Spg001085 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001085 vs. NCBI nr
Match: XP_022970710.1 (uncharacterized protein LOC111469611 [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 7.6e-192
Identity = 377/500 (75.40%), Postives = 413/500 (82.60%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFESAAV+L PGNLVN QIKEKLL N VSDDDKQRM+NEIARDCLQPE
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQPE 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++   N+TD D +ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHRSNQTDTDPTESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK SVKGS     ++GK       +QIKGENF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISVKGSMG---TEGK-------HQIKGENF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KRRELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRRELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M LSSS+RNKREALYLRA T+PPERPKESQKVNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. NCBI nr
Match: KAG6604805.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.2 bits (1754), Expect = 1.3e-191
Identity = 376/500 (75.20%), Postives = 414/500 (82.80%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFESAAV+L PGNLVN QIKEKLL NSVSDDDKQRM+NEIARDCLQP+
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++ N N+TD D +ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDATESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK SVKGS     ++GK       +QIKG+NF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISVKGSMG---TEGK-------HQIKGKNF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KR ELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M LSSS+RNKREALYLRA T+PPERPKESQKVNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. NCBI nr
Match: KAG7034924.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678.7 bits (1750), Expect = 3.8e-191
Identity = 374/500 (74.80%), Postives = 414/500 (82.80%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFESAAV+L PGNLVN QIKEKLL NSVSDDDKQRM+NEIARDCLQP+
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFP+ERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++ N N+TD D +ES +D
Sbjct: 241 QFPDERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDATESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK SVKGS     ++GK       +QIKG+NF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISVKGSMG---TEGK-------HQIKGKNF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KR ELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYN----GNKKDAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M LSSS+RNKREALYLRA T+PPERPKESQKVNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. NCBI nr
Match: XP_023534324.1 (uncharacterized protein LOC111795916 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.2 bits (1746), Expect = 1.1e-190
Identity = 375/500 (75.00%), Postives = 414/500 (82.80%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFESAAV+L PGNLVN QIKEKLL NSVSDDDKQRM+NEIARDCLQP+
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++ + N+TD D +ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IENSSSK SVKGS     ++GK       +QIKG+NF  +VQN        SEGATDKEM
Sbjct: 301 IENSSSKISVKGSMG---TEGK-------HQIKGKNF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KR ELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M LSSS+RNKREALYLRA T+P ERPKESQKVNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. NCBI nr
Match: XP_022947779.1 (uncharacterized protein LOC111451538 [Cucurbita moschata])

HSP 1 Score: 673.3 bits (1736), Expect = 1.6e-189
Identity = 370/500 (74.00%), Postives = 414/500 (82.80%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAF+RVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFSRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFES+AV+L PGNLVN QIKEKLL NSVSDDDKQRM+NEIARDCLQP+
Sbjct: 121 HIRKLFGERYGRRFESSAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++ N N+TD D++ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDRTESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK S+KGS     ++GK       +QIKG+NF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISIKGSMG---TEGK-------HQIKGKNF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KR ELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M L SS+RNKREALYLRA T+PPERPKESQ+VNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLPSSLRNKREALYLRAETMPPERPKESQRVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.1e-11
Identity = 36/126 (28.57%), Postives = 75/126 (59.52%), Query Frame = 0

Query: 13  KCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRMAAY 72
           K K  +K    R+++LKNKK+ I +  + +V +L+R   +++A  RVE I++DE  +  +
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  ELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGR 132
           ++++  CE +   ++ I    + P ++ E++ +L+++S R   +PEL+ I+     +YG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 RFESAA 139
             E+ A
Sbjct: 132 GLENEA 136

BLAST of Spg001085 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.1e-10
Identity = 44/167 (26.35%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           KA + +  ++ V  RLKLL+ KK+ +A++ R+++   +  G D+ A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIRKLFGE 129
            A E+L+ +C+ +L     I+  K+  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARD 176
           +Y + +         G  VN ++  KL   +      +R + EIA++
Sbjct: 127 KYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Spg001085 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.1e-10
Identity = 44/167 (26.35%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           KA + +  ++ V  RLKLL+ KK+ +A++ R+++   +  G D+ A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIRKLFGE 129
            A E+L+ +C+ +L     I+  K+  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARD 176
           +Y + +         G  VN ++  KL   +      +R + EIA++
Sbjct: 127 KYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Spg001085 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.1e-10
Identity = 44/167 (26.35%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           KA + +  ++ V  RLKLL+ KK+ +A++ R+++   +  G D+ A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIRKLFGE 129
            A E+L+ +C+ +L     I+  K+  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARD 176
           +Y + +         G  VN ++  KL   +      +R + EIA++
Sbjct: 127 KYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Spg001085 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.1e-10
Identity = 44/167 (26.35%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           KA + +  ++ V  RLKLL+ KK+ +A++ R+++   +  G D+ A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIRKLFGE 129
            A E+L+ +C+ +L     I+  K+  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARD 176
           +Y + +         G  VN ++  KL   +      +R + EIA++
Sbjct: 127 KYSKEYGKLCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Spg001085 vs. ExPASy TrEMBL
Match: A0A6J1I3N6 (uncharacterized protein LOC111469611 OS=Cucurbita maxima OX=3661 GN=LOC111469611 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.7e-192
Identity = 377/500 (75.40%), Postives = 413/500 (82.60%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFESAAV+L PGNLVN QIKEKLL N VSDDDKQRM+NEIARDCLQPE
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQPE 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++   N+TD D +ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHRSNQTDTDPTESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK SVKGS     ++GK       +QIKGENF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISVKGSMG---TEGK-------HQIKGENF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KRRELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRRELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M LSSS+RNKREALYLRA T+PPERPKESQKVNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. ExPASy TrEMBL
Match: A0A6J1G7W6 (uncharacterized protein LOC111451538 OS=Cucurbita moschata OX=3662 GN=LOC111451538 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 7.7e-190
Identity = 370/500 (74.00%), Postives = 414/500 (82.80%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MFGI FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDVVQLIRNGYDQTAF+RVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFSRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDE+R+AAYE+LDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
            IRKLFGERYGRRFES+AV+L PGNLVN QIKEKLL NSVSDDDKQRM+NEIARDCLQP+
Sbjct: 121 HIRKLFGERYGRRFESSAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 LLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLP 240
           LLAL+YRSDW++NQV+    +   DEKPDQH  Q             A+S TS+SC+SLP
Sbjct: 181 LLALQYRSDWHQNQVVARIAKA--DEKPDQHINQ-------------ANSGTSSSCDSLP 240

Query: 241 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSD 300
           QFPEERIVYLDDVVELCSST  EGDQRLFKFKTAA  PKTEI++ N N+TD D++ES +D
Sbjct: 241 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDRTESRTD 300

Query: 301 IENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEM 360
           IEN SSK S+KGS     ++GK       +QIKG+NF  +VQN        SEGATDKEM
Sbjct: 301 IENPSSKISIKGSMG---TEGK-------HQIKGKNF-TTVQN-------GSEGATDKEM 360

Query: 361 EWANFYKKP-RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           EWA+FYKKP RRR KR ELP SSDL+STTYDVFT N     NKK AA D KSN  E+ E 
Sbjct: 361 EWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYN----GNKKVAANDTKSNAMENCE- 420

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
             +M L SS+RNKREALYLRA T+PPERPKESQ+VNF RTNSCP K+P+HVHPKLPDYDD
Sbjct: 421 --EMKLPSSLRNKREALYLRAETMPPERPKESQRVNFCRTNSCPDKKPTHVHPKLPDYDD 460

Query: 481 IAAKFMALKREHLQNNTLKS 500
           IAAKFMAL+R+HLQNNTLKS
Sbjct: 481 IAAKFMALRRQHLQNNTLKS 460

BLAST of Spg001085 vs. ExPASy TrEMBL
Match: A0A6J1CEV2 (IST1-like protein isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.7e-176
Identity = 354/506 (69.96%), Postives = 398/506 (78.66%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MF  LFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDV++LI+NGY+QTAFNRVE
Sbjct: 1   MFDFLFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           QIVKDESRM AYE+L NFCEFIL NLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ
Sbjct: 61  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPE 180
           LIRKLFGERYGRRFE++AVELSPGNLVNRQIKEKL  NSVS+DDK RMINEIARDC QP+
Sbjct: 121 LIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQ 180

Query: 181 LLALEYRSDWNRNQV-LPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESL 240
           LLALEYRSDW++ QV  P    +H DE+    K++       K   +H  SA STSC++L
Sbjct: 181 LLALEYRSDWHQKQVEFPIKQAIHSDER----KREKALLKREKALLLHGYSARSTSCDTL 240

Query: 241 PQFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEIS-KANHNRTDVDQSESC 300
           PQFPEERIV++DDVVELCSSTTTEGDQ LFKFKTAA FP+ + + K + N+   D S+S 
Sbjct: 241 PQFPEERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSW 300

Query: 301 SDIENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRR-SVQNRSEGESWASEGATD 360
           S+IENSSSK S KGSK+ S++KG  KTE   N  KG N    S Q + + E+W SEGATD
Sbjct: 301 SEIENSSSKGSGKGSKRISLNKGMKKTE--HNNEKGNNCHEISEQKQRKTENWVSEGATD 360

Query: 361 KEMEWANFYKKPRRRRKRRELP---PSSDLTSTTYDVFTCNNSNMNNKKSAAKDA-KSNT 420
           KEMEWA+FYKKPRRRR+R+  P   P SDL S  YDVFT      N +    K+  KSN 
Sbjct: 361 KEMEWASFYKKPRRRRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNA 420

Query: 421 EESSEKNQKMNLSS-SVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHP 480
            ++ +     N+SS  VRNK+EALYLRAAT P   PK+S K  F RTNSCPYKQPSHVHP
Sbjct: 421 VDNGDDK---NVSSYPVRNKKEALYLRAATFP---PKQSPKACFTRTNSCPYKQPSHVHP 480

Query: 481 KLPDYDDIAAKFMALKREHLQNNTLK 499
           KLPDYDDIAAKFMALKREHLQ NT K
Sbjct: 481 KLPDYDDIAAKFMALKREHLQKNTPK 494

BLAST of Spg001085 vs. ExPASy TrEMBL
Match: A0A6J1CHT9 (IST1-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.5e-174
Identity = 354/512 (69.14%), Postives = 398/512 (77.73%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MF  LFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDV++LI+NGY+QTAFNRVE
Sbjct: 1   MFDFLFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 60

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHK------DCPNDVNEAVSSLMFASARCG 120
           QIVKDESRM AYE+L NFCEFIL NLSYIRKHK      DCPNDVNEAVSSLMFASARCG
Sbjct: 61  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCG 120

Query: 121 DLPELQLIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIAR 180
           DLPELQLIRKLFGERYGRRFE++AVELSPGNLVNRQIKEKL  NSVS+DDK RMINEIAR
Sbjct: 121 DLPELQLIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIAR 180

Query: 181 DCLQPELLALEYRSDWNRNQV-LPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATS 240
           DC QP+LLALEYRSDW++ QV  P    +H DE+    K++       K   +H  SA S
Sbjct: 181 DCFQPQLLALEYRSDWHQKQVEFPIKQAIHSDER----KREKALLKREKALLLHGYSARS 240

Query: 241 TSCESLPQFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEIS-KANHNRTDV 300
           TSC++LPQFPEERIV++DDVVELCSSTTTEGDQ LFKFKTAA FP+ + + K + N+   
Sbjct: 241 TSCDTLPQFPEERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHA 300

Query: 301 DQSESCSDIENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRR-SVQNRSEGESWA 360
           D S+S S+IENSSSK S KGSK+ S++KG  KTE   N  KG N    S Q + + E+W 
Sbjct: 301 DPSDSWSEIENSSSKGSGKGSKRISLNKGMKKTE--HNNEKGNNCHEISEQKQRKTENWV 360

Query: 361 SEGATDKEMEWANFYKKPRRRRKRRELP---PSSDLTSTTYDVFTCNNSNMNNKKSAAKD 420
           SEGATDKEMEWA+FYKKPRRRR+R+  P   P SDL S  YDVFT      N +    K+
Sbjct: 361 SEGATDKEMEWASFYKKPRRRRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKE 420

Query: 421 A-KSNTEESSEKNQKMNLSS-SVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQ 480
             KSN  ++ +     N+SS  VRNK+EALYLRAAT P   PK+S K  F RTNSCPYKQ
Sbjct: 421 TRKSNAVDNGDDK---NVSSYPVRNKKEALYLRAATFP---PKQSPKACFTRTNSCPYKQ 480

Query: 481 PSHVHPKLPDYDDIAAKFMALKREHLQNNTLK 499
           PSHVHPKLPDYDDIAAKFMALKREHLQ NT K
Sbjct: 481 PSHVHPKLPDYDDIAAKFMALKREHLQKNTPK 500

BLAST of Spg001085 vs. ExPASy TrEMBL
Match: A0A1S3BJB1 (uncharacterized protein LOC103490509 OS=Cucumis melo OX=3656 GN=LOC103490509 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.7e-173
Identity = 349/510 (68.43%), Postives = 396/510 (77.65%), Query Frame = 0

Query: 1   MFGI-LFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRV 60
           MFG   FGWRKASKCKKLIKQVQCRLKLLKNKKSVI KQLRED+VQL+ NGY QTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPEL 120
           EQIVKDE+RMAAYE+LDNFCEFILLNLSYIRKHK+CPNDVNEAVSSL+FASARCGDLPEL
Sbjct: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQP 180
           QLIRKLFGERYGR FE+ AVEL+PGNLVN QIKEKL  N VSDD+KQ+M+NEI RDCL+P
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSDWNRNQVLPSGV-QVHDDEKPDQHKKQTVNADETKRESVH-ADSATSTSCE 240
           ELLALEYRS+W++NQV    V QVH DEK +Q KK  +NA ETK+  +H +DS TSTSCE
Sbjct: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240

Query: 241 SLPQFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKAN-HNRTDVDQSE 300
             PQ PEERIVYLDDVVELCSSTTTEGDQRLFKFKT       EIS+ N HN+ D+ QSE
Sbjct: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SCSDIENSSSKTSVKGSKKR--SVSKGKPKTEDIENQIKGENFRRSVQNRSEGESW--AS 360
           S S+ ENS SKTS++GSKKR   V +GKPK ED +++      + S + R   + W  AS
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHE------KSSWKQRKMDKYWTSAS 360

Query: 361 EGATDKEMEWANFYKKP--RRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAK 420
           E  TDKE+EWANFYKKP  RRR KR + PPS D   TTYD F   N+N+N+KK+ A   K
Sbjct: 361 EITTDKEIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGF---NANINHKKAEANCRK 420

Query: 421 SNTEESSEKNQKMNLSSSVRNKREALYLRAATLPPERPKESQK-VNFPRTNSCPYKQPSH 480
            +                   K++ LYLRA T+P ERPKE  K V+F RT SCPY+QPSH
Sbjct: 421 IDV------------------KKDGLYLRAVTMPTERPKERLKEVSFGRTKSCPYEQPSH 480

Query: 481 VHPKLPDYDDIAAKFMALKREHLQNNTLKS 500
           VHPKLPDYDDIAAKF+ALKRE LQNNTLK+
Sbjct: 481 VHPKLPDYDDIAAKFIALKRERLQNNTLKT 483

BLAST of Spg001085 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.6e-54
Identity = 162/494 (32.79%), Postives = 240/494 (48.58%), Query Frame = 0

Query: 1   MFGILFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVE 60
           MF  LFGWR+ SKCK ++KQ+QCRL LLKNKK  I+  LR D+ QL+R G    A +R +
Sbjct: 71  MFCFLFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQ 130

Query: 61  QIVKDESRMAAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120
           Q+  DE+ M+ Y LL +F + ILLNLSYIR+ +D P+ +NEAVS+L+FASARCGDLPEL+
Sbjct: 131 QLFLDENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELR 190

Query: 121 LIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDC-LQP 180
            +R LFG+RYG  F   A+ L PGN VN Q+ EKL   SVSDD K +++ EI  +  L+ 
Sbjct: 191 ALRVLFGKRYGNHFVDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRL 250

Query: 181 ELLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESL 240
           E+LA+EY  ++++        QV   EK ++ K           E + ++SA        
Sbjct: 251 EVLAIEYTPEFHK--------QVLKSEKAEEEK-----------EVMSSNSAQ------- 310

Query: 241 PQFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCS 300
                            CSS   E +  ++KF          ++ A+       + + C 
Sbjct: 311 ---------------PYCSSQKAESEAEVYKF---------TLTDADIEENQEQERKVCD 370

Query: 301 DIENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKE 360
           + ++   +  V+  + +SV + +  TED   + K    RR  ++ S     +S     K+
Sbjct: 371 EEDDCIEEEVVE--EDQSVFRFRESTEDERKERK--RLRRKPRSTS-----SSSSPIAKD 430

Query: 361 MEWANFYKKPRRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEK 420
           ++   +Y K +R R+R+E           Y+VFT                          
Sbjct: 431 VDCWRYYYKGKRSRQRKEC--GKCCYHIVYNVFT-------------------------- 447

Query: 421 NQKMNLSSSVRNKREALYLRAATLPPERPKESQKVNFPRTNSCPYKQPSHVHPKLPDYDD 480
                                  LP ++  E  + +F        K+  HVHPKLPDYD 
Sbjct: 491 ----------------------MLPDQKESEEGERSF--------KKAMHVHPKLPDYDQ 447

Query: 481 IAAKFMALKREHLQ 494
           I A F AL+++  Q
Sbjct: 551 IVAHFTALRKQQQQ 447

BLAST of Spg001085 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 141.0 bits (354), Expect = 2.6e-33
Identity = 75/196 (38.27%), Postives = 117/196 (59.69%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           KA+KCK L+K    R+KL++N++    KQ+R ++ +L+  G + TA  RVE I+++E  M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGER 129
           AA E+L+ FCE I + L  I   ++CP D+ EA+SS+ FA+ RC DL ELQ ++ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 130 YGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPELLALEYRSD 189
           YG+ F +AA EL P + VNR++ E L   + S + K +++ EIA           E+  D
Sbjct: 133 YGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAE----------EHELD 192

Query: 190 WNRNQVLPSGVQVHDD 206
           W+         + H+D
Sbjct: 193 WDPASTETDLFKSHED 198

BLAST of Spg001085 vs. TAIR 10
Match: AT1G51900.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 139.8 bits (351), Expect = 5.9e-33
Identity = 92/236 (38.98%), Postives = 144/236 (61.02%), Query Frame = 0

Query: 1   MFGILFGWRKASKC--KKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNR 60
           MFG  F  R+ S     K++KQ+Q RL LLK++K   ++ LR D+V  IR+   ++A  R
Sbjct: 1   MFGFSFSRRRKSNAFTWKVLKQLQSRLMLLKSQKYAKSRHLRADIVDFIRSNDSKSALFR 60

Query: 61  VEQIVKDESRMAAYELLDNFCEFILLNLSYIRKHKD--CPNDVNEAVSSLMFASARCGDL 120
            EQ++  E+ +  Y  L  F +FILL  S  +KH      +D +EAVSSL+FAS +C ++
Sbjct: 61  TEQLLLVENAITIYGFLLKFTDFILLRFSPSKKHSCLLVNDDTSEAVSSLIFASVKCREI 120

Query: 121 PELQLIRKLFGERYGRRFESAAVELSPGNLVNRQIKEKLLTNS-VSDDDKQRMINEIARD 180
           PEL +I +L G+RYG+R+ + A+++ PGNLVN +IKEKL + S VS+ DK R++ EIA++
Sbjct: 121 PELLIISELVGQRYGQRYVTTAIQVPPGNLVNTEIKEKLKSTSVVSETDKCRVMEEIAKE 180

Query: 181 C-LQPELLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADE-TKRESVHAD 230
              + E+L L Y+S+ +          V D+   + +K    + DE +K+E+   D
Sbjct: 181 SGYRLEILGLGYKSEIDNEVFDLEEKNVMDESLHEVYKFCLTDVDERSKKETSMKD 236

HSP 2 Score: 20.4 bits (41), Expect = 5.2e+03
Identity = 94/425 (22.12%), Postives = 158/425 (37.18%), Query Frame = 0

Query: 130 YGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDC-LQPELLALEYRS 189
           Y R +E +  E+   +L +  ++E+++ N   +  K RM++EI +      ++   EY+ 
Sbjct: 366 YIRSYEESPNEVYKFSLTD--LEEEIMENDSIEGVKCRMLDEIMKKSGHHLKISRPEYKP 425

Query: 190 DWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLPQFPEERIV 249
           +  +        QV+++E     +K+ ++ D   R   + +S    S  SL  F EE  +
Sbjct: 426 EIEK--------QVYEEE-----EKKVMDPDVDIR--CYEESPHEVSKFSLTDFEEE--I 485

Query: 250 YLDDVVE-----LCSSTTTEGDQRLFKFKTAAPFPKTEISKANHN-----RTDVD---QS 309
             DD +E     +      +   RL   +     P+ EI            TD+D     
Sbjct: 486 MEDDYIEALKCRMLDDILKKSGHRLEISRRQYNKPEIEIQVNEKEEKKVINTDMDIRYDD 545

Query: 310 ESCSDIENSSSKTSVKGSKKRSVSKGKPKTEDIENQIKGENF-------RRSVQNRSEGE 369
           ES  ++E  SS T  +  +    SK     ED+ ++ +   F        RS ++ S  +
Sbjct: 546 ESPEEVETYSSLTDDEEER----SKEDTSMEDVNHKHEVYKFSLTDIKEERSNEDTSMED 605

Query: 370 SWASEGATDKEMEWANFYKKPRRRRKRRELPPS-----SDLTSTTYDVFTCNNSNMNNKK 429
               E    K+     F +    +RK    P S      D+ S TY +          + 
Sbjct: 606 CCIEEAQVGKDQRVFRFRESSEEKRKSSSSPLSPLTEFRDMESLTYYMRQKGMHRRRRRS 665

Query: 430 SAA---------KDAKSNTEESSEKNQKMNLS-------------SSVRNKREALYLRAA 489
           S +          + K   EE  E+ Q++                +  + K+E    R  
Sbjct: 666 STSPHCCHNVVYNEFKVTKEEEEEERQRLTTKRVHSKLHEYEQFLTQFKKKKEEENERRR 725

Query: 490 TLP----PERPKESQKVNFPRTNSCPYKQ-----PSHVHPKLPDYDDIAAKFMALKREHL 498
             P    P  P   Q +   R      ++       HVHPKLPDYD IA KF  LK    
Sbjct: 726 LSPKDFEPTLPDYDQVITRFRVLEKEEEERRRLATKHVHPKLPDYDQIATKFKLLKEVEK 767

BLAST of Spg001085 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-31
Identity = 83/233 (35.62%), Postives = 126/233 (54.08%), Query Frame = 0

Query: 10  KASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRM 69
           K +KCK  ++    RLK+LKNKK +  KQLR ++ QL+ +G   TA  RVE +V++E  +
Sbjct: 10  KPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTV 69

Query: 70  AAYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGER 129
           AAYEL+  +CE +++ L  I   K+CP D+ EAV+S++FAS R  D+PEL  I K F  +
Sbjct: 70  AAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTK 129

Query: 130 YGRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIAR--------------D 189
           YG+ F ++AVEL P + V+R + EKL   +     K +++  IA               D
Sbjct: 130 YGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQSFVESD 189

Query: 190 CLQPELLALEYRSDWNRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHA 229
               ELL          +  + S +  + ++ P+ H   TVNA     E  H+
Sbjct: 190 PKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHS 242

BLAST of Spg001085 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 132.5 bits (332), Expect = 9.4e-31
Identity = 126/487 (25.87%), Postives = 223/487 (45.79%), Query Frame = 0

Query: 11  ASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVVQLIRNGYDQTAFNRVEQIVKDESRMA 70
           +SKCK   K    R+KL++NK+ V+ KQ+R D+  L+++G D TA  RVE ++++++  A
Sbjct: 28  SSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQA 87

Query: 71  AYELLDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERY 130
           A E+++ FCE I+  L+ I K K CP D+ E ++SL+FA+ RC ++PEL  +R +F ++Y
Sbjct: 88  ANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKY 147

Query: 131 GRRFESAAVELSPGNLVNRQIKEKLLTNSVSDDDKQRMINEIARDCLQPELLALEYRSDW 190
           G+ F SAA +L P   VNR + +KL   +   + K +++ EIA+          E++ DW
Sbjct: 148 GKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAK----------EFQVDW 207

Query: 191 NRNQVLPSGVQVHDDEKPDQHKKQTVNADETKRESVHADSATSTSCESLPQFPEERIV-- 250
           +  +     ++  ++      K  + ++    R +++     +   +++P+      +  
Sbjct: 208 DTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPT---KAVPRSTSSMSINT 267

Query: 251 YLDDVVELCSSTTTEGDQRLFKFKTAAPFPKTEISKANHNRTDVDQSESCSDIENSSSKT 310
           +  D      + T    Q +   + A+       S         D S    D +      
Sbjct: 268 HYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHH 327

Query: 311 SVKGSKKRSVSK------GKPKTEDIENQIKGENFRRSVQNRSEGESWASEGATDKEMEW 370
              GS+++S          KP  E+     +G   R S  N    ES   E  T+ E E 
Sbjct: 328 HHPGSRRQSRDSETSSYYAKPGAEN-----RGMGRRHSYNNPGINESDYEEEYTNTEAE- 387

Query: 371 ANFYKKPRRRRKRRELPPSSDLTSTTYDVFTCNNSNMNNKKSAAKDAKSNTEESSEKNQK 430
           A    + R     R +PP +              S +   +S   + ++  +E S+    
Sbjct: 388 AKETMRRRHSYNPRSVPPPA-------------TSEIKFDESDYYEEETEPDEPSQG--- 447

Query: 431 MNLSSSVRNKREALYLRAATLPPER-PKESQKVNFPRTNSCPYKQPSHVHPKLPDYDDIA 489
                           R ++LPP R P ++ +    R +S  ++    VHPKLPDYD +A
Sbjct: 448 ----------------RVSSLPPNRAPPQAPQSGESRQDSSGHQ----VHPKLPDYDILA 459