Spg000948 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000948
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pyruvate kinase family protein
Location	scaffold8: 48221638 .. 48222195 (+)
RNA-Seq Expression	Spg000948
Synteny	Spg000948

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACTACAACATCTCTGTACTAGATGCAATTTCAGTCCTGTTATCTTTCTGGAATTTAACTCTCACTCTCAGGTTGAGAATGAAGAAGGGAATGGAAATTCCAATTGAGAAGATATTTCTGGCTCAGAAAGTGATGATATACAAATGCAACATCCAGGGAAAACCTATTGTCACAGCGACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCGGAAGCCACTGATGTTGCAAATGCTGTTTTTGATGGGGCAGATTGCGTAATGTTAAGTGGTGAAACGGCTGCTGGAGCATATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACTCTTGATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCAGTTCCCATGAGCCGATTAGAAAGCTTGGCTTCTTCTGCTGTTAGAACTGCAAACTCGGCCAAGGCAACTCTTATACTAGTCCTAACCATCTTGTCTGTGGTTGTCCCCGAGATTCAGACAAATTCATTTGATTGGTCGTGCAGTGATCAGTGA

mRNA sequence

ATGTACTACAACATCTCTGTACTAGATGCAATTTCAGTCCTGTTATCTTTCTGGAATTTAACTCTCACTCTCAGGTTGAGAATGAAGAAGGGAATGGAAATTCCAATTGAGAAGATATTTCTGGCTCAGAAAGTGATGATATACAAATGCAACATCCAGGGAAAACCTATTGTCACAGCGACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCGGAAGCCACTGATGTTGCAAATGCTGTTTTTGATGGGGCAGATTGCGTAATGTTAAGTGGTGAAACGGCTGCTGGAGCATATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACTCTTGATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCAGTTCCCATGAGCCGATTAGAAAGCTTGGCTTCTTCTGCTGTTAGAACTGCAAACTCGGCCAAGGCAACTCTTATACTAGTCCTAACCATCTTGTCTGTGGTTGTCCCCGAGATTCAGACAAATTCATTTGATTGGTCGTGCAGTGATCAGTGA

Coding sequence (CDS)

ATGTACTACAACATCTCTGTACTAGATGCAATTTCAGTCCTGTTATCTTTCTGGAATTTAACTCTCACTCTCAGGTTGAGAATGAAGAAGGGAATGGAAATTCCAATTGAGAAGATATTTCTGGCTCAGAAAGTGATGATATACAAATGCAACATCCAGGGAAAACCTATTGTCACAGCGACACAGATGCTGGAATCAATGATTAAATCTCCAAGACCAACTAGAGCGGAAGCCACTGATGTTGCAAATGCTGTTTTTGATGGGGCAGATTGCGTAATGTTAAGTGGTGAAACGGCTGCTGGAGCATATCCAGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACTCTTGATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCAGTTCCCATGAGCCGATTAGAAAGCTTGGCTTCTTCTGCTGTTAGAACTGCAAACTCGGCCAAGGCAACTCTTATACTAGTCCTAACCATCTTGTCTGTGGTTGTCCCCGAGATTCAGACAAATTCATTTGATTGGTCGTGCAGTGATCAGTGA

Protein sequence

MYYNISVLDAISVLLSFWNLTLTLRLRMKKGMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGADCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSVPMSRLESLASSAVRTANSAKATLILVLTILSVVVPEIQTNSFDWSCSDQ

Homology

[Close]

BLAST of Spg000948 vs. NCBI nr
Match: XP_021692461.1 (pyruvate kinase, cytosolic isozyme-like isoform X1 [Hevea brasiliensis] >XP_021692462.1 pyruvate kinase, cytosolic isozyme-like isoform X2 [Hevea brasiliensis])

HSP 1 Score: 269.2 bits (687), Expect = 2.6e-68
Identity = 146/173 (84.39%), Postives = 153/173 (88.44%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG+D
Sbjct: 263 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGSD 322

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKR+MEHS VPMS LESLASSAV
Sbjct: 323 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRVMEHSPVPMSPLESLASSAV 382

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA+LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 383 RTANSAKASLILVLTRGGSTAKLVAKYRPGLPILSVVVPEIETDSFDWSCSDE 435

[Close]

BLAST of Spg000948 vs. NCBI nr
Match: XP_038901386.1 (pyruvate kinase, cytosolic isozyme [Benincasa hispida])

HSP 1 Score: 268.9 bits (686), Expect = 3.4e-68
Identity = 147/173 (84.97%), Postives = 152/173 (87.86%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA+LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKASLILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. NCBI nr
Match: XP_023512551.1 (pyruvate kinase, cytosolic isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.5 bits (685), Expect = 4.4e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. NCBI nr
Match: XP_022944286.1 (pyruvate kinase, cytosolic isozyme-like [Cucurbita moschata] >KAG7010207.1 hypothetical protein SDJN02_26999 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 268.5 bits (685), Expect = 4.4e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. NCBI nr
Match: XP_022153828.1 (pyruvate kinase, cytosolic isozyme [Momordica charantia])

HSP 1 Score: 268.5 bits (685), Expect = 4.4e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 1.9e-66
Identity = 138/173 (79.77%), Postives = 148/173 (85.55%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 266 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 325

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYP+LAV TMAKIC+EAEST+DY DVFKRIM ++ VPMS LESLASSAV
Sbjct: 326 CVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSNAPVPMSPLESLASSAV 385

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDW+CSD+
Sbjct: 386 RTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTDSFDWTCSDE 438

[Close]

BLAST of Spg000948 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-65
Identity = 134/173 (77.46%), Postives = 147/173 (84.97%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCN  GKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 255 GMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 314

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEH-SVPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGD+FKRIM H +VPMS +ESLASSAV
Sbjct: 315 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPMESLASSAV 374

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTA S++ATL++VLT                 ILSVVVPEI ++SFDW+CS++
Sbjct: 375 RTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSNE 427

[Close]

BLAST of Spg000948 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-60
Identity = 123/173 (71.10%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIP+EKIFLAQK+MIYKCN+ GKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 269 GMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 328

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGE+AAGAYPELAV+ MA+IC+EAES+LDYG +FK ++  + +PMS LESLASSAV
Sbjct: 329 CVMLSGESAAGAYPELAVKIMARICIEAESSLDYGAIFKEMIRSTPLPMSPLESLASSAV 388

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTAN AKA LI+VLT                 ILSVVVP + T+SFDW+CSD+
Sbjct: 389 RTANKAKAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLSTDSFDWTCSDE 441

[Close]

BLAST of Spg000948 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.7e-54
Identity = 117/173 (67.63%), Postives = 137/173 (79.19%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIP+EKIFLAQK+MIYKCN+ GK +VTATQMLESMIKSP PTRAEATDVANAV DG D
Sbjct: 268 GMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQMLESMIKSPAPTRAEATDVANAVLDGTD 327

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGE+AAGAYPELAV+ M++IC+EAES+LD   +FK ++  + +PMS LESLASSAV
Sbjct: 328 CVMLSGESAAGAYPELAVKIMSRICIEAESSLDNEAIFKEMIRCTPLPMSPLESLASSAV 387

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTAN A+A LI+VLT                 ILSVVVP + T+SFDWS SD+
Sbjct: 388 RTANKARAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLTTDSFDWSISDE 440

[Close]

BLAST of Spg000948 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.6e-39
Identity = 83/135 (61.48%), Postives = 104/135 (77.04%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIP EK+FLAQK+MI KCN+ GKP++TATQMLESM K+PRPTRAEA DVANAV DG D
Sbjct: 303 GMEIPPEKVFLAQKMMISKCNVAGKPVITATQMLESMTKNPRPTRAEAADVANAVLDGTD 362

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSV-PMSRLESLASSAV 150
           CVMLSGETA G++P  AV  M+++C EAE  +DY  VF+   + ++ P+   E++A +AV
Sbjct: 363 CVMLSGETANGSFPVQAVTVMSRVCFEAEGCIDYQQVFRATCQATMTPIDTQEAVARAAV 422

Query: 151 RTANSAKATLILVLT 165
            TA S  A+LIL LT
Sbjct: 423 ETAQSINASLILALT 437

[Close]

BLAST of Spg000948 vs. ExPASy TrEMBL
Match: A0A1L1Y9P7 (Pyruvate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.1e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. ExPASy TrEMBL
Match: A0A0A0L1N0 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.1e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. ExPASy TrEMBL
Match: A0A6J1DIL1 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111021251 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.1e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. ExPASy TrEMBL
Match: A0A6J1FU03 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111448777 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.1e-68
Identity = 147/173 (84.97%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 258 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 317

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 318 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 377

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 378 RTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDE 430

[Close]

BLAST of Spg000948 vs. ExPASy TrEMBL
Match: A0A7J9BK31 (Pyruvate kinase OS=Gossypium gossypioides OX=34282 GN=Gogos_010098 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.8e-68
Identity = 146/173 (84.39%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVM+YKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 143 GMEIPIEKIFLAQKVMVYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 202

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS VPMS LESLASSAV
Sbjct: 203 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHSPVPMSPLESLASSAV 262

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSAKA LILVLT                 ILSVVVPEI+T+SFDWSCSD+
Sbjct: 263 RTANSAKAALILVLTRGGSTAKLVAKYRPGIPILSVVVPEIKTDSFDWSCSDE 315

[Close]

BLAST of Spg000948 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 260.8 bits (665), Expect = 8.5e-70
Identity = 143/173 (82.66%), Postives = 150/173 (86.71%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCNIQGKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 256 GMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 315

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIM +S VPMS LESLASSAV
Sbjct: 316 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLYSPVPMSPLESLASSAV 375

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTANSA+ATLI+VLT                 ILSVVVPEI+T+ FDWSCSD+
Sbjct: 376 RTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFDWSCSDE 428

[Close]

BLAST of Spg000948 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-66
Identity = 134/173 (77.46%), Postives = 147/173 (84.97%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQKVMIYKCN  GKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 255 GMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 314

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEH-SVPMSRLESLASSAV 150
           CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGD+FKRIM H +VPMS +ESLASSAV
Sbjct: 315 CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPMESLASSAV 374

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTA S++ATL++VLT                 ILSVVVPEI ++SFDW+CS++
Sbjct: 375 RTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSNE 427

[Close]

BLAST of Spg000948 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 230.3 bits (586), Expect = 1.2e-60
Identity = 123/173 (71.10%), Postives = 141/173 (81.50%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQK+MIYKCN+ GKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 268 GMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 327

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGE+AAGAYPE+AV+ MAKIC+EAES+LDY  +FK ++  + +PMS LESLASSAV
Sbjct: 328 CVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIRATPLPMSPLESLASSAV 387

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTAN A+A LI+VLT                 ILSVVVP + T+SFDWSCSD+
Sbjct: 388 RTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSDE 440

[Close]

BLAST of Spg000948 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 228.8 bits (582), Expect = 3.6e-60
Identity = 122/173 (70.52%), Postives = 141/173 (81.50%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEKIFLAQK+MIYKCN+ GKP+VTATQMLESMIKSPRPTRAEATDVANAV DG D
Sbjct: 268 GMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTD 327

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEHS-VPMSRLESLASSAV 150
           CVMLSGE+AAGAYPE+AV+TMAKIC+EAES+LDY  +FK ++  + +PMS LESLASSAV
Sbjct: 328 CVMLSGESAAGAYPEIAVKTMAKICIEAESSLDYNTIFKEMIRATPLPMSTLESLASSAV 387

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEIQTNSFDWSCSDQ 186
           RTAN AKA LI+VLT                 ILSVVVP   +++F+WSCSD+
Sbjct: 388 RTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSCSDE 440

[Close]

BLAST of Spg000948 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 191.8 bits (486), Expect = 4.9e-49
Identity = 110/173 (63.58%), Postives = 129/173 (74.57%), Query Frame = 0


Query: 31  GMEIPIEKIFLAQKVMIYKCNIQGKPIVTATQMLESMIKSPRPTRAEATDVANAVFDGAD 90
           GMEIPIEK+FLAQK+MI K N  GKPIVTATQMLESM KSPRPTRAEATDVANAV DG D
Sbjct: 264 GMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPTRAEATDVANAVLDGTD 323

Query: 91  CVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIME-HSVPMSRLESLASSAV 150
           CVMLSGETAAGA+PE AV TM++IC EAE  +DY  + K+I +  S+P+S +ESLA+SAV
Sbjct: 324 CVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHKKIQDIVSLPLSPIESLAASAV 383

Query: 151 RTANSAKATLILVLT-----------------ILSVVVPEI-QTNSFDWSCSD 185
            TA S  A  I+VLT                 ILSV+VPEI +T+ F+WSCS+
Sbjct: 384 STARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRTDDFEWSCSE 436

The following BLAST results are available for this feature:

BLAST of Spg000948 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_021692461.1	2.6e-68	84.39	pyruvate kinase, cytosolic isozyme-like isoform X1 [Hevea brasiliensis] >XP_0216...	[more]
XP_038901386.1	3.4e-68	84.97	pyruvate kinase, cytosolic isozyme [Benincasa hispida]	[more]
XP_023512551.1	4.4e-68	84.97	pyruvate kinase, cytosolic isozyme-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944286.1	4.4e-68	84.97	pyruvate kinase, cytosolic isozyme-like [Cucurbita moschata] >KAG7010207.1 hypot...	[more]
XP_022153828.1	4.4e-68	84.97	pyruvate kinase, cytosolic isozyme [Momordica charantia]	[more]

BLAST of Spg000948 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42954	1.9e-66	79.77	Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O65595	2.8e-65	77.46	Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q42806	7.8e-60	71.10	Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1	[more]
P22200	1.7e-54	67.63	Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
O44006	7.6e-39	61.48	Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1	[more]

BLAST of Spg000948 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1L1Y9P7	2.1e-68	84.97	Pyruvate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A0A0L1N0	2.1e-68	84.97	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1	[more]
A0A6J1DIL1	2.1e-68	84.97	Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111021251 PE=3 SV=1	[more]
A0A6J1FU03	2.1e-68	84.97	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111448777 PE=3 SV=1	[more]
A0A7J9BK31	2.8e-68	84.39	Pyruvate kinase OS=Gossypium gossypioides OX=34282 GN=Gogos_010098 PE=3 SV=1	[more]

BLAST of Spg000948 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56350.1	8.5e-70	82.66	Pyruvate kinase family protein	[more]
AT4G26390.1	2.0e-66	77.46	Pyruvate kinase family protein	[more]
AT5G08570.1	1.2e-60	71.10	Pyruvate kinase family protein	[more]
AT5G63680.1	3.6e-60	70.52	Pyruvate kinase family protein	[more]
AT3G04050.1	4.9e-49	63.58	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 77..95 score: 75.3 coord: 27..51 score: 51.69 coord: 96..112 score: 58.37 coord: 52..76 score: 68.62
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 27..182
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 30..126 e-value: 2.6E-48 score: 164.7
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 29..118 e-value: 3.9E-60 score: 205.7
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 119..164 e-value: 3.9E-60 score: 205.7
None	No IPR available	PANTHER	PTHR11817:SF90	PYRUVATE KINASE	coord: 27..182
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 31..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000948.1	Spg000948.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0003824	catalytic activity