Spg000388 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000388
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMembrane metalloprotease ARASP, chloroplastic
Locationscaffold8: 41008503 .. 41011073 (-)
RNA-Seq ExpressionSpg000388
SyntenySpg000388
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCTCCACCAGGTAAGGTGGGAAAAGTTATCACAGTCTCACGAGTTGATTATATTTCCTTATAGTACCCATTTTATTTGTTTACTTATTTCTTTTCAAAAAAAAAAAAAAATTGTTTAGGCCCCGTTTGGTTCATGATCCGTTTTCGTTTTTATGTTTTCGAAAATAAAAAATACAAGGGAAACCATGAAAACGTGTTTGGTATACCTATTTTCAAAAACAATTTTCAAAAACAACTCAAAAAATGACATGTTTTCAAAAATGATAAAATGTTGTTTTCATGTTTTCCAATTTTGTTTTCAAAATAATGGAAACACGAGATATAACTTTAAAAACACATGATACATCTTTCTTTCCATCAAATAATATTGCATTATCTACAAACAACATTTTTTATTTTTTGATATCCAAATTTTCTTCGACTCTGAAGGGAAAAAAAATACAAAATTACTGTTGTGTAAATAGTGTGTTTGATTAATATTGATGTTTTGGTTGTTATTTTTACTTTTTACATAATTCTACCTATTTTGATATACTCCACTTTACAAATCAAAAGTTTCATATAATACAACTATATGATATATCTTTTTTTTCCTTATAATTTCAATTCCAACAAAATATATATATATATTTTCAAGAATCAATTTTAGCATCATTGATTTACAATCTCTCTAAAAATGAATAAAAGTCAAATAGAAAAATAATCATATTAAATTAACTTTTTCTAAAAAAAAAAATAATTTTACCAAGCATATTAATATTTTCATGCATTTTCAAATTCATAACCAAACACAGTTTTCAAATTTAAAGTTTTTAAATTGGAACCAAACATGTTTTTAGATATAATGGAAATAAGAAATTTTTTTTCGTTTTCTACTGAATTCTTTATTTTCGAAAACAATTTTCAAATCACGAACCAAACGGGTTACTTATTTAGTGGACCTTGCTTTTTATTTTTATTATTTTTCCCAATAAAAGCAAGATCACACGTTCGAACCGTGGGGCGACCGAGGCCTCAAATGTCATAAACCTCTCAATAACACACACACAAATGCGAGGGGAAAAATGAGAAACACTTCTCTCACTTCCCCTCCACAAATTTCAAAAAGGTGTTATCCGAAACCTATTTCTTCTCCAATTCTTCCCCCTTCCTCTATTTCTCGCTAAATCTCTGTCAATCCTGATAACCCTCCACGAACTCTCAAAATCATGCTATTAAATCTATCTTCCTCTTTGTTTTCTGTTATCAAATTAGCGAATTCGAAGTCCCACTTCTCAGATTTTTCACTCAAGTCCAGAACCCACTTCTCAAAATCCCTATCTTCTTCTTCTTCTTCTTCGTTATCTCATTCTTTCTCTTCGTCGGACCTCAGTTTCAAGAACAGACACCAAATTTTCTGGGAAAAGAGCAGAATCCCATGTCGGAGGAGTGGCGATTTCAGGCTGTGGGCTCTTGCCGGAATCGATATCGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCGGTGTTGACGGCCATCATTGTTGTTCACGAGAGTGGCCACTTCCTCGCCGCCTGTCTACAGGGCATCCACGTAAGTAAATTTGCAATCGGTTTTGGTCCAATTCTTGCGAAATTCAACAAAAACAATGTGGAATACTCCATTAGGGCTTTCCCTCTCGGTGGATTTGTGGGATTTCCTGATAATGATCCTGAGAGCGATATTCCAGTGGACGACGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTTATTGTGATTTCTGCTGGTGTTATTGCTAATATAATCTTCGCTTATATAATTATCTTGATTCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCGTTCCCTGGTGTTCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTCCCCGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGAGCTCGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAATTGAAAGAGGGAAAGAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTCTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAAGCTTTTAGCTATTCTAAAAAGGAATTCTTGGGTCTGTCATATAATGTTTTGGATAGCTTGAAACAGACTTTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCTAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGACGGCGGTTCGCTGGCCTTAATCCTCATTGAGGCTGTAAGGGGAGGGAGAAAGCTGCCATTAGAACTCGAGCAGCGAATCATGTCATCTGGGATCATGTTCGTTGTACTTCTCGGGCTATTCCTCATCATCAAAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

mRNA sequence

ATGTTGCTCCACCAGGTAAGGGCTTTCCCTCTCGGTGGATTTGTGGGATTTCCTGATAATGATCCTGAGAGCGATATTCCAGTGGACGACGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTTATTGTGATTTCTGCTGGTGTTATTGCTAATATAATCTTCGCTTATATAATTATCTTGATTCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCGTTCCCTGGTGTTCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTCCCCGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGAGCTCGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAATTGAAAGAGGGAAAGAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTCTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAAGCTTTTAGCTATTCTAAAAAGGAATTCTTGGGTCTGTCATATAATGTTTTGGATAGCTTGAAACAGACTTTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCTAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGACGGCGGTTCGCTGGCCTTAATCCTCATTGAGGCTGTAAGGGGAGGGAGAAAGCTGCCATTAGAACTCGAGCAGCGAATCATGTCATCTGGGATCATGTTCGTTGTACTTCTCGGGCTATTCCTCATCATCAAAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Coding sequence (CDS)

ATGTTGCTCCACCAGGTAAGGGCTTTCCCTCTCGGTGGATTTGTGGGATTTCCTGATAATGATCCTGAGAGCGATATTCCAGTGGACGACGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTTATTGTGATTTCTGCTGGTGTTATTGCTAATATAATCTTCGCTTATATAATTATCTTGATTCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCGTTCCCTGGTGTTCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTCCCCGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGAGCTCGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAATTGAAAGAGGGAAAGAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTCTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAAGCTTTTAGCTATTCTAAAAAGGAATTCTTGGGTCTGTCATATAATGTTTTGGATAGCTTGAAACAGACTTTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCTAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGACGGCGGTTCGCTGGCCTTAATCCTCATTGAGGCTGTAAGGGGAGGGAGAAAGCTGCCATTAGAACTCGAGCAGCGAATCATGTCATCTGGGATCATGTTCGTTGTACTTCTCGGGCTATTCCTCATCATCAAAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Protein sequence

MLLHQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSYSKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDTLNLEFIKDLL
Homology
BLAST of Spg000388 vs. NCBI nr
Match: XP_038902635.1 (probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 559.7 bits (1441), Expect = 1.6e-155
Identity = 296/310 (95.48%), Postives = 308/310 (99.35%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 146 YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 205

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVS+LVEA
Sbjct: 206 QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEA 265

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERGK+DLEIGVTPDES DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 266 IKRSPNRTVLLKVERGKQDLEIGVTPDESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 325

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSY+VLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 326 SRKEFLGLSYSVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 385

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 386 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 445

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 446 LNLEFIKDLL 455

BLAST of Spg000388 vs. NCBI nr
Match: XP_004151933.2 (probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus])

HSP 1 Score: 554.3 bits (1427), Expect = 6.8e-154
Identity = 293/310 (94.52%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 147 YSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 206

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELPKLGSTAVS+LVEA
Sbjct: 207 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEA 266

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 267 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 326

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 327 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 386

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 387 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 446

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 447 LNLEFIKDLL 456

BLAST of Spg000388 vs. NCBI nr
Match: XP_008455887.1 (PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo] >TYK28920.1 putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa])

HSP 1 Score: 553.5 bits (1425), Expect = 1.2e-153
Identity = 293/310 (94.52%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 148 YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 207

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELP LGSTAVS+LVEA
Sbjct: 208 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEA 267

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 268 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 327

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 328 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 387

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 388 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 447

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 448 LNLEFIKDLL 457

BLAST of Spg000388 vs. NCBI nr
Match: KAA0034835.1 (putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa])

HSP 1 Score: 553.5 bits (1425), Expect = 1.2e-153
Identity = 293/310 (94.52%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 59  YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 118

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELP LGSTAVS+LVEA
Sbjct: 119 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEA 178

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 179 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 238

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 239 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 298

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 299 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 358

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 359 LNLEFIKDLL 368

BLAST of Spg000388 vs. NCBI nr
Match: KAG6570888.1 (Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551.6 bits (1420), Expect = 4.4e-153
Identity = 292/310 (94.19%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIP+DDENLLKNRPILDRV+VISAGVIANIIFAYIIILI
Sbjct: 140 YSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILI 199

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA
Sbjct: 200 QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 259

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG++DLEIGVTPDES DGTGRIGVQLSPNVKISK+VAKNFLEAFSY
Sbjct: 260 IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSY 319

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQT LNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 320 SRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 379

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEA RGGRKLPLELEQRIMSSGIM VVLLG+FLI++DT
Sbjct: 380 NLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDT 439

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 440 LNLEFIKDLL 449

BLAST of Spg000388 vs. ExPASy Swiss-Prot
Match: O23053 (Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP2 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-129
Identity = 239/310 (77.10%), Postives = 276/310 (89.03%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIPVDD NLLKNRPILDRVIV+SAG++AN+IFAY II  
Sbjct: 132 YSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRPILDRVIVVSAGIVANVIFAYAIIFT 191

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPGDVILAV+G EL   GS +VS++V+ 
Sbjct: 192 QVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPGDVILAVDGTELSNSGSDSVSKVVDV 251

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           +KR+P   VLL+IERGKE  EI +TPD+S DGTG+IGVQLSPNV+  KV  KN  E FS+
Sbjct: 252 VKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGKIGVQLSPNVRFGKVRPKNIPETFSF 311

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           + +EF GLSYNVLDSLKQTFLNFSQ+ASKV+GPVAIIAVGAEVARSN DGLYQFAA+LN+
Sbjct: 312 AGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVAIIAVGAEVARSNADGLYQFAALLNL 371

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGG+LALIL+EAVRGGRKLPLE+EQ IMSSGIM V+ LGLFLI+KDT
Sbjct: 372 NLAVINLLPLPALDGGTLALILLEAVRGGRKLPLEVEQGIMSSGIMLVLFLGLFLIVKDT 431

Query: 304 LNLEFIKDLL 314
           LNL+FIK++L
Sbjct: 432 LNLDFIKEML 441

BLAST of Spg000388 vs. ExPASy Swiss-Prot
Match: O80885 (Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 5.2e-125
Identity = 231/310 (74.52%), Postives = 273/310 (88.06%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+S+IP+DDENLLKNRP LDR IV+SAG+IAN+IFAY II +
Sbjct: 138 YSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFV 197

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVILAV+G EL K G  AVS++V+ 
Sbjct: 198 QVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDI 257

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           +KR+P   V+ +IERG ED +I VTPD++ DGTG+IGVQLSPNV+I+KV  +N  E F +
Sbjct: 258 VKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRF 317

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
             +EF+GLS NVLD LKQTF NFSQ+ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNI
Sbjct: 318 VGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNI 377

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGG+LALIL+EAVRGG+KLP+E+EQ IMSSGIM V+ LGLFLI+KDT
Sbjct: 378 NLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDT 437

Query: 304 LNLEFIKDLL 314
           L+L+FIK++L
Sbjct: 438 LSLDFIKEML 447

BLAST of Spg000388 vs. ExPASy Swiss-Prot
Match: P73714 (Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1821 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.0e-72
Identity = 144/310 (46.45%), Postives = 218/310 (70.32%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RA PLGG+V FPD+DP+S+IP DD NLLKNRPILDR IVISAGVIAN++FAY +++ 
Sbjct: 56  YSIRAIPLGGYVAFPDDDPDSEIPADDPNLLKNRPILDRAIVISAGVIANLVFAYFLLIG 115

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVS++G   Q   PG+++P+V + SAA   G+ PGD++L++ GN LP     A ++ ++ 
Sbjct: 116 QVSTIGF--QNIQPGLVIPQVDSASAAQVAGMEPGDIVLSLQGNTLPGF-PDATTQFIDI 175

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           ++RSP+  + ++++RG+E   + +TP    +G G+IGV L PNV+  +  A N LEA +Y
Sbjct: 176 VRRSPSVPITVEVQRGEETKTLTITPTPDAEGKGKIGVALLPNVETKR--ASNPLEALTY 235

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S + F  +         Q   NF+ +AS+V+GPV I+  GA +ARS+   L+QF A+++I
Sbjct: 236 SAEAFERIVKLTTQGFWQLISNFADNASQVAGPVKIVEYGANIARSDASNLFQFGALISI 295

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVIN+LPLPALDGG L  +LIE +  G+ LP + +  +M +G++ ++ LG+FLI++DT
Sbjct: 296 NLAVINILPLPALDGGQLVFLLIEGLL-GKPLPEKFQMGVMQTGLVLLLSLGVFLIVRDT 355

Query: 304 LNLEFIKDLL 314
           LNL F+++ L
Sbjct: 356 LNLTFVQEFL 359

BLAST of Spg000388 vs. ExPASy Swiss-Prot
Match: Q8YQ64 (Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=all3971 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.7e-64
Identity = 144/307 (46.91%), Postives = 204/307 (66.45%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPD+DP+SDIP +D NLL+NRPILDR IVISAGVIAN+IFAY+++L 
Sbjct: 53  YAIRAFPLGGFVGFPDDDPDSDIPPNDPNLLRNRPILDRAIVISAGVIANLIFAYMLLLA 112

Query: 64  QVSSVGLPVQEPFPGV----LVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSE 123
           QV  VG+  Q   PGV    L PEV   + A+  GL PGDVIL+ N  E        +  
Sbjct: 113 QVGFVGIG-QASQPGVSIQQLAPEVS--AVATNAGLKPGDVILSANQKEF-GTSLQEIEA 172

Query: 124 LVEAIKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLE 183
           L + IK SP +++ L + RG E L + V P+    G G IG+ L+PN K+ +    +  +
Sbjct: 173 LRDIIKNSPGKSIQLTVARGDERLSVNVIPEAKPAG-GSIGIGLAPNGKVERRPV-SLSK 232

Query: 184 AFSYSKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAA 243
           AFS    EF  +         Q   NF ++AS+V+GP+ I+ +GA +A+++   L+ FAA
Sbjct: 233 AFSVGASEFQRIVVMTFKGFGQLVTNFGETASQVAGPIKIVEIGANIAQNDTGSLFFFAA 292

Query: 244 VLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLI 303
           +++INLAVIN+LPLPALDGG LA +LIE +R G+ LP  +++ +M +G++ ++ LG+FLI
Sbjct: 293 LISINLAVINILPLPALDGGQLAFLLIEGLR-GKPLPNRIQEGVMQTGLVLLLGLGIFLI 352

Query: 304 IKDTLNL 307
           +K+T  L
Sbjct: 353 VKETTQL 352

BLAST of Spg000388 vs. ExPASy Swiss-Prot
Match: O31754 (Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 GN=rasP PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.5e-26
Identity = 91/275 (33.09%), Postives = 140/275 (50.91%), Query Frame = 0

Query: 36  NRPILDRVIVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGL 95
           ++P+  R+  I+AG I N I AY+I+++     G+P  EP  G L    R    A+  GL
Sbjct: 165 SKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGR----AAEAGL 224

Query: 96  LPGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKIERGKEDLEIGVTP----DE 155
             GD I ++NG ++      + +++V A+K +P + + + ++R  + L I VTP    DE
Sbjct: 225 KEGDYIQSINGEKM-----RSWTDIVSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDE 284

Query: 156 SLDGTGRIGVQLSPNVKISKVVAKNFLEAFSYSKKEFLGLSYNVLDSLKQTFLNFSQSAS 215
           +    GR G        +   VA         +K     LS  V    K   L       
Sbjct: 285 NKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVTGQFKLDML------- 344

Query: 216 KVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRG 275
             SGPV I  +  +VA++ I  L+QFAA L+INL ++NLLP+PALDGG L  + IEA+R 
Sbjct: 345 --SGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIR- 404

Query: 276 GRKLPLELEQRIMSSGIMFVVLLGLFLIIKDTLNL 307
           G+ +  E E  ++  G+ F++LL L +   D   L
Sbjct: 405 GKPINREKEAFVVFIGVAFLMLLMLVVTWNDIQRL 420

BLAST of Spg000388 vs. ExPASy TrEMBL
Match: A0A0A0LT84 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.3e-154
Identity = 293/310 (94.52%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 147 YSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 206

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELPKLGSTAVS+LVEA
Sbjct: 207 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEA 266

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 267 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 326

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 327 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 386

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 387 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 446

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 447 LNLEFIKDLL 456

BLAST of Spg000388 vs. ExPASy TrEMBL
Match: A0A5A7T039 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold213G00770 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.6e-154
Identity = 293/310 (94.52%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 59  YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 118

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELP LGSTAVS+LVEA
Sbjct: 119 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEA 178

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 179 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 238

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 239 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 298

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 299 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 358

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 359 LNLEFIKDLL 368

BLAST of Spg000388 vs. ExPASy TrEMBL
Match: A0A5D3E082 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00250 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.6e-154
Identity = 293/310 (94.52%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 148 YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 207

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELP LGSTAVS+LVEA
Sbjct: 208 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEA 267

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 268 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 327

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 328 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 387

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 388 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 447

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 448 LNLEFIKDLL 457

BLAST of Spg000388 vs. ExPASy TrEMBL
Match: A0A1S3C376 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495966 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.6e-154
Identity = 293/310 (94.52%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRV+VISAGVIANIIFAYIIIL+
Sbjct: 148 YSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILV 207

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLPGDVILAVNGNELP LGSTAVS+LVEA
Sbjct: 208 QVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEA 267

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG +DLEIGVTPD+S DGTGRIGVQLSPNVKISKVVAKNFLEAF+Y
Sbjct: 268 IKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNY 327

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 328 SRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 387

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSG+MFVVLLGLFLII+DT
Sbjct: 388 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDT 447

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 448 LNLEFIKDLL 457

BLAST of Spg000388 vs. ExPASy TrEMBL
Match: A0A6J1FWS5 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448141 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.1e-152
Identity = 291/310 (93.87%), Postives = 304/310 (98.06%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIP+DDENLLKNRPILDRV+VISAGVIANIIFAYIIILI
Sbjct: 140 YSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILI 199

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA
Sbjct: 200 QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 259

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           IKRSPNRTVLLK+ERG++DLEIGVTPDES DGTGRIGVQLSPNVKISK+VAKNFLEAFSY
Sbjct: 260 IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSY 319

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           S+KEFLGLSYNVLDSLKQT LN SQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI
Sbjct: 320 SRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 379

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGGSLALILIEA RGGRKLPLELEQRIMSSGIM VVLLG+FLI++DT
Sbjct: 380 NLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDT 439

Query: 304 LNLEFIKDLL 314
           LNLEFIKDLL
Sbjct: 440 LNLEFIKDLL 449

BLAST of Spg000388 vs. TAIR 10
Match: AT1G05140.1 (Peptidase M50 family protein )

HSP 1 Score: 464.2 bits (1193), Expect = 8.6e-131
Identity = 239/310 (77.10%), Postives = 276/310 (89.03%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+SDIPVDD NLLKNRPILDRVIV+SAG++AN+IFAY II  
Sbjct: 132 YSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRPILDRVIVVSAGIVANVIFAYAIIFT 191

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPGDVILAV+G EL   GS +VS++V+ 
Sbjct: 192 QVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPGDVILAVDGTELSNSGSDSVSKVVDV 251

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           +KR+P   VLL+IERGKE  EI +TPD+S DGTG+IGVQLSPNV+  KV  KN  E FS+
Sbjct: 252 VKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGKIGVQLSPNVRFGKVRPKNIPETFSF 311

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
           + +EF GLSYNVLDSLKQTFLNFSQ+ASKV+GPVAIIAVGAEVARSN DGLYQFAA+LN+
Sbjct: 312 AGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVAIIAVGAEVARSNADGLYQFAALLNL 371

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGG+LALIL+EAVRGGRKLPLE+EQ IMSSGIM V+ LGLFLI+KDT
Sbjct: 372 NLAVINLLPLPALDGGTLALILLEAVRGGRKLPLEVEQGIMSSGIMLVLFLGLFLIVKDT 431

Query: 304 LNLEFIKDLL 314
           LNL+FIK++L
Sbjct: 432 LNLDFIKEML 441

BLAST of Spg000388 vs. TAIR 10
Match: AT2G32480.1 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 448.7 bits (1153), Expect = 3.7e-126
Identity = 231/310 (74.52%), Postives = 273/310 (88.06%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+S+IP+DDENLLKNRP LDR IV+SAG+IAN+IFAY II +
Sbjct: 138 YSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFV 197

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVILAV+G EL K G  AVS++V+ 
Sbjct: 198 QVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDI 257

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           +KR+P   V+ +IERG ED +I VTPD++ DGTG+IGVQLSPNV+I+KV  +N  E F +
Sbjct: 258 VKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRF 317

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
             +EF+GLS NVLD LKQTF NFSQ+ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNI
Sbjct: 318 VGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNI 377

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGG+LALIL+EAVRGG+KLP+E+EQ IMSSGIM V+ LGLFLI+KDT
Sbjct: 378 NLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDT 437

Query: 304 LNLEFIKDLL 314
           L+L+FIK++L
Sbjct: 438 LSLDFIKEML 447

BLAST of Spg000388 vs. TAIR 10
Match: AT2G32480.2 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 389.8 bits (1000), Expect = 2.1e-108
Identity = 209/310 (67.42%), Postives = 247/310 (79.68%), Query Frame = 0

Query: 4   HQVRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVIVISAGVIANIIFAYIIILI 63
           + +RAFPLGGFVGFPDNDP+S+IP+DDENLLKNRP LDR IV+SAG+IAN+IFAY II +
Sbjct: 138 YSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFV 197

Query: 64  QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEA 123
           QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVILAV+G EL K G  AVS++V+ 
Sbjct: 198 QVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDI 257

Query: 124 IKRSPNRTVLLKIERGKEDLEIGVTPDESLDGTGRIGVQLSPNVKISKVVAKNFLEAFSY 183
           +KR+P   V+ +IERG ED +I VTPD++ DGTG+IGVQLSPNV+I+K            
Sbjct: 258 VKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGVQLSPNVRITK------------ 317

Query: 184 SKKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNI 243
                                    +ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNI
Sbjct: 318 -------------------------TASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNI 377

Query: 244 NLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGIMFVVLLGLFLIIKDT 303
           NLAVINLLPLPALDGG+LALIL+EAVRGG+KLP+E+EQ IMSSGIM V+ LGLFLI+KDT
Sbjct: 378 NLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDT 410

Query: 304 LNLEFIKDLL 314
           L+L+FIK++L
Sbjct: 438 LSLDFIKEML 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902635.11.6e-15595.48probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida][more]
XP_004151933.26.8e-15494.52probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus][more]
XP_008455887.11.2e-15394.52PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo... [more]
KAA0034835.11.2e-15394.52putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa][more]
KAG6570888.14.4e-15394.19Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
O230531.2e-12977.10Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana ... [more]
O808855.2e-12574.52Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P737141.0e-7246.45Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Ka... [more]
Q8YQ646.7e-6446.91Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82... [more]
O317549.5e-2633.09Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 G... [more]
Match NameE-valueIdentityDescription
A0A0A0LT843.3e-15494.52PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV... [more]
A0A5A7T0395.6e-15494.52Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3E0825.6e-15494.52Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3C3765.6e-15494.52probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 ... [more]
A0A6J1FWS51.1e-15293.87probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT1G05140.18.6e-13177.10Peptidase M50 family protein [more]
AT2G32480.13.7e-12674.52ARABIDOPSIS SERIN PROTEASE [more]
AT2G32480.22.1e-10867.42ARABIDOPSIS SERIN PROTEASE [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001478PDZ domainSMARTSM00228pdz_newcoord: 66..140
e-value: 7.2E-11
score: 52.1
IPR001478PDZ domainPROSITEPS50106PDZcoord: 68..139
score: 11.400599
IPR008915Peptidase M50PFAMPF02163Peptidase_M50coord: 5..294
e-value: 8.6E-39
score: 133.3
IPR036034PDZ superfamilyGENE3D2.30.42.10coord: 68..164
e-value: 8.6E-16
score: 60.0
IPR036034PDZ superfamilySUPERFAMILY50156PDZ domain-likecoord: 66..151
IPR041489PDZ domain 6PFAMPF17820PDZ_6coord: 81..138
e-value: 7.2E-9
score: 35.4
IPR004387Peptidase M50, putative membrane-associated zinc metallopeptidasePANTHERPTHR42837REGULATOR OF SIGMA-E PROTEASE RSEPcoord: 5..313
NoneNo IPR availablePANTHERPTHR42837:SF4MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATEDcoord: 5..313
NoneNo IPR availableCDDcd00989PDZ_metalloproteasecoord: 66..149
e-value: 4.98221E-14
score: 64.1772

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000388.1Spg000388.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0005515 protein binding