Spg000520 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000520
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Abhydrolase_3 domain-containing protein
Location	scaffold8: 36058745 .. 36059242 (-)
RNA-Seq Expression	Spg000520
Synteny	Spg000520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAGGGATGGTCCAGAGCCATGGCTGAACGAGCATGCAGATTTCAACCGAGTCTGTTTGGCCGGCGACAGCGCCGGCGCCAACATTTGCAATTACTTGGCTTCTCGAGCAGGATCATCGGCGGAGCAACTAGGCGGAGTGAAGTTGGTGGCTATGGCTTTGATTCACCCCTTCTTCGGCGACGGCGGAGAGAACAGGCAGTGGAAGTATTTGTGTCCAGCGACCAGCGGAGACGACGATCCTAGGCTGAAACCGGCCGCAGAGGATCTGGCAAAGTTGCCTTGTGGGAGAGTGAAGATTTTTCTGGCAGAGGAGGATTTTCTGAAGGTTGGAGCGATGAAGTACGACGAGGATTTGAAGAGGTGCGGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGACCATGTGTTTCATTTGAAGAAGCCAGACTGTGAGAATGCGTTTGATTTGACGGAAAAATTAGCCTCTTTCATCAGCTTGCATTAA

mRNA sequence

ATGAACAGGGATGGTCCAGAGCCATGGCTGAACGAGCATGCAGATTTCAACCGAGTCTGTTTGGCCGGCGACAGCGCCGGCGCCAACATTTGCAATTACTTGGCTTCTCGAGCAGGATCATCGGCGGAGCAACTAGGCGGAGTGAAGTTGGTGGCTATGGCTTTGATTCACCCCTTCTTCGGCGACGGCGGAGAGAACAGGCAGTGGAAGTATTTGTGTCCAGCGACCAGCGGAGACGACGATCCTAGGCTGAAACCGGCCGCAGAGGATCTGGCAAAGTTGCCTTGTGGGAGAGTGAAGATTTTTCTGGCAGAGGAGGATTTTCTGAAGGTTGGAGCGATGAAGTACGACGAGGATTTGAAGAGGTGCGGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGACCATGTGTTTCATTTGAAGAAGCCAGACTGTGAGAATGCGTTTGATTTGACGGAAAAATTAGCCTCTTTCATCAGCTTGCATTAA

Coding sequence (CDS)

ATGAACAGGGATGGTCCAGAGCCATGGCTGAACGAGCATGCAGATTTCAACCGAGTCTGTTTGGCCGGCGACAGCGCCGGCGCCAACATTTGCAATTACTTGGCTTCTCGAGCAGGATCATCGGCGGAGCAACTAGGCGGAGTGAAGTTGGTGGCTATGGCTTTGATTCACCCCTTCTTCGGCGACGGCGGAGAGAACAGGCAGTGGAAGTATTTGTGTCCAGCGACCAGCGGAGACGACGATCCTAGGCTGAAACCGGCCGCAGAGGATCTGGCAAAGTTGCCTTGTGGGAGAGTGAAGATTTTTCTGGCAGAGGAGGATTTTCTGAAGGTTGGAGCGATGAAGTACGACGAGGATTTGAAGAGGTGCGGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGACCATGTGTTTCATTTGAAGAAGCCAGACTGTGAGAATGCGTTTGATTTGACGGAAAAATTAGCCTCTTTCATCAGCTTGCATTAA

Protein sequence

MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFFGDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISLH

Homology

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BLAST of Spg000520 vs. NCBI nr
Match: XP_004145358.2 (2-hydroxyisoflavanone dehydratase [Cucumis sativus] >KGN61726.1 hypothetical protein Csa_006817 [Cucumis sativus])

HSP 1 Score: 256.9 bits (655), Expect = 1.2e-64
Identity = 121/164 (73.78%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDG EPWLN + DFNR+CLAGDSAGANIC+YLA+RA SSAE+LGG K+VAMALIHPFF
Sbjct: 144 VNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAEELGGAKVVAMALIHPFF 203

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDGGENR WKYLC  T       L+P  EDLAKL C RVKIFLAE DFLK G   Y+EDL
Sbjct: 204 GDGGENRLWKYLCSETK-----LLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDL 263

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GWNG VETVEHG E+HVFHLKKP+CE A DL EKLASFI+L
Sbjct: 264 KSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFINL 302

[Close]

BLAST of Spg000520 vs. NCBI nr
Match: XP_022986577.1 (probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 121/164 (73.78%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDGPEPWLNEHAD +R+CLAGDSAGANIC++LA RAGSSAEQLG VK+VA+ALIHPFF
Sbjct: 141 VNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGSSAEQLGRVKVVALALIHPFF 200

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG E+R WKYLC      D   L+P A+DLA LPC RVKIFLAE+D LK G   YDEDL
Sbjct: 201 GDGAEDRLWKYLC-----SDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDL 260

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GW GAVETVEHGGEDHVFHLK P+CE A DL EKLA FI+L
Sbjct: 261 KSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINL 299

[Close]

BLAST of Spg000520 vs. NCBI nr
Match: XP_023512082.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.2 bits (648), Expect = 7.6e-64
Identity = 120/164 (73.17%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDGPEPWLN+HAD +R+CLAGDSAGANIC++LA RAGSSAEQLG VK+VA+ALIHPFF
Sbjct: 141 VNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSAEQLGRVKVVALALIHPFF 200

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG  +R WKYLC      D   L+P A+DLA LPC RVKIFLAE+D LK G   YDEDL
Sbjct: 201 GDGAGDRLWKYLC-----SDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDL 260

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GWNGAVETVEHGGEDHVFHLK P+CE A DL EKLA FI+L
Sbjct: 261 KSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINL 299

[Close]

BLAST of Spg000520 vs. NCBI nr
Match: XP_008459211.1 (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo] >KAA0032058.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa] >TYK13561.1 2-hydroxyisoflavanone dehydratase-like [Cucumis melo var. makuwa])

HSP 1 Score: 250.0 bits (637), Expect = 1.4e-62
Identity = 117/164 (71.34%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDG EPWLN + DFNR+CLAGDSAGANIC+YLA+RA SS+E+LGG K+VAMALIHPFF
Sbjct: 144 VNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSSEELGGAKVVAMALIHPFF 203

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG E+R WKYLC  T       ++P  E+LAK+ C RVKIFLAE DFLK G   YDEDL
Sbjct: 204 GDGREDRLWKYLCSETR-----LVRPTKEELAKMGCKRVKIFLAENDFLKFGGRNYDEDL 263

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           KR GWNG VETVEHGGE+HVFHLK P+ ENA DL EKLASFI+L
Sbjct: 264 KRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFINL 302

[Close]

BLAST of Spg000520 vs. NCBI nr
Match: XP_022944136.1 (probable carboxylesterase 12 [Cucurbita moschata])

HSP 1 Score: 249.6 bits (636), Expect = 1.9e-62
Identity = 117/164 (71.34%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDGPEPWLN+HAD +R+CLAGDSAGANIC++LA RAG+SAEQLG VK+VA+ALIHPFF
Sbjct: 141 VNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSAEQLGRVKVVALALIHPFF 200

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG  +R WKYLC      D   L+P A+DLA LPC RVKIFLAE+D LK G   YDEDL
Sbjct: 201 GDGAGDRLWKYLC-----SDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDL 260

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GW GAVETVEHGGEDHVFHLK P+CE A DL EKL+ FI+L
Sbjct: 261 KSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINL 299

[Close]

BLAST of Spg000520 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 8.3e-29
Identity = 69/174 (39.66%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 7   EPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFFGD---- 66
           EPW+N++AD + + L GDSAGANI ++LA RA  S + L   K+  + +IHP+F      
Sbjct: 140 EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTL---KIKGIGMIHPYFWGTQPI 199

Query: 67  GGENRQ----------WKYLCPATSGDDDPRLKPAAE---DLAKLPCGRVKIFLAEEDFL 126
           G E +           W+++CP+  G DDP + P A+   DL  L C RV I +AE+D L
Sbjct: 200 GAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGLGCERVMITVAEKDIL 259

Query: 127 KVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFIS 164
                 Y E L +  W G VE +E   +DHVFH+ +PDC+ A ++   LA FI+
Sbjct: 260 NERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEMVRCLALFIN 310

[Close]

BLAST of Spg000520 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.1e-28
Identity = 71/179 (39.66%), Postives = 99/179 (55.31%), Query Frame = 0


Query: 5   GPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSS--AEQLGGVKLVAMALIHPFFG- 64
           G E WLN+HADF+RV L+GDSAGANI +++A RA     +  L    +  + L+HP+F  
Sbjct: 142 GQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGISGIILLHPYFWS 201

Query: 65  ----DGGENRQ----------WKYLCP-ATSGDDDPRL---KPAAEDLAKLPCGRVKIFL 124
               D  + +           W    P +  G DDP L   +  + DL+ L CG+V + +
Sbjct: 202 KTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQSESVDLSGLGCGKVLVMV 261

Query: 125 AEEDFLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
           AE+D L      Y   L++ GW G VE VE  GEDHVFHL KP+C+NA ++  K + FI
Sbjct: 262 AEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNAIEVMHKFSGFI 320

[Close]

BLAST of Spg000520 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.5e-27
Identity = 66/175 (37.71%), Postives = 90/175 (51.43%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           + R GPE WLN+HADF++V LAGDSAGANI +++A R           K+  M L HP+F
Sbjct: 139 ITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMILFHPYF 198

Query: 61  GDGG------------ENRQWKYLCP-ATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEED 120
                             R W+   P + +G +DP +     DL  L C RV + +A  D
Sbjct: 199 LSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTGLGCRRVLVMVAGND 258

Query: 121 FLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
            L  G   Y  +L++ GW G V+ +E   E HVFHL+ PD ENA  +    A F+
Sbjct: 259 VLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARRVLRNFAEFL 313

[Close]

BLAST of Spg000520 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.5e-27
Identity = 69/175 (39.43%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 5   GPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF---- 64
           GPE W+N+H DF +V LAGDSAG NI ++L  RA    E+L    +  + LIHP+F    
Sbjct: 139 GPETWINKHGDFGKVFLAGDSAGGNISHHLTMRA--KKEKLCDSLISGIILIHPYFWSKT 198

Query: 65  -------GDGGENR----QWKYLCP-ATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDF 124
                   D G+ +     W+   P +  G DDP L     D + L CGRV + +A +D 
Sbjct: 199 PIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSGLGCGRVLVMVAGDDL 258

Query: 125 LKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFIS 164
                  Y E LK+ GW G VE +E   E HVFHLK P+ +NA  + +KL  FI+
Sbjct: 259 FVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQVVKKLEEFIN 311

[Close]

BLAST of Spg000520 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.1e-24
Identity = 63/180 (35.00%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 2   NRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHP-FF 61
           N  GP  W+N+HADF +V L GDSAG NI +++A +AG   E+   +K+  +A++HP F+
Sbjct: 140 NGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--EKKLDLKIKGIAVVHPAFW 199

Query: 62  GD--------------GGENRQWKYLCPATS--GDDDP--RLKPAAEDLAKLPCGRVKIF 121
           G                G    W+ +    S  G DDP   +  +  D + L C +V + 
Sbjct: 200 GTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGSDFSGLGCDKVLVA 259

Query: 122 LAEEDFLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
           +A +D      + Y   L++C W G VE VE  GEDHVFHL+ P  + A    +K   FI
Sbjct: 260 VAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSDKALKFLKKFVEFI 317

[Close]

BLAST of Spg000520 vs. ExPASy TrEMBL
Match: A0A0A0LNZ1 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.7e-65
Identity = 121/164 (73.78%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDG EPWLN + DFNR+CLAGDSAGANIC+YLA+RA SSAE+LGG K+VAMALIHPFF
Sbjct: 144 VNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAEELGGAKVVAMALIHPFF 203

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDGGENR WKYLC  T       L+P  EDLAKL C RVKIFLAE DFLK G   Y+EDL
Sbjct: 204 GDGGENRLWKYLCSETK-----LLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDL 263

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GWNG VETVEHG E+HVFHLKKP+CE A DL EKLASFI+L
Sbjct: 264 KSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFINL 302

[Close]

BLAST of Spg000520 vs. ExPASy TrEMBL
Match: A0A6J1J7Y5 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-64
Identity = 121/164 (73.78%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDGPEPWLNEHAD +R+CLAGDSAGANIC++LA RAGSSAEQLG VK+VA+ALIHPFF
Sbjct: 141 VNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGSSAEQLGRVKVVALALIHPFF 200

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG E+R WKYLC      D   L+P A+DLA LPC RVKIFLAE+D LK G   YDEDL
Sbjct: 201 GDGAEDRLWKYLC-----SDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDL 260

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GW GAVETVEHGGEDHVFHLK P+CE A DL EKLA FI+L
Sbjct: 261 KSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINL 299

[Close]

BLAST of Spg000520 vs. ExPASy TrEMBL
Match: A0A5D3CP06 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00380 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.0e-63
Identity = 117/164 (71.34%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDG EPWLN + DFNR+CLAGDSAGANIC+YLA+RA SS+E+LGG K+VAMALIHPFF
Sbjct: 144 VNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSSEELGGAKVVAMALIHPFF 203

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG E+R WKYLC  T       ++P  E+LAK+ C RVKIFLAE DFLK G   YDEDL
Sbjct: 204 GDGREDRLWKYLCSETR-----LVRPTKEELAKMGCKRVKIFLAENDFLKFGGRNYDEDL 263

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           KR GWNG VETVEHGGE+HVFHLK P+ ENA DL EKLASFI+L
Sbjct: 264 KRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFINL 302

[Close]

BLAST of Spg000520 vs. ExPASy TrEMBL
Match: A0A1S3C9R7 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.0e-63
Identity = 117/164 (71.34%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDG EPWLN + DFNR+CLAGDSAGANIC+YLA+RA SS+E+LGG K+VAMALIHPFF
Sbjct: 144 VNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSSEELGGAKVVAMALIHPFF 203

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG E+R WKYLC  T       ++P  E+LAK+ C RVKIFLAE DFLK G   YDEDL
Sbjct: 204 GDGREDRLWKYLCSETR-----LVRPTKEELAKMGCKRVKIFLAENDFLKFGGRNYDEDL 263

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           KR GWNG VETVEHGGE+HVFHLK P+ ENA DL EKLASFI+L
Sbjct: 264 KRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFINL 302

[Close]

BLAST of Spg000520 vs. ExPASy TrEMBL
Match: A0A6J1FTL3 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 9.1e-63
Identity = 117/164 (71.34%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           +NRDGPEPWLN+HAD +R+CLAGDSAGANIC++LA RAG+SAEQLG VK+VA+ALIHPFF
Sbjct: 141 VNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSAEQLGRVKVVALALIHPFF 200

Query: 61  GDGGENRQWKYLCPATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDFLKVGAMKYDEDL 120
           GDG  +R WKYLC      D   L+P A+DLA LPC RVKIFLAE+D LK G   YDEDL
Sbjct: 201 GDGAGDRLWKYLC-----SDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDL 260

Query: 121 KRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFISL 165
           K  GW GAVETVEHGGEDHVFHLK P+CE A DL EKL+ FI+L
Sbjct: 261 KSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINL 299

[Close]

BLAST of Spg000520 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 5.9e-30
Identity = 69/174 (39.66%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 7   EPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFFGD---- 66
           EPW+N++AD + + L GDSAGANI ++LA RA  S + L   K+  + +IHP+F      
Sbjct: 140 EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTL---KIKGIGMIHPYFWGTQPI 199

Query: 67  GGENRQ----------WKYLCPATSGDDDPRLKPAAE---DLAKLPCGRVKIFLAEEDFL 126
           G E +           W+++CP+  G DDP + P A+   DL  L C RV I +AE+D L
Sbjct: 200 GAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGLGCERVMITVAEKDIL 259

Query: 127 KVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFIS 164
                 Y E L +  W G VE +E   +DHVFH+ +PDC+ A ++   LA FI+
Sbjct: 260 NERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEMVRCLALFIN 310

[Close]

BLAST of Spg000520 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.2e-29
Identity = 71/179 (39.66%), Postives = 99/179 (55.31%), Query Frame = 0


Query: 5   GPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSS--AEQLGGVKLVAMALIHPFFG- 64
           G E WLN+HADF+RV L+GDSAGANI +++A RA     +  L    +  + L+HP+F  
Sbjct: 142 GQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGISGIILLHPYFWS 201

Query: 65  ----DGGENRQ----------WKYLCP-ATSGDDDPRL---KPAAEDLAKLPCGRVKIFL 124
               D  + +           W    P +  G DDP L   +  + DL+ L CG+V + +
Sbjct: 202 KTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQSESVDLSGLGCGKVLVMV 261

Query: 125 AEEDFLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
           AE+D L      Y   L++ GW G VE VE  GEDHVFHL KP+C+NA ++  K + FI
Sbjct: 262 AEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNAIEVMHKFSGFI 320

[Close]

BLAST of Spg000520 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.2e-28
Identity = 66/175 (37.71%), Postives = 90/175 (51.43%), Query Frame = 0


Query: 1   MNRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF 60
           + R GPE WLN+HADF++V LAGDSAGANI +++A R           K+  M L HP+F
Sbjct: 139 ITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMILFHPYF 198

Query: 61  GDGG------------ENRQWKYLCP-ATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEED 120
                             R W+   P + +G +DP +     DL  L C RV + +A  D
Sbjct: 199 LSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTGLGCRRVLVMVAGND 258

Query: 121 FLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
            L  G   Y  +L++ GW G V+ +E   E HVFHL+ PD ENA  +    A F+
Sbjct: 259 VLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARRVLRNFAEFL 313

[Close]

BLAST of Spg000520 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.2e-28
Identity = 69/175 (39.43%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 5   GPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHPFF---- 64
           GPE W+N+H DF +V LAGDSAG NI ++L  RA    E+L    +  + LIHP+F    
Sbjct: 139 GPETWINKHGDFGKVFLAGDSAGGNISHHLTMRA--KKEKLCDSLISGIILIHPYFWSKT 198

Query: 65  -------GDGGENR----QWKYLCP-ATSGDDDPRLKPAAEDLAKLPCGRVKIFLAEEDF 124
                   D G+ +     W+   P +  G DDP L     D + L CGRV + +A +D 
Sbjct: 199 PIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSGLGCGRVLVMVAGDDL 258

Query: 125 LKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFIS 164
                  Y E LK+ GW G VE +E   E HVFHLK P+ +NA  + +KL  FI+
Sbjct: 259 FVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQVVKKLEEFIN 311

[Close]

BLAST of Spg000520 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 112.1 bits (279), Expect = 4.4e-25
Identity = 63/180 (35.00%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 2   NRDGPEPWLNEHADFNRVCLAGDSAGANICNYLASRAGSSAEQLGGVKLVAMALIHP-FF 61
           N  GP  W+N+HADF +V L GDSAG NI +++A +AG   E+   +K+  +A++HP F+
Sbjct: 140 NGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--EKKLDLKIKGIAVVHPAFW 199

Query: 62  GD--------------GGENRQWKYLCPATS--GDDDP--RLKPAAEDLAKLPCGRVKIF 121
           G                G    W+ +    S  G DDP   +  +  D + L C +V + 
Sbjct: 200 GTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGSDFSGLGCDKVLVA 259

Query: 122 LAEEDFLKVGAMKYDEDLKRCGWNGAVETVEHGGEDHVFHLKKPDCENAFDLTEKLASFI 163
           +A +D      + Y   L++C W G VE VE  GEDHVFHL+ P  + A    +K   FI
Sbjct: 260 VAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSDKALKFLKKFVEFI 317

The following BLAST results are available for this feature:

BLAST of Spg000520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145358.2	1.2e-64	73.78	2-hydroxyisoflavanone dehydratase [Cucumis sativus] >KGN61726.1 hypothetical pro...	[more]
XP_022986577.1	2.6e-64	73.78	probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxy...	[more]
XP_023512082.1	7.6e-64	73.17	probable carboxylesterase 12 [Cucurbita pepo subsp. pepo]	[more]
XP_008459211.1	1.4e-62	71.34	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo] >KAA0032058.1 2...	[more]
XP_022944136.1	1.9e-62	71.34	probable carboxylesterase 12 [Cucurbita moschata]	[more]

BLAST of Spg000520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SX78	8.3e-29	39.66	Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1	[more]
Q9SMN0	3.1e-28	39.66	Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1	[more]
Q9LMA7	4.5e-27	37.71	Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1	[more]
Q9ZQ91	4.5e-27	39.43	Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1	[more]
Q9FX94	6.1e-24	35.00	Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1	[more]

BLAST of Spg000520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNZ1	5.7e-65	73.78	Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345...	[more]
A0A6J1J7Y5	1.3e-64	73.78	probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV...	[more]
A0A5D3CP06	7.0e-63	71.34	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C9R7	7.0e-63	71.34	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 P...	[more]
A0A6J1FTL3	9.1e-63	71.34	probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 ...	[more]

BLAST of Spg000520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47480.1	5.9e-30	39.66	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48690.1	2.2e-29	39.66	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	3.2e-28	37.71	alpha/beta-Hydrolases superfamily protein	[more]
AT2G03550.1	3.2e-28	39.43	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	4.4e-25	35.00	carboxyesterase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 3..164 e-value: 4.8E-26 score: 94.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 9..162
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 9..142 e-value: 1.3E-13 score: 51.3
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 2..163
None	No IPR available	PANTHER	PTHR23024:SF514	CARBOXYLESTERASE 12-RELATED	coord: 2..163
IPR033140	Lipase, GDXG, putative serine active site	PROSITE	PS01174	LIPASE_GDXG_SER	coord: 19..31

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000520.1	Spg000520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity