Spg000467 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000467
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAuxin-responsive GH3 family protein
Locationscaffold8: 41316909 .. 41319808 (-)
RNA-Seq ExpressionSpg000467
SyntenySpg000467
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGGAGGCTTCAAACAACTTACCCTCCAAACTTCCTAGCTTTGACATTCATCATTCCCATGAGAAAGATGAACTGGCAATATTGCAGCACATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACAGAAATTCTCTGGCGCAACGCCCATGTCGAGTACCTGCAACAACATGGCCTCAATGGTTGTACCGACAGGAAGACCTTCAAGAAGATGATGCCCGTCGTCTCCTACGAGAATTTAAAATCATACGTGGATCGAATTGCAAATGGCGATACTTCTCCCATACTGTGTTCTGAACCCATATCAGAATTCTTAACCAGGTGAGTATACAATAATCCATTATTGCTATCCACGTTAAGAAAATAAATAAATAAAGCCATTTGATAACCATTTGAAAATACAGTAATCTAATAGCTTAGACCCGTTTGATAACCATTTGGTTTTTAAAAATTAAGCTTATAAATCCTACTTCCACCCTCAAGTTTCTTTGTTTTTCTAATACCTTTATTTCTTTGTTTTTAAAAACCAAGCAAATTTTGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTTTTTTTTGTAGAATTTGGCTAGGAATTCAAATGTAAAACCAAAAATAAAAAACCAAATGGTTATCAAACGGGGTATTAGGCTTTTGAATATGTTTCAAAAAGCAGAAAGAAGTCTTGTGTTTGAATCTAGTGATGTTATTTTCTTCTCAATCAATATTGATAATCACTTGATCATTCGATATCGCGTTTTTTCGGTTTTAGTTCGGGAACATCAGGAGGAGAGAGGAAGTTAATGCCAACAACCGAAGAAGAGCTCAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAGTTCATTCCAGGCTTGGACAAGGGCAAAGGAATGTACTTTTTGTTCGTAAAATCTGAATCCACAACTCCTGGAGGGTTACCTTCCCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACAACGATTACACAAGCCCTAATGAAACAATTCTTTGTACAGACTCATACCAAAGCATGTACTCTCAGTTCCTCTGTGGACTGTGTCTCAGCAGGCAAGTTCTTCGAGTTGGTGCTGTTTTTGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAAGTCATTGGGCCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCTGTGCTTGCTGACTTGATTGAATTTGAGTGCAGCAAAGAAACTTGGGCAGGTATTATAACCAGGCTGTGGCCAAATACCAAGTATGTTGATGTTATTGTGACAGGTACTATGTCACAGTACATCCCAACTTTAGATTACTATAGTAATGGACTCCCTCTTGTTTGTACTATGTATGCTTCTTCTGAGTGCTACTTTGGCATTAACTTAAATCCCTTGTGTAAGCCAAACCAGGTCTCTTACACCCTCATTCCCAACATGGCATATTTTGAATTTTTACCTCTTGATAGGACAAGTGGATTTGGCAGTACTTGTGAATCAGAAGCCATTGAGGAAATAAAGGATGAATTGGTTGATTTGGTGGATGTTGAATTGGGAAAGGAATACGAGCTCGTTATCACCACTTATGCAGGTACGATTTGTACGCACTAATGTGTAGGAGCTCTTATTTACTTTTTGATTTGGTGTATTTTTATTGGGTTATTTCTAATACATTTAATAGATAAAGTCATTAAAGGGTAAAGTCAAATCAAACTCGTCTTTTTATAAGAAAATGAAAACAACTTTCTATCTCTCTCCTAATTGTCAACATTTTAACGATGGTGTGTAAAAACATTCCTACATACCTGGCAAGTGTAAATATTCGTAAAGTGATACCTAGTTGCATAATGGTGCCTAGGGATCTCCCTAAACACTTCATTTATATCTTGACAACTAAAAGAGAGAAACGTAGAAAATGAGAAATATCTTTTAGGTGCATAGGAAGGTACCTACACACCCATATGCAATCAAGGATTGTCCATATCTGTAGACTTTTCTCTTGAAACTCTTCACCATTGCTTGCAACCATGGGTCTTTCCTCACTATGAAAAGCCTTGATTTTTTCTTCAAGAATTTCATTAGGGTTTTTAAGGATTTGATCTTGTTGAAATTTTTTCGATCGATGTGCAGGCAAATATTGTTCTTGGTTTATGCATTTGGGATAATCCATCTTCATCGACATTGCAGGGCTTTATCGGTATCGAGTCGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTCAACTTCGTATGCAGGAAAAACGTCATTCTGAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAGAACGCAGTAATGGAAGCTTCAAAAAACTTAATCCCATACGACACTTGCCTCCTCGAGTACACGAGCTACGCAGATACTCATACAGTTCCAGGCCACTACGTCATATATTGGGAAATTAGCCTAAAGGAAACCACCACCATTCCTCGTTTAGTGTTTGAAAAATGTTGTCTTGAAATCGAAGAATCACTCAACAGCGTGTACCGTCAAGGAAGAGTCTCAGACAAATCAATCGGCCCTTTGGAGATAAAGATTGTTGAGAATGGAACTTTCGACAAACTCATGGACTATGCCATCAATCATGGTGCGTCCATAAACCAATACAAGACCCCTCGCTGTGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTTCGTTGTGTCCAATTATTTCAGCCCAAAATGTCCCAATTGGGTTCCTGGTCGAAAAGAATGGTCTGCCAACAGTTAA

mRNA sequence

ATGCTGGAGGCTTCAAACAACTTACCCTCCAAACTTCCTAGCTTTGACATTCATCATTCCCATGAGAAAGATGAACTGGCAATATTGCAGCACATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACAGAAATTCTCTGGCGCAACGCCCATGTCGAGTACCTGCAACAACATGGCCTCAATGGTTGTACCGACAGGAAGACCTTCAAGAAGATGATGCCCGTCGTCTCCTACGAGAATTTAAAATCATACGTGGATCGAATTGCAAATGGCGATACTTCTCCCATACTGTGTTCTGAACCCATATCAGAATTCTTAACCAGTTCGGGAACATCAGGAGGAGAGAGGAAGTTAATGCCAACAACCGAAGAAGAGCTCAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAGTTCATTCCAGGCTTGGACAAGGGCAAAGGAATGTACTTTTTGTTCGTAAAATCTGAATCCACAACTCCTGGAGGGTTACCTTCCCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACAACGATTACACAAGCCCTAATGAAACAATTCTTTGTACAGACTCATACCAAAGCATGTACTCTCAGTTCCTCTGTGGACTGTGTCTCAGCAGGCAAGTTCTTCGAGTTGGTGCTGTTTTTGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAAGTCATTGGGCCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCTGTGCTTGCTGACTTGATTGAATTTGAGTGCAGCAAAGAAACTTGGGCAGGTATTATAACCAGGCTGTGGCCAAATACCAAGTATGTTGATGTTATTGTGACAGGTACTATGTCACAGTACATCCCAACTTTAGATTACTATAGTAATGGACTCCCTCTTGTTTGTACTATGTATGCTTCTTCTGAGTGCTACTTTGGCATTAACTTAAATCCCTTGTGTAAGCCAAACCAGGTCTCTTACACCCTCATTCCCAACATGGCATATTTTGAATTTTTACCTCTTGATAGGACAAGTGGATTTGGCAGTACTTGTGAATCAGAAGCCATTGAGGAAATAAAGGATGAATTGGTTGATTTGGTGGATGTTGAATTGGGAAAGGAATACGAGCTCGTTATCACCACTTATGCAGGGCTTTATCGGTATCGAGTCGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTCAACTTCGTATGCAGGAAAAACGTCATTCTGAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAGAACGCAGTAATGGAAGCTTCAAAAAACTTAATCCCATACGACACTTGCCTCCTCGAGTACACGAGCTACGCAGATACTCATACAGTTCCAGGCCACTACGTCATATATTGGGAAATTAGCCTAAAGGAAACCACCACCATTCCTCGTTTAGTGTTTGAAAAATGTTGTCTTGAAATCGAAGAATCACTCAACAGCGTGTACCGTCAAGGAAGAGTCTCAGACAAATCAATCGGCCCTTTGGAGATAAAGATTGTTGAGAATGGAACTTTCGACAAACTCATGGACTATGCCATCAATCATGGTGCGTCCATAAACCAATACAAGACCCCTCGCTGTGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTTCGTTGTGTCCAATTATTTCAGCCCAAAATGTCCCAATTGGGTTCCTGGTCGAAAAGAATGGTCTGCCAACAGTTAA

Coding sequence (CDS)

ATGCTGGAGGCTTCAAACAACTTACCCTCCAAACTTCCTAGCTTTGACATTCATCATTCCCATGAGAAAGATGAACTGGCAATATTGCAGCACATTGAAGAAGTTACAACTCATGCCGATCAAGTTCAACGACGAGTTCTTACAGAAATTCTCTGGCGCAACGCCCATGTCGAGTACCTGCAACAACATGGCCTCAATGGTTGTACCGACAGGAAGACCTTCAAGAAGATGATGCCCGTCGTCTCCTACGAGAATTTAAAATCATACGTGGATCGAATTGCAAATGGCGATACTTCTCCCATACTGTGTTCTGAACCCATATCAGAATTCTTAACCAGTTCGGGAACATCAGGAGGAGAGAGGAAGTTAATGCCAACAACCGAAGAAGAGCTCAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAGTTCATTCCAGGCTTGGACAAGGGCAAAGGAATGTACTTTTTGTTCGTAAAATCTGAATCCACAACTCCTGGAGGGTTACCTTCCCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACAACGATTACACAAGCCCTAATGAAACAATTCTTTGTACAGACTCATACCAAAGCATGTACTCTCAGTTCCTCTGTGGACTGTGTCTCAGCAGGCAAGTTCTTCGAGTTGGTGCTGTTTTTGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAAGTCATTGGGCCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCACTGACCCATTAGTGAGACAATCTGTCATGAACATTCTGAAACCTGACCCTGTGCTTGCTGACTTGATTGAATTTGAGTGCAGCAAAGAAACTTGGGCAGGTATTATAACCAGGCTGTGGCCAAATACCAAGTATGTTGATGTTATTGTGACAGGTACTATGTCACAGTACATCCCAACTTTAGATTACTATAGTAATGGACTCCCTCTTGTTTGTACTATGTATGCTTCTTCTGAGTGCTACTTTGGCATTAACTTAAATCCCTTGTGTAAGCCAAACCAGGTCTCTTACACCCTCATTCCCAACATGGCATATTTTGAATTTTTACCTCTTGATAGGACAAGTGGATTTGGCAGTACTTGTGAATCAGAAGCCATTGAGGAAATAAAGGATGAATTGGTTGATTTGGTGGATGTTGAATTGGGAAAGGAATACGAGCTCGTTATCACCACTTATGCAGGGCTTTATCGGTATCGAGTCGGCGACATTCTTCGAGTATCAGGATTCAAGAACAACGCCCCACAATTCAACTTCGTATGCAGGAAAAACGTCATTCTGAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAGAACGCAGTAATGGAAGCTTCAAAAAACTTAATCCCATACGACACTTGCCTCCTCGAGTACACGAGCTACGCAGATACTCATACAGTTCCAGGCCACTACGTCATATATTGGGAAATTAGCCTAAAGGAAACCACCACCATTCCTCGTTTAGTGTTTGAAAAATGTTGTCTTGAAATCGAAGAATCACTCAACAGCGTGTACCGTCAAGGAAGAGTCTCAGACAAATCAATCGGCCCTTTGGAGATAAAGATTGTTGAGAATGGAACTTTCGACAAACTCATGGACTATGCCATCAATCATGGTGCGTCCATAAACCAATACAAGACCCCTCGCTGTGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTTCGTTGTGTCCAATTATTTCAGCCCAAAATGTCCCAATTGGGTTCCTGGTCGAAAAGAATGGTCTGCCAACAGTTAA

Protein sequence

MLEASNNLPSKLPSFDIHHSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSANS
Homology
[Close]
BLAST of Spg000467 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 523/592 (88.34%), Postives = 553/592 (93.41%), Query Frame = 0 

Query: 22  EKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVV 81
           E+DE AILQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVV
Sbjct: 10  ERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVV 69

Query: 82  SYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 141
           SYENLK Y+DRIANGD SPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL
Sbjct: 70  SYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 129

Query: 142 MPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTS 201
           MPVMNQFIPGL++GKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTS
Sbjct: 130 MPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTS 189

Query: 202 PNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTG 261
           PN+TILCTDSYQSMYSQFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTG
Sbjct: 190 PNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTG 249

Query: 262 TINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTM 321
           TINPRI+DPLVRQSVMNILKPDPVLADLIEFECSK TWAGII RLWPNTKY+DVIVTGTM
Sbjct: 250 TINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTM 309

Query: 322 SQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRT 381
           SQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR 
Sbjct: 310 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR- 369

Query: 382 SGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 441
                        E+ +ELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN AP
Sbjct: 370 -------------EMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAP 429

Query: 442 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVI 501
           QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNL+PY+T LLEYTSYADTHTVPGHYVI
Sbjct: 430 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYVI 489

Query: 502 YWEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMD 561
           YWEIS KET  IP LVFE CCL IEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKLMD
Sbjct: 490 YWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMD 549

Query: 562 YAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSA 614
           YAINHG+SINQYKTPRCVKHEPILELLNS+VVSNYFSP+CP WVPG+KEWS+
Sbjct: 550 YAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSS 587

[Close]
BLAST of Spg000467 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 523/590 (88.64%), Postives = 549/590 (93.05%), Query Frame = 0 

Query: 23  KDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVS 82
           KDE A+LQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YENLK Y+DRIANGD SPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSP 202
           PVMNQFIPGL+KGKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDPVLADLIEFECSK TWAGII RLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTS 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 442
                       ++ +ELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN APQ
Sbjct: 364 ------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQ 423

Query: 443 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIY 502
           FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY+T LLEYTSYADTHTVPGHYVIY
Sbjct: 424 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIY 483

Query: 503 WEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDY 562
           WEIS KET  IP LVFE CCL IEESLNSVYRQGRVSDKSIGPLEIKI+ENGTFDKLMDY
Sbjct: 484 WEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDY 543

Query: 563 AINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           AINHG+SINQYKTPRCVKHEPILELLNS+VVSNYFSP+CP WVPG+KEWS
Sbjct: 544 AINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWS 579

[Close]
BLAST of Spg000467 vs. NCBI nr
Match: KAG6604833.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 522/590 (88.47%), Postives = 550/590 (93.22%), Query Frame = 0 

Query: 23  KDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVS 82
           KDE A+LQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YENLK Y+DRIANGD SPILCSEP+SEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSP 202
           PVMNQFIPGL+KGKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFECSK TWAGII RLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTS 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 442
                       ++ +ELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN APQ
Sbjct: 364 ------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQ 423

Query: 443 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIY 502
           FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY+T LLEYTSYADTHTVPGHYVIY
Sbjct: 424 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIY 483

Query: 503 WEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDY 562
           WEIS KET  IP LVFE CCL IEESLNSVYRQGRVSDKSIGPLEIKI+ENGTFDKLMDY
Sbjct: 484 WEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDY 543

Query: 563 AINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           AINHG+SINQYKTPRCVKHEPILELLNS+VVSNYFSP+CP WVPG+KEWS
Sbjct: 544 AINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGKKEWS 579

[Close]
BLAST of Spg000467 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 522/590 (88.47%), Postives = 549/590 (93.05%), Query Frame = 0 

Query: 23  KDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVS 82
           KDE A+LQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YENLK Y+DRIANGD SPILCSEPISEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSP 202
           PVMNQFIPGL+KGKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFECSK TWAGIITRLWPNTKYVDVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTS 382
           QYIPTLD YSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 442
                       ++ +ELVDLVDVELGKEYEL+ITTY+GLYRYRVGDILRVSGFKN APQ
Sbjct: 364 ------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQ 423

Query: 443 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIY 502
           FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY+T LLEYTSYADTHTVPGHYVIY
Sbjct: 424 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIY 483

Query: 503 WEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDY 562
           WEIS KET  IP  VFE CCL IEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKLMDY
Sbjct: 484 WEISQKETNAIPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDY 543

Query: 563 AINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           AINHG+SINQYKTPRCVK EPILELLNS+VVSNYFSPKCP WVPG+KEWS
Sbjct: 544 AINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWS 579

[Close]
BLAST of Spg000467 vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.3e-307
Identity = 529/622 (85.05%), Postives = 564/622 (90.68%), Query Frame = 0 

Query: 1   MLEASNNLPSKLPSFDIHHSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYL 60
           M EASNNLP + P   I  SHEK E A LQ+IE+VT HADQVQRRVLTEIL  NAHVEYL
Sbjct: 1   MPEASNNLPPE-PDSLIGSSHEKHERA-LQYIEQVTAHADQVQRRVLTEILSANAHVEYL 60

Query: 61  QQHGLNGCTDRKTFKKMMPVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGE 120
            +HGL+G  D KTFKK MPVVSY+++K YVDRIANGD SPILCS+PISEFLTSSGTSGGE
Sbjct: 61  -RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLT 180
           RKLMPTTEEEL+R+S LYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGL +RPVLT
Sbjct: 121 RKLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLT 180

Query: 181 SYYKSSHFKDRPYDLYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGF 240
           SYY+S HFKDRPYD YN YTSPNETILCTDSYQSMYSQFLCGLCLSR+VLRVGAVFASGF
Sbjct: 181 SYYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGF 240

Query: 241 IRAIKFLESHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWA 300
           IRAIKFLE+HW LL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIE ECSK TW 
Sbjct: 241 IRAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWE 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPN 360
           GII+RLW NTKY+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLC P+
Sbjct: 301 GIISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPS 360

Query: 361 QVSYTLIPNMAYFEFLPLDRTSGFG--STCESEAIEEIKDELVDLVDVELGKEYELVITT 420
           +VSYTLIP MAYFEFLPLD T+GFG  STC+SEA ++   ELVDLVDV+LGKEYELVITT
Sbjct: 361 EVSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITT 420

Query: 421 YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP 480
           YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP
Sbjct: 421 YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP 480

Query: 481 YDTCLLEYTSYADTHTVPGHYVIYWEISLKETT-----TIPRLVFEKCCLEIEESLNSVY 540
           Y  C++EYTSYADT T+PGHYVIYWEISLKE T      IP  VFEKCCL +EESLNSVY
Sbjct: 481 YGACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNSVY 540

Query: 541 RQGRVSDKSIGPLEIKIVENGTFDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVV 600
           RQGRVSDKSIGPLE+KIVE GTFDKLMDYA+NHGASINQYKTPRCVKH PILELLNS+VV
Sbjct: 541 RQGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVV 600

Query: 601 SNYFSPKCPNWVPGRKEWSANS 616
            NYFSPKCP+WVPGRKEWS N+
Sbjct: 601 DNYFSPKCPHWVPGRKEWSTNN 619

[Close]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 5.2e-270
Identity = 448/588 (76.19%), Postives = 507/588 (86.22%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKS 88
           LQ IE+VTT+AD VQRRVL EIL RNA VEYL++HGL G TDR+TFK +MPVV+YE+++ 
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 89  YVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            ++RIANGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 149 IPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILC 208
           +PGLDKGKGMYFLF+KSES TPGGLP+RPVLTSYYKSSHFK+RPYD Y +YTSPN+TILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 209 TDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  LA DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 328
           D  VR++V  ILKPDP LAD +E EC K +W GIITRLWPNTKYVDVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTC 388
           DYYSNGLPLVCTMYASSECYFG+NL PLCKP++VSYTLIPNMAYFEFLP+ R SG  S+ 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 389 E--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 448
                  E+ + ELVDLVDV+LG+EYELV+TTYAGLYRYRVGD+L V+GFKNNAPQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 449 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEIS 508
           CRKNV+LSIDSDK DEVELQNAV  A  +L+P+D  L EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINH 568
           L   T IP  VFE CCL IEESLNSVYRQGRVSDKSIGPLEIK+VE+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 569 GASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSAN 615
           GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP W PG K+W +N
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGSN 612

[Close]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 7.1e-267
Identity = 441/588 (75.00%), Postives = 506/588 (86.05%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKS 88
           LQ IEE+T++ADQVQR+VL EIL RNA VEYL++H LNG TDR+TFK +MPV++YE+++ 
Sbjct: 25  LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 89  YVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            ++RIANGD SPIL S+PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 149 IPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILC 208
           +PGL+ GKGMYFLF+KSES TPGGLP+RPVLTSYYKSSHFK+RPYD Y +YTSPNETILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 209 TDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  L  DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 328
           DP VR++V  ILKP P LAD +EFEC K +W GIITRLWPNTKYVDVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTC 388
           DYYSNGLPLVCTMYASSECYFG+NL PLCKP++VSYTLIP+MAYFEFLP+ R +G  ++ 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 389 E--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 448
                  E+ + ELVDLVDV+LG+EYELV+TTYAGL RYRVGD+LRV+GFKN APQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 449 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEIS 508
           CRKNV+LSIDSDK DEVELQNAV  A  +L+P+D  L EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINH 568
           L   T IP  VFE CCL +EES N+VYRQGRVSDKSIGPLEIKIVE GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 569 GASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSAN 615
           GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP WVPG K+W +N
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQWGSN 612

[Close]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 3.0e-225
Identity = 377/585 (64.44%), Postives = 457/585 (78.12%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTD-RKTFKKMMPVVSYENLK 88
           L+ IE VT +A QVQRRVL EIL +NA  EYL+++G+ G  D    F++++P+V+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 89  SYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 148
             + RIANGDTSPI   +PISEFLTSSGTSGGERKLMPT  +E+ RRS LYSLLMPVM+Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 149 FIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETIL 208
            + GLDKGK MY LFVK+ES TPGGL +RPVLTSYY+S  F DRP D Y  YTSP+E IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 209 CTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRI 268
           C DSYQSMY+Q LCGL     VLRVGAVFASGF+RAI FLE HWA L +DIRTG ++P I
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEI 263

Query: 269 TDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPT 328
           TD +VR +V  +L+ DP LAD IE EC++ +W GII RLWP TKY+DVIVTGTMSQYIPT
Sbjct: 264 TDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPT 323

Query: 329 LDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGST 388
           L++Y  GLPL CTMYASSECYFG+NLNP+CKP+ V+YTLIP M Y+EFLP++        
Sbjct: 324 LEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVN-------- 383

Query: 389 CESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFVC 448
           C +   E    +LVDLVDV+LG EYELV+TTY+GLYRYRVGD+LRV+GFKN AP F+FV 
Sbjct: 384 CNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVR 443

Query: 449 RKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEISL 508
           R+NV LS+DSDK DE EL  AV  A ++L P+   L+EYTSYAD  T+PGHYV++WE+  
Sbjct: 444 RQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELR- 503

Query: 509 KETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINHG 568
             +T +P  VFE+CCL +EE+LNSVYRQGR  D+SIGPLEI++V  GTFDKLMDYAI+ G
Sbjct: 504 AGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRG 563

Query: 569 ASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           ASINQYK PRCV+  P++ELL++ V   YFSPKCP W PG K+W+
Sbjct: 564 ASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWN 599

[Close]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 4.1e-222
Identity = 382/592 (64.53%), Postives = 458/592 (77.36%), Query Frame = 0 

Query: 22  EKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVV 81
           EKD  A L+ IEE+T +AD VQ  +L EIL RNA  EYL++  L G TDR TFK  +PV+
Sbjct: 17  EKDAKA-LRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVI 76

Query: 82  SYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 141
           +YE+L+  + RIA+GD SPIL + PISEFLTSSGTS GERKLMPT +EEL RR  LYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 142 MPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTS 201
           MPVMN ++PGLDKGKGMYFLFVKSE+ TPGGLP+RPVLTSYYKS HF+ RPYD YN YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 202 PNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTG 261
           PNE ILC DS+QSMY+Q LCGL     VLRVGAVFASG +RAI+FL+ HW+  A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 262 TINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTM 321
            ++  ITDP +RQ +  ILKPDPVLA+ I  EC  + W  IITR+WPNTKY+DVIVTG M
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 322 SQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRT 381
           +QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KP++VSYT++PNMAYFEF+PL  T
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 382 SGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 441
                            + V+LVDV +GKEYELV+TTYAGL RYRVGDILRV+GF N+AP
Sbjct: 377 -----------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAP 436

Query: 442 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTC---LLEYTSYADTHTVPGH 501
           QF+FV RKNV+LSIDSDK DE ELQ AV  AS   I ++ C   + EYTSYADT T+PGH
Sbjct: 437 QFHFVRRKNVLLSIDSDKTDESELQKAVENASS--ILHEECGSRVAEYTSYADTSTIPGH 496

Query: 502 YVIYWEISLKETTTIP-RLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFD 561
           YV+YWE+ +++    P      +CCL +EESLNSVYRQ RV+D S+GPLEI++V NGTF+
Sbjct: 497 YVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFE 556

Query: 562 KLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRK 610
           +LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 557 ELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

[Close]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 770.0 bits (1987), Expect = 2.0e-221
Identity = 374/583 (64.15%), Postives = 454/583 (77.87%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKS 88
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GL+G TDR  F+  +PVVSY++L+ 
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 89  YVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
           Y+ RIANGD SPIL + P+SEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 149 IPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILC 208
           +PGLDKGKG+YFLFVKSE+ TPGGL +RPVLTSYYKS HFK+RPYD Y++YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 209 TDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRIT 268
            D++QSMY+Q +CGLC    VLR+GAVFASG +RAI+FL+ +W  LA+DI +G + PR+T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 328
           DP VR++V  IL PDP LA LI  ECSK  WAGIITR+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 329 DYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTC 388
           ++YS GLP+ CTMYASSECYFG+NL P+C P++VSYT++PNM YFEFLP+D T       
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG------ 389

Query: 389 ESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFVCR 448
              A      +LVDL  VE+G+EYELVITTYAGL RYRVGD+LRV+GF N APQF FV R
Sbjct: 390 ---AASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRR 449

Query: 449 KNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEISLK 508
           KNV+LSI+SDK DE ELQ AV  AS  L P+   ++EYTS A T  +PGHYVIYWE+  K
Sbjct: 450 KNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTK 509

Query: 509 --ETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINH 568
               T +      +CCLE+EE+LN+VYRQ RV+D SIGPLEI++V  GTF++LMDYAI+ 
Sbjct: 510 GAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISR 569

Query: 569 GASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRK 610
           GASINQYK PRCV   PI+ELL+S VVS++FSP  P+W P R+
Sbjct: 570 GASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 523/590 (88.64%), Postives = 549/590 (93.05%), Query Frame = 0 

Query: 23  KDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVS 82
           KDE A+LQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YENLK Y+DRIANGD SPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSP 202
           PVMNQFIPGL+KGKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDPVLADLIEFECSK TWAGII RLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTS 382
           QYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 442
                       ++ +ELVDLVDVELGKEYELVITTY+GLYRYRVGDILRVSGFKN APQ
Sbjct: 364 ------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQ 423

Query: 443 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIY 502
           FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY+T LLEYTSYADTHTVPGHYVIY
Sbjct: 424 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIY 483

Query: 503 WEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDY 562
           WEIS KET  IP LVFE CCL IEESLNSVYRQGRVSDKSIGPLEIKI+ENGTFDKLMDY
Sbjct: 484 WEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDY 543

Query: 563 AINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           AINHG+SINQYKTPRCVKHEPILELLNS+VVSNYFSP+CP WVPG+KEWS
Sbjct: 544 AINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWS 579

[Close]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 522/590 (88.47%), Postives = 549/590 (93.05%), Query Frame = 0 

Query: 23  KDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVS 82
           KDE A+LQHIEE+TTHADQVQRRVLTEIL RNAHVEYLQQ GL+GC DR  FK ++PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YENLK Y+DRIANGD SPILCSEPISEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSP 202
           PVMNQFIPGL+KGKGMYFLFVKSESTTPGGLP+RPVLTSYYKSSHFKDRPYDLY DYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLS+QVLRVG+VFASGFIRAIKFLE+HW LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFECSK TWAGIITRLWPNTKYVDVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTS 382
           QYIPTLD YSNGLPLVCTMYASSECYFG+NLNPLC PN+VSYTLIP MAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQ 442
                       ++ +ELVDLVDVELGKEYEL+ITTY+GLYRYRVGDILRVSGFKN APQ
Sbjct: 364 ------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQ 423

Query: 443 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIY 502
           FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPY+T LLEYTSYADTHTVPGHYVIY
Sbjct: 424 FNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIY 483

Query: 503 WEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDY 562
           WEIS KET  IP  VFE CCL IEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKLMDY
Sbjct: 484 WEISQKETNAIPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDY 543

Query: 563 AINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWS 613
           AINHG+SINQYKTPRCVK EPILELLNS+VVSNYFSPKCP WVPG+KEWS
Sbjct: 544 AINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWS 579

[Close]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.1e-307
Identity = 529/622 (85.05%), Postives = 564/622 (90.68%), Query Frame = 0 

Query: 1   MLEASNNLPSKLPSFDIHHSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYL 60
           M EASNNLP + P   I  SHEK E A LQ+IE+VT HADQVQRRVLTEIL  NAHVEYL
Sbjct: 1   MPEASNNLPPE-PDSLIGSSHEKHERA-LQYIEQVTAHADQVQRRVLTEILSANAHVEYL 60

Query: 61  QQHGLNGCTDRKTFKKMMPVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGE 120
            +HGL+G  D KTFKK MPVVSY+++K YVDRIANGD SPILCS+PISEFLTSSGTSGGE
Sbjct: 61  -RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLT 180
           RKLMPTTEEEL+R+S LYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGL +RPVLT
Sbjct: 121 RKLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLT 180

Query: 181 SYYKSSHFKDRPYDLYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGF 240
           SYY+S HFKDRPYD YN YTSPNETILCTDSYQSMYSQFLCGLCLSR+VLRVGAVFASGF
Sbjct: 181 SYYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGF 240

Query: 241 IRAIKFLESHWALLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWA 300
           IRAIKFLE+HW LL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIE ECSK TW 
Sbjct: 241 IRAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGTWE 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPN 360
           GII+RLW NTKY+DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLC P+
Sbjct: 301 GIISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPS 360

Query: 361 QVSYTLIPNMAYFEFLPLDRTSGFG--STCESEAIEEIKDELVDLVDVELGKEYELVITT 420
           +VSYTLIP MAYFEFLPLD T+GFG  STC+SEA ++   ELVDLVDV+LGKEYELVITT
Sbjct: 361 EVSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITT 420

Query: 421 YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP 480
           YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP
Sbjct: 421 YAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIP 480

Query: 481 YDTCLLEYTSYADTHTVPGHYVIYWEISLKETT-----TIPRLVFEKCCLEIEESLNSVY 540
           Y  C++EYTSYADT T+PGHYVIYWEISLKE T      IP  VFEKCCL +EESLNSVY
Sbjct: 481 YGACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNSVY 540

Query: 541 RQGRVSDKSIGPLEIKIVENGTFDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVV 600
           RQGRVSDKSIGPLE+KIVE GTFDKLMDYA+NHGASINQYKTPRCVKH PILELLNS+VV
Sbjct: 541 RQGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVV 600

Query: 601 SNYFSPKCPNWVPGRKEWSANS 616
            NYFSPKCP+WVPGRKEWS N+
Sbjct: 601 DNYFSPKCPHWVPGRKEWSTNN 619

[Close]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match: A0A1U7YQ53 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=3 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 5.6e-275
Identity = 459/597 (76.88%), Postives = 519/597 (86.93%), Query Frame = 0 

Query: 17  IHHSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKK 76
           I H+        L+ IE+VTT+AD++Q+RVL EIL R+AHVEYLQ+HGLNG TDR+TFKK
Sbjct: 19  IDHNRPDVNQNALEFIEDVTTNADEIQKRVLAEILSRSAHVEYLQRHGLNGRTDRETFKK 78

Query: 77  MMPVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSF 136
           +MPVV+YE+L+S ++RIANGDTSPILC  PISEFLTSSGTS GERKLMPT EEEL+RRS 
Sbjct: 79  VMPVVTYEDLQSDINRIANGDTSPILCGLPISEFLTSSGTSAGERKLMPTIEEELERRSL 138

Query: 137 LYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLY 196
           LYSLLMPVM+QF+PGLDKGKGMYFLF+KSE+ TPGGL +RPVLTSYYKS HFK RPYD Y
Sbjct: 139 LYSLLMPVMDQFVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSLHFKHRPYDPY 198

Query: 197 NDYTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLAN 256
            +YTSPNETILC DSYQSMYSQ LCGLC ++QVLRVGAVFASGFIRAI+FLE HW+LL N
Sbjct: 199 TNYTSPNETILCPDSYQSMYSQLLCGLCQNKQVLRVGAVFASGFIRAIRFLEKHWSLLCN 258

Query: 257 DIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVI 316
           DIRTG INP+ITDP VR +V+ ILKPDP LAD IE EC++E W GIITRLWPNTKY+DVI
Sbjct: 259 DIRTGAINPQITDPSVRDAVLRILKPDPELADFIESECTREAWQGIITRLWPNTKYIDVI 318

Query: 317 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFL 376
           VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLCKPN+VSYTLIP MAYFEFL
Sbjct: 319 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPNEVSYTLIPTMAYFEFL 378

Query: 377 PLDRTSGFGSTCE--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVS 436
           P+ R +GF ++        E+ + +LVDLVDV+LG+EYELV+TTYAGLYRYRVGD+LRV+
Sbjct: 379 PVHRNNGFTTSISVPKSLNEKEQQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVA 438

Query: 437 GFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHT 496
           GFKN APQFNFVCRKNV+LSIDSDK DEVELQ AV +A  +L+P+D  L+EYTSYADT T
Sbjct: 439 GFKNKAPQFNFVCRKNVVLSIDSDKTDEVELQAAVKDAINHLVPFDASLIEYTSYADTTT 498

Query: 497 VPGHYVIYWEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENG 556
           +PGHYV+YWE+    TT IP  VFE CCL IEESLNSVYRQGRVSDKSIGPLEIKIVE G
Sbjct: 499 IPGHYVLYWELRHDSTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVETG 558

Query: 557 TFDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEW 612
           TFDKLMDYAI+ GASINQYKTPRCVK  PI+ELLNS V+SNYFSPKCP WVPG K+W
Sbjct: 559 TFDKLMDYAISRGASINQYKTPRCVKFAPIVELLNSRVLSNYFSPKCPKWVPGHKQW 615

[Close]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match: A0A6A3AIU7 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110472pilonHSYRG00040 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 2.1e-274
Identity = 460/595 (77.31%), Postives = 515/595 (86.55%), Query Frame = 0 

Query: 19  HSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMM 78
           +S E      LQ IE+VT+  D VQR+VL EIL RNAHVEYLQ+HGLNG TDR +FKK+M
Sbjct: 14  YSLELKNKKTLQFIEDVTSSPDGVQRKVLEEILERNAHVEYLQKHGLNGHTDRGSFKKIM 73

Query: 79  PVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLY 138
           PV++YE+++ YV+RIANGDTSPILCS+PISEFLTSSGTSGGERKLMPT EEEL RRS LY
Sbjct: 74  PVITYEDIQPYVERIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLY 133

Query: 139 SLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYND 198
           SLLMPVM+QF+PGLDKGKGMYFLF+KSE+ TPGGL +RPVLTSYYKS HFKDRPYD Y +
Sbjct: 134 SLLMPVMSQFVPGLDKGKGMYFLFIKSEAKTPGGLAARPVLTSYYKSYHFKDRPYDPYTN 193

Query: 199 YTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDI 258
           YTSPNETILC DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW LL NDI
Sbjct: 194 YTSPNETILCPDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWRLLCNDI 253

Query: 259 RTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVT 318
           RTGTI  +ITD  VR++VMNILKPDP LAD IE ECSK++W GIITRLWPNTKYVDVIVT
Sbjct: 254 RTGTIGSQITDQSVREAVMNILKPDPELADFIEAECSKDSWQGIITRLWPNTKYVDVIVT 313

Query: 319 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPL 378
           GTMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLCKP++VSYTLIP MAYFEFLP+
Sbjct: 314 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPI 373

Query: 379 DRTSGFGSTCE--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGF 438
            R +G  ++        E+ + ELVDLVDV+LGKEYELV+TTY+GLYRYRVGD+LRV+GF
Sbjct: 374 QRNNGVSNSISVPKTLNEKEQQELVDLVDVKLGKEYELVVTTYSGLYRYRVGDVLRVAGF 433

Query: 439 KNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVP 498
           KNNAPQFNF+CRKNV+L IDSDK DEVELQN+V  A  +L+P+D  L EYTSYADT T+P
Sbjct: 434 KNNAPQFNFICRKNVVLCIDSDKTDEVELQNSVKNAVNHLLPFDATLAEYTSYADTTTIP 493

Query: 499 GHYVIYWEISLKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTF 558
           GHYV+YWE+SL  TT IP  VFE CCL IEESLNSVYRQGRV DKSIGPLEIKIVE GTF
Sbjct: 494 GHYVLYWELSLNGTTPIPPSVFEDCCLTIEESLNSVYRQGRVCDKSIGPLEIKIVEPGTF 553

Query: 559 DKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEW 612
           DKLMDYAI+ GASINQYKTPRCVK+ PI+ELLNS  VS+YFSPKCP W PG K+W
Sbjct: 554 DKLMDYAISLGASINQYKTPRCVKYAPIVELLNSMAVSSYFSPKCPRWFPGHKQW 608

[Close]
BLAST of Spg000467 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 931.4 bits (2406), Expect = 3.7e-271
Identity = 448/588 (76.19%), Postives = 507/588 (86.22%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKS 88
           LQ IE+VTT+AD VQRRVL EIL RNA VEYL++HGL G TDR+TFK +MPVV+YE+++ 
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 89  YVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            ++RIANGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 149 IPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILC 208
           +PGLDKGKGMYFLF+KSES TPGGLP+RPVLTSYYKSSHFK+RPYD Y +YTSPN+TILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 209 TDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  LA DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 328
           D  VR++V  ILKPDP LAD +E EC K +W GIITRLWPNTKYVDVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTC 388
           DYYSNGLPLVCTMYASSECYFG+NL PLCKP++VSYTLIPNMAYFEFLP+ R SG  S+ 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 389 E--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 448
                  E+ + ELVDLVDV+LG+EYELV+TTYAGLYRYRVGD+L V+GFKNNAPQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 449 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEIS 508
           CRKNV+LSIDSDK DEVELQNAV  A  +L+P+D  L EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINH 568
           L   T IP  VFE CCL IEESLNSVYRQGRVSDKSIGPLEIK+VE+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 569 GASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSAN 615
           GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP W PG K+W +N
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGSN 612

[Close]
BLAST of Spg000467 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 921.0 bits (2379), Expect = 5.0e-268
Identity = 441/588 (75.00%), Postives = 506/588 (86.05%), Query Frame = 0 

Query: 29  LQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVVSYENLKS 88
           LQ IEE+T++ADQVQR+VL EIL RNA VEYL++H LNG TDR+TFK +MPV++YE+++ 
Sbjct: 25  LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 89  YVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            ++RIANGD SPIL S+PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 149 IPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTSPNETILC 208
           +PGL+ GKGMYFLF+KSES TPGGLP+RPVLTSYYKSSHFK+RPYD Y +YTSPNETILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 209 TDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  L  DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTMSQYIPTL 328
           DP VR++V  ILKP P LAD +EFEC K +W GIITRLWPNTKYVDVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRTSGFGSTC 388
           DYYSNGLPLVCTMYASSECYFG+NL PLCKP++VSYTLIP+MAYFEFLP+ R +G  ++ 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 389 E--SEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 448
                  E+ + ELVDLVDV+LG+EYELV+TTYAGL RYRVGD+LRV+GFKN APQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 449 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVPGHYVIYWEIS 508
           CRKNV+LSIDSDK DEVELQNAV  A  +L+P+D  L EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETTTIPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFDKLMDYAINH 568
           L   T IP  VFE CCL +EES N+VYRQGRVSDKSIGPLEIKIVE GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 569 GASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRKEWSAN 615
           GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP WVPG K+W +N
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQWGSN 612

[Close]
BLAST of Spg000467 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 774.6 bits (1999), Expect = 5.8e-224
Identity = 382/603 (63.35%), Postives = 470/603 (77.94%), Query Frame = 0 

Query: 10  SKLPSFDIHHSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCT 69
           S L S  +  +  + ++  L+ IEE+T + D VQ +VL EIL RN++ EYL++  L+G  
Sbjct: 5   SPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVV 64

Query: 70  DRKTFKKMMPVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEE 129
           DRKTFK  +PVV+YE+LK  + RI+NGD SPIL S PI+EFLTSSGTS GERKLMPT EE
Sbjct: 65  DRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEE 124

Query: 130 ELKRRSFLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFK 189
           +L RR  LYSLLMPVMN ++PGLDKGKG+YFLFVKSES T GGLP+RPVLTSYYKS HFK
Sbjct: 125 DLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFK 184

Query: 190 DRPYDLYNDYTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLES 249
            RPYD YN YTSPNE ILC+DS QSMY+Q LCGL +  +VLR+GAVFASG +RAI FL++
Sbjct: 185 RRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQN 244

Query: 250 HWALLANDIRTGTINPRITDPLVRQSVMNIL-KPDPVLADLIEFECSKETWAGIITRLWP 309
           +W  LA DI TGT++ RI DP ++  +  IL KPD  LA+ +   CS+E W GIIT++WP
Sbjct: 245 NWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWP 304

Query: 310 NTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIP 369
           NTKY+DVIVTG M+QYIPTL+YYS GLP+ CTMYASSE YFGINL P+CKP++VSYT++P
Sbjct: 305 NTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMP 364

Query: 370 NMAYFEFLPLDRTSGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVG 429
           NMAYFEFLP +     G      +++E    LV+L +VE+GKEYELVITTYAGLYRYRVG
Sbjct: 365 NMAYFEFLPHNHD---GDGAAEASLDE--TSLVELANVEVGKEYELVITTYAGLYRYRVG 424

Query: 430 DILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTS 489
           DILRV+GF N+APQF F+ RKNV+LS++SDK DE ELQ AV  AS+      T ++EYTS
Sbjct: 425 DILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTS 484

Query: 490 YADTHTVPGHYVIYWEISLKETTT--IPRLVFEKCCLEIEESLNSVYRQGRVSDKSIGPL 549
           YA+T T+PGHYVIYWE+  ++ +   +   V  KCCLE+EESLNSVYRQ RV+DKSIGPL
Sbjct: 485 YAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPL 544

Query: 550 EIKIVENGTFDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVP 609
           EI++V NGTF++LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P
Sbjct: 545 EIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSP 602

[Close]
BLAST of Spg000467 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 772.3 bits (1993), Expect = 2.9e-223
Identity = 382/592 (64.53%), Postives = 458/592 (77.36%), Query Frame = 0 

Query: 22  EKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMMPVV 81
           EKD  A L+ IEE+T +AD VQ  +L EIL RNA  EYL++  L G TDR TFK  +PV+
Sbjct: 17  EKDAKA-LRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVI 76

Query: 82  SYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 141
           +YE+L+  + RIA+GD SPIL + PISEFLTSSGTS GERKLMPT +EEL RR  LYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 142 MPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYNDYTS 201
           MPVMN ++PGLDKGKGMYFLFVKSE+ TPGGLP+RPVLTSYYKS HF+ RPYD YN YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 202 PNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDIRTG 261
           PNE ILC DS+QSMY+Q LCGL     VLRVGAVFASG +RAI+FL+ HW+  A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 262 TINPRITDPLVRQSVMNILKPDPVLADLIEFECSKETWAGIITRLWPNTKYVDVIVTGTM 321
            ++  ITDP +RQ +  ILKPDPVLA+ I  EC  + W  IITR+WPNTKY+DVIVTG M
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 322 SQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFLPLDRT 381
           +QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KP++VSYT++PNMAYFEF+PL  T
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 382 SGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGFKNNAP 441
                            + V+LVDV +GKEYELV+TTYAGL RYRVGDILRV+GF N+AP
Sbjct: 377 -----------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAP 436

Query: 442 QFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTC---LLEYTSYADTHTVPGH 501
           QF+FV RKNV+LSIDSDK DE ELQ AV  AS   I ++ C   + EYTSYADT T+PGH
Sbjct: 437 QFHFVRRKNVLLSIDSDKTDESELQKAVENASS--ILHEECGSRVAEYTSYADTSTIPGH 496

Query: 502 YVIYWEISLKETTTIP-RLVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGTFD 561
           YV+YWE+ +++    P      +CCL +EESLNSVYRQ RV+D S+GPLEI++V NGTF+
Sbjct: 497 YVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFE 556

Query: 562 KLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRK 610
           +LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 557 ELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

[Close]
BLAST of Spg000467 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 763.8 bits (1971), Expect = 1.0e-220
Identity = 380/594 (63.97%), Postives = 464/594 (78.11%), Query Frame = 0 

Query: 19  HSHEKDELAILQHIEEVTTHADQVQRRVLTEILWRNAHVEYLQQHGLNGCTDRKTFKKMM 78
           HS    ++  L+ IEE+T + D VQ++V+ EIL RN+  EYL++ GL G TDRKTFK  +
Sbjct: 13  HSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKV 72

Query: 79  PVVSYENLKSYVDRIANGDTSPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLY 138
           PVV Y++LK  + RIANGD S IL S PI+EFLTSSGTS GERKLMPT +E++ RR  LY
Sbjct: 73  PVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLY 132

Query: 139 SLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLPSRPVLTSYYKSSHFKDRPYDLYND 198
           SLLMPVMN ++PGLDKGK +YFLFVK+ES TPGGLP+RPVLTSYYKS  FK RP D YN 
Sbjct: 133 SLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNV 192

Query: 199 YTSPNETILCTDSYQSMYSQFLCGLCLSRQVLRVGAVFASGFIRAIKFLESHWALLANDI 258
           YTSPNE ILC DS QSMY+Q LCGL +  +VLR+GAVFASG +RAI FL+++W  LA+DI
Sbjct: 193 YTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDI 252

Query: 259 RTGTINPRITDPLVRQSVMNIL-KPDPVLADLIEFECSKE-TWAGIITRLWPNTKYVDVI 318
            TGT++ RI+DP +++S+  IL KPD  LAD I   C ++ +W GIIT++WPNTKY+DVI
Sbjct: 253 STGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVI 312

Query: 319 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCKPNQVSYTLIPNMAYFEFL 378
           VTG M+QYIP L+YYS GLP+ CTMYASSE YFGINL P+CKP++VSYT++PNMAYFEFL
Sbjct: 313 VTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFL 372

Query: 379 PLDRTSGFGSTCESEAIEEIKDELVDLVDVELGKEYELVITTYAGLYRYRVGDILRVSGF 438
           P               +   K ELV+L DVE+GKEYELVITTYAGL RYRVGDIL+V+GF
Sbjct: 373 P------------HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGF 432

Query: 439 KNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYDTCLLEYTSYADTHTVP 498
            N+APQF FV RKNV+LSI+SDK DE ELQ+AV  AS  L    T ++EYTSYA+T T+P
Sbjct: 433 YNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIP 492

Query: 499 GHYVIYWEISLKETTTIPR-LVFEKCCLEIEESLNSVYRQGRVSDKSIGPLEIKIVENGT 558
           GHYVIYWE+ +K+ T  P   V  +CCLE+EESLNSVYRQ RV+DKSIGPLEI++V+NGT
Sbjct: 493 GHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGT 552

Query: 559 FDKLMDYAINHGASINQYKTPRCVKHEPILELLNSFVVSNYFSPKCPNWVPGRK 610
           F++LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 553 FEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

The following BLAST results are available for this feature:
BLAST of Spg000467 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023533206.10.0e+0088.34indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
XP_022948165.10.0e+0088.64indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
KAG6604833.10.0e+0088.47Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022970970.10.0e+0088.47indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_022140599.12.3e-30785.05indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia... [more]
BLAST of Spg000467 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LSQ45.2e-27076.19Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818297.1e-26775.00Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM53.0e-22564.44Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
O823334.1e-22264.53Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
A3BLS02.0e-22164.15Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
BLAST of Spg000467 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G8Z20.0e+0088.64indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1I4E60.0e+0088.47indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1CG471.1e-30785.05indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charant... [more]
A0A1U7YQ535.6e-27576.88indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC10... [more]
A0A6A3AIU72.1e-27477.31Indole-3-acetic acid-amido synthetase GH3.6 OS=Hibiscus syriacus OX=106335 GN=F3... [more]
BLAST of Spg000467 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G54510.13.7e-27176.19Auxin-responsive GH3 family protein [more]
AT4G27260.15.0e-26875.00Auxin-responsive GH3 family protein [more]
AT4G37390.15.8e-22463.35Auxin-responsive GH3 family protein [more]
AT2G14960.12.9e-22364.53Auxin-responsive GH3 family protein [more]
AT2G23170.11.0e-22063.97Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 32..581
e-value: 2.0E-201
score: 670.5
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 20..611
NoneNo IPR availablePANTHERPTHR31901:SF63INDOLE-3-ACETIC ACID-AMIDO SYNTHETASEcoord: 20..611

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000467.1Spg000467.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity