Spg001227 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001227
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncytochrome P450 89A2-like
Locationscaffold8: 34414012 .. 34414866 (-)
RNA-Seq ExpressionSpg001227
SyntenySpg001227
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTTTGAACTCAGAAGGAATCAAGATAAAGTCCTCATCCCTTTGATCGAAGCAAGAAGGAAGGCCAACCAAAACAGAGCAAACAGAGTAAACAGAGCAGAAGGAGAAGAAGAGGATGAAGAATTTGTTTTGTCATATGTGGATACGCTTCTCGATTTGGAGCTCCCCGACGAGAACAGAAAGCTTACTGATGAAGAAATGGTCACCATAGCCTCCGAGTTCCTCAACGCCGGCACCGACACCACCTCCACGGCCCTGCAGTGGATGATGGCGAATTTAGTGAAATACCCAGAAATTCAAAACAAACTTTTCGCAGAGATCAAAGGAGTAATGGGGGATAGATCAGGGGAGGAAGAGGTGAAGGAAGAGGATTTGGGGAAACTTCCATATTTGAAAGCTTTGGTTTTGGAAGGATTGCGAAGACACCCACCAGCGCATTTCGTGTTACCACACGCAGTGAAAGAAGATACGAATCTTGAAAACTATGTGATACCAAAGAATGGAACTGTGAATTTCATGGTGGCGGAGATGGGTTGGGATCCGGAACTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCAAAGAAGAAGAAGAAGAAGTGGCGTTTGATATAACAGGGAGCAAAGAAATAAAGATGATGCCGTTTGGAGCAGGGAGAAGGATATGTCCTGGATTTGGTCTGGCGATTCTTCATTTGGAATATTTCATTGCAAATTTGGTATGGAGGTTTGAATGGAAAGCCTTAGACGGAGACGATGTTGATCTGTCGGAGAAGGTGGAGTTCACCGTCGTGATGGAAAAGCCTCTGAAGGCCAAAATAATTTCAAGGTTTTAA

mRNA sequence

ATGTTTTTTGAACTCAGAAGGAATCAAGATAAAGTCCTCATCCCTTTGATCGAAGCAAGAAGGAAGGCCAACCAAAACAGAGCAAACAGAGTAAACAGAGCAGAAGGAGAAGAAGAGGATGAAGAATTTGTTTTGTCATATGTGGATACGCTTCTCGATTTGGAGCTCCCCGACGAGAACAGAAAGCTTACTGATGAAGAAATGGTCACCATAGCCTCCGAGTTCCTCAACGCCGGCACCGACACCACCTCCACGGCCCTGCAGTGGATGATGGCGAATTTAGTGAAATACCCAGAAATTCAAAACAAACTTTTCGCAGAGATCAAAGGAGTAATGGGGGATAGATCAGGGGAGGAAGAGGTGAAGGAAGAGGATTTGGGGAAACTTCCATATTTGAAAGCTTTGGTTTTGGAAGGATTGCGAAGACACCCACCAGCGCATTTCGTGTTACCACACGCAGTGAAAGAAGATACGAATCTTGAAAACTATGTGATACCAAAGAATGGAACTGTGAATTTCATGGTGGCGGAGATGGGTTGGGATCCGGAACTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCAAAGAAGAAGAAGAAGAAGTGGCGTTTGATATAACAGGGAGCAAAGAAATAAAGATGATGCCGTTTGGAGCAGGGAGAAGGATATGTCCTGGATTTGGTCTGGCGATTCTTCATTTGGAATATTTCATTGCAAATTTGGTATGGAGGTTTGAATGGAAAGCCTTAGACGGAGACGATGTTGATCTGTCGGAGAAGGTGGAGTTCACCGTCGTGATGGAAAAGCCTCTGAAGGCCAAAATAATTTCAAGGTTTTAA

Coding sequence (CDS)

ATGTTTTTTGAACTCAGAAGGAATCAAGATAAAGTCCTCATCCCTTTGATCGAAGCAAGAAGGAAGGCCAACCAAAACAGAGCAAACAGAGTAAACAGAGCAGAAGGAGAAGAAGAGGATGAAGAATTTGTTTTGTCATATGTGGATACGCTTCTCGATTTGGAGCTCCCCGACGAGAACAGAAAGCTTACTGATGAAGAAATGGTCACCATAGCCTCCGAGTTCCTCAACGCCGGCACCGACACCACCTCCACGGCCCTGCAGTGGATGATGGCGAATTTAGTGAAATACCCAGAAATTCAAAACAAACTTTTCGCAGAGATCAAAGGAGTAATGGGGGATAGATCAGGGGAGGAAGAGGTGAAGGAAGAGGATTTGGGGAAACTTCCATATTTGAAAGCTTTGGTTTTGGAAGGATTGCGAAGACACCCACCAGCGCATTTCGTGTTACCACACGCAGTGAAAGAAGATACGAATCTTGAAAACTATGTGATACCAAAGAATGGAACTGTGAATTTCATGGTGGCGGAGATGGGTTGGGATCCGGAACTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCAAAGAAGAAGAAGAAGAAGTGGCGTTTGATATAACAGGGAGCAAAGAAATAAAGATGATGCCGTTTGGAGCAGGGAGAAGGATATGTCCTGGATTTGGTCTGGCGATTCTTCATTTGGAATATTTCATTGCAAATTTGGTATGGAGGTTTGAATGGAAAGCCTTAGACGGAGACGATGTTGATCTGTCGGAGAAGGTGGAGTTCACCGTCGTGATGGAAAAGCCTCTGAAGGCCAAAATAATTTCAAGGTTTTAA

Protein sequence

MFFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISRF
Homology
BLAST of Spg001227 vs. NCBI nr
Match: KAG7010997.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 490.0 bits (1260), Expect = 1.4e-134
Identity = 249/292 (85.27%), Postives = 265/292 (90.75%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQ---------NRANRVNRAEGEEEDEEFVLSYVDTLL 61
           F EL+RN+D+V IPLIEARRKA Q         NR NR NRAE EEE EEFV+SYVDTLL
Sbjct: 237 FHELKRNRDEVTIPLIEARRKAIQNRENRENRANRENRANRAEAEEEKEEFVVSYVDTLL 296

Query: 62  DLELPDENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVM 121
           DLE PDE RKLTDEEM T+ASEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFAE+KGVM
Sbjct: 297 DLEHPDEKRKLTDEEMSTLASEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVM 356

Query: 122 GDRSGEEEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVN 181
           GD +G EEVKEEDLGKLPYL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNGTVN
Sbjct: 357 GDGTG-EEVKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVN 416

Query: 182 FMVAEMGWDPELWEDPMAFKPERFMKGGKEEEEEVA-FDITGSKEIKMMPFGAGRRICPG 241
           FMVAEMGWDPE+WEDPMAFKPERFMKGG+EEEEEVA FDITGSKEIKMMPFGAGRR+CPG
Sbjct: 417 FMVAEMGWDPEVWEDPMAFKPERFMKGGEEEEEEVAGFDITGSKEIKMMPFGAGRRMCPG 476

Query: 242 FGLAILHLEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           FGLAILHLEYFIANLVWRFEWKA++GD VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 FGLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPR 527

BLAST of Spg001227 vs. NCBI nr
Match: KAG6571189.1 (Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.8 bits (1257), Expect = 3.2e-134
Identity = 246/291 (84.54%), Postives = 262/291 (90.03%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQ---------NRANRVNRAEGEEEDEEFVLSYVDTLL 61
           F EL+RN+D+V IPLIEARRKA Q         NR NR NRAE EEE EEFV+SYVDTLL
Sbjct: 180 FHELKRNRDEVTIPLIEARRKAIQNRENRENRANRENRANRAEAEEEKEEFVVSYVDTLL 239

Query: 62  DLELPDENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVM 121
           DLE PDE RKLTDEEM T+ASEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFAE+KGVM
Sbjct: 240 DLEHPDEKRKLTDEEMSTLASEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVM 299

Query: 122 GDRSGEEEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVN 181
           GD +G EEVKEEDLGKLPYLKA+VLEGLRRHPP HFVLPHAVKE+T LENYVIPKNGTVN
Sbjct: 300 GDGTG-EEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKEETMLENYVIPKNGTVN 359

Query: 182 FMVAEMGWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGF 241
           FMVAEMGWDPE+WEDPMAFKPERFMKGG+EEEE   FDITGSKEIKMMPFGAGRR+CPGF
Sbjct: 360 FMVAEMGWDPEVWEDPMAFKPERFMKGGEEEEEVAGFDITGSKEIKMMPFGAGRRMCPGF 419

Query: 242 GLAILHLEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           GLAILHLEYFIANLVWRFEWKA++GD VDLSEKVEFTVVMEKPLKA II R
Sbjct: 420 GLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPR 469

BLAST of Spg001227 vs. NCBI nr
Match: XP_022944210.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 486.9 bits (1252), Expect = 1.2e-133
Identity = 245/285 (85.96%), Postives = 261/285 (91.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKA---NQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPD 61
           F EL+RN+D+V IPLIEARRKA    +NR NR NRAE EEE EEFV+SYVDTLLDLE PD
Sbjct: 237 FHELKRNRDEVTIPLIEARRKAIQNRENRENRANRAEAEEEKEEFVVSYVDTLLDLEHPD 296

Query: 62  ENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGE 121
           E RKLTDEEM T+ASEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFAE+KGVMGD +G 
Sbjct: 297 EKRKLTDEEMSTLASEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVMGDGTG- 356

Query: 122 EEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEM 181
           EEVKEEDLGKLPYLKA+VLEGLRRHPP HFVLPHAVK+DT LENYVIPKNGTVNFMVAEM
Sbjct: 357 EEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKDDTMLENYVIPKNGTVNFMVAEM 416

Query: 182 GWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILH 241
           GWDPE+WEDPMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILH
Sbjct: 417 GWDPEVWEDPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILH 476

Query: 242 LEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           LEYFIANLVWRFEWKA++GD VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 LEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPR 519

BLAST of Spg001227 vs. NCBI nr
Match: KAG7010998.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.1 bits (1250), Expect = 2.1e-133
Identity = 243/283 (85.87%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-N 61
           F E+RRNQ+KV+IPLIEARRKANQNRANR NR    E +++FV+SYVDTLLDLELPDE N
Sbjct: 237 FLEIRRNQEKVIIPLIEARRKANQNRANRENRENRAETEKDFVVSYVDTLLDLELPDEDN 296

Query: 62  RKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEE 121
           RKLTDEE+VTI SEFLNAGTDTTSTALQW+MAN+VKYPEIQ+KLF E+KGVMGD +G EE
Sbjct: 297 RKLTDEEIVTICSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLFVEMKGVMGDGTG-EE 356

Query: 122 VKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGW 181
           VKEEDLGKLPYLKA+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNGTVNFMVAEMGW
Sbjct: 357 VKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGW 416

Query: 182 DPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLE 241
           DPE+WEDPMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILHLE
Sbjct: 417 DPEVWEDPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLE 476

Query: 242 YFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           YFIANLVWRFEWKA++G+ VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 YFIANLVWRFEWKAVEGNRVDLSEKVEFTVVMEKPLKANIIPR 517

BLAST of Spg001227 vs. NCBI nr
Match: XP_023512702.1 (cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.1 bits (1250), Expect = 2.1e-133
Identity = 240/283 (84.81%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-N 61
           F E+RRNQ+KV+IPLIEARRKANQNRANR NR    E +E+FV+SYVDTLLDLELP+E N
Sbjct: 237 FLEIRRNQEKVIIPLIEARRKANQNRANRENRENRAETEEDFVVSYVDTLLDLELPEEDN 296

Query: 62  RKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEE 121
           RKLTDEE+VTI SEFLNAGTDTTSTALQW+MAN+VKYPEIQ+KL AE+KGVMGD +G EE
Sbjct: 297 RKLTDEEIVTICSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLVAEMKGVMGDGTG-EE 356

Query: 122 VKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGW 181
           VKEEDLGKLPYL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNG+VNFMVAEMGW
Sbjct: 357 VKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVNFMVAEMGW 416

Query: 182 DPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLE 241
           DPE+WEDPMAFKPERFMKGG+EEEE   FDITGSKEIKMMPFGAGRR+CPGF LAILHLE
Sbjct: 417 DPEVWEDPMAFKPERFMKGGEEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFSLAILHLE 476

Query: 242 YFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           YFIANLVWRFEWKA++GD VDLSEK+EFTVVMEKPLKA II R
Sbjct: 477 YFIANLVWRFEWKAVEGDGVDLSEKLEFTVVMEKPLKANIIPR 518

BLAST of Spg001227 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-97
Identity = 179/282 (63.48%), Postives = 225/282 (79.79%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           F ++RR Q  VL+PLI ARRK  + R     R+E +E+ +++V SYVDTLLDLELP+ENR
Sbjct: 235 FLQIRRQQRDVLLPLIRARRKIVEER----KRSE-QEDKKDYVQSYVDTLLDLELPEENR 294

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL +E+++ + SEFL AGTDTT+TALQW+MANLVKYPEIQ +L  EIK V+G+ +  +EV
Sbjct: 295 KLNEEDIMNLCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVVGEEA--KEV 354

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +EED+ K+PYLKA+VLEGLRRHPP HF+LPH+V EDT L  Y +PKNGT+NFMVAE+G D
Sbjct: 355 EEEDVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRD 414

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P  WE+PMAFKPERFM       EE A D+TGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 415 PVEWEEPMAFKPERFM------GEEEAVDLTGSRGIKMMPFGAGRRICPGIGLAMLHLEY 474

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  F+WK + G +VDL+EK+EFTVVM+ PLKA  + R
Sbjct: 475 YVANMVREFQWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

BLAST of Spg001227 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.1e-84
Identity = 156/290 (53.79%), Postives = 209/290 (72.07%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-- 61
           F ELR++Q+ V++  + AR K                E    VL YVDTLL+LE+P E  
Sbjct: 240 FLELRKSQESVILRYVNARSK----------------ETTGDVLCYVDTLLNLEIPTEEK 299

Query: 62  ----NRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVM-GD 121
                RKL+D E+V++ SEFLNA TD T+T++QW+MA +VKYPEIQ K++ E+K V  G+
Sbjct: 300 EGGKKRKLSDSEIVSLCSEFLNAATDPTATSMQWIMAIMVKYPEIQRKVYEEMKTVFAGE 359

Query: 122 RSGEEEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFM 181
               EE++EEDLGKL YLKA++LE LRRHPP H++  H V  DT L  ++IP+ GT+NFM
Sbjct: 360 EEEREEIREEDLGKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVLGGFLIPRQGTINFM 419

Query: 182 VAEMGWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGL 241
           V EMG DP++WEDP+ FKPERF++ G    E   FD+TG++EIKMMPFGAGRR+CPG+ L
Sbjct: 420 VGEMGRDPKIWEDPLTFKPERFLENG----EACDFDMTGTREIKMMPFGAGRRMCPGYAL 479

Query: 242 AILHLEYFIANLVWRFEWKALDGDDVDLSEKVEF-TVVMEKPLKAKIISR 284
           ++LHLEY++ANLVW+FEWK ++G++VDLSEK +F T+VM+ P KA I  R
Sbjct: 480 SLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFITMVMKNPFKANIYPR 509

BLAST of Spg001227 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-64
Identity = 129/278 (46.40%), Postives = 177/278 (63.67%), Query Frame = 0

Query: 4   ELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENRKL 63
           E+R+ Q + L+PLIE RR   QN  +    A           SY+DTL D+++       
Sbjct: 237 EVRKRQIETLVPLIEKRRSVVQNPGSDKTAAS---------FSYLDTLFDVKVEGRKSGP 296

Query: 64  TDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEVKE 123
           T+ E+VT+ SEFLN GTDTT+TAL+W +  L++ P IQN+L+ EIK ++GD+    +V E
Sbjct: 297 TNAELVTLCSEFLNGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTIVGDK----KVDE 356

Query: 124 EDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWDPE 183
            D+ K+PYL A+V E LR+HPP +F L H+V E   L  Y IP +  V F V  +  DP 
Sbjct: 357 NDIEKMPYLNAVVKELLRKHPPTYFTLTHSVTEPVKLAGYDIPMDTNVEFFVHGISHDPN 416

Query: 184 LWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEYFI 243
           +W DP  F P+RF+ G ++       DITG KE+KMMPFG GRRICPG G+A +H+   +
Sbjct: 417 VWSDPEKFDPDRFLSGREDA------DITGVKEVKMMPFGVGRRICPGLGMATVHVNLML 476

Query: 244 ANLVWRFEWKALDGDD-VDLSEKVEFTVVMEKPLKAKI 281
           A +V  FEW A  G++ VD SEK+EFTVVM+ PL+AK+
Sbjct: 477 ARMVQEFEWFAYPGNNKVDFSEKLEFTVVMKNPLRAKV 495

BLAST of Spg001227 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.0e-58
Identity = 125/281 (44.48%), Postives = 173/281 (61.57%), Query Frame = 0

Query: 4   ELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENRKL 63
           E+RR Q + L+P+IE RR+A QN  +         +      SY+DTL DL++  +    
Sbjct: 245 EVRREQVEFLVPIIEQRRRAIQNPGS---------DHTATTFSYLDTLFDLKVEGKKSAP 304

Query: 64  TDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEVKE 123
           +D E+V++ SEFLN GTDTT+TA++W +A L+  P +Q KL+ EIK  +    GE++V E
Sbjct: 305 SDAELVSLCSEFLNGGTDTTATAVEWGIAQLIANPNVQTKLYEEIKRTV----GEKKVDE 364

Query: 124 EDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWDPE 183
           +D+ K+PYL A+V E LR+HPP HFVL HAV E T L  Y IP +  V      +  DP+
Sbjct: 365 KDVEKMPYLHAVVKELLRKHPPTHFVLTHAVTEPTTLGGYDIPIDANVEVYTPAIAEDPK 424

Query: 184 LWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEYFI 243
            W +P  F PERF+ GG+E       DITG   +KMMPFG GRRICPG  +A +H+   +
Sbjct: 425 NWLNPEKFDPERFISGGEEA------DITGVTGVKMMPFGVGRRICPGLAMATVHIHLMM 484

Query: 244 ANLVWRFEWKALDGD-DVDLSEKVEFTVVMEKPLKAKIISR 284
           A +V  FEW A   +  +D + K EFTVVM++ L+A I  R
Sbjct: 485 ARMVQEFEWGAYPPEKKMDFTGKWEFTVVMKESLRATIKPR 506

BLAST of Spg001227 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.2e-57
Identity = 121/281 (43.06%), Postives = 171/281 (60.85%), Query Frame = 0

Query: 4   ELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENRKL 63
           E+RR Q   ++ +IE RR+A QN  +    +           SY+DTL DL++       
Sbjct: 249 EVRREQVDYVVGVIERRRRAIQNPGSDKTASS---------FSYLDTLFDLKIEGRKTTP 308

Query: 64  TDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEVKE 123
           ++EE+VT+ SEFLN GTDTT TA++W +A L+  PEIQ++L+ EIK  +GD   +  V E
Sbjct: 309 SNEELVTLCSEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTVGD---DRRVDE 368

Query: 124 EDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWDPE 183
           +D+ K+ +L+A V E LR+HPP +F L HAV E T L  Y IP    V   +  +  DP 
Sbjct: 369 KDVDKMVFLQAFVKELLRKHPPTYFSLTHAVMETTTLAGYDIPAGVNVEVYLPGISEDPR 428

Query: 184 LWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEYFI 243
           +W +P  F P+RFM  GKE+      DITG   +KM+PFG GRRICPG  +A +H+   +
Sbjct: 429 IWNNPKKFDPDRFML-GKEDA-----DITGISGVKMIPFGVGRRICPGLAMATIHVHLML 488

Query: 244 ANLVWRFEWKA-LDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           A +V  FEW A   G ++D + K+EFTVVM+ PL+A +  R
Sbjct: 489 ARMVQEFEWCAHPPGSEIDFAGKLEFTVVMKNPLRAMVKPR 511

BLAST of Spg001227 vs. ExPASy TrEMBL
Match: A0A6J1FYJ0 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448726 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.8e-134
Identity = 245/285 (85.96%), Postives = 261/285 (91.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKA---NQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPD 61
           F EL+RN+D+V IPLIEARRKA    +NR NR NRAE EEE EEFV+SYVDTLLDLE PD
Sbjct: 237 FHELKRNRDEVTIPLIEARRKAIQNRENRENRANRAEAEEEKEEFVVSYVDTLLDLEHPD 296

Query: 62  ENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGE 121
           E RKLTDEEM T+ASEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFAE+KGVMGD +G 
Sbjct: 297 EKRKLTDEEMSTLASEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVMGDGTG- 356

Query: 122 EEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEM 181
           EEVKEEDLGKLPYLKA+VLEGLRRHPP HFVLPHAVK+DT LENYVIPKNGTVNFMVAEM
Sbjct: 357 EEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKDDTMLENYVIPKNGTVNFMVAEM 416

Query: 182 GWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILH 241
           GWDPE+WEDPMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILH
Sbjct: 417 GWDPEVWEDPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILH 476

Query: 242 LEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           LEYFIANLVWRFEWKA++GD VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 LEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPR 519

BLAST of Spg001227 vs. ExPASy TrEMBL
Match: A0A6J1J7P8 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484221 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.8e-133
Identity = 243/285 (85.26%), Postives = 261/285 (91.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKA---NQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPD 61
           F EL+R +D+V IPLIEARRKA    +NRANR NRAE EEE EEFV+SYVDTLLDLE PD
Sbjct: 237 FHELKRKRDEVTIPLIEARRKAIQNRENRANRANRAEAEEEKEEFVVSYVDTLLDLEHPD 296

Query: 62  ENRKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGE 121
           E RKLTDEEM T+ASEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFA++KGVMGD +G 
Sbjct: 297 EKRKLTDEEMATLASEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFADMKGVMGDGTG- 356

Query: 122 EEVKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEM 181
           EEVKEEDLGKLPYL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNGTVNFMVAEM
Sbjct: 357 EEVKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEM 416

Query: 182 GWDPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILH 241
           GWDPE+WE+PMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILH
Sbjct: 417 GWDPEVWEEPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILH 476

Query: 242 LEYFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           LEYFIANLVWRFEWKA++GD VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 LEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPR 519

BLAST of Spg001227 vs. ExPASy TrEMBL
Match: A0A6J1FV62 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448728 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.8e-133
Identity = 242/283 (85.51%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-N 61
           F E+RRNQ+KV+IPLIEARRKANQNRANR NRAE E   E+FV+SYVDTLLDLE+PDE N
Sbjct: 237 FLEIRRNQEKVIIPLIEARRKANQNRANRENRAETE---EDFVVSYVDTLLDLEIPDEDN 296

Query: 62  RKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEE 121
           RKLTDEE+VTI SEFLNAGTD TSTALQW+MAN+VKYPEIQ+KL AE+KGVMGD +G EE
Sbjct: 297 RKLTDEEIVTICSEFLNAGTDNTSTALQWIMANIVKYPEIQHKLVAEMKGVMGDGTG-EE 356

Query: 122 VKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGW 181
           VKEEDLGKL YL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNGTVNFMVAEMGW
Sbjct: 357 VKEEDLGKLRYLRAVVLEGLRRHPPTHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGW 416

Query: 182 DPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLE 241
           DPE+WEDPMAFKPERFMKGG+EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILHLE
Sbjct: 417 DPEVWEDPMAFKPERFMKGGEEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLE 476

Query: 242 YFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           YFIANLVWRFEWKA++G+ VDLSEKVEFTVVMEKPLKA II R
Sbjct: 477 YFIANLVWRFEWKAVEGEGVDLSEKVEFTVVMEKPLKANIIPR 515

BLAST of Spg001227 vs. ExPASy TrEMBL
Match: A0A6J1FY77 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.4e-133
Identity = 244/283 (86.22%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-N 61
           F E+RRNQ+KV+IPLIEARRKANQNRANR NRAE E   E FV+SYVDTLLDLELPDE N
Sbjct: 237 FLEIRRNQEKVIIPLIEARRKANQNRANRENRAETE---EAFVVSYVDTLLDLELPDEDN 296

Query: 62  RKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEE 121
           RKLTDEE+VTI SEFLNAGTDTTSTALQW+MAN+VKYPEIQ+KLFAE+KGVMGD +G EE
Sbjct: 297 RKLTDEEIVTICSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLFAEMKGVMGDGTG-EE 356

Query: 122 VKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGW 181
           VKEEDLGKLPYL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNG+VNFMVAEMGW
Sbjct: 357 VKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVNFMVAEMGW 416

Query: 182 DPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLE 241
           DPE+WEDPMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILHLE
Sbjct: 417 DPEVWEDPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLE 476

Query: 242 YFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           YFIANLVWRFEWKA++GD VDLSEKVEFTVVM+KPLKA I  R
Sbjct: 477 YFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMDKPLKANIHPR 514

BLAST of Spg001227 vs. ExPASy TrEMBL
Match: A0A6J1JG80 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484223 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.2e-132
Identity = 242/283 (85.51%), Postives = 261/283 (92.23%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDE-N 61
           FFE+RRNQ+KV+IPLIEARRKANQNR NR +RAE E   E+FV+SYVDTLLDLELPDE N
Sbjct: 237 FFEIRRNQEKVIIPLIEARRKANQNRVNRESRAETE---EDFVVSYVDTLLDLELPDEDN 296

Query: 62  RKLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEE 121
           RKLTDEE+VTI SEFLNAGTDTTSTALQW+MANLVKYPEIQ+KLFAE+KGVMGD +G EE
Sbjct: 297 RKLTDEEIVTICSEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVMGDGTG-EE 356

Query: 122 VKEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGW 181
           VKEEDLGKLPYL+A+VLEGLRRHPP HFVLPHAVKEDT LENYVIPKNGTVNFMVAEMGW
Sbjct: 357 VKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGW 416

Query: 182 DPELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLE 241
           DPE+WE+PMAFKPERFMKGG EEEE   FDITGSKEIKMMPFGAGRR+CPGFGLAILHLE
Sbjct: 417 DPEVWEEPMAFKPERFMKGG-EEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLE 476

Query: 242 YFIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           YFIANLVWRFEWK ++GD VDLSEK EFTVVM+KPLKA I  R
Sbjct: 477 YFIANLVWRFEWKVVEGDGVDLSEKAEFTVVMDKPLKANIHPR 514

BLAST of Spg001227 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 365.9 bits (938), Expect = 2.9e-101
Identity = 180/282 (63.83%), Postives = 227/282 (80.50%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           FF++RR Q  VL+PLI ARRK  + R NR   +E EE+++E+V SYVDTLL+LELPDE R
Sbjct: 238 FFQMRREQHDVLLPLIRARRKIVEERKNR--SSEEEEDNKEYVQSYVDTLLELELPDEKR 297

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL ++E+V++ SEFLN GTDTT+TALQW+MANLVK P+IQ +L+ EIK V+G+ +   EV
Sbjct: 298 KLNEDEIVSLCSEFLNGGTDTTATALQWIMANLVKNPDIQKRLYEEIKSVVGEEA--NEV 357

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +EED  K+PYL+A+V+EGLRRHPP HFVLPH+V EDT L  Y +PKNGT+NFMVAE+G D
Sbjct: 358 EEEDAQKMPYLEAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRD 417

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P++WE+PMAFKPERFM        E A DITGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 418 PKVWEEPMAFKPERFM--------EEAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEY 477

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  F+WK + G +VDL+EK+EFTVVM+ PLKA  + R
Sbjct: 478 YVANMVREFDWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 507

BLAST of Spg001227 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 7.1e-100
Identity = 181/282 (64.18%), Postives = 224/282 (79.43%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           FF++RR Q  VL+PLI ARRK  + R NR   +E EE+++ +V SYVDTLL+LELPDE R
Sbjct: 238 FFQMRREQHDVLLPLIRARRKIVEERKNR--SSEEEEDNKVYVQSYVDTLLELELPDEKR 297

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL ++E+V++ SEFLN GTDTT+TALQW+MANLVK PEIQ +L+ EIK V+G+ +  +EV
Sbjct: 298 KLNEDEIVSLCSEFLNGGTDTTATALQWIMANLVKNPEIQKRLYEEIKSVVGEEA--KEV 357

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +EED  K+PYLKA+V+EGLRRHPP HFVLPH+V EDT L  Y +PK GT+NFMVAE+G D
Sbjct: 358 EEEDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRD 417

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P +WE+PMAFKPERFM       EE A DITGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 418 PMVWEEPMAFKPERFM------GEEEAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEY 477

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  FEWK + G +VDL+EK EFTVVM+  LKA  + R
Sbjct: 478 YVANMVREFEWKEVQGHEVDLTEKFEFTVVMKHSLKALAVLR 509

BLAST of Spg001227 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 358.6 bits (919), Expect = 4.6e-99
Identity = 176/282 (62.41%), Postives = 225/282 (79.79%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           FF+++  Q  VL+PLI ARRK    R  R   +E E++++E+V SYVDTLLD+ELPDE R
Sbjct: 239 FFQMQSEQQDVLLPLIRARRKIVDERKKR--SSEEEKDNKEYVQSYVDTLLDVELPDEKR 298

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL ++E+V++ SEFLNAGTDTT+TALQW+MANLVK PEIQ +L+ EIK ++G+ +  +EV
Sbjct: 299 KLNEDEIVSLCSEFLNAGTDTTATALQWIMANLVKNPEIQRRLYEEIKSIVGEEA--KEV 358

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +E+D  K+PYLKA+V+EGLRRHPP HFVLPH+V EDT L  Y +PK GT+NFMVAE+G D
Sbjct: 359 EEQDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRD 418

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P++WE+PMAFKPERFM        E A DITGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 419 PKVWEEPMAFKPERFM--------EEAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEY 478

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  FEW+ + G +VDL+EK+EFTVVM+ PLKA  + R
Sbjct: 479 YVANMVREFEWQEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 508

BLAST of Spg001227 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-98
Identity = 179/282 (63.48%), Postives = 225/282 (79.79%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           F ++RR Q  VL+PLI ARRK  + R     R+E +E+ +++V SYVDTLLDLELP+ENR
Sbjct: 235 FLQIRRQQRDVLLPLIRARRKIVEER----KRSE-QEDKKDYVQSYVDTLLDLELPEENR 294

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL +E+++ + SEFL AGTDTT+TALQW+MANLVKYPEIQ +L  EIK V+G+ +  +EV
Sbjct: 295 KLNEEDIMNLCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVVGEEA--KEV 354

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +EED+ K+PYLKA+VLEGLRRHPP HF+LPH+V EDT L  Y +PKNGT+NFMVAE+G D
Sbjct: 355 EEEDVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRD 414

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P  WE+PMAFKPERFM       EE A D+TGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 415 PVEWEEPMAFKPERFM------GEEEAVDLTGSRGIKMMPFGAGRRICPGIGLAMLHLEY 474

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  F+WK + G +VDL+EK+EFTVVM+ PLKA  + R
Sbjct: 475 YVANMVREFQWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

BLAST of Spg001227 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 340.9 bits (873), Expect = 9.9e-94
Identity = 171/282 (60.64%), Postives = 221/282 (78.37%), Query Frame = 0

Query: 2   FFELRRNQDKVLIPLIEARRKANQNRANRVNRAEGEEEDEEFVLSYVDTLLDLELPDENR 61
           FF++RR Q  VL+ LI ARRK  + R  R   +E EEE++E+V SYVDTLLD+ELPDE R
Sbjct: 237 FFQMRREQQDVLLRLIYARRKIVEERKKR--SSEEEEENKEYVQSYVDTLLDVELPDEKR 296

Query: 62  KLTDEEMVTIASEFLNAGTDTTSTALQWMMANLVKYPEIQNKLFAEIKGVMGDRSGEEEV 121
           KL ++E+V++ SEFL AG+DTT+T LQW+MANLVK  EIQ +L+ EI  V+G+ +  + V
Sbjct: 297 KLNEDEIVSLCSEFLIAGSDTTATVLQWIMANLVKNQEIQERLYEEITNVVGEEA--KVV 356

Query: 122 KEEDLGKLPYLKALVLEGLRRHPPAHFVLPHAVKEDTNLENYVIPKNGTVNFMVAEMGWD 181
           +E+D  K+PYLKA+V+E LRRHPP + VLPH+V EDT L  Y +PK GT+NF+VAE+G D
Sbjct: 357 EEKDTQKMPYLKAVVMEALRRHPPGNTVLPHSVTEDTVLGGYKVPKKGTINFLVAEIGRD 416

Query: 182 PELWEDPMAFKPERFMKGGKEEEEEVAFDITGSKEIKMMPFGAGRRICPGFGLAILHLEY 241
           P++WE+PMAFKPERFM       EE A DITGS+ IKMMPFGAGRRICPG GLA+LHLEY
Sbjct: 417 PKVWEEPMAFKPERFM------GEEEAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEY 476

Query: 242 FIANLVWRFEWKALDGDDVDLSEKVEFTVVMEKPLKAKIISR 284
           ++AN+V  F+WK ++G +VDL+EKVEFTV+M+ PLKA  + R
Sbjct: 477 YVANMVREFQWKEVEGHEVDLTEKVEFTVIMKHPLKAIAVPR 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010997.11.4e-13485.27Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6571189.13.2e-13484.54Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944210.11.2e-13385.96cytochrome P450 89A2-like [Cucurbita moschata][more]
KAG7010998.12.1e-13385.87Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023512702.12.1e-13384.81cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q426021.4e-9763.48Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
Q9SRQ13.1e-8453.79Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
P371231.6e-6446.40Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
O489281.0e-5844.48Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
Q9LZ317.2e-5743.06Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FYJ05.8e-13485.96cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448726 PE=3 SV=... [more]
A0A6J1J7P83.8e-13385.26cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484221 PE=3 SV=1[more]
A0A6J1FV623.8e-13385.51cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448728 PE=3 SV=... [more]
A0A6J1FY776.4e-13386.22cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=... [more]
A0A6J1JG804.2e-13285.51cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484223 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64950.12.9e-10163.83cytochrome P450, family 89, subfamily A, polypeptide 5 [more]
AT2G12190.17.1e-10064.18Cytochrome P450 superfamily protein [more]
AT1G64940.14.6e-9962.41cytochrome P450, family 87, subfamily A, polypeptide 6 [more]
AT1G64900.11.0e-9863.48cytochrome P450, family 89, subfamily A, polypeptide 2 [more]
AT1G64930.19.9e-9460.64cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 229..240
score: 37.21
coord: 78..95
score: 41.85
coord: 134..145
score: 36.19
coord: 220..229
score: 60.88
IPR001128Cytochrome P450PFAMPF00067p450coord: 6..276
e-value: 1.0E-63
score: 215.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 67..84
score: 30.19
coord: 133..151
score: 29.08
coord: 219..229
score: 58.26
coord: 174..198
score: 32.07
coord: 87..113
score: 37.01
coord: 229..252
score: 33.42
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..284
e-value: 1.5E-73
score: 250.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 3..283
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 2..281
NoneNo IPR availablePANTHERPTHR24298:SF800CYTOCHROME P450 89A2-RELATEDcoord: 2..281
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 222..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001227.1Spg001227.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen