Spg000716 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000716
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAPO protein 4, mitochondrial-like
Locationscaffold8: 41185762 .. 41188831 (+)
RNA-Seq ExpressionSpg000716
SyntenySpg000716
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCGCCGCCGCCTCTCTCCCATTCACAGCTACCGCCTCCGTCGTCTTTCTTCCGATCGTGCCGCCACCACCGTCTTCTTTCTTCCTTTCATGCCACTGCAGCCTCCCTCGATTTTTTACTCCCTTGTTACTGGTATTCTCCCAATCTCCGTCTGTCATTCTTCTCACATGAAAAACACGAATTAGAACTTGTCTAGTGAAAGAATGGAGGAGTTGTTTGATTTTTACTTTTTAGCAACGGAAAAAAATAGCCTTGCTGCACATTAGGGTGTTTGGCTGTTGAATCAGTCATCCAATTCTGTTTTGATTCGTAGATCGATTGACCGAAAACTTCAACCATATATTTGTATGTCTGTAGATTTTAGTTGGGAGAAGACAATGAGTTTCTTTTTTTTTCGTCGCTCCTCTCTGTTAGTCGGTTCATCTTGTCTCTGAAAGCTCTCTGCCGTTTGTCTACTCTCGAAATCTCATCATTTGTTCCTTAAACCCTCTTTCTTACTCGCTGTCTCGAGCACTCTCTTCTCCGTTGAATTCGAAGGCTCACGCTACGCCCTCCTGGCTCATGCGATTGGATTGAGACCTTCATTTATACCTTCAAATTTGTGGCTTCTCCTTCTGCTACGATTTGGGACATGTTAGGGCTTGCACCATGAAAACTATGGCTTTCATGGCTCTTAGAAGGAAGTTGAGTGAGAATGTTGTGCACGAATTCATGCTGCAGCGGTGCTACAGCTCTAAAGTCGACCTTAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCGAAAAACTATCCCATAAAGGGTATGATTCCTGTGGCGCAGCAAGTCCTTGAAGCTAGAACAATGCTTATGCATGGAGTTTCTACTCTTCTCAAAGCATTTCCAGTTGTCTCTTGCAAGTCAGTGTCCTGATAACAAAGTCTCTTTAATTTCTCATCTTTGTGTAATGATTTTACTCTGTTTCGATTTGTTTCCCTTACTTTTTACATAGTTTTTTTTAATCTAAATTGCATATTCCTTGAAGTAATTTGTCATTACAGTAGTACCATTTTGTTAGAAATGCGGAGTGACCATGTTTTTTTAGGTCTCCTTTGATAACCATTTGGTTTTTGGTTTTTGGTTTTTGAAAATTATGTTTGTTTTCTCTCCATTTTCCTACCATGATTTTCATCTTTGTCAAGGGAACATTGGAATTCCTAGCCAAATTCCAAAAACAAAAACAAGTTTTTGAAAACGACTTTTTTTAGTTTTTAAAATTTGGCTTGGTTTTTGAAAACTTGGAAATAATGTATTAAGAAAACAAAGAAATTTGTAGGTGGAAGTAGTGTTTATAGGTATAATTTTCAAAAACCAAAAACCAAATGGTTATCAAACGGGGCCTTAGTAATATGTTTCCTGACAAGCAGCTTATATAAGAAATTTCCTGCAAATGAAGTGAGGTCAATGACCAACAATTTACATTATCTAGTTCTTTATGTACATAGGACCGTTTCTAAAGTCTGTTCTTTCTGAAATTTTAATTTAACCATGAGGTGTTAATATGAATGCATGTTTGTGAATGTTGCTTCTAAAACTCCTAGACTTGTGTATGTCTGTTCTTTCTGTAGTTTGAATTCAACAACCTCGGCTGTTAACATGAATACATGTTTTTGAATGTTGCTTCTAAAAACTCCTAGACTCATTGCATAAGTCTGTTCTTTCTGTAGTTTGAATTCAACAATCTCAGCTGTTAACATGAATACATGTTGTGAGTTCTGCTTCTAAAGCTTTTAGGATCATTGTATAATTTTTGTAGATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTAATACGAAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATAAGGTTCATGAATGGATCAAAGGTGATTTGAATGATATCCTTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCTGTTGTCGAGCTATGCTGGCAGGCTGGAGCTAATCCAAATGATGAAAATCTTTCTTCAAACACTTGGAATTCAGATGGTAATGGCTTAGGCAGGGATGAACCTTTGTCAAGTCATGAGCTGAGGCTTTTAGCAAATACAACTATCAGGGCATGGGAAACACTCAGAACCGGAGTTCAGAAACTGTTAATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGGCACATGGCTAGACTCTGTGGAGTTTTTAAATATGAGAGTTGGCGAGGATCTCATCGCTGGGAGAAGGCAGACGTAGATGATTTAGTCCCTCCAAAGATTGTATGGCATCGACGACCACAAGATCCTCCTGTGCTCGTCGATGAAGGGAGGGATTATTATGGACACACGCCAGCAGTGATAGCTCTTTGCACACAGGCTGGTGCCATTGCACCATCTAAGTATTACTGTATGATGAAAGTTCAAGGCTTATCACATCAGAGTCAACTCAAGCTCTGAAATCTTCTTCTGTTTAAATTCATGTGGACTTGCCCAGAACACTCCACTCGTTCACGACTTCAGGTGACCGTGGCTAATTTCCTATATATTTTCTTTCTTCCTACTCTGTCTGTTTGGTTGATTTTTTAGACATAGAGAAAGAGAAACACGATACATCATCAAGCATGACAACTTAGGAAAGGTTACTGAAAAGTTTTGTAGCTCACAAAGTTGAATAATATTGAACCTGAGATATCAATAGTTGAAACTAACTTCACAAAGAATGTCTTATAGAATCGCCTCCATTCTTTCCATTAGAACAACAAGTAATGGGAGGCACTTTATGGAAGAATTGTATGTAAATGGGAAGAATTGTATGTAAATGCAAGATATATATCCTTGCTTGGAAATTGTTACTTTTTTCACCCACTTATAAGTATCCTGTGTTTGAAGTAATTTTTGTGAGTTTTAAGGAACTTTTGCATGAAGCTATTCAACATCGCGACAACGTTATAAATATGCATCAGGTCGATCAACCATTCACTATTTGAA

mRNA sequence

ATGTCGTCGCCGCCGCCTCTCTCCCATTCACAGCTACCGCCTCCGTCGTCTTTCTTCCGATCGTGCCGCCACCACCGTCTTCTTTCTTCCTTTCATGCCACTGCAGCCTCCCTCGATTTTTTACTCCCTTGTTACTGGGCTTGCACCATGAAAACTATGGCTTTCATGGCTCTTAGAAGGAAGTTGAGTGAGAATGTTGTGCACGAATTCATGCTGCAGCGGTGCTACAGCTCTAAAGTCGACCTTAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCGAAAAACTATCCCATAAAGGGTATGATTCCTGTGGCGCAGCAAGTCCTTGAAGCTAGAACAATGCTTATGCATGGAGTTTCTACTCTTCTCAAAGCATTTCCAGTTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTAATACGAAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATAAGGTTCATGAATGGATCAAAGGTGATTTGAATGATATCCTTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCTGTTGTCGAGCTATGCTGGCAGGCTGGAGCTAATCCAAATGATGAAAATCTTTCTTCAAACACTTGGAATTCAGATGGTAATGGCTTAGGCAGGGATGAACCTTTGTCAAGTCATGAGCTGAGGCTTTTAGCAAATACAACTATCAGGGCATGGGAAACACTCAGAACCGGAGTTCAGAAACTGTTAATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGGCACATGGCTAGACTCTGTGGAGTTTTTAAATATGAGAGTTGGCGAGGATCTCATCGCTGGGAGAAGGCAGACGTAGATGATTTAGTCCCTCCAAAGATTGTATGGCATCGACGACCACAAGATCCTCCTGTGCTCGTCGATGAAGGGAGGGATTATTATGGACACACGCCAGCAGTGATAGCTCTTTGCACACAGGCTGGTGCCATTGCACCATCTAAGTATTACTGTATGATGAAAGTTCAAGGCTTATCACATCAGAGTCAACTCAAGCTCTGA

Coding sequence (CDS)

ATGTCGTCGCCGCCGCCTCTCTCCCATTCACAGCTACCGCCTCCGTCGTCTTTCTTCCGATCGTGCCGCCACCACCGTCTTCTTTCTTCCTTTCATGCCACTGCAGCCTCCCTCGATTTTTTACTCCCTTGTTACTGGGCTTGCACCATGAAAACTATGGCTTTCATGGCTCTTAGAAGGAAGTTGAGTGAGAATGTTGTGCACGAATTCATGCTGCAGCGGTGCTACAGCTCTAAAGTCGACCTTAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCGAAAAACTATCCCATAAAGGGTATGATTCCTGTGGCGCAGCAAGTCCTTGAAGCTAGAACAATGCTTATGCATGGAGTTTCTACTCTTCTCAAAGCATTTCCAGTTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTAATACGAAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATAAGGTTCATGAATGGATCAAAGGTGATTTGAATGATATCCTTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCTGTTGTCGAGCTATGCTGGCAGGCTGGAGCTAATCCAAATGATGAAAATCTTTCTTCAAACACTTGGAATTCAGATGGTAATGGCTTAGGCAGGGATGAACCTTTGTCAAGTCATGAGCTGAGGCTTTTAGCAAATACAACTATCAGGGCATGGGAAACACTCAGAACCGGAGTTCAGAAACTGTTAATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGGCACATGGCTAGACTCTGTGGAGTTTTTAAATATGAGAGTTGGCGAGGATCTCATCGCTGGGAGAAGGCAGACGTAGATGATTTAGTCCCTCCAAAGATTGTATGGCATCGACGACCACAAGATCCTCCTGTGCTCGTCGATGAAGGGAGGGATTATTATGGACACACGCCAGCAGTGATAGCTCTTTGCACACAGGCTGGTGCCATTGCACCATCTAAGTATTACTGTATGATGAAAGTTCAAGGCTTATCACATCAGAGTCAACTCAAGCTCTGA

Protein sequence

MSSPPPLSHSQLPPPSSFFRSCRHHRLLSSFHATAASLDFLLPCYWACTMKTMAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGNGLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQAGAIAPSKYYCMMKVQGLSHQSQLKL
Homology
BLAST of Spg000716 vs. NCBI nr
Match: XP_023532078.1 (APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.5 bits (1573), Expect = 9.6e-171
Identity = 290/327 (88.69%), Postives = 306/327 (93.58%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR  L+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYK GAKN+VHEWI+GDL
Sbjct: 61  LEARAKLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNS--D 232
           NDILVPVE+FHL HMFQDVIKHDERF+F+RVPAVVELCWQAGANPNDENLSS+TWNS  D
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFDRVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 233 GNGLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMA 292
           GNG GRDE LSSHEL +LANTTIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGH A
Sbjct: 181 GNGNGRDEHLSSHELMVLANTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKA 240

Query: 293 RLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALC 352
           RLCGVFKYESWRGSH WEKADVDDLVPPK VWHRR QDPPVLVDEGRDYYGH PAV+ALC
Sbjct: 241 RLCGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALC 300

Query: 353 TQAGAIAPSKYYCMMKVQGLSHQSQLK 378
            QAGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 LQAGAIAPSKYHCMMKVQGLSHHSQLK 327

BLAST of Spg000716 vs. NCBI nr
Match: XP_022971049.1 (APO protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 289/325 (88.92%), Postives = 306/325 (94.15%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR ML+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYKRGAKN+VHEWI+GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGN 232
           NDILVPVE+FHL HMFQDVIKHDERF+FERVPAVVELCWQAGANPNDENLSS+TWNSDG+
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 233 GLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARL 292
             GRDE LSSHEL +LAN+TIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGH ARL
Sbjct: 181 --GRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 293 CGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQ 352
           CGVFKYESWRGSH WEK DVDDLVPPK VWHRR QDPPVLVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 353 AGAIAPSKYYCMMKVQGLSHQSQLK 378
           AGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of Spg000716 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 608.6 bits (1568), Expect = 3.6e-170
Identity = 287/330 (86.97%), Postives = 310/330 (93.94%), Query Frame = 0

Query: 50  MKTMAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVA 109
           MKTMAFMAL+RK  E VV EF+L RCYSSKV+LKKLRPMILKRIQNR KNYPI+GM PVA
Sbjct: 1   MKTMAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVA 60

Query: 110 QQVLEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIK 169
           QQVLEARTML+HGVSTLLK+FPV+SCKFCPEVYVGEKGHLIRSCGGYKRGAKN+VHEWI+
Sbjct: 61  QQVLEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIR 120

Query: 170 GDLNDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNS 229
           GDLNDI+VPVE+FHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDEN SS+T NS
Sbjct: 121 GDLNDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNS 180

Query: 230 -DGNGLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 289
            +G G GRD+PLS HE+ LLA  TIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH
Sbjct: 181 GEGGGSGRDKPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 240

Query: 290 MARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIA 349
            ARLCGVFKYE WRGSH WEKAD+DDLVPPK+VWHRRPQDPPVL+DEGRDYYGHTPAV+A
Sbjct: 241 KARLCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGHTPAVLA 300

Query: 350 LCTQAGAIAPSKYYCMMKVQGLSHQSQLKL 379
           LCTQAGA+APSKY+CMMKVQG+S +SQLKL
Sbjct: 301 LCTQAGALAPSKYHCMMKVQGISPRSQLKL 330

BLAST of Spg000716 vs. NCBI nr
Match: XP_022947979.1 (APO protein 4, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 608.2 bits (1567), Expect = 4.8e-170
Identity = 288/325 (88.62%), Postives = 304/325 (93.54%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR ML+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYK GAKN+VHEW +GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWFRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGN 232
           NDILVPVE+FHLHHMFQDVIKHDERF+FERVPAVVELCWQAGANPNDE+LSS TWNSDGN
Sbjct: 121 NDILVPVESFHLHHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDEHLSSGTWNSDGN 180

Query: 233 GLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARL 292
             GRDE LSSHEL +LA+TTIRAWET+RTGV KLLMVYP KVCK+CSEVHVGPSGH ARL
Sbjct: 181 --GRDEHLSSHELMVLASTTIRAWETVRTGVHKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 293 CGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQ 352
           CGVFKYESWRGSH WEKADVDDLVPPK VWHRR QDPPVLVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCLQ 300

Query: 353 AGAIAPSKYYCMMKVQGLSHQSQLK 378
           AGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of Spg000716 vs. NCBI nr
Match: KAG6604849.1 (APO protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 604.7 bits (1558), Expect = 5.3e-169
Identity = 287/325 (88.31%), Postives = 305/325 (93.85%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR ML+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYK GAKN+VHEWI+GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGN 232
           N ILVPVE+FHLHHMFQDVIKHDERF+FERVPAVVELCWQAGANPNDE+LSS+TWNSDG+
Sbjct: 121 NHILVPVESFHLHHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDEHLSSSTWNSDGD 180

Query: 233 GLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARL 292
             GRDE LSSHEL +LA+TTIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGH ARL
Sbjct: 181 --GRDEHLSSHELMVLASTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 293 CGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQ 352
           CGVFKYESWRGSH WEKADVDDLVPPK VWHRR QDPPVLVDEGR YYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRHYYGHAPAVLALCLQ 300

Query: 353 AGAIAPSKYYCMMKVQGLSHQSQLK 378
           AGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of Spg000716 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 380.6 bits (976), Expect = 2.1e-104
Identity = 174/292 (59.59%), Postives = 226/292 (77.40%), Query Frame = 0

Query: 81  DLKKLRPMILKRIQNRAKNYPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPE 140
           DL+KLRPMILKRI+NRAK+YP+K ++PVA+++L AR  L+  ++ LLK FPV++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 141 VYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNF 200
           V+VG++GHLI +C  Y R   N++HEW+ G +NDILVPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 201 ERVPAVVELCWQAGANPNDENLSSNTWNSDGNGLGRD-EPLSSHELRLLANTTIRAWETL 260
           +RVPA++ELC QAGA   +E L  +  + +      D   L + +L+ +    + AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 261 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPK 320
           R GV+KLL+VYP+KVCK C EVHVGPSGH ARLCGVFKYESWRG+H WEKA V+DLVP K
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 321 IVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQAGAIAPSKYYCMMKVQGLS 372
           +VWHRRPQDP VLVDEGR YYGH PA+++LC+  GAI P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of Spg000716 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 6.2e-56
Identity = 120/328 (36.59%), Postives = 172/328 (52.44%), Query Frame = 0

Query: 72  LQRCYSSKVDLKKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAF 131
           L+R    +V   K +P   KR     KN   +K ++P+A +V  AR  L++ +  L+K  
Sbjct: 102 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 161

Query: 132 PVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQD 191
            V +C +C E++VG  GH  +SC G     +  +HEW    + D++VP+EA+HL      
Sbjct: 162 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 221

Query: 192 VIKHDERFNFERVPAVVELCWQAGA-----------NPNDENLSSNTWNSDGNGLGRDE- 251
            I+HDERF+  RVPAVVELC Q G             P      S   ++D   L   E 
Sbjct: 222 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 281

Query: 252 ------------------PLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSE 311
                             P S  E   LA  T++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 282 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 341

Query: 312 VHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYY 369
           VHVGP+GH A+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R +Y
Sbjct: 342 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 401

BLAST of Spg000716 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.7e-53
Identity = 116/328 (35.37%), Postives = 170/328 (51.83%), Query Frame = 0

Query: 83  KKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPEV 142
           KKL  M +++  +  KN   +  ++PVA QV++   +L+ G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 143 YVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNFE 202
           +V   GH IR C G     +   H W+KG +ND+L+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 203 RVPAVVELCWQAGAN---------------------------------PNDENLSSNTWN 262
           R+PA+VELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 263 SDGNGL-GRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 322
            D  G+  R  P +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 323 HMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVI 376
           H  +LCG FK++   G H W+ A VD++ PP  VWH R      L    R +YG  PA++
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of Spg000716 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 9.4e-44
Identity = 98/291 (33.68%), Postives = 156/291 (53.61%), Query Frame = 0

Query: 105 MIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKV 164
           ++ VA  V   R ML+ G+S ++   PV  C+ C EV++G++GH IR+C G   G+++  
Sbjct: 98  LVDVAHCVHRCRNMLLSGLSKIIHHVPVHRCRLCAEVHIGKQGHEIRTCTGPGSGSRSAT 157

Query: 165 HEWIKGDLNDILVPVEAFHLH-HMFQDVIKHDERFNFERVPAVVELCWQAGANPN---DE 224
           H W +G ++D+++  + FHL+    +  + HDERF   ++ AV+ELC QAG +      +
Sbjct: 158 HVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAVLELCIQAGVDLEKFPSK 217

Query: 225 NLSSNTWN-----------SDGNG----------LGRDEPLSSHE--LRLLANTTIRAWE 284
             S   ++           +DGN           +  D+     +  L+ L+  T+ +W 
Sbjct: 218 RRSKPVYSIEGRIVDFEDVNDGNSELAVTSTTTLIQEDDRCKEEKKSLKELSFETMESWF 277

Query: 285 TLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVP 344
            +  GV+KL+  Y    C YC E+ VGP GH  R+C   K++   G H W++A +DD+V 
Sbjct: 278 EMVLGVRKLMERYRVWTCGYCPEIQVGPKGHKVRMCKATKHQMRDGMHAWQEATIDDVVG 337

Query: 345 PKIVWH-RRPQDPPVLVDEGRDYYGHTPAVIALCTQAGAIAPSKYYCMMKV 368
           P  VWH R P D  VL +  + +YG  PAVI +C Q GA  P +Y  MM++
Sbjct: 338 PTYVWHVRDPTDGSVLDNSLKRFYGKAPAVIEMCVQGGAPVPDQYNSMMRL 388

BLAST of Spg000716 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.9e-171
Identity = 289/325 (88.92%), Postives = 306/325 (94.15%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR ML+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYKRGAKN+VHEWI+GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGN 232
           NDILVPVE+FHL HMFQDVIKHDERF+FERVPAVVELCWQAGANPNDENLSS+TWNSDG+
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 233 GLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARL 292
             GRDE LSSHEL +LAN+TIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGH ARL
Sbjct: 181 --GRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 293 CGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQ 352
           CGVFKYESWRGSH WEK DVDDLVPPK VWHRR QDPPVLVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 353 AGAIAPSKYYCMMKVQGLSHQSQLK 378
           AGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of Spg000716 vs. ExPASy TrEMBL
Match: A0A6J1G8H9 (APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.3e-170
Identity = 288/325 (88.62%), Postives = 304/325 (93.54%), Query Frame = 0

Query: 53  MAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQV 112
           MAFMALRRKL ENVV EFMLQRCYSSKVDLKKLRPMILKR+QNRA NYPIKGMIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 113 LEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDL 172
           LEAR ML+HGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYK GAKN+VHEW +GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWFRGDL 120

Query: 173 NDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGN 232
           NDILVPVE+FHLHHMFQDVIKHDERF+FERVPAVVELCWQAGANPNDE+LSS TWNSDGN
Sbjct: 121 NDILVPVESFHLHHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDEHLSSGTWNSDGN 180

Query: 233 GLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARL 292
             GRDE LSSHEL +LA+TTIRAWET+RTGV KLLMVYP KVCK+CSEVHVGPSGH ARL
Sbjct: 181 --GRDEHLSSHELMVLASTTIRAWETVRTGVHKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 293 CGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQ 352
           CGVFKYESWRGSH WEKADVDDLVPPK VWHRR QDPPVLVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCLQ 300

Query: 353 AGAIAPSKYYCMMKVQGLSHQSQLK 378
           AGAIAPSKY+CMMKVQGLSH SQLK
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of Spg000716 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.2e-164
Identity = 279/330 (84.55%), Postives = 304/330 (92.12%), Query Frame = 0

Query: 50  MKTMAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVA 109
           MKTMAFMA+RRK  +NVV EFMLQRCYSSKV+LKKLRPMILKRIQ+RAKNYPIKGM PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 110 QQVLEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIK 169
           QQVLEAR ML+HGVSTLLK+FPV+SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VH+WI+
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 170 GDLNDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNS 229
           GDL DI+VPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAGANP+D+NL+S+T NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 230 -DGNGLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 289
            +G G G DEPLS HE+ LLA  TIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 290 MARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIA 349
            ARLCGVF YESWRGSH WEKADVDDLVPPKIVWHRR QDPPVLVD+G+DYYGH PAV+A
Sbjct: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300

Query: 350 LCTQAGAIAPSKYYCMMKVQGLSHQSQLKL 379
           LCTQAG IAP KY+CMMKVQGLS + +L+L
Sbjct: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of Spg000716 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 6.1e-163
Identity = 277/328 (84.45%), Postives = 301/328 (91.77%), Query Frame = 0

Query: 50  MKTMAFMALRRKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVA 109
           MKTMAFMA+RRK  +NVV EFMLQRCYSSKV+LKKLRPMILKRIQ+RAKNYPIKGM PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 110 QQVLEARTMLMHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIK 169
           QQVLEAR ML+HGVSTLLK+FPV+SCK+CPEVYVG +GHLIRSCGGYKRGAKN+VH+WI+
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 170 GDLNDILVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNS 229
           GDLNDI+VPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+S+T NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 230 -DGNGLGRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 289
            +G G G DEP S HE+ LLA  TIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 290 MARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIA 349
            ARLCGVFKYESWRGSH WEKADVDDLVPPKIVWHRR QDPPVLVD+GRDYYGH PAV+A
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 350 LCTQAGAIAPSKYYCMMKVQGLSHQSQL 377
           LCTQAGAIAP KY+CMMKVQGLS +  L
Sbjct: 301 LCTQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of Spg000716 vs. ExPASy TrEMBL
Match: A0A6J1CIR9 (APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.0e-162
Identity = 274/319 (85.89%), Postives = 293/319 (91.85%), Query Frame = 0

Query: 60  RKLSENVVHEFMLQRCYSSKVDLKKLRPMILKRIQNRAKNYPIKGMIPVAQQVLEARTML 119
           RKL +NVV EFML RCYSSKVDLKKLRPMI KRI+NRAK YPI  MIPVAQQVLEAR +L
Sbjct: 22  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 81

Query: 120 MHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPV 179
           +HGVSTLLKAFP+VSCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VHEWI GDLND+LVPV
Sbjct: 82  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 141

Query: 180 EAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSNTWNSDGNGLGRDEP 239
           EAFHLH MFQDVIKHDERFNFERVPAV ELCWQAGANPNDENLSS+T NSDG G GRDE 
Sbjct: 142 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 201

Query: 240 LSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARLCGVFKYE 299
           LSSHEL  LAN T++AWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH ARLCGVFKYE
Sbjct: 202 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 261

Query: 300 SWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQAGAIAPS 359
           SWRGSH WEKA+VDDLVPP IVWHRRPQDPP+LVDEGRDYYGH PAV+ALCTQAG +APS
Sbjct: 262 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 321

Query: 360 KYYCMMKVQGLSHQSQLKL 379
           KY+CMMK+QGLS ++Q  L
Sbjct: 322 KYHCMMKIQGLSPRTQQNL 340

BLAST of Spg000716 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-105
Identity = 174/292 (59.59%), Postives = 226/292 (77.40%), Query Frame = 0

Query: 81  DLKKLRPMILKRIQNRAKNYPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPE 140
           DL+KLRPMILKRI+NRAK+YP+K ++PVA+++L AR  L+  ++ LLK FPV++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 141 VYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNF 200
           V+VG++GHLI +C  Y R   N++HEW+ G +NDILVPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 201 ERVPAVVELCWQAGANPNDENLSSNTWNSDGNGLGRD-EPLSSHELRLLANTTIRAWETL 260
           +RVPA++ELC QAGA   +E L  +  + +      D   L + +L+ +    + AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 261 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPK 320
           R GV+KLL+VYP+KVCK C EVHVGPSGH ARLCGVFKYESWRG+H WEKA V+DLVP K
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 321 IVWHRRPQDPPVLVDEGRDYYGHTPAVIALCTQAGAIAPSKYYCMMKVQGLS 372
           +VWHRRPQDP VLVDEGR YYGH PA+++LC+  GAI P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of Spg000716 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 219.5 bits (558), Expect = 4.4e-57
Identity = 120/328 (36.59%), Postives = 172/328 (52.44%), Query Frame = 0

Query: 72  LQRCYSSKVDLKKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAF 131
           L+R    +V   K +P   KR     KN   +K ++P+A +V  AR  L++ +  L+K  
Sbjct: 102 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 161

Query: 132 PVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQD 191
            V +C +C E++VG  GH  +SC G     +  +HEW    + D++VP+EA+HL      
Sbjct: 162 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 221

Query: 192 VIKHDERFNFERVPAVVELCWQAGA-----------NPNDENLSSNTWNSDGNGLGRDE- 251
            I+HDERF+  RVPAVVELC Q G             P      S   ++D   L   E 
Sbjct: 222 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 281

Query: 252 ------------------PLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSE 311
                             P S  E   LA  T++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 282 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 341

Query: 312 VHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYY 369
           VHVGP+GH A+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R +Y
Sbjct: 342 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 401

BLAST of Spg000716 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 219.5 bits (558), Expect = 4.4e-57
Identity = 120/328 (36.59%), Postives = 172/328 (52.44%), Query Frame = 0

Query: 72  LQRCYSSKVDLKKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAF 131
           L+R    +V   K +P   KR     KN   +K ++P+A +V  AR  L++ +  L+K  
Sbjct: 105 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 164

Query: 132 PVVSCKFCPEVYVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQD 191
            V +C +C E++VG  GH  +SC G     +  +HEW    + D++VP+EA+HL      
Sbjct: 165 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 224

Query: 192 VIKHDERFNFERVPAVVELCWQAGA-----------NPNDENLSSNTWNSDGNGLGRDE- 251
            I+HDERF+  RVPAVVELC Q G             P      S   ++D   L   E 
Sbjct: 225 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 284

Query: 252 ------------------PLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSE 311
                             P S  E   LA  T++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 285 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 344

Query: 312 VHVGPSGHMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYY 369
           VHVGP+GH A+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R +Y
Sbjct: 345 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 404

BLAST of Spg000716 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 211.5 bits (537), Expect = 1.2e-54
Identity = 116/328 (35.37%), Postives = 170/328 (51.83%), Query Frame = 0

Query: 83  KKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPEV 142
           KKL  M +++  +  KN   +  ++PVA QV++   +L+ G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 143 YVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNFE 202
           +V   GH IR C G     +   H W+KG +ND+L+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 203 RVPAVVELCWQAGAN---------------------------------PNDENLSSNTWN 262
           R+PA+VELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 263 SDGNGL-GRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 322
            D  G+  R  P +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 323 HMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVI 376
           H  +LCG FK++   G H W+ A VD++ PP  VWH R      L    R +YG  PA++
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of Spg000716 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 211.5 bits (537), Expect = 1.2e-54
Identity = 116/328 (35.37%), Postives = 170/328 (51.83%), Query Frame = 0

Query: 83  KKLRPMILKRIQNRAKN-YPIKGMIPVAQQVLEARTMLMHGVSTLLKAFPVVSCKFCPEV 142
           KKL  M +++  +  KN   +  ++PVA QV++   +L+ G++ LL   PV +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 143 YVGEKGHLIRSCGGYKRGAKNKVHEWIKGDLNDILVPVEAFHLHHMFQDVIKHDERFNFE 202
           +V   GH IR C G     +   H W+KG +ND+L+PVE++H++  F   IKH+ RF +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 203 RVPAVVELCWQAGAN---------------------------------PNDENLSSNTWN 262
           R+PA+VELC QAG                                       +LSS    
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 263 SDGNGL-GRDEPLSSHELRLLANTTIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 322
            D  G+  R  P +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 323 HMARLCGVFKYESWRGSHRWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGHTPAVI 376
           H  +LCG FK++   G H W+ A VD++ PP  VWH R      L    R +YG  PA++
Sbjct: 367 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023532078.19.6e-17188.69APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022971049.11.6e-17088.92APO protein 4, mitochondrial-like [Cucurbita maxima][more]
XP_038901944.13.6e-17086.97APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ... [more]
XP_022947979.14.8e-17088.62APO protein 4, mitochondrial-like [Cucurbita moschata][more]
KAG6604849.15.3e-16988.31APO protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LSZ02.1e-10459.59APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2[more]
Q8W4A56.2e-5636.59APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1[more]
Q9XIR41.7e-5335.37APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1[more]
Q9FH509.4e-4433.68APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I7G77.9e-17188.92APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE... [more]
A0A6J1G8H92.3e-17088.62APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 ... [more]
A0A0A0LQG57.2e-16484.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1[more]
A0A5A7TJ186.1e-16384.45APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180... [more]
A0A6J1CIR91.0e-16285.89APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4... [more]
Match NameE-valueIdentityDescription
AT3G21740.11.5e-10559.59Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.14.4e-5736.59Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.24.4e-5736.59Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.11.2e-5435.37Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.21.2e-5435.37Arabidopsis thaliana protein of unknown function (DUF794) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023342APO domainPFAMPF05634APO_RNA-bindcoord: 78..216
e-value: 2.8E-41
score: 141.1
coord: 246..355
e-value: 2.2E-19
score: 69.7
IPR023342APO domainPROSITEPS51499APOcoord: 274..359
score: 22.174015
IPR023342APO domainPROSITEPS51499APOcoord: 134..219
score: 22.776535
NoneNo IPR availablePANTHERPTHR10388EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1coord: 62..373
NoneNo IPR availablePANTHERPTHR10388:SF20APO PROTEIN 4, MITOCHONDRIALcoord: 62..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000716.1Spg000716.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding