Spg001281 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001281
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold8: 46440291 .. 46441514 (-)
RNA-Seq ExpressionSpg001281
SyntenySpg001281
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGGAAGACGATGGAATTGAAATCCCTTCCTATTTCCTTTGCCCAATTTCCCTCCAACTCATGAGAGATCCAGTCACAATCTCCACCGGCATCACTTACGATCGACCCAACATCGAAAAATGGCTCTTTTCATGCAACAAGAAAACTTGCCCCATTACCAAACAGCCCTTGTCTGATCCCGATCTGCTTACTCCAAATCACACTCTTCGCCGTTTGATCCAAGCTTGGTGCACTCTCAATGCTTCTCATGGAGTTGAAAGAATTCCAACTCCAAAGTCACCGATCGACAAAACCCATGTCGCCAAAATCCTCAAAGAGGCCAAAAAGTTCCCCAATTCCAACCATAAATGCCTTCTTGCACTTAAAGCCGTCGCCTTGGAGAGTGAAAGAAACCGCAACCTTGTCGCTGAATCCGATGGAGCATTTGACTTTCTAGCTGCCGTTGTAAAGAGTGGTAATGGCAATATCGATTCGATTGAATTAGCGGTTGAAATTCTTTTTCACCTTAAATCCTCCGAACCCCATCTCAAGAATCTTGTGAATGGCGATGGTAACTTCTTGAACTCTTTCACTTTCGTCCTCCGACATGGGAGCTGCCAATCTAGAGCCTATGCGGTGATGTTGCTCAAGTCAAGCTTGGAACTTGCCGATCCGATTCGGTTGATGAGTGTTGAGAGGGAGCTTTTGAGTGAAATTGTGATGGTGGTTCATGATCAGATATCTCACCAAGCCTCCAAATCAGCGTTGAAGGTTTTGGCGGAGGTCTGTCCGTGGGGGCGGAACCGTGTCAAGGCGGTGGAGGGTGGCGCAGTTGGGGTTTTGGTGGAGTTGCTTTTGAACTCGGCCGAGAGGCGGTGGTCGGAGCTAGGGTTGGTGGTTTTGGATCAGCTTTGCGGTTGCGCGGAAGGACGGGAGAAGTTGATGGAACACGGGGCTGGGGTTGCAATCGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGTGATCGAGCAGTGAGGATATTGTCATCGATATGTCGGTTTTCGGCGTCGGTGAAAGTAGTTCAAGAAATGTTGGAGGTTGGGGTGGTGGCCAAGTTGTGTTTGGTGTTGCAAATGGATTGCAGTTTGAAGACAAGGGAGAAGGCAAGGGAGATACTCAAGTTGCATTCTAGGGTTTGGAGTAACTCTTCTTGCATTCCTCCACATTTGTTGTCTGCCTATCCATCATCTTGA

mRNA sequence

ATGAAAATGGAAGACGATGGAATTGAAATCCCTTCCTATTTCCTTTGCCCAATTTCCCTCCAACTCATGAGAGATCCAGTCACAATCTCCACCGGCATCACTTACGATCGACCCAACATCGAAAAATGGCTCTTTTCATGCAACAAGAAAACTTGCCCCATTACCAAACAGCCCTTGTCTGATCCCGATCTGCTTACTCCAAATCACACTCTTCGCCGTTTGATCCAAGCTTGGTGCACTCTCAATGCTTCTCATGGAGTTGAAAGAATTCCAACTCCAAAGTCACCGATCGACAAAACCCATGTCGCCAAAATCCTCAAAGAGGCCAAAAAGTTCCCCAATTCCAACCATAAATGCCTTCTTGCACTTAAAGCCGTCGCCTTGGAGAGTGAAAGAAACCGCAACCTTGTCGCTGAATCCGATGGAGCATTTGACTTTCTAGCTGCCGTTGTAAAGAGTGGTAATGGCAATATCGATTCGATTGAATTAGCGGTTGAAATTCTTTTTCACCTTAAATCCTCCGAACCCCATCTCAAGAATCTTGTGAATGGCGATGGTAACTTCTTGAACTCTTTCACTTTCGTCCTCCGACATGGGAGCTGCCAATCTAGAGCCTATGCGGTGATGTTGCTCAAGTCAAGCTTGGAACTTGCCGATCCGATTCGGTTGATGAGTGTTGAGAGGGAGCTTTTGAGTGAAATTGTGATGGTGGTTCATGATCAGATATCTCACCAAGCCTCCAAATCAGCGTTGAAGGTTTTGGCGGAGGTCTGTCCGTGGGGGCGGAACCGTGTCAAGGCGGTGGAGGGTGGCGCAGTTGGGGTTTTGGTGGAGTTGCTTTTGAACTCGGCCGAGAGGCGGTGGTCGGAGCTAGGGTTGGTGGTTTTGGATCAGCTTTGCGGTTGCGCGGAAGGACGGGAGAAGTTGATGGAACACGGGGCTGGGGTTGCAATCGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGTGATCGAGCAGTGAGGATATTGTCATCGATATGTCGGTTTTCGGCGTCGGTGAAAGTAGTTCAAGAAATGTTGGAGGTTGGGGTGGTGGCCAAGTTGTGTTTGGTGTTGCAAATGGATTGCAGTTTGAAGACAAGGGAGAAGGCAAGGGAGATACTCAAGTTGCATTCTAGGGTTTGGAGTAACTCTTCTTGCATTCCTCCACATTTGTTGTCTGCCTATCCATCATCTTGA

Coding sequence (CDS)

ATGAAAATGGAAGACGATGGAATTGAAATCCCTTCCTATTTCCTTTGCCCAATTTCCCTCCAACTCATGAGAGATCCAGTCACAATCTCCACCGGCATCACTTACGATCGACCCAACATCGAAAAATGGCTCTTTTCATGCAACAAGAAAACTTGCCCCATTACCAAACAGCCCTTGTCTGATCCCGATCTGCTTACTCCAAATCACACTCTTCGCCGTTTGATCCAAGCTTGGTGCACTCTCAATGCTTCTCATGGAGTTGAAAGAATTCCAACTCCAAAGTCACCGATCGACAAAACCCATGTCGCCAAAATCCTCAAAGAGGCCAAAAAGTTCCCCAATTCCAACCATAAATGCCTTCTTGCACTTAAAGCCGTCGCCTTGGAGAGTGAAAGAAACCGCAACCTTGTCGCTGAATCCGATGGAGCATTTGACTTTCTAGCTGCCGTTGTAAAGAGTGGTAATGGCAATATCGATTCGATTGAATTAGCGGTTGAAATTCTTTTTCACCTTAAATCCTCCGAACCCCATCTCAAGAATCTTGTGAATGGCGATGGTAACTTCTTGAACTCTTTCACTTTCGTCCTCCGACATGGGAGCTGCCAATCTAGAGCCTATGCGGTGATGTTGCTCAAGTCAAGCTTGGAACTTGCCGATCCGATTCGGTTGATGAGTGTTGAGAGGGAGCTTTTGAGTGAAATTGTGATGGTGGTTCATGATCAGATATCTCACCAAGCCTCCAAATCAGCGTTGAAGGTTTTGGCGGAGGTCTGTCCGTGGGGGCGGAACCGTGTCAAGGCGGTGGAGGGTGGCGCAGTTGGGGTTTTGGTGGAGTTGCTTTTGAACTCGGCCGAGAGGCGGTGGTCGGAGCTAGGGTTGGTGGTTTTGGATCAGCTTTGCGGTTGCGCGGAAGGACGGGAGAAGTTGATGGAACACGGGGCTGGGGTTGCAATCGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGTGATCGAGCAGTGAGGATATTGTCATCGATATGTCGGTTTTCGGCGTCGGTGAAAGTAGTTCAAGAAATGTTGGAGGTTGGGGTGGTGGCCAAGTTGTGTTTGGTGTTGCAAATGGATTGCAGTTTGAAGACAAGGGAGAAGGCAAGGGAGATACTCAAGTTGCATTCTAGGGTTTGGAGTAACTCTTCTTGCATTCCTCCACATTTGTTGTCTGCCTATCCATCATCTTGA

