Spg000749 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000749
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold8: 47645821 .. 47649047 (+)
RNA-Seq Expression	Spg000749
Synteny	Spg000749

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTGCAAAAGCCAATACTGTGTCACTAATAAAGACGAAGCGCAGCCCCATTTCAAAAAGGTTTCTAAAATATATATTCCCATCCCCACGAACCCTTCGCAAGGTGTCGCGCCACTGTCTCACGAGCTCCTTTTCTTTGTTCATTTCCCCCATTTCTTTATTCTCAATTCTCTGTTTGAATTGAACCAAATGTTTTCCACAAAGGGATCGATCACATTCATCAGCCCATCCACCAGCAACCAAATATTTTGACTCTCCAGCGTTTTGTTGTGCCAATCAAAGGGCGACACCAGTTCAACCCTCGCGGGAGTTGCTCCACCTCACAACCCACCCTCTTGTTCTTGTCTTTCGATTTTCTTTTGGGGCCCAAGAATTATGCAATTATTGGATTGTTGAGCGGCGTAAAGTTGAATATTCAACCATTTGGGGTTCAGAATCTTTTCTCCCTGCTGCATTTCTCTACCATTTTGCACTTCTCCTGCTGTTTTCTGGTCGATCAGTTTCAGTACCTTCAAGTTTTCCATCTGGGTACGGCGGTTTCTTTGCCTTCTTTTCATGTTTGTTCACCTCTGCTTTGCTCTGCTGTTGATTCTTCAAAATGCGACTTGTTTAATTTCGTAGTTGTCGTATGTTTTCAACATTGTTTATTAGTGTGCTGCATTCTGGTTATGTTCGTGGCTGTTTTTAACAATTCGAAACCATGGTGAATTTGTCTGTCCCTCTCCTTCTTGATTGTTAACTAAAGTGGTTTAGATGGAAAAATGTTGGCTTCCTGTCAATCAGTTTTAAACGAAGGTGATTTAAAATCAGTTCTATTAATAGATAAATTGTTGATTTGTTTTTGTTTCCTTATATTCTACTTTTCTGTTTATAGATTCTTATCCCAACACAGATGCCAAACTCAAAAAAGAGGAAAAGAAAAAAAGAATTTTGGTGCTTCTGTTGGGTTTTGTTTTCTTGCTTGTTGTACTTGTACCAATAAATGATACAACTTTAGTTTCTCACTCTTAAACCCACTTTTCTGTTGTACAAGCAAACAATGATTCCATCTAATTATGACTGCCATGTTATTGTAAATATTAATCCAAACATGGATTTTCGATTGAAAGAACCTTGAAGATTCTTCCTCATAGTTTCCATTTCCAACGGGTTCTGTCCTCCCTATTCTATTGTAAGATACACCTTGCTTTTATGATGTATTTATAGAAGCATTGAATGATCTGAAACTTAATTGTTTTTACTTCTTGTACAATGTTCATGTAGTGCTTTCACATTTCATATTGCTTCTTCTTTCTGGATATGAATTTGATATAGGCAAGTAATAACTATATGCAGCAGTACACCTTTATAGATCGATTGTTCTTGATTTGTAATTTCTGGAGATTCATGATTTGAATCTTTATTCTGTTATTATGCAATTTGGATTGCACCTTTGAAAACAAATATCATTGAAAGTGAGGTTTGAATAGACTCCACATTGCTGATGTGAGATCTTCCTACAAAATTGACTGGTCCGTGGAATCAGTTTTGTGATGCTTGCATAACATAAGTTTCAATGGAACCAATTATAAGTTCTTCAAAGAAGTTCCTCCGGCAATCACACTTCTTTTTTTGACTAAATATCAGATATGCTGGCATGAGTACTGTAGACTACAGAGAGAACGGAATGTATCAGCAGCAGAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACTGAGCTGCGCCTTACATCGATAGATTCTGAAGCAGCAACTTCTCGTTCAGAGTCCAAAGGTGTCTCTTTCAGGAAAGCTTCCACTGGTGGTTTTGTCAGGAATCGTAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTATCATATTCTAATTTTGATGAGTTAAATCTAGCCAATAAGGAAAGTAGAAACGAAAGCATTTAATTTGAGACCCTGAATTCAAAAGTTTAATGCCTAGAAGCCTCATTCATTATTGAGCAAATGGAAGGCTATTCTGTTTAACCATTACATTGCATTGTGACTGAGTTATTCACATTTGAGGTAACTCTAATACATAACAGCTAATATAGTTTTATGCTTTAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACCTGCCGCCACGAGCTTGGGAACATAAGGTGCCTGGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTATGTACGAAAACCTAGGTTGCATTGACATATTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATTCCGCCCGTACAACTGTCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGAAGATCACGAGGTCGATATGCATGATGGATGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTTACTGTAAGATCTCTCACTGTCTCTCTGAGTTAATGAATGGAATCTCTTGCACAAACTCAGATTTTTGTGTATGGAAATGCAAAGCAATTGCCATCAAATTTCAGATTCTTAGCAACAAAAATAAACAAGCACTTCATCATGCTAAAATCCTTTGTTTTTCATTTGATGTAATGCAGGTTTTTAACTGCTTTGGACGACAGTTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCGGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGATGATGATGCGAAACAATTTTCTTATAGTCTGGAAGTTGGTGGGAATGGCAGAAAACTCATCTGGCAAGGTATGCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCATGATGGATTAATCATTCAGAGGAACTTGGCACTTTTCTGCTCTGGAAATGAAGGGCAGGAATTGAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGAGAACAAATATACATTCTCAAGGTCAAAAGTTGTACAAATATGTGATAATTACCTCTGTAAATGGCTGCTACCATTCAGCCATTTTAGAAGCTGATAATTTCCTCACCTACATTTTCTTCTTCTTTTTTGTGTATATTATTTTTGCTTCTAATATCCATGAGGAAATTACAAAAATAGCATGATAAAAGTAACGTTTCATTCACAATAATG

mRNA sequence

ATGGCCTCTGCAAAAGCCAATACTGTGTCACTAATAAAGACGAAGCGCAGCCCCATTTCAAAAAGGGCGACACCAGTTCAACCCTCGCGGGAGTTGCTCCACCTCACAACCCACCCTCTTGTTCTTGTCTTTCGATTTTCTTTTGGGGCCCAAGAATTATGCAATTATTGGATTGTTGAGCGGCGTAAAGTTGAATATTCAACCATTTGGGGTTCAGAATCTTTTCTCCCTGCTGCATTTCTCTACCATTTTGCACTTCTCCTGCTGTTTTCTGGTCGATCAGTTTCAGTACCTTCAAGTTTTCCATCTGGGTACGGCGGTTTCTTTGCCTTCTTTTCATTGCTTTCACATTTCATATTGCTTCTTCTTTCTGGATATGAATTTGATATAGGCAAATATGCTGGCATGAGTACTGTAGACTACAGAGAGAACGGAATGTATCAGCAGCAGAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACTGAGCTGCGCCTTACATCGATAGATTCTGAAGCAGCAACTTCTCGTTCAGAGTCCAAAGGTGTCTCTTTCAGGAAAGCTTCCACTGGTGGTTTTGTCAGGAATCGTAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACCTGCCGCCACGAGCTTGGGAACATAAGGTGCCTGGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTATGTACGAAAACCTAGGTTGCATTGACATATTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATTCCGCCCGTACAACTGTCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGAAGATCACGAGGTCGATATGCATGATGGATGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTTACTGTTTTTAACTGCTTTGGACGACAGTTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCGGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGATGATGATGCGAAACAATTTTCTTATAGTCTGGAAGTTGGTGGGAATGGCAGAAAACTCATCTGGCAAGGTATGCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCATGATGGATTAATCATTCAGAGGAACTTGGCACTTTTCTGCTCTGGAAATGAAGGGCAGGAATTGAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGA

Coding sequence (CDS)

ATGGCCTCTGCAAAAGCCAATACTGTGTCACTAATAAAGACGAAGCGCAGCCCCATTTCAAAAAGGGCGACACCAGTTCAACCCTCGCGGGAGTTGCTCCACCTCACAACCCACCCTCTTGTTCTTGTCTTTCGATTTTCTTTTGGGGCCCAAGAATTATGCAATTATTGGATTGTTGAGCGGCGTAAAGTTGAATATTCAACCATTTGGGGTTCAGAATCTTTTCTCCCTGCTGCATTTCTCTACCATTTTGCACTTCTCCTGCTGTTTTCTGGTCGATCAGTTTCAGTACCTTCAAGTTTTCCATCTGGGTACGGCGGTTTCTTTGCCTTCTTTTCATTGCTTTCACATTTCATATTGCTTCTTCTTTCTGGATATGAATTTGATATAGGCAAATATGCTGGCATGAGTACTGTAGACTACAGAGAGAACGGAATGTATCAGCAGCAGAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACTGAGCTGCGCCTTACATCGATAGATTCTGAAGCAGCAACTTCTCGTTCAGAGTCCAAAGGTGTCTCTTTCAGGAAAGCTTCCACTGGTGGTTTTGTCAGGAATCGTAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACCTGCCGCCACGAGCTTGGGAACATAAGGTGCCTGGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTATGTACGAAAACCTAGGTTGCATTGACATATTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATTCCGCCCGTACAACTGTCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGAAGATCACGAGGTCGATATGCATGATGGATGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTTACTGTTTTTAACTGCTTTGGACGACAGTTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCGGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGATGATGATGCGAAACAATTTTCTTATAGTCTGGAAGTTGGTGGGAATGGCAGAAAACTCATCTGGCAAGGTATGCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCATGATGGATTAATCATTCAGAGGAACTTGGCACTTTTCTGCTCTGGAAATGAAGGGCAGGAATTGAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGA

Protein sequence

MASAKANTVSLIKTKRSPISKRATPVQPSRELLHLTTHPLVLVFRFSFGAQELCNYWIVERRKVEYSTIWGSESFLPAAFLYHFALLLLFSGRSVSVPSSFPSGYGGFFAFFSLLSHFILLLLSGYEFDIGKYAGMSTVDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKASTGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIWRVR

Homology

[Close]

BLAST of Spg000749 vs. NCBI nr
Match: XP_038900383.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-183
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0


Query: 136 MSTVDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKAS 195
           M+TV++RE GMYQQQKSREEHA SAASFCKEVTELRLTSIDS+AATSR E KG++FRKAS
Sbjct: 13  MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 72

Query: 196 TGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 255
           TGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIR
Sbjct: 73  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIR 132

Query: 256 CLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPY 315
           CLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIPY
Sbjct: 133 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPY 192

Query: 316 LVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 375
           LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 193 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 252

Query: 376 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRN 435
           PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR 
Sbjct: 253 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 312

Query: 436 LALFCSGNEGQELKLKVAGRIWRV 460
           LALFCSGNEG ELKLKV GRIWRV
Sbjct: 313 LALFCSGNEGMELKLKVTGRIWRV 336

[Close]

BLAST of Spg000749 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-183
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0


Query: 136 MSTVDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKAS 195
           M+TV++RE GMYQQQKSREEHA SAASFCKEVTELRLTSIDS+AATSR E KG++FRKAS
Sbjct: 1   MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 60

Query: 196 TGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 255
           TGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIR
Sbjct: 61  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIR 120

Query: 256 CLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPY 315
           CLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIPY
Sbjct: 121 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPY 180

Query: 316 LVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 375
           LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 181 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 240

Query: 376 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRN 435
           PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR 
Sbjct: 241 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 300

Query: 436 LALFCSGNEGQELKLKVAGRIWRV 460
           LALFCSGNEG ELKLKV GRIWRV
Sbjct: 301 LALFCSGNEGMELKLKVTGRIWRV 324

[Close]

BLAST of Spg000749 vs. NCBI nr
Match: XP_038900382.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-183
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0


Query: 136 MSTVDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKAS 195
           M+TV++RE GMYQQQKSREEHA SAASFCKEVTELRLTSIDS+AATSR E KG++FRKAS
Sbjct: 13  MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 72

Query: 196 TGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 255
           TGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIR
Sbjct: 73  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIR 132

Query: 256 CLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPY 315
           CLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIPY
Sbjct: 133 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPY 192

Query: 316 LVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 375
           LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 193 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 252

Query: 376 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRN 435
           PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR 
Sbjct: 253 PVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 312

Query: 436 LALFCSGNEGQELKLKVAGRIWRV 460
           LALFCSGNEG ELKLKV GRIWRV
Sbjct: 313 LALFCSGNEGMELKLKVTGRIWRV 336

[Close]

BLAST of Spg000749 vs. NCBI nr
Match: XP_008449780.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-180
Identity = 300/326 (92.02%), Postives = 314/326 (96.32%), Query Frame = 0


Query: 136 MST-VDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKA 195
           MST +++RE GMYQQQKSREEHASSAASFCKEVTEL LTSIDS+ ATSR E KGV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 196 STGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 255
           STGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 256 RCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIP 315
           RCLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 316 YLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 375
            LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 376 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 435
           APVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 436 NLALFCSGNEGQELKLKVAGRIWRVR 461
            LALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Spg000749 vs. NCBI nr
Match: XP_031740164.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetical protein Csa_018085 [Cucumis sativus])

HSP 1 Score: 642.5 bits (1656), Expect = 2.8e-180
Identity = 300/326 (92.02%), Postives = 313/326 (96.01%), Query Frame = 0


Query: 136 MST-VDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKA 195
           MST +++RE GMYQQ KSREEHASSAASFCKEVTELRLTSIDS+ ATSR E KGV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 196 STGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 255
           STGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 256 RCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIP 315
           RCLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 316 YLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 375
            LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 376 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 435
           APVYMAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 436 NLALFCSGNEGQELKLKVAGRIWRVR 461
            LALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Spg000749 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 5.9e-133
Identity = 219/301 (72.76%), Postives = 252/301 (83.72%), Query Frame = 0


Query: 162 SFCKEVTELRLTSIDSEAATSRSESKGVSFRKASTGGF----VRNRNDVHELLDCTVCMN 221
           S  KEV E   T +D E  T++ E       K  + G     + + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 222 LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGC 281
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 282 IDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHR 341
            DIFPYYSKLKHE++C+FRPY CPYAG ECSVTGDIP LV+HLK+DH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 342 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 401
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 402 LEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIW 459
           LEVG +GRKL WQG+PRSIRDSHRKVRDS DGLII RNLAL+ SG + QELKL+V GRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of Spg000749 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.7e-130
Identity = 218/299 (72.91%), Postives = 250/299 (83.61%), Query Frame = 0


Query: 162 SFCKEVTELRLTSIDSEAATSRSE--SKGVSFRKASTGGFVRNRNDVHELLDCTVCMNLM 221
           S  KE  E   T +D E   ++ E  SK       S G F  + N V+ELL+C VC NLM
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF-HSSNGVYELLECPVCTNLM 65

Query: 222 HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGCID 281
           +PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC D
Sbjct: 66  YPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQD 125

Query: 282 IFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHRYV 341
           IFPYYSKLKHE++C+FR Y+CPYAG ECSVTGDIP LV HLK+DH+VDMHDGC+F+HRYV
Sbjct: 126 IFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYV 185

Query: 342 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLE 401
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLE
Sbjct: 186 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 245

Query: 402 VGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIWR 459
           VG + RKL WQG+PRSIRDSHRKVRDS DGLII RNLAL+ SG++ +ELKL+V GRIW+
Sbjct: 246 VGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of Spg000749 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 7.7e-125
Identity = 198/265 (74.72%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 194 ASTGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 253
           A+  G +     VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 254 IRCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDI 313
           IRCLALEKVAESLELPC + +LGC +IFPYYSKLKHE  C FRPY+CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 314 PYLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 373
           P+LV HL++DH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 374 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 433
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 434 RNLALFCSGNEGQELKLKVAGRIWR 459
           RN+ALF SG + +ELKL+V GRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of Spg000749 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.0e-124
Identity = 195/255 (76.47%), Postives = 227/255 (89.02%), Query Frame = 0


Query: 204 NDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVA 263
           + V ELL+C VC++ M+PPI+QC+NGHTLCS CK RV N CPTCRHELGNIRCLALEKVA
Sbjct: 45  SSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 104

Query: 264 ESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKED 323
            SLELPC Y+N GC+ I+PYY KLKHE  C++RPY+CPYAG EC+V GDIPYLV HLK+D
Sbjct: 105 ASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDD 164

Query: 324 HEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLR 383
           H+VDMH+GC+F+HRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAF+LG APVY+AFLR
Sbjct: 165 HKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 224

Query: 384 FMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGN 443
           FMGDD +AK +SYSLEVGG GRK+IWQG+PRSIRDSHRKVRDS+DGLIIQRN+ALF SG 
Sbjct: 225 FMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGG 284

Query: 444 EGQELKLKVAGRIWR 459
           E +ELKL+V GRIW+
Sbjct: 285 ERKELKLRVTGRIWK 299

[Close]

BLAST of Spg000749 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 439.5 bits (1129), Expect = 4.7e-122
Identity = 200/288 (69.44%), Postives = 232/288 (80.56%), Query Frame = 0


Query: 174 SIDSEAATSRSESKGVSFRKASTGG---FVRNRNDVHELLDCTVCMNLMHPPIYQCANGH 233
           SIDS         K       S GG    +     V+ELL+C VC N M+PPI+QC NGH
Sbjct: 5   SIDSVIDDDEIHQKHQFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNGH 64

Query: 234 TLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHE 293
           TLCS+CK+RV N CPTCR ELG+IRCLALEKVAESLELPC Y NLGC+ IFPYYSKLKHE
Sbjct: 65  TLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKHE 124

Query: 294 KNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENAT 353
             C FRPY+CPYAG EC+  GDI +LV HL++DH+VDMH GC+F+HRYVKSNP+EVENAT
Sbjct: 125 SQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENAT 184

Query: 354 WMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQ 413
           WMLTVF CFG+ FCLHFEAF+LG APVYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+
Sbjct: 185 WMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWE 244

Query: 414 GMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIWR 459
           G PRS+RDSHRKVRDSHDGLIIQRN+ALF SG + +ELKL+V GRIW+
Sbjct: 245 GTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWK 292

[Close]

BLAST of Spg000749 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.9e-181
Identity = 300/326 (92.02%), Postives = 314/326 (96.32%), Query Frame = 0


Query: 136 MST-VDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKA 195
           MST +++RE GMYQQQKSREEHASSAASFCKEVTEL LTSIDS+ ATSR E KGV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 196 STGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 255
           STGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 256 RCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIP 315
           RCLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 316 YLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 375
            LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 376 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 435
           APVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 436 NLALFCSGNEGQELKLKVAGRIWRVR 461
            LALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Spg000749 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.3e-180
Identity = 300/326 (92.02%), Postives = 313/326 (96.01%), Query Frame = 0


Query: 136 MST-VDYRENGMYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKA 195
           MST +++RE GMYQQ KSREEHASSAASFCKEVTELRLTSIDS+ ATSR E KGV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 196 STGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 255
           STGGF R+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 256 RCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIP 315
           RCLALEKVAESLELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 316 YLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 375
            LVMHLK+DHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 376 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 435
           APVYMAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 436 NLALFCSGNEGQELKLKVAGRIWRVR 461
            LALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Spg000749 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 8.1e-178
Identity = 294/315 (93.33%), Postives = 305/315 (96.83%), Query Frame = 0


Query: 146 MYQQQKSREEHASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKASTGGFVRNRND 205
           MYQQQKSREEHASSAASFCKEVTEL LTSIDS+ ATSR E KGV+FRKASTGGF R+RND
Sbjct: 1   MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 60

Query: 206 VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 265
           VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 120

Query: 266 LELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHE 325
           LELPC+Y+NLGCID+FPYYSKLKHEKNCK+RPYNCPYAGGEC VTGDIP LVMHLK+DHE
Sbjct: 121 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 180

Query: 326 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 385
           VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 181 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240

Query: 386 GDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEG 445
           GDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR LALFCSGNEG
Sbjct: 241 GDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 300

Query: 446 QELKLKVAGRIWRVR 461
            ELKLKV GRIWRVR
Sbjct: 301 MELKLKVTGRIWRVR 315

[Close]

BLAST of Spg000749 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 1.3e-170
Identity = 287/326 (88.04%), Postives = 304/326 (93.25%), Query Frame = 0


Query: 136 MSTVDYRENGMYQQQKSREEH-ASSAASFCKEVTELRLTSIDSEAATSRSESKGVSFRKA 195
           MS V++RE  MYQQ+K REEH +SS ASFCKEVTELRLTSIDS+AATSR E K VS RK+
Sbjct: 1   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 60

Query: 196 STGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 255
           ST GF RN N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNI
Sbjct: 61  ST-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNI 120

Query: 256 RCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIP 315
           RCL LEKVAESLELPC+Y+NLGCID  PYYSKLKHEKNCK+RPYNCPYAGGECSVTGDIP
Sbjct: 121 RCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 180

Query: 316 YLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 375
           YLVMHLK+DHEVDMH+GCSFSHRYVKSNP+EVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 YLVMHLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 376 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 435
           APVYMAFLRFMGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R
Sbjct: 241 APVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHR 300

Query: 436 NLALFCSGNEGQELKLKVAGRIWRVR 461
           NLALFCSGN GQELKLKV GRIWRVR
Sbjct: 301 NLALFCSGNHGQELKLKVVGRIWRVR 325

[Close]

BLAST of Spg000749 vs. ExPASy TrEMBL
Match: A0A7N2KLK5 (RING-type E3 ubiquitin transferase OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.8e-140
Identity = 237/301 (78.74%), Postives = 261/301 (86.71%), Query Frame = 0


Query: 163 FCKEVTELRLTSIDSEAATSRSESKGVSFRKASTG-----GFVRNRNDVHELLDCTVCMN 222
           FCKEVTE   TS+DS+ ATS +E    ++RKA  G     G   N NDVH+LL+C VCMN
Sbjct: 2   FCKEVTESCTTSMDSDLATSSAELGSSNYRKADPGLSGNPGRPSN-NDVHDLLECPVCMN 61

Query: 223 LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGC 282
           LM+PPIYQC +GHTLCS+CK RV NCCP CRHELGNIRCLALEKVAESLELPC Y+ LGC
Sbjct: 62  LMYPPIYQCPSGHTLCSNCKVRVHNCCPACRHELGNIRCLALEKVAESLELPCRYQILGC 121

Query: 283 IDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHR 342
            DIFPYYSKLKHEKNCK+RPYNCPYAG +CSVTGDIP+LVMHLK DH+VDMHDGCSFSHR
Sbjct: 122 HDIFPYYSKLKHEKNCKYRPYNCPYAGADCSVTGDIPFLVMHLKNDHKVDMHDGCSFSHR 181

Query: 343 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 402
           YVKSNPQEV NATWMLTVFNCFGRQFCLHFEAF LG APVYMAFLRFMGD+D+AK+FSYS
Sbjct: 182 YVKSNPQEVGNATWMLTVFNCFGRQFCLHFEAFLLGLAPVYMAFLRFMGDEDEAKEFSYS 241

Query: 403 LEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIW 459
           LEVG NGRKL WQG+PRSIRDSH+KVRDS DGLIIQRNLAL  SG + QELKLK++GRIW
Sbjct: 242 LEVGRNGRKLTWQGIPRSIRDSHKKVRDSQDGLIIQRNLALLFSGGDKQELKLKISGRIW 301

[Close]

BLAST of Spg000749 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 475.7 bits (1223), Expect = 4.2e-134
Identity = 219/301 (72.76%), Postives = 252/301 (83.72%), Query Frame = 0


Query: 162 SFCKEVTELRLTSIDSEAATSRSESKGVSFRKASTGGF----VRNRNDVHELLDCTVCMN 221
           S  KEV E   T +D E  T++ E       K  + G     + + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 222 LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGC 281
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 282 IDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHR 341
            DIFPYYSKLKHE++C+FRPY CPYAG ECSVTGDIP LV+HLK+DH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 342 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 401
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 402 LEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIW 459
           LEVG +GRKL WQG+PRSIRDSHRKVRDS DGLII RNLAL+ SG + QELKL+V GRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of Spg000749 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 466.8 bits (1200), Expect = 1.9e-131
Identity = 218/299 (72.91%), Postives = 250/299 (83.61%), Query Frame = 0


Query: 162 SFCKEVTELRLTSIDSEAATSRSE--SKGVSFRKASTGGFVRNRNDVHELLDCTVCMNLM 221
           S  KE  E   T +D E   ++ E  SK       S G F  + N V+ELL+C VC NLM
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF-HSSNGVYELLECPVCTNLM 65

Query: 222 HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCMYENLGCID 281
           +PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC D
Sbjct: 66  YPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQD 125

Query: 282 IFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHEVDMHDGCSFSHRYV 341
           IFPYYSKLKHE++C+FR Y+CPYAG ECSVTGDIP LV HLK+DH+VDMHDGC+F+HRYV
Sbjct: 126 IFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYV 185

Query: 342 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLE 401
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLE
Sbjct: 186 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 245

Query: 402 VGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEGQELKLKVAGRIWR 459
           VG + RKL WQG+PRSIRDSHRKVRDS DGLII RNLAL+ SG++ +ELKL+V GRIW+
Sbjct: 246 VGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of Spg000749 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 448.7 bits (1153), Expect = 5.5e-126
Identity = 198/265 (74.72%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 194 ASTGGFVRNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 253
           A+  G +     VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 254 IRCLALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDI 313
           IRCLALEKVAESLELPC + +LGC +IFPYYSKLKHE  C FRPY+CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 314 PYLVMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 373
           P+LV HL++DH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 374 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 433
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 434 RNLALFCSGNEGQELKLKVAGRIWR 459
           RN+ALF SG + +ELKL+V GRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of Spg000749 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 431.0 bits (1107), Expect = 1.2e-120
Identity = 189/253 (74.70%), Postives = 222/253 (87.75%), Query Frame = 0


Query: 206 VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 265
           V+ELL+C VC   M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 266 LELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYLVMHLKEDHE 325
           LELPC + NLGC +IFPYYSKLKHE  C FRPY+CPYAG EC + GDIP+LV HL++DH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 326 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 385
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 386 GDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNLALFCSGNEG 445
           GD++DA+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQRN+ALF SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 446 QELKLKVAGRIWR 459
           +ELKL+V G+IW+
Sbjct: 298 KELKLRVTGKIWK 310

[Close]

BLAST of Spg000749 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 154/202 (76.24%), Postives = 179/202 (88.61%), Query Frame = 0


Query: 257 LALEKVAESLELPCMYENLGCIDIFPYYSKLKHEKNCKFRPYNCPYAGGECSVTGDIPYL 316
           +ALEKVAESLELPC Y NLGC+ IFPYYSKLKHE  C FRPY+CPYAG EC+  GDI +L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 317 VMHLKEDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 376
           V HL++DH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 377 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRNL 436
           VYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQRN+
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 437 ALFCSGNEGQELKLKVAGRIWR 459
           ALF SG + +ELKL+V GRIW+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWK 216

The following BLAST results are available for this feature:

BLAST of Spg000749 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900383.1	1.6e-183	92.90	E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida]	[more]
XP_038900384.1	1.6e-183	92.90	E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890...	[more]
XP_038900382.1	1.6e-183	92.90	E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida]	[more]
XP_008449780.1	1.6e-180	92.02	PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo]	[more]
XP_031740164.1	2.8e-180	92.02	E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetic...	[more]

BLAST of Spg000749 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2P4	5.9e-133	72.76	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]
P93748	2.7e-130	72.91	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q84JL3	7.7e-125	74.72	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]
Q10L91	1.0e-124	76.47	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
Q8S3N1	4.7e-122	69.44	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=...	[more]

BLAST of Spg000749 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BMT4	7.9e-181	92.02	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ...	[more]
A0A0A0KWV9	1.3e-180	92.02	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE...	[more]
A0A5D3DDZ5	8.1e-178	93.33	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1DI06	1.3e-170	88.04	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126...	[more]
A0A7N2KLK5	1.8e-140	78.74	RING-type E3 ubiquitin transferase OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of Spg000749 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58040.1	4.2e-134	72.76	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	1.9e-131	72.91	Protein with RING/U-box and TRAF-like domains	[more]
AT3G61790.1	5.5e-126	74.72	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	1.2e-120	74.70	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	1.1e-97	76.24	TRAF-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 254..452 e-value: 9.7E-79 score: 264.0
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 265..320 e-value: 1.0E-15 score: 59.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 146..264 e-value: 2.6E-6 score: 29.0
IPR008974	TRAF-like	GENE3D	2.60.210.10	Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A	coord: 321..453 e-value: 2.8E-35 score: 122.9
None	No IPR available	PANTHER	PTHR10315:SF123	E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKE	coord: 161..458
None	No IPR available	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 161..458
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 210..248 e-value: 6.11156E-14 score: 63.843
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 265..456
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 203..254
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 265..325 score: 13.741816
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 212..248 score: 9.214112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000749.1	Spg000749.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding