Spg000866 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000866
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	scaffold8: 41454028 .. 41458084 (+)
RNA-Seq Expression	Spg000866
Synteny	Spg000866

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAGAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTCCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTCTACCGTGGAGGAAGATCTCATGGAAAATGTCTTGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATCAAGGTGCTTCAATCAGTCAATATAAGACACCTCGATGCATAAATTCTGCGCAGATCATCGAGCTTTTAGACTCAAACGTTGTTTCCAATTATTTTAGCCAAAAATGTCCAAAATGGGATCCCAATGAATGTCACCCTCTTTTATCTTGTTAGTTGATGATGTAAGTTAATTCAAGATAAGTCCTCATGTATTTTCATGTTTGTGGTTCGTTTGCACTGTTTAGGGTGGTCGATCAATTTGCCTTCTGTGGGAGAGATTATCTTGGTTTTTTTGTTTGGTTGGCTTGGTCACTCATAACCTTTGGCTTTGAATGACCTTCTTTGAGCCACAGAATTTAAAAATAAAACTCCAATATTTAGTTTTCTGAAGAAAAATATATAGTTATAAGCTTTAATTTGAGAAAATAGTTAAATCGAAACTTCATAATGTTGTACAAAAGGTTACTCATTTAAGCCACCGAGACTATGTTTTTCAACTTTGGGCATAAGCTCTCATGATTTTGCTTTTGGTTCATCCCAAAAGGCCTCGTACCAATGGAGATACTTGTCCTCACTTATAAACCATGATCATCCCCTTTATGGCTGCATCACTCAAATGGTCCTTTTTATGTAGCCTCAACCCGTCTTTCTTTTTTGGGATATCTCTTCTACTATTCGTGCTCAGATGGATCTACAAAAAAACCATGATCATCCCCTTGTCTAGACAACGTGGGACTTTAGTTGCACTCTCTAATTTCCAACATTTCCAAAGGTTGATCTTCTAAGAACCGCTTCTTCTCCACAAAGGGAGAGACCCAATGGTGCCAATCTGTGGGTTGCGTTGCCAAAATTTAGAAGTCACTATGATCGAGAAGGAACCCATTGCACGTCTTTGATAATCTAGAGATGATTTTTCTGATTTGCTTCTCGAAATGTAAATGCATATCGGCCTTAGAATATCAAAGGAGATTGAGTTGGGTAAATCGTAGATAATAGCTAGCATTTCGAGTATTCCAAATGGACTAAATGAAAACGAACCAAATTCTCAAAATGTCTCCAATCTATCTTATTTTTCCAATTTAAAACAAGCTGAAAAATATTTCTATTTGGACCCCAATTAAAATCAGAGAACATGGATGCAAATTTGGAAGACAAGACAACCAACTCTTCTCGAACCTGTGTTGTAATGGAAGAAAATAATATATATAGATAGATCAACCCTCTCCTTTCTAGCCACAAAATACACATAAATTAGGTACATCAACACCTCTTTTTGTAGGAAGAAAATAAGAAACAATTGCTGAATTTCGACACCCTCCTCAAACGTGGGAAGATCGAATGGAAAAAAATGATCTTCCACTCTGCTCAGATACTGCTTTTACATAGGTCAAAGAGACGAGAGGAAGCCACGTGTGCGAGAACGGACAGTCAAAGCGTGGAACGGTCATAGCACGCAAAAAGACTCCCAAACCCACCTGACTGGCAACAATGGCGCACGCGTGTGGGAAAAAGCCTTGGTTTCACCCTTAACCACATTTTTAAGAGCAAGTAAGAGTGATTGTATAAAAACACTATTTCCAACTCAAAATTTATCCATATAGAACAAAGGACTCCAAACTTTACTTCTAAAGACCAAAATCAAGTCATTCCCAACATTTTTAACAAATTATCCGAGAAATCTCTTAGGCTCCGTTTGTTCATGATCTGTTATGGTAAAGCAGTTGTGATACTTATTGCAACTATGAATTGTGGTTGTTGTTGGTTGTTTTCATTCTACTTCGAACTTGATAATGGTAAGCAATCGAATGCCAAGTCTTTCTCCTAGCATGTCTTGAATTAAATTGTGGAGATCGAGTAATCAATCATGAGAGGAACACAGATGTAGTAATTAGTGAATCCAAGCTTTTTTTTTTTCATACAAATTAAGTGATAATCTTGTTCAATATATTTAGTCAAACATAAGATTAATAGACATGTATAAAGTACTTATATTATCACATGTGTAAAGTACTTGTATTATCACAAAAGAGTTAGAGGTGGTTTAAGTAACAAAATATATATCTTTTAGCGAAAAGGATATCCAAGAATTAACAGAAATTATGATAGATTGGATTCTTTAATCAAATTTATAAATTATGGTAGGATGAGATTCTTTAATCCATTAATTTCAAATTTGTCTAAAACTTTTAAATTTATCAACGGTAAGATTTCATGCATTAATTCAATTTTTGTTCTTCCCTAAATGCCCTTATTTAATTTTGATATTCTTATAAATATTGATTTTTTTTATGAACCAACCGATTATCAAATTCGGTAACTCTTATTTTATCAATTTATTTTAGCATTTTTTTTATATTAAACATTTCATTTCTGTTTTTTTTCCTAACCTTTAGCGATTTCATTTTCGTTTTTTTTTTTCTATTCTTTCAACGGGTAAAGTCAAATACGTAAATAAACTTCTTAAACTTCTTTAATATCTAGTTGTGAGCTGACAGAAACACAATTTTCATAATTTTCTATAAATTTTCCAATATTTATCCTGGCATCGAGTTTTCTATAGACATTTATCTATAAATATTATATATTTTAGTTTTCTATACACATTTATCTATAAATATTATATAATTTTAGATATGATTATTAAAAATAATAAATTATAATCTAATTAAACCACAATTAAAATTTCATAAAATCTCAGCCTCGATATTTATACAGTTTCAATATGGTCTATTATCACCTCTTTCCCCCGTTTCTTGATTACTTCTGTCTCTCATCTTCTCCACCAAAATCCTTCCCTTCTTCTTTTCCTCTTCTCAGTTTTTTTATTAGAATATGATGATTCTCCTCCCTCTGTTTCTTTATGCGATTTCTCCTTTTTCTTTTGGATAATTGTAATGGGTGGCAAAATTAGGGTAAATAATGACAAATTTAGCAATTTTTTAAAATATTTGTAAATATGGCAAAATTTTTGAATTAATTTTTTATATCCCCGTTTTGCCCTTGAATTACGTGCACACATCTTCTTGTTTTCTTCTTTCATTCGCGTGGATTTCTCTCCTCTCTTTTTGATTTGGTAGGTTTCTATCAGTGATAGAAGATTTTTATCACTGATAGAACGTTTTTCTATCACTGATAGTAGAAAAACTTTCTATCAGTGATAGAAAACTTCTATCACTGATAGTAGAAAAACTTTCTATCAGTGACAGAAAACTTCTATCACTGATAGAAAGTTAATTCTACCAGTGATAGAAGATTTTTGTCACTGATAGAATCTATCAGTGATAGAAAAACTTTCTATCAGTGATAGAAATCTTCTATCACTGATAGAATTAACTTTCTATCAGTGATAGAAAACTTTCTATCAGTCTCCGAGAAAACCGGTGAGGGGAAGGATTTAGCAGTGACGCCGGACCGATGGAACAGTCAGAGAAAGAGGCGGAAGTCGTCTTCTCCGACGTCAATCCGGTACGTGGACGACCGCGAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAACGCTGCCATCGAAACGACGGCGGTGGTCCTCTTCACCGAGGGCGGTTTCTGCTTCCACATTTCGCCGAACCAATTCCATCGATTGTCCGACAGCTTTTATATAGGGCAATTTCGGTGGGATTTTAA

mRNA sequence

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAGAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTCCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTCTACCGTGGAGGAAGATCTCATGGAAAATGTCTTGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATCAAGGTCAAAGAGACGAGAGGAAGCCACGTGTGCGAGAACGGACAGTCAAAGCGTGGAACGGTCATAGCACGCAAAAAGACTCCCAAACCCACCTGACTGGCAACAATGGCGCACGCGTGTGGGAAAAAGCCTTGGTTTCACCCTTAACCACATTTTTAAGAGCAATGATAGAAAACTTTCTATCAGTCTCCGAGAAAACCGGTGAGGGGAAGGATTTAGCAGTGACGCCGGACCGATGGAACAGTCAGAGAAAGAGGCGGAAGTCGTCTTCTCCGACGTCAATCCGGTACGTGGACGACCGCGAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAACGCTGCCATCGAAACGACGGCGGTGGTCCTCTTCACCGAGGGCGGTTTCTGCTTCCACATTTCGCCGAACCAATTCCATCGATTGTCCGACAGCTTTTATATAGGGCAATTTCGGTGGGATTTTAA

Coding sequence (CDS)

ATGGGTGACATACTTCGCGTGACAGGATTTAAAAATAAAGCTCCAAAATTTAGCTTTGTATGCAGGAAAAACACTGTTCTAAGTCTCGATGTTGAGAAAACAGATGAAGCAGAGCTCCATAATGCTGTACAAGAAGCTGCTCAACTGTTAGGCCCATTTGGAGCCACTATTGTTGACTACACTAGCTATGCAGATAGCACAACAATTCCTGGACACTATGTGTTGTACTGGGAAATTCTCCATAGGATAGAAATCTCCATTCCATCTTCAATCTATGAGGATTGTTGCCTTGTTGTTGAAGAGTCATTGAGCATATTCTACCGTGGAGGAAGATCTCATGGAAAATGTCTTGGTCCACTAGAGATAAAAATTGTAGAGGTTGGAACTTTTGAGAAACTCATGAAACGAGCAGTGAATCAAGGTCAAAGAGACGAGAGGAAGCCACGTGTGCGAGAACGGACAGTCAAAGCGTGGAACGGTCATAGCACGCAAAAAGACTCCCAAACCCACCTGACTGGCAACAATGGCGCACGCGTGTGGGAAAAAGCCTTGGTTTCACCCTTAACCACATTTTTAAGAGCAATGATAGAAAACTTTCTATCAGTCTCCGAGAAAACCGGTGAGGGGAAGGATTTAGCAGTGACGCCGGACCGATGGAACAGTCAGAGAAAGAGGCGGAAGTCGTCTTCTCCGACGTCAATCCGGTACGTGGACGACCGCGAGGTCTGCCATGGCTCGAGTACCTCGCATGTAGAGAGTCCCCATTCGTTGCAACGCTGCCATCGAAACGACGGCGGTGGTCCTCTTCACCGAGGGCGGTTTCTGCTTCCACATTTCGCCGAACCAATTCCATCGATTGTCCGACAGCTTTTATATAGGGCAATTTCGGTGGGATTTTAA

Protein sequence

MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDYTSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPLEIKIVEVGTFEKLMKRAVNQGQRDERKPRVRERTVKAWNGHSTQKDSQTHLTGNNGARVWEKALVSPLTTFLRAMIENFLSVSEKTGEGKDLAVTPDRWNSQRKRRKSSSPTSIRYVDDREVCHGSSTSHVESPHSLQRCHRNDGGGPLHRGRFLLPHFAEPIPSIVRQLLYRAISVGF

Homology

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BLAST of Spg000866 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 221.9 bits (564), Expect = 7.6e-54
Identity = 108/141 (76.60%), Postives = 121/141 (85.82%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           MGDILRVTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQEAA+LL PFGA I DY
Sbjct: 399 MGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  ISIPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIKIV+VGTFEKLM +A+N+G
Sbjct: 519 EIKIVKVGTFEKLMAQALNRG 539

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BLAST of Spg000866 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 219.2 bits (557), Expect = 4.9e-53
Identity = 107/141 (75.89%), Postives = 120/141 (85.11%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           MGDIL VTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQEAA+LL PFGA I DY
Sbjct: 399 MGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  ISIPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIKIV+VGTFEKLM +A+N+G
Sbjct: 519 EIKIVKVGTFEKLMAQALNRG 539

[Close]

BLAST of Spg000866 vs. NCBI nr
Match: XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 1.6e-51
Identity = 105/142 (73.94%), Postives = 118/142 (83.10%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDI+RVTGFK KAP FSFVCRKN VLSLD EKTDEAELHNAV+ A +LL PFGATI DY
Sbjct: 346 IGDIVRVTGFKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADY 405

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYAD++T PGHYVLYWEI  + EIS IPSSIYEDCCL VEESL+  YR  RSH K + P
Sbjct: 406 TSYADNSTFPGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINP 465

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIK+VE GTF+KL++RA  QG
Sbjct: 466 LEIKMVEAGTFDKLLERATYQG 487

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BLAST of Spg000866 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 4.6e-51
Identity = 103/142 (72.54%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           MGDILRVTGFKNK P FSF CRKN +LSLD+EKTDE +LH+AV EA+ LL P GA IVDY
Sbjct: 401 MGDILRVTGFKNKTPNFSFACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDY 460

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  R E S IPS++YEDCCL VEESLS  YR  RS+ K + P
Sbjct: 461 TSYADSSTFPGHYVLYWEIQCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINP 520

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIKIVE GTFEKLM++AV++G
Sbjct: 521 LEIKIVETGTFEKLMEQAVDRG 542

[Close]

BLAST of Spg000866 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 209.1 bits (531), Expect = 5.1e-50
Identity = 103/142 (72.54%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDI+RVTGFKNKAP F FV RKN +LSLDVEKTDEAELHNAV +   LL  FGA +VDY
Sbjct: 399 IGDIVRVTGFKNKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  + EIS IPSSIYEDCCL +EESLS  YR  RSH K + P
Sbjct: 459 TSYADSSTFPGHYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINP 518

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIKIVE  TF+KLM+R V+QG
Sbjct: 519 LEIKIVEARTFDKLMERMVDQG 540

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BLAST of Spg000866 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.6e-46
Identity = 87/141 (61.70%), Postives = 109/141 (77.30%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+LRVTGFKNKAP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL VEES +  YR GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIKIVE GTF+KLM  A++ G
Sbjct: 545 EIKIVEPGTFDKLMDYAISLG 565

[Close]

BLAST of Spg000866 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 83/141 (58.87%), Postives = 107/141 (75.89%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+L V GFKN AP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL +EESL+  YR GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIK+VE GTF+KLM  A++ G
Sbjct: 545 EIKMVESGTFDKLMDYAISLG 565

[Close]

BLAST of Spg000866 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 83/141 (58.87%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+LRV GFKNKAP FSFV R+N  LS+D +KTDE ELH AV  A Q L PFGA++V+Y
Sbjct: 415 VGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEY 474

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+ TIPGHYVL+WE L     ++P+S++E+CCL VEE+L+  YR GR+  + +GPL
Sbjct: 475 TSYADAATIPGHYVLFWE-LRAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPL 534

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EI++V  GTF+KLM  A+++G
Sbjct: 535 EIRVVAEGTFDKLMDYAISRG 554

[Close]

BLAST of Spg000866 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.3e-42
Identity = 84/143 (58.74%), Postives = 109/143 (76.22%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+LRV GFKN AP F+FV RKN  LS+D +KTDEAELH AV EA Q L PFGA++V+Y
Sbjct: 423 VGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEY 482

Query: 61  TSYAD-STTIPGHYVLYWEILHRI-EISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLG 120
           TSYAD +TTIPGHYVL+WE+        +P+S++EDCCL VEE L+  YR  R+  + +G
Sbjct: 483 TSYADTATTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIG 542

Query: 121 PLEIKIVEVGTFEKLMKRAVNQG 142
           PLEI++V  GTF+KLM  A+++G
Sbjct: 543 PLEIRVVADGTFDKLMDYALSRG 565

[Close]

BLAST of Spg000866 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-39
Identity = 80/148 (54.05%), Postives = 111/148 (75.00%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDIL+VTGF N AP+F FV RKN +LS+D +KTDEAEL  AV+ A+ LL P+GA+IV+Y
Sbjct: 426 VGDILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEY 485

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIP-------SSIYEDCCLVVEESLSIFYRGGRSH 120
           TS AD+TTIPGHYV+YWE++ R   + P         ++E CCL +EE+L+  YR GR +
Sbjct: 486 TSQADATTIPGHYVVYWELMVREGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGR-N 545

Query: 121 GKCLGPLEIKIVEVGTFEKLMKRAVNQG 142
           G+ +GPLEI++V  GTFE++M  A+++G
Sbjct: 546 GEAIGPLEIRVVRAGTFEEVMDYAISRG 572

[Close]

BLAST of Spg000866 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 2.4e-53
Identity = 107/141 (75.89%), Postives = 120/141 (85.11%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           MGDIL VTGFKNKAP FSFVCRKN VLSLD+EKTDEAELH+AVQEAA+LL PFGA I DY
Sbjct: 399 MGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TS+ADS+ IPGHYVLYWEI  +  ISIPSSIYEDCC  +EE L+  YR  RSH KC+ PL
Sbjct: 459 TSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPL 518

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIKIV+VGTFEKLM +A+N+G
Sbjct: 519 EIKIVKVGTFEKLMAQALNRG 539

[Close]

BLAST of Spg000866 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 7.7e-52
Identity = 105/142 (73.94%), Postives = 118/142 (83.10%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDI+RVTGFK KAP FSFVCRKN VLSLD EKTDEAELHNAV+ A +LL PFGATI DY
Sbjct: 346 IGDIVRVTGFKYKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADY 405

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYAD++T PGHYVLYWEI  + EIS IPSSIYEDCCL VEESL+  YR  RSH K + P
Sbjct: 406 TSYADNSTFPGHYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINP 465

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIK+VE GTF+KL++RA  QG
Sbjct: 466 LEIKMVEAGTFDKLLERATYQG 487

[Close]

BLAST of Spg000866 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 2.2e-51
Identity = 103/142 (72.54%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           MGDILRVTGFKNK P FSF CRKN +LSLD+EKTDE +LH+AV EA+ LL P GA IVDY
Sbjct: 401 MGDILRVTGFKNKTPNFSFACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDY 460

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  R E S IPS++YEDCCL VEESLS  YR  RS+ K + P
Sbjct: 461 TSYADSSTFPGHYVLYWEIQCREETSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINP 520

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIKIVE GTFEKLM++AV++G
Sbjct: 521 LEIKIVETGTFEKLMEQAVDRG 542

[Close]

BLAST of Spg000866 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 2.5e-50
Identity = 103/142 (72.54%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDI+RVTGFKNKAP F FV RKN +LSLDVEKTDEAELHNAV +   LL  FGA +VDY
Sbjct: 399 IGDIVRVTGFKNKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDY 458

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS-IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYADS+T PGHYVLYWEI  + EIS IPSSIYEDCCL +EESLS  YR  RSH K + P
Sbjct: 459 TSYADSSTFPGHYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINP 518

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEIKIVE  TF+KLM+R V+QG
Sbjct: 519 LEIKIVEARTFDKLMERMVDQG 540

[Close]

BLAST of Spg000866 vs. ExPASy TrEMBL
Match: A0A2I0HPT0 (indole-3-acetic acid-amido synthetase GH3.6 OS=Punica granatum OX=22663 GN=LOC116193173 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 9.7e-47
Identity = 89/141 (63.12%), Postives = 112/141 (79.43%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+LRV GFKNKAP+F+F+CRKN VLS+D +KTDE EL NAV+ A   L PFGAT+ +Y
Sbjct: 427 VGDVLRVAGFKNKAPQFNFICRKNVVLSIDADKTDEVELQNAVRNALNHLTPFGATVTEY 486

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+TTIPGHYVL+WE+      +IP S+YEDCCL +EESL+  YR GR+  K +GPL
Sbjct: 487 TSYADTTTIPGHYVLFWELALNGSTAIPPSVYEDCCLAIEESLNSVYRQGRASDKSIGPL 546

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EI+IVE GTF+KLM  A++ G
Sbjct: 547 EIRIVESGTFDKLMDCAISYG 567

[Close]

BLAST of Spg000866 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.3e-47
Identity = 87/141 (61.70%), Postives = 109/141 (77.30%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+LRVTGFKNKAP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL VEES +  YR GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIKIVE GTF+KLM  A++ G
Sbjct: 545 EIKIVEPGTFDKLMDYAISLG 565

[Close]

BLAST of Spg000866 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-45
Identity = 83/141 (58.87%), Postives = 107/141 (75.89%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GD+L V GFKN AP+FSF+CRKN VLS+D +KTDE EL NAV+ A   L PF A++ +Y
Sbjct: 425 VGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEY 484

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPSSIYEDCCLVVEESLSIFYRGGRSHGKCLGPL 120
           TSYAD+++IPGHYVL+WE+       IP S++EDCCL +EESL+  YR GR   K +GPL
Sbjct: 485 TSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPL 544

Query: 121 EIKIVEVGTFEKLMKRAVNQG 142
           EIK+VE GTF+KLM  A++ G
Sbjct: 545 EIKMVESGTFDKLMDYAISLG 565

[Close]

BLAST of Spg000866 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 159.1 bits (401), Expect = 5.6e-39
Identity = 75/142 (52.82%), Postives = 107/142 (75.35%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDIL+VTGF N AP+F FV RKN +LS++ +KTDEAEL +AV+ A+ LLG  G  +++Y
Sbjct: 411 VGDILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEY 470

Query: 61  TSYADSTTIPGHYVLYWEILHRIEISIPS-SIYEDCCLVVEESLSIFYRGGRSHGKCLGP 120
           TSYA++ TIPGHYV+YWE+L + + + P+  +   CCL +EESL+  YR  R   K +GP
Sbjct: 471 TSYAETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGP 530

Query: 121 LEIKIVEVGTFEKLMKRAVNQG 142
           LEI++V+ GTFE+LM  A+++G
Sbjct: 531 LEIRVVKNGTFEELMDYAISRG 552

[Close]

BLAST of Spg000866 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.3e-38
Identity = 74/143 (51.75%), Postives = 107/143 (74.83%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDILRVTGF N AP+F F+ R+N +LS++ +KTDEA+L  AV+ A++LL   G  +++Y
Sbjct: 412 VGDILRVTGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEY 471

Query: 61  TSYADSTTIPGHYVLYWEILHRIEI-SIPS-SIYEDCCLVVEESLSIFYRGGRSHGKCLG 120
           TSYAD+ TIPGHYV+YWE+L R +  ++PS  +   CCL +EESL+  YR  R   K +G
Sbjct: 472 TSYADTKTIPGHYVIYWELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIG 531

Query: 121 PLEIKIVEVGTFEKLMKRAVNQG 142
           PLEI++V+ GTFE+LM  ++++G
Sbjct: 532 PLEIRVVQNGTFEELMDFSISRG 554

[Close]

BLAST of Spg000866 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.8e-38
Identity = 73/143 (51.05%), Postives = 103/143 (72.03%), Query Frame = 0


Query: 1   MGDILRVTGFKNKAPKFSFVCRKNTVLSLDVEKTDEAELHNAVQEAAQLLGPFGATIVDY 60
           +GDILRVTGF N AP+F F+ RKN +LS++ +KTDEAEL  AV+ A++L    G  +++Y
Sbjct: 418 VGDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEY 477

Query: 61  TSYADSTTIPGHYVLYWEILHRIEIS--IPSSIYEDCCLVVEESLSIFYRGGRSHGKCLG 120
           TSYA++ TIPGHYV+YWE+L R + +  +   +   CCL +EESL+  YR  R   K +G
Sbjct: 478 TSYAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIG 537

Query: 121 PLEIKIVEVGTFEKLMKRAVNQG 142
           PLEI++V  GTFE+LM  A+++G
Sbjct: 538 PLEIRVVRNGTFEELMDYAISRG 560

The following BLAST results are available for this feature:

BLAST of Spg000866 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570863.1	7.6e-54	76.60	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	4.9e-53	75.89	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_022140614.1	1.6e-51	73.94	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_022140368.1	4.6e-51	72.54	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]
XP_022140778.1	5.1e-50	72.54	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]

BLAST of Spg000866 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	4.6e-46	61.70	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	1.9e-44	58.87	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	1.3e-43	58.87	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	5.3e-42	58.74	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
P0C0M2	1.9e-39	54.05	Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo...	[more]

BLAST of Spg000866 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUU5	2.4e-53	75.89	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CG61	7.7e-52	73.94	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CHU9	2.2e-51	72.54	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CIS9	2.5e-50	72.54	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A2I0HPT0	9.7e-47	63.12	indole-3-acetic acid-amido synthetase GH3.6 OS=Punica granatum OX=22663 GN=LOC11...	[more]

BLAST of Spg000866 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	3.3e-47	61.70	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.4e-45	58.87	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	5.6e-39	52.82	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	1.3e-38	51.75	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	4.8e-38	51.05	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 1..143 e-value: 3.7E-41 score: 141.6
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 1..142
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 210..233
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 245..264
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 1..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000866.1	Spg000866.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity