Sgr030562 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr030562 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 1.1e-98
Identity = 189/320 (59.06%), Postives = 248/320 (77.50%), Query Frame = 0

Query: 2   EVIQTSREDTQLSENCNDNERQKSSSKTND-PCAIDIEPRKKGTGTVC---KYEGEADIA 61
           EV +   +D   + N N+N    S  +  D  C +++E  ++  G+     + E EA+  
Sbjct: 75  EVAEIQEQDNNNNNNNNNNNNNISEVENGDVHCVVNVECEEQEKGSASNNGRCECEAENG 134

Query: 62  KEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF- 121
            +E   +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DACF 
Sbjct: 135 TQETSFSTKQMAVLALAIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFE 194

Query: 122 KKSINMVNVVSVIISMAGVAMTTTGKTWARDAPTL---NQKHSFVGDAFALLSAMTDGLY 181
           ++S+++VNVV+V +SMAGVAMTT GKTWARD       ++KHS++GD FALLSA TDGLY
Sbjct: 195 RQSLSIVNVVAVFVSMAGVAMTTVGKTWARDEAQSSFGHEKHSYIGDVFALLSAFTDGLY 254

Query: 182 YVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVI 241
           YVLLKKYAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF LP+S KV E++
Sbjct: 255 YVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIV 314

Query: 242 LADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQ 301
           LA+CF+ NFV+DY WA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYSL+YI GS+Q
Sbjct: 315 LANCFVSNFVSDYLWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQ 374

Query: 302 VFVGFIVANLSDWLTPKLKL 309
           VF+GF++AN SDW++PKLKL
Sbjct: 375 VFLGFMIANFSDWISPKLKL 394

BLAST of Sgr030562 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-98
Identity = 189/329 (57.45%), Postives = 252/329 (76.60%), Query Frame = 0

Query: 5   QTSREDTQLSENCNDNERQKSSSKTND-------------PCAIDIEPRKKGTGTVC--- 64
           + S+E  ++ E  N+N    +++  N+              C ++IE  ++  G+V    
Sbjct: 71  RNSKEVAEIQEQDNNNNNNNTNNNNNNNNNNISEVENGDVHCVVNIECEEQEKGSVSNNG 130

Query: 65  KYEGEADIAKEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLF 124
           + E EA+   +E   +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLF
Sbjct: 131 RCECEAENGTQETPFSTKQMAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLF 190

Query: 125 TLMIDACF-KKSINMVNVVSVIISMAGVAMTTTGKTWARDAPTL---NQKHSFVGDAFAL 184
           TL++DACF ++S+++VNVV+V +SMAGVAMTT GKTWARD       ++KHS++GD FAL
Sbjct: 191 TLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWARDETQSSFGHEKHSYIGDVFAL 250

Query: 185 LSAMTDGLYYVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALP 244
           LSA TDGLYYVLLKKYAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P
Sbjct: 251 LSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAMGIEPKFGIP 310

Query: 245 KSVKVVEVILADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYS 304
           +S KV E++LA+CF+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYS
Sbjct: 311 QSTKVAEIVLANCFVSNFVSDYFWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYS 370

Query: 305 LLYILGSIQVFVGFIVANLSDWLTPKLKL 309
           L+YI GS+QVF+GF++AN SDW++PKLKL
Sbjct: 371 LVYIFGSLQVFLGFMIANFSDWISPKLKL 399

BLAST of Sgr030562 vs. NCBI nr
Match: XP_011648470.2 (uncharacterized transporter C405.03c [Cucumis sativus])

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-98
Identity = 182/260 (70.00%), Postives = 225/260 (86.54%), Query Frame = 0

Query: 59  EERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-K 118
           E+ K +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL+++AC  +
Sbjct: 69  EKAKFSTKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILNACLER 128

Query: 119 KSINMVNVVSVIISMAGVAMTTTGKTWARDAPTLNQ----KHSFVGDAFALLSAMTDGLY 178
           +S+ +VNVV+VI+SMAGVAMTT GKTWARD P  +     KHSFVGDAFALLSA+TDGLY
Sbjct: 129 QSLTIVNVVAVIVSMAGVAMTTVGKTWARDEPQSSSSGHGKHSFVGDAFALLSALTDGLY 188

Query: 179 YVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVI 238
           YVLLK+YAGE  +K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KVVEV+
Sbjct: 189 YVLLKRYAGEEAEKVDMQKFLGYVGLFTLTTLWWLFWPLRAIGIEPKFMIPQSTKVVEVV 248

Query: 239 LADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQ 298
           LA+CF+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYSL+YI GSIQ
Sbjct: 249 LANCFVANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMVGDMVLHGRHYSLVYIFGSIQ 308

Query: 299 VFVGFIVANLSDWLTPKLKL 309
           VF+GFI+ANLSDW++PKLKL
Sbjct: 309 VFLGFIIANLSDWISPKLKL 328

BLAST of Sgr030562 vs. NCBI nr
Match: XP_023007421.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-98
Identity = 186/312 (59.62%), Postives = 248/312 (79.49%), Query Frame = 0

Query: 10  DTQLSENCNDNERQKSSSKTNDP-CAIDIEPRKKGTGTVC---KYEGEADIAKEERKLNT 69
           +  ++ N N+N    S  +  D  C ++IE  ++  G+V    + E EA+   +E   +T
Sbjct: 88  NNNINNNNNNNNNNISEVENGDVLCVVNIECEEQEKGSVSNNDRCECEAENGTQETSFST 147

Query: 70  KEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-KKSINMVN 129
           K++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DACF ++S+++VN
Sbjct: 148 KKIAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVN 207

Query: 130 VVSVIISMAGVAMTTTGKTWARD---APTLNQKHSFVGDAFALLSAMTDGLYYVLLKKYA 189
           VV+V +SMAGVAMTT GKTWARD   + + ++KHS+VGD FALLSA TDGLYYVLLKKYA
Sbjct: 208 VVAVFVSMAGVAMTTIGKTWARDEAQSSSRHEKHSYVGDVFALLSAFTDGLYYVLLKKYA 267

Query: 190 GENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVILADCFLGN 249
           GE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV E+++A+CF+ N
Sbjct: 268 GEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSN 327

Query: 250 FVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFIVA 309
           FV+DY WA+G +WTSPL+AALG+SLTIPLAM+ DM+LHGRHYSL+YI GS+QVF+GF++A
Sbjct: 328 FVSDYLWAMGVVWTSPLIAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIA 387

BLAST of Sgr030562 vs. NCBI nr
Match: TYK06345.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 369.8 bits (948), Expect = 2.4e-98
Identity = 191/314 (60.83%), Postives = 241/314 (76.75%), Query Frame = 0

Query: 5   QTSREDTQLSENCNDNERQKSSSKTNDPCAIDIEPRKKGTGTVCKYEGEADIAKEERKLN 64
           +    D Q   N NDN    ++S  N+    ++   +     + K         EE K +
Sbjct: 105 KVENRDVQYVVNINDNNNNSNNSSNNNG-GCELREAENMEMRILK--------TEEAKFS 164

Query: 65  TKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-KKSINMV 124
           TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DAC  ++S+ +V
Sbjct: 165 TKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIV 224

Query: 125 NVVSVIISMAGVAMTTTGKTWARDAPTLNQ----KHSFVGDAFALLSAMTDGLYYVLLKK 184
           NVV+V++SMAGVAMTT GKTWA+D P  +     KHSFVGDAFALLSA+TDGLYYVLLKK
Sbjct: 225 NVVAVVVSMAGVAMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKK 284

Query: 185 YAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVILADCFL 244
           YAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV EV+LA+CF+
Sbjct: 285 YAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFV 344

Query: 245 GNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFI 304
            NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYSL+YI GSIQVF+GFI
Sbjct: 345 ANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFI 404

Query: 305 VANLSDWLTPKLKL 309
           +AN SDW++PKLKL
Sbjct: 405 IANFSDWISPKLKL 409

BLAST of Sgr030562 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.2e-29
Identity = 92/257 (35.80%), Postives = 136/257 (52.92%), Query Frame = 0

Query: 57  AKEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDA-C 116
           A ++++L   E   L+     LWFT+  +T+ +LA TSVAS TIL +TS  FTL I A C
Sbjct: 115 ANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGAIC 174

Query: 117 FKKSINMVNVVSVIISMAGVAMTTTGKTWAR--------DAPTLNQKHSFVGDAFALLSA 176
             +S++   V+   IS  G+ M T   +  R             +     +G+  AL  A
Sbjct: 175 HVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGA 234

Query: 177 MTDGLYYVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWLAL-----GIEPKFALPKSV 236
           +  G+Y  LLK+  G+   +++++ F GFVGLF    LW   +     G EP F+LPK  
Sbjct: 235 VLYGVYSTLLKREVGDE-TRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGWEP-FSLPKDP 294

Query: 237 KVVEVILADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLY 296
           KVV +I  +C +  FV+D+ WA   + TSPL   +G S+TIPLAM  D+I   +  S LY
Sbjct: 295 KVVVIIFVNCLI-TFVSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALY 354

Query: 297 ILGSIQVFVGFIVANLS 300
           + G+  +   F + N S
Sbjct: 355 LFGATLILGSFFIINKS 368

BLAST of Sgr030562 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 76/237 (32.07%), Postives = 129/237 (54.43%), Query Frame = 0

Query: 66  KEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACFK-KSINMVN 125
           K+   L++    LWF +    + AL++T+VAS+TIL STS  FTL +      ++ +   
Sbjct: 115 KDTMRLSLLFCVLWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKK 174

Query: 126 VVSVIISMAGVAMTTTGKTWARDAPTLNQKHSFVGDAFALLSAMTDGLYYVLLKKYAGEN 185
           ++ + +S+ G+ +     +  +D  +++     VG+  ALL ++   +Y  LLK      
Sbjct: 175 LLGLFVSLFGIILIVMQSSKQQD--SVSASSFLVGNTLALLGSLGYSVYTTLLKYEISSK 234

Query: 186 GQKLDLQKFLGFVGLFAFSGLWWLALGIE----PKFALPKSVKVVEVILADCFLGNFVAD 245
           G +LD+Q FLG+VG+F F   W + + ++      F LP +  +  +++ +C +  FV+D
Sbjct: 235 GLRLDIQMFLGYVGIFTFLLFWPILIILDITHMETFELPSNFHISFLVMLNCII-IFVSD 294

Query: 246 YFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFIVAN 298
           YFW    I TSPLV  +  + TIPLAM AD +     ++  YI+G I +FV F + N
Sbjct: 295 YFWCKALILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVIFIFVSFFLVN 348

BLAST of Sgr030562 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-25
Identity = 79/241 (32.78%), Postives = 132/241 (54.77%), Query Frame = 0

Query: 63  LNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACFK-KSIN 122
           L  ++ A L++    +WF + Y ++ +L  T+VAS TI+ S SG FTL +      +   
Sbjct: 97  LGFRQTAYLSLGFCIIWFAANYFSNSSLGFTNVASFTIISSMSGFFTLGLGTIVNVERFT 156

Query: 123 MVNVVSVIISMAGVAMTTTGKTWARDAPTLNQKHSFVGDAFALLSAMTDGLYYVLLKKYA 182
           +  +++++ S+ GV +  T      D          +G+A+ALL+A+  G Y V++K + 
Sbjct: 157 LSKLLALMASVGGVIIVVTQDAKQADLNDSPPSRPALGNAYALLAALLYGCYSVMVKFHI 216

Query: 183 GENGQKLDLQKFLGFVGLFAFSGLWWLAL-----GIEPKFALPKSVKVVEVILADCFLGN 242
            E    +  + F G VGLF    LW   +     G+E +F+LP +   + V++ +  +  
Sbjct: 217 TEE-SCVSTRLFFGLVGLFDLILLWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-T 276

Query: 243 FVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFIVA 298
           FV+DY W +  + TSPL+  +G SL+IPLA+  D++L G + +   ILGS+ VF GFIV 
Sbjct: 277 FVSDYLWVIAMLMTSPLLVTVGMSLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVV 334

BLAST of Sgr030562 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.9e-22
Identity = 83/255 (32.55%), Postives = 133/255 (52.16%), Query Frame = 0

Query: 53  EADIAKEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMI 112
           +  + K   KL   +VA ++     +WF + +    AL+ T VA   IL STSGLFTL++
Sbjct: 226 QESLLKTVGKLTATQVAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLIL 285

Query: 113 DACFKKS----INMVNVVSVIISMAGVAMTTTGKTWARDAPTLNQKHSFVGDAFALLSAM 172
            A F  +      +  +++VI+S+ GV +     + +  +P  N     +G  ++L+ AM
Sbjct: 286 AAMFPSNSGDRFTLSKLLAVILSIGGVVLVNL--SGSEKSPGRNT----IGSIWSLVGAM 345

Query: 173 TDGLYYVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLW--WLAL---GIEPKFALPKSVK 232
              +Y V++K+   +   KLD+  F GFVGLF    LW  +  L   G E  F  P  V 
Sbjct: 346 LYAVYIVMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV- 405

Query: 233 VVEVILADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYI 292
           V+  I+ +  +G  ++++ W  G   TS L+  L  SLTIPL+++ADM +    +S L+ 
Sbjct: 406 VLMCIVINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFF 465

Query: 293 LGSIQVFVGFIVANL 299
            G+I VF  F +A L
Sbjct: 466 AGAIPVFFSFFIATL 471

BLAST of Sgr030562 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 84/258 (32.56%), Postives = 130/258 (50.39%), Query Frame = 0

Query: 53  EADIAKEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMI 112
           +  I K   KL   +VA ++     +WF +      AL+ T VA   IL STSGLFTL++
Sbjct: 225 QESILKTVGKLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLIL 284

Query: 113 DACFKKS----INMVNVVSVIISMAGVAMTT---TGKTWARDAPTLNQKHSFVGDAFALL 172
            A F  +      +  +++VI+S+ GV +     + K+  RD          +G  ++L 
Sbjct: 285 AAVFPSNSGDRFTLSKLLAVILSIGGVVLVNLSGSEKSAGRDT---------IGSIWSLA 344

Query: 173 SAMTDGLYYVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLW--WLAL---GIEPKFALPK 232
            AM   +Y V++K+   +   KLD+  F GFVGLF    LW  +  L   G E  F  P 
Sbjct: 345 GAMLYAVYIVMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPN 404

Query: 233 SVKVVEVILADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSL 292
            V V+  I+ +  +G  ++++ W  G   TS L+  L  SLTIPL+++ADM +    +S 
Sbjct: 405 KV-VLMCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSW 464

Query: 293 LYILGSIQVFVGFIVANL 299
           L+  G+I VF  F +  L
Sbjct: 465 LFFAGAIPVFFSFFIVTL 470

BLAST of Sgr030562 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 5.2e-99
Identity = 189/320 (59.06%), Postives = 248/320 (77.50%), Query Frame = 0

Query: 2   EVIQTSREDTQLSENCNDNERQKSSSKTND-PCAIDIEPRKKGTGTVC---KYEGEADIA 61
           EV +   +D   + N N+N    S  +  D  C +++E  ++  G+     + E EA+  
Sbjct: 75  EVAEIQEQDNNNNNNNNNNNNNISEVENGDVHCVVNVECEEQEKGSASNNGRCECEAENG 134

Query: 62  KEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF- 121
            +E   +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DACF 
Sbjct: 135 TQETSFSTKQMAVLALAIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFE 194

Query: 122 KKSINMVNVVSVIISMAGVAMTTTGKTWARDAPTL---NQKHSFVGDAFALLSAMTDGLY 181
           ++S+++VNVV+V +SMAGVAMTT GKTWARD       ++KHS++GD FALLSA TDGLY
Sbjct: 195 RQSLSIVNVVAVFVSMAGVAMTTVGKTWARDEAQSSFGHEKHSYIGDVFALLSAFTDGLY 254

Query: 182 YVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVI 241
           YVLLKKYAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF LP+S KV E++
Sbjct: 255 YVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIV 314

Query: 242 LADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQ 301
           LA+CF+ NFV+DY WA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYSL+YI GS+Q
Sbjct: 315 LANCFVSNFVSDYLWAMGVVWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQ 374

Query: 302 VFVGFIVANLSDWLTPKLKL 309
           VF+GF++AN SDW++PKLKL
Sbjct: 375 VFLGFMIANFSDWISPKLKL 394

BLAST of Sgr030562 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 8.8e-99
Identity = 186/312 (59.62%), Postives = 248/312 (79.49%), Query Frame = 0

Query: 10  DTQLSENCNDNERQKSSSKTNDP-CAIDIEPRKKGTGTVC---KYEGEADIAKEERKLNT 69
           +  ++ N N+N    S  +  D  C ++IE  ++  G+V    + E EA+   +E   +T
Sbjct: 88  NNNINNNNNNNNNNISEVENGDVLCVVNIECEEQEKGSVSNNDRCECEAENGTQETSFST 147

Query: 70  KEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-KKSINMVN 129
           K++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DACF ++S+++VN
Sbjct: 148 KKIAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVN 207

Query: 130 VVSVIISMAGVAMTTTGKTWARD---APTLNQKHSFVGDAFALLSAMTDGLYYVLLKKYA 189
           VV+V +SMAGVAMTT GKTWARD   + + ++KHS+VGD FALLSA TDGLYYVLLKKYA
Sbjct: 208 VVAVFVSMAGVAMTTIGKTWARDEAQSSSRHEKHSYVGDVFALLSAFTDGLYYVLLKKYA 267

Query: 190 GENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVILADCFLGN 249
           GE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV E+++A+CF+ N
Sbjct: 268 GEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSN 327

Query: 250 FVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFIVA 309
           FV+DY WA+G +WTSPL+AALG+SLTIPLAM+ DM+LHGRHYSL+YI GS+QVF+GF++A
Sbjct: 328 FVSDYLWAMGVVWTSPLIAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIA 387

BLAST of Sgr030562 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.2e-98
Identity = 191/314 (60.83%), Postives = 241/314 (76.75%), Query Frame = 0

Query: 5   QTSREDTQLSENCNDNERQKSSSKTNDPCAIDIEPRKKGTGTVCKYEGEADIAKEERKLN 64
           +    D Q   N NDN    ++S  N+    ++   +     + K         EE K +
Sbjct: 105 KVENRDVQYVVNINDNNNNSNNSSNNNG-GCELREAENMEMRILK--------TEEAKFS 164

Query: 65  TKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-KKSINMV 124
           TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DAC  ++S+ +V
Sbjct: 165 TKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIV 224

Query: 125 NVVSVIISMAGVAMTTTGKTWARDAPTLNQ----KHSFVGDAFALLSAMTDGLYYVLLKK 184
           NVV+V++SMAGVAMTT GKTWA+D P  +     KHSFVGDAFALLSA+TDGLYYVLLKK
Sbjct: 225 NVVAVVVSMAGVAMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKK 284

Query: 185 YAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVILADCFL 244
           YAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV EV+LA+CF+
Sbjct: 285 YAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFV 344

Query: 245 GNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVGFI 304
            NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM+LHGRHYSL+YI GSIQVF+GFI
Sbjct: 345 ANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFI 404

Query: 305 VANLSDWLTPKLKL 309
           +AN SDW++PKLKL
Sbjct: 405 IANFSDWISPKLKL 409

BLAST of Sgr030562 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.5e-98
Identity = 193/316 (61.08%), Postives = 241/316 (76.27%), Query Frame = 0

Query: 5   QTSREDTQLSENCNDNERQKSSSKTNDPCAIDIEPRKKGTGTVCKYEG-EADIAK-EERK 64
           +    D Q   N NDN    ++S  N+           G   +C+ E  E  I K EE K
Sbjct: 105 EVENRDVQYVVNINDNNNNSNNSSNNN-----------GGCELCEAENMEMRILKTEEAK 164

Query: 65  LNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-KKSIN 124
            +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DAC  ++S+ 
Sbjct: 165 FSTKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLT 224

Query: 125 MVNVVSVIISMAGVAMTTTGKTWARDAPTLNQ----KHSFVGDAFALLSAMTDGLYYVLL 184
           +VN V+V++SMAGV MTT GKTWA+D P  +     KHSFVGDAFALLSA+TDGLYYVLL
Sbjct: 225 IVNAVAVVVSMAGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLL 284

Query: 185 KKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVILADC 244
           KKYAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV EV+LA+C
Sbjct: 285 KKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANC 344

Query: 245 FLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQVFVG 304
           F+ NFV+DYFWA+G +WTSPLVAALG+SLTIPLAM+ DM+LH RHYSL+YI GSIQVF+G
Sbjct: 345 FVANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMIGDMVLHDRHYSLVYIFGSIQVFLG 404

Query: 305 FIVANLSDWLTPKLKL 309
           FI+AN SDW++PKLKL
Sbjct: 405 FIIANFSDWISPKLKL 409

BLAST of Sgr030562 vs. ExPASy TrEMBL
Match: A0A1S3B9F8 (uncharacterized transporter C405.03c-like OS=Cucumis melo OX=3656 GN=LOC103487495 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 4.8e-97
Identity = 178/260 (68.46%), Postives = 221/260 (85.00%), Query Frame = 0

Query: 59  EERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-K 118
           EE K +TK++A+LA+ IGP+WF SEY T+ ALA TSVA+TTILFSTSGLFTL++DAC  +
Sbjct: 85  EEAKFSTKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLER 144

Query: 119 KSINMVNVVSVIISMAGVAMTTTGKTWARDAPTLNQ----KHSFVGDAFALLSAMTDGLY 178
           +S+ +VN V+V++SMAGV MTT GKTWA+D P  +     KHSFVGDAFALLSA+TDGLY
Sbjct: 145 QSLTIVNAVAVVVSMAGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLY 204

Query: 179 YVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFALPKSVKVVEVI 238
           YVLLKKYAGE G+K+D+QKFLG+VGLF  + LWWL     A+GIEPKF +P+S KV EV+
Sbjct: 205 YVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVV 264

Query: 239 LADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQ 298
           LA+CF+ NFV+DYFWA+G +WTSPLVAALG+SL+IPLAM+ DM+LHGRHYSL+YI GSIQ
Sbjct: 265 LANCFVANFVSDYFWAMGVVWTSPLVAALGASLSIPLAMIGDMVLHGRHYSLVYIFGSIQ 324

Query: 299 VFVGFIVANLSDWLTPKLKL 309
           VF+GFI+AN SDW++ KLKL
Sbjct: 325 VFLGFIIANFSDWISLKLKL 344

BLAST of Sgr030562 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 290.8 bits (743), Expect = 1.3e-78
Identity = 149/270 (55.19%), Postives = 198/270 (73.33%), Query Frame = 0

Query: 49  KYEGEADIAKEERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLF 108
           K   E    K+ +++ TK++A+  + + P+WF +EYL++ ALA TSVASTT+L STSGLF
Sbjct: 125 KVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTTVLSSTSGLF 184

Query: 109 TLMIDACF-KKSINMVNVVSVIISMAGVAMTTTGKTWARDA----PTLNQKHSFVGDAFA 168
           TL I     + ++N+  VV+V +SMAGV MTT GKTWA D      +LN + S +GD F 
Sbjct: 185 TLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGERSLMGDLFG 244

Query: 169 LLSAMTDGLYYVLLKKYAGENGQKLDLQKFLGFVGLFAFSGLWWL-----ALGIEPKFAL 228
           LLSA++ GL+ VLLKK+AGE G+ +D+QK  G++GLF    LWWL     ALGIEPKF +
Sbjct: 245 LLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEPKFTI 304

Query: 229 PKSVKVVEVILADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHY 288
           P SVKV EV+LA+ F+G+ ++DYFWAL  +WT+PLVA LG SLTIPLAM+ADM++HGRHY
Sbjct: 305 PHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVADMMIHGRHY 364

Query: 289 SLLYILGSIQVFVGFIVANLSDWLTPKLKL 309
           S +YILGS QVF GF++AN+SD  + KL L
Sbjct: 365 SAIYILGSTQVFAGFVIANISDLFSKKLGL 394

BLAST of Sgr030562 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 90.1 bits (222), Expect = 3.4e-18
Identity = 71/258 (27.52%), Postives = 127/258 (49.22%), Query Frame = 0

Query: 59  EERKLNTKEVALLAICIGPLWFTSEYLTSCALAHTSVASTTILFSTSGLFTLMIDACF-K 118
           E+ +     VA +++ I P WF ++   + +L +T+V S TIL S S LFT ++   F  
Sbjct: 154 EKGRWTRMRVAKVSLVICPFWFLAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLG 213

Query: 119 KSINMVNVVSVIISMAGVAMTTTGKTWARDAPTLNQKHSFVGDAFALLSAMTDGLYYVLL 178
           +    + + SV++ M+G  + + G + ++       K+  +GD  +L+SA    +Y  L+
Sbjct: 214 EKFTWLKLFSVLLCMSGTIIVSMGDSESKSNAV--AKNPLLGDILSLVSAALYAVYITLI 273

Query: 179 KKYA---GENGQKLDLQKFLGFVGLFAFSGLWWLALGIEPKFALPK-----SVKVVEVIL 238
           +K      E   ++ + +FLGF+GLF F    +L   +   F   +     ++K   +++
Sbjct: 274 RKKLPDDDERNGRVSMAQFLGFLGLFNF--FIFLPPALILNFTKRERFNALTLKQFGLVV 333

Query: 239 ADCFLGNFVADYFWALGAIWTSPLVAALGSSLTIPLAMLADMILHGRHYSLLYILGSIQV 298
               L N ++DY WA   + T+  VA  G ++ +PLA + D  L G   S    +G+  V
Sbjct: 334 GKGLLDNVLSDYLWAKAVLLTTTTVATAGLTIQVPLAAIVDS-LSGNKPSFTDYIGAAAV 393

Query: 299 FVGFIVANLSDWLTPKLK 308
            VGF   N+   +  K K
Sbjct: 394 MVGFAGINIPSEMFCKSK 406