Spg001307 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg001307
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Poly(A)-specific ribonuclease
Location	scaffold8: 39048485 .. 39049568 (-)
RNA-Seq Expression	Spg001307
Synteny	Spg001307

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAAAATCTCATTGCGATTTCAGAGAGTGTTTTATGGGTTTTGGATCGATGAAGAAGAACAGGATGAGCCGGGAGAATCGATGGGGTCCGAGAAGACGAAAGCCGATGATTCGAGAAGTCTGGGCTTGGAATTTCGAGTCCGAAATCTCCCGCTTCGATCGCTGCCTTGAATCGTACACGATTCTGTCGATCGACACGGAATTTCCTGGGTTTATTCAGACTACTCCTTGGGGCTCCATCGACGAACAAATTTATCAGAACATGCGGGTCAATGTCAATCAGACGAAATTGATCCAACTGGGTCTGACCGCCTCCGATGAATGCGGAAAGATCGGCGGCGCCTGGGAGTTCAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCCTCCATTTCATTTCTGAAACAGAACGGGCTGGATTTCGACAGACTCAAATCAGACGGGATTCCACTGGTTACATTCACGGCAAATTTCGTACAAATCTTAAGAAAGAACCAAATCTTCCGATGGGTCACATTCCACGGCCTGTACGATATCGGGTAAGTAGAAATTAAATTTCACCGCCGATGATCAAACTCTTCGAATTCTCGAATTCCATTTGGTTTCGACAATACAGAGATTCACTGAATTAATTGTTGTTTCCTCAACAGATATCTCGTGAAAGCGATGGGGGTAGTCCAGGAGTTGCCGGAATCAATGGAGGAATTCGGGAACTTAGTGGTGAATGCAGTGGGCAGCGTGCGCGATCTGAAGCACATCGCTCGGTTCTGCGAAGGGCTGGAAGATGGGAAACTCGGCCTGGAAAGACTGGGCGAACTTTTGGAGCAAAAGCGGTTAGGATCGAAGCACCACGCCGGGTCGGACAGTCTGCTGACGGCTGCGCTCCACACGAAGATAATGGAACGGTTCGAACCGGGTTCGGAAATCTGCGACGGGTTTCTGTATGGGCTGTCGAATAAACTCCAAAACATGGAGATGATGAAGATGAAGGCAGTTCAGACGATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTATTTCCCTTGTTACTATTACTGAAAATTGAAC

mRNA sequence

ATGGGTTTTGGATCGATGAAGAAGAACAGGATGAGCCGGGAGAATCGATGGGGTCCGAGAAGACGAAAGCCGATGATTCGAGAAGTCTGGGCTTGGAATTTCGAGTCCGAAATCTCCCGCTTCGATCGCTGCCTTGAATCGTACACGATTCTGTCGATCGACACGGAATTTCCTGGGTTTATTCAGACTACTCCTTGGGGCTCCATCGACGAACAAATTTATCAGAACATGCGGGTCAATGTCAATCAGACGAAATTGATCCAACTGGGTCTGACCGCCTCCGATGAATGCGGAAAGATCGGCGGCGCCTGGGAGTTCAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCCTCCATTTCATTTCTGAAACAGAACGGGCTGGATTTCGACAGACTCAAATCAGACGGGATTCCACTGGTTACATTCACGGCAAATTTCGTACAAATCTTAAGAAAGAACCAAATCTTCCGATGGGTCACATTCCACGGCCTGTACGATATCGGATATCTCGTGAAAGCGATGGGGGTAGTCCAGGAGTTGCCGGAATCAATGGAGGAATTCGGGAACTTAGTGGTGAATGCAGTGGGCAGCGTGCGCGATCTGAAGCACATCGCTCGGTTCTGCGAAGGGCTGGAAGATGGGAAACTCGGCCTGGAAAGACTGGGCGAACTTTTGGAGCAAAAGCGGTTAGGATCGAAGCACCACGCCGGGTCGGACAGTCTGCTGACGGCTGCGCTCCACACGAAGATAATGGAACGGTTCGAACCGGGTTCGGAAATCTGCGACGGGTTTCTGTATGGGCTGTCGAATAAACTCCAAAACATGGAGATGATGAAGATGAAGGCAGTTCAGACGATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTATTTCCCTTGTTACTATTACTGA

Coding sequence (CDS)

ATGGGTTTTGGATCGATGAAGAAGAACAGGATGAGCCGGGAGAATCGATGGGGTCCGAGAAGACGAAAGCCGATGATTCGAGAAGTCTGGGCTTGGAATTTCGAGTCCGAAATCTCCCGCTTCGATCGCTGCCTTGAATCGTACACGATTCTGTCGATCGACACGGAATTTCCTGGGTTTATTCAGACTACTCCTTGGGGCTCCATCGACGAACAAATTTATCAGAACATGCGGGTCAATGTCAATCAGACGAAATTGATCCAACTGGGTCTGACCGCCTCCGATGAATGCGGAAAGATCGGCGGCGCCTGGGAGTTCAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCCTCCATTTCATTTCTGAAACAGAACGGGCTGGATTTCGACAGACTCAAATCAGACGGGATTCCACTGGTTACATTCACGGCAAATTTCGTACAAATCTTAAGAAAGAACCAAATCTTCCGATGGGTCACATTCCACGGCCTGTACGATATCGGATATCTCGTGAAAGCGATGGGGGTAGTCCAGGAGTTGCCGGAATCAATGGAGGAATTCGGGAACTTAGTGGTGAATGCAGTGGGCAGCGTGCGCGATCTGAAGCACATCGCTCGGTTCTGCGAAGGGCTGGAAGATGGGAAACTCGGCCTGGAAAGACTGGGCGAACTTTTGGAGCAAAAGCGGTTAGGATCGAAGCACCACGCCGGGTCGGACAGTCTGCTGACGGCTGCGCTCCACACGAAGATAATGGAACGGTTCGAACCGGGTTCGGAAATCTGCGACGGGTTTCTGTATGGGCTGTCGAATAAACTCCAAAACATGGAGATGATGAAGATGAAGGCAGTTCAGACGATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTATTTCCCTTGTTACTATTACTGA

Protein sequence

MGFGSMKKNRMSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFEPGSEICDGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSIPVYYFPCYYY

Homology

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BLAST of Spg001307 vs. NCBI nr
Match: XP_038895744.1 (putative CCR4-associated factor 1 homolog 8 [Benincasa hispida])

HSP 1 Score: 406.8 bits (1044), Expect = 1.7e-109
Identity = 195/294 (66.33%), Postives = 243/294 (82.65%), Query Frame = 0


Query: 20  RRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRV 79
           R RK +I +VWA NF++EIS+ + CLE +TI+SIDTEFPGFI+ +PWGSIDE +Y++ R 
Sbjct: 5   RIRKSLIHKVWASNFDAEISKLEECLEFHTIVSIDTEFPGFIRNSPWGSIDETLYEDFRF 64

Query: 80  NVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSD 139
           NVNQTKLIQLGLTA D+ GKIGG+WEFNF DFD E DA +PSS SFL+QNGLDF++LK  
Sbjct: 65  NVNQTKLIQLGLTACDDSGKIGGSWEFNFSDFDPEIDAQSPSSASFLEQNGLDFNKLKKY 124

Query: 140 GIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAV 199
           GIP+  FTA FV I+RK+ +FRWVTFHGLYDIGYL+KAMG+ + LPE+MEEF  +VV  +
Sbjct: 125 GIPINLFTAKFVHIIRKHNVFRWVTFHGLYDIGYLIKAMGITEVLPETMEEFATVVVRRL 184

Query: 200 GSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFE 259
           G VRDLKH+ARFCEGLEDGKLGLERLG+LL+QKR G KH+AGSDSLLTA+ H ++++RF+
Sbjct: 185 GIVRDLKHMARFCEGLEDGKLGLERLGKLLDQKRYGLKHNAGSDSLLTASAHFEMIQRFD 244

Query: 260 PGSEICDGFLYGLSNKLQNMEM-MKMKAVQTITFGRIPVSIPVY-YFPCY--YY 310
             +EICDG+LYG SNKLQ+M++ M M   + ITFG+IP +IP+Y Y P Y  YY
Sbjct: 245 MDAEICDGYLYGFSNKLQSMDINMNMFHRKIITFGQIPYAIPLYCYVPSYITYY 298

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BLAST of Spg001307 vs. NCBI nr
Match: KAG6604931.1 (putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027010.1 putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.4 bits (986), Expect = 9.1e-103
Identity = 187/289 (64.71%), Postives = 225/289 (77.85%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPG I++TPWGSID
Sbjct: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           E++Y+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSISFL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LDF +LK  GIPL  FT NF +I+R++++ +WVTFHGLYDIGYLVKAMGV +ELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAAL 250
           F  +V   VG VRDLK +AR+CEGLE G++GLERLGELL +KR G KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 251 HTKIMERFEPGSEICDGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSI 300
           H K+   F   S   DG LYGL NKL++     MK  QTI FG+I  S+
Sbjct: 241 HAKMARWFRLESGAYDGHLYGLQNKLEH-----MKVNQTIMFGQISCSL 284

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BLAST of Spg001307 vs. NCBI nr
Match: XP_022146113.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 384.4 bits (986), Expect = 9.1e-103
Identity = 186/301 (61.79%), Postives = 227/301 (75.42%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M R  R   RR    IREVW+ NF+ E++  + CL  Y IL+IDTEFPGFI++TPWG+I+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           E++Y + R NVN+TKLIQLGLTASDE G IG  WEFNF DFD + D HN SS+SFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LDFD+LK  GIP+  FT+    + RK++I RWVTFHGLYDIGYL+KAMGV  E+PESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAAL 250
           F  LV  A+G+V DLKH+A+FCEGLEDGKLGLE+L +L+ QKR G+KHHAGSDSLLTA+L
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 251 HTKIMERFEPGSEICDGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSIPVYYFPCY--Y 310
           + K+  +FE GSE+CDGFLYGL  KL      K+K  Q + FG+IP  +   Y PCY  Y
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLH----AKIKLYQNLMFGQIPYGLRACYPPCYIAY 297

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BLAST of Spg001307 vs. NCBI nr
Match: XP_022947867.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 378.6 bits (971), Expect = 5.0e-101
Identity = 180/269 (66.91%), Postives = 215/269 (79.93%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPG I++TPWGSID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           E++Y+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSI+FL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LDF +LK  GIPL  FT NF +I+R++++ +WVTFHGLYDIGYLVKAMGV +ELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAAL 250
           F  +V   VG VRDLK +AR+CEGLE G++GLERLGELL +KR G KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 251 HTKIMERFEPGSEICDGFLYGLSNKLQNM 280
           H K+   F   SE  DG LYGL NKLQ M
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYM 269

[Close]

BLAST of Spg001307 vs. NCBI nr
Match: KAG6604930.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-100
Identity = 172/262 (65.65%), Postives = 211/262 (80.53%), Query Frame = 0


Query: 18  GPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNM 77
           GPR   P+IREVWA+N + EI R DRCL SY+++SIDTEFPG I+ TPWG+IDE++Y + 
Sbjct: 7   GPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADF 66

Query: 78  RVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLK 137
           R NVNQTK+IQLG+T SDE G IGG WEFNF DFD E DAHNP+SI FLKQNGLDF +LK
Sbjct: 67  RFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLK 126

Query: 138 SDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVN 197
            DGI +  F   F+  LRK+ I+RW+TFHGLYDIGYL+ AMGVV+ LPE++ EF  +V  
Sbjct: 127 KDGIKVRKFAIRFIYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVAR 186

Query: 198 AVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMER 257
            VG+VRDLKH+ARFCEGLE G LGLE+LG+LL+QKR G KHHAGSDSL+TA LH K+++R
Sbjct: 187 RVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQR 246

Query: 258 FEPGSEICDGFLYGLSNKLQNM 280
           ++  +EICDGFLYGLS K + +
Sbjct: 247 YDFHAEICDGFLYGLSKKFEEL 268

[Close]

BLAST of Spg001307 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 108/276 (39.13%), Postives = 155/276 (56.16%), Query Frame = 0


Query: 28  EVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLI 87
           EVW WN E E+     CL+ ++ ++IDTEFPG ++ TP  + +E  Y++M+ NV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 88  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFT 147
           QLG T  D  G I   WE N  DF+      N  SI+FLK NGL+ D++  +GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 148 ANFVQILR-KNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-VRDL 207
            +F QIL+ K+    WV F G YD  YLVK +   + LPE+ EEF   V   +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 208 KHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFEPGSEIC 267
           K IA  C GL   + GL+R+ ++L+ KR+G  HHAGSDS LTA + TK+        + C
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKLTFDLLNSRKQC 242

Query: 268 DGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSIPV 302
              +     +L+  ++M  +         IP+ IP+
Sbjct: 243 VRRVDHQQYQLEQQQLMMTRCY-------IPIPIPI 269

[Close]

BLAST of Spg001307 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.7e-47
Identity = 97/252 (38.49%), Postives = 146/252 (57.94%), Query Frame = 0


Query: 23  KPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVN 82
           +P+ R VW  N + E++R   CL+ + +++ DTE+PG I  T + S  ++ Y+ M+ NV 
Sbjct: 6   QPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVE 65

Query: 83  QTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIP 142
            TKLIQ G T  +  G+IGG WE NF +F   +D  N  SI FL+++GLD  +++ +G+ 
Sbjct: 66  NTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVD 125

Query: 143 L--VTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVG 202
           +    F    + + R  +   +VTF G YD  Y +  +    +LPE+  EF   VV   G
Sbjct: 126 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFG 185

Query: 203 SVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFEP 262
            V D K +A FCEGL +  LGL +L +LL+  R+G  HHAGSDSL+TA +  K+   +E 
Sbjct: 186 QVYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE- 245

Query: 263 GSEICDGFLYGL 273
            S    G +YG+
Sbjct: 246 DSRFARGLIYGI 254

[Close]

BLAST of Spg001307 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.6e-46
Identity = 106/265 (40.00%), Postives = 151/265 (56.98%), Query Frame = 0


Query: 25  MIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQT 84
           M  EVW WN ++E++    CL+    ++IDTEFPG ++ TP  + DE  Y++M+ NV+ T
Sbjct: 1   MSGEVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNT 60

Query: 85  KLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLV 144
            LIQLGLT   +   I   WE N  DF+      N  SI+FLK NGLD D+++ +GI + 
Sbjct: 61  HLIQLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIE 120

Query: 145 TFTANFVQIL-RKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-V 204
            F   F QIL  K+   RWVTF G YD  YL+K +   + LPE+ +EF   V   +G  V
Sbjct: 121 EFFMEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFV 180

Query: 205 RDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIM-----ER 264
            D+K +A  C GL   + GL+R+ ++L+ +R+G  HHAGSDS LTA + TK++      R
Sbjct: 181 YDVKKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLIFDLVNSR 240

Query: 265 FEPGSEICDGFLYGLSNKLQNMEMM 283
            E      D   Y L  +    ++M
Sbjct: 241 KESTGRRADDQQYQLEQQQHQQQLM 261

[Close]

BLAST of Spg001307 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-46
Identity = 101/229 (44.10%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 28  EVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLI 87
           EVW WN E E++    CL+  + ++IDTEFPG ++ TP  + +E  Y++M+ NV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 88  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFT 147
           QLG T  D  G     WE N  DFD      N  SI+FLK NGL+ D+++ +GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 148 ANFVQIL-RKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-VRDL 207
            +F QIL  K+    WV F G YD  YLVK +   + LPE+ EEF   V   +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 208 KHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKI 255
           K IA  C GL   + GL+R+ ++L+ KR+G  HHAGSDS LTA + TK+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Spg001307 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 97/222 (43.69%), Postives = 135/222 (60.81%), Query Frame = 0


Query: 38  ISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLIQLGLTASDEC 97
           +S  + CL SY  ++IDTEFP  ++ T   + DE+ Y +M  +V++ KLIQLGLT  D  
Sbjct: 1   MSLIEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDIN 60

Query: 98  GKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFTANFVQILRK- 157
           G+IGG WE NF DF  + DA N  SI FL++NGLD  +++ +GI +  F +    +L+K 
Sbjct: 61  GRIGGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKT 120

Query: 158 NQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGSVRDLKHIARFCEGLE 217
            +   WVTFHG YDI YL+K     + LP + E F   V   +GSV DLK +A  CEGL 
Sbjct: 121 RRNITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL- 180

Query: 218 DGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERF 259
             +LGLE L       R+G+ HHAGS++ LTA +  K++  F
Sbjct: 181 SSRLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

[Close]

BLAST of Spg001307 vs. ExPASy TrEMBL
Match: A0A6J1CXP9 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 4.4e-103
Identity = 186/301 (61.79%), Postives = 227/301 (75.42%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M R  R   RR    IREVW+ NF+ E++  + CL  Y IL+IDTEFPGFI++TPWG+I+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           E++Y + R NVN+TKLIQLGLTASDE G IG  WEFNF DFD + D HN SS+SFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LDFD+LK  GIP+  FT+    + RK++I RWVTFHGLYDIGYL+KAMGV  E+PESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAAL 250
           F  LV  A+G+V DLKH+A+FCEGLEDGKLGLE+L +L+ QKR G+KHHAGSDSLLTA+L
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 251 HTKIMERFEPGSEICDGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSIPVYYFPCY--Y 310
           + K+  +FE GSE+CDGFLYGL  KL      K+K  Q + FG+IP  +   Y PCY  Y
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLH----AKIKLYQNLMFGQIPYGLRACYPPCYIAY 297

[Close]

BLAST of Spg001307 vs. ExPASy TrEMBL
Match: A0A6J1G7L2 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 2.4e-101
Identity = 180/269 (66.91%), Postives = 215/269 (79.93%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPG I++TPWGSID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           E++Y+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSI+FL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LDF +LK  GIPL  FT NF +I+R++++ +WVTFHGLYDIGYLVKAMGV +ELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAAL 250
           F  +V   VG VRDLK +AR+CEGLE G++GLERLGELL +KR G KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 251 HTKIMERFEPGSEICDGFLYGLSNKLQNM 280
           H K+   F   SE  DG LYGL NKLQ M
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYM 269

[Close]

BLAST of Spg001307 vs. ExPASy TrEMBL
Match: A0A0A0LPP0 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G270190 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 7.8e-100
Identity = 182/302 (60.26%), Postives = 230/302 (76.16%), Query Frame = 0


Query: 11  MSRENRWGPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSID 70
           M   N+    R KP+IR+VWA NF+SEI+RFD CL  +TIL+IDTEFPGFI  +P GSID
Sbjct: 1   MGGRNQTRSTREKPLIRQVWASNFDSEIARFDECLRFHTILTIDTEFPGFIAQSPRGSID 60

Query: 71  EQIYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 130
           +++Y++   NVNQTKLIQLG+TASD+ G+IGG+WEFNF DFD E DAH+P +I FL+ NG
Sbjct: 61  DELYKDFCFNVNQTKLIQLGITASDDLGQIGGSWEFNFSDFDFEADAHSPYAIPFLEHNG 120

Query: 131 LDFDRLKSDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEE 190
           LD  ++K DGIP+ +FT  F+ ILRK  IFRWVTFHGLYDIGYL+KAMG++  LP+SMEE
Sbjct: 121 LDLKKMKKDGIPIASFTKKFLPILRKRDIFRWVTFHGLYDIGYLIKAMGLITVLPKSMEE 180

Query: 191 FGNLVVNAVGSVRDLKHIARFCEGLED-GKLGLERLGELLEQKRLGSKHHAGSDSLLTAA 250
           F  +VVN VG VRDLKH+A+FCEGL+D G+LGLERLG+LL  KR G KH+AGSDSLLTA+
Sbjct: 181 FATVVVNEVGIVRDLKHMAKFCEGLDDHGRLGLERLGKLLNLKRFGMKHNAGSDSLLTAS 240

Query: 251 LHTKIMERFEPGSEICDGFLYGLSNKLQNMEM-MKMKAV--QTITFGRIPVSIPVYYFPC 309
            H +++ERF   S++C+GFLYG S  L++M+M MKMK      + F          Y PC
Sbjct: 241 AHLEMVERFGMNSKVCNGFLYGFSETLESMKMKMKMKICHHNILRFS--------LYIPC 294

[Close]

BLAST of Spg001307 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.7e-99
Identity = 172/262 (65.65%), Postives = 209/262 (79.77%), Query Frame = 0


Query: 18  GPRRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNM 77
           GPR   P+IREVWA+N + EI R DRCL SY+I+SIDTEFPG I+ TPWG+ DE++Y + 
Sbjct: 7   GPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDEELYADF 66

Query: 78  RVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLK 137
           R NVNQTK+IQLG+T SDE G IGG WEFNF DFD E DAHNP+SI FLKQNGLDF +LK
Sbjct: 67  RFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLK 126

Query: 138 SDGIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVN 197
            DGI +  F   F+  L K+ I+RWVTFHGLYDIGYL+ AMGVV+ LPE++ EF  +V  
Sbjct: 127 KDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFKWIVAR 186

Query: 198 AVGSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMER 257
            VG+VRDLKH+ARFCEGLE G LGLE+LG+LL+QKR G KHHAGSDSL+TA LH K+++R
Sbjct: 187 RVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQR 246

Query: 258 FEPGSEICDGFLYGLSNKLQNM 280
           ++  +EICDGFLYGLS K + +
Sbjct: 247 YDFHAEICDGFLYGLSKKFEEL 268

[Close]

BLAST of Spg001307 vs. ExPASy TrEMBL
Match: A0A5A7TRG1 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001200 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.5e-98
Identity = 170/263 (64.64%), Postives = 217/263 (82.51%), Query Frame = 0


Query: 20  RRRKPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRV 79
           R +KP IR+VWA NF SEI++FD CL  +TIL+IDTEFPGFI  +P  SIDE+IY+N R 
Sbjct: 11  RSKKPFIRQVWASNFNSEITQFDECLRFHTILTIDTEFPGFIIQSPRDSIDEEIYKNFRF 70

Query: 80  NVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSD 139
           NVNQ KLIQLG+TAS++ G+IGG+WEFNF DFD + D+H+PS+I FL++NGLDF +LK  
Sbjct: 71  NVNQMKLIQLGITASNDFGQIGGSWEFNFSDFDFQVDSHSPSAIPFLEKNGLDFKKLKKY 130

Query: 140 GIPLVTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAV 199
           GIP+ +FT  F+ I++K  IFRWVTFHGLYDIGYL+KAMG++  LP+SMEEF  LVVN V
Sbjct: 131 GIPIASFTKKFLPIIQKRDIFRWVTFHGLYDIGYLIKAMGLITVLPKSMEEFATLVVNEV 190

Query: 200 GSVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFE 259
           G VRDLKH+ARFCEGLEDG+LGLERLG++L +KR G KH+AGSDSLLTA+ H ++++RF 
Sbjct: 191 GIVRDLKHMARFCEGLEDGRLGLERLGKILNKKRFGMKHNAGSDSLLTASAHFEMVKRFG 250

Query: 260 PGSEICDGFLYGLSNKLQNMEMM 283
              E+C+GFLYG S  L++M+M+
Sbjct: 251 MTYEVCNGFLYGFSESLESMKMI 273

[Close]

BLAST of Spg001307 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.1e-48
Identity = 108/276 (39.13%), Postives = 155/276 (56.16%), Query Frame = 0


Query: 28  EVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLI 87
           EVW WN E E+     CL+ ++ ++IDTEFPG ++ TP  + +E  Y++M+ NV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 88  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFT 147
           QLG T  D  G I   WE N  DF+      N  SI+FLK NGL+ D++  +GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 148 ANFVQILR-KNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-VRDL 207
            +F QIL+ K+    WV F G YD  YLVK +   + LPE+ EEF   V   +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 208 KHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFEPGSEIC 267
           K IA  C GL   + GL+R+ ++L+ KR+G  HHAGSDS LTA + TK+        + C
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKLTFDLLNSRKQC 242

Query: 268 DGFLYGLSNKLQNMEMMKMKAVQTITFGRIPVSIPV 302
              +     +L+  ++M  +         IP+ IP+
Sbjct: 243 VRRVDHQQYQLEQQQLMMTRCY-------IPIPIPI 269

[Close]

BLAST of Spg001307 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.0e-48
Identity = 97/252 (38.49%), Postives = 146/252 (57.94%), Query Frame = 0


Query: 23  KPMIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVN 82
           +P+ R VW  N + E++R   CL+ + +++ DTE+PG I  T + S  ++ Y+ M+ NV 
Sbjct: 6   QPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVE 65

Query: 83  QTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIP 142
            TKLIQ G T  +  G+IGG WE NF +F   +D  N  SI FL+++GLD  +++ +G+ 
Sbjct: 66  NTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVD 125

Query: 143 L--VTFTANFVQILRKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVG 202
           +    F    + + R  +   +VTF G YD  Y +  +    +LPE+  EF   VV   G
Sbjct: 126 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFG 185

Query: 203 SVRDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERFEP 262
            V D K +A FCEGL +  LGL +L +LL+  R+G  HHAGSDSL+TA +  K+   +E 
Sbjct: 186 QVYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE- 245

Query: 263 GSEICDGFLYGL 273
            S    G +YG+
Sbjct: 246 DSRFARGLIYGI 254

[Close]

BLAST of Spg001307 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 106/265 (40.00%), Postives = 151/265 (56.98%), Query Frame = 0


Query: 25  MIREVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQT 84
           M  EVW WN ++E++    CL+    ++IDTEFPG ++ TP  + DE  Y++M+ NV+ T
Sbjct: 1   MSGEVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNT 60

Query: 85  KLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLV 144
            LIQLGLT   +   I   WE N  DF+      N  SI+FLK NGLD D+++ +GI + 
Sbjct: 61  HLIQLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIE 120

Query: 145 TFTANFVQIL-RKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-V 204
            F   F QIL  K+   RWVTF G YD  YL+K +   + LPE+ +EF   V   +G  V
Sbjct: 121 EFFMEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFV 180

Query: 205 RDLKHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIM-----ER 264
            D+K +A  C GL   + GL+R+ ++L+ +R+G  HHAGSDS LTA + TK++      R
Sbjct: 181 YDVKKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLIFDLVNSR 240

Query: 265 FEPGSEICDGFLYGLSNKLQNMEMM 283
            E      D   Y L  +    ++M
Sbjct: 241 KESTGRRADDQQYQLEQQQHQQQLM 261

[Close]

BLAST of Spg001307 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-47
Identity = 101/229 (44.10%), Postives = 138/229 (60.26%), Query Frame = 0


Query: 28  EVWAWNFESEISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLI 87
           EVW WN E E++    CL+  + ++IDTEFPG ++ TP  + +E  Y++M+ NV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 88  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFT 147
           QLG T  D  G     WE N  DFD      N  SI+FLK NGL+ D+++ +GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 148 ANFVQIL-RKNQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGS-VRDL 207
            +F QIL  K+    WV F G YD  YLVK +   + LPE+ EEF   V   +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 208 KHIARFCEGLEDGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKI 255
           K IA  C GL   + GL+R+ ++L+ KR+G  HHAGSDS LTA + TK+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Spg001307 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 8.4e-46
Identity = 97/222 (43.69%), Postives = 135/222 (60.81%), Query Frame = 0


Query: 38  ISRFDRCLESYTILSIDTEFPGFIQTTPWGSIDEQIYQNMRVNVNQTKLIQLGLTASDEC 97
           +S  + CL SY  ++IDTEFP  ++ T   + DE+ Y +M  +V++ KLIQLGLT  D  
Sbjct: 1   MSLIEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDIN 60

Query: 98  GKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRLKSDGIPLVTFTANFVQILRK- 157
           G+IGG WE NF DF  + DA N  SI FL++NGLD  +++ +GI +  F +    +L+K 
Sbjct: 61  GRIGGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKT 120

Query: 158 NQIFRWVTFHGLYDIGYLVKAMGVVQELPESMEEFGNLVVNAVGSVRDLKHIARFCEGLE 217
            +   WVTFHG YDI YL+K     + LP + E F   V   +GSV DLK +A  CEGL 
Sbjct: 121 RRNITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL- 180

Query: 218 DGKLGLERLGELLEQKRLGSKHHAGSDSLLTAALHTKIMERF 259
             +LGLE L       R+G+ HHAGS++ LTA +  K++  F
Sbjct: 181 SSRLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

The following BLAST results are available for this feature:

BLAST of Spg001307 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895744.1	1.7e-109	66.33	putative CCR4-associated factor 1 homolog 8 [Benincasa hispida]	[more]
KAG6604931.1	9.1e-103	64.71	putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp....	[more]
XP_022146113.1	9.1e-103	61.79	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022947867.1	5.0e-101	66.91	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
KAG6604930.1	1.5e-100	65.65	putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Spg001307 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C6M9	4.3e-47	39.13	Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	5.7e-47	38.49	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
O64773	1.6e-46	40.00	Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SFX6	2.2e-46	44.10	Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM4	1.2e-44	43.69	Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of Spg001307 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXP9	4.4e-103	61.79	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=...	[more]
A0A6J1G7L2	2.4e-101	66.91	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3...	[more]
A0A0A0LPP0	7.8e-100	60.26	Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G270190 PE=3 SV...	[more]
A0A6J1G7T4	1.7e-99	65.65	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3...	[more]
A0A5A7TRG1	1.5e-98	64.64	Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Spg001307 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27890.1	3.1e-48	39.13	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G06450.1	4.0e-48	38.49	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G61470.1	1.2e-47	40.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G27820.1	1.5e-47	44.10	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G44240.1	8.4e-46	43.69	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 15..276 e-value: 1.9E-70 score: 239.0
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 31..144 e-value: 1.5E-11 score: 44.2
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 24..295
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 24..295
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 20..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg001307.1	Spg001307.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity