1 )

HSP 1 Score: 142.9 bits (359), Expect = 4.5e-34
Identity = 97/277 (35.02%), Postives = 150/277 (54.15%), Query Frame = 0


Query: 66  RRASSSTTSSPSSTSDRDAVRAIRLKKVEELRSKGLEPYAYTWDRTHTASQLQELYKHLG 125
           + +S    ++     D       RLK +   ++KG  PY + +  + +  +  E Y  L 
Sbjct: 71  KASSQKAVAADDEEMDATQYYENRLKYLAAEKAKGENPYPHKFAVSMSIPKYIETYGSLN 130

Query: 126 NGEESNSDTDRVSIAGRIVARR-AFGKLAFLTLRDGSGTIQLYCE--KERLIDDQFDQLK 185
           NG+   +  +  S+AGRI+++R +  KL F  L      +Q+  +  K  L + +F +L 
Sbjct: 131 NGDHVENAEE--SLAGRIMSKRSSSSKLFFYDLHGDDFKVQVMADASKSGLDEAEFLKLH 190

Query: 186 NLVDIGDILGVRGSIKRTEKGELSVCLNSFEILTKSLLPLPDK----------------- 245
           +    GDI+GV G   +T++GELS+   SF +L+  L  +P K                 
Sbjct: 191 SNAKRGDIVGVIGFPGKTKRGELSIFPRSFILLSHCLHMMPRKADNVNAKKPEIWVPGQT 250

Query: 246 ----YHGLTDVDKRYRQRYVDMIANPEVADTFRKRAKIISEIRKTVESLGFVEVETPVLQ 305
                + L D + RYRQR++DMI N EV   FR RAKIIS +R+ +++  F+EVETP++ 
Sbjct: 251 RNPEAYVLKDQESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFLDNKNFLEVETPMMN 310

Query: 306 GAAGGAEARPFVTYHNSLGRDLYLRIATELHLKRMLV 319
             AGGA ARPFVT+HN L   LY+RIA EL+LK+++V
Sbjct: 311 MIAGGAAARPFVTHHNDLDMRLYMRIAPELYLKQLIV 345

The following BLAST results are available for this feature:

BLAST of Spg001335 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534158.1	1.1e-162	94.65	lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]	[more]
KAG6605831.1	2.4e-162	94.34	Lysine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperm...	[more]
KAG7035789.1	2.4e-162	94.34	Lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. ...	[more]
XP_022958622.1	1.2e-161	93.71	lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]
XP_022995985.1	2.2e-160	93.71	lysine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima]	[more]

BLAST of Spg001335 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJE2	6.1e-121	71.21	Lysine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
P73443	1.1e-82	61.94	Lysine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 G...	[more]
Q31KH4	1.3e-81	63.90	Lysine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=...	[more]
Q5N1D9	1.3e-81	63.90	Lysine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 14...	[more]
Q8DMA9	4.8e-81	63.03	Lysine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=...	[more]

BLAST of Spg001335 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H2C3	5.7e-162	93.71	Lysine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111459793 PE=3 SV=1	[more]
A0A6J1K5F6	1.1e-160	93.71	Lysine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111491333 PE=3 SV=1	[more]
A0A0A0L1N3	1.3e-158	91.82	Lysine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G268050 PE=3 SV=1	[more]
A0A5A7TFD0	5.0e-158	91.82	Lysine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G...	[more]
A0A1S3BP13	5.0e-158	91.82	Lysyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103491634 PE=3 SV=1	[more]

BLAST of Spg001335 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13490.1	4.4e-122	71.21	Lysyl-tRNA synthetase, class II	[more]
AT3G11710.1	4.5e-34	35.02	lysyl-tRNA synthetase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018149	Lysyl-tRNA synthetase, class II, C-terminal	PRINTS	PR00982	TRNASYNTHLYS	coord: 313..320 score: 23.54 coord: 284..300 score: 63.18 coord: 268..278 score: 63.97
None	No IPR available	GENE3D	2.40.50.140		coord: 83..224 e-value: 6.1E-46 score: 157.8
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 225..320 e-value: 6.5E-33 score: 116.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..82
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..84
None	No IPR available	PANTHER	PTHR42918	LYSYL-TRNA SYNTHETASE	coord: 66..319
None	No IPR available	PANTHER	PTHR42918:SF9	LYSINE--TRNA LIGASE	coord: 66..319
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 231..319
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 137..215 e-value: 1.5E-18 score: 66.6
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 232..319 e-value: 3.9E-17 score: 62.3
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 253..320 score: 10.83485
IPR044136	Lysine-tRNA ligase, class II, N-terminal	CDD	cd04322	LysRS_N	coord: 136..243 e-value: 5.20744E-54 score: 170.351
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 82..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg001335.1	Spg001335.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006430	lysyl-tRNA aminoacylation
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004824	lysine-tRNA ligase activity
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding