Spg001061 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001061
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionHeavy metal-associated isoprenylated plant protein 16-like
Locationscaffold8: 40147927 .. 40149242 (-)
RNA-Seq ExpressionSpg001061
SyntenySpg001061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATCATTTAGACCAAAACTGGAGGCCGAGTTGAATTAAATTAATCGGTTAGCTCAACCAATAATCTTTATATATTTATTTATTTATTTATTGTTCTTCTTTCCCTCCACTAACCCTTACACGGTTCATCTAAAAAAAAGAAAAAGAAAAAAAGAAACTCTTACACGGTTAGATTAAATTATTTGACTTTTAATCAAAGTCTTCGTCGTTTGAAGGTTTAAAACGCGCGTTTTAGAGACATTTAAGAACTGTGCGCCCCAACGAACTTGCATTCTCTACAAAAAAAAAAAAATCGCCTATAAATATTAAATATTTTAAACCACATTTTTCTCATCACTCATAACTTTCTCTCTCTCTCTCTAAAACTTTTGTACGGCGTACGGACACTTTCTCTCTCTACAACATCAACAATAAAATGAAGGTACGTACAGATTCACCCAGGTTCTCAATCCAAAGAATTTTCAAACATCGTAAATAATCTTTTAGTTAGTTTTCCACTCGTCTTGTTCGTATGTGCTCCATTTTTTTTATATGTGTTTCACTTTGGCTTTTGCAGCAAAAGATAGTGGTTCGAGTGTCGATGAAGAGGGGCCCGAAGTACCGAAGCAAAGCGTTGAAGATTGCAGCATCCGTCTCGGGTAATCATACATACATGCATACATACGTACGTGATTGCGATTATTGTTTTGAGAGGATTTAATTTGGTTATGGTTTTGAAATTATAGGGAATGTTGAGAAGATTAGCTTGGAAGGCGACGATAAAGATAAAGTGGAGGTAATCGGAGATGTGGATGCGATCGAACTCACTGAATTGCTGCGGAAGGGTTTCGGTTCCGCACAGTTGGAGTCTGTAAGCGCCGTTGAAGATAAAGATAAAGACAAAGATAAAGACAAAGATAAAGATGATAAAGATAAACCTCAGATAACTTGGACTTATGGTTGGGGCGTGCCTCATCAACCGTCCTATTGTCATTGCTACCTTGATCCTTGCTCCTCCTATTCTTTTTGGTGATTTATATTCGATGAGGGCAAAATAGTGATTGTCAAATTCAAATTTGTAGGAAATCTGATTTAATTTTTTTTTTTGTTTTCATATTCCAATAGAAATGTGATGGATATATGCTTATATCTGTCATATATATAGATTTCCCTTTTCCTTTTTTTCCTGTTGATTTGTATTACTATCTCTCCTTTCAATATACCTCTTTTTTTTGCCATAATTTGATGCTTGAAAATTCCTAGAAAAAAGCAGTAGTAAATATTTGATATATATATTATCTCATGTACTTGTGTAAAGGTTAAAGTCTATATTGTT

mRNA sequence

ATGTGCTCCATTTTTTTTATATGTGTTTCACTTTGGCTTTTGCAGCAAAAGATAGTGGTTCGAGTGTCGATGAAGAGGGGCCCGAAGTACCGAAGCAAAGCGTTGAAGATTGCAGCATCCGTCTCGGGGAATGTTGAGAAGATTAGCTTGGAAGGCGACGATAAAGATAAAGTGGAGGTAATCGGAGATGTGGATGCGATCGAACTCACTGAATTGCTGCGGAAGGGTTTCGGTTCCGCACAGTTGGAGTCTGTAAGCGCCGTTGAAGATAAAGATAAAGACAAAGATAAAGACAAAGATAAAGATGATAAAGATAAACCTCAGATAACTTGGACTTATGGTTGGGGCGTGCCTCATCAACCGTCCTATTGTCATTGCTACCTTGATCCTTGCTCCTCCTATTCTTTTTGGTGA

Coding sequence (CDS)

ATGTGCTCCATTTTTTTTATATGTGTTTCACTTTGGCTTTTGCAGCAAAAGATAGTGGTTCGAGTGTCGATGAAGAGGGGCCCGAAGTACCGAAGCAAAGCGTTGAAGATTGCAGCATCCGTCTCGGGGAATGTTGAGAAGATTAGCTTGGAAGGCGACGATAAAGATAAAGTGGAGGTAATCGGAGATGTGGATGCGATCGAACTCACTGAATTGCTGCGGAAGGGTTTCGGTTCCGCACAGTTGGAGTCTGTAAGCGCCGTTGAAGATAAAGATAAAGACAAAGATAAAGACAAAGATAAAGATGATAAAGATAAACCTCAGATAACTTGGACTTATGGTTGGGGCGTGCCTCATCAACCGTCCTATTGTCATTGCTACCTTGATCCTTGCTCCTCCTATTCTTTTTGGTGA

Protein sequence

MCSIFFICVSLWLLQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELLRKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCYLDPCSSYSFW
Homology
BLAST of Spg001061 vs. NCBI nr
Match: XP_038900437.1 (uncharacterized protein LOC120087662 [Benincasa hispida])

HSP 1 Score: 178.7 bits (452), Expect = 3.4e-41
Identity = 92/124 (74.19%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRGPKYRSKALKIA+SV GNVE ISL GDDKDKVEV+GD+D IELT+LL
Sbjct: 1   MKQKIVIRVSMKRGPKYRSKALKIASSVKGNVETISLVGDDKDKVEVVGDLDPIELTKLL 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCYLDPCSS 133
           RK FGSAQLESV+AVE+KDKDKDKDK+        ITWT  WGVPHQ S C+CYL P  S
Sbjct: 61  RKSFGSAQLESVNAVEEKDKDKDKDKELG------ITWTCTWGVPHQSSQCYCYLGPYPS 118

Query: 134 YSFW 138
           YS W
Sbjct: 121 YSIW 118

BLAST of Spg001061 vs. NCBI nr
Match: XP_008448137.1 (PREDICTED: uncharacterized protein LOC103490424 [Cucumis melo])

HSP 1 Score: 178.7 bits (452), Expect = 3.4e-41
Identity = 93/123 (75.61%), Postives = 105/123 (85.37%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRGPKYRSKALKIAASV G++E ISL GDDKDKVEV+GD+D +ELTELL
Sbjct: 1   MKQKIVIRVSMKRGPKYRSKALKIAASVKGSIETISLVGDDKDKVEVVGDIDPMELTELL 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCY-LDPCS 133
           RKGFGSAQLE+V+AVEDKDKDKDKDK+KD      ITWT  WGVPH  SYC+CY L P  
Sbjct: 61  RKGFGSAQLETVNAVEDKDKDKDKDKNKDG----GITWTCSWGVPHHSSYCYCYNLGPYP 119

Query: 134 SYS 136
           SYS
Sbjct: 121 SYS 119

BLAST of Spg001061 vs. NCBI nr
Match: KAG7010798.1 (Heavy metal-associated isoprenylated plant protein 46, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 174.5 bits (441), Expect = 6.4e-40
Identity = 88/124 (70.97%), Postives = 105/124 (84.68%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRG KYRSKALKIAASVSG++E ISL GDDKDK+EV+GD+D IELTE+L
Sbjct: 1   MKQKIVIRVSMKRGAKYRSKALKIAASVSGDIETISLVGDDKDKIEVVGDLDPIELTEML 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCYLDPCSS 133
           RK FG AQLESVS VE K +D+D +KDKD+ ++PQITWT  WGVPHQ +YC+CY  P  S
Sbjct: 61  RKRFGCAQLESVSVVEAKKEDEDMNKDKDE-NEPQITWTCSWGVPHQTAYCYCYHCPYPS 120

Query: 134 YSFW 138
           YS W
Sbjct: 121 YSIW 123

BLAST of Spg001061 vs. NCBI nr
Match: XP_022944683.1 (uncharacterized protein LOC111449069 [Cucurbita moschata])

HSP 1 Score: 169.5 bits (428), Expect = 2.0e-38
Identity = 86/124 (69.35%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRG KYRS+ALKIAASV G++E ISL GDDKDK+EV+GD+D IELTE+L
Sbjct: 1   MKQKIVIRVSMKRGAKYRSQALKIAASVLGDIETISLVGDDKDKIEVVGDLDPIELTEML 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCYLDPCSS 133
           RK FG AQLESVS VE K +D+D +KDKD+ ++PQITWT  WGVPHQ  YC+CY  P  S
Sbjct: 61  RKRFGCAQLESVSVVEAKKEDEDVNKDKDE-NEPQITWTCSWGVPHQTPYCYCYHCPYPS 120

Query: 134 YSFW 138
           YS W
Sbjct: 121 YSIW 123

BLAST of Spg001061 vs. NCBI nr
Match: XP_004140067.1 (uncharacterized protein LOC101216311 [Cucumis sativus] >KGN46623.1 hypothetical protein Csa_005620 [Cucumis sativus])

HSP 1 Score: 168.3 bits (425), Expect = 4.6e-38
Identity = 89/125 (71.20%), Postives = 100/125 (80.00%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV++VSMKRGPKYRSKALKIAASV G++E ISL GD KDKVEV+GD+D IELTELL
Sbjct: 1   MKQKIVIQVSMKRGPKYRSKALKIAASVKGSIETISLVGDHKDKVEVVGDLDPIELTELL 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCY-LDPCS 133
           RKGFGSAQLESVSAVEDK+K KDKD          ITWT  WGVPH  SYC+CY L P  
Sbjct: 61  RKGFGSAQLESVSAVEDKEKKKDKDDG--------ITWTCSWGVPHHSSYCYCYNLRPYP 117

Query: 134 SYSFW 138
           S+S W
Sbjct: 121 SHSIW 117

BLAST of Spg001061 vs. ExPASy Swiss-Prot
Match: Q5PNZ7 (Heavy metal-associated isoprenylated plant protein 46 OS=Arabidopsis thaliana OX=3702 GN=HIPP46 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.5e-04
Identity = 43/135 (31.85%), Postives = 66/135 (48.89%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIG-DVDAIELTEL 73
           ++QKI++RV+M    K R+KA+  A    G V  + ++GD ++++EV G +VD I L ++
Sbjct: 1   MKQKILIRVTM-TDDKTRAKAMTKAVQFKG-VSAVEIKGDHRNQIEVTGVEVDMIPLIQI 60

Query: 74  LRKGFGSAQLESVSAVEDKDKDKDK-------------DKDKDDKDKPQITWTYGWGVPH 133
           LRK    A+L SV+ VE   K+ +K             D+   DK  P          P 
Sbjct: 61  LRKKVAFAELVSVTKVEPPKKEDEKKGGDGKGAEGKGGDQKGGDKKGPDDK------EPP 120

Query: 134 QPSYCHCYLDPCSSY 135
           +P    CY  P   Y
Sbjct: 121 EPKPVPCYPWPLQGY 127

BLAST of Spg001061 vs. ExPASy TrEMBL
Match: A0A1S3BIE5 (uncharacterized protein LOC103490424 OS=Cucumis melo OX=3656 GN=LOC103490424 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.6e-41
Identity = 93/123 (75.61%), Postives = 105/123 (85.37%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRGPKYRSKALKIAASV G++E ISL GDDKDKVEV+GD+D +ELTELL
Sbjct: 1   MKQKIVIRVSMKRGPKYRSKALKIAASVKGSIETISLVGDDKDKVEVVGDIDPMELTELL 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCY-LDPCS 133
           RKGFGSAQLE+V+AVEDKDKDKDKDK+KD      ITWT  WGVPH  SYC+CY L P  
Sbjct: 61  RKGFGSAQLETVNAVEDKDKDKDKDKNKDG----GITWTCSWGVPHHSSYCYCYNLGPYP 119

Query: 134 SYS 136
           SYS
Sbjct: 121 SYS 119

BLAST of Spg001061 vs. ExPASy TrEMBL
Match: A0A6J1FYA9 (uncharacterized protein LOC111449069 OS=Cucurbita moschata OX=3662 GN=LOC111449069 PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 9.9e-39
Identity = 86/124 (69.35%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRG KYRS+ALKIAASV G++E ISL GDDKDK+EV+GD+D IELTE+L
Sbjct: 1   MKQKIVIRVSMKRGAKYRSQALKIAASVLGDIETISLVGDDKDKIEVVGDLDPIELTEML 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCYLDPCSS 133
           RK FG AQLESVS VE K +D+D +KDKD+ ++PQITWT  WGVPHQ  YC+CY  P  S
Sbjct: 61  RKRFGCAQLESVSVVEAKKEDEDVNKDKDE-NEPQITWTCSWGVPHQTPYCYCYHCPYPS 120

Query: 134 YSFW 138
           YS W
Sbjct: 121 YSIW 123

BLAST of Spg001061 vs. ExPASy TrEMBL
Match: A0A0A0KAU7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G113600 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.2e-38
Identity = 89/125 (71.20%), Postives = 100/125 (80.00%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV++VSMKRGPKYRSKALKIAASV G++E ISL GD KDKVEV+GD+D IELTELL
Sbjct: 1   MKQKIVIQVSMKRGPKYRSKALKIAASVKGSIETISLVGDHKDKVEVVGDLDPIELTELL 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCY-LDPCS 133
           RKGFGSAQLESVSAVEDK+K KDKD          ITWT  WGVPH  SYC+CY L P  
Sbjct: 61  RKGFGSAQLESVSAVEDKEKKKDKDDG--------ITWTCSWGVPHHSSYCYCYNLRPYP 117

Query: 134 SYSFW 138
           S+S W
Sbjct: 121 SHSIW 117

BLAST of Spg001061 vs. ExPASy TrEMBL
Match: A0A5A7UY93 (Putative Heavy metal transport/detoxification superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G001130 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 8.4e-38
Identity = 87/113 (76.99%), Postives = 95/113 (84.07%), Query Frame = 0

Query: 24  MKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELLRKGFGSAQLE 83
           MKRGPKYRSKALKIAASV G++E ISL GDDKDKVEV+GD+D IELTELLRKGFGSAQLE
Sbjct: 1   MKRGPKYRSKALKIAASVKGSIETISLVGDDKDKVEVVGDIDPIELTELLRKGFGSAQLE 60

Query: 84  SVSAVEDKDKDKDKDKDKDDKDKPQITWTYGWGVPHQPSYCHCY-LDPCSSYS 136
           +V+AVEDKDKDKDKDK+KD      ITWT  WGVPH  SYC+CY L P  SYS
Sbjct: 61  TVNAVEDKDKDKDKDKNKDG----GITWTCSWGVPHHSSYCYCYNLGPYPSYS 109

BLAST of Spg001061 vs. ExPASy TrEMBL
Match: A0A6J1J992 (uncharacterized protein LOC111484603 OS=Cucurbita maxima OX=3661 GN=LOC111484603 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.4e-37
Identity = 85/125 (68.00%), Postives = 104/125 (83.20%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIGDVDAIELTELL 73
           ++QKIV+RVSMKRG KYRS+ALKIAASVSG++E ISL GDDKDK+EV+GD+D IEL ++L
Sbjct: 1   MKQKIVIRVSMKRGSKYRSQALKIAASVSGDIETISLVGDDKDKIEVVGDLDPIELIKML 60

Query: 74  RKGFGSAQLESVSAVEDKDKDKDKDKDKD-DKDKPQITWTYGWGVPHQPSYCHCYLDPCS 133
           RK FG AQLESVS VE K++DK+KDKDKD ++++PQITW    GVPHQ  YC+CY  P  
Sbjct: 61  RKKFGCAQLESVSVVEVKNEDKNKDKDKDKNENEPQITWMCSSGVPHQTPYCYCYHGPYP 120

Query: 134 SYSFW 138
           SYS W
Sbjct: 121 SYSIW 125

BLAST of Spg001061 vs. TAIR 10
Match: AT5G48290.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.2e-05
Identity = 43/135 (31.85%), Postives = 66/135 (48.89%), Query Frame = 0

Query: 14  LQQKIVVRVSMKRGPKYRSKALKIAASVSGNVEKISLEGDDKDKVEVIG-DVDAIELTEL 73
           ++QKI++RV+M    K R+KA+  A    G V  + ++GD ++++EV G +VD I L ++
Sbjct: 1   MKQKILIRVTM-TDDKTRAKAMTKAVQFKG-VSAVEIKGDHRNQIEVTGVEVDMIPLIQI 60

Query: 74  LRKGFGSAQLESVSAVEDKDKDKDK-------------DKDKDDKDKPQITWTYGWGVPH 133
           LRK    A+L SV+ VE   K+ +K             D+   DK  P          P 
Sbjct: 61  LRKKVAFAELVSVTKVEPPKKEDEKKGGDGKGAEGKGGDQKGGDKKGPDDK------EPP 120

Query: 134 QPSYCHCYLDPCSSY 135
           +P    CY  P   Y
Sbjct: 121 EPKPVPCYPWPLQGY 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900437.13.4e-4174.19uncharacterized protein LOC120087662 [Benincasa hispida][more]
XP_008448137.13.4e-4175.61PREDICTED: uncharacterized protein LOC103490424 [Cucumis melo][more]
KAG7010798.16.4e-4070.97Heavy metal-associated isoprenylated plant protein 46, partial [Cucurbita argyro... [more]
XP_022944683.12.0e-3869.35uncharacterized protein LOC111449069 [Cucurbita moschata][more]
XP_004140067.14.6e-3871.20uncharacterized protein LOC101216311 [Cucumis sativus] >KGN46623.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q5PNZ74.5e-0431.85Heavy metal-associated isoprenylated plant protein 46 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A1S3BIE51.6e-4175.61uncharacterized protein LOC103490424 OS=Cucumis melo OX=3656 GN=LOC103490424 PE=... [more]
A0A6J1FYA99.9e-3969.35uncharacterized protein LOC111449069 OS=Cucurbita moschata OX=3662 GN=LOC1114490... [more]
A0A0A0KAU72.2e-3871.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G113600 PE=4 SV=1[more]
A0A5A7UY938.4e-3876.99Putative Heavy metal transport/detoxification superfamily protein OS=Cucumis mel... [more]
A0A6J1J9921.4e-3768.00uncharacterized protein LOC111484603 OS=Cucurbita maxima OX=3661 GN=LOC111484603... [more]
Match NameE-valueIdentityDescription
AT5G48290.13.2e-0531.85Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 14..91
e-value: 3.6E-22
score: 80.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..108
NoneNo IPR availablePANTHERPTHR46371:SF7HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 46coord: 16..118
IPR044296Heavy metal-associated isoprenylated plant protein 16/46/47PANTHERPTHR46371OS04G0464100 PROTEINcoord: 16..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001061.1Spg001061.1mRNA