Spg000077 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000077
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionlactoylglutathione lyase-like
Locationscaffold6: 23563135 .. 23568106 (-)
RNA-Seq ExpressionSpg000077
SyntenySpg000077
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAATCCTTTTCATTACACTAAACATTCATAGCCTTCCACTTTGAAGATGGCCAACAAGTTGGCACCTTTTTCAATCCTCTGCTCTCTTCTTTTCTTCTCTTTGGTAAGCTTTCTTTCTGGTTCTTAGCTTCTCTCCAGCTTTTCCGATCAGAGGCCATATTTAACATGAAAAATTGCTTGACCTAATTGTCAGATCATCGGAACCAGTTTGGCAGCTCGTAATTTGAATGAAAATGCGTTGGAATGGGTGAAGAGAGACCATCGTCGTTTTCTTCGTGCTGTTATTCATGTCTCCAATCTCGACCGCTCTATCAAGTAGGTATATATAAGCTTTTGATTTTGTGTGGTTTAAAATGTTTGTTTCCATGGCTCAAAATTTTGAACCTGCAGATTTTACACGCAAGCCTTTGGAATGAAACTTTTGAAGACAAGGAACTTCCCAGATCGTCAATACAGAGACGCCCTCGTTGGTTTTGGGAGCGAAAGTTCCCACTTCTTGGTAGAGCTAAGACAACGTGAGTTTATAAATTTGAACTCTTAAATCTTGGTTCCAAGAGCTCAAATTTTGAGTTCCCAACTTGCAGGGCATGATTCAAGCGATGTATTTATTGGGACGGAGTTTGGGCACTTCGGCATTGCCACTCAAGATGTGGGTAATTTTTATTTTTATATTTTTTAACAACTGTGTTTGTAATTGTAATTTCCTATAATGAATTTGGTGACCATGGCTTTATATGTAGGTCTACAAGTCTGTGGAGAAAGCTCGAGCGAATGGCGCAGTGGTGATCCACAAACCCCAAAGGGTTAACAAAAGCATACTTGCGTTTGTGCAAGATCGTGATGGTTATATGTTTAAGCTGATTCAGAGGTCCCCACCAGCTGATCCTCTTTGTGAAATTATGCTTCATGTACAAGATTTGAATCGGGCCCTCACCTTTTATACCAAGGTAAACAAACTTAGATTGATGCCATTTAATTTTGGTAAATGTTAAAACAAGTTAAATTTTGGTACACAAAAATATCAAATAGGAGATATTATCCATTAAATGTTGATTTAAGAAGCATATATATATATATATATATGAAAAATCAATCCATCAATATATGTATATATTTATTTTAACCAGTGAACAAAGGTCACTTTTAATTAATGAATAAAGATTCATTTTTCTTTTTTTTTTTTTTTATGAAAGTTATAAATTGTTATTACTGAAATATATATTTAGTGATATTTCTGTTGAAAATGTTAATATATCTTAATATTTCTTTTTGGTGTCCACATTTCGTAGTAGTTCAATCTTCGGAGAAAAAAAAAAAACTTTTCGTACTTCTCTCCTTTAAAGTAAAAGACTTTTTTTTTTAAAAAAAAATAATAATTTATTGTTTTGAGTCAATACATTACAAAGATATTAATTGAACTTCGATTCATTGCCAAGCGATTAGATGTACGGGGCTATTTTTTTCTTTTTCATTTTTCTTTAGTTTATCAATTTTTATGAAATAATTCTTTTAAGTATTATTTAGAATACAAATAATGTACATTGAAAAAAAAAATACATTGTTGAAATATTATTCGAATATTGTAGCAGTTTTTTTTTAAAACATTTTATTTTTAGAGGAAATATTTAAGCAATTGCCTTACGCGTTGCGAAATCTTAATGCAGGCATTGGGAATGAAGTTGTTGAAGAGGCAAAACAATTCAACAGGACAGGTAAAGAACTACCAATTACCATATATATGTTATTTCTCAATTTAAAGAAAAATTATATTAATGAGATTCCAATGCTAACTTATAATAAAATTTTTGAAATTTTGTTGGTTATTATTTTGTTGGGCAGTTTGTTTGGGGGACTTTGGGTTATGGAGCAAACCAAAGTAAAACAACAGTATTGCAGTTGGAAAAGAGAAGGAACGTCCCTCGTGATGATGGACGAGATGGTTATTCAATGGTATGCCCACATCCTTAAGCACTATATTTTATTTTTTCAATTATATATCATATCTTATTTACTCTAATTCTTCCTTTATTATTAATCTTTTTTATTAACATCTCACTGTGAGCTTATAATTCAATAGTTATGGTATAATATAATTGAACTTACTTTACCAAATAACACATCTATTACCATTGAAAATATCGGTGATTTAATCCTACACCCTGAAATTTTATAGCTCAAAAAAGAAAAAAAAAAACTTTTCTCTACGGATCAAAGGTTCCAATTATCTACCTCCACGTTTATAATACTATATTCTAAAGAAAAAAAAATAGAAAATGTAGGTGGAATTATAATATTAACGTATTATGCTGTTGATGAAGTTAAATGAGTAGATGGAAATTAAAAAATGCTAGAAAATATATACTAAGAAGTGTAAATTAAAGACTATGATGAAATGAAAGAGAAGAAAAAATTCAATCTCGTGTTATAATAATAACAACAATAATAACAAAAGAGAAAAAAAATTAGCTCAAGAATTATTCTAGCTTGTTAGGTAGACCTCCCTCGAAAATTACAATTTCATAAGTTTTAGCCTTTTTTTTCCGAAGAAATTCGACTTGTTTGCTCTTGTCACACTGTTGTCGTAAAAACATATTTTATGTTTATAGGAAGATTTTAGAAAACAAAAAATAGCAATTTGAAAAGTAATTAATGATATTATGATTGTTATTTTCCATTTTTCAGCTGTATATTGGTACTGACAACGTAAACAAGAGTGCTGAAGCTACTAAGGTTGTAATCAAAGAGGTTGGTGGTAACCTAATCATGGAGCCGGTTCTTATACCAGATATCAATGTCAAACTAACTAGCTTTAGTGACCCAGATAATTGGATTACTGTAAGTACACCTCATTATTACCTTCTTTAAATTTTCTATATTGTTTTTGTCCAATTCTATTTTCAATTAAATTAGTCTCCTTTGACAACTATTTGGCTTTTGGTTTTTGGTTTTTAAAATTATGTTTGTTTTCTCTTAAATTTCCTATCATGACTTTCATCATAGTTAAGGTTGCATTTGAATTCCTAACCAAATTTTAAAACAAAAATGTGTTTTTGAAAACTATTTTTTTTCCAAAATTGGACTTGGTTTTTGAAAACAATGTAGAATATTGATACTAAAATCAAGAAACTTATAGGTGGAAGTATGTGTGTATAAGCTTAATTTTCAAAAACCAAAAACAAAAAATCAAATAGACATGTGCAGTGCATATATTCGACTCTTTTTTTTAAAGAAATATATTTGACTCATTTTAATTCTTATAATTCTTACCCCATAATTTTCTTCTTTTCTTCTCAGATTATGGTGGACAATAAAGATTACCAAAGAGGAACTCTTTAAGAAAAAAAAAAAAAAAAAAACAAGAATAATGGATGACAATACTCGAATTCAGACGATCGTGTACATTTCCTAGAAGACGAGTTCATTGCTTAATTGGGTGTTGTCTTTATTATAATAGTCTAGTGTTGTGTTATTATTGCGCTTGTGTATTTATATATGTTCTTTCCACAAATAAGATTTTCAATTCTTGGTTAATGGCTGTTGATGTACTCTTCAGTGAAAGCATTAAAGTCGATGGAATTATTTTTGTTATCATTCCCTTCCTAGAACGGTCAATTTGTATTTGGAAGTAGTGGCGGGTCGAAAACTCAAGGGCTCGATAGATTTTTAATTAAATTACAAATTTGTTATATGGTTTAGATTAAGTTATAATTTAGTCTCTATGGTTTGATAAAACCTCATTAAATAGTTCTTACCATTAGAAACGATTGAGCACATGGTTCTTAGAGGTAGGATAGGAAAAAATTTTTAATAGATTGAGTATGTGAAAGAACTTATGGATAGATTCACTGGAGCACACTCATCGAATTGAAGGTATGATTTTTAAAACTTTGAATTTAAGTTCATTTTTTAGGTTGGGACTCTGATTACATTGTTTGACTATGTTTTCATATGTTTTGTGACTTTGGGGAGAGTGTTTGTTCTTGAGGATGAATTGAGGGGGGGAGGTGTTTTCAACTTGAGGATGAATTGAGGATGAGAGGGGGTTGGTTGTTCATTATAGATTGTCAAATATATCTTTTTCAAATGTTTTTATTGTGTTTTAAGGAGGGGGAGTAATATTGGTGATTAGAACTTGGAATTTAATTTCATTTTTTAGGTTGGGACTCTGATTGCACTGTTTGAGACTATGTTTTCATATGTTTTGTGCCTATGTATGTGTGATTCTCTGAATGAGAATGGATGTTATATTTTTTTTGTGGTTGACTGTGGGAAGAGTGTTTGTTCTTGAGGATGAATTGAGGGAGGGGGGAGTGTGTTTTCAACTTGAGGATGAATTGAGGAGGAGAGGGGGTTGGTTGTTCATTATAGATATTGTCAACTATGTCTTTTTCAAAAGTTTTTATTGTGTTTTGAGGAGGGGGTGCAATATTGATGATTAGAACATGGAATTTAAGTTCATTTTTTAGGTTGGAACTCTGATTGCACTGTTTGAGGTTCTATTTAAGTTGTCCAGGTGGTTGTTCTTGAGAAATAACTTTTTAACTTAAGTTGGGTGAAAAGTTTTCAACGAAAACCTTATAATTGTAATTGTAACTTAATATGCTCTTTCTGAATATGGTTAAGGATGATTTGTTGTTCATGTGTACTTATGAGTATGTAATATGTTGTTTATTTGCAGGATTGAAGTTAACTTGCTGATGACAACAATGACCAAATGAACATTACTTTTTTGGAGCTAACATAGACTTAGTACATGTCTGTATTTGTATTTGGATACTTGTTTGGTCTTTATTTTTTGGAACTCTTAAATTTAGCAAGGCTTTGGGTAAAATGTACATATGTGTGAAACATAATTTTGTTGAATGCTTATATATTGTATAAAGTGGATGAATGCTTTAGTAAAATATTTTTATATGTTCAATGCATATTTGTATAAAATGTAAATCAGGACAATTAAAATGAAATCGAGC

mRNA sequence

ATGGCCAACAAGTTGGCACCTTTTTCAATCCTCTGCTCTCTTCTTTTCTTCTCTTTGATCATCGGAACCAGTTTGGCAGCTCGTAATTTGAATGAAAATGCGTTGGAATGGGTGAAGAGAGACCATCGTCGTTTTCTTCGTGCTGTTATTCATGTCTCCAATCTCGACCGCTCTATCAAATTTTACACGCAAGCCTTTGGAATGAAACTTTTGAAGACAAGGAACTTCCCAGATCGTCAATACAGAGACGCCCTCGTTGGTTTTGGGAGCGAAAGTTCCCACTTCTTGGTAGAGCTAAGACAACGGCATGATTCAAGCGATGTATTTATTGGGACGGAGTTTGGGCACTTCGGCATTGCCACTCAAGATGTCTACAAGTCTGTGGAGAAAGCTCGAGCGAATGGCGCAGTGGTGATCCACAAACCCCAAAGGGTTAACAAAAGCATACTTGCGTTTGTGCAAGATCGTGATGGTTATATGTTTAAGCTGATTCAGAGGTCCCCACCAGCTGATCCTCTTTGTGAAATTATGCTTCATGTACAAGATTTGAATCGGGCCCTCACCTTTTATACCAAGGCATTGGGAATGAAGTTGTTGAAGAGGCAAAACAATTCAACAGGACAGTTTGTTTGGGGGACTTTGGGTTATGGAGCAAACCAAAGTAAAACAACAGTATTGCAGTTGGAAAAGAGAAGGAACGTCCCTCGTGATGATGGACGAGATGGTTATTCAATGCTGTATATTGGTACTGACAACGTAAACAAGAGTGCTGAAGCTACTAAGGTTGTAATCAAAGAGGTTGGTGGTAACCTAATCATGGAGCCGGTTCTTATACCAGATATCAATGTCAAACTAACTAGCTTTAGTGACCCAGATAATTGGATTACTATTATGGTGGACAATAAAGATTACCAAAGAGGAACTCTTTAA

Coding sequence (CDS)

ATGGCCAACAAGTTGGCACCTTTTTCAATCCTCTGCTCTCTTCTTTTCTTCTCTTTGATCATCGGAACCAGTTTGGCAGCTCGTAATTTGAATGAAAATGCGTTGGAATGGGTGAAGAGAGACCATCGTCGTTTTCTTCGTGCTGTTATTCATGTCTCCAATCTCGACCGCTCTATCAAATTTTACACGCAAGCCTTTGGAATGAAACTTTTGAAGACAAGGAACTTCCCAGATCGTCAATACAGAGACGCCCTCGTTGGTTTTGGGAGCGAAAGTTCCCACTTCTTGGTAGAGCTAAGACAACGGCATGATTCAAGCGATGTATTTATTGGGACGGAGTTTGGGCACTTCGGCATTGCCACTCAAGATGTCTACAAGTCTGTGGAGAAAGCTCGAGCGAATGGCGCAGTGGTGATCCACAAACCCCAAAGGGTTAACAAAAGCATACTTGCGTTTGTGCAAGATCGTGATGGTTATATGTTTAAGCTGATTCAGAGGTCCCCACCAGCTGATCCTCTTTGTGAAATTATGCTTCATGTACAAGATTTGAATCGGGCCCTCACCTTTTATACCAAGGCATTGGGAATGAAGTTGTTGAAGAGGCAAAACAATTCAACAGGACAGTTTGTTTGGGGGACTTTGGGTTATGGAGCAAACCAAAGTAAAACAACAGTATTGCAGTTGGAAAAGAGAAGGAACGTCCCTCGTGATGATGGACGAGATGGTTATTCAATGCTGTATATTGGTACTGACAACGTAAACAAGAGTGCTGAAGCTACTAAGGTTGTAATCAAAGAGGTTGGTGGTAACCTAATCATGGAGCCGGTTCTTATACCAGATATCAATGTCAAACTAACTAGCTTTAGTGACCCAGATAATTGGATTACTATTATGGTGGACAATAAAGATTACCAAAGAGGAACTCTTTAA

Protein sequence

MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDYQRGTL
Homology
BLAST of Spg000077 vs. NCBI nr
Match: XP_008463405.1 (PREDICTED: lactoylglutathione lyase-like [Cucumis melo])

HSP 1 Score: 513.8 bits (1322), Expect = 1.0e-141
Identity = 250/310 (80.65%), Postives = 283/310 (91.29%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MAN L PFSI+ SLLF SLIIGTSLAARNLN+N LEWVK+DHR FLRAVIHVS+LDRSI+
Sbjct: 1   MANNLGPFSIVSSLLFLSLIIGTSLAARNLNDNVLEWVKKDHRHFLRAVIHVSDLDRSIR 60

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYT+ FGMK+LK RNFP+RQYRDALVGFG +++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 61  FYTKGFGMKVLKRRNFPERQYRDALVGFGPQNTHFLLELRQRHDSNNVFIGTEFGHFGIA 120

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKSVEKARANGA+VI KPQ+VN+++ AFVQD DGY FKLIQR   ADPL ++MLHV
Sbjct: 121 TQDVYKSVEKARANGALVIQKPQKVNQTMFAFVQDHDGYKFKLIQRISFADPLVQVMLHV 180

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSK-TTVLQLEKRRNVPRDDG 240
           +DLNR+L FYTKALGMKL ++QNNSTGQ V GTLGYG NQSK TTVLQLEKR+N+PRDDG
Sbjct: 181 EDLNRSLNFYTKALGMKLFEKQNNSTGQIVLGTLGYGINQSKTTTVLQLEKRKNIPRDDG 240

Query: 241 RDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMV 300
           RDGYSM+YIGTDNVNKSAEA K+V+KE+GGNLI+EPVL+ DINVKLT FSDPDNWITIMV
Sbjct: 241 RDGYSMVYIGTDNVNKSAEAAKLVMKELGGNLIIEPVLLSDINVKLTGFSDPDNWITIMV 300

Query: 301 DNKDYQRGTL 310
           DNKDYQRG L
Sbjct: 301 DNKDYQRGRL 310

BLAST of Spg000077 vs. NCBI nr
Match: XP_038903299.1 (lactoylglutathione lyase-like [Benincasa hispida])

HSP 1 Score: 508.4 bits (1308), Expect = 4.2e-140
Identity = 244/309 (78.96%), Postives = 278/309 (89.97%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MANKL P SI  SLLFFSLIIGT+LAARNLN+N  EW+K+DHR   RAVIHVS+LDRSI+
Sbjct: 1   MANKLGPSSIFSSLLFFSLIIGTTLAARNLNDNVFEWLKKDHRGLPRAVIHVSDLDRSIR 60

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FY Q FGMKLLK RNFPDRQYRDAL+GFG +++HFL+ELRQR+DS+DVFIGTEFGHFGIA
Sbjct: 61  FYMQGFGMKLLKRRNFPDRQYRDALIGFGPQNTHFLLELRQRYDSNDVFIGTEFGHFGIA 120

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQD+YK+VEKARANGA+VIHKPQ+VN++I AFV+D DGY FKLIQR+  +DPL EIMLHV
Sbjct: 121 TQDIYKTVEKARANGALVIHKPQKVNQTIFAFVEDHDGYKFKLIQRTTSSDPLVEIMLHV 180

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           QDLN +L FYTKALGMKL +RQNNSTGQ V GTLGYG N SKTT+LQLEKR+N+PRDDGR
Sbjct: 181 QDLNTSLNFYTKALGMKLFERQNNSTGQIVLGTLGYGVNPSKTTILQLEKRKNIPRDDGR 240

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSM+YIGTDNVNKSAEA K+V+KE+GGNLI+EPVL  +INVKLT F+DPDNWIT MVD
Sbjct: 241 DGYSMVYIGTDNVNKSAEAAKLVMKELGGNLIIEPVLFSNINVKLTGFTDPDNWITNMVD 300

Query: 301 NKDYQRGTL 310
           NKDYQRGTL
Sbjct: 301 NKDYQRGTL 309

BLAST of Spg000077 vs. NCBI nr
Match: XP_022151011.1 (lactoylglutathione lyase-like [Momordica charantia])

HSP 1 Score: 507.3 bits (1305), Expect = 9.4e-140
Identity = 246/309 (79.61%), Postives = 273/309 (88.35%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           M N   P SI  SLLFFSLIIGT+LAARNLNEN LEWVKRDHRRFLRAVIHVS+LD S++
Sbjct: 2   MPNNFVP-SIFASLLFFSLIIGTTLAARNLNENVLEWVKRDHRRFLRAVIHVSDLDDSVR 61

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYTQ FGMK++K RNFPDRQYRDALVGFG E++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 62  FYTQGFGMKVVKRRNFPDRQYRDALVGFGPENTHFLLELRQRHDSNNVFIGTEFGHFGIA 121

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKS+EKAR NGAVVIH+PQ+VN +I A VQDRDGY FKLIQR P ADPLCE+ML V
Sbjct: 122 TQDVYKSLEKARVNGAVVIHEPQKVNNTIFALVQDRDGYKFKLIQRPPTADPLCEVMLRV 181

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           +DLNR++ FY KALGMKL KRQNNS GQF  GTLGYG +QSKTTVLQ EKR N+ RDDGR
Sbjct: 182 EDLNRSVNFYAKALGMKLFKRQNNSKGQFASGTLGYGTDQSKTTVLQFEKRNNISRDDGR 241

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSMLYI TDNVNKSAEA KVVIK++GGNLI+EP L+P INVK+T FSDPDNWI IMVD
Sbjct: 242 DGYSMLYISTDNVNKSAEAAKVVIKQLGGNLIIEPGLLPSINVKITGFSDPDNWIMIMVD 301

Query: 301 NKDYQRGTL 310
           NKDY+RGTL
Sbjct: 302 NKDYRRGTL 309

BLAST of Spg000077 vs. NCBI nr
Match: XP_011650522.1 (lactoylglutathione lyase [Cucumis sativus] >KGN56115.1 hypothetical protein Csa_009993 [Cucumis sativus])

HSP 1 Score: 504.6 bits (1298), Expect = 6.1e-139
Identity = 240/309 (77.67%), Postives = 280/309 (90.61%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MAN L PFSI+ SLLF SLIIGTSLAARNLN+N LEWVK+DHR FLRAVIHVS+LDRSI+
Sbjct: 1   MANNLGPFSIISSLLFLSLIIGTSLAARNLNDNVLEWVKKDHRHFLRAVIHVSDLDRSIR 60

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYT+ FGMK+LK RNFPDRQYRDALVGFG E++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 61  FYTKGFGMKVLKRRNFPDRQYRDALVGFGPENTHFLLELRQRHDSNNVFIGTEFGHFGIA 120

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKSVEKARANGA+VI KPQ++N+++ AFVQD DGY FKLIQ    ADPL ++M HV
Sbjct: 121 TQDVYKSVEKARANGALVIQKPQKINQTMFAFVQDHDGYKFKLIQSKCLADPLVQVMFHV 180

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           QDLNR++ FYTKALGMKL +++NNSTGQ V GTLGYG NQSKTTVLQLEKR+N+PRDDGR
Sbjct: 181 QDLNRSINFYTKALGMKLFEKKNNSTGQIVSGTLGYGINQSKTTVLQLEKRKNIPRDDGR 240

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSM+YIGTDNVNKSA+A K+V+KE+GG++I+EP+L+ +INVKLT F DPDNWITIMVD
Sbjct: 241 DGYSMVYIGTDNVNKSADAAKLVMKELGGSVIIEPILLSNINVKLTGFFDPDNWITIMVD 300

Query: 301 NKDYQRGTL 310
           NKDY++G L
Sbjct: 301 NKDYRKGRL 309

BLAST of Spg000077 vs. NCBI nr
Match: KAA0034003.1 (lactoylglutathione lyase-like [Cucumis melo var. makuwa] >TYK02802.1 lactoylglutathione lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 235/290 (81.03%), Postives = 268/290 (92.41%), Query Frame = 0

Query: 21  IGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQ 80
           +GTSLAARNLN+N LEWVK+DHRRFLRAVIHVS+LDRSI+FYT+ FGMK+LK RNFP+RQ
Sbjct: 1   MGTSLAARNLNDNVLEWVKKDHRRFLRAVIHVSDLDRSIRFYTKGFGMKVLKRRNFPERQ 60

Query: 81  YRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIH 140
           YRDALVGFG +++HFL+ELRQRHDS++VFIGTEFGHFGIATQDVYKSVEKARANGA+VI 
Sbjct: 61  YRDALVGFGPQNTHFLLELRQRHDSNNVFIGTEFGHFGIATQDVYKSVEKARANGALVIQ 120

Query: 141 KPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLK 200
           KPQ+VN+++ AFVQD DGY FKLIQR   ADPL ++MLHV+DLNR+L FYTKALGMKL +
Sbjct: 121 KPQKVNQTMFAFVQDHDGYKFKLIQRISFADPLVQVMLHVEDLNRSLNFYTKALGMKLFE 180

Query: 201 RQNNSTGQFVWGTLGYGANQSK-TTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEA 260
           +QNNSTGQ V GTLGYG NQSK TTVLQLEKR+N+PRDDGRDGYSM+YIGTDNVNKSAEA
Sbjct: 181 KQNNSTGQIVLGTLGYGINQSKTTTVLQLEKRKNIPRDDGRDGYSMVYIGTDNVNKSAEA 240

Query: 261 TKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDYQRGTL 310
            K+V+KE+GGNLI+EPVL+ DINVKLT FSDPDNWITIMVDNKDYQRG L
Sbjct: 241 AKLVMKELGGNLIIEPVLLSDINVKLTGFSDPDNWITIMVDNKDYQRGRL 290

BLAST of Spg000077 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.4e-67
Identity = 128/272 (47.06%), Postives = 180/272 (66.18%), Query Frame = 0

Query: 35  LEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSH 94
           LEW K+D+RRFL  V  V +LDR+I+FYT+ FGMKLL+ R+ P+ +Y +A +GFG E+S+
Sbjct: 8   LEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSN 67

Query: 95  FLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKSILAF 154
           F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S++AF
Sbjct: 68  FVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVIAF 127

Query: 155 VQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWG 214
           V+D DGY F+LIQR P  +P C++ML V DL+RA+ FY KALGM+LL++      ++  G
Sbjct: 128 VKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIG 187

Query: 215 TLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLI 274
            +GY A + ++ VL+L    +V      + Y+ + IGTD+V KS E  K+V +E+GG + 
Sbjct: 188 MMGY-AEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKIT 247

Query: 275 MEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
            E   +P +  K+ SF DPD W T++VDNKD+
Sbjct: 248 REAGPLPGLGTKIVSFLDPDGWKTVLVDNKDF 278

BLAST of Spg000077 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 9.3e-66
Identity = 124/276 (44.93%), Postives = 182/276 (65.94%), Query Frame = 0

Query: 32  ENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSE 91
           E  LEW K+D +R L AV  V +LDR+IK YT+ FGMKLL+ R+ P+ +Y +A +GFG E
Sbjct: 12  EVVLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPE 71

Query: 92  SSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHK---PQRVNKS 151
            ++F +EL   +      IG  FGHF IAT+DVYK  EK +++    I +   P +   +
Sbjct: 72  DTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGST 131

Query: 152 ILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQ 211
           ++AF QD DGYMF+LIQR P  +PLC++ML V DL+R++ FY KALGMKLL++++    +
Sbjct: 132 VIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYK 191

Query: 212 FVWGTLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVG 271
           +    LGY A++ KTTV++L     V      + Y+ + IGT++V KSAEA ++V KE+G
Sbjct: 192 YTIAMLGY-ADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELG 251

Query: 272 GNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
           G ++ +P  +P +N K+ SF DPD W  ++VDN D+
Sbjct: 252 GKILRQPGPLPGLNTKIASFLDPDGWKVVLVDNADF 286

BLAST of Spg000077 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.0e-64
Identity = 127/276 (46.01%), Postives = 179/276 (64.86%), Query Frame = 0

Query: 31  NENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGS 90
           N + +EW K+D RRFL  V  V +LDR+I+FYT+ FGMK+L+ R+ P+ +Y +A +GFG 
Sbjct: 4   NADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGP 63

Query: 91  ESSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKS 150
           E+S+F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S
Sbjct: 64  ETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGS 123

Query: 151 ILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQ 210
           ++AFV+D DGY F+LIQR P  +PLC++ML V DL+RA+ F  KALGM+LL+R       
Sbjct: 124 VIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN 183

Query: 211 FVWGTLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVG 270
            + G +GY A + ++ VL+L     V      + Y+ + IGTD+V KSAE  K+V +E+G
Sbjct: 184 TI-GMMGY-AEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELG 243

Query: 271 GNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
           G +  E   +P +  K+ SF DPD W  ++VDN+D+
Sbjct: 244 GKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNEDF 277

BLAST of Spg000077 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.1e-62
Identity = 121/281 (43.06%), Postives = 175/281 (62.28%), Query Frame = 0

Query: 26  AARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDAL 85
           A     ++ L WVK D RR L  V  V ++DR+IKFYT+  GMKLL+ R+ P+ +Y +A 
Sbjct: 70  AQATTQDDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAF 129

Query: 86  VGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV 145
           +G+G E SHF++EL   +      IG  FGHFGIA  DV K+VE  +A G  V  +P  V
Sbjct: 130 LGYGPEDSHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPV 189

Query: 146 --NKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQN 205
              K+++AF++D DGY F+L++R P  +PLC++ML V DL+RA+ FY KA GM+LL+ ++
Sbjct: 190 KGGKTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRD 249

Query: 206 NSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVV 265
           N   ++    +GYG  + K  VL+L     V   D  + Y+ + IGTD+V K+AEA    
Sbjct: 250 NPEYKYTIAMMGYGP-EDKFPVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEA---- 309

Query: 266 IKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
           IK  GG +  EP  +P I+ K+T+  DPD W ++ VDN D+
Sbjct: 310 IKLFGGKITREPGPLPGISTKITACLDPDGWKSVFVDNIDF 345

BLAST of Spg000077 vs. ExPASy Swiss-Prot
Match: Q55595 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=gloA PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-19
Identity = 44/128 (34.38%), Postives = 74/128 (57.81%), Query Frame = 0

Query: 46  LRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDS 105
           L  +I V +LD+S++FY    GM LL+ +++P  ++  A VG+G ES + ++EL     +
Sbjct: 4   LHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGT 63

Query: 106 SDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHK--PQRVNKSILAFVQDRDGYMFKL 165
               +G  FGH  +  +D+Y + +K R  G  V+ +  P +   +++AFV+D DGY  +L
Sbjct: 64  DKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIEL 123

Query: 166 IQRSPPAD 172
           IQ S   D
Sbjct: 124 IQTSSKKD 131

BLAST of Spg000077 vs. ExPASy TrEMBL
Match: A0A1S3CJ49 (Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103501576 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.8e-142
Identity = 250/310 (80.65%), Postives = 283/310 (91.29%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MAN L PFSI+ SLLF SLIIGTSLAARNLN+N LEWVK+DHR FLRAVIHVS+LDRSI+
Sbjct: 1   MANNLGPFSIVSSLLFLSLIIGTSLAARNLNDNVLEWVKKDHRHFLRAVIHVSDLDRSIR 60

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYT+ FGMK+LK RNFP+RQYRDALVGFG +++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 61  FYTKGFGMKVLKRRNFPERQYRDALVGFGPQNTHFLLELRQRHDSNNVFIGTEFGHFGIA 120

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKSVEKARANGA+VI KPQ+VN+++ AFVQD DGY FKLIQR   ADPL ++MLHV
Sbjct: 121 TQDVYKSVEKARANGALVIQKPQKVNQTMFAFVQDHDGYKFKLIQRISFADPLVQVMLHV 180

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSK-TTVLQLEKRRNVPRDDG 240
           +DLNR+L FYTKALGMKL ++QNNSTGQ V GTLGYG NQSK TTVLQLEKR+N+PRDDG
Sbjct: 181 EDLNRSLNFYTKALGMKLFEKQNNSTGQIVLGTLGYGINQSKTTTVLQLEKRKNIPRDDG 240

Query: 241 RDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMV 300
           RDGYSM+YIGTDNVNKSAEA K+V+KE+GGNLI+EPVL+ DINVKLT FSDPDNWITIMV
Sbjct: 241 RDGYSMVYIGTDNVNKSAEAAKLVMKELGGNLIIEPVLLSDINVKLTGFSDPDNWITIMV 300

Query: 301 DNKDYQRGTL 310
           DNKDYQRG L
Sbjct: 301 DNKDYQRGRL 310

BLAST of Spg000077 vs. ExPASy TrEMBL
Match: A0A6J1DD93 (lactoylglutathione lyase-like OS=Momordica charantia OX=3673 GN=LOC111019033 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.5e-140
Identity = 246/309 (79.61%), Postives = 273/309 (88.35%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           M N   P SI  SLLFFSLIIGT+LAARNLNEN LEWVKRDHRRFLRAVIHVS+LD S++
Sbjct: 2   MPNNFVP-SIFASLLFFSLIIGTTLAARNLNENVLEWVKRDHRRFLRAVIHVSDLDDSVR 61

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYTQ FGMK++K RNFPDRQYRDALVGFG E++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 62  FYTQGFGMKVVKRRNFPDRQYRDALVGFGPENTHFLLELRQRHDSNNVFIGTEFGHFGIA 121

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKS+EKAR NGAVVIH+PQ+VN +I A VQDRDGY FKLIQR P ADPLCE+ML V
Sbjct: 122 TQDVYKSLEKARVNGAVVIHEPQKVNNTIFALVQDRDGYKFKLIQRPPTADPLCEVMLRV 181

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           +DLNR++ FY KALGMKL KRQNNS GQF  GTLGYG +QSKTTVLQ EKR N+ RDDGR
Sbjct: 182 EDLNRSVNFYAKALGMKLFKRQNNSKGQFASGTLGYGTDQSKTTVLQFEKRNNISRDDGR 241

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSMLYI TDNVNKSAEA KVVIK++GGNLI+EP L+P INVK+T FSDPDNWI IMVD
Sbjct: 242 DGYSMLYISTDNVNKSAEAAKVVIKQLGGNLIIEPGLLPSINVKITGFSDPDNWIMIMVD 301

Query: 301 NKDYQRGTL 310
           NKDY+RGTL
Sbjct: 302 NKDYRRGTL 309

BLAST of Spg000077 vs. ExPASy TrEMBL
Match: A0A0A0L2D4 (Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_3G076020 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.9e-139
Identity = 240/309 (77.67%), Postives = 280/309 (90.61%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MAN L PFSI+ SLLF SLIIGTSLAARNLN+N LEWVK+DHR FLRAVIHVS+LDRSI+
Sbjct: 1   MANNLGPFSIISSLLFLSLIIGTSLAARNLNDNVLEWVKKDHRHFLRAVIHVSDLDRSIR 60

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
           FYT+ FGMK+LK RNFPDRQYRDALVGFG E++HFL+ELRQRHDS++VFIGTEFGHFGIA
Sbjct: 61  FYTKGFGMKVLKRRNFPDRQYRDALVGFGPENTHFLLELRQRHDSNNVFIGTEFGHFGIA 120

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQDVYKSVEKARANGA+VI KPQ++N+++ AFVQD DGY FKLIQ    ADPL ++M HV
Sbjct: 121 TQDVYKSVEKARANGALVIQKPQKINQTMFAFVQDHDGYKFKLIQSKCLADPLVQVMFHV 180

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           QDLNR++ FYTKALGMKL +++NNSTGQ V GTLGYG NQSKTTVLQLEKR+N+PRDDGR
Sbjct: 181 QDLNRSINFYTKALGMKLFEKKNNSTGQIVSGTLGYGINQSKTTVLQLEKRKNIPRDDGR 240

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSM+YIGTDNVNKSA+A K+V+KE+GG++I+EP+L+ +INVKLT F DPDNWITIMVD
Sbjct: 241 DGYSMVYIGTDNVNKSADAAKLVMKELGGSVIIEPILLSNINVKLTGFFDPDNWITIMVD 300

Query: 301 NKDYQRGTL 310
           NKDY++G L
Sbjct: 301 NKDYRKGRL 309

BLAST of Spg000077 vs. ExPASy TrEMBL
Match: A0A5A7SU24 (Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1418G00070 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.5e-135
Identity = 235/290 (81.03%), Postives = 268/290 (92.41%), Query Frame = 0

Query: 21  IGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQ 80
           +GTSLAARNLN+N LEWVK+DHRRFLRAVIHVS+LDRSI+FYT+ FGMK+LK RNFP+RQ
Sbjct: 1   MGTSLAARNLNDNVLEWVKKDHRRFLRAVIHVSDLDRSIRFYTKGFGMKVLKRRNFPERQ 60

Query: 81  YRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIH 140
           YRDALVGFG +++HFL+ELRQRHDS++VFIGTEFGHFGIATQDVYKSVEKARANGA+VI 
Sbjct: 61  YRDALVGFGPQNTHFLLELRQRHDSNNVFIGTEFGHFGIATQDVYKSVEKARANGALVIQ 120

Query: 141 KPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLK 200
           KPQ+VN+++ AFVQD DGY FKLIQR   ADPL ++MLHV+DLNR+L FYTKALGMKL +
Sbjct: 121 KPQKVNQTMFAFVQDHDGYKFKLIQRISFADPLVQVMLHVEDLNRSLNFYTKALGMKLFE 180

Query: 201 RQNNSTGQFVWGTLGYGANQSK-TTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEA 260
           +QNNSTGQ V GTLGYG NQSK TTVLQLEKR+N+PRDDGRDGYSM+YIGTDNVNKSAEA
Sbjct: 181 KQNNSTGQIVLGTLGYGINQSKTTTVLQLEKRKNIPRDDGRDGYSMVYIGTDNVNKSAEA 240

Query: 261 TKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVDNKDYQRGTL 310
            K+V+KE+GGNLI+EPVL+ DINVKLT FSDPDNWITIMVDNKDYQRG L
Sbjct: 241 AKLVMKELGGNLIIEPVLLSDINVKLTGFSDPDNWITIMVDNKDYQRGRL 290

BLAST of Spg000077 vs. ExPASy TrEMBL
Match: A0A6J1F142 (lactoylglutathione lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111438601 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.3e-130
Identity = 233/309 (75.40%), Postives = 265/309 (85.76%), Query Frame = 0

Query: 1   MANKLAPFSILCSLLFFSLIIGTSLAARNLNENALEWVKRDHRRFLRAVIHVSNLDRSIK 60
           MAN L   SIL SLLFFSLII TSL ARNLNEN L+WVK+DHRRFLRAVIHVS+LD SIK
Sbjct: 3   MANNLGLSSILFSLLFFSLIIETSLGARNLNENVLDWVKKDHRRFLRAVIHVSDLDHSIK 62

Query: 61  FYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIA 120
            YT+ FGMKLLK RNFPDR Y+DALVGFG +++HFL+ELRQR   S+VFIGTEFGHFGIA
Sbjct: 63  VYTRGFGMKLLKRRNFPDRGYKDALVGFGPQNTHFLLELRQRDGLSNVFIGTEFGHFGIA 122

Query: 121 TQDVYKSVEKARANGAVVIHKPQRVNKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHV 180
           TQ+VYKSVE+ARANGAVVIH+P++VN++I AFVQD DGY FK IQ   P DPL  IML V
Sbjct: 123 TQNVYKSVEQARANGAVVIHEPEKVNQTIFAFVQDHDGYKFKFIQTPSPLDPLSPIMLRV 182

Query: 181 QDLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGR 240
           Q+L+ +  FY+KALGMKL KRQNNSTG+F+  TLGYG NQSKTT+L+LEKR N+ RDDGR
Sbjct: 183 QNLDLSANFYSKALGMKLFKRQNNSTGEFILATLGYGTNQSKTTLLELEKRNNISRDDGR 242

Query: 241 DGYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVD 300
           DGYSMLYI TDNVNK+AEA KVVIKE+GGNLIMEPVL+P INVK+T F+DPD W  IMVD
Sbjct: 243 DGYSMLYISTDNVNKTAEAAKVVIKELGGNLIMEPVLVPTINVKMTGFTDPDGWRMIMVD 302

Query: 301 NKDYQRGTL 310
           NKDY+RGTL
Sbjct: 303 NKDYRRGTL 311

BLAST of Spg000077 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 259.6 bits (662), Expect = 3.2e-69
Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 0

Query: 8   FSILCSLLFFSLIIGTS--LAARNLNE--NALEWVKRDHRRFLRAVIHVSNLDRSIKFYT 67
           F  +C++ F S+    S  L +RN+ E  + LEW K+D+RRFL  V  V +LDR+I+FYT
Sbjct: 16  FISICNVHFVSMRAAESSFLLSRNMAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYT 75

Query: 68  QAFGMKLLKTRNFPDRQYRDALVGFGSESSHFLVELRQRHDSSDVFIGTEFGHFGIATQD 127
           + FGMKLL+ R+ P+ +Y +A +GFG E+S+F+VEL   +  S   IGT FGHF I+TQD
Sbjct: 76  EVFGMKLLRKRDIPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQD 135

Query: 128 VYKSVEKARANGAVVIHKPQRV--NKSILAFVQDRDGYMFKLIQRSPPADPLCEIMLHVQ 187
           V K VE  RA G  V  +P  V    S++AFV+D DGY F+LIQR P  +P C++ML V 
Sbjct: 136 VSKLVENVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVG 195

Query: 188 DLNRALTFYTKALGMKLLKRQNNSTGQFVWGTLGYGANQSKTTVLQLEKRRNVPRDDGRD 247
           DL+RA+ FY KALGM+LL++      ++  G +GY A + ++ VL+L    +V      +
Sbjct: 196 DLDRAIKFYEKALGMRLLRKIERPEYKYTIGMMGY-AEEYESIVLELTYNYDVTEYTKGN 255

Query: 248 GYSMLYIGTDNVNKSAEATKVVIKEVGGNLIMEPVLIPDINVKLTSFSDPDNWITIMVDN 305
            Y+ + IGTD+V KS E  K+V +E+GG +  E   +P +  K+ SF DPD W T++VDN
Sbjct: 256 AYAQIAIGTDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDN 315

BLAST of Spg000077 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-68
Identity = 128/272 (47.06%), Postives = 180/272 (66.18%), Query Frame = 0

Query: 35  LEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSH 94
           LEW K+D+RRFL  V  V +LDR+I+FYT+ FGMKLL+ R+ P+ +Y +A +GFG E+S+
Sbjct: 8   LEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSN 67

Query: 95  FLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKSILAF 154
           F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S++AF
Sbjct: 68  FVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVIAF 127

Query: 155 VQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWG 214
           V+D DGY F+LIQR P  +P C++ML V DL+RA+ FY KALGM+LL++      ++  G
Sbjct: 128 VKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIG 187

Query: 215 TLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLI 274
            +GY A + ++ VL+L    +V      + Y+ + IGTD+V KS E  K+V +E+GG + 
Sbjct: 188 MMGY-AEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKIT 247

Query: 275 MEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
            E   +P +  K+ SF DPD W T++VDNKD+
Sbjct: 248 REAGPLPGLGTKIVSFLDPDGWKTVLVDNKDF 278

BLAST of Spg000077 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-68
Identity = 128/272 (47.06%), Postives = 180/272 (66.18%), Query Frame = 0

Query: 35  LEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSH 94
           LEW K+D+RRFL  V  V +LDR+I+FYT+ FGMKLL+ R+ P+ +Y +A +GFG E+S+
Sbjct: 8   LEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSN 67

Query: 95  FLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKSILAF 154
           F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S++AF
Sbjct: 68  FVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVIAF 127

Query: 155 VQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWG 214
           V+D DGY F+LIQR P  +P C++ML V DL+RA+ FY KALGM+LL++      ++  G
Sbjct: 128 VKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIG 187

Query: 215 TLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLI 274
            +GY A + ++ VL+L    +V      + Y+ + IGTD+V KS E  K+V +E+GG + 
Sbjct: 188 MMGY-AEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKIT 247

Query: 275 MEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
            E   +P +  K+ SF DPD W T++VDNKD+
Sbjct: 248 REAGPLPGLGTKIVSFLDPDGWKTVLVDNKDF 278

BLAST of Spg000077 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-68
Identity = 128/272 (47.06%), Postives = 180/272 (66.18%), Query Frame = 0

Query: 35  LEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSH 94
           LEW K+D+RRFL  V  V +LDR+I+FYT+ FGMKLL+ R+ P+ +Y +A +GFG E+S+
Sbjct: 8   LEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSN 67

Query: 95  FLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKSILAF 154
           F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S++AF
Sbjct: 68  FVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVIAF 127

Query: 155 VQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWG 214
           V+D DGY F+LIQR P  +P C++ML V DL+RA+ FY KALGM+LL++      ++  G
Sbjct: 128 VKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIG 187

Query: 215 TLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLI 274
            +GY A + ++ VL+L    +V      + Y+ + IGTD+V KS E  K+V +E+GG + 
Sbjct: 188 MMGY-AEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKIT 247

Query: 275 MEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
            E   +P +  K+ SF DPD W T++VDNKD+
Sbjct: 248 REAGPLPGLGTKIVSFLDPDGWKTVLVDNKDF 278

BLAST of Spg000077 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-68
Identity = 128/272 (47.06%), Postives = 180/272 (66.18%), Query Frame = 0

Query: 35  LEWVKRDHRRFLRAVIHVSNLDRSIKFYTQAFGMKLLKTRNFPDRQYRDALVGFGSESSH 94
           LEW K+D+RRFL  V  V +LDR+I+FYT+ FGMKLL+ R+ P+ +Y +A +GFG E+S+
Sbjct: 8   LEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSN 67

Query: 95  FLVELRQRHDSSDVFIGTEFGHFGIATQDVYKSVEKARANGAVVIHKPQRV--NKSILAF 154
           F+VEL   +  S   IGT FGHF I+TQDV K VE  RA G  V  +P  V    S++AF
Sbjct: 68  FVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVIAF 127

Query: 155 VQDRDGYMFKLIQRSPPADPLCEIMLHVQDLNRALTFYTKALGMKLLKRQNNSTGQFVWG 214
           V+D DGY F+LIQR P  +P C++ML V DL+RA+ FY KALGM+LL++      ++  G
Sbjct: 128 VKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIG 187

Query: 215 TLGYGANQSKTTVLQLEKRRNVPRDDGRDGYSMLYIGTDNVNKSAEATKVVIKEVGGNLI 274
            +GY A + ++ VL+L    +V      + Y+ + IGTD+V KS E  K+V +E+GG + 
Sbjct: 188 MMGY-AEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKIT 247

Query: 275 MEPVLIPDINVKLTSFSDPDNWITIMVDNKDY 305
            E   +P +  K+ SF DPD W T++VDNKD+
Sbjct: 248 REAGPLPGLGTKIVSFLDPDGWKTVLVDNKDF 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008463405.11.0e-14180.65PREDICTED: lactoylglutathione lyase-like [Cucumis melo][more]
XP_038903299.14.2e-14078.96lactoylglutathione lyase-like [Benincasa hispida][more]
XP_022151011.19.4e-14079.61lactoylglutathione lyase-like [Momordica charantia][more]
XP_011650522.16.1e-13977.67lactoylglutathione lyase [Cucumis sativus] >KGN56115.1 hypothetical protein Csa_... [more]
KAA0034003.11.5e-13481.03lactoylglutathione lyase-like [Cucumis melo var. makuwa] >TYK02802.1 lactoylglut... [more]
Match NameE-valueIdentityDescription
O653988.4e-6747.06Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1[more]
Q948T69.3e-6644.93Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=... [more]
Q393663.0e-6446.01Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ... [more]
Q8W5932.1e-6243.06Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q555951.3e-1934.38Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803 / Kazusa... [more]
Match NameE-valueIdentityDescription
A0A1S3CJ494.8e-14280.65Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103501576 PE=3 SV=1[more]
A0A6J1DD934.5e-14079.61lactoylglutathione lyase-like OS=Momordica charantia OX=3673 GN=LOC111019033 PE=... [more]
A0A0A0L2D42.9e-13977.67Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_3G076020 PE=3 SV=1[more]
A0A5A7SU247.5e-13581.03Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1418G00070 ... [more]
A0A6J1F1423.3e-13075.40lactoylglutathione lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111438601 PE=4... [more]
Match NameE-valueIdentityDescription
AT1G11840.63.2e-6945.21glyoxalase I homolog [more]
AT1G11840.26.0e-6847.06glyoxalase I homolog [more]
AT1G11840.36.0e-6847.06glyoxalase I homolog [more]
AT1G11840.16.0e-6847.06glyoxalase I homolog [more]
AT1G11840.46.0e-6847.06glyoxalase I homolog [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 309..309
NoneNo IPR availablePANTHERPTHR46036:SF12LACTOYLGLUTATHIONE LYASEcoord: 13..306
NoneNo IPR availablePANTHERPTHR46036LACTOYLGLUTATHIONE LYASEcoord: 13..306
IPR004361Glyoxalase ITIGRFAMTIGR00068TIGR00068coord: 33..174
e-value: 5.5E-38
score: 127.7
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseGENE3D3.10.180.10coord: 19..167
e-value: 1.5E-36
score: 127.7
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseGENE3D3.10.180.10coord: 170..307
e-value: 1.8E-21
score: 78.8
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseSUPERFAMILY54593Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenasecoord: 174..296
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseSUPERFAMILY54593Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenasecoord: 44..172
IPR004360Glyoxalase/fosfomycin resistance/dioxygenase domainPFAMPF00903Glyoxalasecoord: 176..237
e-value: 3.4E-6
score: 27.3
coord: 48..162
e-value: 3.9E-11
score: 43.2
IPR037523Vicinal oxygen chelate (VOC) domainPROSITEPS51819VOCcoord: 172..301
score: 12.512345
IPR037523Vicinal oxygen chelate (VOC) domainPROSITEPS51819VOCcoord: 44..166
score: 21.40167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000077.1Spg000077.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004462 lactoylglutathione lyase activity
molecular_function GO:0046872 metal ion binding