Spg000518 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000518
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGDSL esterase/lipase
Locationscaffold8: 48068796 .. 48074392 (+)
RNA-Seq ExpressionSpg000518
SyntenySpg000518
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTGAATGCCAGAAGACTGCCGGGTTTTATAAGGATTCGAGAGTTATTGCGAAAAATTATTGTGAGAAACCAACTGCTGGATCAAGTTATTTCAACCTCAAAGGCAAAACAAAGGGAATTGAATCTGACAAATTGGAATTTGACTTGGAAAAGGGAAATACTACTTCACTCACTTCGCAACAATTAGTCTGTAAAAATGTTTCCATTGGTTGTCGGTCAATTACCGACCTTCCTCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCTAAGGAGCTTGGCATTGTATCTTGTGTGTCAACGATTCTTCATAGGCTAGCATCTGAACATCATGTGTGGAAGGAATTCTACAGCGAGAGATGGGGACTTCCAGTTTCGGCTTCGGCTGTGTCCGCACCCGTGGCTGCTACTGGATTTTCCGATGAGAAGTCATGGAAAGATCTTTTTGTCGAGAGAGAGTTTAGGAGTAAAACTTTTATGGGACGATACAGTATGGAGGTTTTGCATGGTCATACTGAAGCAATTCGTACTGTTTTTCTTCTGGCTTCTGCAAAACTTATATTTACTTCTGGATATGACTCAATTGTGAGAATCTGGGACTTGGAAGAAGGATTGTCCATTGCTTCATCACGATCGCTTGGTTGCACCATTCGTGCACTTGCAGCCGATACAAAATTGTTGGTTGCTGGAGGCACTGATGGCTTTATCCATTGTTGGAAGGTAACAGAGGGGGTTCCACACTTGTTTGATGTGAAAGGCCCTCAAAATCACAATACCGAGTTCCGGCTTTGGGAGCATGAAGGACCCATAACCTCACTTGCTCTGGATCTAGCTAGGATTTATAGTGGTTCTTGGGACATGACTGTCCGAGTATGGGATCGATTTTCACAGAATTGCTTAAGGATTTTGAGGCATGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACTGTTGCCAGCGCATCAGGCTCAGATGTATACGTCTGGGATACTAATAGTGGCGAATTGGCTACTGTCATTCATGATGCTCACGTTGGCAAGGCTTATGCCCTGGCACGAAGCCATACAGGCGATTTTCTATTTACTGGAGGGGAAGATGGTGCAATTCACATGTTTGATATTACTAATCGTCATTTTGATACAAGCGCTCAACTTGTTGGATCTTGGATTCCTCATTCAGGTCCAGTTTATTCTCTTGCATTTGAGTTTCCATGGTTGGTTTCAGCTTCAAGTGACGGGAAACTCTCACTCATCGATGTGAGAGCACTGCTTAGGACGAGAAAACGAACTCTGTGGAAGAGAGGTTCTGAGGGACAACATGTGAGTATCAGTGTGGAGCCTCCACAGAGAATGTTGCACGGATTTGGGAACAATTTGTTTGGCGTGGACATTGGTGCGGATCGTATTGTTTGTGGAGGAGAGGAAGGTGTTGTGAGGATCTGGAACTTCTCGCAGGCTCTGGAAACTGAACGGAGGGCTCGTGCTCTAAGAGGAATTAGGTTAGAGAATAGAATGAGACGGCGTAGGCTTCAAATAGAAATGAACACGAAAGGTGGTGGTGGTAGGAGTGATCAGTGCTCAGTTGCAGCCAAGAAAACACCGATGAACAGCGATCGGGTTAATGTTTGGCACAACAAACGTGGGGTGAGTGGGAAGCTCAAAGCTTAGGTGATAGAATCATTTGATTATCTGTTTCCTCAATTTGAATTTGTTGTCATGATTAAGTAAGCTGGCAGCAGCGAATTGTAGATGTTGATTCTGTATCTCAATTTCAAGCACCGGCTAATTGCTGATTCTGTTCCCTTCCCATAAGCTTCTCGAATTCTAGTTTGGCTCGCCCATGTTGCTTTATTTGTATGTGATAGACGTTATAGAAAACCTCAAAAAGATGAAAGCTTAAAGAACATTCCTCAAACAATCTGATTTCAGGCTTGGAATATGGATGTCCTTTTAGCTCTCTTTTTTTGTTGGGTGGTGAACTGTTGATTACTTTTTGGCATTTATGATTTGTTACTAAAAATATGTAAAAAGAGAACAAGCAAGAAATTTGGCAAAGCAACTGGCCAAGACTTTCTTTAAAGATTACAGTTTTAAATACTAAGAAAAGAAAAGGAAAATGGAATTTTAAAAAACTCTAAATACATGAAAAGGTTAAATAAACAGAAAGCAAGGAGGAACGGGGTATTATAAACGTGACAGAGTAGGGAATGGTATTTTTTTCAAACGAAAAAGAAAATTTATAATGAATAGAATTCTTGAGAAGGGATATTTAAAGAGGAGCAAGAATTTGAAAGAGAAATAAAGTAGCATACCATTCGCATTGCTACTAACAGTAAAACACCAAACCTTGTTCTACTTGTAATGGTAGAATCGGTGAGTTGACAGTTGAGAAACAGAAAAGGTGTAAAGCAGAAACTCAAGATTCAGAAATTCAGCCTGTGGAGAATGGAGATGGAGTTCTTGTTCTTGATCGCTGTGTTATTGGGGTTTGCTGCTGCCGAAGCTCAAGGATCTAACGTCAGCGCCATGTTCTTGTTGGGAGATTCTTCTGTGGATTGTGGCTTAAACACTCTGTTTTACCCTCTTCTTCACCACAATTTCTCCCTCCTTCCTTGTGACGCCAACAACTACACTACTCTTCTTCCTCATCTTCTTGGTTAGTACTCTATTTTCTTCCATCTTTGTTTCTTTCTTGTGTAAATCTTGGTTTCTACTCTCTTTTGAACAATGCCATGCTTGTTTTATCTTGTGAAATCTTCGACTCCTAAAATGCAATCTCAACCCATGTATTCTTGCAGCTGAGAAGATAGGCGTACCACAGTCTCAACCCATGTACAATCAGAATGGTTCCATTGAAGCCATCCTAAACGGCCTCAGCTTTGGTGCCCCACAGGCAACCATCATGAGCAGCGCCCAAAGCTATCAGTCTCTCAACCAACAGCTCCGCCAAGTACTTGACACCATTCAGCTCTTGCGCCTGCAACTCGGACTAGGCGCCGCTCGCCATTTCATCCAAACATCGCTCTTCTACCTCTCATTCGGCGAGGTCGACTTCATCAATCTCTACCTTCTGAATTCGACATCCAAAAGTTTGAAGTACGAGGGCGAGGAATTCGCTCGGCTGCTGGTATCCCAAATGGTGATCGCTATAAGAAACCTAGAGGAAGCAGGCGCGAGGAAGATCGTATGCATGGGAATACTGCCATTGGGATGCAGTCCTCGAGTCCTATCTCAATGGCGCGATTCCCCTGCGGCTATATACGACGAAAAGGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAAGTACAACCAGGTGATGGAGAAGCAGATCGTGAAGCTGAACGCACAGTTTGGTGATGGTCGAATGGTTTTTTGCGACGTGTTCAAAGGGATGATGGAGATCATCGGCAACCCGAGGAAATACGGTATGTGTAAGAAGAAGACGAAGAAGAAAGATTGTTAATGTTGTATGATTAGATTGAAGATTTGAATGGGAAATGTGTGCAGGATTCTCGGAGGCAAGGAGCGCATGCTGTGGGCTTGGTTTGTACAATGCAAGCATAGGATGCGTGGCAATGGAGATAGCATGCAGCCAAAGCCAAGTTTCAAGGCATGTGTGGTGGGATCTCTACAATCCGACGGCGGCTGTCAATTCCTTGCTTGCGGATTCGGCATGGCGTAATCACCCCTTCTCCACCCTCTGCCTTCCCTCCACCCTTCAAGACTTGCTTAAATGATTTACTCATTCAAATACTTTTAGTTCAGAACGAACTAGACCAATCCTTCCTTTTGCACAAGGTTGTTTCAAATGAAACGGAACGATAATGGAGAGGATTTTTCTGAAAAGTTTTGTAAAATCGTGCAGAATGCTCTCTTCCCTTTTTTGATTCCTTTTGCTTTCACTTGGGATATATGTCCAATTTAGGCTTTTGTGTAAAATAAGATGAAAGTGGACATTTATAAAATATAATGTCACACTTGTTGAAATAAACAACTTCAGGGTTAGATTATATTAATTATTAAACAAAGCAGTTAGGCATTCCAAACCTAAATCTAAATCCTTTTGCTTTCATCTCATCTCAAGACCAAGCAGAGAACACTCCCTTCAATGGCTGCTGCTGCTGCATCACCCCACTTCCTCCACGTTTTCTCTTCTTTATCTCTAACACGCGCTCCATCTTCACGTTCCCGTTCTCTTGTGATCGCCTCCTCTAAATCCGACAATGCCCCTCTCGTCGGCCGCAAGCTCCGAGCAGCCGTCATCGGCGGTGGCCCGGCTGGATCTTCCGCCGCGGAAGCTCTGGCAGCTGGTGGCGTCGAGACCTACCTATTCGAGCGCAGCCCAGCGTCCGCCAAGCCCTGCGGCGGCGCCCTCCCTCTCTGCATGCTTAGCGAGTTCGATATCCCTGCTCATCTCATCGATCGCCATGTCACTCGTATGAAAATCATCTCCCCTTCAAATCTCGCCGTCGACTTCGGAAAAACCTTGAAGTCCCATGAGTACATACCCATGCTACGCCGCGAAGTGCTCGATTCCTTTCTCCGCTCCCGCGCCCAGTCCAACGGCGCCAATCTCATCCACGGACTCGTGCTGAATCTCGAGGTCCCTGCCTTCTCCAACGCACCGTACGTTGTGCATTACACGGCCGACAACTCACGGCGATCCTTGGCCGTGGATGTGGTGATCGGCGCGGACGGCGCCAACAGCCGAGTAGCGAAGTCGATAGGCGCTGGAGATTACTCGTGCGCGATGGCTTTCCAAGAGAGAATCAAATTACCAGAGGAGAAAATGAAGTACTACGAGAATCTGGCGGAAATGTACGTGGGAAACGACGTATCGCCGGACTTCTACGCGTGGGTATTTCCAAAGTGCGACCATGTGGCGGTGGGTACAGGGACGGTATGCTCGAGGCAAGACATAAAGGTGTACCAGAGAGGAATCCGGGAGAGGGCAAAGAGTAAGATCGGCGGGGGGAAGGTGATAAAAGTAGAGGCACATCCGATACCGGAGCACCCACGAGCGAGGCGAGTGAGAGGGCGGGTGGCGCTGGTGGGGGACGCGGCCGGGTACGTGACGAAGTGCTCCGGCGAGGGCATATACTTTGCGGCGAAGTCAGGGAGGATGTGTGGGGAGGCAGTGGTGAGAGCGTCGGAAGGAGGGGAGAGGATGATCGGAGAAAGGGATCTGAAGAGAGAGTATCTGAGAGAGTGGGACGGCAAGTACGCGGCGACGTTCAGGTTCCTGGATGTGTTGCAGAGAGTGTTCTATGGAGACAACGCTGGAAGGGAAGCATTGGTGGAGATGTGTGGGAATGAGTATGTGCAGAGAATGACGTTCGAAAGCTATTTGTACAAGAAATTGGCAGAGGGAAACAGATGGGAAGATGCAAAGTTGATGTGGAACACCATTGCAAGTCTCGTCAAGTGTGGGATCGTTGGGAGTGAAAGGATTGCTTTCCTCTAGTTTCTTTGTACTTGTACTTGTAGCCCCTTCCGCGCCTTTACCCTTTATGTGAACGAACTCATTTGTATCATCATCATATCATCATCACATTTTAATCAAAACAAAGTTCAATTCCAAG

mRNA sequence

ATGGCATTTGAATGCCAGAAGACTGCCGGGTTTTATAAGGATTCGAGAGTTATTGCGAAAAATTATTGTGAGAAACCAACTGCTGGATCAAGTTATTTCAACCTCAAAGGCAAAACAAAGGGAATTGAATCTGACAAATTGGAATTTGACTTGGAAAAGGGAAATACTACTTCACTCACTTCGCAACAATTAGTCTGTAAAAATGTTTCCATTGGTTGTCGGTCAATTACCGACCTTCCTCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCTAAGGAGCTTGGCATTGTATCTTGTGTGTCAACGATTCTTCATAGGCTAGCATCTGAACATCATGTGTGGAAGGAATTCTACAGCGAGAGATGGGGACTTCCAGTTTCGGCTTCGGCTGTGTCCGCACCCGTGGCTGCTACTGGATTTTCCGATGAGAAGTCATGGAAAGATCTTTTTCATGAAGGACCCATAACCTCACTTGCTCTGGATCTAGCTAGGATTTATAGTGGTTCTTGGGACATGACTGTCCGAGTATGGGATCGATTTTCACAGAATTGCTTAAGGATTTTGAGGCATGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACTGTTGCCAGCGCATCAGGCTCAGATGTATACGTCTGGGATACTAATAGTGGCGAATTGGCTACTTTGAGAAACAGAAAAGGTGTAAAGCAGAAACTCAAGATTCAGAAATTCAGCCTGTGGAGAATGGAGATGGAGTTCTTGTTCTTGATCGCTGTGTTATTGGGGTTTGCTGCTGCCGAAGCTCAAGGATCTAACGTCAGCGCCATGTTCTTGTTGGGAGATTCTTCTGTGGATTGTGGCTTAAACACTCTGTTTTACCCTCTTCTTCACCACAATTTCTCCCTCCTTCCTTGTGACGCCAACAACTACACTACTCTTCTTCCTCATCTTCTTGCTGAGAAGATAGGCGTACCACAGTCTCAACCCATGTACAATCAGAATGGTTCCATTGAAGCCATCCTAAACGGCCTCAGCTTTGGTGCCCCACAGGCAACCATCATGAGCAGCGCCCAAAGCTATCAGTCTCTCAACCAACAGCTCCGCCAAGTACTTGACACCATTCAGCTCTTGCGCCTGCAACTCGGACTAGGCGCCGCTCGCCATTTCATCCAAACATCGCTCTTCTACCTCTCATTCGGCGAGGTCGACTTCATCAATCTCTACCTTCTGAATTCGACATCCAAAAGTTTGAAGTACGAGGGCGAGGAATTCGCTCGGCTGCTGGTATCCCAAATGGTGATCGCTATAAGAAACCTAGAGGAAGCAGGCGCGAGGAAGATCGTATGCATGGGAATACTGCCATTGGGATGCAGTCCTCGAGTCCTATCTCAATGGCGCGATTCCCCTGCGGCTATATACGACGAAAAGGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAAGTACAACCAGGTGATGGAGAAGCAGATCGTGAAGCTGAACGCACAGTTTGGTGATGGTCGAATGGTTTTTTGCGACGTGTTCAAAGGGATGATGGAGATCATCGGCAACCCGAGGAAATACGGATTCTCGGAGGCAAGGAGCGCATGCTGTGGGCTTGGTTTGTACAATGCAAGCATAGGATGCGTGGCAATGGAGATAGCATGCAGCCAAAGCCAAGTTTCAAGGCATGTGTGGTGGGATCTCTACAATCCGACGGCGGCTGTCAATTCCTTGCTTGCGGATTCGGCATGGCGTAATCACCCCTTCTCCACCCTCTGCCTTCCCTCCACCCTTCAAGACTTGCTTAAATGA

Coding sequence (CDS)

ATGGCATTTGAATGCCAGAAGACTGCCGGGTTTTATAAGGATTCGAGAGTTATTGCGAAAAATTATTGTGAGAAACCAACTGCTGGATCAAGTTATTTCAACCTCAAAGGCAAAACAAAGGGAATTGAATCTGACAAATTGGAATTTGACTTGGAAAAGGGAAATACTACTTCACTCACTTCGCAACAATTAGTCTGTAAAAATGTTTCCATTGGTTGTCGGTCAATTACCGACCTTCCTCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCTAAGGAGCTTGGCATTGTATCTTGTGTGTCAACGATTCTTCATAGGCTAGCATCTGAACATCATGTGTGGAAGGAATTCTACAGCGAGAGATGGGGACTTCCAGTTTCGGCTTCGGCTGTGTCCGCACCCGTGGCTGCTACTGGATTTTCCGATGAGAAGTCATGGAAAGATCTTTTTCATGAAGGACCCATAACCTCACTTGCTCTGGATCTAGCTAGGATTTATAGTGGTTCTTGGGACATGACTGTCCGAGTATGGGATCGATTTTCACAGAATTGCTTAAGGATTTTGAGGCATGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACTGTTGCCAGCGCATCAGGCTCAGATGTATACGTCTGGGATACTAATAGTGGCGAATTGGCTACTTTGAGAAACAGAAAAGGTGTAAAGCAGAAACTCAAGATTCAGAAATTCAGCCTGTGGAGAATGGAGATGGAGTTCTTGTTCTTGATCGCTGTGTTATTGGGGTTTGCTGCTGCCGAAGCTCAAGGATCTAACGTCAGCGCCATGTTCTTGTTGGGAGATTCTTCTGTGGATTGTGGCTTAAACACTCTGTTTTACCCTCTTCTTCACCACAATTTCTCCCTCCTTCCTTGTGACGCCAACAACTACACTACTCTTCTTCCTCATCTTCTTGCTGAGAAGATAGGCGTACCACAGTCTCAACCCATGTACAATCAGAATGGTTCCATTGAAGCCATCCTAAACGGCCTCAGCTTTGGTGCCCCACAGGCAACCATCATGAGCAGCGCCCAAAGCTATCAGTCTCTCAACCAACAGCTCCGCCAAGTACTTGACACCATTCAGCTCTTGCGCCTGCAACTCGGACTAGGCGCCGCTCGCCATTTCATCCAAACATCGCTCTTCTACCTCTCATTCGGCGAGGTCGACTTCATCAATCTCTACCTTCTGAATTCGACATCCAAAAGTTTGAAGTACGAGGGCGAGGAATTCGCTCGGCTGCTGGTATCCCAAATGGTGATCGCTATAAGAAACCTAGAGGAAGCAGGCGCGAGGAAGATCGTATGCATGGGAATACTGCCATTGGGATGCAGTCCTCGAGTCCTATCTCAATGGCGCGATTCCCCTGCGGCTATATACGACGAAAAGGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAAGTACAACCAGGTGATGGAGAAGCAGATCGTGAAGCTGAACGCACAGTTTGGTGATGGTCGAATGGTTTTTTGCGACGTGTTCAAAGGGATGATGGAGATCATCGGCAACCCGAGGAAATACGGATTCTCGGAGGCAAGGAGCGCATGCTGTGGGCTTGGTTTGTACAATGCAAGCATAGGATGCGTGGCAATGGAGATAGCATGCAGCCAAAGCCAAGTTTCAAGGCATGTGTGGTGGGATCTCTACAATCCGACGGCGGCTGTCAATTCCTTGCTTGCGGATTCGGCATGGCGTAATCACCCCTTCTCCACCCTCTGCCTTCCCTCCACCCTTCAAGACTTGCTTAAATGA

Protein sequence

MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLTSQQLVCKNVSIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHEGPITSLALDLARIYSGSWDMTVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATLRNRKGVKQKLKIQKFSLWRMEMEFLFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMSSAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCLPSTLQDLLK
Homology
BLAST of Spg000518 vs. NCBI nr
Match: GAY55544.1 (hypothetical protein CUMW_164980 [Citrus unshiu])

HSP 1 Score: 630.2 bits (1624), Expect = 1.9e-176
Identity = 326/615 (53.01%), Postives = 443/615 (72.03%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHE----GPITSLALDLARIYSGSWDM 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+LF E     PITSLALDL RIYSGSWDM
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKELFVEREFRRPITSLALDLTRIYSGSWDM 180

Query: 181 TVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATL----RNR 240
           TVRVWDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT  G L TL    R+ 
Sbjct: 181 TVRVWDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDTIGGCLLTLLRSGRHT 240

Query: 241 KGVKQKLKIQKFSLWRMEMEF---LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLN 300
            G +    I+     R++ +     + I   L F  + A+G+NV++  +LGDSSVDCG N
Sbjct: 241 SGKRVSRVIEVEPPQRIQADHDAPCYAIQNALHF-PSYARGNNVTSFNVLGDSSVDCGEN 300

Query: 301 TLFYPLLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAP 360
           TLFYP+LHHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ 
Sbjct: 301 TLFYPILHHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSA 360

Query: 361 QATIMS-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLY 420
           QATIM+ S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+
Sbjct: 361 QATIMNPSSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLF 420

Query: 421 LLNSTSKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDS 480
           L +S+    KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S
Sbjct: 421 LQSSSGVMGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNS 480

Query: 481 PAAIYDEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKY 540
            A   + KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ Y
Sbjct: 481 TAGDDEGKGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYY 540

Query: 541 GFSEARSACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNH 600
           GF + ++ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   
Sbjct: 541 GFEDPKTACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGR 593

Query: 601 PFSTLCLPSTLQDLL 602
           P   +C P +++ L+
Sbjct: 601 PLFDICRPISVRALV 593

BLAST of Spg000518 vs. NCBI nr
Match: GAY55543.1 (hypothetical protein CUMW_164970 [Citrus unshiu])

HSP 1 Score: 619.0 bits (1595), Expect = 4.3e-173
Identity = 316/608 (51.97%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHE----GPITSLALDLARIYSGSWDM 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+LF E     PITSLALDL RIYSGSWDM
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKELFVEREFRRPITSLALDLTRIYSGSWDM 180

Query: 181 TVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATLRNRKGVK 240
           TVRVWDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT    +     +  ++
Sbjct: 181 TVRVWDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDT----IVVKERQAYIR 240

Query: 241 QKLKIQKFSLWRMEMEFLFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFYPLL 300
           ++                       GF     +G+NV++  +LGDSSVDCG NTLFYP+L
Sbjct: 241 EE-----------------------GF-----KGNNVTSFNVLGDSSVDCGENTLFYPIL 300

Query: 301 HHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMS- 360
           HHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ QATIM+ 
Sbjct: 301 HHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSAQATIMNP 360

Query: 361 SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSK 420
           S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+L +S+  
Sbjct: 361 SSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLFLQSSSGV 420

Query: 421 SLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDE 480
             KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S A   + 
Sbjct: 421 MGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNSTAGDDEG 480

Query: 481 KGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARS 540
           KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ YGF + ++
Sbjct: 481 KGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYYGFEDPKT 540

Query: 541 ACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCL 600
           ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   P   +C 
Sbjct: 541 ACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGRPLFDICR 555

Query: 601 PSTLQDLL 602
           P +++ L+
Sbjct: 601 PISVRALV 555

BLAST of Spg000518 vs. NCBI nr
Match: KAE8037683.1 (hypothetical protein FH972_010250 [Carpinus fangiana])

HSP 1 Score: 607.1 bits (1564), Expect = 1.7e-169
Identity = 343/730 (46.99%), Postives = 444/730 (60.82%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQK  G  K      +  CE+     S FN + KTK   +  L+   +KG  TS  
Sbjct: 1   MAFECQKGTGVSKKLVDCVEISCEEQVE-FSQFNSEQKTK--TTAILDLTSKKGIVTSAN 60

Query: 61  SQQLVCKNVSIGCR-SITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEF 120
           S+ L+  +V    R SITDLPPA+ISEI N LDPKELGIVSCVS  LHR+AS+HHVWK+F
Sbjct: 61  SKHLLANDVLPNFRLSITDLPPALISEIFNSLDPKELGIVSCVSASLHRVASDHHVWKKF 120

Query: 121 YSERWGLPVSASAVSAPVAATGFSDEKSWKDLF--------------------------- 180
           Y ERWGLP     V  P+  +G+SDEKSWKDLF                           
Sbjct: 121 YCERWGLP----TVPTPL-GSGYSDEKSWKDLFVEREFRSRTFMGRYSMDVLGSRSKSTE 180

Query: 181 -----HEGPITSLALDLARIYSGSWDMTVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVA 240
                HEGPITSLALDL R+YSGSWDMTVRVWDRFS  CL++LRH DWVWGLVPHDTTVA
Sbjct: 181 FRLWEHEGPITSLALDLTRMYSGSWDMTVRVWDRFSLKCLKVLRHSDWVWGLVPHDTTVA 240

Query: 241 SASGSDVYVWDTNSGELATLRNRKGVKQKLKIQK-------------------------- 300
           S SGSDVYVWD+ SG L T+ +   V     + +                          
Sbjct: 241 STSGSDVYVWDSGSGTLMTVIHNAHVGNTYSLARSHTGNFLFTGGEDGAIHMFEISSHCA 300

Query: 301 ------FSLW--------RMEMEFLFLIA------------------------------- 360
                  + W         +  EF +L++                               
Sbjct: 301 ETSFFQVATWIPHSGPVHSLAFEFPWLVSASSDGKLSLIDVRKLLRTNRSASGTHVSRAK 360

Query: 361 ------------VLLGFAA------------AEAQGSNVSAMFLLGDSSVDCGLNTLFYP 420
                       +L G  +             E + SN +A+++LGDSSVDC  N+LFYP
Sbjct: 361 HADRNSVEPPQRMLHGHGSNLFSVDIGSETPLEEKVSNATALYILGDSSVDCRDNSLFYP 420

Query: 421 LLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIM 480
           LLH N SL PC+ ++ + LLPHLLAEK+G+    P Y+QNGSIE +  GL+FG+ +ATIM
Sbjct: 421 LLHRNLSLYPCNGSD-SNLLPHLLAEKMGLQNIPPFYSQNGSIEELFLGLNFGSAEATIM 480

Query: 481 S-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNST 540
           + S +SYQSLNQQLRQV +T+QLL+LQ G   A  F+++S  YLS G+ D+I+L+L NS+
Sbjct: 481 NPSRRSYQSLNQQLRQVFETLQLLQLQFGEYGAERFVRSSAVYLSLGKDDYIDLFLRNSS 540

Query: 541 SKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIY 600
              LK+  +EFAR+LV+QMV  IRNL  A  RKI+C+GILPLGC+PR + +WR++ A   
Sbjct: 541 GVRLKFSSQEFARILVNQMVHVIRNLYNANFRKIICVGILPLGCAPRTVWEWRNASAGED 600

Query: 601 DEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEA 602
           D  GCVKE+NE+V++YN +++ QIV+LN++  D ++VFCDV++G+M+I+ NPR YGF + 
Sbjct: 601 DGGGCVKEINEMVLEYNGMLDDQIVELNSKLPDVQIVFCDVYQGIMKILANPRLYGFEDR 660

BLAST of Spg000518 vs. NCBI nr
Match: GAY55542.1 (hypothetical protein CUMW_164970 [Citrus unshiu])

HSP 1 Score: 597.4 bits (1539), Expect = 1.3e-166
Identity = 311/611 (50.90%), Postives = 428/611 (70.05%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHEGPITSLALDLARIYSGSWDMTVRV 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+L                  GSWDMTVRV
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKEL------------------GSWDMTVRV 180

Query: 181 WDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATL----RNRKGVK 240
           WDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT  G L TL    R+  G +
Sbjct: 181 WDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDTIGGCLLTLLRSGRHTSGKR 240

Query: 241 QKLKIQKFSLWRMEMEF---LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFY 300
               I+     R++ +     + I   L F  + A+G+NV++  +LGDSSVDCG NTLFY
Sbjct: 241 VSRVIEVEPPQRIQADHDAPCYAIQNALHF-PSYARGNNVTSFNVLGDSSVDCGENTLFY 300

Query: 301 PLLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATI 360
           P+LHHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ QATI
Sbjct: 301 PILHHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSAQATI 360

Query: 361 MS-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNS 420
           M+ S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+L +S
Sbjct: 361 MNPSSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLFLQSS 420

Query: 421 TSKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAI 480
           +    KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S A  
Sbjct: 421 SGVMGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNSTAGD 480

Query: 481 YDEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSE 540
            + KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ YGF +
Sbjct: 481 DEGKGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYYGFED 540

Query: 541 ARSACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFST 600
            ++ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   P   
Sbjct: 541 PKTACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGRPLFD 571

Query: 601 LCLPSTLQDLL 602
           +C P +++ L+
Sbjct: 601 ICRPISVRALV 571

BLAST of Spg000518 vs. NCBI nr
Match: KAF5744955.1 (hypothetical protein HS088_TW07G00536 [Tripterygium wilfordii])

HSP 1 Score: 573.5 bits (1477), Expect = 2.1e-159
Identity = 327/716 (45.67%), Postives = 435/716 (60.75%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     K+      + CEK +          + + I    L+   +    +S T
Sbjct: 1   MAFECQECTEVSKN-----LDNCEKDSCLEQI-----EAQHINISTLDCHHKNCIKSSYT 60

Query: 61  SQQLVCKNVSIGCR-SITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEF 120
           ++Q  C NV  GCR SITDLPPA+ISEILNCLDPKELG+VSCVS IL+RLASEH VWKEF
Sbjct: 61  AKQSPCNNVLSGCRLSITDLPPALISEILNCLDPKELGVVSCVSPILYRLASEHRVWKEF 120

Query: 121 YSERWGLPVSASAVSAPVAATGFSDEKSWKDLF----HEGPITSLALDLARIYSGSWDMT 180
           YSERWGLP+        V  +  SDEKSWK+LF       P+TSLALDL  IYSGSWDM 
Sbjct: 121 YSERWGLPI--------VPTSMVSDEKSWKELFVQREFRRPVTSLALDLTSIYSGSWDMA 180

Query: 181 VRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSG------------- 240
           VRVWDR S  CL++LRHGDWVW L PHDTTVASASGSDVYVW+T  G             
Sbjct: 181 VRVWDRASLKCLKVLRHGDWVWSLAPHDTTVASASGSDVYVWETKKGIPLGVIHNAHDGN 240

Query: 241 -------------------------------------ELATLRNRKG------------- 300
                                                 +AT     G             
Sbjct: 241 IYSLARSHTGDFIFTGGEDGAIHMFEMTSHCVKADIVRVATWIPHSGPVYSLAFEFPWLV 300

Query: 301 ------------VKQKLKIQKFSLWRMEMEF---------------------LFL--IAV 360
                       V+  L+ ++ SL +  M+F                     LF   I  
Sbjct: 301 SASSDGTMSLIDVRTLLRTRRRSLGKNAMKFKHANPNSLEPPQRMLHGFGANLFSVDIGA 360

Query: 361 LLGFAA---------AEAQGSNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNY 420
            LG A+         +   GS  +A++++GDSSVDCG NTLFYPL+ HN SLLPC  N  
Sbjct: 361 DLGMASVTNGQFSTLSTEGGSYGAALYVMGDSSVDCGDNTLFYPLVRHNLSLLPC--NGS 420

Query: 421 TTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMS-SAQSYQSLNQQLRQ 480
            +LLPHLLA KIG+P     Y+QNGSI+ +L+G+++G+ QATIM  S+QS+QSLNQQLRQ
Sbjct: 421 ASLLPHLLAGKIGLPYIPSFYSQNGSIDGLLSGVNYGSAQATIMKPSSQSHQSLNQQLRQ 480

Query: 481 VLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYEGEEFARLLV 540
           V +T Q+L+LQLG  +A+ FI++S+FYLSFG+ D+I+L+L N++   LKY G EFA +LV
Sbjct: 481 VSETFQILQLQLGDDSAQRFIRSSIFYLSFGKDDYIDLFLRNTSGIMLKYSGPEFAHILV 540

Query: 541 SQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDE--KGCVKEMNELVV 600
           SQMV AI+ L +A  RKI+C+GILPLGC+PR +  W +  A   D+   GCV+E+NEL++
Sbjct: 541 SQMVDAIKYLYDANVRKIICLGILPLGCTPRAVLDWYNITAVGDDDYGSGCVEEINELIL 600

Query: 601 KYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASI 602
           +YN +++++IV++ ++  D +++FCDV++G+MEII N   YGF +  +ACCG+GL  A +
Sbjct: 601 EYNTMLDERIVEMKSELPDAQIIFCDVYQGIMEIITNQNLYGFKDIENACCGVGLRGAMV 660

BLAST of Spg000518 vs. ExPASy Swiss-Prot
Match: Q94AD8 (F-box/WD-40 repeat-containing protein At5g21040 OS=Arabidopsis thaliana OX=3702 GN=At5g21040 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.6e-48
Identity = 127/341 (37.24%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKG---IESDKLEFDLEKGNTT 60
           M FECQ+      D R           +G    +L+    G   I + KL    +KG   
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDGSVEIPNVKLCCQKKKGTLV 60

Query: 61  SLTSQQLVC-KNVSIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVW 120
              S+QL+  K++S    +I DLP A+ISEILNCLDPKELG+VSCVST LHRLASEHH W
Sbjct: 61  PSGSKQLLSDKDLS---TTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLASEHHAW 120

Query: 121 KEFYSERWGLPVSASAVSAPVAATGFSDEKSWKDLF------------------------ 180
           KEFY ERWGLPV   A     A++G SDE+SWKDLF                        
Sbjct: 121 KEFYRERWGLPVVFGA-----ASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHTE 180

Query: 181 ------------------------------------------------------------ 222
                                                                       
Sbjct: 181 AVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGTD 240

BLAST of Spg000518 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 5.7e-35
Identity = 102/331 (30.82%), Postives = 159/331 (48.04%), Query Frame = 0

Query: 269 SNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNYTT-------LLPHLLAEKIG 328
           + V AMF+LGDS VD G N     +   NF     D N   T           LLA  + 
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLE 96

Query: 329 VPQSQPMYNQNGSIEAILNGLSFGAPQATIMS-SAQSYQ---SLNQQLRQVLDTIQLLRL 388
           +P   P  +   S   IL G+++ +  A I+  S  +Y    SLNQQ+  +  T+  LR 
Sbjct: 97  IPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRT 156

Query: 389 QLGLGAARHFIQTSLFYLSFGEVDFINLYLL-NSTSKSLKYEGEEFARLLVSQMVIAIRN 448
            +       ++  SL  L FG  D+IN YL+ N    S+++   +FA LL+SQ    +  
Sbjct: 157 MMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLT 216

Query: 449 LEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCVKEMNELVVKYNQVMEKQIV 508
           L   G RKI   G+ PLGC P   ++    P        CV  +N+++  +NQ ++  + 
Sbjct: 217 LYSLGLRKIFIPGVAPLGCIPNQRARGISPP------DRCVDSVNQILGTFNQGLKSLVD 276

Query: 509 KLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASIGCVAMEIACSQ 568
           +LN +      V+ + +  + +I+ NP  YGFS    ACCG+G     I C+ ++  C  
Sbjct: 277 QLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPN 336

Query: 569 SQVSRHVWWDLYNPTAAVNSLLADSAWRNHP 588
              +++V+WD ++PT   NS+LA  A+   P
Sbjct: 337 R--NQYVFWDAFHPTQTANSILARRAFYGPP 359

BLAST of Spg000518 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 9.4e-30
Identity = 94/362 (25.97%), Postives = 177/362 (48.90%), Query Frame = 0

Query: 252 LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNT-LFYPLLHHNFSLLPCD------ 311
           L L  V+  +A A+   ++    ++ GDS  + G N  L Y L   +F     D      
Sbjct: 6   LALAIVISTYATAQPASTSSLVTYIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKA 65

Query: 312 ANNYTT--LLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMSSAQSY---- 371
              +T    +  +++ K+G+    P  + + + +A L+G+++ +  A I++    Y    
Sbjct: 66  TGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGIYFIQR 125

Query: 372 QSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYE 431
            + N Q+     T +++R ++G GAA   +  +++++  G  D++N +L    +   +Y 
Sbjct: 126 LTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYT 185

Query: 432 GEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSP--RVLSQWRDSPAAIYDEKGC 491
            +EF  LL S +   +  + + GARK++  G+ PLGC P  RV S+ R           C
Sbjct: 186 HDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTR----------MC 245

Query: 492 VKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACC 551
           +  +NE V+++N   +K ++ LN +    +  F D +  ++++I NP  YGF  A ++CC
Sbjct: 246 LNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCC 305

Query: 552 GLGLYNASIG--CVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCLP 597
            +   + S+G  C+     C   Q    V+WD ++P+ + N +LAD     H FS+L   
Sbjct: 306 NV---DTSVGGLCLPNSKMCKNRQ--DFVFWDAFHPSDSANQILAD-----HLFSSLLSS 347

BLAST of Spg000518 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 9.7e-27
Identity = 89/342 (26.02%), Postives = 154/342 (45.03%), Query Frame = 0

Query: 271 VSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNYTT-------LLPHLLAEKIGVP 330
           V A+F+ GDS +D G N         N+     D N   T        +   +A+ +G+P
Sbjct: 53  VPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGLP 112

Query: 331 QSQPMYNQNGSIEAILNGLSFGAPQATIM----SSAQSYQSLNQQLRQVLDTI-QLLRLQ 390
              P Y++  + + +L G+++ +  A I+     +       +QQ+     T+ Q+    
Sbjct: 113 LI-PAYSE-ATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKS 172

Query: 391 LGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYEGEEFARLLVSQMVIAIRNLE 450
            G  A    +  SLF++  G  D++N YL+ +     +Y  ++F  LLV      +  L 
Sbjct: 173 GGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLTRLY 232

Query: 451 EAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCVKEMNELVVKYNQVMEKQIVKL 510
             G RK V  G+  +GC P +L+Q  D          C +E+N+LV+ +N  ++  I  L
Sbjct: 233 NLGGRKFVVAGLGRMGCIPSILAQGNDGK--------CSEEVNQLVLPFNTNVKTMISNL 292

Query: 511 NAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASIGCVAMEIACSQSQ 570
           N    D + ++ D+     +I+ N   YG +     CCG+G     I C+  E  C    
Sbjct: 293 NQNLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNR- 352

Query: 571 VSRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCLPSTLQDL 601
             ++V+WD ++PT  VN ++A  A+      T+  P  +Q L
Sbjct: 353 -DQYVFWDAFHPTEKVNLIMAKKAFAGD--RTVAYPINIQQL 380

BLAST of Spg000518 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.8e-25
Identity = 94/346 (27.17%), Postives = 157/346 (45.38%), Query Frame = 0

Query: 252 LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDAN----- 311
           + L  V LG   +        A F+ GDS VD G N     L   N+     D       
Sbjct: 9   VLLELVWLGNGQSRDHQPLAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFPTGR 68

Query: 312 --NYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMSSAQSY----QS 371
             N  T++ +  A  +G+P   P  +     +  L G+++ +  A I+     +     +
Sbjct: 69  FCNGRTVVDY-GATYLGLPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTT 128

Query: 372 LNQQLRQVLDTIQLLRLQLGLGAA--RHFIQTSLFYLSFGEVDFINLYLL-NSTSKSLKY 431
            N Q+ Q   TI+L   +     A  R ++  S+  ++ G  D+IN YL+    S S  Y
Sbjct: 129 FNGQISQFEITIELRLRRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTY 188

Query: 432 EGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCV 491
            GE++A LL+  +   I  L   GARK+V  G  PLGC P  LS    +     +  GCV
Sbjct: 189 SGEDYADLLIKTLSAQISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCV 248

Query: 492 KEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCG 551
            ++N +V  +N  ++     LN        V+ +VF    +++ NP +YG   +  ACCG
Sbjct: 249 TKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCG 308

Query: 552 LGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAW 584
            G Y  ++ C+ ++  C     +++V+WD ++PT   N ++A + +
Sbjct: 309 NGRYGGALTCLPLQQPCLDR--NQYVFWDAFHPTETANKIIAHNTF 346

BLAST of Spg000518 vs. ExPASy TrEMBL
Match: A0A2H5PT64 (F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164980 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 9.0e-177
Identity = 326/615 (53.01%), Postives = 443/615 (72.03%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHE----GPITSLALDLARIYSGSWDM 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+LF E     PITSLALDL RIYSGSWDM
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKELFVEREFRRPITSLALDLTRIYSGSWDM 180

Query: 181 TVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATL----RNR 240
           TVRVWDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT  G L TL    R+ 
Sbjct: 181 TVRVWDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDTIGGCLLTLLRSGRHT 240

Query: 241 KGVKQKLKIQKFSLWRMEMEF---LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLN 300
            G +    I+     R++ +     + I   L F  + A+G+NV++  +LGDSSVDCG N
Sbjct: 241 SGKRVSRVIEVEPPQRIQADHDAPCYAIQNALHF-PSYARGNNVTSFNVLGDSSVDCGEN 300

Query: 301 TLFYPLLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAP 360
           TLFYP+LHHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ 
Sbjct: 301 TLFYPILHHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSA 360

Query: 361 QATIMS-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLY 420
           QATIM+ S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+
Sbjct: 361 QATIMNPSSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLF 420

Query: 421 LLNSTSKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDS 480
           L +S+    KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S
Sbjct: 421 LQSSSGVMGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNS 480

Query: 481 PAAIYDEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKY 540
            A   + KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ Y
Sbjct: 481 TAGDDEGKGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYY 540

Query: 541 GFSEARSACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNH 600
           GF + ++ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   
Sbjct: 541 GFEDPKTACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGR 593

Query: 601 PFSTLCLPSTLQDLL 602
           P   +C P +++ L+
Sbjct: 601 PLFDICRPISVRALV 593

BLAST of Spg000518 vs. ExPASy TrEMBL
Match: A0A2H5PT71 (F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164970 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 2.1e-173
Identity = 316/608 (51.97%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHE----GPITSLALDLARIYSGSWDM 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+LF E     PITSLALDL RIYSGSWDM
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKELFVEREFRRPITSLALDLTRIYSGSWDM 180

Query: 181 TVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATLRNRKGVK 240
           TVRVWDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT    +     +  ++
Sbjct: 181 TVRVWDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDT----IVVKERQAYIR 240

Query: 241 QKLKIQKFSLWRMEMEFLFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFYPLL 300
           ++                       GF     +G+NV++  +LGDSSVDCG NTLFYP+L
Sbjct: 241 EE-----------------------GF-----KGNNVTSFNVLGDSSVDCGENTLFYPIL 300

Query: 301 HHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMS- 360
           HHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ QATIM+ 
Sbjct: 301 HHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSAQATIMNP 360

Query: 361 SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSK 420
           S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+L +S+  
Sbjct: 361 SSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLFLQSSSGV 420

Query: 421 SLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDE 480
             KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S A   + 
Sbjct: 421 MGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNSTAGDDEG 480

Query: 481 KGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARS 540
           KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ YGF + ++
Sbjct: 481 KGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYYGFEDPKT 540

Query: 541 ACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCL 600
           ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   P   +C 
Sbjct: 541 ACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGRPLFDICR 555

Query: 601 PSTLQDLL 602
           P +++ L+
Sbjct: 601 PISVRALV 555

BLAST of Spg000518 vs. ExPASy TrEMBL
Match: A0A660KMQ4 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_010250 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 8.2e-170
Identity = 343/730 (46.99%), Postives = 444/730 (60.82%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQK  G  K      +  CE+     S FN + KTK   +  L+   +KG  TS  
Sbjct: 1   MAFECQKGTGVSKKLVDCVEISCEEQVE-FSQFNSEQKTK--TTAILDLTSKKGIVTSAN 60

Query: 61  SQQLVCKNVSIGCR-SITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEF 120
           S+ L+  +V    R SITDLPPA+ISEI N LDPKELGIVSCVS  LHR+AS+HHVWK+F
Sbjct: 61  SKHLLANDVLPNFRLSITDLPPALISEIFNSLDPKELGIVSCVSASLHRVASDHHVWKKF 120

Query: 121 YSERWGLPVSASAVSAPVAATGFSDEKSWKDLF--------------------------- 180
           Y ERWGLP     V  P+  +G+SDEKSWKDLF                           
Sbjct: 121 YCERWGLP----TVPTPL-GSGYSDEKSWKDLFVEREFRSRTFMGRYSMDVLGSRSKSTE 180

Query: 181 -----HEGPITSLALDLARIYSGSWDMTVRVWDRFSQNCLRILRHGDWVWGLVPHDTTVA 240
                HEGPITSLALDL R+YSGSWDMTVRVWDRFS  CL++LRH DWVWGLVPHDTTVA
Sbjct: 181 FRLWEHEGPITSLALDLTRMYSGSWDMTVRVWDRFSLKCLKVLRHSDWVWGLVPHDTTVA 240

Query: 241 SASGSDVYVWDTNSGELATLRNRKGVKQKLKIQK-------------------------- 300
           S SGSDVYVWD+ SG L T+ +   V     + +                          
Sbjct: 241 STSGSDVYVWDSGSGTLMTVIHNAHVGNTYSLARSHTGNFLFTGGEDGAIHMFEISSHCA 300

Query: 301 ------FSLW--------RMEMEFLFLIA------------------------------- 360
                  + W         +  EF +L++                               
Sbjct: 301 ETSFFQVATWIPHSGPVHSLAFEFPWLVSASSDGKLSLIDVRKLLRTNRSASGTHVSRAK 360

Query: 361 ------------VLLGFAA------------AEAQGSNVSAMFLLGDSSVDCGLNTLFYP 420
                       +L G  +             E + SN +A+++LGDSSVDC  N+LFYP
Sbjct: 361 HADRNSVEPPQRMLHGHGSNLFSVDIGSETPLEEKVSNATALYILGDSSVDCRDNSLFYP 420

Query: 421 LLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIM 480
           LLH N SL PC+ ++ + LLPHLLAEK+G+    P Y+QNGSIE +  GL+FG+ +ATIM
Sbjct: 421 LLHRNLSLYPCNGSD-SNLLPHLLAEKMGLQNIPPFYSQNGSIEELFLGLNFGSAEATIM 480

Query: 481 S-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNST 540
           + S +SYQSLNQQLRQV +T+QLL+LQ G   A  F+++S  YLS G+ D+I+L+L NS+
Sbjct: 481 NPSRRSYQSLNQQLRQVFETLQLLQLQFGEYGAERFVRSSAVYLSLGKDDYIDLFLRNSS 540

Query: 541 SKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIY 600
              LK+  +EFAR+LV+QMV  IRNL  A  RKI+C+GILPLGC+PR + +WR++ A   
Sbjct: 541 GVRLKFSSQEFARILVNQMVHVIRNLYNANFRKIICVGILPLGCAPRTVWEWRNASAGED 600

Query: 601 DEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEA 602
           D  GCVKE+NE+V++YN +++ QIV+LN++  D ++VFCDV++G+M+I+ NPR YGF + 
Sbjct: 601 DGGGCVKEINEMVLEYNGMLDDQIVELNSKLPDVQIVFCDVYQGIMKILANPRLYGFEDR 660

BLAST of Spg000518 vs. ExPASy TrEMBL
Match: A0A2H5PT73 (F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164970 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 6.5e-167
Identity = 311/611 (50.90%), Postives = 428/611 (70.05%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     KD               S   +L  + +  E + L F++E   +T L 
Sbjct: 1   MAFECQRVTRVSKD------------LTNSVEDSLLSQIES-ELNNLNFEIEATKST-LE 60

Query: 61  SQQLVCKNV--SIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKE 120
           S+QL C +V  ++   +ITDLPPA+ISEILNCLDPKELGIVSCVS ILHRLAS+HH WKE
Sbjct: 61  SKQLPCNDVLSNLHRSTITDLPPALISEILNCLDPKELGIVSCVSPILHRLASDHHAWKE 120

Query: 121 FYSERWGLPVSASAVSAPVAATGFSDEKSWKDLFHEGPITSLALDLARIYSGSWDMTVRV 180
           FY ERWGLP++    SAP+ A GFSD+KSWK+L                  GSWDMTVRV
Sbjct: 121 FYCERWGLPIA----SAPLGA-GFSDDKSWKEL------------------GSWDMTVRV 180

Query: 181 WDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSGELATL----RNRKGVK 240
           WDR    CL++LRH DWV+GL PHDTTVAS SGSDVY+WDT  G L TL    R+  G +
Sbjct: 181 WDRALLKCLKVLRHSDWVYGLAPHDTTVASTSGSDVYIWDTIGGCLLTLLRSGRHTSGKR 240

Query: 241 QKLKIQKFSLWRMEMEF---LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFY 300
               I+     R++ +     + I   L F  + A+G+NV++  +LGDSSVDCG NTLFY
Sbjct: 241 VSRVIEVEPPQRIQADHDAPCYAIQNALHF-PSYARGNNVTSFNVLGDSSVDCGENTLFY 300

Query: 301 PLLHHNFSLLPCDANNYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATI 360
           P+LHHN SL+PC   + +TLLPHLLA+K G+P   P Y+QNGSI  +L+GL++G+ QATI
Sbjct: 301 PILHHNLSLIPCYNGSDSTLLPHLLAKKKGLPYPPPFYSQNGSINGLLSGLNYGSAQATI 360

Query: 361 MS-SAQSYQSLNQQLRQVLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNS 420
           M+ S+QS+QSLNQQLRQV +T QLL+L+LG G+A+  I++S+FYLSFG+ D+++L+L +S
Sbjct: 361 MNPSSQSHQSLNQQLRQVFETFQLLQLELGEGSAKDIIESSVFYLSFGKDDYLDLFLQSS 420

Query: 421 TSKSLKYEGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAI 480
           +    KY G EFA +LV QMV  +R+L +A   +I+CMGILPLGC+PR++ +WR+S A  
Sbjct: 421 SGVMGKYSGLEFASILVDQMVNVMRDLYDANVHRIICMGILPLGCTPRIVWEWRNSTAGD 480

Query: 481 YDEKGCVKEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSE 540
            + KGCV E+NEL+++YN ++E++I+ LN++  +  ++FCD+++G+M+++ NP+ YGF +
Sbjct: 481 DEGKGCVAEVNELILQYNTMLEERIINLNSELPNAHIIFCDIYQGIMQMMNNPQYYGFED 540

Query: 541 ARSACCGLGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAWRNHPFST 600
            ++ACCGLGLY A IGC+++E+AC +   S ++WWDLYNPT AVN+LLADSAW   P   
Sbjct: 541 PKTACCGLGLYGAMIGCLSVEMACERD--SDYIWWDLYNPTKAVNALLADSAWSGRPLFD 571

Query: 601 LCLPSTLQDLL 602
           +C P +++ L+
Sbjct: 601 ICRPISVRALV 571

BLAST of Spg000518 vs. ExPASy TrEMBL
Match: A0A7J7DEZ0 (Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW07G00536 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 1.0e-159
Identity = 327/716 (45.67%), Postives = 435/716 (60.75%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKGIESDKLEFDLEKGNTTSLT 60
           MAFECQ+     K+      + CEK +          + + I    L+   +    +S T
Sbjct: 1   MAFECQECTEVSKN-----LDNCEKDSCLEQI-----EAQHINISTLDCHHKNCIKSSYT 60

Query: 61  SQQLVCKNVSIGCR-SITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEF 120
           ++Q  C NV  GCR SITDLPPA+ISEILNCLDPKELG+VSCVS IL+RLASEH VWKEF
Sbjct: 61  AKQSPCNNVLSGCRLSITDLPPALISEILNCLDPKELGVVSCVSPILYRLASEHRVWKEF 120

Query: 121 YSERWGLPVSASAVSAPVAATGFSDEKSWKDLF----HEGPITSLALDLARIYSGSWDMT 180
           YSERWGLP+        V  +  SDEKSWK+LF       P+TSLALDL  IYSGSWDM 
Sbjct: 121 YSERWGLPI--------VPTSMVSDEKSWKELFVQREFRRPVTSLALDLTSIYSGSWDMA 180

Query: 181 VRVWDRFSQNCLRILRHGDWVWGLVPHDTTVASASGSDVYVWDTNSG------------- 240
           VRVWDR S  CL++LRHGDWVW L PHDTTVASASGSDVYVW+T  G             
Sbjct: 181 VRVWDRASLKCLKVLRHGDWVWSLAPHDTTVASASGSDVYVWETKKGIPLGVIHNAHDGN 240

Query: 241 -------------------------------------ELATLRNRKG------------- 300
                                                 +AT     G             
Sbjct: 241 IYSLARSHTGDFIFTGGEDGAIHMFEMTSHCVKADIVRVATWIPHSGPVYSLAFEFPWLV 300

Query: 301 ------------VKQKLKIQKFSLWRMEMEF---------------------LFL--IAV 360
                       V+  L+ ++ SL +  M+F                     LF   I  
Sbjct: 301 SASSDGTMSLIDVRTLLRTRRRSLGKNAMKFKHANPNSLEPPQRMLHGFGANLFSVDIGA 360

Query: 361 LLGFAA---------AEAQGSNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNY 420
            LG A+         +   GS  +A++++GDSSVDCG NTLFYPL+ HN SLLPC  N  
Sbjct: 361 DLGMASVTNGQFSTLSTEGGSYGAALYVMGDSSVDCGDNTLFYPLVRHNLSLLPC--NGS 420

Query: 421 TTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMS-SAQSYQSLNQQLRQ 480
            +LLPHLLA KIG+P     Y+QNGSI+ +L+G+++G+ QATIM  S+QS+QSLNQQLRQ
Sbjct: 421 ASLLPHLLAGKIGLPYIPSFYSQNGSIDGLLSGVNYGSAQATIMKPSSQSHQSLNQQLRQ 480

Query: 481 VLDTIQLLRLQLGLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYEGEEFARLLV 540
           V +T Q+L+LQLG  +A+ FI++S+FYLSFG+ D+I+L+L N++   LKY G EFA +LV
Sbjct: 481 VSETFQILQLQLGDDSAQRFIRSSIFYLSFGKDDYIDLFLRNTSGIMLKYSGPEFAHILV 540

Query: 541 SQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDE--KGCVKEMNELVV 600
           SQMV AI+ L +A  RKI+C+GILPLGC+PR +  W +  A   D+   GCV+E+NEL++
Sbjct: 541 SQMVDAIKYLYDANVRKIICLGILPLGCTPRAVLDWYNITAVGDDDYGSGCVEEINELIL 600

Query: 601 KYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASI 602
           +YN +++++IV++ ++  D +++FCDV++G+MEII N   YGF +  +ACCG+GL  A +
Sbjct: 601 EYNTMLDERIVEMKSELPDAQIIFCDVYQGIMEIITNQNLYGFKDIENACCGVGLRGAMV 660

BLAST of Spg000518 vs. TAIR 10
Match: AT5G21040.1 (F-box protein 2 )

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-49
Identity = 127/341 (37.24%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKG---IESDKLEFDLEKGNTT 60
           M FECQ+      D R           +G    +L+    G   I + KL    +KG   
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDGSVEIPNVKLCCQKKKGTLV 60

Query: 61  SLTSQQLVC-KNVSIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVW 120
              S+QL+  K++S    +I DLP A+ISEILNCLDPKELG+VSCVST LHRLASEHH W
Sbjct: 61  PSGSKQLLSDKDLS---TTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLASEHHAW 120

Query: 121 KEFYSERWGLPVSASAVSAPVAATGFSDEKSWKDLF------------------------ 180
           KEFY ERWGLPV   A     A++G SDE+SWKDLF                        
Sbjct: 121 KEFYRERWGLPVVFGA-----ASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHTE 180

Query: 181 ------------------------------------------------------------ 222
                                                                       
Sbjct: 181 AVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGTD 240

BLAST of Spg000518 vs. TAIR 10
Match: AT5G21040.2 (F-box protein 2 )

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-49
Identity = 127/341 (37.24%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 1   MAFECQKTAGFYKDSRVIAKNYCEKPTAGSSYFNLKGKTKG---IESDKLEFDLEKGNTT 60
           M FECQ+      D R           +G    +L+    G   I + KL    +KG   
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDGSVEIPNVKLCCQKKKGTLV 60

Query: 61  SLTSQQLVC-KNVSIGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVW 120
              S+QL+  K++S    +I DLP A+ISEILNCLDPKELG+VSCVST LHRLASEHH W
Sbjct: 61  PSGSKQLLSDKDLS---TTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLASEHHAW 120

Query: 121 KEFYSERWGLPVSASAVSAPVAATGFSDEKSWKDLF------------------------ 180
           KEFY ERWGLPV   A     A++G SDE+SWKDLF                        
Sbjct: 121 KEFYRERWGLPVVFGA-----ASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHTE 180

Query: 181 ------------------------------------------------------------ 222
                                                                       
Sbjct: 181 AVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGTD 240

BLAST of Spg000518 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 2.3e-47
Identity = 110/342 (32.16%), Postives = 183/342 (53.51%), Query Frame = 0

Query: 271 VSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNYTTLLP-------HLLAEKIGVP 330
           V A+F+ GDSSVD G N     L   +   LP    ++ T  P        +  + +G+P
Sbjct: 69  VPALFVFGDSSVDSGTNNFLGTLARAD--RLPY-GRDFDTHQPTGRFCNGRIPVDYLGLP 128

Query: 331 QSQPMYNQNGSIEAILNGLSFGAPQATIMSSAQS----YQSLNQQLRQVLDTIQLLRLQL 390
                  Q G++E +  G+++ +  A I+ S+ S      S   Q+ Q +DT Q + L +
Sbjct: 129 FVPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRVSFAMQVEQFVDTFQQMILSI 188

Query: 391 GLGAARHFIQTSLFYLSFGEVDFINLYLLNSTSKSLKYEGEEFARLLVSQMVIAIRNLEE 450
           G  A+   +  S+FY+S G  D+I+ Y+ N ++    Y    F + L S M   ++ L  
Sbjct: 189 GEKASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTPWNFNQFLASNMRQELKTLYN 248

Query: 451 AGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCVKEMNELVVKYNQVMEKQIVKLN 510
              R++V MG+ P+GC+P  + ++R           C +E+N ++++ N VM   + KLN
Sbjct: 249 VKVRRMVVMGLPPIGCAPYYMWKYRSQNGE------CAEEVNSMIMESNFVMRYTVDKLN 308

Query: 511 AQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASIGCVAMEIACSQSQV 570
            +     +++CDVF+  M+I+ N + YGF+E   ACCGLG Y   + C++ E+ACS +  
Sbjct: 309 RELPGASIIYCDVFQSAMDILRNHQHYGFNETTDACCGLGRYKGWLPCISPEMACSDA-- 368

Query: 571 SRHVWWDLYNPTAAVNSLLADSAWRNHPFSTLCLPSTLQDLL 602
           S H+WWD ++PT AVN++LAD+ W N     +C P+ L+ +L
Sbjct: 369 SGHLWWDQFHPTDAVNAILADNVW-NGRHVDMCYPTNLETML 398

BLAST of Spg000518 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 4.0e-36
Identity = 102/331 (30.82%), Postives = 159/331 (48.04%), Query Frame = 0

Query: 269 SNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDANNYTT-------LLPHLLAEKIG 328
           + V AMF+LGDS VD G N     +   NF     D N   T           LLA  + 
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLE 96

Query: 329 VPQSQPMYNQNGSIEAILNGLSFGAPQATIMS-SAQSYQ---SLNQQLRQVLDTIQLLRL 388
           +P   P  +   S   IL G+++ +  A I+  S  +Y    SLNQQ+  +  T+  LR 
Sbjct: 97  IPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRT 156

Query: 389 QLGLGAARHFIQTSLFYLSFGEVDFINLYLL-NSTSKSLKYEGEEFARLLVSQMVIAIRN 448
            +       ++  SL  L FG  D+IN YL+ N    S+++   +FA LL+SQ    +  
Sbjct: 157 MMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLT 216

Query: 449 LEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCVKEMNELVVKYNQVMEKQIV 508
           L   G RKI   G+ PLGC P   ++    P        CV  +N+++  +NQ ++  + 
Sbjct: 217 LYSLGLRKIFIPGVAPLGCIPNQRARGISPP------DRCVDSVNQILGTFNQGLKSLVD 276

Query: 509 KLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCGLGLYNASIGCVAMEIACSQ 568
           +LN +      V+ + +  + +I+ NP  YGFS    ACCG+G     I C+ ++  C  
Sbjct: 277 QLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPN 336

Query: 569 SQVSRHVWWDLYNPTAAVNSLLADSAWRNHP 588
              +++V+WD ++PT   NS+LA  A+   P
Sbjct: 337 R--NQYVFWDAFHPTQTANSILARRAFYGPP 359

BLAST of Spg000518 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 119.0 bits (297), Expect = 1.3e-26
Identity = 94/346 (27.17%), Postives = 157/346 (45.38%), Query Frame = 0

Query: 252 LFLIAVLLGFAAAEAQGSNVSAMFLLGDSSVDCGLNTLFYPLLHHNFSLLPCDAN----- 311
           + L  V LG   +        A F+ GDS VD G N     L   N+     D       
Sbjct: 9   VLLELVWLGNGQSRDHQPLAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFPTGR 68

Query: 312 --NYTTLLPHLLAEKIGVPQSQPMYNQNGSIEAILNGLSFGAPQATIMSSAQSY----QS 371
             N  T++ +  A  +G+P   P  +     +  L G+++ +  A I+     +     +
Sbjct: 69  FCNGRTVVDY-GATYLGLPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTT 128

Query: 372 LNQQLRQVLDTIQLLRLQLGLGAA--RHFIQTSLFYLSFGEVDFINLYLL-NSTSKSLKY 431
            N Q+ Q   TI+L   +     A  R ++  S+  ++ G  D+IN YL+    S S  Y
Sbjct: 129 FNGQISQFEITIELRLRRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTY 188

Query: 432 EGEEFARLLVSQMVIAIRNLEEAGARKIVCMGILPLGCSPRVLSQWRDSPAAIYDEKGCV 491
            GE++A LL+  +   I  L   GARK+V  G  PLGC P  LS    +     +  GCV
Sbjct: 189 SGEDYADLLIKTLSAQISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCV 248

Query: 492 KEMNELVVKYNQVMEKQIVKLNAQFGDGRMVFCDVFKGMMEIIGNPRKYGFSEARSACCG 551
            ++N +V  +N  ++     LN        V+ +VF    +++ NP +YG   +  ACCG
Sbjct: 249 TKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCG 308

Query: 552 LGLYNASIGCVAMEIACSQSQVSRHVWWDLYNPTAAVNSLLADSAW 584
            G Y  ++ C+ ++  C     +++V+WD ++PT   N ++A + +
Sbjct: 309 NGRYGGALTCLPLQQPCLDR--NQYVFWDAFHPTETANKIIAHNTF 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GAY55544.11.9e-17653.01hypothetical protein CUMW_164980 [Citrus unshiu][more]
GAY55543.14.3e-17351.97hypothetical protein CUMW_164970 [Citrus unshiu][more]
KAE8037683.11.7e-16946.99hypothetical protein FH972_010250 [Carpinus fangiana][more]
GAY55542.11.3e-16650.90hypothetical protein CUMW_164970 [Citrus unshiu][more]
KAF5744955.12.1e-15945.67hypothetical protein HS088_TW07G00536 [Tripterygium wilfordii][more]
Match NameE-valueIdentityDescription
Q94AD82.6e-4837.24F-box/WD-40 repeat-containing protein At5g21040 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FVV15.7e-3530.82GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2... [more]
Q9FHQ19.4e-3025.97GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2... [more]
Q9SF789.7e-2726.02GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2... [more]
Q8LFJ91.8e-2527.17GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A2H5PT649.0e-17753.01F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164980 PE=3 SV... [more]
A0A2H5PT712.1e-17351.97F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164970 PE=3 SV... [more]
A0A660KMQ48.2e-17046.99F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_010250 P... [more]
A0A2H5PT736.5e-16750.90F-box domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_164970 PE=3 SV... [more]
A0A7J7DEZ01.0e-15945.67Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW07G00536 ... [more]
Match NameE-valueIdentityDescription
AT5G21040.11.9e-4937.24F-box protein 2 [more]
AT5G21040.21.9e-4937.24F-box protein 2 [more]
AT4G10950.12.3e-4732.16SGNH hydrolase-type esterase superfamily protein [more]
AT1G71250.14.0e-3630.82GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G15720.11.3e-2627.17GDSL-motif lipase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 358..378
NoneNo IPR availableGENE3D1.20.1280.50coord: 64..115
e-value: 7.1E-7
score: 30.9
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 267..601
NoneNo IPR availablePANTHERPTHR45648:SF7OS12G0126100 PROTEINcoord: 267..601
IPR001810F-box domainSMARTSM00256fbox_2coord: 79..119
e-value: 6.1E-7
score: 39.1
IPR001810F-box domainPFAMPF00646F-boxcoord: 75..120
e-value: 8.1E-7
score: 28.7
IPR001810F-box domainPROSITEPS50181FBOXcoord: 73..119
score: 11.246099
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 144..180
e-value: 0.0044
score: 26.3
coord: 183..218
e-value: 0.46
score: 19.1
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 153..180
score: 8.703868
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 277..501
e-value: 1.4E-5
score: 25.7
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 261..586
e-value: 1.3E-46
score: 161.5
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 116..248
e-value: 4.1E-14
score: 53.9
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 71..153
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 141..225

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000518.1Spg000518.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0005515 protein binding