Spg000687 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000687
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGDSL esterase/lipase
Locationscaffold8: 45829667 .. 45831186 (-)
RNA-Seq ExpressionSpg000687
SyntenySpg000687
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACATCTTGTTTATCCATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGGCAGCCCCTAGTTCCTGCATTGTTCACATTTGGGGACTCTGTCCTCGATGTGGGGATCAATAATCACCTCAAAACCATCATTAAAGCAAACTTCCTCCCTTATGGAAGAGATTTCATTACTCACAAGCCAACAGGAAGGTTCTGTAATGGAAAACTTGCCTCCGATTTCACTGGTGCTTTGTACTTTAGAAACTCATTTCACTTGCTTATGTTCATATATAATCAATTCTTGATCTAATTAGATCTAATTTATTGTATTGTCTTCTGAATAGCTGAGTATCTGGGATTTACATCTTACCCACAACCTTACCTCGATGAAGCAGGAGGGAAGAACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCGTCTGGATACCTTGACTCAACAGCAGAGTTATATGTAATACTGTAAACCAAAAAATTGAATTTATCTCTTAGGCACTGTCCTAAAATAATGGGCTTCTGGTATTGTAGAATGCACTTTCCCTAACTCAGCAGCTGGAATACTACAAGGAATATCAGAACAAAGTAGCCAATTTTGCAGGGAAACCCAACGCTTCATCAATAGTATCTGGTGCAATTTACCTTGTAAGTGCAGGGAGCAACGACTTTCTGCAGAACTATTACATCAATCCTCTACTTTACAAGAAATATACTGTTCATCAGTTTGCTGACATTCTCATCACCTCCTACATAAATTTCATTCAGGTCTTGCTCTCCATCAAACTCCTAAATGTAAATACGAAAATATTTATATGAATATTGTATAATCAAAACATATGATTTCAGAGTCTATATGCATTGGGAGCACGGAGGATTGGAGTGACAACATTGCCACCGCTTGGATGTCTTCCGTCAGCCATAACAATGTTTGGATCTGACAGCAACGAGTGTGTGGCTAAGCTAAACAATGATGCAGTTTCATTCAATAGCAAGCTCAATGCCACATCTCAGCGCTTACATACCAAGCTTTCTGGCCTCAATCTGGTTGTCTTGGACACTTACAAACCTCTCTATGACCTTGTCACAAAGCCCGCTGAAAATGGTATGCCATTAAGATAATAAACATGCAACTACTCCAATCTAGGATATACGGTACAGTACAGGTTAATGGTCCATACAATATCTTAAATAAAGTAGAATGTATATGTCATGGAATCTTTCACATAGAAGCACAAAAGACTGAAAATCCCATTTATGCTTTAAAACTTGACCATCAGAATCCAAGACGTATCATTAACTCATGCAAAAAATTATGTAGGTTTCTTTGAGGCAAGGAAGGCCTGCTGCGGCACAGGCTTGCTTGAAACATCAATCCTATGCAACACAGAATCCGTAGGTACATGTGCCAATGCATCTGAATACGTGTTCTGGGATGGATTCCACCCATCAGAAGCAGCAAACAAGTTCTTGGCTGATAATCTGCTAGCCAGTGGCATCTCTCTCATCTCATAA

mRNA sequence

ATGGATACATCTTGTTTATCCATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGGCAGCCCCTAGTTCCTGCATTGTTCACATTTGGGGACTCTGTCCTCGATGTGGGGATCAATAATCACCTCAAAACCATCATTAAAGCAAACTTCCTCCCTTATGGAAGAGATTTCATTACTCACAAGCCAACAGGAAGGTTCTGTAATGGAAAACTTGCCTCCGATTTCACTGCTGAGTATCTGGGATTTACATCTTACCCACAACCTTACCTCGATGAAGCAGGAGGGAAGAACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCGTCTGGATACCTTGACTCAACAGCAGAGTTATATAATGCACTTTCCCTAACTCAGCAGCTGGAATACTACAAGGAATATCAGAACAAAGTAGCCAATTTTGCAGGGAAACCCAACGCTTCATCAATAGTATCTGGTGCAATTTACCTTAGTCTATATGCATTGGGAGCACGGAGGATTGGAGTGACAACATTGCCACCGCTTGGATGTCTTCCGTCAGCCATAACAATGTTTGGATCTGACAGCAACGAGTGTGTGGCTAAGCTAAACAATGATGCAGTTTCATTCAATAGCAAGCTCAATGCCACATCTCAGCGCTTACATACCAAGCTTTCTGGCCTCAATCTGGTTGTCTTGGACACTTACAAACCTCTCTATGACCTTGTCACAAAGCCCGCTGAAAATGGTTTCTTTGAGGCAAGGAAGGCCTGCTGCGGCACAGGCTTGCTTGAAACATCAATCCTATGCAACACAGAATCCGTAGGTACATGTGCCAATGCATCTGAATACGTGTTCTGGGATGGATTCCACCCATCAGAAGCAGCAAACAAGTTCTTGGCTGATAATCTGCTAGCCAGTGGCATCTCTCTCATCTCATAA

Coding sequence (CDS)

ATGGATACATCTTGTTTATCCATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGGCAGCCCCTAGTTCCTGCATTGTTCACATTTGGGGACTCTGTCCTCGATGTGGGGATCAATAATCACCTCAAAACCATCATTAAAGCAAACTTCCTCCCTTATGGAAGAGATTTCATTACTCACAAGCCAACAGGAAGGTTCTGTAATGGAAAACTTGCCTCCGATTTCACTGCTGAGTATCTGGGATTTACATCTTACCCACAACCTTACCTCGATGAAGCAGGAGGGAAGAACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCGTCTGGATACCTTGACTCAACAGCAGAGTTATATAATGCACTTTCCCTAACTCAGCAGCTGGAATACTACAAGGAATATCAGAACAAAGTAGCCAATTTTGCAGGGAAACCCAACGCTTCATCAATAGTATCTGGTGCAATTTACCTTAGTCTATATGCATTGGGAGCACGGAGGATTGGAGTGACAACATTGCCACCGCTTGGATGTCTTCCGTCAGCCATAACAATGTTTGGATCTGACAGCAACGAGTGTGTGGCTAAGCTAAACAATGATGCAGTTTCATTCAATAGCAAGCTCAATGCCACATCTCAGCGCTTACATACCAAGCTTTCTGGCCTCAATCTGGTTGTCTTGGACACTTACAAACCTCTCTATGACCTTGTCACAAAGCCCGCTGAAAATGGTTTCTTTGAGGCAAGGAAGGCCTGCTGCGGCACAGGCTTGCTTGAAACATCAATCCTATGCAACACAGAATCCGTAGGTACATGTGCCAATGCATCTGAATACGTGTTCTGGGATGGATTCCACCCATCAGAAGCAGCAAACAAGTTCTTGGCTGATAATCTGCTAGCCAGTGGCATCTCTCTCATCTCATAA

Protein sequence

MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYLSLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLNNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS
Homology
BLAST of Spg000687 vs. NCBI nr
Match: XP_038901478.1 (GDSL esterase/lipase At5g22810 [Benincasa hispida])

HSP 1 Score: 551.2 bits (1419), Expect = 5.6e-153
Identity = 286/349 (81.95%), Postives = 299/349 (85.67%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT CLSIFF LLAPVI+LANGQPLVPALFTFGDSVLDVGINNHLKT+IKA+FLPYGRDF
Sbjct: 1   MDTICLSIFF-LLAPVITLANGQPLVPALFTFGDSVLDVGINNHLKTVIKADFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
           ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYL  +GGKNLLIGASFASAASGYLD+TAE
Sbjct: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYL--SGGKNLLIGASFASAASGYLDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LYNALSLTQQLEYYKEYQNKVA  AGK NASSI+SGAIYL                    
Sbjct: 121 LYNALSLTQQLEYYKEYQNKVAEIAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LYA+GARRIGVTTLPPLGCLP+AIT+FGSDSNECVAKLN
Sbjct: 181 KYTANQFGNIIITSYINFIQNLYAMGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAVSFNSKLNATSQ L TKLSGLNLVVLD+YKPLYDL+TKPAENGFFE+RKACCGTGLL
Sbjct: 241 NDAVSFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAENGFFESRKACCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLA NLLASGISLIS
Sbjct: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLASNLLASGISLIS 346

BLAST of Spg000687 vs. NCBI nr
Match: XP_023534530.1 (GDSL esterase/lipase At5g22810-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 278/350 (79.43%), Postives = 294/350 (84.00%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRD 60
           MD +CL+IFFLLLAP+  SLANGQPLVPA+FTFGDSVLDVGINNHLKT++KANF PYGRD
Sbjct: 1   MDVTCLAIFFLLLAPLSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTA 120
           F+TH PTGRFCNGKLASDFTA+YLGFTSYPQ YL EAGGKNLLIGASFASAASGYLD+TA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTADYLGFTSYPQAYLGEAGGKNLLIGASFASAASGYLDTTA 120

Query: 121 ELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL------------------- 180
            LYNALSLTQQLEYYKEYQNKVA FAGKPNAS+I+SGAIYL                   
Sbjct: 121 HLYNALSLTQQLEYYKEYQNKVAEFAGKPNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 ---------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKL 240
                                +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFADILIASYINFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGL 300
           NNDAVSFNSKLNATSQ LH +L GLNLVVLDTYKPLY LVTKPAENGFFEARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHAELPGLNLVVLDTYKPLYQLVTKPAENGFFEARKACCGTGL 300

Query: 301 LETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           LETSILCN ESVGTCANASEYVFWDGFHPSEAANKFLADNLL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of Spg000687 vs. NCBI nr
Match: XP_022958433.1 (GDSL esterase/lipase At5g22810-like [Cucurbita moschata])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 279/350 (79.71%), Postives = 291/350 (83.14%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRD 60
           MD +CL IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKT++KANF PYGRD
Sbjct: 1   MDVTCLPIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQ YL EAGGKNLLIGASFASAASGYLD+TA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGASFASAASGYLDTTA 120

Query: 121 ELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL------------------- 180
            LYNALSLTQQLEYYKEYQNKVA FAGK NAS+I+SGAIYL                   
Sbjct: 121 HLYNALSLTQQLEYYKEYQNKVAEFAGKTNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 ---------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKL 240
                                +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFADILIASYVNFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGL 300
           NNDAVSFNSKLNATSQ LH  L GLNLVVLDTYKPLY LVTKPAENGFFEARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYQLVTKPAENGFFEARKACCGTGL 300

Query: 301 LETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           LETSILCN ESVGTCANASEYVFWDGFHPSEAANKFLADNLL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of Spg000687 vs. NCBI nr
Match: XP_008458651.1 (PREDICTED: GDSL esterase/lipase At5g22810 [Cucumis melo] >KAA0033359.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYJ96643.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 541.2 bits (1393), Expect = 5.8e-150
Identity = 279/349 (79.94%), Postives = 293/349 (83.95%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKT+IKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQ YL   GGK+ L+GASFASAASGYLD+TAE
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDFLVGASFASAASGYLDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LYNALS TQQLEYYKEYQNKVA  AGKPNASSI+SGAIYL                    
Sbjct: 121 LYNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KLN
Sbjct: 181 KYTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAV+FNSKLNATSQ L TKLSGLNLVVLD+YKPLYDL+TKPAE+GF EARKACCGTGLL
Sbjct: 241 NDAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETS LCNTESVGTCANASEYVFWDGFHPSEAANKFLA +LLASGISLIS
Sbjct: 301 ETSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of Spg000687 vs. NCBI nr
Match: XP_022140793.1 (GDSL esterase/lipase At5g22810 [Momordica charantia])

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-149
Identity = 274/349 (78.51%), Postives = 294/349 (84.24%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSI FL+L PVISLANGQPLVPALFTFGDSVLDVG NNHLKTI+KANFLPYGRDF
Sbjct: 1   MDTTCLSILFLVLTPVISLANGQPLVPALFTFGDSVLDVGNNNHLKTIVKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
           ITHKPTGRFCNGK++SDFTAEYLGFT+YP+ YL EAGG+NLLIG+SFASAASGY D+TAE
Sbjct: 61  ITHKPTGRFCNGKISSDFTAEYLGFTTYPKAYLSEAGGENLLIGSSFASAASGYHDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LY+ALSLTQQLEY+KEYQNKV  FAGK NASSI+SGAIYL                    
Sbjct: 121 LYDALSLTQQLEYFKEYQNKVTEFAGKTNASSIISGAIYLVSVGSNDFVQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LY+LGAR+IGVTTLPPLGCLP+AIT+FGSDSNECVAKLN
Sbjct: 181 KYTVHQFANILITSYINFIENLYSLGARKIGVTTLPPLGCLPAAITIFGSDSNECVAKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAVSFNSKLNATS+ L TKL GLNLVVLDTY+PLYDLVTKPAENGFFEARK CCGTGLL
Sbjct: 241 NDAVSFNSKLNATSRSLQTKLPGLNLVVLDTYQPLYDLVTKPAENGFFEARKGCCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETSILCNTESVGTC NASEYVFWDGFHPSEAANKFLADNLL SGISLIS
Sbjct: 301 ETSILCNTESVGTCTNASEYVFWDGFHPSEAANKFLADNLLTSGISLIS 349

BLAST of Spg000687 vs. ExPASy Swiss-Prot
Match: Q9FFC6 (GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3 SV=3)

HSP 1 Score: 402.1 bits (1032), Expect = 5.6e-111
Identity = 210/338 (62.13%), Postives = 243/338 (71.89%), Query Frame = 0

Query: 16  VISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKPTGRFCNGKLA 75
           V+ +   QPLVPA+F FGDSV+DVG NN + TI+KANF PYGRDF TH PTGRFCNGKLA
Sbjct: 25  VMVVMKAQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLA 84

Query: 76  SDFTAEYLGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDSTAELYNALSLTQQLEYY 135
           +DFTAE LGF SYPQ YL  +A GKNLLIGA+FASAASGY D TA+LY+A+SL QQLE+Y
Sbjct: 85  TDFTAENLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHY 144

Query: 136 KEYQNKVANFA---GKPNASSIVSGAIYL------------------------------- 195
           K+Y +++   A      NAS+I+S  IY+                               
Sbjct: 145 KDYISRIQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLL 204

Query: 196 ---------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLNNDAVSFNSKLN 255
                    +LY+LGARRIGVTTLPPLGCLP+AIT+ G     C  KLNNDA+SFN+KLN
Sbjct: 205 ILSYSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLN 264

Query: 256 ATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLETSILCNTESV 310
            TSQ L   L GLNLVV D Y+PLYDL T+P+E GF EAR+ACCGTGLLETSILCN +SV
Sbjct: 265 TTSQDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSV 324

BLAST of Spg000687 vs. ExPASy Swiss-Prot
Match: Q9LZC5 (GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.3e-103
Identity = 198/347 (57.06%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 6   LSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKP 65
           L  F ++      +  G+PLVPAL   GDSV+D G NN L T+IKANF PYGRDF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 66  TGRFCNGKLASDFTAEYLGFTSYPQPYL-DEAGGKNLLIGASFASAASGYLDSTAELYNA 125
           TGRF NGKLA+DFTAE LGFTSYP PYL  EA G NLL GA+FAS ASGY D TA  YNA
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 126 LSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL------------------------ 185
           ++L QQL+ YKEYQNKV N  G   A+ I SGAI+L                        
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 186 ----------------SLYALGARRIGVTTLPPLGCLPSAITMFG--SDSNECVAKLNND 245
                           +LY LGAR+IGVTTLPPLGCLP+AIT+FG   ++N CV +LN D
Sbjct: 188 DQYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQD 247

Query: 246 AVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLET 305
           AVSFN+KLN TS  L   L GL LVV D Y PL ++   P ENGFFE+R+ACCGTG +ET
Sbjct: 248 AVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVET 307

Query: 306 SILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           S LCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A+NLL  GI LIS
Sbjct: 308 SFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 354

BLAST of Spg000687 vs. ExPASy Swiss-Prot
Match: Q9FFN0 (GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-100
Identity = 198/347 (57.06%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 5   CLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHK 64
           CLS+     A V     G+ LVPAL   GDSV+D G NNH  T++KANF PYGRDF+ H 
Sbjct: 10  CLSVIACFYAGV---GTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHS 69

Query: 65  PTGRFCNGKLASDFTAEYLGFTSYPQPYL-DEAGGKNLLIGASFASAASGYLDSTAELYN 124
            TGRF NGKLA+DFTAE LGFTSYP  YL  EA   NLL GA+FAS ASG+ D+TA  YN
Sbjct: 70  ATGRFSNGKLATDFTAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYN 129

Query: 125 ALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------------- 184
           A++L+QQL+ YKEYQNKV N  GK  A+ I SGAI+L                       
Sbjct: 130 AITLSQQLKNYKEYQNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFT 189

Query: 185 -----------------SLYALGARRIGVTTLPPLGCLPSAITMFGS-DSNECVAKLNND 244
                            +LY LGARRIGVTTLPPLGCLP+AIT+FG   +N CV +LN D
Sbjct: 190 PDQYSDHLLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQD 249

Query: 245 AVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLET 304
           AVSFN+KLN TS  L   L GL LVV D Y PL ++V  P E GFFE+R+ACCGTG +ET
Sbjct: 250 AVSFNTKLNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMET 309

Query: 305 SILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           S LCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A+NLL  GI LIS
Sbjct: 310 SFLCNALSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 353

BLAST of Spg000687 vs. ExPASy Swiss-Prot
Match: Q0WPI9 (GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.8e-94
Identity = 173/340 (50.88%), Postives = 227/340 (66.76%), Query Frame = 0

Query: 11  LLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKPTGRFC 70
           LLL       +   +VPAL  FGDS++DVG NN+L +I+K+NFLPYGRDFI  +PTGRFC
Sbjct: 12  LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFC 71

Query: 71  NGKLASDFTAEYLGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDSTAELYNALSLTQ 130
           NGKLA DF+AEYLGF+SYP  +L  EA  +N+LIGA+FASA+SGY D+T+  + ++SLT+
Sbjct: 72  NGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTR 131

Query: 131 QLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------------------- 190
           QL YY+ YQN+V    G+ NA  + S  I++                             
Sbjct: 132 QLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFAD 191

Query: 191 -----------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLNNDAVSFNSK 250
                      +LY LGARRIGV +LPP+GCLP+AIT+FG+ +  CV +LNNDA+ FN+K
Sbjct: 192 ILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTK 251

Query: 251 LNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLETSILCNTE 310
           L  T++ L  + SGL LV  + Y+P  D++T P +NGFFE ++ACCGTG +ETS LCN+ 
Sbjct: 252 LENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSL 311

BLAST of Spg000687 vs. ExPASy Swiss-Prot
Match: Q9LU14 (GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.9e-91
Identity = 180/352 (51.14%), Postives = 225/352 (63.92%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISL--ANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGR 60
           MD    S   L L   +S+   +   LVPA+ TFGDSV+DVG NN+L T+ +A++ PYGR
Sbjct: 1   MDRCTSSFLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGR 60

Query: 61  DFITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDS 120
           DF  HK TGRFCNGKLA+D TAE LGFT YP  YL  EA GKNLLIGA+FASAASGY D 
Sbjct: 61  DFANHKATGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDK 120

Query: 121 TAELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------- 180
            A L +A+ L QQ+EY+KEY++K+   AG   A SI+ GAI L                 
Sbjct: 121 AALLNHAIPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPL 180

Query: 181 -----------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVA 240
                                   +YA+GAR+IGVT+LPP GCLP+A T+FG     CV+
Sbjct: 181 LYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVS 240

Query: 241 KLNNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGT 300
           +LN DA +FN KLNA + +L  + S L +VV D Y PLYDLV  P+++GF EA K CCGT
Sbjct: 241 RLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGT 300

Query: 301 GLLE-TSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLI 309
           G +E TS+LCN +S GTC+NA++YVFWD  HPSEAAN+ LA  L+  G SL+
Sbjct: 301 GTVETTSLLCNPKSFGTCSNATQYVFWDSVHPSEAANEILATALIGQGFSLL 352

BLAST of Spg000687 vs. ExPASy TrEMBL
Match: A0A6J1H329 (GDSL esterase/lipase At5g22810-like OS=Cucurbita moschata OX=3662 GN=LOC111459655 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.2e-150
Identity = 279/350 (79.71%), Postives = 291/350 (83.14%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRD 60
           MD +CL IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKT++KANF PYGRD
Sbjct: 1   MDVTCLPIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQ YL EAGGKNLLIGASFASAASGYLD+TA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGASFASAASGYLDTTA 120

Query: 121 ELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL------------------- 180
            LYNALSLTQQLEYYKEYQNKVA FAGK NAS+I+SGAIYL                   
Sbjct: 121 HLYNALSLTQQLEYYKEYQNKVAEFAGKTNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 ---------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKL 240
                                +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFADILIASYVNFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGL 300
           NNDAVSFNSKLNATSQ LH  L GLNLVVLDTYKPLY LVTKPAENGFFEARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYQLVTKPAENGFFEARKACCGTGL 300

Query: 301 LETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           LETSILCN ESVGTCANASEYVFWDGFHPSEAANKFLADNLL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of Spg000687 vs. ExPASy TrEMBL
Match: A0A5A7SW04 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00080 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.8e-150
Identity = 279/349 (79.94%), Postives = 293/349 (83.95%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKT+IKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQ YL   GGK+ L+GASFASAASGYLD+TAE
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDFLVGASFASAASGYLDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LYNALS TQQLEYYKEYQNKVA  AGKPNASSI+SGAIYL                    
Sbjct: 121 LYNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KLN
Sbjct: 181 KYTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAV+FNSKLNATSQ L TKLSGLNLVVLD+YKPLYDL+TKPAE+GF EARKACCGTGLL
Sbjct: 241 NDAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETS LCNTESVGTCANASEYVFWDGFHPSEAANKFLA +LLASGISLIS
Sbjct: 301 ETSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of Spg000687 vs. ExPASy TrEMBL
Match: A0A1S3C7W6 (GDSL esterase/lipase At5g22810 OS=Cucumis melo OX=3656 GN=LOC103497987 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.8e-150
Identity = 279/349 (79.94%), Postives = 293/349 (83.95%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKT+IKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQ YL   GGK+ L+GASFASAASGYLD+TAE
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDFLVGASFASAASGYLDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LYNALS TQQLEYYKEYQNKVA  AGKPNASSI+SGAIYL                    
Sbjct: 121 LYNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECV KLN
Sbjct: 181 KYTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAV+FNSKLNATSQ L TKLSGLNLVVLD+YKPLYDL+TKPAE+GF EARKACCGTGLL
Sbjct: 241 NDAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETS LCNTESVGTCANASEYVFWDGFHPSEAANKFLA +LLASGISLIS
Sbjct: 301 ETSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of Spg000687 vs. ExPASy TrEMBL
Match: A0A0A0KAZ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149950 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.8e-150
Identity = 280/349 (80.23%), Postives = 295/349 (84.53%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKT+IKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
           ITHKPTGRFCNGKLASDFTAEYLGFTSYPQ YL   GGK+LLIGASFASAASGYLD+TAE
Sbjct: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDLLIGASFASAASGYLDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LYNALS TQQLE+YKEYQNKVA  AGK NASSI+SGAIYL                    
Sbjct: 121 LYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LYALGARRIGVTTLPPLGCLP+AIT+FGSDSNECVAKLN
Sbjct: 181 KYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAV+FNSKLNATSQ L TKL GLNLVVLD+YKPLYDL+TKPAE+GF EARKACCGTGLL
Sbjct: 241 NDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETS LCNTESVGTCANAS+YVFWDGFHPSEAANKFLA +LLASGISLIS
Sbjct: 301 ETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of Spg000687 vs. ExPASy TrEMBL
Match: A0A6J1CIU1 (GDSL esterase/lipase At5g22810 OS=Momordica charantia OX=3673 GN=LOC111011369 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.8e-150
Identity = 274/349 (78.51%), Postives = 294/349 (84.24%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDF 60
           MDT+CLSI FL+L PVISLANGQPLVPALFTFGDSVLDVG NNHLKTI+KANFLPYGRDF
Sbjct: 1   MDTTCLSILFLVLTPVISLANGQPLVPALFTFGDSVLDVGNNNHLKTIVKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLDEAGGKNLLIGASFASAASGYLDSTAE 120
           ITHKPTGRFCNGK++SDFTAEYLGFT+YP+ YL EAGG+NLLIG+SFASAASGY D+TAE
Sbjct: 61  ITHKPTGRFCNGKISSDFTAEYLGFTTYPKAYLSEAGGENLLIGSSFASAASGYHDTTAE 120

Query: 121 LYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL-------------------- 180
           LY+ALSLTQQLEY+KEYQNKV  FAGK NASSI+SGAIYL                    
Sbjct: 121 LYDALSLTQQLEYFKEYQNKVTEFAGKTNASSIISGAIYLVSVGSNDFVQNYYINPLLYK 180

Query: 181 --------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLN 240
                               +LY+LGAR+IGVTTLPPLGCLP+AIT+FGSDSNECVAKLN
Sbjct: 181 KYTVHQFANILITSYINFIENLYSLGARKIGVTTLPPLGCLPAAITIFGSDSNECVAKLN 240

Query: 241 NDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLL 300
           NDAVSFNSKLNATS+ L TKL GLNLVVLDTY+PLYDLVTKPAENGFFEARK CCGTGLL
Sbjct: 241 NDAVSFNSKLNATSRSLQTKLPGLNLVVLDTYQPLYDLVTKPAENGFFEARKGCCGTGLL 300

Query: 301 ETSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           ETSILCNTESVGTC NASEYVFWDGFHPSEAANKFLADNLL SGISLIS
Sbjct: 301 ETSILCNTESVGTCTNASEYVFWDGFHPSEAANKFLADNLLTSGISLIS 349

BLAST of Spg000687 vs. TAIR 10
Match: AT5G22810.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 8.8e-112
Identity = 209/331 (63.14%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 23  QPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKPTGRFCNGKLASDFTAEY 82
           QPLVPA+F FGDSV+DVG NN + TI+KANF PYGRDF TH PTGRFCNGKLA+DFTAE 
Sbjct: 7   QPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLATDFTAEN 66

Query: 83  LGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDSTAELYNALSLTQQLEYYKEYQNKV 142
           LGF SYPQ YL  +A GKNLLIGA+FASAASGY D TA+LY+A+SL QQLE+YK+Y +++
Sbjct: 67  LGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHYKDYISRI 126

Query: 143 ANFA---GKPNASSIVSGAIYL-------------------------------------- 202
              A      NAS+I+S  IY+                                      
Sbjct: 127 QEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLLILSYSSF 186

Query: 203 --SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLNNDAVSFNSKLNATSQRLH 262
             +LY+LGARRIGVTTLPPLGCLP+AIT+ G     C  KLNNDA+SFN+KLN TSQ L 
Sbjct: 187 IQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLNTTSQDLK 246

Query: 263 TKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLETSILCNTESVGTCANAS 310
             L GLNLVV D Y+PLYDL T+P+E GF EAR+ACCGTGLLETSILCN +SVGTC NA+
Sbjct: 247 RNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVGTCNNAT 306

BLAST of Spg000687 vs. TAIR 10
Match: AT5G03820.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 376.3 bits (965), Expect = 2.3e-104
Identity = 198/347 (57.06%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 6   LSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKP 65
           L  F ++      +  G+PLVPAL   GDSV+D G NN L T+IKANF PYGRDF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 66  TGRFCNGKLASDFTAEYLGFTSYPQPYL-DEAGGKNLLIGASFASAASGYLDSTAELYNA 125
           TGRF NGKLA+DFTAE LGFTSYP PYL  EA G NLL GA+FAS ASGY D TA  YNA
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 126 LSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL------------------------ 185
           ++L QQL+ YKEYQNKV N  G   A+ I SGAI+L                        
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 186 ----------------SLYALGARRIGVTTLPPLGCLPSAITMFG--SDSNECVAKLNND 245
                           +LY LGAR+IGVTTLPPLGCLP+AIT+FG   ++N CV +LN D
Sbjct: 188 DQYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQD 247

Query: 246 AVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLET 305
           AVSFN+KLN TS  L   L GL LVV D Y PL ++   P ENGFFE+R+ACCGTG +ET
Sbjct: 248 AVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVET 307

Query: 306 SILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           S LCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A+NLL  GI LIS
Sbjct: 308 SFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 354

BLAST of Spg000687 vs. TAIR 10
Match: AT5G03810.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 367.9 bits (943), Expect = 8.3e-102
Identity = 198/347 (57.06%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 5   CLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHK 64
           CLS+     A V     G+ LVPAL   GDSV+D G NNH  T++KANF PYGRDF+ H 
Sbjct: 10  CLSVIACFYAGV---GTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHS 69

Query: 65  PTGRFCNGKLASDFTAEYLGFTSYPQPYL-DEAGGKNLLIGASFASAASGYLDSTAELYN 124
            TGRF NGKLA+DFTAE LGFTSYP  YL  EA   NLL GA+FAS ASG+ D+TA  YN
Sbjct: 70  ATGRFSNGKLATDFTAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYN 129

Query: 125 ALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------------- 184
           A++L+QQL+ YKEYQNKV N  GK  A+ I SGAI+L                       
Sbjct: 130 AITLSQQLKNYKEYQNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFT 189

Query: 185 -----------------SLYALGARRIGVTTLPPLGCLPSAITMFGS-DSNECVAKLNND 244
                            +LY LGARRIGVTTLPPLGCLP+AIT+FG   +N CV +LN D
Sbjct: 190 PDQYSDHLLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQD 249

Query: 245 AVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLET 304
           AVSFN+KLN TS  L   L GL LVV D Y PL ++V  P E GFFE+R+ACCGTG +ET
Sbjct: 250 AVSFNTKLNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMET 309

Query: 305 SILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLIS 310
           S LCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A+NLL  GI LIS
Sbjct: 310 SFLCNALSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 353

BLAST of Spg000687 vs. TAIR 10
Match: AT3G53100.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-95
Identity = 173/340 (50.88%), Postives = 227/340 (66.76%), Query Frame = 0

Query: 11  LLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGRDFITHKPTGRFC 70
           LLL       +   +VPAL  FGDS++DVG NN+L +I+K+NFLPYGRDFI  +PTGRFC
Sbjct: 12  LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFC 71

Query: 71  NGKLASDFTAEYLGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDSTAELYNALSLTQ 130
           NGKLA DF+AEYLGF+SYP  +L  EA  +N+LIGA+FASA+SGY D+T+  + ++SLT+
Sbjct: 72  NGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTR 131

Query: 131 QLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------------------- 190
           QL YY+ YQN+V    G+ NA  + S  I++                             
Sbjct: 132 QLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFAD 191

Query: 191 -----------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVAKLNNDAVSFNSK 250
                      +LY LGARRIGV +LPP+GCLP+AIT+FG+ +  CV +LNNDA+ FN+K
Sbjct: 192 ILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTK 251

Query: 251 LNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGTGLLETSILCNTE 310
           L  T++ L  + SGL LV  + Y+P  D++T P +NGFFE ++ACCGTG +ETS LCN+ 
Sbjct: 252 LENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSL 311

BLAST of Spg000687 vs. TAIR 10
Match: AT3G16370.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.0e-92
Identity = 180/352 (51.14%), Postives = 225/352 (63.92%), Query Frame = 0

Query: 1   MDTSCLSIFFLLLAPVISL--ANGQPLVPALFTFGDSVLDVGINNHLKTIIKANFLPYGR 60
           MD    S   L L   +S+   +   LVPA+ TFGDSV+DVG NN+L T+ +A++ PYGR
Sbjct: 1   MDRCTSSFLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGR 60

Query: 61  DFITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLD-EAGGKNLLIGASFASAASGYLDS 120
           DF  HK TGRFCNGKLA+D TAE LGFT YP  YL  EA GKNLLIGA+FASAASGY D 
Sbjct: 61  DFANHKATGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDK 120

Query: 121 TAELYNALSLTQQLEYYKEYQNKVANFAGKPNASSIVSGAIYL----------------- 180
            A L +A+ L QQ+EY+KEY++K+   AG   A SI+ GAI L                 
Sbjct: 121 AALLNHAIPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPL 180

Query: 181 -----------------------SLYALGARRIGVTTLPPLGCLPSAITMFGSDSNECVA 240
                                   +YA+GAR+IGVT+LPP GCLP+A T+FG     CV+
Sbjct: 181 LYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVS 240

Query: 241 KLNNDAVSFNSKLNATSQRLHTKLSGLNLVVLDTYKPLYDLVTKPAENGFFEARKACCGT 300
           +LN DA +FN KLNA + +L  + S L +VV D Y PLYDLV  P+++GF EA K CCGT
Sbjct: 241 RLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGT 300

Query: 301 GLLE-TSILCNTESVGTCANASEYVFWDGFHPSEAANKFLADNLLASGISLI 309
           G +E TS+LCN +S GTC+NA++YVFWD  HPSEAAN+ LA  L+  G SL+
Sbjct: 301 GTVETTSLLCNPKSFGTCSNATQYVFWDSVHPSEAANEILATALIGQGFSLL 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901478.15.6e-15381.95GDSL esterase/lipase At5g22810 [Benincasa hispida][more]
XP_023534530.11.2e-15079.43GDSL esterase/lipase At5g22810-like [Cucurbita pepo subsp. pepo][more]
XP_022958433.14.5e-15079.71GDSL esterase/lipase At5g22810-like [Cucurbita moschata][more]
XP_008458651.15.8e-15079.94PREDICTED: GDSL esterase/lipase At5g22810 [Cucumis melo] >KAA0033359.1 GDSL este... [more]
XP_022140793.11.0e-14978.51GDSL esterase/lipase At5g22810 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9FFC65.6e-11162.13GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3... [more]
Q9LZC53.3e-10357.06GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3... [more]
Q9FFN01.2e-10057.06GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3... [more]
Q0WPI92.8e-9450.88GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2... [more]
Q9LU142.9e-9151.14GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H3292.2e-15079.71GDSL esterase/lipase At5g22810-like OS=Cucurbita moschata OX=3662 GN=LOC11145965... [more]
A0A5A7SW042.8e-15079.94GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G... [more]
A0A1S3C7W62.8e-15079.94GDSL esterase/lipase At5g22810 OS=Cucumis melo OX=3656 GN=LOC103497987 PE=3 SV=1[more]
A0A0A0KAZ34.8e-15080.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149950 PE=3 SV=1[more]
A0A6J1CIU14.8e-15078.51GDSL esterase/lipase At5g22810 OS=Momordica charantia OX=3673 GN=LOC111011369 PE... [more]
Match NameE-valueIdentityDescription
AT5G22810.18.8e-11263.14GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03820.12.3e-10457.06GDSL-like Lipase/Acylhydrolase family protein [more]
AT5G03810.18.3e-10257.06GDSL-like Lipase/Acylhydrolase family protein [more]
AT3G53100.12.0e-9550.88GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G16370.12.0e-9251.14GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 19..157
e-value: 4.4E-19
score: 71.0
coord: 158..304
e-value: 8.2E-38
score: 132.6
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 29..299
e-value: 6.9E-35
score: 121.5
NoneNo IPR availablePANTHERPTHR45642:SF103GDSL-MOTIF LIPASEcoord: 8..160
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 8..160
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 161..308
NoneNo IPR availablePANTHERPTHR45642:SF103GDSL-MOTIF LIPASEcoord: 161..308
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 165..301
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 27..301
e-value: 5.05619E-117
score: 338.051

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000687.1Spg000687.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds