Spg000987 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000987
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Auxin-responsive GH3 family protein
Location	scaffold8: 41438826 .. 41440041 (+)
RNA-Seq Expression	Spg000987
Synteny	Spg000987

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAAAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTTCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTGTACCGTAGAGGAAGATCTCATGGAAAATGTCTCGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATAAGGGTGCTTCAATCAGTCAATATAAGACACCTCGATGCATAAATTCTGCGCAGATCATCGAGCATTTAGACTCAAACGTTGTTTCCAATTATTTTAGCCAAAAATGTCCAAAATGGGATCCCAATGAATGTCACCATCTTTTATCTTGTTAGTTGATGATGTAAGTTAATTCAAGATAAGTCCTCATGTATTTTCATGTTTGTGGTTCGTTTGCACTATTTAGGGTGGTCGATCAATTTGCCTTCTGTGGAGAGATTATCTTGGTTTTTTGGTTTGGTTGGCTTGGTCACTCATAACCTTTGGCTTTGAGTGACATTCTTTGAGCCACATAATTTAAAAATAAAACTCCAATATTTAGTTTTCTATGGAAGAAAAATATATAGTTATAAGCCTTAATTTGAGAAAATAGATAAATCGAAACGTCATAATGTTGTACAAAAAGTTACTCATCTAAGCCAAGTTACTCATCTAAGACACCGAGACTATGTGTAATGACCCGACCAAAAAGGGATTAAAATCACGTGAACGTAAAACTTTGAAAACTTTAAAGCGGAAGAGATCATAAGTACACATCTTTTGTTTAATAAACCGGGCCCAAAACCATAAGTCCATTAACAATATCATAATAGTCAAAACATGATAGACAACTTTCGTAAAATTGAACAATTAAAACATTTTAAAACATGAGACTAAGAAACTCAATCCTAGACTTCTATGGACATGATTTGCAGCTGCATCCCATAGCCTAGCCAAGTCACTGCCATCGCCACTCGCCTGATGTCACCAAGTACCACTTGA

mRNA sequence

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAAAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTTCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTGTACCGTAGAGGAAGATCTCATGGAAAATGTCTCGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATAAGGGTGCTTCAATCAGTCAATATAAGACACCTCGATGCATAAATTCTGCGCAGATCATCGAGCATTTAGACTCAAACGTTCCTAGCCAAGTCACTGCCATCGCCACTCGCCTGATGTCACCAAGTACCACTTGA

Coding sequence (CDS)

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAAAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTTCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTGTACCGTAGAGGAAGATCTCATGGAAAATGTCTCGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATAAGGGTGCTTCAATCAGTCAATATAAGACACCTCGATGCATAAATTCTGCGCAGATCATCGAGCATTTAGACTCAAACGTTCCTAGCCAAGTCACTGCCATCGCCACTCGCCTGATGTCACCAAGTACCACTTGA

Protein sequence

MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDYTSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPLEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPSQVTAIATRLMSPSTT

Homology

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BLAST of Spg000987 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 258.5 bits (659), Expect = 4.5e-65
Identity = 126/166 (75.90%), Postives = 142/166 (85.54%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           MGDILRVTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQ+AA+LL PFGA I DY
Sbjct: 399 MGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  I IPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIKIV+VGTFEKLM +A+N+GAS+SQYKTPRC+NS  IIE LDSNV
Sbjct: 519 EIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNV 564

[Close]

BLAST of Spg000987 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 255.8 bits (652), Expect = 2.9e-64
Identity = 125/166 (75.30%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           MGDIL VTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQ+AA+LL PFGA I DY
Sbjct: 399 MGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  I IPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIKIV+VGTFEKLM +A+N+GAS+SQYKTPRC+NS  IIE LDSNV
Sbjct: 519 EIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNV 564

[Close]

BLAST of Spg000987 vs. NCBI nr
Match: XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 250.0 bits (637), Expect = 1.6e-62
Identity = 122/167 (73.05%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDI+RVTGFK KAP FSFVCRKN VLSLD EKTDEAELHNAV+ A +LL PFGATI DY
Sbjct: 346 IGDIVRVTGFKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADY 405

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYAD++T PGHYVLYWEI  + EI  IPSSIYEDCCL VEESL+ LYR+ RSH K + P
Sbjct: 406 TSYADNSTFPGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINP 465

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           LEIK+VE GTF+KL++RA  +GASISQYKTPRC+ S QI+E LD+ V
Sbjct: 466 LEIKMVEAGTFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKV 512

[Close]

BLAST of Spg000987 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 249.6 bits (636), Expect = 2.1e-62
Identity = 123/167 (73.65%), Postives = 137/167 (82.04%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDI+RVTGFKNKAP F FV RKN +LSLDVEKTDEAELHNAV K   LL  FGA +VDY
Sbjct: 399 IGDIVRVTGFKNKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  + EI  IPSSIYEDCCL +EESLS +YRR RSH K + P
Sbjct: 459 TSYADSSTFPGHYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINP 518

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           LEIKIVE  TF+KLM+R V++GASISQYKTPRC+ S QIIE LDS V
Sbjct: 519 LEIKIVEARTFDKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKV 565

[Close]

BLAST of Spg000987 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 247.7 bits (631), Expect = 7.9e-62
Identity = 121/169 (71.60%), Postives = 140/169 (82.84%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           MGDILRVTGFKNK P FSF CRKN +LSLD+EKTDE +LH+AV +A+ LL P GA IVDY
Sbjct: 401 MGDILRVTGFKNKTPNFSFACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDY 460

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  R E   IPS++YEDCCL VEESLS +YR+ RS+ K + P
Sbjct: 461 TSYADSSTFPGHYVLYWEIQCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINP 520

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           LEIKIVE GTFEKLM++AV++GASISQYKTPRC+ S Q+IE LDS V S
Sbjct: 521 LEIKIVETGTFEKLMEQAVDRGASISQYKTPRCLKSTQMIELLDSKVVS 569

[Close]

BLAST of Spg000987 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.2e-56
Identity = 104/166 (62.65%), Postives = 131/166 (78.92%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+LRVTGFKNKAP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL VEES + +YR+GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIKIVE GTF+KLM  A++ GASI+QYKTPRC+  A IIE L+S V
Sbjct: 545 EIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRV 590

[Close]

BLAST of Spg000987 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.2e-54
Identity = 100/166 (60.24%), Postives = 129/166 (77.71%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+L V GFKN AP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL +EESL+ +YR+GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIK+VE GTF+KLM  A++ GASI+QYKTPRC+  A IIE L+S V
Sbjct: 545 EIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRV 590

[Close]

BLAST of Spg000987 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.1e-53
Identity = 96/166 (57.83%), Postives = 129/166 (77.71%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+LRV GFKNKAP FSFV R+N  LS+D +KTDE ELH AV  A Q L PFGA++V+Y
Sbjct: 415 VGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEY 474

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+ TIPGHYVL+WE L      +P+S++E+CCL VEE+L+ +YR+GR+  + +GPL
Sbjct: 475 TSYADAATIPGHYVLFWE-LRAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPL 534

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EI++V  GTF+KLM  A+++GASI+QYK PRC+    ++E LD+ V
Sbjct: 535 EIRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARV 579

[Close]

BLAST of Spg000987 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-50
Identity = 96/168 (57.14%), Postives = 128/168 (76.19%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+LRV GFKN AP F+FV RKN  LS+D +KTDEAELH AV +A Q L PFGA++V+Y
Sbjct: 423 VGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEY 482

Query: 61  TSYAD-STTIPGHYVLYWEILHRI-EIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLG 120
           TSYAD +TTIPGHYVL+WE+        +P+S++EDCCL VEE L+ +YR+ R+  + +G
Sbjct: 483 TSYADTATTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIG 542

Query: 121 PLEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           PLEI++V  GTF+KLM  A+++GASI+QYK PRC+    ++E LD  V
Sbjct: 543 PLEIRVVADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRV 590

[Close]

BLAST of Spg000987 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.0e-49
Identity = 99/187 (52.94%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDIL+VTGF N AP+F FV RKN +LS+D +KTDEAEL  AV++A+ LL P+GA+IV+Y
Sbjct: 426 VGDILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEY 485

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIP-------SSIYEDCCLVVEESLSILYRRGRSH 120
           TS AD+TTIPGHYV+YWE++ R     P         ++E CCL +EE+L+ +YR+GR +
Sbjct: 486 TSQADATTIPGHYVVYWELMVREGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGR-N 545

Query: 121 GKCLGPLEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPSQVTAI 180
           G+ +GPLEI++V  GTFE++M  A+++GASI+QYK PRC++   IIE L+S V S+  + 
Sbjct: 546 GEAIGPLEIRVVRAGTFEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSP 605

[Close]

BLAST of Spg000987 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.4e-64
Identity = 125/166 (75.30%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           MGDIL VTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQ+AA+LL PFGA I DY
Sbjct: 399 MGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  I IPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIKIV+VGTFEKLM +A+N+GAS+SQYKTPRC+NS  IIE LDSNV
Sbjct: 519 EIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNV 564

[Close]

BLAST of Spg000987 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.7e-63
Identity = 122/167 (73.05%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDI+RVTGFK KAP FSFVCRKN VLSLD EKTDEAELHNAV+ A +LL PFGATI DY
Sbjct: 346 IGDIVRVTGFKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADY 405

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYAD++T PGHYVLYWEI  + EI  IPSSIYEDCCL VEESL+ LYR+ RSH K + P
Sbjct: 406 TSYADNSTFPGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINP 465

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           LEIK+VE GTF+KL++RA  +GASISQYKTPRC+ S QI+E LD+ V
Sbjct: 466 LEIKMVEAGTFDKLLERATYQGASISQYKTPRCVTSTQIVELLDTKV 512

[Close]

BLAST of Spg000987 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.0e-62
Identity = 123/167 (73.65%), Postives = 137/167 (82.04%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDI+RVTGFKNKAP F FV RKN +LSLDVEKTDEAELHNAV K   LL  FGA +VDY
Sbjct: 399 IGDIVRVTGFKNKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  + EI  IPSSIYEDCCL +EESLS +YRR RSH K + P
Sbjct: 459 TSYADSSTFPGHYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINP 518

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           LEIKIVE  TF+KLM+R V++GASISQYKTPRC+ S QIIE LDS V
Sbjct: 519 LEIKIVEARTFDKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKV 565

[Close]

BLAST of Spg000987 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.8e-62
Identity = 121/169 (71.60%), Postives = 140/169 (82.84%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           MGDILRVTGFKNK P FSF CRKN +LSLD+EKTDE +LH+AV +A+ LL P GA IVDY
Sbjct: 401 MGDILRVTGFKNKTPNFSFACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDY 460

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  R E   IPS++YEDCCL VEESLS +YR+ RS+ K + P
Sbjct: 461 TSYADSSTFPGHYVLYWEIQCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINP 520

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           LEIKIVE GTFEKLM++AV++GASISQYKTPRC+ S Q+IE LDS V S
Sbjct: 521 LEIKIVETGTFEKLMEQAVDRGASISQYKTPRCLKSTQMIELLDSKVVS 569

[Close]

BLAST of Spg000987 vs. ExPASy TrEMBL
Match: A0A2I0HPT0 (indole-3-acetic acid-amido synthetase GH3.6 OS=Punica granatum OX=22663 GN=LOC116193173 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.4e-56
Identity = 105/168 (62.50%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+LRV GFKNKAP+F+F+CRKN VLS+D +KTDE EL NAV+ A   L PFGAT+ +Y
Sbjct: 427 VGDVLRVAGFKNKAPQFNFICRKNVVLSIDADKTDEVELQNAVRNALNHLTPFGATVTEY 486

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+TTIPGHYVL+WE+       IP S+YEDCCL +EESL+ +YR+GR+  K +GPL
Sbjct: 487 TSYADTTTIPGHYVLFWELALNGSTAIPPSVYEDCCLAIEESLNSVYRQGRASDKSIGPL 546

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           EI+IVE GTF+KLM  A++ GASI+QYKTPRC+  A ++E L+S V S
Sbjct: 547 EIRIVESGTFDKLMDCAISYGASINQYKTPRCVKFAPLLEVLNSRVTS 594

[Close]

BLAST of Spg000987 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 218.8 bits (556), Expect = 3.7e-57
Identity = 104/166 (62.65%), Postives = 131/166 (78.92%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+LRVTGFKNKAP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL VEES + +YR+GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIKIVE GTF+KLM  A++ GASI+QYKTPRC+  A IIE L+S V
Sbjct: 545 EIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRV 590

[Close]

BLAST of Spg000987 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-55
Identity = 100/166 (60.24%), Postives = 129/166 (77.71%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GD+L V GFKN AP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL +EESL+ +YR+GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNV 167
           EIK+VE GTF+KLM  A++ GASI+QYKTPRC+  A IIE L+S V
Sbjct: 545 EIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRV 590

[Close]

BLAST of Spg000987 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.4e-48
Identity = 91/169 (53.85%), Postives = 128/169 (75.74%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDIL+VTGF N AP+F FV RKN +LS++ +KTDEAEL +AV+ A+ LLG  G  +++Y
Sbjct: 411 VGDILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEY 470

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIFIPS-SIYEDCCLVVEESLSILYRRGRSHGKCLGP 120
           TSYA++ TIPGHYV+YWE+L + +   P+  +   CCL +EESL+ +YR+ R   K +GP
Sbjct: 471 TSYAETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGP 530

Query: 121 LEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           LEI++V+ GTFE+LM  A+++GASI+QYK PRC++   I+E LDS V S
Sbjct: 531 LEIRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVS 579

[Close]

BLAST of Spg000987 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 188.0 bits (476), Expect = 6.9e-48
Identity = 89/170 (52.35%), Postives = 124/170 (72.94%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDILRVTGF N AP+F F+ RKN +LS++ +KTDEAEL  AV+ A++L    G  +++Y
Sbjct: 418 VGDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEY 477

Query: 61  TSYADSTTIPGHYVLYWEILHRIE--IFIPSSIYEDCCLVVEESLSILYRRGRSHGKCLG 120
           TSYA++ TIPGHYV+YWE+L R +    +   +   CCL +EESL+ +YR+ R   K +G
Sbjct: 478 TSYAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIG 537

Query: 121 PLEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           PLEI++V  GTFE+LM  A+++GASI+QYK PRC++   I+E LDS V S
Sbjct: 538 PLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVS 587

[Close]

BLAST of Spg000987 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 186.0 bits (471), Expect = 2.6e-47
Identity = 88/170 (51.76%), Postives = 128/170 (75.29%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQKAAQLLGPFGATIVDY 60
           +GDILRVTGF N AP+F F+ R+N +LS++ +KTDEA+L  AV+ A++LL   G  +++Y
Sbjct: 412 VGDILRVTGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEY 471

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-FIPS-SIYEDCCLVVEESLSILYRRGRSHGKCLG 120
           TSYAD+ TIPGHYV+YWE+L R +   +PS  +   CCL +EESL+ +YR+ R   K +G
Sbjct: 472 TSYADTKTIPGHYVIYWELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIG 531

Query: 121 PLEIKIVEVGTFEKLMKRAVNKGASISQYKTPRCINSAQIIEHLDSNVPS 169
           PLEI++V+ GTFE+LM  ++++G+SI+QYK PRC++   I++ LDS V S
Sbjct: 532 PLEIRVVQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVS 581

The following BLAST results are available for this feature:

BLAST of Spg000987 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570863.1	4.5e-65	75.90	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	2.9e-64	75.30	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_022140614.1	1.6e-62	73.05	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_022140778.1	2.1e-62	73.65	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_022140368.1	7.9e-62	71.60	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]

BLAST of Spg000987 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	5.2e-56	62.65	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	2.2e-54	60.24	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	9.1e-53	57.83	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	1.5e-50	57.14	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
P0C0M2	8.0e-49	52.94	Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo...	[more]

BLAST of Spg000987 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUU5	1.4e-64	75.30	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CG61	7.7e-63	73.05	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CIS9	1.0e-62	73.65	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CHU9	3.8e-62	71.60	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A2I0HPT0	2.4e-56	62.50	indole-3-acetic acid-amido synthetase GH3.6 OS=Punica granatum OX=22663 GN=LOC11...	[more]

BLAST of Spg000987 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	3.7e-57	62.65	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.5e-55	60.24	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	2.4e-48	53.85	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	6.9e-48	52.35	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	2.6e-47	51.76	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 1..156 e-value: 2.7E-50 score: 171.8
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 1..169
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 1..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000987.1	Spg000987.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity