Spg000176 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000176
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDUF4228 domain protein
Locationscaffold6: 22831777 .. 22833193 (+)
RNA-Seq ExpressionSpg000176
SyntenySpg000176
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCATGAAGCGAACCCCGAAGGAGGGTGAGTCGAAGGGATTGGGTCGTCTCGGCCCACTCGTGTGGGCCGAGTCTTCTCCCCTTCGCTCGATCTCTGGTGCCCCTAGATGCCCCGGTTCCACCTAGTTCAACCCAAATCGTCTCCGAAAGCCTAGAAACCCTAAGGCAGGAAAATGTATTTAAACTCTTCTCGTCACTGAAGAAGGGATCCCGAAACTATTTCTTTGAACTTTACTTCTCTTGCTCTCATGCTCTCCTCCTTCAGCTTTCTGACTTAAGCATCGGAGGTGGTGTGACAAGTACCACACTGGTATGCAGGTTTCTTCTATCTTGTCGGCCACGTCTTCCCCCTCTTAAAAAATATTCACGGTTGGTGTCACGTGAAGGTCAGGTGAGTCCTGCCTGTCCAAATTTGACCATCAACAACTATAATTATTAAAAAAAAAAAAAAAACAATCTAAACTTCACTATTCTTATTAAAAATAATACAATATATTATTGCTATATATTATTATAATGGAGTCAAAGAATCAAACATTTTGGCGTTCTGTGGTTCATGAATTAGAATGATGTTGAAAATTCCACCATATATTTGTCTAATACAATATTTAGCTTTACTTATCTAAATTATTAAGCCAACTGATCTGTGTTATACTCCAATAAAATTATGTTAGTTTTTGAAAGATTGCAACTATTTTTTCACCATAAACAATTAAGTTTCGCAAAATCTAAAATATACATCAAATACATAGGTTAAGCAAACTCAATTTTTTTTAGATACATCTTTTTTTATAAATTCAAACAAAAATCACACAAATTCAGACTTGTTAATTTAAACTCGCTTATAATGAATAAGAAAAAGGAGTTTGTTGGAAGGGTGATTTTATTAGGAGATAAATTTCAGTCAAACGTTTTTCTTTGAGTTTTCAATAATTCCACGTGATAATTAAAGAGACGCTTTGGCTTGACTATTCAATTTACGCCAACAACGTAACCTTCACAATTTCCTCCAAATATTTATATATAACTTCATCCTTTCTCCTTTCTCCAAAACTCTCTCTCTCTCTCCCTTTCTGTCTCAGCAGATTTTATCATCTTGGCCATGGGCAATTGTACGAGCAACAAATATTTGGCAGATACAATGCCGATGAGCGACGACGACGAGGAGGCGTCGGCGGAGACGGTGAAGAGGAGGCTGATCGACAACGGAGCTCGGAAAACTTCGATGGGAACGACGGAGTTGAAGATCAGAATCACGAAGAGAGAGCTGGAGAAGTTATTGAGCCAAGTGGATGTGGAGGAGCTGCCGGTGACGGAGCTGTTATCGCAGTTAATCGACCTTGGCGACGCATTTGAGAGTACTCACCGGCGATCATGGCGGCCTTCTTTGCACAGCATTCCTGAGGTCAATTGA

mRNA sequence

ATGATCATGAAGCGAACCCCGAAGGAGGATGCCCCGGTTCCACCTAGTTCAACCCAAATCGTCTCCGAAAGCCTAGAAACCCTAAGGCAGGAAAATGTATTTAAACTCTTCTCGTCACTGAAGAAGGGATCCCGAAACTATTTCTTTGAACTTTACTTCTCTTGCTCTCATGCTCTCCTCCTTCAGCTTTCTGACTTAAGCATCGGAGGTGGTGTGACAAGTACCACACTGGTATGCAGCAACAAATATTTGGCAGATACAATGCCGATGAGCGACGACGACGAGGAGGCGTCGGCGGAGACGGTGAAGAGGAGGCTGATCGACAACGGAGCTCGGAAAACTTCGATGGGAACGACGGAGTTGAAGATCAGAATCACGAAGAGAGAGCTGGAGAAGTTATTGAGCCAAGTGGATGTGGAGGAGCTGCCGGTGACGGAGCTGTTATCGCAGTTAATCGACCTTGGCGACGCATTTGAGAGTACTCACCGGCGATCATGGCGGCCTTCTTTGCACAGCATTCCTGAGGTCAATTGA

Coding sequence (CDS)

ATGATCATGAAGCGAACCCCGAAGGAGGATGCCCCGGTTCCACCTAGTTCAACCCAAATCGTCTCCGAAAGCCTAGAAACCCTAAGGCAGGAAAATGTATTTAAACTCTTCTCGTCACTGAAGAAGGGATCCCGAAACTATTTCTTTGAACTTTACTTCTCTTGCTCTCATGCTCTCCTCCTTCAGCTTTCTGACTTAAGCATCGGAGGTGGTGTGACAAGTACCACACTGGTATGCAGCAACAAATATTTGGCAGATACAATGCCGATGAGCGACGACGACGAGGAGGCGTCGGCGGAGACGGTGAAGAGGAGGCTGATCGACAACGGAGCTCGGAAAACTTCGATGGGAACGACGGAGTTGAAGATCAGAATCACGAAGAGAGAGCTGGAGAAGTTATTGAGCCAAGTGGATGTGGAGGAGCTGCCGGTGACGGAGCTGTTATCGCAGTTAATCGACCTTGGCGACGCATTTGAGAGTACTCACCGGCGATCATGGCGGCCTTCTTTGCACAGCATTCCTGAGGTCAATTGA

Protein sequence

MIMKRTPKEDAPVPPSSTQIVSESLETLRQENVFKLFSSLKKGSRNYFFELYFSCSHALLLQLSDLSIGGGVTSTTLVCSNKYLADTMPMSDDDEEASAETVKRRLIDNGARKTSMGTTELKIRITKRELEKLLSQVDVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN
Homology
BLAST of Spg000176 vs. NCBI nr
Match: XP_038904231.1 (uncharacterized protein LOC120090574 [Benincasa hispida])

HSP 1 Score: 150.2 bits (378), Expect = 1.7e-32
Identity = 83/100 (83.00%), Postives = 91/100 (91.00%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRL-IDNGARKTSMG-TTELKIRITKRELEKLLSQV 139
           SNKYLA+TMPMSD+DEEA  ETV+RRL + +GARK S   TTELKIRITKRELEKLLS+V
Sbjct: 6   SNKYLAETMPMSDEDEEA-VETVRRRLVVHDGARKCSANTTTELKIRITKRELEKLLSKV 65

Query: 140 DVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           DVEELPVTELLSQLID+GDAFESTH+RSWRPSL SIPEVN
Sbjct: 66  DVEELPVTELLSQLIDVGDAFESTHQRSWRPSLQSIPEVN 104

BLAST of Spg000176 vs. NCBI nr
Match: XP_031739098.1 (uncharacterized protein LOC116402843 [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-28
Identity = 74/100 (74.00%), Postives = 84/100 (84.00%), Query Frame = 0

Query: 80  SNKYLADT-MPMSDDDEEASAETVKRRLIDNGARKTSMG-TTELKIRITKRELEKLLSQV 139
           SNKYLA+T +PMSDDDEE      +R ++ NGA+K S   TTELKIRITKRELEKLL +V
Sbjct: 6   SNKYLAETIVPMSDDDEEVEEMLRRRLVVKNGAQKCSASTTTELKIRITKRELEKLLCKV 65

Query: 140 DVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           DVEELPVTELLS+LID+GD FESTH+RSWRPSL SIPEVN
Sbjct: 66  DVEELPVTELLSKLIDVGDTFESTHQRSWRPSLQSIPEVN 105

BLAST of Spg000176 vs. NCBI nr
Match: XP_008463409.1 (PREDICTED: uncharacterized protein LOC103501580 [Cucumis melo] >TYK02554.1 DUF4228 domain protein [Cucumis melo var. makuwa])

HSP 1 Score: 134.4 bits (337), Expect = 9.4e-28
Identity = 70/99 (70.71%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRLIDNGARKTSMG-TTELKIRITKRELEKLLSQVD 139
           SNKYLA+TMPMSDD+EE      +R ++++ A+K S   TTELKIRITK+ELEKLL +VD
Sbjct: 6   SNKYLAETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEKLLCKVD 65

Query: 140 VEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           +EELPV ELLS+LID+GDAFESTHRRSW+PSL SIPEVN
Sbjct: 66  MEELPVAELLSKLIDVGDAFESTHRRSWQPSLQSIPEVN 104

BLAST of Spg000176 vs. NCBI nr
Match: XP_022151029.1 (uncharacterized protein LOC111019048 [Momordica charantia])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 75/104 (72.12%), Postives = 85/104 (81.73%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRLI--DNG---ARKTSMG-TTELKIRITKRELEKL 139
           SNKY A+  PMS  DEE + ETVKRRLI  D+G    R +S+  TTE+KIRITKRELEKL
Sbjct: 6   SNKYSAEISPMS--DEEEAVETVKRRLIPDDDGHGALRSSSLARTTEVKIRITKRELEKL 65

Query: 140 LSQVDVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           L +VDVEELPVTELLSQLID+GDAFESTHRRSWRPSL +I E+N
Sbjct: 66  LRKVDVEELPVTELLSQLIDVGDAFESTHRRSWRPSLQTIHELN 107

BLAST of Spg000176 vs. NCBI nr
Match: XP_022985276.1 (uncharacterized protein LOC111483315 [Cucurbita maxima])

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-25
Identity = 71/102 (69.61%), Postives = 85/102 (83.33%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRL-IDNGARKTSMG----TTELKIRITKRELEKLL 139
           SNKYLA+TMPMS  +EE   ET+KR+L  D+GA ++S G    TTE+KIR+TKRELEKLL
Sbjct: 6   SNKYLAETMPMS--EEEELVETLKRKLNADDGASRSSRGTTTTTTEVKIRMTKRELEKLL 65

Query: 140 SQVDVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEV 177
           S+V+VEELPVTELL QLI++GD  ESTHRRSWRPSL SIPE+
Sbjct: 66  SKVEVEELPVTELLLQLIEVGDESESTHRRSWRPSLQSIPEL 105

BLAST of Spg000176 vs. ExPASy TrEMBL
Match: A0A0A0L2D8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G076560 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.2e-28
Identity = 74/100 (74.00%), Postives = 84/100 (84.00%), Query Frame = 0

Query: 80  SNKYLADT-MPMSDDDEEASAETVKRRLIDNGARKTSMG-TTELKIRITKRELEKLLSQV 139
           SNKYLA+T +PMSDDDEE      +R ++ NGA+K S   TTELKIRITKRELEKLL +V
Sbjct: 6   SNKYLAETIVPMSDDDEEVEEMLRRRLVVKNGAQKCSASTTTELKIRITKRELEKLLCKV 65

Query: 140 DVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           DVEELPVTELLS+LID+GD FESTH+RSWRPSL SIPEVN
Sbjct: 66  DVEELPVTELLSKLIDVGDTFESTHQRSWRPSLQSIPEVN 105

BLAST of Spg000176 vs. ExPASy TrEMBL
Match: A0A5D3BTQ9 (DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00050 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 4.6e-28
Identity = 70/99 (70.71%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRLIDNGARKTSMG-TTELKIRITKRELEKLLSQVD 139
           SNKYLA+TMPMSDD+EE      +R ++++ A+K S   TTELKIRITK+ELEKLL +VD
Sbjct: 6   SNKYLAETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEKLLCKVD 65

Query: 140 VEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           +EELPV ELLS+LID+GDAFESTHRRSW+PSL SIPEVN
Sbjct: 66  MEELPVAELLSKLIDVGDAFESTHRRSWQPSLQSIPEVN 104

BLAST of Spg000176 vs. ExPASy TrEMBL
Match: A0A1S3CJ77 (uncharacterized protein LOC103501580 OS=Cucumis melo OX=3656 GN=LOC103501580 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 4.6e-28
Identity = 70/99 (70.71%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRLIDNGARKTSMG-TTELKIRITKRELEKLLSQVD 139
           SNKYLA+TMPMSDD+EE      +R ++++ A+K S   TTELKIRITK+ELEKLL +VD
Sbjct: 6   SNKYLAETMPMSDDEEEVEEMLRRRLVVESRAQKCSASTTTELKIRITKKELEKLLCKVD 65

Query: 140 VEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           +EELPV ELLS+LID+GDAFESTHRRSW+PSL SIPEVN
Sbjct: 66  MEELPVAELLSKLIDVGDAFESTHRRSWQPSLQSIPEVN 104

BLAST of Spg000176 vs. ExPASy TrEMBL
Match: A0A6J1DBT0 (uncharacterized protein LOC111019048 OS=Momordica charantia OX=3673 GN=LOC111019048 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.6e-26
Identity = 75/104 (72.12%), Postives = 85/104 (81.73%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRLI--DNG---ARKTSMG-TTELKIRITKRELEKL 139
           SNKY A+  PMS  DEE + ETVKRRLI  D+G    R +S+  TTE+KIRITKRELEKL
Sbjct: 6   SNKYSAEISPMS--DEEEAVETVKRRLIPDDDGHGALRSSSLARTTEVKIRITKRELEKL 65

Query: 140 LSQVDVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
           L +VDVEELPVTELLSQLID+GDAFESTHRRSWRPSL +I E+N
Sbjct: 66  LRKVDVEELPVTELLSQLIDVGDAFESTHRRSWRPSLQTIHELN 107

BLAST of Spg000176 vs. ExPASy TrEMBL
Match: A0A6J1J7P7 (uncharacterized protein LOC111483315 OS=Cucurbita maxima OX=3661 GN=LOC111483315 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-25
Identity = 71/102 (69.61%), Postives = 85/102 (83.33%), Query Frame = 0

Query: 80  SNKYLADTMPMSDDDEEASAETVKRRL-IDNGARKTSMG----TTELKIRITKRELEKLL 139
           SNKYLA+TMPMS  +EE   ET+KR+L  D+GA ++S G    TTE+KIR+TKRELEKLL
Sbjct: 6   SNKYLAETMPMS--EEEELVETLKRKLNADDGASRSSRGTTTTTTEVKIRMTKRELEKLL 65

Query: 140 SQVDVEELPVTELLSQLIDLGDAFESTHRRSWRPSLHSIPEV 177
           S+V+VEELPVTELL QLI++GD  ESTHRRSWRPSL SIPE+
Sbjct: 66  SKVEVEELPVTELLLQLIEVGDESESTHRRSWRPSLQSIPEL 105

BLAST of Spg000176 vs. TAIR 10
Match: AT4G21920.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20340.1); Has 40 Blast hits to 40 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 40; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 50.1 bits (118), Expect = 2.2e-06
Identity = 33/94 (35.11%), Postives = 53/94 (56.38%), Query Frame = 0

Query: 91  SDDDEEASAETVKRRLIDNGARKTSMGTTELKIRITKRELEKLLSQVDVEELPVTELLSQ 150
           +DD E+   ET     + + +  +S    E+KIRITK+ELE L+  + ++ L   E+LS+
Sbjct: 39  NDDGEKLLGETSN---VTSTSSSSSSERREIKIRITKKELEDLMRNIGLKSLTAEEILSK 98

Query: 151 LI-DLGDAF------ESTHRRSWRPSLHSIPEVN 178
           LI + GD         + H + W+P L SIPE++
Sbjct: 99  LIFEGGDQIGFSAVDVTNHHQPWKPVLQSIPEMD 129

BLAST of Spg000176 vs. TAIR 10
Match: AT3G20340.1 (Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress. )

HSP 1 Score: 48.5 bits (114), Expect = 6.3e-06
Identity = 31/90 (34.44%), Postives = 54/90 (60.00%), Query Frame = 0

Query: 92  DDDEEASAETVK--RRLIDNGARKTSMGTTELKIRITKRELEKLLSQVDVEELPVTE--L 151
           ++D   S++T +  + +I     K+S+ + E+KIR+TK++L  LLS+V+V +L   +   
Sbjct: 26  EEDHHYSSKTTRDGKPVIVTRDSKSSVPSHEIKIRLTKKQLHDLLSKVNVHDLTFQQQTF 85

Query: 152 LSQLIDLGDAFESTHRRSWRPSLHSIPEVN 178
              +++     E+  +R WRP L SIPEVN
Sbjct: 86  SCPILNNRGYEEANQQRLWRPVLQSIPEVN 115

BLAST of Spg000176 vs. TAIR 10
Match: AT3G10120.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G03890.1); Has 57 Blast hits to 57 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 57; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 44.3 bits (103), Expect = 1.2e-04
Identity = 28/70 (40.00%), Postives = 38/70 (54.29%), Query Frame = 0

Query: 108 DNGARKTSMGTTELKIRITKRELEKLLSQVDVEELPVTELLSQLIDLGDAFESTHRRSWR 167
           DN   KT+ G   +K+ ++K+ELEKLL    V E+    L  Q   L D  +  H+  WR
Sbjct: 107 DNTKEKTN-GVVRVKMVVSKQELEKLLQGGSVHEMVYRTLAKQ--HLCDDDDECHKEGWR 166

Query: 168 PSLHSIPEVN 178
           P L SIPE +
Sbjct: 167 PLLDSIPETD 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904231.11.7e-3283.00uncharacterized protein LOC120090574 [Benincasa hispida][more]
XP_031739098.12.5e-2874.00uncharacterized protein LOC116402843 [Cucumis sativus][more]
XP_008463409.19.4e-2870.71PREDICTED: uncharacterized protein LOC103501580 [Cucumis melo] >TYK02554.1 DUF42... [more]
XP_022151029.11.2e-2572.12uncharacterized protein LOC111019048 [Momordica charantia][more]
XP_022985276.12.6e-2569.61uncharacterized protein LOC111483315 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L2D81.2e-2874.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G076560 PE=4 SV=1[more]
A0A5D3BTQ94.6e-2870.71DUF4228 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold9... [more]
A0A1S3CJ774.6e-2870.71uncharacterized protein LOC103501580 OS=Cucumis melo OX=3656 GN=LOC103501580 PE=... [more]
A0A6J1DBT05.6e-2672.12uncharacterized protein LOC111019048 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1J7P71.2e-2569.61uncharacterized protein LOC111483315 OS=Cucurbita maxima OX=3661 GN=LOC111483315... [more]
Match NameE-valueIdentityDescription
AT4G21920.12.2e-0635.11unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT3G20340.16.3e-0634.44Expression of the gene is downregulated in the presence of paraquat, an inducer ... [more]
AT3G10120.11.2e-0440.00unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33647OS01G0793900 PROTEINcoord: 90..176
NoneNo IPR availablePANTHERPTHR33647:SF5OS01G0793900 PROTEINcoord: 90..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000176.1Spg000176.1mRNA