Protein sequence

MKMEDDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLALKAVALESERNRNLVAESDGAFDFLAAVVKSGNGNIDSIELAVEILFHLKSSEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS
Homology
BLAST of Spg001281 vs. NCBI nr
Match: XP_023513225.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 728.8 bits (1880), Expect = 2.6e-206
Identity = 368/411 (89.54%), Postives = 393/411 (95.62%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILK+AK FP+SNH
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G+ NI SIELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+ERELLSEIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNS ERRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEH AGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVWSNSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. NCBI nr
Match: XP_022944305.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 727.2 bits (1876), Expect = 7.6e-206
Identity = 364/411 (88.56%), Postives = 393/411 (95.62%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILK+AK FPNSNH
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPNSNH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G+ NI S+ELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSVELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+EREL+SEIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELVSEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALK+LAEVCPWGRNRVKAVEGGAVGVLVELLLNS +RRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKILAEVCPWGRNRVKAVEGGAVGVLVELLLNSTDRRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSAS K+VQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASKKIVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVWSNSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. NCBI nr
Match: KAG6570464.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010330.1 E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 724.9 bits (1870), Expect = 3.8e-205
Identity = 364/411 (88.56%), Postives = 392/411 (95.38%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILK+AK FPNSNH
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPNSNH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G+ NI S+ELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSVELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+ERELL+EIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLTEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNS ERRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEH AGVAIVSKKILRVSAMASDRAVRILSSICRFSAS K+VQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHDAGVAIVSKKILRVSAMASDRAVRILSSICRFSASKKIVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVW+NSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWNNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. NCBI nr
Match: XP_022985830.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 724.2 bits (1868), Expect = 6.4e-205
Identity = 366/411 (89.05%), Postives = 390/411 (94.89%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGSGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHV KILK+AK FPNS H
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVTKILKQAKNFPNSIH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G  NI SIELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGEKNIGSIELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+ERELLSEIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAV +LVELLLNS ERRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVSMLVELLLNSTERRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVWSNSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. NCBI nr
Match: XP_038902057.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 713.8 bits (1841), Expect = 8.7e-202
Identity = 364/413 (88.14%), Postives = 387/413 (93.70%), Query Frame = 0

Query: 1   MKME----DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITK 60
           MKME    D GIE+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+ CPITK
Sbjct: 1   MKMEEENNDGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRICPITK 60

Query: 61  QPLS-DPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNS 120
           QPLS DPDLLTPNHTLRRLIQ+WCTLNASHG+ERIPTPKSPIDKTHVAKILKEA+ FPNS
Sbjct: 61  QPLSPDPDLLTPNHTLRRLIQSWCTLNASHGIERIPTPKSPIDKTHVAKILKEAQNFPNS 120

Query: 121 NHKCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSS 180
           N KCLLALKA+ LESERNRNLVA+SDG F+FL  V+KS G+ NI SIE AVEILFH+K S
Sbjct: 121 NQKCLLALKAIVLESERNRNLVAQSDGVFEFLTKVIKSGGSNNIGSIESAVEILFHIKPS 180

Query: 181 EPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEI 240
           + HLKNLVNGD NF+NS TFVL++G+CQSRAYAVMLLKSSLELADPIRLM +E+ELLSEI
Sbjct: 181 QTHLKNLVNGDSNFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMGIEKELLSEI 240

Query: 241 VMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLV 300
           V V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAV VLVELLLNS+ERRWSELGLV
Sbjct: 241 VRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVQVLVELLLNSSERRWSELGLV 300

Query: 301 VLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM 360
           VLDQLCGCAEGREKL+EHGAGVA+VSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM
Sbjct: 301 VLDQLCGCAEGREKLVEHGAGVAVVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM 360

Query: 361 LEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           LEVGVVAKLCLVLQMDCSLKTREKAR+ILKLHSRVWSNSSCIPPHLLSAYPSS
Sbjct: 361 LEVGVVAKLCLVLQMDCSLKTREKARDILKLHSRVWSNSSCIPPHLLSAYPSS 413

BLAST of Spg001281 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 5.6e-119
Identity = 210/406 (51.72%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D+ IEIP +FLCPISL++M+DPV +STGITYDR +IEKWLF+  K +CP+TKQ ++D D 
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD- 66

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLALK 124
           LTPNHTLRRLIQ+WCTLNAS+GVERIPTP+ PI K+ + K+++++     +  KCL  L+
Sbjct: 67  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 126

Query: 125 AVALESERNRNLVAESDGAFDFLAAVVKSGNGNIDSIELAVEILFHLKSSEPHLKNLVNG 184
            +  E+  N+  + E+ G  +FLA +V + + N    + A+ +L+HL++SE  LKNL+N 
Sbjct: 127 QIVSENATNKRCL-EAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNN 186

Query: 185 --DGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVMVVHDQI 244
             D N + S T +++ G  +SR YA +LLK+ LE+ADP++ M+++ E+ +E+V ++ D+I
Sbjct: 187 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 246

Query: 245 SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---SAERRWSELGLVVLDQL 304
           S +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +VVLD L
Sbjct: 247 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 306

Query: 305 CGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGV 364
           C CAEGR + + HGA +A+V KKILRVS  ASDRAVR+L S+ RF A+  ++ EML++GV
Sbjct: 307 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 366

Query: 365 VAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYP 406
           VAKLCLVLQ+ C  KT+EKA+E+LKLH+RVW +S C+P +++ AYP
Sbjct: 367 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Spg001281 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.2e-118
Identity = 218/435 (50.11%), Postives = 310/435 (71.26%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D  IEIPS+FLCPISL +M+DPV +STGITYDR +IEKWLFS  K +CP+TKQ +++ D 
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD- 61

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLALK 124
           LTPNHTLRRLIQ+WCTLNAS+G+ERIPTPK PI K+ + K++KE+     +  KCL  L+
Sbjct: 62  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 121

Query: 125 AVALESERNR-------------NLVAESDGAFDFLAAVVKSGNGN-----------IDS 184
            +  E+  N+             N+V+ S   ++  ++ + S N N            DS
Sbjct: 122 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 181

Query: 185 ----IELAVEILFHLKSSEPHLKNLVNG--DGNFLNSFTFVLRHGSCQSRAYAVMLLKSS 244
               ++ A+ +L+HL +SE  LK+L+N     N + + T +++ G  +SRAYA +LLK  
Sbjct: 182 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 241

Query: 245 LELADPIRLMSVERELLSEIVMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVG 304
           LE+ADP++++ +EREL  E++ ++HDQISH+A++SA+++L   CPWGRNR KAVEGG + 
Sbjct: 242 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 301

Query: 305 VLVELLLN---SAERRWSELGLVVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMAS 364
           +++ELL++   S+ERR SE+ +VVLD LC CAEGR + + HGA +A+VSKKILRVS + S
Sbjct: 302 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 361

Query: 365 DRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWS 407
           +RAVR+L S+ RF A+  ++QEML++GVVAKLCLVLQ+ C  KT+EKA+E+LKLH+RVW 
Sbjct: 362 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 421

BLAST of Spg001281 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 6.9e-69
Identity = 166/447 (37.14%), Postives = 238/447 (53.24%), Query Frame = 0

Query: 1   MKMEDDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLS 60
           M  E++ IEIP+YF+CPISL++M+DPVT  +GITYDR NI KWL      +CP+TKQPL 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKV--PSCPVTKQPLP 60

Query: 61  DPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPN---SNH 120
               LTPNH LRRLIQ WC  N + GV RI TP+ P  K +V + +K  KKF        
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVK------SGNGNIDSIELAVEILFHL 180
           + L  L+ +A++   NR L+ E       +  VVK       G   I  ++ ++ +L  +
Sbjct: 121 ETLQKLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 180

Query: 181 KSSEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELL 240
                  K ++  +   + S T+VL      S+AY ++LL++  E      +  +  E+ 
Sbjct: 181 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIF 240

Query: 241 SEIV-----------------------------------MVVHDQISHQASKSALKVLAE 300
             I+                                    + H  +  QA  +AL +L E
Sbjct: 241 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 300

Query: 301 VCPWGRNRVKAVEGGAVGVLVELLLN-SAERRWSELGLVVLDQLCGCAEGREKLMEHGAG 360
              W RNR   V+ GAV  L+EL ++ + E+R +EL L VL +LC CA GR +++ H  G
Sbjct: 301 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 360

Query: 361 VAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSLKT 403
           +A+V+K++LRVS  A DRA+ IL+++ +FS    VV+EM+ VG V KLC VL MDC L  
Sbjct: 361 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 420

BLAST of Spg001281 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.4e-53
Identity = 139/396 (35.10%), Postives = 217/396 (54.80%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D GI+IP +F CPISL+LM+DPVT+ TG TYDR +IE W+   N  TCP+T+ PLSD  L
Sbjct: 9   DLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTL 68

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFP------NSNHK 124
           + PNHTLRRLIQ WC  N S+GVERIPTPK P D T V  +L +A           S   
Sbjct: 69  I-PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 128

Query: 125 CLLALKAVALESERNRNLVAESDGAFDFLAAVVKSGNGNIDSIELAVEILFHLKSSEPHL 184
            L  L+  A +S++NR L+A +  A + L  ++ S   + + +  ++ +L  L  +EP+ 
Sbjct: 129 ALRRLRGFARDSDKNRVLIA-AHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 188

Query: 185 KNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLK---SSLELADPIRLMSVERELLSEIV 244
              ++ D   +   T +L   S ++R  A  L++   +  + AD    +S    +   ++
Sbjct: 189 FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVL 248

Query: 245 MVVHDQI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLV 304
            ++ + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L 
Sbjct: 249 DLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALA 308

Query: 305 VLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM 364
            ++ LC   EG     EH   V ++ K ILRVS  A++ A   L ++C  +A  +  +E 
Sbjct: 309 TVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEA 368

Query: 365 LEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVW 391
              GVV +L L++Q +C+ + ++KA+++LKL    W
Sbjct: 369 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Spg001281 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 8.5e-51
Identity = 143/408 (35.05%), Postives = 216/408 (52.94%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D GI+IP +F CPISL LM DPVTISTG TYDR +I+ W+ +    TCP+T+  LSD  L
Sbjct: 9   DLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTL 68

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFP------NSNHK 124
           + PNHTLRRLIQ WC  N S+GVERIPTPK P D   V  +L +A           S   
Sbjct: 69  I-PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 128

Query: 125 CLLALKAVALESERNRNLVAESDGAFDFLAAVV------KSGNGNIDSIELAVEILFHLK 184
            +  L+ +A +SE+NR L+A    A + L  ++       S +  + S  LA+ +L H+ 
Sbjct: 129 AIRRLRGLARDSEKNRVLIA-GHNAREILVRILFADIETTSLSSELVSESLALLVLLHMT 188

Query: 185 SSEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRL---MSVERE 244
            +E      V  D + +   T +L   S + R  A  L++  L  A  + L   +S    
Sbjct: 189 ETE---CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDS 248

Query: 245 LLSEIVMVVHDQI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRW 304
           +   ++ ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  
Sbjct: 249 IFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 308

Query: 305 SELGLVVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASV 364
           +E GL  ++ LC   EG     EH   V ++ K ILRVS  A++ A   L ++C  +A  
Sbjct: 309 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEE 368

Query: 365 KVVQEMLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCI 397
           +   E    G+V +L L++Q DC+ + + KA+ +LKL    W + S +
Sbjct: 369 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

BLAST of Spg001281 vs. ExPASy TrEMBL
Match: A0A6J1FYK9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448788 PE=4 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.7e-206
Identity = 364/411 (88.56%), Postives = 393/411 (95.62%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILK+AK FPNSNH
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPNSNH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G+ NI S+ELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSVELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+EREL+SEIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELVSEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALK+LAEVCPWGRNRVKAVEGGAVGVLVELLLNS +RRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKILAEVCPWGRNRVKAVEGGAVGVLVELLLNSTDRRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSAS K+VQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASKKIVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVWSNSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. ExPASy TrEMBL
Match: A0A6J1JCD1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483760 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.1e-205
Identity = 366/411 (89.05%), Postives = 390/411 (94.89%), Query Frame = 0

Query: 1   MKMEDDG---IEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQ 60
           M+ME+DG   +E+PSYFLCPISLQLMRDPVTISTGITYDR NIEKWLFSCNK+TCPITKQ
Sbjct: 1   MRMEEDGSGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQ 60

Query: 61  PLSDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNH 120
           PLSDPDL+TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHV KILK+AK FPNS H
Sbjct: 61  PLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVTKILKQAKNFPNSIH 120

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEP 180
           KCLLALKA+ALESERN  LVA+SDGAF+F+AAV+KS G  NI SIELAVEILFH+KSSE 
Sbjct: 121 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGEKNIGSIELAVEILFHIKSSEA 180

Query: 181 HLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVM 240
           HLKNLVNGDGNF+NS TFVL++G+CQSRAYA+MLLKSSLELADPIRLMS+ERELLSEIV 
Sbjct: 181 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVR 240

Query: 241 VVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVL 300
           V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAV +LVELLLNS ERRWSE+GLV+L
Sbjct: 241 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVSMLVELLLNSTERRWSEIGLVIL 300

Query: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360
           DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE
Sbjct: 301 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 360

Query: 361 VGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           VGVVAKLCLVLQMDCS KTREKAR+ILKLHSRVWSNSSCIPPHL SAYPSS
Sbjct: 361 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of Spg001281 vs. ExPASy TrEMBL
Match: A0A1S3BME5 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491434 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.3e-199
Identity = 359/414 (86.71%), Postives = 387/414 (93.48%), Query Frame = 0

Query: 1   MKMEDD-----GIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPIT 60
           MKMED      GIE+PSYFLCPISLQLMRDPVTISTGITYDR +IEKWLFS NK  CPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPL-SDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPN 120
           KQPL SDPDLLTPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILK+A+ FPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKS 180
           SNHKCLLALKA+ALE+ERNRNLVA+SDGAFDFLA V+KS G+ NI SIELAVEILFH+KS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSE 240
           S+ HLKNLVNGD +F+NS TFVL++G+CQSRAYAVMLLKSSLELADPIRLMS+ERELL E
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 IVMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGL 300
            V V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNS ERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQE 360
           VVLDQLCGCAEGREKL+EH AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QE
Sbjct: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           MLEVGVVAKLCLVLQMDCSLKTREKAR+ LKLHSRVWSNSSCIPPHLLSAYPS+
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of Spg001281 vs. ExPASy TrEMBL
Match: A0A5D3B896 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00310 PE=4 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.7e-198
Identity = 355/406 (87.44%), Postives = 384/406 (94.58%), Query Frame = 0

Query: 4   EDDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPL-SDP 63
           E+ GIE+PSYFLCPISLQLMRDPVTISTGITYDR +IEKWLFS NK  CPITKQPL SDP
Sbjct: 7   ENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDP 66

Query: 64  DLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLA 123
           DLLTPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILK+A+ FPNSNHKCLLA
Sbjct: 67  DLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNHKCLLA 126

Query: 124 LKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKSSEPHLKNL 183
           LKA+ALE+ERNRNLVA+SDGAFDFLA V+KS G+ NI SIELAVEILFH+KSS+ HLKNL
Sbjct: 127 LKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQAHLKNL 186

Query: 184 VNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVMVVHDQ 243
           VNGD +F+NS TFVL++G+CQSRAYAVMLLKSSLELADPIRLMS+ERELL E V V+HD 
Sbjct: 187 VNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETVRVIHDH 246

Query: 244 ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLVVLDQLCG 303
           ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNS ERRWSELGLVVLDQLCG
Sbjct: 247 ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVVLDQLCG 306

Query: 304 CAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVA 363
           CAEGREKL+EH AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QEMLEVGVVA
Sbjct: 307 CAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLEVGVVA 366

Query: 364 KLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           KLCLVLQMDCSLKTREKAR+ LKLHSRVWSNSSCIPPHLLSAYPS+
Sbjct: 367 KLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of Spg001281 vs. ExPASy TrEMBL
Match: A0A5A7V391 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G001090 PE=4 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.7e-198
Identity = 358/414 (86.47%), Postives = 386/414 (93.24%), Query Frame = 0

Query: 1   MKMEDD-----GIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPIT 60
           MKMED      GIE+PSYFLCPISLQLMRDPVTISTGITYDR +IEKWLFS NK  CPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPL-SDPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPN 120
           KQPL SDPDLLTPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILK+A+ FPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAVALESERNRNLVAESDGAFDFLAAVVKS-GNGNIDSIELAVEILFHLKS 180
           SNHKCLLALKA+ALE+ERNRNLVA+SDGAFDFLA V+KS G+ NI SIELAVEILFH+KS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSE 240
           S+ HLKNLVNGD +F+NS TFVL++G+CQSRAYAVMLLKSSLELADPIRLMS+ERELL E
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 IVMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGL 300
            V V+HD ISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNS ERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQE 360
           VVLDQLC CAEGREKL+EH AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QE
Sbjct: 301 VVLDQLCECAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYPSS 408
           MLEVGVVAKLCLVLQMDCSLKTREKAR+ LKLHSRVWSNSSCIPPHLLSAYPS+
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of Spg001281 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 429.1 bits (1102), Expect = 4.0e-120
Identity = 210/406 (51.72%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D+ IEIP +FLCPISL++M+DPV +STGITYDR +IEKWLF+  K +CP+TKQ ++D D 
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD- 66

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLALK 124
           LTPNHTLRRLIQ+WCTLNAS+GVERIPTP+ PI K+ + K+++++     +  KCL  L+
Sbjct: 67  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 126

Query: 125 AVALESERNRNLVAESDGAFDFLAAVVKSGNGNIDSIELAVEILFHLKSSEPHLKNLVNG 184
            +  E+  N+  + E+ G  +FLA +V + + N    + A+ +L+HL++SE  LKNL+N 
Sbjct: 127 QIVSENATNKRCL-EAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNN 186

Query: 185 --DGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELLSEIVMVVHDQI 244
             D N + S T +++ G  +SR YA +LLK+ LE+ADP++ M+++ E+ +E+V ++ D+I
Sbjct: 187 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 246

Query: 245 SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---SAERRWSELGLVVLDQL 304
           S +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +VVLD L
Sbjct: 247 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 306

Query: 305 CGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGV 364
           C CAEGR + + HGA +A+V KKILRVS  ASDRAVR+L S+ RF A+  ++ EML++GV
Sbjct: 307 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 366

Query: 365 VAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCIPPHLLSAYP 406
           VAKLCLVLQ+ C  KT+EKA+E+LKLH+RVW +S C+P +++ AYP
Sbjct: 367 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Spg001281 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 425.6 bits (1093), Expect = 4.4e-119
Identity = 218/435 (50.11%), Postives = 310/435 (71.26%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D  IEIPS+FLCPISL +M+DPV +STGITYDR +IEKWLFS  K +CP+TKQ +++ D 
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD- 61

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPNSNHKCLLALK 124
           LTPNHTLRRLIQ+WCTLNAS+G+ERIPTPK PI K+ + K++KE+     +  KCL  L+
Sbjct: 62  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 121

Query: 125 AVALESERNR-------------NLVAESDGAFDFLAAVVKSGNGN-----------IDS 184
            +  E+  N+             N+V+ S   ++  ++ + S N N            DS
Sbjct: 122 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 181

Query: 185 ----IELAVEILFHLKSSEPHLKNLVNG--DGNFLNSFTFVLRHGSCQSRAYAVMLLKSS 244
               ++ A+ +L+HL +SE  LK+L+N     N + + T +++ G  +SRAYA +LLK  
Sbjct: 182 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 241

Query: 245 LELADPIRLMSVERELLSEIVMVVHDQISHQASKSALKVLAEVCPWGRNRVKAVEGGAVG 304
           LE+ADP++++ +EREL  E++ ++HDQISH+A++SA+++L   CPWGRNR KAVEGG + 
Sbjct: 242 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 301

Query: 305 VLVELLLN---SAERRWSELGLVVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMAS 364
           +++ELL++   S+ERR SE+ +VVLD LC CAEGR + + HGA +A+VSKKILRVS + S
Sbjct: 302 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 361

Query: 365 DRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWS 407
           +RAVR+L S+ RF A+  ++QEML++GVVAKLCLVLQ+ C  KT+EKA+E+LKLH+RVW 
Sbjct: 362 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 421

BLAST of Spg001281 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 262.7 bits (670), Expect = 4.9e-70
Identity = 166/447 (37.14%), Postives = 238/447 (53.24%), Query Frame = 0

Query: 1   MKMEDDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLS 60
           M  E++ IEIP+YF+CPISL++M+DPVT  +GITYDR NI KWL      +CP+TKQPL 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKV--PSCPVTKQPLP 74

Query: 61  DPDLLTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFPN---SNH 120
               LTPNH LRRLIQ WC  N + GV RI TP+ P  K +V + +K  KKF        
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 KCLLALKAVALESERNRNLVAESDGAFDFLAAVVK------SGNGNIDSIELAVEILFHL 180
           + L  L+ +A++   NR L+ E       +  VVK       G   I  ++ ++ +L  +
Sbjct: 135 ETLQKLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 194

Query: 181 KSSEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRLMSVERELL 240
                  K ++  +   + S T+VL      S+AY ++LL++  E      +  +  E+ 
Sbjct: 195 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIF 254

Query: 241 SEIV-----------------------------------MVVHDQISHQASKSALKVLAE 300
             I+                                    + H  +  QA  +AL +L E
Sbjct: 255 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 314

Query: 301 VCPWGRNRVKAVEGGAVGVLVELLLN-SAERRWSELGLVVLDQLCGCAEGREKLMEHGAG 360
              W RNR   V+ GAV  L+EL ++ + E+R +EL L VL +LC CA GR +++ H  G
Sbjct: 315 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 374

Query: 361 VAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSLKT 403
           +A+V+K++LRVS  A DRA+ IL+++ +FS    VV+EM+ VG V KLC VL MDC L  
Sbjct: 375 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 434

BLAST of Spg001281 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-54
Identity = 139/396 (35.10%), Postives = 217/396 (54.80%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D GI+IP +F CPISL+LM+DPVT+ TG TYDR +IE W+   N  TCP+T+ PLSD  L
Sbjct: 9   DLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTL 68

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFP------NSNHK 124
           + PNHTLRRLIQ WC  N S+GVERIPTPK P D T V  +L +A           S   
Sbjct: 69  I-PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 128

Query: 125 CLLALKAVALESERNRNLVAESDGAFDFLAAVVKSGNGNIDSIELAVEILFHLKSSEPHL 184
            L  L+  A +S++NR L+A +  A + L  ++ S   + + +  ++ +L  L  +EP+ 
Sbjct: 129 ALRRLRGFARDSDKNRVLIA-AHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 188

Query: 185 KNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLK---SSLELADPIRLMSVERELLSEIV 244
              ++ D   +   T +L   S ++R  A  L++   +  + AD    +S    +   ++
Sbjct: 189 FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVL 248

Query: 245 MVVHDQI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRWSELGLV 304
            ++ + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L 
Sbjct: 249 DLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALA 308

Query: 305 VLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM 364
            ++ LC   EG     EH   V ++ K ILRVS  A++ A   L ++C  +A  +  +E 
Sbjct: 309 TVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEA 368

Query: 365 LEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVW 391
              GVV +L L++Q +C+ + ++KA+++LKL    W
Sbjct: 369 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Spg001281 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 202.6 bits (514), Expect = 6.0e-52
Identity = 143/408 (35.05%), Postives = 216/408 (52.94%), Query Frame = 0

Query: 5   DDGIEIPSYFLCPISLQLMRDPVTISTGITYDRPNIEKWLFSCNKKTCPITKQPLSDPDL 64
           D GI+IP +F CPISL LM DPVTISTG TYDR +I+ W+ +    TCP+T+  LSD  L
Sbjct: 9   DLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTL 68

Query: 65  LTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKEAKKFP------NSNHK 124
           + PNHTLRRLIQ WC  N S+GVERIPTPK P D   V  +L +A           S   
Sbjct: 69  I-PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 128

Query: 125 CLLALKAVALESERNRNLVAESDGAFDFLAAVV------KSGNGNIDSIELAVEILFHLK 184
            +  L+ +A +SE+NR L+A    A + L  ++       S +  + S  LA+ +L H+ 
Sbjct: 129 AIRRLRGLARDSEKNRVLIA-GHNAREILVRILFADIETTSLSSELVSESLALLVLLHMT 188

Query: 185 SSEPHLKNLVNGDGNFLNSFTFVLRHGSCQSRAYAVMLLKSSLELADPIRL---MSVERE 244
            +E      V  D + +   T +L   S + R  A  L++  L  A  + L   +S    
Sbjct: 189 ETE---CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDS 248

Query: 245 LLSEIVMVVHDQI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSAERRW 304
           +   ++ ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  
Sbjct: 249 IFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 308

Query: 305 SELGLVVLDQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASV 364
           +E GL  ++ LC   EG     EH   V ++ K ILRVS  A++ A   L ++C  +A  
Sbjct: 309 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEE 368

Query: 365 KVVQEMLEVGVVAKLCLVLQMDCSLKTREKAREILKLHSRVWSNSSCI 397
           +   E    G+V +L L++Q DC+ + + KA+ +LKL    W + S +
Sbjct: 369 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023513225.12.6e-20689.54E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
XP_022944305.17.6e-20688.56E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata][more]
KAG6570464.13.8e-20588.56E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022985830.16.4e-20589.05E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima][more]
XP_038902057.18.7e-20288.14E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q84TG35.6e-11951.72E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC66.2e-11850.11E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF156.9e-6937.14E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT792.4e-5335.10U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA48.5e-5135.05U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1FYK93.7e-20688.56RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448788... [more]
A0A6J1JCD13.1e-20589.05RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483760 P... [more]
A0A1S3BME54.3e-19986.71RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491434 PE=4 ... [more]
A0A5D3B8961.7e-19887.44RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7V3913.7e-19886.47RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT2G35930.14.0e-12051.72plant U-box 23 [more]
AT3G52450.14.4e-11950.11plant U-box 22 [more]
AT3G11840.14.9e-7037.14plant U-box 24 [more]
AT3G19380.11.7e-5435.10plant U-box 25 [more]
AT1G49780.16.0e-5235.05plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 13..78
e-value: 1.8E-30
score: 117.2
IPR003613U box domainPFAMPF04564U-boxcoord: 10..80
e-value: 2.4E-19
score: 69.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 9..85
score: 36.729111
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..89
e-value: 2.2E-29
score: 103.3
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 95..398
e-value: 5.4E-12
score: 47.0
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 4..405
NoneNo IPR availablePANTHERPTHR22849:SF11OS11G0573500 PROTEINcoord: 4..405
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 14..57
e-value: 3.36199E-23
score: 89.6002
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..84
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 117..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001281.1Spg001281.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity