Spg001238 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001238
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionubiquitin-conjugating enzyme E2 20
Locationscaffold8: 46952579 .. 46955186 (+)
RNA-Seq ExpressionSpg001238
SyntenySpg001238
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAAAAAAAAAAAACCCTCTATCTCTGTGGTTGAATAAAGTGGTGGCATTAAAGCAGTGACCAAAAGTAGCCGTTGGAAATTCAAAAGCAACCCAATTGGAATCTTCGCCGTCTCCGCAACGTTCACTAAAAATTTTGAATTATTTACCCCTTCAAATCAAATCCCTAATTCTTCATCTCTCTCCTTCGTTTTAAGCCCTTCGATCTCCTCTCTCCCTCTCCACTCCGCCTCTCTCTCTCTCTCTACCAACGCCTGATTGATTCTTCCTTTGGTGGAGAAATTCCTCGGATCGTTCTTCAATTCTTCGTATCCTCTGTAATTCGGATCAGAGAGAATGGCGGCCATTAATCGATACACTGACAACACGGCGCCGGCGAACCCTGTTGTTTCTCCAGCCAAGCAGTCCTTGCCACCGGCCAAAACCGCGGATTCTCAGTCCGTTCTCAAAAGGTATACACATGTCCGTCTCTTTCGTATTTGATTCGATTCGTACGTCACTGCGGCTCGATCGCGTTTCTGTGTGTGTGCAATCGTCTCTGTTTTTTGTGATTTTGGGCCATTTTTTTTACTTTAAGCTGGATGAGGTTGTTGTAGGGTTCTGGATTTAGTTTGAACTGATTCTGAGATGGATTGTTTTATGGTCTTACGTAATGATCTTTTTAGGGTTTTAGCGGTAATTTAAGCGTCTTTAGGCCCTATCCCTTTCCTTTTGCACCATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTATGGCCATATGAGGTCTATGTTGAAAGATAAACCTCGTTCTTGTGTGTGTGTTTTAAAGAAATTAGCGAAGAACAGATCTTACTGAGGACTCCGTCTGTTTCCAACTAAACTAACGACCTTTCTTCAATTGTTGTTGTGTTTTTCTGATAACAGGTTGCAGTCTGAATTAATGGCTCTAATGGTAAGACTTGCTACATTTTTTCATCTGATATTTACTTTTGGACTATCAAATTCTGAAGTATTTGTAAATGAGATTCTTAAGTTGGCCAAAAGACTCTTCTAAACTCATGTTTACGAAATTGCAGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATACAGGCTGTCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGTAATTCTATGGCGAAATCTTCAACATTGAAACGATAACCTCTCTGATTTGTCAGGACAAAAACAACCAGAAGTGAGAAATGTTTCCGCTTTTGCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGGTAACCCCCCAAAAAACACCATACTTTTTTATGGTTAGAATGGAAAATAGAATTTTCAATGAGCTTTTGGCATTTATTTGCAGAACCAAACATAAGTTCACCTCTAAACACACAGGCAGCCCAACTGTGGAGTAATCAACAAGGTGGGTTTCTACTGTAACACACTATTTTTCTTGTCTCTTTGAGTTGCCATGCGATGAAAGTTGGCTCACTCATGTTTTGTTTCTGTGTTTGCTGTGTAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCTTAGCTAGCTTGTTTTTTTAGTTTACGCGAAGAAACTAGGATTGTTGTCTCACTCTTTTTGCGCTAGAGAGGAGATTCCCAAATGAATTTTTTTTTCCCCCCTTTCCTTCAGTGTGCTCTTCATTTCTTTTACTCTCCCTGATTGTAATAGATATATGCTGTCTCCTGTTATATGTGGAAATTTCAACTCATGAGCTATATATTTTATTCTCATTTCGAAGTTCCTCTGTCATCCTGCCAAGTTCTTGAAATGGAAATTATGCAACCGAAAAAAAGAATCATGTATTCAAGAGATAGCTTAAATCATGTATTCGAGAT

mRNA sequence

ATGGCGGCCATTAATCGATACACTGACAACACGGCGCCGGCGAACCCTGTTGTTTCTCCAGCCAAGCAGTCCTTGCCACCGGCCAAAACCGCGGATTCTCAGTCCGTTCTCAAAAGGTTGCAGTCTGAATTAATGGCTCTAATGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATACAGGCTGTCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACATAAGTTCACCTCTAAACACACAGGCAGCCCAACTGTGGAGTAATCAACAAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCTTAG

Coding sequence (CDS)

ATGGCGGCCATTAATCGATACACTGACAACACGGCGCCGGCGAACCCTGTTGTTTCTCCAGCCAAGCAGTCCTTGCCACCGGCCAAAACCGCGGATTCTCAGTCCGTTCTCAAAAGGTTGCAGTCTGAATTAATGGCTCTAATGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATACAGGCTGTCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACATAAGTTCACCTCTAAACACACAGGCAGCCCAACTGTGGAGTAATCAACAAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCTTAG

Protein sequence

MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTAA
Homology
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BLAST of Spg001238 vs. NCBI nr
Match: XP_004140207.1 (ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ubiquitin-conjugating enzyme E2 20 [Cucumis melo] >KAA0061782.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa] >KGN48113.1 hypothetical protein Csa_003824 [Cucumis sativus] >TYJ96123.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 8.8e-98
Identity = 179/181 (98.90%), Postives = 181/181 (100.00%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

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BLAST of Spg001238 vs. NCBI nr
Match: XP_038900928.1 (LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 2.6e-97
Identity = 178/181 (98.34%), Postives = 180/181 (99.45%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

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BLAST of Spg001238 vs. NCBI nr
Match: XP_022957735.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035878.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQ++YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

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BLAST of Spg001238 vs. NCBI nr
Match: XP_023533942.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.6 bits (932), Expect = 9.8e-97
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQ++YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

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BLAST of Spg001238 vs. NCBI nr
Match: XP_022995155.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita maxima])

HSP 1 Score: 360.5 bits (924), Expect = 8.3e-96
Identity = 174/181 (96.13%), Postives = 178/181 (98.34%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT P+NPVVSPAKQSLPPAK ADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPSNPVVSPAKQSLPPAKAADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQ++YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

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BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 9.9e-84
Identity = 153/180 (85.00%), Postives = 158/180 (87.78%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

[Close]
BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 2.1e-81
Identity = 150/181 (82.87%), Postives = 156/181 (86.19%), Query Frame = 0 

Query: 1   MAAINRYTDNT-APANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT A   P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQ+EYRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

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BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match: Q54XS6 (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 GN=ube2c PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.3e-53
Identity = 98/150 (65.33%), Postives = 118/150 (78.67%), Query Frame = 0 

Query: 31  ADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSF 90
           +   SV KRLQSELM LMM    GISAFP+ DNIF W GTI G+KDTV+E  E+++S  F
Sbjct: 2   SQDHSVSKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKF 61

Query: 91  PNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNIS 150
           P DYP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+VR++L+SIQSLLGEPN  
Sbjct: 62  PTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNE 121

Query: 151 SPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 181
           SPLN+ AA LWSNQ EY+K+++K Y+  T+
Sbjct: 122 SPLNSYAASLWSNQDEYKKVLDKRYQEATS 151

[Close]
BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match: Q4R9D1 (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-51
Identity = 100/168 (59.52%), Postives = 120/168 (71.43%), Query Frame = 0 

Query: 8   TDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  PA   V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPAATSVTAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLY 176
           S+ YDVRTILLSIQSLLGEPNI SPLNT AA+LW N   ++K +++ Y
Sbjct: 123 SALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

[Close]
BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match: Q32PA5 (Ubiquitin-conjugating enzyme E2 C OS=Bos taurus OX=9913 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 4.9e-51
Identity = 99/168 (58.93%), Postives = 119/168 (70.83%), Query Frame = 0 

Query: 8   TDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  P    V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPVAASVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLY 176
           S+ YDVRTILLSIQSLLGEPNI SPLNT AA+LW N   ++K +++ Y
Sbjct: 123 SALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

[Close]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match: A0A5D3BA03 (Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00870 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.3e-98
Identity = 179/181 (98.90%), Postives = 181/181 (100.00%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match: A0A0A0KF07 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.3e-98
Identity = 179/181 (98.90%), Postives = 181/181 (100.00%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match: A0A1S3BLY8 (ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.3e-98
Identity = 179/181 (98.90%), Postives = 181/181 (100.00%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match: A0A6J1H1C8 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC111459191 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQ++YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match: A0A6J1K3C1 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111490778 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.0e-96
Identity = 174/181 (96.13%), Postives = 178/181 (98.34%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT P+NPVVSPAKQSLPPAK ADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPSNPVVSPAKQSLPPAKAADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQ++YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Spg001238 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 310.8 bits (795), Expect = 7.0e-85
Identity = 153/180 (85.00%), Postives = 158/180 (87.78%), Query Frame = 0 

Query: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ+EYRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

[Close]
BLAST of Spg001238 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 303.1 bits (775), Expect = 1.5e-82
Identity = 150/181 (82.87%), Postives = 156/181 (86.19%), Query Frame = 0 

Query: 1   MAAINRYTDNT-APANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT A   P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQQEYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQ+EYRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

[Close]
BLAST of Spg001238 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 257.7 bits (657), Expect = 7.0e-69
Identity = 122/136 (89.71%), Postives = 125/136 (91.91%), Query Frame = 0 

Query: 45  MALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFE 104
           M LMM  D GISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFE
Sbjct: 1   MGLMMGADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFE 60

Query: 105 TGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ 164
           T CFHPNVD+YGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQ
Sbjct: 61  TCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQ 120

Query: 165 QEYRKMVEKLYKPPTA 181
           +EYRKMVEKLYKP  A
Sbjct: 121 EEYRKMVEKLYKPLNA 136

[Close]
BLAST of Spg001238 vs. TAIR 10
Match: AT2G02760.1 (ubiquiting-conjugating enzyme 2 )

HSP 1 Score: 131.3 bits (329), Expect = 7.6e-31
Identity = 63/138 (45.65%), Postives = 91/138 (65.94%), Query Frame = 0 

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQQEYRKMVEKL 175
           A+++S +++EY + V ++
Sbjct: 127 ARMFSESKREYNRRVREV 144

[Close]
BLAST of Spg001238 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 131.0 bits (328), Expect = 9.9e-31
Identity = 63/135 (46.67%), Postives = 89/135 (65.93%), Query Frame = 0 

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQQEYRKMV 172
           A+++S +++EY + V
Sbjct: 127 ARMYSESKREYNRRV 141

The following BLAST results are available for this feature:
BLAST of Spg001238 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004140207.18.8e-9898.90ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ... [more]
XP_038900928.12.6e-9798.34LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida][more]
XP_022957735.17.5e-9797.79ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiqu... [more]
XP_023533942.19.8e-9797.24ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo][more]
XP_022995155.18.3e-9696.13ubiquitin-conjugating enzyme E2 20-like [Cucurbita maxima][more]
BLAST of Spg001238 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L7T39.9e-8485.00Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2... [more]
Q9LJZ52.1e-8182.87Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1... [more]
Q54XS65.3e-5365.33Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 ... [more]
Q4R9D11.7e-5159.52Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 S... [more]
Q32PA54.9e-5158.93Ubiquitin-conjugating enzyme E2 C OS=Bos taurus OX=9913 GN=UBE2C PE=2 SV=1[more]
BLAST of Spg001238 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BA034.3e-9898.90Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa O... [more]
A0A0A0KF074.3e-9898.90UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE... [more]
A0A1S3BLY84.3e-9898.90ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 ... [more]
A0A6J1H1C83.6e-9797.79ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K3C14.0e-9696.13ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111490... [more]
BLAST of Spg001238 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50490.17.0e-8585.00ubiquitin-conjugating enzyme 20 [more]
AT3G20060.11.5e-8282.87ubiquitin-conjugating enzyme19 [more]
AT3G20060.27.0e-6989.71ubiquitin-conjugating enzyme19 [more]
AT2G02760.17.6e-3145.65ubiquiting-conjugating enzyme 2 [more]
AT1G14400.19.9e-3146.67ubiquitin carrier protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 38..180
e-value: 8.3E-60
score: 214.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR24068:SF373BNAC06G03670D PROTEINcoord: 18..178
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 18..178
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 28..177
e-value: 1.6E-57
score: 195.5
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 28..174
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 39..173
e-value: 2.7E-47
score: 159.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 35..180
score: 48.463669
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 38..175
e-value: 1.06769E-64
score: 193.573
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 108..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001238.1Spg001238.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity