Spg000138 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000138
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncalcium-dependent protein kinase-like
Locationscaffold6: 22058351 .. 22063157 (-)
RNA-Seq ExpressionSpg000138
SyntenySpg000138
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATCTGCACAAGCAAAGGAAAATACTCACCTCAGTATGATCGTTATGAACAGCAAGCAAAAGTCCCAGCTCAACACGACCACCGGTCGCCGGAAAATCACCATTCGGCGGCGGCGAAGTCAACGGGTGCTCCCAAAAGCCCCAGACACAACATTCTCAGGTCTGATACGATTCTTGGGAAGCCATTAGAGGATGTTAAGCTGCATTACACGATTGGGAAAGAATTGGGGAGAGGGCAATTTGGGGTTACTTATCTGTGCACTGAGAATTCCTCAGGTAAGCAATATGCTTGCAAGTCCATTTCGAAGAGGAAGCTTGTGACCAGAACTGATAAGGAGGATATCAGGAGGGAGATTCACATCATGCAGCATTTGAGTGGGCAGCCCAACATAGTTGAGTTCAAGGGAGCTTATGAAGACAAGCAATCGGTTCATTTGATCATGGAGCTTTGCGCTGGAGGCGAGCTGTTTGATAGAATTATTGCAAAGGGTCATTACAGTGAGAAAGCAGCTGCCTCAATCTGCAGGTCAATTGTTAATGTGGTTCATATCTGCCATTTCATGGGGGTGATGCACAGGGACCTCAAGCCTGAGAACTTTCTGCTTGCTAGCAAAGACGAAGATGCTATGCTCAAAGCAACAGATTTTGGATTGTCTGTTTTTATTGAAGATGGTGAGGTTCTTGCTCTGCTTATTGGATTTGTTCTTCATGCCTTTTTGATTGAGTGTCTTTCTGTGCTTGTTTTAGTTGACTTTGCTGTTCTTGTTAATGGTTTTAGAAGGTCAAATGTGTGAGAAATTGTGTGTGGCTTACAAGTTTTAGATGGTAGAACTAGATATATGTTTCTTTTTTTCTATATTTCATTTCTACAATTACTGTTGGTGATCATCTGTTGTAGTGTAGGCTAACGTTCAAAGTCTTGGGGCTTTTGTTTTTGGGAGAAATTCTTTTTCCATCCTGTATGTGGCTCAATGAGTCAATTTTAATTTTGAATATTCAGTTTCATCAAAGTTAACCCAAACTTAAACAAGTGGTGAAATTAATACCCTCAATTTGCTTGTGTTGGATACATTTTAGCTAGGTTTCGAATTTTATTGCTAAGTTTGGGCATCTTTTAACTTGTTTGATTTTGTAGTTGAGGGTATTTCCATAGTTGTGTTTGGACATTTTTGGCTTGATTTTGATATCTTTTGTTGGTTGAATTTTGTACGAGTTATTGATTGGTTTATTGGTGCTTTTCTAATTTACTTTTGAGAAGGCATTCATTTCACTGCATATTCAAGTTTAGGTGAAATTGAAATTTCAAGGTTAAAATTAACTTGCTGAATTAAGTTTAAAGGGTGGAAATGAATTTTATTTTATCTTGAATAATATCATCTAGCTACTTGATTTTTGAAAAGTTACAAGTCTGATGATTCGGCAGTTAGTGGTATTCATGTACTTATCCAACAATGACAGTTAAGAGTTAGTCATCCTTAATATAAAAACAAATATTGTTAGAGACCAATTCACATAGGTATAGGACCTATTCTTCTTGGTCAAGGCTCATCATAATGGATTGATAACCTCAACCTTGAAGAGTTGAACTTTCCTAGGTATTTTCTCCATCAAATAAAACCTACCTGGATTTGACATTTACATAACTACTGTAGGAGGCCTATTATCTTTTCGTTTTATTCATTTCAACAGTTGTTATTGCCTTCATCCTGATGGAATTAGCAACTGATGTTGAATTGTCTCAGTCTCTTCAATTTCTCTTCAATTTATTTTCTTGTAATAGATTTTTGTATGAGTATATTTGAAGAAGTGGATGGCCTCAGACAATGACTTTTGTTTCACTATTTTTAATTTTGTTTTCTTAATATTATGAGGATGAAATGGAACATTTTAAGCTTTGATGTTTTGTAGGATGCAGTACTATTTGAGAATGGGTAGGTTCTTTGCTTTTCTCAATAAGTCTTGCTTTGATCTCTATATGTAGTTTTTCTATGAATTCCTTATGGTGCAAAGGGAATACTAATGGGATCATTTACTTAATATTAATCTGCCTGATTATTCAAATATATAACAATTTCAAGTTAACAATTACTTTAGTAAGACAATTCAGAATAAAAAGTGAATTTAATAAGATGTGAAATCTGTGCCAAGCAGACTCTTGATAAAATTTTGTTAATTTCTAAGATATATCCTATTATGATTATAGGTTATAATAGATAGTTGTAGAACTAATTTCCTGCAAAGTGTGGGTGGAGGCTGGACCTATGACTCAAGATTTGCATAGTGCGAGTTTTCCCAACTGCGTTGATAACTTGGGTAAAAGCAACTGCTCCACAATCACTAAACCCGAGTAACAAAGTGCAAGTGTGCTAACACAGAATCATGCTTTGCTGAAGAGTGCAGGAGATTCTGAGGGAATTATACGCGCTTCCACTGAAAAATTGGCATTTAACTTTCTTTCTTTATGGTCTTCTTTCAGGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGTGTGCCTCCATTTTGGGCAGGTAAAAGAGAGAGTTACTTTCCTTTTCCCATTGTCATGACATATGCCTCCTTATATCGAAAATCTGTCACATCTTCATTTTCTGAAAATGCCTGGCCAGTGCTGTGAATAACATGACCTTTATTGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATCGACTTTGAATCTGATCCATGGCCAAAAATTTCTGCCAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAAGAAGGATTACGTCGGCGGAGGTTCTCGGTATGTCCTTTTCTTCTCTGTGAATGCTTTCTTGATACCTGAATGAAATCTGCCAAGTGGTATATGAAACTGTGGCATCTAACTGGGGATCGGACATTGTCTATCTTATTTAGAGCATCCATGGCTGAGAGAAGGTGGAGAGGCATCGGATAAGCCAATAGACAGTGCCGTATTTACAAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCTTGAAGGTGATCTTCCTTACACTTAGGCTTGGTTTTCTAGATAGAAGTAATATTTAATCAAGGGGCCGGGGATTAAGAGTTCTCGGTAATGGTAGTTGTTTGGGGGGGGGGGGGGGGGGATGAGGAGGGGACTGAAGAAGTTCAAGATATTAACAACTAGTTTATGAAACAATTGCAGGTTATAGCTGAAAATCTTTCGGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCATGAACATGGACACCGACAAGAGCGGAACCATCACTTATGCAGAACTGAAGTCTGGACTGGCTCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGTAATATATGATTTAAGATTATTGATCATCCAAAGTTTCTTATTTTCTTTTTCCTCACATGAACTGAGAGAACTTGATTTGCCGTCTCAGGCCGATGTGGATGGAAATGGTACAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTCTACAAAGCCTTTCTGCATTTTGACAAGGATAACAGTGGGTCAGTCTCTCATCCTAGAATATATAAGCATTTATCTGCTTTTTGAAACAACATATTCTAGTTTGATTGAGTATTTGCTTCTTGTTCTTTCAGTCATATCACAAGAGATGAACTAAAAGCTGCAATGAAAGAGTACGGGATGGGCGATGACGAGACCATTAGAGAAATCATTTCTGAAGTCGATGCAGACAATGTAAGTTTGTTCATATTTACAATTTGAAGTTCTAGCTAATCCTCTCTGGTAAAATGCAACTAATTTATGTTTGGGTTTCATAGGATGGAAGAATCAACTATGATGAGTTCTGTACAATGATGAGAAGTGGAACCCAACAAGCCAAACTCTTCTAACATGTAGATCGTCAAAGGGATCATCTACAGCGAGATCGCTGTCAAAAAGCTTGCCCGTCGACAGCAAAATGTCCAGACTACACCTAAAATGTTGGTTTTGGGTTTTAATGTGTGATATTTTTGCATAGCAATTGAATTGTTGTTTATATTTTTGTGTACAATTTATTGGGGTGTATAAGCCATGAATATGCTTAAGGATAGTTGTTTGATGGGAGAATTTGTATTGCCTAAATGTATTTGTTTCCTTGAGTATTAATTACGTCCACTTGATTTAGAATCTATTTTGGGTGAAACTGATGTTGACTTGGCCCAGCATTAACAGATTTGAGATTGGATTCCCCTGACCATTTCAAGCTGCTTATTTTTTAAATGTTGCCTTTGCTTGAATAAAAAATGGAAGCAGGAAAAGAGAGGAAGAAATGGACTTGGAAAGAGTCAAGGCTTAAAATGGTTGTATATTTTGTTTTGGTTTTTTAAAAATTGGGTTTTTTTTTTGATGGTTAACGTACCATTTGAGTTTGGCATTTTTAACGTTGGTGACGTTTAGGGGGCATTTGGTGCTCTGGTTCGAAATTAGAAACCCTATGAATCGGATTACGATCAGATTCATTGTCTGTAATCTGAAACCCTAAGGTTGTTGAAGTCAGTGTGTTGTGTTGTGTTGGTTTTTTTGTTTAGCTTGTTTTTAGTTGATTTTTTTTCTTCCGAATCTATATTTTTTTACTCAGTTCATTAGGCTTGTTCACATAACATTAACAATGTCTAAC

mRNA sequence

ATGGGCATCTGCACAAGCAAAGGAAAATACTCACCTCAGTATGATCGTTATGAACAGCAAGCAAAAGTCCCAGCTCAACACGACCACCGGTCGCCGGAAAATCACCATTCGGCGGCGGCGAAGTCAACGGGTGCTCCCAAAAGCCCCAGACACAACATTCTCAGGTCTGATACGATTCTTGGGAAGCCATTAGAGGATGTTAAGCTGCATTACACGATTGGGAAAGAATTGGGGAGAGGGCAATTTGGGGTTACTTATCTGTGCACTGAGAATTCCTCAGGTAAGCAATATGCTTGCAAGTCCATTTCGAAGAGGAAGCTTGTGACCAGAACTGATAAGGAGGATATCAGGAGGGAGATTCACATCATGCAGCATTTGAGTGGGCAGCCCAACATAGTTGAGTTCAAGGGAGCTTATGAAGACAAGCAATCGGTTCATTTGATCATGGAGCTTTGCGCTGGAGGCGAGCTGTTTGATAGAATTATTGCAAAGGGTCATTACAGTGAGAAAGCAGCTGCCTCAATCTGCAGGTCAATTGTTAATGTGGTTCATATCTGCCATTTCATGGGGGTGATGCACAGGGACCTCAAGCCTGAGAACTTTCTGCTTGCTAGCAAAGACGAAGATGCTATGCTCAAAGCAACAGATTTTGGATTGTCTGTTTTTATTGAAGATGGTGAGGTTCTTGCTCTGCTTATTGGATTTGTTCTTCATGCCTTTTTGATTGAGTGTCTTTCTGTGCTTGTTTTAGTTGACTTTGCTGTTCTTGTTAATGGTTTTAGAAGGATGCAGTACTATTTGAGAATGGGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGTGTGCCTCCATTTTGGGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATCGACTTTGAATCTGATCCATGGCCAAAAATTTCTGCCAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAAGAAGGATTACGTCGGCGGAGGTTCTCGAGCATCCATGGCTGAGAGAAGGTGGAGAGGCATCGGATAAGCCAATAGACAGTGCCGTATTTACAAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCTTGAAGGTTATAGCTGAAAATCTTTCGGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCATGAACATGGACACCGACAAGAGCGGAACCATCACTTATGCAGAACTGAAGTCTGGACTGGCTCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGCCGATGTGGATGGAAATGGTACAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTCTACAAAGCCTTTCTGCATTTTGACAAGGATAACAGTGGTCATATCACAAGAGATGAACTAAAAGCTGCAATGAAAGAGTACGGGATGGGCGATGACGAGACCATTAGAGAAATCATTTCTGAAGTCGATGCAGACAATGATGGAAGAATCAACTATGATGAGTTCTGTACAATGATGAGAAGTGGAACCCAACAAGCCAAACTCTTCTAA

Coding sequence (CDS)

ATGGGCATCTGCACAAGCAAAGGAAAATACTCACCTCAGTATGATCGTTATGAACAGCAAGCAAAAGTCCCAGCTCAACACGACCACCGGTCGCCGGAAAATCACCATTCGGCGGCGGCGAAGTCAACGGGTGCTCCCAAAAGCCCCAGACACAACATTCTCAGGTCTGATACGATTCTTGGGAAGCCATTAGAGGATGTTAAGCTGCATTACACGATTGGGAAAGAATTGGGGAGAGGGCAATTTGGGGTTACTTATCTGTGCACTGAGAATTCCTCAGGTAAGCAATATGCTTGCAAGTCCATTTCGAAGAGGAAGCTTGTGACCAGAACTGATAAGGAGGATATCAGGAGGGAGATTCACATCATGCAGCATTTGAGTGGGCAGCCCAACATAGTTGAGTTCAAGGGAGCTTATGAAGACAAGCAATCGGTTCATTTGATCATGGAGCTTTGCGCTGGAGGCGAGCTGTTTGATAGAATTATTGCAAAGGGTCATTACAGTGAGAAAGCAGCTGCCTCAATCTGCAGGTCAATTGTTAATGTGGTTCATATCTGCCATTTCATGGGGGTGATGCACAGGGACCTCAAGCCTGAGAACTTTCTGCTTGCTAGCAAAGACGAAGATGCTATGCTCAAAGCAACAGATTTTGGATTGTCTGTTTTTATTGAAGATGGTGAGGTTCTTGCTCTGCTTATTGGATTTGTTCTTCATGCCTTTTTGATTGAGTGTCTTTCTGTGCTTGTTTTAGTTGACTTTGCTGTTCTTGTTAATGGTTTTAGAAGGATGCAGTACTATTTGAGAATGGGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGTGTGCCTCCATTTTGGGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATCGACTTTGAATCTGATCCATGGCCAAAAATTTCTGCCAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAAGAAGGATTACGTCGGCGGAGGTTCTCGAGCATCCATGGCTGAGAGAAGGTGGAGAGGCATCGGATAAGCCAATAGACAGTGCCGTATTTACAAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCTTGAAGGTTATAGCTGAAAATCTTTCGGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCATGAACATGGACACCGACAAGAGCGGAACCATCACTTATGCAGAACTGAAGTCTGGACTGGCTCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGCCGATGTGGATGGAAATGGTACAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTCTACAAAGCCTTTCTGCATTTTGACAAGGATAACAGTGGTCATATCACAAGAGATGAACTAAAAGCTGCAATGAAAGAGTACGGGATGGGCGATGACGAGACCATTAGAGAAATCATTTCTGAAGTCGATGCAGACAATGATGGAAGAATCAACTATGATGAGTTCTGTACAATGATGAGAAGTGGAACCCAACAAGCCAAACTCTTCTAA

Protein sequence

MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINYDEFCTMMRSGTQQAKLF
Homology
BLAST of Spg000138 vs. NCBI nr
Match: XP_038903170.1 (calcium-dependent protein kinase 2-like [Benincasa hispida])

HSP 1 Score: 965.7 bits (2495), Expect = 1.7e-277
Identity = 492/567 (86.77%), Postives = 505/567 (89.07%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAA-----AKSTGAPKSPRHNILR 60
           MGICTSKGKYSPQYDRYEQQ K PAQH HRSPENHHSAA     AKS+G PKSP+H   R
Sbjct: 1   MGICTSKGKYSPQYDRYEQQPKAPAQHAHRSPENHHSAAAAAAKAKSSGPPKSPKHAAFR 60

Query: 61  SDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKED 120
           SDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTR DKED
Sbjct: 61  SDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRNDKED 120

Query: 121 IRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASI 180
           I+REIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASI
Sbjct: 121 IKREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASI 180

Query: 181 CRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGF 240
           CRSIVNVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+          
Sbjct: 181 CRSIVNVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE---------- 240

Query: 241 VLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKE 300
                                             GKVYRDIVGSAYYVAPEVLRRKYGKE
Sbjct: 241 ----------------------------------GKVYRDIVGSAYYVAPEVLRRKYGKE 300

Query: 301 IDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLT 360
           IDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWP+IS SAKDLVKKMLT
Sbjct: 301 IDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPQISKSAKDLVKKMLT 360

Query: 361 QDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSE 420
           QDPA RITSA+VLEHPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSE
Sbjct: 361 QDPANRITSAQVLEHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSE 420

Query: 421 EEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYI 480
           EEIQGLKAMF NMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYI
Sbjct: 421 EEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYI 480

Query: 481 EFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEV 540
           EFITATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEV
Sbjct: 481 EFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEV 523

Query: 541 DADNDGRINYDEFCTMMRSGTQQAKLF 563
           DADNDGRINYDEFC+MMRSGTQQAK F
Sbjct: 541 DADNDGRINYDEFCSMMRSGTQQAKCF 523

BLAST of Spg000138 vs. NCBI nr
Match: XP_023520424.1 (calcium-dependent protein kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957.6 bits (2474), Expect = 4.8e-275
Identity = 481/562 (85.59%), Postives = 502/562 (89.32%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTIL 60
           MG+C+SKGKY PQYDRYE Q K PAQH H+SPE+HHSAA KSTG P+SPRH +LRSD+IL
Sbjct: 1   MGVCSSKGKYKPQYDRYEHQPKGPAQHSHQSPESHHSAAVKSTGPPRSPRHTVLRSDSIL 60

Query: 61  GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREI 120
           GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTR DK+DIRREI
Sbjct: 61  GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRNDKDDIRREI 120

Query: 121 HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV 180
           HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV
Sbjct: 121 HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV 180

Query: 181 NVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAF 240
           NVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+               
Sbjct: 181 NVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE--------------- 240

Query: 241 LIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS 300
                                        GKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS
Sbjct: 241 -----------------------------GKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS 300

Query: 301 AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPAR 360
           AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFE+DPWPKIS SAKDLVKKMLTQDPA+
Sbjct: 301 AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFETDPWPKISNSAKDLVKKMLTQDPAQ 360

Query: 361 RITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG 420
           RITSA+VL+HPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG
Sbjct: 361 RITSAQVLDHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG 420

Query: 421 LKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITA 480
           LK  F NMDTD SGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNG+IDYIEFITA
Sbjct: 421 LKTTFTNMDTDNSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGSIDYIEFITA 480

Query: 481 TMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADND 540
           TMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEVDADND
Sbjct: 481 TMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADND 518

Query: 541 GRINYDEFCTMMRSGTQQAKLF 563
           GRINYDEFCTMMR+GTQQ KLF
Sbjct: 541 GRINYDEFCTMMRTGTQQGKLF 518

BLAST of Spg000138 vs. NCBI nr
Match: XP_008463413.1 (PREDICTED: calcium-dependent protein kinase-like [Cucumis melo] >KAA0033976.1 calcium-dependent protein kinase-like [Cucumis melo var. makuwa] >TYK02570.1 calcium-dependent protein kinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 954.1 bits (2465), Expect = 5.3e-274
Identity = 487/563 (86.50%), Postives = 502/563 (89.17%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH-SAAAKSTGAPKSPRHNILRSDTI 60
           MGICTSKGKYS QYDRYEQQ K PAQH HRSPE H  +AAAKS+G PKSP+H   RSDTI
Sbjct: 1   MGICTSKGKYSRQYDRYEQQPKAPAQHAHRSPEYHQPAAAAKSSGPPKSPKHTPFRSDTI 60

Query: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRRE 120
           LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSI KRKLVTR DKEDIRRE
Sbjct: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSILKRKLVTRNDKEDIRRE 120

Query: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180
           IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI
Sbjct: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180

Query: 181 VNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHA 240
           VNVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+              
Sbjct: 181 VNVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE-------------- 240

Query: 241 FLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300
                                         GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW
Sbjct: 241 ------------------------------GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300

Query: 301 SAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPA 360
           SAGVMLYILLSGVPPFWAETEKGIFD+ILQGHIDFES+PWP+IS SAKDLVKKMLTQDPA
Sbjct: 301 SAGVMLYILLSGVPPFWAETEKGIFDSILQGHIDFESEPWPQISHSAKDLVKKMLTQDPA 360

Query: 361 RRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420
            RITSA+VLEHPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ
Sbjct: 361 NRITSAQVLEHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420

Query: 421 GLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480
           GLKAMF NMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT
Sbjct: 421 GLKAMFTNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480

Query: 481 ATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADN 540
           ATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEVDADN
Sbjct: 481 ATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADN 519

Query: 541 DGRINYDEFCTMMRSGTQQAKLF 563
           DGRINYDEFC+MMRSGTQQAKLF
Sbjct: 541 DGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Spg000138 vs. NCBI nr
Match: KAG6600782.1 (Calcium-dependent protein kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 953.4 bits (2463), Expect = 9.0e-274
Identity = 483/564 (85.64%), Postives = 503/564 (89.18%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH--SAAAKSTGAPKSPRHNILRSDT 60
           MGICTSKGKYSPQYDRYE QAK PAQH HRSPEN+H  + AAKS+G PKSPRHNILRSDT
Sbjct: 1   MGICTSKGKYSPQYDRYENQAKPPAQHAHRSPENYHPEATAAKSSGPPKSPRHNILRSDT 60

Query: 61  ILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRR 120
           ILGKP EDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVT+ DK+DIRR
Sbjct: 61  ILGKPFEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTKNDKDDIRR 120

Query: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180
           EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS
Sbjct: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180

Query: 181 IVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLH 240
           IVNVVHICHFMGVMHRDLKPENFLLASK+E+AMLKATDFGLSVFIE+             
Sbjct: 181 IVNVVHICHFMGVMHRDLKPENFLLASKEENAMLKATDFGLSVFIEE------------- 240

Query: 241 AFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDI 300
                                          GKVY+DIVGSAYYVAPEVLRR+YGKEIDI
Sbjct: 241 -------------------------------GKVYKDIVGSAYYVAPEVLRRRYGKEIDI 300

Query: 301 WSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDP 360
           WSAGVMLYILLSGVPPFWAETEKGIFD+ILQGH+DFE+DPWPKIS SAKDLVKKML  D 
Sbjct: 301 WSAGVMLYILLSGVPPFWAETEKGIFDSILQGHLDFETDPWPKISNSAKDLVKKMLNPDA 360

Query: 361 ARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420
           ++R+TSA+VLEHPWLREGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI
Sbjct: 361 SKRLTSAQVLEHPWLREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420

Query: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480
           QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI
Sbjct: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480

Query: 481 TATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDAD 540
           TATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD ETI EIISEVDAD
Sbjct: 481 TATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDKETINEIISEVDAD 520

Query: 541 NDGRINYDEFCTMMRSGTQQAKLF 563
           NDGRINYDEFCTMMRSGTQQ KLF
Sbjct: 541 NDGRINYDEFCTMMRSGTQQVKLF 520

BLAST of Spg000138 vs. NCBI nr
Match: KAG7031419.1 (Calcium-dependent protein kinase 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 482/564 (85.46%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH--SAAAKSTGAPKSPRHNILRSDT 60
           MGICTSKGKYSPQYDRYE QAK+PAQH HRSPEN+H  + AAKS+G PKSPRHNILRSDT
Sbjct: 1   MGICTSKGKYSPQYDRYENQAKLPAQHAHRSPENYHPEATAAKSSGPPKSPRHNILRSDT 60

Query: 61  ILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRR 120
           ILGKP EDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVT+ DK+DIRR
Sbjct: 61  ILGKPFEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTKNDKDDIRR 120

Query: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180
           EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIA+GHYSEKAAASICRS
Sbjct: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAEGHYSEKAAASICRS 180

Query: 181 IVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLH 240
           IVNVVHICHFMGVMHRDLKPENFLLASK+E+AMLKATDFGLSVFIE+             
Sbjct: 181 IVNVVHICHFMGVMHRDLKPENFLLASKEENAMLKATDFGLSVFIEE------------- 240

Query: 241 AFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDI 300
                                          GKVY+DIVGSAYYVAPEVLRR+YGKEIDI
Sbjct: 241 -------------------------------GKVYKDIVGSAYYVAPEVLRRRYGKEIDI 300

Query: 301 WSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDP 360
           WSAGVMLYILLSGVPPFWAETEKGIFD+ILQGH+DFE+DPWPKIS SAKDLVKKML  D 
Sbjct: 301 WSAGVMLYILLSGVPPFWAETEKGIFDSILQGHLDFETDPWPKISNSAKDLVKKMLNPDA 360

Query: 361 ARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420
           ++R+TSA+VLEHPWLREGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI
Sbjct: 361 SKRLTSAQVLEHPWLREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420

Query: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480
           QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI
Sbjct: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480

Query: 481 TATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDAD 540
           TATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD ETI EIISEVDAD
Sbjct: 481 TATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDKETINEIISEVDAD 520

Query: 541 NDGRINYDEFCTMMRSGTQQAKLF 563
           NDGRINYDEFCTMMRSGTQQ KLF
Sbjct: 541 NDGRINYDEFCTMMRSGTQQVKLF 520

BLAST of Spg000138 vs. ExPASy Swiss-Prot
Match: Q38868 (Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.3e-219
Identity = 389/556 (69.96%), Postives = 454/556 (81.65%), Query Frame = 0

Query: 10  YSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTILGKPLEDVKL 69
           Y+PQ  R  QQ + P   + + P    +AAA    +PK+      +S++IL    EDVKL
Sbjct: 36  YTPQ-ARTTQQPEKPGSVNSQPPPWRAAAAAPGL-SPKT----TTKSNSILENAFEDVKL 95

Query: 70  HYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQHLSGQ 129
            YT+GKELGRGQFGVTYLCTENS+GK+YACKSISK+KLVT+ DK+D+RREI IMQHLSGQ
Sbjct: 96  FYTLGKELGRGQFGVTYLCTENSTGKKYACKSISKKKLVTKADKDDMRREIQIMQHLSGQ 155

Query: 130 PNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHICHFM 189
           PNIVEFKGAYED+++V+L+MELCAGGELFDRIIAKGHY+E+AAAS+CR IVNVV ICHFM
Sbjct: 156 PNIVEFKGAYEDEKAVNLVMELCAGGELFDRIIAKGHYTERAAASVCRQIVNVVKICHFM 215

Query: 190 GVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECLSVLV 249
           GV+HRDLKPENFLL+SKDE A++KATDFGLSVFIE+                        
Sbjct: 216 GVLHRDLKPENFLLSSKDEKALIKATDFGLSVFIEE------------------------ 275

Query: 250 LVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILL 309
                               GKVYRDIVGSAYYVAPEVLRR+YGKE+DIWSAG++LYILL
Sbjct: 276 --------------------GKVYRDIVGSAYYVAPEVLRRRYGKEVDIWSAGIILYILL 335

Query: 310 SGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSAEVLE 369
           SGVPPFWAETEKGIFDAIL+GHIDFES PWP IS+SAKDLV++MLT DP RRI++A+VL+
Sbjct: 336 SGVPPFWAETEKGIFDAILEGHIDFESQPWPSISSSAKDLVRRMLTADPKRRISAADVLQ 395

Query: 370 HPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFMNMD 429
           HPWLREGGEASDKPIDSAV +R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+D
Sbjct: 396 HPWLREGGEASDKPIDSAVLSRMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANID 455

Query: 430 TDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEK 489
           TD SGTITY ELK GLA+LGS L+EAEVKQLM+AADVDGNG+IDYIEFITATMHR++LE 
Sbjct: 456 TDNSGTITYEELKEGLAKLGSKLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLES 515

Query: 490 EEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINYDEFC 549
            E+LYKAF HFDKD+SG+IT DEL++A+KEYGMGDD TI+E++S+VD+DNDGRINY+EFC
Sbjct: 516 NENLYKAFQHFDKDSSGYITIDELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEEFC 541

Query: 550 TMMRSGT---QQAKLF 563
            MMRSG    QQ +LF
Sbjct: 576 AMMRSGNPQQQQPRLF 541

BLAST of Spg000138 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-218
Identity = 392/569 (68.89%), Postives = 455/569 (79.96%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSP------RHNIL 60
           MG C +K KY     + +Q  +   + ++R    H   +  STG  + P      +H+  
Sbjct: 1   MGNCLAK-KYG-LVMKPQQNGERSVEIENRRRSTHQDPSKISTGTNQPPPWRNPAKHS-- 60

Query: 61  RSDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKE 120
            +  IL KP EDVKL YT+ KELGRGQFGVTYLCTE S+GK++ACKSISK+KLVT+ DKE
Sbjct: 61  GAAAILEKPYEDVKLFYTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKE 120

Query: 121 DIRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAAS 180
           D+RREI IMQHLSGQPNIVEFKGAYED+++V+L+MELCAGGELFDRI+AKGHYSE+AAAS
Sbjct: 121 DMRREIQIMQHLSGQPNIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAAS 180

Query: 181 ICRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIG 240
           +CR IVNVV+ICHFMGVMHRDLKPENFLL+SKDE A++KATDFGLSVFIE+         
Sbjct: 181 VCRQIVNVVNICHFMGVMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEE--------- 240

Query: 241 FVLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGK 300
                                              G+VY+DIVGSAYYVAPEVL+R+YGK
Sbjct: 241 -----------------------------------GRVYKDIVGSAYYVAPEVLKRRYGK 300

Query: 301 EIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKML 360
           EIDIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G IDFES PWP IS SAKDLV++ML
Sbjct: 301 EIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRML 360

Query: 361 TQDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLS 420
           TQDP RRI++AEVL+HPWLREGGEASDKPIDSAV +R+KQFRAMNKLKKLALKVIAEN+ 
Sbjct: 361 TQDPKRRISAAEVLKHPWLREGGEASDKPIDSAVLSRMKQFRAMNKLKKLALKVIAENID 420

Query: 421 EEEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDY 480
            EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS L+EAEVKQLM+AADVDGNG+IDY
Sbjct: 421 TEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGSRLTEAEVKQLMDAADVDGNGSIDY 480

Query: 481 IEFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISE 540
           IEFITATMHR++LE  E++YKAF HFDKD SG+IT DEL+AA+KEYGMGDD TI+EI+S+
Sbjct: 481 IEFITATMHRHRLESNENVYKAFQHFDKDGSGYITTDELEAALKEYGMGDDATIKEILSD 521

Query: 541 VDADNDGRINYDEFCTMMRSGT-QQAKLF 563
           VDADNDGRINYDEFC MMRSG  QQ +LF
Sbjct: 541 VDADNDGRINYDEFCAMMRSGNPQQPRLF 521

BLAST of Spg000138 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 758.4 bits (1957), Expect = 5.6e-218
Identity = 391/572 (68.36%), Postives = 444/572 (77.62%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQ---------QAKVPAQHDHRSPENHHSAAAKSTGAPKSPRH 60
           MG C SK +Y    + Y                       P+       + TG    PR 
Sbjct: 1   MGGCFSKKEYQADGNGYRSAPTAYHTVTDQSYEKSSQRSQPQAQPQPQVQQTGPSLKPRQ 60

Query: 61  -NILRSDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTR 120
            +   S+TILGKP ED++  YT+GKELGRGQFG  Y CTENSSG+ YACKSI KRKLV++
Sbjct: 61  VHRPESNTILGKPFEDIRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSK 120

Query: 121 TDKEDIRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEK 180
            DKEDI+REI I+QHLSGQPNIVEFKG +ED+QSVHL+MELCAGGELFDRIIA+GHYSE+
Sbjct: 121 NDKEDIKREIQILQHLSGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSER 180

Query: 181 AAASICRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLA 240
           AAA+ICR IVNVVH+CHFMGVMHRDLKPENFLL+SKD+DAMLKATDFGLSVFIE+     
Sbjct: 181 AAATICRQIVNVVHVCHFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEE----- 240

Query: 241 LLIGFVLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRR 300
                                                  GKVYR+IVGSAYYVAPEVLRR
Sbjct: 241 ---------------------------------------GKVYRNIVGSAYYVAPEVLRR 300

Query: 301 KYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLV 360
            YGKEIDIWSAGV+LYILLSGVPPFWAE EKGIFDAIL+G IDFES+PWP +S SAKDLV
Sbjct: 301 SYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDLV 360

Query: 361 KKMLTQDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIA 420
           +KMLTQDP RRITSA+VL+HPW+REGGEASDKPIDSAV +R+KQFRAMNKLK+LALKVIA
Sbjct: 361 RKMLTQDPRRRITSAQVLDHPWMREGGEASDKPIDSAVLSRMKQFRAMNKLKQLALKVIA 420

Query: 421 ENLSEEEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNG 480
           E+LSEEEI+GLK+MF NMDTDKSGTITY ELKSGLARLGS LSE EV+QLM+AADVDGNG
Sbjct: 421 ESLSEEEIKGLKSMFANMDTDKSGTITYEELKSGLARLGSKLSEVEVQQLMDAADVDGNG 480

Query: 481 TIDYIEFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIRE 540
           TIDY+EFITATMHR+KLE  EH  +AF +FDKDNSG IT+DEL++AMKEYGMGD+ TI++
Sbjct: 481 TIDYLEFITATMHRHKLESYEH--QAFQYFDKDNSGFITKDELESAMKEYGMGDEATIKD 526

Query: 541 IISEVDADNDGRINYDEFCTMMRSGTQQAKLF 563
           IISEVD+DNDGRINYDEFC MMR    + KLF
Sbjct: 541 IISEVDSDNDGRINYDEFCAMMRRARNRRKLF 526

BLAST of Spg000138 vs. ExPASy Swiss-Prot
Match: P49101 (Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 1.5e-215
Identity = 384/553 (69.44%), Postives = 442/553 (79.93%), Query Frame = 0

Query: 6   SKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTILGKPLE 65
           S+ K+   Y+  +Q A+V       +P   +S+A      P  P      +DTILGK  E
Sbjct: 10  SRRKHLRVYNPPQQAAEV-----RYTPSATNSSAVPPVAVPPKP-----TADTILGKQYE 69

Query: 66  DVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQH 125
           DV+  Y+ GKELGRGQFGVTYLCTE +SG+QYACKSISKRKLV++ D+EDIRREI IMQH
Sbjct: 70  DVRSVYSFGKELGRGQFGVTYLCTEIASGRQYACKSISKRKLVSKADREDIRREIQIMQH 129

Query: 126 LSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHI 185
           LSGQPNIVEF+GAYEDK +VH++MELCAGGELFDRIIAKGHY+E+AAA+ICR++VNVV+I
Sbjct: 130 LSGQPNIVEFRGAYEDKSNVHVVMELCAGGELFDRIIAKGHYTERAAATICRAVVNVVNI 189

Query: 186 CHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECL 245
           CHFMGVMHRDLKPENFLLA+ +E+AMLKATDFGLSVFIE+                    
Sbjct: 190 CHFMGVMHRDLKPENFLLATMEENAMLKATDFGLSVFIEE-------------------- 249

Query: 246 SVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVML 305
                                   GK+YRDIVGSAYYVAPEVLRR YGKEID+WSAGV+L
Sbjct: 250 ------------------------GKMYRDIVGSAYYVAPEVLRRSYGKEIDVWSAGVIL 309

Query: 306 YILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSA 365
           YILLSGVPPFWAE EKGIFDAIL   IDFES PWP IS SAKDLV+KMLT+DP +R+TSA
Sbjct: 310 YILLSGVPPFWAEIEKGIFDAILHEEIDFESQPWPSISESAKDLVRKMLTRDPKKRLTSA 369

Query: 366 EVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMF 425
           +VL+H WLREGGEASDKPIDSAV +R+KQFRAMNKLKK+ALKVIA NL+EEEI+GLK MF
Sbjct: 370 QVLQHQWLREGGEASDKPIDSAVLSRMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQMF 429

Query: 426 MNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRY 485
           MNMDTD SGTITY ELK+GLA+LGS LSEAEVKQLMEAADVDGNG+IDY+EFITATMHR+
Sbjct: 430 MNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITATMHRH 489

Query: 486 KLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINY 545
           KLE++EHL+KAF +FDKDNSG ITRDEL++A+ E+ MGD  TIREIISEVD DNDGRINY
Sbjct: 490 KLERDEHLFKAFQYFDKDNSGFITRDELESALIEHEMGDTSTIREIISEVDTDNDGRINY 508

Query: 546 DEFCTMMRSGTQQ 559
           +EFC MMR G QQ
Sbjct: 550 EEFCAMMRGGMQQ 508

BLAST of Spg000138 vs. ExPASy Swiss-Prot
Match: P53683 (Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK19 PE=2 SV=2)

HSP 1 Score: 748.0 bits (1930), Expect = 7.5e-215
Identity = 386/554 (69.68%), Postives = 442/554 (79.78%), Query Frame = 0

Query: 5   TSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTILGKPL 64
           T   + +P Y+    Q   PA+  + +P   +        AP  P       DTILGKP 
Sbjct: 27  TKPAQTTPSYN--HPQPPPPAEVRY-TPSAMNPPVVPPVVAPPKP-----TPDTILGKPY 86

Query: 65  EDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQ 124
           +DV+  Y++GKELGRGQFGVTYLCTE +SGKQYACKSISKRKLV++ DKEDIRREI IMQ
Sbjct: 87  DDVRSVYSLGKELGRGQFGVTYLCTEIASGKQYACKSISKRKLVSKADKEDIRREIQIMQ 146

Query: 125 HLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVH 184
           HLSGQ NIVEF+GAYEDK +VH++MELCAGGELFDRIIAKGHYSE+AAA+ICR++VNVV+
Sbjct: 147 HLSGQQNIVEFRGAYEDKSNVHVVMELCAGGELFDRIIAKGHYSERAAATICRAVVNVVN 206

Query: 185 ICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIEC 244
           ICHFMGVMHRDLKPENFLLA+K+E+AMLKATDFGLSVFIE+                   
Sbjct: 207 ICHFMGVMHRDLKPENFLLATKEENAMLKATDFGLSVFIEE------------------- 266

Query: 245 LSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVM 304
                                    GK+YRDIVGSAYYVAPEVLRR YGKEID+WSAGV+
Sbjct: 267 -------------------------GKMYRDIVGSAYYVAPEVLRRNYGKEIDVWSAGVI 326

Query: 305 LYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITS 364
           LYILLSGVPPFWAETEKGIFDAILQG IDFES PWP IS SAKDLV+KMLTQDP +RITS
Sbjct: 327 LYILLSGVPPFWAETEKGIFDAILQGEIDFESQPWPSISESAKDLVRKMLTQDPKKRITS 386

Query: 365 AEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAM 424
           A+VL+HPWLR+ GEASDKPIDSAV +R+KQFRAMNKLKK+ALKVIA NL+EEEI+GLK M
Sbjct: 387 AQVLQHPWLRD-GEASDKPIDSAVLSRMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQM 446

Query: 425 FMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHR 484
           F NMDTD SGTITY ELK+GLA+LGS LSEAEVKQLMEAADVDGNG+IDY+EFITATMHR
Sbjct: 447 FTNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDYVEFITATMHR 506

Query: 485 YKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRIN 544
           +KLE++EHL+KAF +FDKDNSG ITRDEL++A+ E+ MGD  TI++IISEVD DNDGRIN
Sbjct: 507 HKLERDEHLFKAFQYFDKDNSGFITRDELESALIEHEMGDTSTIKDIISEVDTDNDGRIN 527

Query: 545 YDEFCTMMRSGTQQ 559
           Y+EFC MMR G  Q
Sbjct: 567 YEEFCAMMRGGGMQ 527

BLAST of Spg000138 vs. ExPASy TrEMBL
Match: A0A5A7SVI6 (Calcium-dependent protein kinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00220 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.5e-274
Identity = 487/563 (86.50%), Postives = 502/563 (89.17%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH-SAAAKSTGAPKSPRHNILRSDTI 60
           MGICTSKGKYS QYDRYEQQ K PAQH HRSPE H  +AAAKS+G PKSP+H   RSDTI
Sbjct: 1   MGICTSKGKYSRQYDRYEQQPKAPAQHAHRSPEYHQPAAAAKSSGPPKSPKHTPFRSDTI 60

Query: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRRE 120
           LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSI KRKLVTR DKEDIRRE
Sbjct: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSILKRKLVTRNDKEDIRRE 120

Query: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180
           IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI
Sbjct: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180

Query: 181 VNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHA 240
           VNVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+              
Sbjct: 181 VNVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE-------------- 240

Query: 241 FLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300
                                         GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW
Sbjct: 241 ------------------------------GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300

Query: 301 SAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPA 360
           SAGVMLYILLSGVPPFWAETEKGIFD+ILQGHIDFES+PWP+IS SAKDLVKKMLTQDPA
Sbjct: 301 SAGVMLYILLSGVPPFWAETEKGIFDSILQGHIDFESEPWPQISHSAKDLVKKMLTQDPA 360

Query: 361 RRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420
            RITSA+VLEHPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ
Sbjct: 361 NRITSAQVLEHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420

Query: 421 GLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480
           GLKAMF NMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT
Sbjct: 421 GLKAMFTNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480

Query: 481 ATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADN 540
           ATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEVDADN
Sbjct: 481 ATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADN 519

Query: 541 DGRINYDEFCTMMRSGTQQAKLF 563
           DGRINYDEFC+MMRSGTQQAKLF
Sbjct: 541 DGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Spg000138 vs. ExPASy TrEMBL
Match: A0A1S3CJN0 (calcium-dependent protein kinase-like OS=Cucumis melo OX=3656 GN=LOC103501584 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.5e-274
Identity = 487/563 (86.50%), Postives = 502/563 (89.17%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH-SAAAKSTGAPKSPRHNILRSDTI 60
           MGICTSKGKYS QYDRYEQQ K PAQH HRSPE H  +AAAKS+G PKSP+H   RSDTI
Sbjct: 1   MGICTSKGKYSRQYDRYEQQPKAPAQHAHRSPEYHQPAAAAKSSGPPKSPKHTPFRSDTI 60

Query: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRRE 120
           LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSI KRKLVTR DKEDIRRE
Sbjct: 61  LGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSILKRKLVTRNDKEDIRRE 120

Query: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180
           IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI
Sbjct: 121 IHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSI 180

Query: 181 VNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHA 240
           VNVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+              
Sbjct: 181 VNVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE-------------- 240

Query: 241 FLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300
                                         GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW
Sbjct: 241 ------------------------------GKVYRDIVGSAYYVAPEVLRRKYGKEIDIW 300

Query: 301 SAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPA 360
           SAGVMLYILLSGVPPFWAETEKGIFD+ILQGHIDFES+PWP+IS SAKDLVKKMLTQDPA
Sbjct: 301 SAGVMLYILLSGVPPFWAETEKGIFDSILQGHIDFESEPWPQISHSAKDLVKKMLTQDPA 360

Query: 361 RRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420
            RITSA+VLEHPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ
Sbjct: 361 NRITSAQVLEHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQ 420

Query: 421 GLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480
           GLKAMF NMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT
Sbjct: 421 GLKAMFTNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFIT 480

Query: 481 ATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADN 540
           ATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEVDADN
Sbjct: 481 ATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADN 519

Query: 541 DGRINYDEFCTMMRSGTQQAKLF 563
           DGRINYDEFC+MMRSGTQQAKLF
Sbjct: 541 DGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Spg000138 vs. ExPASy TrEMBL
Match: A0A6J1IGJ9 (calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111476745 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.3e-273
Identity = 482/564 (85.46%), Postives = 503/564 (89.18%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH--SAAAKSTGAPKSPRHNILRSDT 60
           MGICTSKGKYSPQYDRYE QAK PAQH HRSP N+H  + AAKS+G PKSPRHNILRSDT
Sbjct: 1   MGICTSKGKYSPQYDRYENQAKPPAQHAHRSPGNYHPEATAAKSSGPPKSPRHNILRSDT 60

Query: 61  ILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRR 120
           ILGKP EDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVT+ DK+DIRR
Sbjct: 61  ILGKPFEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTKNDKDDIRR 120

Query: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180
           EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS
Sbjct: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180

Query: 181 IVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLH 240
           IVNVVHICHFMGVMHRDLKPENFLLASK+E+AMLKATDFGLSVFIE+             
Sbjct: 181 IVNVVHICHFMGVMHRDLKPENFLLASKEENAMLKATDFGLSVFIEE------------- 240

Query: 241 AFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDI 300
                                          GKVY+DIVGSAYYVAPEVLRR+YGKEIDI
Sbjct: 241 -------------------------------GKVYKDIVGSAYYVAPEVLRRRYGKEIDI 300

Query: 301 WSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDP 360
           WSAGVMLYILLSGVPPFWAETEKGIFD+ILQGH+DFE+DPWPKIS SAKDLVKKML  D 
Sbjct: 301 WSAGVMLYILLSGVPPFWAETEKGIFDSILQGHLDFETDPWPKISNSAKDLVKKMLNPDA 360

Query: 361 ARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420
           ++R+TSA+VLEHPWLREGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI
Sbjct: 361 SKRLTSAQVLEHPWLREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420

Query: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480
           QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI
Sbjct: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480

Query: 481 TATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDAD 540
           TATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETI EIISEVDAD
Sbjct: 481 TATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETINEIISEVDAD 520

Query: 541 NDGRINYDEFCTMMRSGTQQAKLF 563
           NDGRINYDEFCTMMRSGTQQ KLF
Sbjct: 541 NDGRINYDEFCTMMRSGTQQVKLF 520

BLAST of Spg000138 vs. ExPASy TrEMBL
Match: A0A6J1F0W0 (calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111438480 PE=4 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 479/562 (85.23%), Postives = 501/562 (89.15%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTIL 60
           MG+C+SKGKY PQYDRYE Q K PAQH H+SPE+HHSAAAKSTG P+SP   +LRSD+IL
Sbjct: 1   MGVCSSKGKYKPQYDRYEHQPKGPAQHSHQSPESHHSAAAKSTGPPRSPLPTVLRSDSIL 60

Query: 61  GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREI 120
           GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTR DK+DIRREI
Sbjct: 61  GKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRNDKDDIRREI 120

Query: 121 HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV 180
           HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV
Sbjct: 121 HIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIV 180

Query: 181 NVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAF 240
           NVVHICHFMGVMHRDLKPENFLLASK+EDAMLKATDFGLSVFIE+               
Sbjct: 181 NVVHICHFMGVMHRDLKPENFLLASKEEDAMLKATDFGLSVFIEE--------------- 240

Query: 241 LIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS 300
                                        GKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS
Sbjct: 241 -----------------------------GKVYRDIVGSAYYVAPEVLRRKYGKEIDIWS 300

Query: 301 AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPAR 360
           AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFE+DPWPKIS SAKDLVKKMLTQDPA+
Sbjct: 301 AGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFETDPWPKISNSAKDLVKKMLTQDPAQ 360

Query: 361 RITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG 420
           RITSA+VL+HPW+REGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG
Sbjct: 361 RITSAQVLDHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQG 420

Query: 421 LKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITA 480
           LK  F N+DTD SGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNG+IDYIEFITA
Sbjct: 421 LKTTFTNLDTDNSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGSIDYIEFITA 480

Query: 481 TMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADND 540
           TMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+YGMGD+ETIREIISEVDADND
Sbjct: 481 TMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADND 518

Query: 541 GRINYDEFCTMMRSGTQQAKLF 563
           GRINYDEFCTMMR+GTQQ KLF
Sbjct: 541 GRINYDEFCTMMRTGTQQGKLF 518

BLAST of Spg000138 vs. ExPASy TrEMBL
Match: A0A6J1FR98 (calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111447473 PE=4 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 482/564 (85.46%), Postives = 503/564 (89.18%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHH--SAAAKSTGAPKSPRHNILRSDT 60
           MGICTSKGKYSPQYDRYE QAK PAQH HRSPEN+H  + AAKS+G PKSPRHNILRSDT
Sbjct: 1   MGICTSKGKYSPQYDRYENQAKPPAQHAHRSPENYHPEATAAKSSGPPKSPRHNILRSDT 60

Query: 61  ILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRR 120
           ILGKP EDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVT+ DK+DIRR
Sbjct: 61  ILGKPFEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTKNDKDDIRR 120

Query: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180
           EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS
Sbjct: 121 EIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRS 180

Query: 181 IVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLH 240
           IVNVVHICHFMGVMHRDLKPENFLLASK+E+AMLKATDFGLSVFIE+             
Sbjct: 181 IVNVVHICHFMGVMHRDLKPENFLLASKEENAMLKATDFGLSVFIEE------------- 240

Query: 241 AFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDI 300
                                          GKVY+DIVGSAYYVAPEVLRR+YGKEIDI
Sbjct: 241 -------------------------------GKVYKDIVGSAYYVAPEVLRRRYGKEIDI 300

Query: 301 WSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDP 360
           WSAGVMLYILLSGVPPFWAETEKGIFD+ILQGH+DFE+DPWPKIS SAKDLVKKML  D 
Sbjct: 301 WSAGVMLYILLSGVPPFWAETEKGIFDSILQGHLDFETDPWPKISNSAKDLVKKMLNPDA 360

Query: 361 ARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420
           ++R+TSA+VLEHPWLREGG+ASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI
Sbjct: 361 SKRLTSAQVLEHPWLREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEI 420

Query: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480
           QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI
Sbjct: 421 QGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFI 480

Query: 481 TATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDAD 540
           TATMHRYKLEKEEHLYKAF HFDKDNSG+ITRDELKAAMK+Y MGD+ETI EIISEVDAD
Sbjct: 481 TATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYRMGDEETINEIISEVDAD 520

Query: 541 NDGRINYDEFCTMMRSGTQQAKLF 563
           NDGRINYDEFCTMMRSGTQQ KLF
Sbjct: 541 NDGRINYDEFCTMMRSGTQQVKLF 520

BLAST of Spg000138 vs. TAIR 10
Match: AT3G20410.1 (calmodulin-domain protein kinase 9 )

HSP 1 Score: 763.8 bits (1971), Expect = 9.4e-221
Identity = 389/556 (69.96%), Postives = 454/556 (81.65%), Query Frame = 0

Query: 10  YSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSPRHNILRSDTILGKPLEDVKL 69
           Y+PQ  R  QQ + P   + + P    +AAA    +PK+      +S++IL    EDVKL
Sbjct: 36  YTPQ-ARTTQQPEKPGSVNSQPPPWRAAAAAPGL-SPKT----TTKSNSILENAFEDVKL 95

Query: 70  HYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQHLSGQ 129
            YT+GKELGRGQFGVTYLCTENS+GK+YACKSISK+KLVT+ DK+D+RREI IMQHLSGQ
Sbjct: 96  FYTLGKELGRGQFGVTYLCTENSTGKKYACKSISKKKLVTKADKDDMRREIQIMQHLSGQ 155

Query: 130 PNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHICHFM 189
           PNIVEFKGAYED+++V+L+MELCAGGELFDRIIAKGHY+E+AAAS+CR IVNVV ICHFM
Sbjct: 156 PNIVEFKGAYEDEKAVNLVMELCAGGELFDRIIAKGHYTERAAASVCRQIVNVVKICHFM 215

Query: 190 GVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECLSVLV 249
           GV+HRDLKPENFLL+SKDE A++KATDFGLSVFIE+                        
Sbjct: 216 GVLHRDLKPENFLLSSKDEKALIKATDFGLSVFIEE------------------------ 275

Query: 250 LVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILL 309
                               GKVYRDIVGSAYYVAPEVLRR+YGKE+DIWSAG++LYILL
Sbjct: 276 --------------------GKVYRDIVGSAYYVAPEVLRRRYGKEVDIWSAGIILYILL 335

Query: 310 SGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSAEVLE 369
           SGVPPFWAETEKGIFDAIL+GHIDFES PWP IS+SAKDLV++MLT DP RRI++A+VL+
Sbjct: 336 SGVPPFWAETEKGIFDAILEGHIDFESQPWPSISSSAKDLVRRMLTADPKRRISAADVLQ 395

Query: 370 HPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFMNMD 429
           HPWLREGGEASDKPIDSAV +R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+D
Sbjct: 396 HPWLREGGEASDKPIDSAVLSRMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANID 455

Query: 430 TDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEK 489
           TD SGTITY ELK GLA+LGS L+EAEVKQLM+AADVDGNG+IDYIEFITATMHR++LE 
Sbjct: 456 TDNSGTITYEELKEGLAKLGSKLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLES 515

Query: 490 EEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINYDEFC 549
            E+LYKAF HFDKD+SG+IT DEL++A+KEYGMGDD TI+E++S+VD+DNDGRINY+EFC
Sbjct: 516 NENLYKAFQHFDKDSSGYITIDELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEEFC 541

Query: 550 TMMRSGT---QQAKLF 563
            MMRSG    QQ +LF
Sbjct: 576 AMMRSGNPQQQQPRLF 541

BLAST of Spg000138 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 760.8 bits (1963), Expect = 8.0e-220
Identity = 392/569 (68.89%), Postives = 455/569 (79.96%), Query Frame = 0

Query: 1   MGICTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAAKSTGAPKSP------RHNIL 60
           MG C +K KY     + +Q  +   + ++R    H   +  STG  + P      +H+  
Sbjct: 1   MGNCLAK-KYG-LVMKPQQNGERSVEIENRRRSTHQDPSKISTGTNQPPPWRNPAKHS-- 60

Query: 61  RSDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDKE 120
            +  IL KP EDVKL YT+ KELGRGQFGVTYLCTE S+GK++ACKSISK+KLVT+ DKE
Sbjct: 61  GAAAILEKPYEDVKLFYTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKE 120

Query: 121 DIRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAAS 180
           D+RREI IMQHLSGQPNIVEFKGAYED+++V+L+MELCAGGELFDRI+AKGHYSE+AAAS
Sbjct: 121 DMRREIQIMQHLSGQPNIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAAS 180

Query: 181 ICRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLIG 240
           +CR IVNVV+ICHFMGVMHRDLKPENFLL+SKDE A++KATDFGLSVFIE+         
Sbjct: 181 VCRQIVNVVNICHFMGVMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEE--------- 240

Query: 241 FVLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYGK 300
                                              G+VY+DIVGSAYYVAPEVL+R+YGK
Sbjct: 241 -----------------------------------GRVYKDIVGSAYYVAPEVLKRRYGK 300

Query: 301 EIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKML 360
           EIDIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G IDFES PWP IS SAKDLV++ML
Sbjct: 301 EIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRML 360

Query: 361 TQDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLS 420
           TQDP RRI++AEVL+HPWLREGGEASDKPIDSAV +R+KQFRAMNKLKKLALKVIAEN+ 
Sbjct: 361 TQDPKRRISAAEVLKHPWLREGGEASDKPIDSAVLSRMKQFRAMNKLKKLALKVIAENID 420

Query: 421 EEEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTIDY 480
            EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS L+EAEVKQLM+AADVDGNG+IDY
Sbjct: 421 TEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGSRLTEAEVKQLMDAADVDGNGSIDY 480

Query: 481 IEFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIISE 540
           IEFITATMHR++LE  E++YKAF HFDKD SG+IT DEL+AA+KEYGMGDD TI+EI+S+
Sbjct: 481 IEFITATMHRHRLESNENVYKAFQHFDKDGSGYITTDELEAALKEYGMGDDATIKEILSD 521

Query: 541 VDADNDGRINYDEFCTMMRSGT-QQAKLF 563
           VDADNDGRINYDEFC MMRSG  QQ +LF
Sbjct: 541 VDADNDGRINYDEFCAMMRSGNPQQPRLF 521

BLAST of Spg000138 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 739.6 bits (1908), Expect = 1.9e-213
Identity = 376/565 (66.55%), Postives = 436/565 (77.17%), Query Frame = 0

Query: 4   CTSKGKYSPQYDRYEQQAKVPAQHDHRSPENHHSAAA----------KSTGAPKSPRHNI 63
           C S      Q D  E+   +     H  PE+                +    P S   ++
Sbjct: 3   CFSSKHRKTQNDGGEKSIPINPVQTHVVPEHRKPQTPTPKPMTQPIHQQISTPSSNPVSV 62

Query: 64  LRSDTILGKPLEDVKLHYTIGKELGRGQFGVTYLCTENSSGKQYACKSISKRKLVTRTDK 123
              DTILGKP ED++  Y++GKELGRGQFG+TY+C E  +G  YACKSI KRKL+++ DK
Sbjct: 63  RDPDTILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDK 122

Query: 124 EDIRREIHIMQHLSGQPNIVEFKGAYEDKQSVHLIMELCAGGELFDRIIAKGHYSEKAAA 183
           ED++REI IMQ+LSGQPNIVE KGAYED+QS+HL+MELCAGGELFDRIIA+GHYSE+AAA
Sbjct: 123 EDVKREIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAA 182

Query: 184 SICRSIVNVVHICHFMGVMHRDLKPENFLLASKDEDAMLKATDFGLSVFIEDGEVLALLI 243
            I RSIVNVV ICHFMGV+HRDLKPENFLL+SK+E+AMLKATDFGLSVFIE+        
Sbjct: 183 GIIRSIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEE-------- 242

Query: 244 GFVLHAFLIECLSVLVLVDFAVLVNGFRRMQYYLRMGKVYRDIVGSAYYVAPEVLRRKYG 303
                                               GKVYRDIVGSAYYVAPEVLRR YG
Sbjct: 243 ------------------------------------GKVYRDIVGSAYYVAPEVLRRSYG 302

Query: 304 KEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPKISASAKDLVKKM 363
           KEIDIWSAGV+LYILLSGVPPFWAE EKGIFD +++G IDF S+PWP IS SAKDLV+KM
Sbjct: 303 KEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEPWPSISESAKDLVRKM 362

Query: 364 LTQDPARRITSAEVLEHPWLREGGEASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENL 423
           LT+DP RRIT+A+VLEHPW++ GGEA DKPIDSAV +R+KQFRAMNKLKKLALKVIAE+L
Sbjct: 363 LTKDPKRRITAAQVLEHPWIK-GGEAPDKPIDSAVLSRMKQFRAMNKLKKLALKVIAESL 422

Query: 424 SEEEIQGLKAMFMNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGTID 483
           SEEEI+GLK MF N+DTDKSGTITY ELK+GL RLGS LSE EVKQLMEAADVDGNGTID
Sbjct: 423 SEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLMEAADVDGNGTID 482

Query: 484 YIEFITATMHRYKLEKEEHLYKAFLHFDKDNSGHITRDELKAAMKEYGMGDDETIREIIS 543
           Y EFI+ATMHRYKL+++EH+YKAF HFDKDNSGHITRDEL++AMKEYGMGD+ +I+E+IS
Sbjct: 483 YYEFISATMHRYKLDRDEHVYKAFQHFDKDNSGHITRDELESAMKEYGMGDEASIKEVIS 522

Query: 544 EVDADNDGRINYDEFCTMMRSGTQQ 559
           EVD DNDGRIN++EFC MMRSG+ Q
Sbjct: 543 EVDTDNDGRINFEEFCAMMRSGSTQ 522

BLAST of Spg000138 vs. TAIR 10
Match: AT4G21940.1 (calcium-dependent protein kinase 15 )

HSP 1 Score: 715.7 bits (1846), Expect = 2.9e-206
Identity = 358/540 (66.30%), Postives = 425/540 (78.70%), Query Frame = 0

Query: 34  NHHSAAAKSTGAPKSPRHNILR---------SDTILGKPLEDVKLHYTIGKELGRGQFGV 93
           +HH   +K        +H + +         ++TILGKP E+++  YT+GKELGRGQFG+
Sbjct: 56  HHHQQESKPVNQQIEKKHVLTQPLKPIVFRETETILGKPFEEIRKLYTLGKELGRGQFGI 115

Query: 94  TYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQHLSGQPNIVEFKGAYEDKQS 153
           TY C ENS+G  YACKSI KRKL  + D +D++REI IMQ+LSGQ NIVE KGAYED+QS
Sbjct: 116 TYTCKENSTGNTYACKSILKRKLTRKQDIDDVKREIQIMQYLSGQENIVEIKGAYEDRQS 175

Query: 154 VHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHICHFMGVMHRDLKPENFLLA 213
           +HL+MELC G ELFDRIIA+GHYSEKAAA + RS++NVV ICHFMGV+HRDLKPENFLLA
Sbjct: 176 IHLVMELCGGSELFDRIIAQGHYSEKAAAGVIRSVLNVVQICHFMGVIHRDLKPENFLLA 235

Query: 214 SKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECLSVLVLVDFAVLVNGFRRMQ 273
           S DE+AMLKATDFGLSVFIE+                                       
Sbjct: 236 STDENAMLKATDFGLSVFIEE--------------------------------------- 295

Query: 274 YYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIF 333
                GKVYRDIVGSAYYVAPEVLRR YGKEIDIWSAG++LYILL GVPPFW+ETEKGIF
Sbjct: 296 -----GKVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGIILYILLCGVPPFWSETEKGIF 355

Query: 334 DAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSAEVLEHPWLREGGEASDKPI 393
           + I++G IDF+S PWP IS SAKDLV+K+LT+DP +RI++A+ LEHPW+R GGEA DKPI
Sbjct: 356 NEIIKGEIDFDSQPWPSISESAKDLVRKLLTKDPKQRISAAQALEHPWIR-GGEAPDKPI 415

Query: 394 DSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFMNMDTDKSGTITYAELKSG 453
           DSAV +R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF NMDTDKSGTITY ELK+G
Sbjct: 416 DSAVLSRMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITYEELKNG 475

Query: 454 LARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFLHFDKDN 513
           LA+LGS L+EAEVKQLMEAADVDGNGTIDYIEFI+ATMHRY+ +++EH++KAF +FDKDN
Sbjct: 476 LAKLGSKLTEAEVKQLMEAADVDGNGTIDYIEFISATMHRYRFDRDEHVFKAFQYFDKDN 535

Query: 514 SGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINYDEFCTMMRSG---TQQAKL 562
           SG IT DEL++AMKEYGMGD+ +I+E+I+EVD DNDGRINY+EFC MMRSG    QQ K+
Sbjct: 536 SGFITMDELESAMKEYGMGDEASIKEVIAEVDTDNDGRINYEEFCAMMRSGITLPQQGKI 550

BLAST of Spg000138 vs. TAIR 10
Match: AT4G21940.2 (calcium-dependent protein kinase 15 )

HSP 1 Score: 715.7 bits (1846), Expect = 2.9e-206
Identity = 358/540 (66.30%), Postives = 425/540 (78.70%), Query Frame = 0

Query: 34  NHHSAAAKSTGAPKSPRHNILR---------SDTILGKPLEDVKLHYTIGKELGRGQFGV 93
           +HH   +K        +H + +         ++TILGKP E+++  YT+GKELGRGQFG+
Sbjct: 56  HHHQQESKPVNQQIEKKHVLTQPLKPIVFRETETILGKPFEEIRKLYTLGKELGRGQFGI 115

Query: 94  TYLCTENSSGKQYACKSISKRKLVTRTDKEDIRREIHIMQHLSGQPNIVEFKGAYEDKQS 153
           TY C ENS+G  YACKSI KRKL  + D +D++REI IMQ+LSGQ NIVE KGAYED+QS
Sbjct: 116 TYTCKENSTGNTYACKSILKRKLTRKQDIDDVKREIQIMQYLSGQENIVEIKGAYEDRQS 175

Query: 154 VHLIMELCAGGELFDRIIAKGHYSEKAAASICRSIVNVVHICHFMGVMHRDLKPENFLLA 213
           +HL+MELC G ELFDRIIA+GHYSEKAAA + RS++NVV ICHFMGV+HRDLKPENFLLA
Sbjct: 176 IHLVMELCGGSELFDRIIAQGHYSEKAAAGVIRSVLNVVQICHFMGVIHRDLKPENFLLA 235

Query: 214 SKDEDAMLKATDFGLSVFIEDGEVLALLIGFVLHAFLIECLSVLVLVDFAVLVNGFRRMQ 273
           S DE+AMLKATDFGLSVFIE+                                       
Sbjct: 236 STDENAMLKATDFGLSVFIEE--------------------------------------- 295

Query: 274 YYLRMGKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIF 333
                GKVYRDIVGSAYYVAPEVLRR YGKEIDIWSAG++LYILL GVPPFW+ETEKGIF
Sbjct: 296 -----GKVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGIILYILLCGVPPFWSETEKGIF 355

Query: 334 DAILQGHIDFESDPWPKISASAKDLVKKMLTQDPARRITSAEVLEHPWLREGGEASDKPI 393
           + I++G IDF+S PWP IS SAKDLV+K+LT+DP +RI++A+ LEHPW+R GGEA DKPI
Sbjct: 356 NEIIKGEIDFDSQPWPSISESAKDLVRKLLTKDPKQRISAAQALEHPWIR-GGEAPDKPI 415

Query: 394 DSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFMNMDTDKSGTITYAELKSG 453
           DSAV +R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF NMDTDKSGTITY ELK+G
Sbjct: 416 DSAVLSRMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITYEELKNG 475

Query: 454 LARLGSTLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFLHFDKDN 513
           LA+LGS L+EAEVKQLMEAADVDGNGTIDYIEFI+ATMHRY+ +++EH++KAF +FDKDN
Sbjct: 476 LAKLGSKLTEAEVKQLMEAADVDGNGTIDYIEFISATMHRYRFDRDEHVFKAFQYFDKDN 535

Query: 514 SGHITRDELKAAMKEYGMGDDETIREIISEVDADNDGRINYDEFCTMMRSG---TQQAKL 562
           SG IT DEL++AMKEYGMGD+ +I+E+I+EVD DNDGRINY+EFC MMRSG    QQ K+
Sbjct: 536 SGFITMDELESAMKEYGMGDEASIKEVIAEVDTDNDGRINYEEFCAMMRSGITLPQQGKI 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903170.11.7e-27786.77calcium-dependent protein kinase 2-like [Benincasa hispida][more]
XP_023520424.14.8e-27585.59calcium-dependent protein kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_008463413.15.3e-27486.50PREDICTED: calcium-dependent protein kinase-like [Cucumis melo] >KAA0033976.1 ca... [more]
KAG6600782.19.0e-27485.64Calcium-dependent protein kinase 2, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7031419.11.2e-27385.46Calcium-dependent protein kinase 9 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q388681.3e-21969.96Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 ... [more]
Q9C6P31.1e-21868.89Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
P285825.6e-21868.36Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
P491011.5e-21569.44Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1[more]
P536837.5e-21569.68Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7SVI62.5e-27486.50Calcium-dependent protein kinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CJN02.5e-27486.50calcium-dependent protein kinase-like OS=Cucumis melo OX=3656 GN=LOC103501584 PE... [more]
A0A6J1IGJ91.3e-27385.46calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111476... [more]
A0A6J1F0W01.7e-27385.23calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1FR981.7e-27385.46calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G20410.19.4e-22169.96calmodulin-domain protein kinase 9 [more]
AT1G50700.18.0e-22068.89calcium-dependent protein kinase 33 [more]
AT4G04720.11.9e-21366.55calcium-dependent protein kinase 21 [more]
AT4G21940.12.9e-20666.30calcium-dependent protein kinase 15 [more]
AT4G21940.22.9e-20666.30calcium-dependent protein kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 492..520
e-value: 2.5E-4
score: 30.4
coord: 527..555
e-value: 2.4E-7
score: 40.4
coord: 456..484
e-value: 0.032
score: 23.4
coord: 420..448
e-value: 1.8E-4
score: 30.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 490..553
e-value: 2.1E-14
score: 53.7
coord: 421..481
e-value: 1.2E-10
score: 41.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 524..558
score: 12.532831
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 416..451
score: 14.318138
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 452..487
score: 11.361225
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 488..523
score: 15.740803
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 71..373
e-value: 1.6E-92
score: 323.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 71..373
e-value: 4.2E-69
score: 232.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 71..373
score: 47.42453
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 72..156
e-value: 5.5E-30
score: 105.5
NoneNo IPR availableGENE3D1.10.238.10coord: 381..558
e-value: 1.4E-59
score: 202.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 157..380
e-value: 2.1E-54
score: 185.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..53
NoneNo IPR availablePANTHERPTHR24349:SF174CALCIUM-DEPENDENT PROTEIN KINASE 21-RELATEDcoord: 263..557
coord: 29..234
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 263..557
coord: 29..234
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 70..372
e-value: 8.84939E-129
score: 375.276
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 77..100
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 191..203
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 429..441
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 501..513
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 465..477
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 536..548
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 70..381
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 412..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000138.1Spg000138.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity