Spg000798 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg000798 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 366.7 bits (940), Expect = 2.3e-97
Identity = 216/401 (53.87%), Postives = 248/401 (61.85%), Query Frame = 0

Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------------- 60
           M H VE+ +DEKDK+ALQHIEDVTS ADEIQ+QIL EILSTNA+VE              
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  ------------------------------CS------------SGGEHKLIPMFEREFV 120
                                         CS            SGGE KLIPM+E EFV
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RRLPFLNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSN 180
           RRL F NY+MPRMK+LFPN N  KGKGM F FA+ E   KAGI+VRSI TS +KRS++  
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLK 180

Query: 181 NIPIGDNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWV 240
           + PIG+NTSP+DIILCTD +QSLY QLLCGLYQN++VFR+GA+FASTL+HV K LENHWV
Sbjct: 181 SKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWV 240

Query: 241 LLANDIRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIE------------------TE 284
           +LANDI TG++NP ITN SVRESVM I +KPNP++AD IE                  T+
Sbjct: 241 VLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300

BLAST of Spg000798 vs. NCBI nr
Match: XP_004140044.1 (indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN46710.1 hypothetical protein Csa_021088 [Cucumis sativus])

HSP 1 Score: 339.0 bits (868), Expect = 5.0e-89
Identity = 200/397 (50.38%), Postives = 239/397 (60.20%), Query Frame = 0

Query: 6   ENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------- 65
           E  +DEK+K+ALQ+IED+T+ ADEIQ+QIL EILSTNA+VE                   
Sbjct: 6   EKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSPTFKKL 65

Query: 66  -------------------------CS------------SGGEHKLIPMFEREFVRRLPF 125
                                    CS            SGGEHKLIPM+E+EFVRRL F
Sbjct: 66  IPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFVRRLSF 125

Query: 126 LNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIG 185
            +Y+MPRMK+LFP+ N HK KG+ F FA+ E   K GI+VRSI T+ +KRS++  ++P G
Sbjct: 126 FSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLESMPSG 185

Query: 186 DNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLAND 245
           +NTSP+DIILCTD YQSLY QLLCGLYQNE+VFRV A+FASTL+HV K LENHWV LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWVDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNIL-KPNPQLADFIETEY---------------------- 284
           IRT T+NP ITNSSVRES+M I+ KPNP++AD IE E                       
Sbjct: 246 IRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAKYINAI 305

BLAST of Spg000798 vs. NCBI nr
Match: XP_008448232.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0062188.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa] >TYK04840.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 338.6 bits (867), Expect = 6.6e-89
Identity = 198/396 (50.00%), Postives = 234/396 (59.09%), Query Frame = 0

Query: 6   ENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------- 65
           E  +DEKDK+ALQ+IED+T+ ADEIQ+QIL EILS NA+VE                   
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  -------------------------CS------------SGGEHKLIPMFEREFVRRLPF 125
                                    CS            SG EHKLIPM+E+EFVRRL F
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 LNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIG 185
            +Y+ PRMK+LFPN N HK KG+ F FA+ E   K GIVVRSI T+ +KRS++  ++P G
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 DNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLAND 245
           +NTSP+DIILCTD YQSLY QLLCGLYQN++VFRV A+FASTL+HV K LENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETEY---------------------- 284
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE E                       
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

BLAST of Spg000798 vs. NCBI nr
Match: KAG7010712.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-87
Identity = 203/411 (49.39%), Postives = 240/411 (58.39%), Query Frame = 0

Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------------- 60
           M H  E   DE+DKQALQHIEDVT++A +IQ+QIL +ILSTNA VE              
Sbjct: 15  MAHVDEISSDERDKQALQHIEDVTTHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 74

Query: 61  ------------------------------C------------SSGGEHKLIPMFEREFV 120
                                         C            +SGGE KLIPM+E EF+
Sbjct: 75  TFKNLIPLIAYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPMYEEEFM 134

Query: 121 RRLPFLNYVMPRMKELFPNNL-HKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSN 180
           RRL F +YVMPRMK+LFPN+L  KGKGM+F FA+ EL   +GIV  + +TS  KR+N   
Sbjct: 135 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTTSGIVAHTATTSLCKRANL-- 194

Query: 181 NIPIGDNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWV 240
                +NTSP DIILCTD  QSLY QLLCGL QN++VFRVGA+FASTL+HV+K LE+HWV
Sbjct: 195 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDVVFRVGALFASTLIHVLKFLEDHWV 254

Query: 241 LLANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETEY------------------ 295
           +LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE E                   
Sbjct: 255 VLASDIRAGTLNTKITDPSVRETVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 314

BLAST of Spg000798 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 327.4 bits (838), Expect = 1.5e-85
Identity = 199/400 (49.75%), Postives = 232/400 (58.00%), Query Frame = 0

Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------------- 60
           M H  E   DE+DKQALQHIEDVT +A +IQ+QIL +ILSTNA VE              
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  ------------------------------C------------SSGGEHKLIPMFEREFV 120
                                         C            +SGGE KLIPM+E EF+
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLPFLNYVMPRMKELFPNNL-HKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSN 180
           RRL F +YVMPRMK+LFPN+L  KGKGM+F FA+ EL  ++GIV  + +TS  KR+N   
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 NIPIGDNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWV 240
                +NTSP DIILCTD  QSLY QLLCGL QN+IVFRVGA+FASTL+HV+K LE+HWV
Sbjct: 181 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWV 240

Query: 241 LLANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETEY------------------ 284
           +LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE E                   
Sbjct: 241 VLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 300

BLAST of Spg000798 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-65
Identity = 164/402 (40.80%), Postives = 201/402 (50.00%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------------ 70
           EK+K  LQ IEDVT+NAD++Q+++L EILS NA VE                        
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 71  --------------------CS------------SGGEHKLIPMFEREFVRRLPFLNYVM 130
                               CS            SGGE KL+P  E E  RR    + +M
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNN--IPIGDNTS 190
           P M +  P  L KGKGMYFLF ++E     G+  R + TS+YK S+  N    P  + TS
Sbjct: 139 PVMDQFVP-GLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTS 198

Query: 191 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 250
           PN  ILC+D YQS+YSQ+LCGL Q++ V RVGA+FAS  +  +K LE HW  LA DIRTG
Sbjct: 199 PNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTG 258

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETE---------------------------- 284
           TL+  IT+SSVRE+V  ILKP+P+LADF+E+E                            
Sbjct: 259 TLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTM 318

BLAST of Spg000798 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.9e-65
Identity = 159/404 (39.36%), Postives = 203/404 (50.25%), Query Frame = 0

Query: 9   MDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVEC--------------------- 68
           +D+K+KQ LQ IE++TSNAD++Q+Q+L EIL+ NA VE                      
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  -----------------------------------SSGGEHKLIPMFEREFVRRLPFLNY 128
                                              +SGGE KL+P  E E  RR    + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNN--IPIGDN 188
           +MP M +  P  L  GKGMYFLF ++E     G+  R + TS+YK S+       P  + 
Sbjct: 137 LMPVMSQFVP-GLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIR 248
           TSPN+ ILC+D YQS+YSQ+LCGL Q++ V RVGA+FAS  +  +K LE HW+ L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTLNPTITNSSVRESVMNILKPNPQLADFIE------------------TEY------- 284
           TGTL+  IT+ SVRE+V  ILKP+P+LADF+E                  T+Y       
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

BLAST of Spg000798 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.8e-50
Identity = 133/389 (34.19%), Postives = 179/389 (46.02%), Query Frame = 0

Query: 15  QALQHIEDVTSNADEIQQQILAEILSTNAHVEC--------------------------- 74
           +AL+ IE VT+NA ++Q+++L EIL+ NA  E                            
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  ------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVMPRM 134
                                         +SGGE KL+P    E  RR    + +MP M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSN--SSNNIPIGDNTSPND 194
            +   + L KGK MY LF +AE     G+  R + TS+Y+          P    TSP++
Sbjct: 142 SQSV-SGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDE 201

Query: 195 IILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTGTLN 254
            ILC D YQS+Y+QLLCGL     V RVGA+FAS  +  +  LE HW  L +DIRTG L+
Sbjct: 202 AILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELD 261

Query: 255 PTITNSSVRESVMNILKPNPQLADFIETE------------------------------- 284
           P IT+  VR++V  +L+ +P LAD IE E                               
Sbjct: 262 PEITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQY 321

BLAST of Spg000798 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.1e-49
Identity = 134/391 (34.27%), Postives = 182/391 (46.55%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------C---------------- 70
           EKD +AL+ IE++T NAD +Q+ +LAEIL+ NA  E       C                
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  ---------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVM 130
                                            +S GE KL+P  + E  RR    + +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSN--SSNNIPIGDNTS 190
           P M  L+   L KGKGMYFLF ++E     G+  R + TS+YK  +  S    P    TS
Sbjct: 137 PVM-NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 250
           PN+ ILC D +QS+Y+Q+LCGL     V RVGA+FAS L+  ++ L+ HW   A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETE---------------------------- 284
            L+  IT+ S+R+ +  ILKP+P LA+FI  E                            
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

BLAST of Spg000798 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 5.8e-48
Identity = 128/391 (32.74%), Postives = 180/391 (46.04%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE----C------------------- 70
           E D + L+ I+++T+N D +Q+++L EIL  NA  E    C                   
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 71  ---------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVM 130
                                            +S GE KL+P    E  RR    + +M
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNN--IPIGDNTS 190
           P M  L+   L KGKG+YFLF ++E     G+  R + TS+YK  +  N    P  + TS
Sbjct: 144 PVM-NLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTS 203

Query: 191 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 250
           P   ILC D +QS+Y+Q++CGL Q   V R+GA+FAS L+  ++ L+ +W  LA+DI +G
Sbjct: 204 PTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESG 263

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETE---------------------------- 284
            L P +T+ SVRE+V  IL P+P+LA  I  E                            
Sbjct: 264 ELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAM 323

BLAST of Spg000798 vs. ExPASy TrEMBL
Match: A0A0A0KAF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.4e-89
Identity = 200/397 (50.38%), Postives = 239/397 (60.20%), Query Frame = 0

Query: 6   ENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------- 65
           E  +DEK+K+ALQ+IED+T+ ADEIQ+QIL EILSTNA+VE                   
Sbjct: 6   EKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSPTFKKL 65

Query: 66  -------------------------CS------------SGGEHKLIPMFEREFVRRLPF 125
                                    CS            SGGEHKLIPM+E+EFVRRL F
Sbjct: 66  IPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFVRRLSF 125

Query: 126 LNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIG 185
            +Y+MPRMK+LFP+ N HK KG+ F FA+ E   K GI+VRSI T+ +KRS++  ++P G
Sbjct: 126 FSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLESMPSG 185

Query: 186 DNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLAND 245
           +NTSP+DIILCTD YQSLY QLLCGLYQNE+VFRV A+FASTL+HV K LENHWV LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWVDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNIL-KPNPQLADFIETEY---------------------- 284
           IRT T+NP ITNSSVRES+M I+ KPNP++AD IE E                       
Sbjct: 246 IRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAKYINAI 305

BLAST of Spg000798 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 3.2e-89
Identity = 198/396 (50.00%), Postives = 234/396 (59.09%), Query Frame = 0

Query: 6   ENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------- 65
           E  +DEKDK+ALQ+IED+T+ ADEIQ+QIL EILS NA+VE                   
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  -------------------------CS------------SGGEHKLIPMFEREFVRRLPF 125
                                    CS            SG EHKLIPM+E+EFVRRL F
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 LNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIG 185
            +Y+ PRMK+LFPN N HK KG+ F FA+ E   K GIVVRSI T+ +KRS++  ++P G
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 DNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLAND 245
           +NTSP+DIILCTD YQSLY QLLCGLYQN++VFRV A+FASTL+HV K LENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETEY---------------------- 284
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE E                       
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

