Spg000355 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000355
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ubiquitinyl hydrolase 1
Location	scaffold8: 44122158 .. 44125984 (-)
RNA-Seq Expression	Spg000355
Synteny	Spg000355

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAATCAAAGGAGAACCCGCCGAATCGACCAGCGAGTTCCGAAACGCTGAAGACGATCCGATTTCTTCAAAGTCGAACTCCACCGCCGCCCCTCCTGCCCGATATTCGCGGCGGCGTATGCCCTTTCCGTGGAATACAATTCCGAGATCGCTTCCATCTCCAACGATTCTCGAAGGCTTACATTCGAAACGAGTTTAGGTTTGTTGTTTTTATCGTCTATTCAACCAATGCCACAGTTCTAATTCTGAATCTAGATTCAGAGTTCTTTTTGCTTCTGAAATAGGAATTTTCTAGATTTCGGATTTCGGATTTGAGATCTAAGACTTAGACGATGGCGAGCGAAGATTGCCGAGTTTATCACGAGAGGCAAAAGCTACAGTTCTGCCTCTTGCACACTCTCAATAACCTCTTTCAGGTACTTTCACTACCAGATCTTGAAAATTACGATTATCGGTGTAATTAAGATGCAGTTAAACGGAATTATGCTGTTTCTCCTCATTAAGAAAGAATTATGGTACACAGGATCTCAGAATTCGACTTATCGTCTCACTATTTCACGATTTGTACCATTCCTTCTGTATTTCCATGCCTTCTTACTATTATATCTTTTATTAACATCGTAAATACCCCTTACAACAGGAAGAAGATGCATTTACTCGAGCGAACTTAAATGCAATTGCTGAAAAGCTGGTGCTGGACGAGCCAAACAAAGAACCTTGGACACCATTATCAATTGTCTTTAAGCCTCACCACAACACAGTAACTGGAAATTACGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTACTCAACGTCTCAGTTAGAAGGTTTGGTGGCATCTGGAAAAGTAGGCACTGGGTTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGACCTACCCGTTCCTCATCCGTTTAAGGATACCAACGAAGTAAGACAATTTTTGGATCATGTGATCAGTGACAATGGGGAAATTTTGCTAGTTATGAATGACAGGAACTGAATCTCTTCCAATAGGATTCTTGTGTTTGCGCTCAAATCAAATGTAAAATAAGAATTATAGCTGGATTGTGTTAGCTGTTTATCTGCAACTTCTTTTGTATACTGAGATGTACTTGGCTTGAGTTATGTTTCTGATTATTTCATTTTGCTGATAAAGAAGGGCAGTGATTTGAAGTGCAAAGCAATGAGAAACGGTTCATGTCTAAAATCAAACCCGAGTATTTTGTACCTAAAAGCCAAATGAAGACTGTTATCTGAAGTTGGTATCCTGAAGGAAGTCTGAATTTGCTAAGCTGCTGATAAAACTGCCATCAGATGAAACCGCGTCGGGTATTTTATAGAGCAGAATGGTTTGTTATGTTTAAAAAGGAATCAATAGTACAGGCCATAGAATGCCACACCAACAAACTTTGCTGGTTAAGGGCTCTACCGGCATCAGAGGTTAGCTTCAACTCTCATTGTGAGTTCTGAGCAAAAGTTTAAATTATCTCATCACCACCCATACTATTAGATTACATGGGCTTCCATCTCAAAACCAATTGGCAATGAGTGGGGTGACCCTTCTAACATATAAATTGTGAGGTCTCCACTTTTCTTTTCAATGTGGGATCCTCAACATGCCCCCTCAAGATGGTGCCTCTTTGGGTTCACCATTCTTGGATCGGATCCCAATTTCTTTTTATTGGACCGAATACCAGATCCGATTTCTTTTTATTGGACCGAATACCCGTTTGGGTTTAATGGGCTCTGATACCATATTAGATTACATGGGCTTCCATCTCAAAACCAATTGGCAATGAGTGGGGTGACCCTTCTAACATATAAATTGTGAGGTCTCCACTTTTCTTTTCAATGTGGGATCCTCAACACATACACCATTATCATCATTGCTACGAACACAAACGCAGTCTTTTTGTCTGATGAAAGCCTATATTATATATTTTAATAAGAAGTATGATGCATCACCACCAAAGAATGCTTCCGAAAGTTTGTTAAAATAAGAACGACTTTATGTACTCAGTAGATGTATTTCCCTATAATTATCTGCTCAAAGTCCTTTTCTTCTTAAATAGACATTTTAGCTTTAGAAATTCCCTTTTTCCTTTATCTGAAACGAGAATCTTTTGAGCCTTTCATCATTTCTTTTTCTCTGTCTTCCCCTTATTTAATATTGACTTTGCTTCCTTTTGGATTCATTAGTCGTTTTCCACTATTGCTTATGCTCCAAACTTGTGAAGGTTAGAGAAAGAATTGTTTTTTCAGAAAAGGAAAAGAAGAAGAAAAAGAATTTGGAAAAGATAGACAAAAAAAAGTCTGTATTCCCAGGCATATTCTCTCTCCTAAACTTAGACATTTAAAGTGCTATATTGGAGTCAATCTACCTTGCTAGCTATATTCAATAGTTTAACATTGCCAGCAATTTAGTCCACTAAAATGCAGTAGAGAGGACGAAATGATTATTGCTATATTTATGGTTTTATTTATGATTTGATGTCAAAATGTTGATTTCTACCATTTCTGTACCTACTTCATGCGTGCAAACTTTCTATATTCTCTGTTTATCTTGACATCCGGATAAAGTTAAACATGTCTTTGGCTGTTGGATCAAATATTTAACGGTTGAGATTGTATGATTAAAAAAGTGTTAGGCATTATATTGAATGACACCCTCACTTTGTTCGTTGCTTGATAGAGATTTAATGCACAAAAACAACAGAGCCTTCTTGGGTTTGCTTAAGAGAACGCTAGGTGGGCAGGCTGCATTTTTCAGTTGTGTGTACGTCTCTTTTCTCATCTCTCTTTTTCTCCTTTTCTCTTTGCCTTCCCTTCTATTCGCTATTTCAATTCATTTTAATCTCTCTCTCATCAACTTCTCTCTTTCTCTATCTAGTCTCAGACCTTTCTTCTAATCCCATGACGACAACAGTGGAGCAAAAGATTGAGAAACAGAACTCCTCTAGCAACAGAACTGCTCGAAACAGAGAGTCTTTTGGAAAGTGCAGATTTAGATCCCTTAAAGGGCTGCAATTCTCTCCGTTGAGCTTTCTGAAGCACCTGGCCGATAAAGTGACCCGAGCTTTGCATTTGATGGCCATGAAGAGAAGACCCCGCCACGAACAACCCGCTCCAGCCGTGCCCGTCGCCAGTTCAAAGCCACTGCCGGTCTCCGCCGTCGATTCACATAGAACAGAGGCTGTGGAGGACTGCATCAAGTACATCAACTCTTCGTCTTAGCTCACTCACACCAATTCTTGTAAGATTTTCCCCAGAAAATTCGTATATATGTCGTGTCCTTGTGGGTTTAATGTCTGATATCATTGTTGTGAATCATGATGCAATGCCTGTTTTTTCAGTCTTCTTTTAGACGAAATATTGGCGGGGAATGTTATCAATTATCATGATTCTTAGGCCAGTAGGTGGAATAAGCCTATCAATATAATAATATTTGTTCAAGAAGATCTATTGCGGATTGGAAAAACTTCCTGTCAATTTTCCATTATTTTCTTTTTGTGTCATTTTATGTCATTTTATCAAAATATTCATTCCATGTAGAAGATGATATTGAAGATTTTGCTTTTTTTTTTTTTCGGTGGAGAGATTCGAACCACAGACCTCTTGGTCATCAGTATAGTTTTATACATGTTGAGTTGTGCTCACGTTGGCTTGTTCATTTTTGTTTGTGGGTCCGTTTAAGTTCTCTTTTGTTGAAATCCAA

mRNA sequence

ATGGCGAGCGAAGATTGCCGAGTTTATCACGAGAGGCAAAAGCTACAGTTCTGCCTCTTGCACACTCTCAATAACCTCTTTCAGGATCTCAGAATTCGACTTATCGAAGAAGATGCATTTACTCGAGCGAACTTAAATGCAATTGCTGAAAAGCTGGTGCTGGACGAGCCAAACAAAGAACCTTGGACACCATTATCAATTGTCTTTAAGCCTCACCACAACACAGTAACTGGAAATTACGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTACTCAACGTCTCAGTTAGAAGGTTTGGTGGCATCTGGAAAAGTAGGCACTGGGTTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGACCTACCCGTTCCTCATCCGTTTAAGGATACCAACGAAGTAAGACAATTTTTGGATCATGTGATCAGTGACAATGGGGAAATTTTGCTAGTTATGAATGACAGGAACTGA

Coding sequence (CDS)

ATGGCGAGCGAAGATTGCCGAGTTTATCACGAGAGGCAAAAGCTACAGTTCTGCCTCTTGCACACTCTCAATAACCTCTTTCAGGATCTCAGAATTCGACTTATCGAAGAAGATGCATTTACTCGAGCGAACTTAAATGCAATTGCTGAAAAGCTGGTGCTGGACGAGCCAAACAAAGAACCTTGGACACCATTATCAATTGTCTTTAAGCCTCACCACAACACAGTAACTGGAAATTACGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAAGGGAAGAGTGTGGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGGCCTGAGGATACTCATAAACTTATGGGAATTGTACTCAACGTCTCAGTTAGAAGGTTTGGTGGCATCTGGAAAAGTAGGCACTGGGTTGCACTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGACCTACCCGTTCCTCATCCGTTTAAGGATACCAACGAAGTAAGACAATTTTTGGATCATGTGATCAGTGACAATGGGGAAATTTTGCTAGTTATGAATGACAGGAACTGA

Protein sequence

MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDNGEILLVMNDRN

Homology

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BLAST of Spg000355 vs. NCBI nr
Match: XP_038902688.1 (josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein [Benincasa hispida])

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-97
Identity = 173/191 (90.58%), Postives = 178/191 (93.19%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E+DAFTRANLNAIAEKLVLDEPN+E
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------EKDAFTRANLNAIAEKLVLDEPNEE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLS VFKPHHNTVTGNYDINVLISALEEKGK VVWHDHRNGASSIDLDGPEDTHKLM
Sbjct: 61  RWTPLSFVFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLP PHPFKDT+EVRQFLDHVI +N
Sbjct: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNN 180

Query: 181 GEILLVMNDRN 192
           GEILL+MNDRN
Sbjct: 181 GEILLIMNDRN 184

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BLAST of Spg000355 vs. NCBI nr
Match: XP_022986948.1 (josephin-like protein [Cucurbita maxima])

HSP 1 Score: 355.5 bits (911), Expect = 2.8e-94
Identity = 170/191 (89.01%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E +AFTRA+LNAIAEKLVLDEPNKE
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENNAFTRAHLNAIAEKLVLDEPNKE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHN+VTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPE THKLM
Sbjct: 61  PWTPLSFVFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNV VRRFGGIWKSRHWVALRKIN LWYNLDSDLP PH FKDT+EVRQFLD+VI+DN
Sbjct: 121 GIVLNVPVRRFGGIWKSRHWVALRKINELWYNLDSDLPTPHTFKDTDEVRQFLDNVINDN 180

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 181 GEILLVMNDRN 184

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BLAST of Spg000355 vs. NCBI nr
Match: XP_016903134.1 (PREDICTED: josephin-like protein [Cucumis melo] >XP_016903135.1 PREDICTED: josephin-like protein [Cucumis melo] >KAA0061848.1 josephin-like protein [Cucumis melo var. makuwa] >TYK15408.1 josephin-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 352.4 bits (903), Expect = 2.4e-93
Identity = 167/191 (87.43%), Postives = 174/191 (91.10%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           M  EDC VYHERQKLQ CLLH LNNLFQ       E+DAFTRANLNAIAEKLV DEPN+E
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQ-------EKDAFTRANLNAIAEKLVRDEPNQE 61

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGAS+IDLDGP+DT KLM
Sbjct: 62  TWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLM 121

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNVSVRRFGGIWKSRHWVALRKI+GLWYNLDSDLP PHPFKDT EVR+FLDHVI+DN
Sbjct: 122 GIVLNVSVRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDN 181

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 182 GEILLVMNDRN 185

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BLAST of Spg000355 vs. NCBI nr
Match: KAG6570667.1 (Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.7 bits (901), Expect = 4.1e-93
Identity = 168/191 (87.96%), Postives = 175/191 (91.62%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E DAFTRA+LNAI+EKLVLDEPN+E
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENDAFTRAHLNAISEKLVLDEPNRE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHN+VTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPE THKLM
Sbjct: 61  PWTPLSFVFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNV VRRFGGIWKSRHWVALRKIN LWYNLDSDL  PH FKDT+EVRQFLD+VI+DN
Sbjct: 121 GIVLNVPVRRFGGIWKSRHWVALRKINELWYNLDSDLHTPHTFKDTDEVRQFLDNVINDN 180

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 181 GEILLVMNDRN 184

[Close]

BLAST of Spg000355 vs. NCBI nr
Match: XP_023512266.1 (uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.7 bits (901), Expect = 4.1e-93
Identity = 168/191 (87.96%), Postives = 175/191 (91.62%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E DAFTRA+LNAI+EKLVLDEPN+E
Sbjct: 2   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENDAFTRAHLNAISEKLVLDEPNRE 61

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHN+VTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPE THKLM
Sbjct: 62  PWTPLSFVFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLM 121

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNV VRRFGGIWKSRHWVALRKIN LWYNLDSDL  PH FKDT+EVRQFLD+VI+DN
Sbjct: 122 GIVLNVPVRRFGGIWKSRHWVALRKINELWYNLDSDLHTPHTFKDTDEVRQFLDNVINDN 181

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 182 GEILLVMNDRN 185

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BLAST of Spg000355 vs. ExPASy Swiss-Prot
Match: O82391 (Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 3.0e-67
Identity = 120/178 (67.42%), Postives = 143/178 (80.34%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MA  + ++YHERQ+LQFCLLH LNNLFQD       +DAFT+ +LN+IAEKL  ++PNKE
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQD-------KDAFTKESLNSIAEKLETNDPNKE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLS V KPHHNT+TGNYD+NV+I+ALE KGKSVVWHD R GASSIDLD   D   LM
Sbjct: 61  TWTPLSFVLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVIS 179
           GIVLNV V+R+GG+W+SRHWV +RKING+WYNLDSDL VP  F+D +EVR FLD  +S
Sbjct: 121 GIVLNVPVKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLDQNLS 168

[Close]

BLAST of Spg000355 vs. ExPASy Swiss-Prot
Match: Q5EAE5 (Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-23
Identity = 66/188 (35.11%), Postives = 101/188 (53.72%), Query Frame = 0


Query: 7   RVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLS 66
           ++YHE+Q+ + C LH LNN+FQD        +AFTR  L  I ++L    PN        
Sbjct: 25  QIYHEKQRRELCALHALNNVFQD-------SNAFTRETLQEIFQRL---SPN-------- 84

Query: 67  IVFKPHHNTV--TGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVL 126
            +  PH  ++   GNYD+NV+++AL+ KG   VW D R    +I L        +MG ++
Sbjct: 85  TMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGAIALT------NVMGFIM 144

Query: 127 NVSVRRFGG----IWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVI-SD 186
           N+      G      K +HW+ +R++ G +YNLDS L +P      +E+R+FL H +   
Sbjct: 145 NLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGKSELRKFLKHHLRGK 188

Query: 187 NGEILLVM 188
           N E+LLV+
Sbjct: 205 NCELLLVV 188

[Close]

BLAST of Spg000355 vs. ExPASy Swiss-Prot
Match: Q5BJY4 (Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.1e-23
Identity = 67/188 (35.64%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 7   RVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLS 66
           ++YHE+Q+ + C LH LNN+FQD        +AFTR  L  I ++L    PN        
Sbjct: 25  QIYHEKQRRELCALHALNNVFQD-------SNAFTRETLQEIFQRL---SPN-------- 84

Query: 67  IVFKPHHNTV--TGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVL 126
            V  PH  ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++
Sbjct: 85  TVVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIAL------ANVMGFIM 144

Query: 127 NVSVRRFGG----IWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLD-HVISD 186
           N+      G      K +HW+ +R++ G +YNLDS L +P      +E+R+FL  H+   
Sbjct: 145 NLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGK 188

Query: 187 NGEILLVM 188
           N E+LLV+
Sbjct: 205 NCELLLVV 188

[Close]

BLAST of Spg000355 vs. ExPASy Swiss-Prot
Match: Q15040 (Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.6e-23
Identity = 66/188 (35.11%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 7   RVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLS 66
           ++YHE+Q+ + C LH LNN+FQD        +AFTR  L  I ++L    PN        
Sbjct: 25  QIYHEKQRRELCALHALNNVFQD-------SNAFTRDTLQEIFQRL---SPN-------- 84

Query: 67  IVFKPHHNTV--TGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVL 126
            +  PH  ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++
Sbjct: 85  TMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIM 144

Query: 127 NVSVRRFGG----IWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVI-SD 186
           N+      G      K +HW+ +R++ G +YNLDS L +P      +E+R+FL H +   
Sbjct: 145 NLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGK 188

Query: 187 NGEILLVM 188
           N E+LLV+
Sbjct: 205 NCELLLVV 188

[Close]

BLAST of Spg000355 vs. ExPASy Swiss-Prot
Match: Q9DBJ6 (Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.6e-23
Identity = 66/188 (35.11%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 7   RVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLS 66
           ++YHE+Q+ + C LH LNN+FQD        +AFTR  L  I ++L    PN        
Sbjct: 25  QIYHEKQRRELCALHALNNVFQD-------SNAFTRETLQEIFQRL---SPN-------- 84

Query: 67  IVFKPHHNTV--TGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVL 126
            +  PH  ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++
Sbjct: 85  TMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIM 144

Query: 127 NVSVRRFGG----IWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLD-HVISD 186
           N+      G      K +HW+ +R++ G +YNLDS L +P      +E+R+FL  H+   
Sbjct: 145 NLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGK 188

Query: 187 NGEILLVM 188
           N E+LLV+
Sbjct: 205 NCELLLVV 188

[Close]

BLAST of Spg000355 vs. ExPASy TrEMBL
Match: A0A6J1JI12 (Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.4e-94
Identity = 170/191 (89.01%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E +AFTRA+LNAIAEKLVLDEPNKE
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENNAFTRAHLNAIAEKLVLDEPNKE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHN+VTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPE THKLM
Sbjct: 61  PWTPLSFVFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNV VRRFGGIWKSRHWVALRKIN LWYNLDSDLP PH FKDT+EVRQFLD+VI+DN
Sbjct: 121 GIVLNVPVRRFGGIWKSRHWVALRKINELWYNLDSDLPTPHTFKDTDEVRQFLDNVINDN 180

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 181 GEILLVMNDRN 184

[Close]

BLAST of Spg000355 vs. ExPASy TrEMBL
Match: A0A1S4E4I3 (Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 167/191 (87.43%), Postives = 174/191 (91.10%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           M  EDC VYHERQKLQ CLLH LNNLFQ       E+DAFTRANLNAIAEKLV DEPN+E
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQ-------EKDAFTRANLNAIAEKLVRDEPNQE 61

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGAS+IDLDGP+DT KLM
Sbjct: 62  TWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLM 121

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNVSVRRFGGIWKSRHWVALRKI+GLWYNLDSDLP PHPFKDT EVR+FLDHVI+DN
Sbjct: 122 GIVLNVSVRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDN 181

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 182 GEILLVMNDRN 185

[Close]

BLAST of Spg000355 vs. ExPASy TrEMBL
Match: A0A5D3CVY1 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00510 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 167/191 (87.43%), Postives = 174/191 (91.10%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           M  EDC VYHERQKLQ CLLH LNNLFQ       E+DAFTRANLNAIAEKLV DEPN+E
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQ-------EKDAFTRANLNAIAEKLVRDEPNQE 61

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGAS+IDLDGP+DT KLM
Sbjct: 62  TWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLM 121

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNVSVRRFGGIWKSRHWVALRKI+GLWYNLDSDLP PHPFKDT EVR+FLDHVI+DN
Sbjct: 122 GIVLNVSVRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDN 181

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 182 GEILLVMNDRN 185

[Close]

BLAST of Spg000355 vs. ExPASy TrEMBL
Match: A0A6J1D2P2 (Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111017028 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.5e-93
Identity = 167/191 (87.43%), Postives = 173/191 (90.58%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E DAFTRANLNAIAE LVL+EPNKE
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENDAFTRANLNAIAENLVLNEPNKE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHNTVTGNYDINVLISALEEKGKSVVWHD RNGASSIDLDGPEDTHKLM
Sbjct: 61  PWTPLSFVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNVSVRRFGGIWKSRHW+ALRKINGLWYNLDSDLP P PFKDT+EVR+FLD+ I  +
Sbjct: 121 GIVLNVSVRRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHD 180

Query: 181 GEILLVMNDRN 192
           GEILLVMND N
Sbjct: 181 GEILLVMNDGN 184

[Close]

BLAST of Spg000355 vs. ExPASy TrEMBL
Match: A0A6J1FYW5 (Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.7e-92
Identity = 166/191 (86.91%), Postives = 174/191 (91.10%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MASEDCRVYHERQKLQFCLLH LNNLFQ       E DAFTRA+LNAI+EKLVLDEPN+E
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQ-------ENDAFTRAHLNAISEKLVLDEPNRE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
           PWTPLS VFKPHHN+VTGNYDINVLISA+EEKGKSVVWHDHRNGASSIDLDGPE THKLM
Sbjct: 61  PWTPLSFVFKPHHNSVTGNYDINVLISAMEEKGKSVVWHDHRNGASSIDLDGPEATHKLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVISDN 180
           GIVLNV VRRFGGIWKSRHWVALRKIN  WYNLDSDL  PH FKDT+EVRQFLD+VI+DN
Sbjct: 121 GIVLNVPVRRFGGIWKSRHWVALRKINESWYNLDSDLHTPHTFKDTDEVRQFLDNVINDN 180

Query: 181 GEILLVMNDRN 192
           GEILLVMNDRN
Sbjct: 181 GEILLVMNDRN 184

[Close]

BLAST of Spg000355 vs. TAIR 10
Match: AT2G29640.1 (JOSEPHIN-like protein )

HSP 1 Score: 256.1 bits (653), Expect = 2.2e-68
Identity = 120/178 (67.42%), Postives = 143/178 (80.34%), Query Frame = 0


Query: 1   MASEDCRVYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKE 60
           MA  + ++YHERQ+LQFCLLH LNNLFQD       +DAFT+ +LN+IAEKL  ++PNKE
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQD-------KDAFTKESLNSIAEKLETNDPNKE 60

Query: 61  PWTPLSIVFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLM 120
            WTPLS V KPHHNT+TGNYD+NV+I+ALE KGKSVVWHD R GASSIDLD   D   LM
Sbjct: 61  TWTPLSFVLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLM 120

Query: 121 GIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVPHPFKDTNEVRQFLDHVIS 179
           GIVLNV V+R+GG+W+SRHWV +RKING+WYNLDSDL VP  F+D +EVR FLD  +S
Sbjct: 121 GIVLNVPVKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLDQNLS 168

[Close]

BLAST of Spg000355 vs. TAIR 10
Match: AT3G54130.1 (Josephin family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.2e-05
Identity = 39/154 (25.32%), Postives = 65/154 (42.21%), Query Frame = 0


Query: 8   VYHERQKLQFCLLHTLNNLFQDLRIRLIEEDAFTRANLNAIAEKLVLDEPNKEPWTPLSI 67
           +YHE Q+   C +H +N + Q       +  A   A+L+    +++L+      + P   
Sbjct: 10  LYHEVQESNLCAVHCVNTVLQGPFFSEFDL-AAVAADLDGKERQVMLEGAAVGGFAPGDF 69

Query: 68  VFKPHHN-TVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEDTHKLMGIVLNV 127
           + +  HN ++ G++ I VL  ALE      VW       +  D +  +   +L    +  
Sbjct: 70  LAEESHNVSLGGDFSIQVLQKALE------VWDLQVIPLNCPDAEPAQIDPELESAFI-- 129

Query: 128 SVRRFGGIWKSRHWVALRKINGLWYNLDSDLPVP 161
                       HW  +RK+NG WYN DS L  P
Sbjct: 130 -------CHLHDHWFCIRKVNGEWYNFDSLLAAP 147

The following BLAST results are available for this feature:

BLAST of Spg000355 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902688.1	3.5e-97	90.58	josephin-like protein [Benincasa hispida] >XP_038902689.1 josephin-like protein ...	[more]
XP_022986948.1	2.8e-94	89.01	josephin-like protein [Cucurbita maxima]	[more]
XP_016903134.1	2.4e-93	87.43	PREDICTED: josephin-like protein [Cucumis melo] >XP_016903135.1 PREDICTED: josep...	[more]
KAG6570667.1	4.1e-93	87.96	Josephin-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023512266.1	4.1e-93	87.96	uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg000355 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82391	3.0e-67	67.42	Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1	[more]
Q5EAE5	1.6e-23	35.11	Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1	[more]
Q5BJY4	2.1e-23	35.64	Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1	[more]
Q15040	4.6e-23	35.11	Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1	[more]
Q9DBJ6	4.6e-23	35.11	Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1	[more]

BLAST of Spg000355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JI12	1.4e-94	89.01	Ubiquitinyl hydrolase 1 OS=Cucurbita maxima OX=3661 GN=LOC111484533 PE=4 SV=1	[more]
A0A1S4E4I3	1.2e-93	87.43	Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1	[more]
A0A5D3CVY1	1.2e-93	87.43	Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1D2P2	7.5e-93	87.43	Ubiquitinyl hydrolase 1 OS=Momordica charantia OX=3673 GN=LOC111017028 PE=4 SV=1	[more]
A0A6J1FYW5	1.7e-92	86.91	Ubiquitinyl hydrolase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449101 PE=4 SV=1	[more]

BLAST of Spg000355 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29640.1	2.2e-68	67.42	JOSEPHIN-like protein	[more]
AT3G54130.1	1.2e-05	25.32	Josephin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006155	Josephin domain	SMART	SM01246	Josephin_2	coord: 12..189 e-value: 4.6E-62 score: 222.2
IPR006155	Josephin domain	PFAM	PF02099	Josephin	coord: 13..186 e-value: 8.1E-34 score: 117.0
IPR006155	Josephin domain	PROSITE	PS50957	JOSEPHIN	coord: 5..191 score: 44.384281
None	No IPR available	GENE3D	3.90.70.40		coord: 17..191 e-value: 5.0E-25 score: 89.7
None	No IPR available	PANTHER	PTHR13291:SF0	JOSEPHIN-LIKE PROTEIN	coord: 1..190
IPR040053	Josephin-1/2	PANTHER	PTHR13291	JOSEPHIN 1, 2	coord: 1..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000355.1	Spg000355.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
molecular_function	GO:0004843	thiol-dependent deubiquitinase