BLAST of Spg000798 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 3.2e-89
Identity = 198/396 (50.00%), Postives = 234/396 (59.09%), Query Frame = 0

Query: 6   ENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------- 65
           E  +DEKDK+ALQ+IED+T+ ADEIQ+QIL EILS NA+VE                   
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  -------------------------CS------------SGGEHKLIPMFEREFVRRLPF 125
                                    CS            SG EHKLIPM+E+EFVRRL F
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 LNYVMPRMKELFPN-NLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIG 185
            +Y+ PRMK+LFPN N HK KG+ F FA+ E   K GIVVRSI T+ +KRS++  ++P G
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 DNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLAND 245
           +NTSP+DIILCTD YQSLY QLLCGLYQN++VFRV A+FASTL+HV K LENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETEY---------------------- 284
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE E                       
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

BLAST of Spg000798 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 7.3e-86
Identity = 199/400 (49.75%), Postives = 232/400 (58.00%), Query Frame = 0

Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------------- 60
           M H  E   DE+DKQALQHIEDVT +A +IQ+QIL +ILSTNA VE              
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  ------------------------------C------------SSGGEHKLIPMFEREFV 120
                                         C            +SGGE KLIPM+E EF+
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLPFLNYVMPRMKELFPNNL-HKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSN 180
           RRL F +YVMPRMK+LFPN+L  KGKGM+F FA+ EL  ++GIV  + +TS  KR+N   
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 NIPIGDNTSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWV 240
                +NTSP DIILCTD  QSLY QLLCGL QN+IVFRVGA+FASTL+HV+K LE+HWV
Sbjct: 181 -----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWV 240

Query: 241 LLANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETEY------------------ 284
           +LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE E                   
Sbjct: 241 VLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKY 300

BLAST of Spg000798 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 2.4e-84
Identity = 191/391 (48.85%), Postives = 223/391 (57.03%), Query Frame = 0

Query: 9   MDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE---------------------- 68
           +DEKDKQALQ +EDVT  ADEIQ+++L EILSTNAHVE                      
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ----------------------C------------SSGGEHKLIPMFEREFVRRLPFLNY 128
                                 C            +SGGE KLIP  E+EF R+L   NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VMPRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNNIPIGDNTS 188
           + PRMK+LFP  LH GK M+F FA+ E   KAGIV RS+STSF K  + +N       TS
Sbjct: 124 MRPRMKQLFP-GLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 183

Query: 189 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 248
           P DI+ CTD YQSLY QLLCGL QN+ V RVG++FASTL+++ K LENHWVLLANDIRTG
Sbjct: 184 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 243

Query: 249 TLNPTITNSSVRESVMNILKPNPQLADFIETEY--------------------------- 284
           T+N  I +S VR+SVMNILKP+PQLADFIE+E                            
Sbjct: 244 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 303

BLAST of Spg000798 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.5e-67
Identity = 164/402 (40.80%), Postives = 201/402 (50.00%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE------------------------ 70
           EK+K  LQ IEDVT+NAD++Q+++L EILS NA VE                        
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 71  --------------------CS------------SGGEHKLIPMFEREFVRRLPFLNYVM 130
                               CS            SGGE KL+P  E E  RR    + +M
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNN--IPIGDNTS 190
           P M +  P  L KGKGMYFLF ++E     G+  R + TS+YK S+  N    P  + TS
Sbjct: 139 PVMDQFVP-GLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTS 198

Query: 191 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 250
           PN  ILC+D YQS+YSQ+LCGL Q++ V RVGA+FAS  +  +K LE HW  LA DIRTG
Sbjct: 199 PNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTG 258

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETE---------------------------- 284
           TL+  IT+SSVRE+V  ILKP+P+LADF+E+E                            
Sbjct: 259 TLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTM 318

BLAST of Spg000798 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.9e-66
Identity = 159/404 (39.36%), Postives = 203/404 (50.25%), Query Frame = 0

Query: 9   MDEKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVEC--------------------- 68
           +D+K+KQ LQ IE++TSNAD++Q+Q+L EIL+ NA VE                      
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  -----------------------------------SSGGEHKLIPMFEREFVRRLPFLNY 128
                                              +SGGE KL+P  E E  RR    + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSNN--IPIGDN 188
           +MP M +  P  L  GKGMYFLF ++E     G+  R + TS+YK S+       P  + 
Sbjct: 137 LMPVMSQFVP-GLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIR 248
           TSPN+ ILC+D YQS+YSQ+LCGL Q++ V RVGA+FAS  +  +K LE HW+ L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTLNPTITNSSVRESVMNILKPNPQLADFIE------------------TEY------- 284
           TGTL+  IT+ SVRE+V  ILKP+P+LADF+E                  T+Y       
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

BLAST of Spg000798 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.2e-50
Identity = 134/391 (34.27%), Postives = 182/391 (46.55%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVE-------C---------------- 70
           EKD +AL+ IE++T NAD +Q+ +LAEIL+ NA  E       C                
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  ---------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVM 130
                                            +S GE KL+P  + E  RR    + +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSN--SSNNIPIGDNTS 190
           P M  L+   L KGKGMYFLF ++E     G+  R + TS+YK  +  S    P    TS
Sbjct: 137 PVM-NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTG 250
           PN+ ILC D +QS+Y+Q+LCGL     V RVGA+FAS L+  ++ L+ HW   A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETE---------------------------- 284
            L+  IT+ S+R+ +  ILKP+P LA+FI  E                            
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

BLAST of Spg000798 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.5e-46
Identity = 127/394 (32.23%), Postives = 184/394 (46.70%), Query Frame = 0

Query: 11  EKDKQALQHIEDVTSNADEIQQQILAEILSTNAHVEC----------------------- 70
           E + +AL+ IE++T N D +Q+++L EILS N++ E                        
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  ---------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVM 130
                                            +S GE KL+P  E +  RR    N +M
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 PRMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYK----RSNSSNNIPIGDN 190
           P M  L+   L KGKG+YFLF ++E T   G+  R   TS+YK    R++ S+++     
Sbjct: 138 PVM-NLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSV----Y 197

Query: 191 TSPNDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIR 250
           TSP + ILC D  QS+Y+Q+LCGL     V R+GA+F S L+  +  L+N+W  L+ DI 
Sbjct: 198 TSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIS 257

Query: 251 TGTLNPTITNSSVRESVMNIL-KPNPQLADFI------------------ETEY------ 284
           TGTL+  I + +++  + NIL KP+ +LA+F+                   T+Y      
Sbjct: 258 TGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVT 317

BLAST of Spg000798 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.3e-46
Identity = 128/392 (32.65%), Postives = 179/392 (45.66%), Query Frame = 0

Query: 12  KDKQALQHIEDVTSNADEIQQQILAEILSTNAHVEC------------------------ 71
           KD +AL+ IE++T N D +Q++++ EILS N+  E                         
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  --------------------------------SSGGEHKLIPMFEREFVRRLPFLNYVMP 131
                                           +S GE KL+P  + +  RR    + +MP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 RMKELFPNNLHKGKGMYFLFARAELTIKAGIVVRSISTSFYKRSNSSN--NIPIGDNTSP 191
            M  L+   L KGK +YFLF + E     G+  R + TS+YK        N P    TSP
Sbjct: 138 VM-NLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSP 197

Query: 192 NDIILCTDPYQSLYSQLLCGLYQNEIVFRVGAIFASTLVHVVKLLENHWVLLANDIRTGT 251
           N+ ILC D  QS+Y+Q+LCGL     V R+GA+FAS L+  +  L+ +W  LA+DI TGT
Sbjct: 198 NEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGT 257

Query: 252 LNPTITNSSVRESVMNIL-KPNPQLADFI-------------------ETEY-------- 284
           L+  I++ +++ES+  IL KP+ +LADFI                    T+Y        
Sbjct: 258 LSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGA 317