Sgr030527 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr030527
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Protein kinase domain-containing protein
Location	tig00154107: 1244938 .. 1247617 (-)
RNA-Seq Expression	Sgr030527
Synteny	Sgr030527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTTGGAGGCACGGGCGCTTCGTGCACCCACCAACGCCCGCCTTGGCTCCACTCTGACTCCATTCCACCGCTGGCTCCCATTAACGAAATTACGCACGTACCCTCAACCTCTCACGACGTCGTTTCGCTTCCAGTGCCTTCGTACGCTTTATTCGGCTCTTTTCCTGCGCTTTGGATACGTTTCTTGAGAGGTCGTTTATACGCTTTTATCGTCGAGAGAAATAAAGAGCTACGCCTTTTTGACCGTATGTCTTCAAAATTGCCCAATCTTTTATCAGAAATAACAAGAATGCCACGCTCTCAACATAGCCACGTATTAAAAAGGGTAAAACTCCGATAAACAACGAAATAAAATATTTATGAGAAACATATTCATAACCAATTTGAATATAGTTTAACTAATTATAATATTATAATTTTTTTTAAATATTAGATATTCGAATTCTCAACCTCATCTCTTGTTTGATCTCAAACAATAATAATAGATATTGTATTATGTGGATTATCTTTTAGTTTTATTTTTAATAAAATTTATAAAGATCAATTAGTGTAGGTATTAGTGTCTTTTTCTATCTGTTGTAAATTTGGATCTAGTTTTCTTGATTTTTGTTTGTAATGTAGTATTATAAAAAAATGTGTAAAGGACCCATAAAAAATTTGAATTTTCCCGTCATAAATATTGTTTTAGTCAAAAAGAGAAAAGATGAATGCTATGAAGAATATAAATTAATGCTGTGTAATTAGCAGGATTGTATTGTTCTTTTTTAAATAAGTCCATGAAATGGCAAAAGCGTGATAGTTGCCCAAAATTGTGGCATTAATAAAGCAGCAAAAAATTAAAATAAAACTACTTTATTTTTTGTAACATATAAGCAAAAGTAACACTTTTTTTTTATATATAAAAAACTTTATTTGCAAAAATATTATATTTGTTGTACTAAAAAGCCCACACAAAGTTCTCTATATAAAAAAGAATACAACAAGGCAAGTAAAACCTCTCTCTCTCTCTCTCTCCTCTCTTTGTCCCTGCCATTCTCCAAGCTTCTGTTCCTGCCATGGCAGCCATAGCCAGAGATGAATCCGACTACGATAGTAGTTGCTCCTCCATAACAGTCCCCGACTCCAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGAAGCCGCCGCCGTAGCAGCTCCGTCTCCATCTACTCCGGCGCAGACGGCGCCTCTCCTCACAAGCCCCACAAGGCCAACCAAGCTGCATGGGAGGCCATGCGGTGGCTCCGGGGAGTCAAAGGCCGCGTTGGGTTGGAACACTTCCGCCTCCTCCGCCGTCTCGGCAGCGGAGATATCGGAAACGTGTATCTCTGTCAGATAAGGAACCCGATGGTGGGTCTGCCGCAGTGCTTCTACGCCATGAAAGTGGTGGACAGAGAAGCTCTGGCCATAAGAAACAAGCTCCGGCGAGCTGAAATGGAGAAGGAGATTCTCACCATGCTCGATCACCCATTTTTGCCGACGCTTTACGCTGAGTTTGATGCTTCTCATTACTCTTGCTTGGTCATGGAGTTTTGCCCCGGCGGCGACTTGTACGCCGCCCGGCAACGGCAGCCAGGAAAACGCTTCTCCATTTCTTCGGCTAAGTAAGCTTTAATGACGTTTTTCTTTTGTGGGAAAAGAAAGTTATGATCAGTGTGGCTAATATTTTAAAAATTTCAATCAAATCTTTTAATTTGTTTCATTAAAGATATTCGTTTAGCCCATAAAGTTTTAAAAGTTGCTAATAGAGGTTTTTTTTAAGAGGATTTTATATAATTTAGCTAATATATAAATAATAACGGAAAAATGGGGTTTTTTTTTGGACAGGTTTTATGCGGCGGAGATAATTTTAGCCCTAGAGTATCTGCATATGATGGGCATTGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTAGAGAAGACGGTCACATAATGCTGTCGGATTTCGATCTCTGCCTGAAATGCGACGTCGTTCCGAAGCTTATCCGGAGCAGAACCACCTCCGACGCCATTGAAAGGCAGGTTAAAAGCTACACCTCGGTAACTTCCTGCGCCGCCGCTCCAATTCAGCCGGTGCTCTCTTGTTTCTCCGCAGCCAGGCGGAAGAGAGCCACGACGGTGACGACGATTACCGAGCACGTCGACGGCGGGAAAGAGGAAGAATTCGACCCGGAGCCGGAGTTGGTGGCGGAGCCGATCAACGCCCGCTCGAAATCCTTCGTCGGAACCCACGAATACCTGGCTCCGGAGGTGATTTCCGGCCAAGGACACGGCAGCGCCGTCGACTGGTGGACGCTAGGTATCTTCCTATACGAAATGTTGTACGGAAAAACCCCATTCAAAGGGGAAAACAACGAGAAAACCCTAATCAGCATCCTGAAGCAGCCATTGAAGTTTCCGAGAATTGGGTTCAGCAGCAGCAGAGAGTATGAAGAGATGGTGAAAGTTGAGGATCTAATCAGCAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATCGGAAGCTTAAAGGGTTCGGTTGAAATCAAAAGACATGAATTTTTCAAAGGGGTCAATTGGGCTCTAATCAGATCCGTCAGACCACCCCAAATCCCTAATTCTGATTTGCATCACAATTTAACAGAAAATACCCAGCTAATTACTTACCCAAATTGA

mRNA sequence

ATGAATTTGGAGGCACGGGCGCTTCGTGCACCCACCAACGCCCGCCTTGGCTCCACTCTGACTCCATTCCACCGCTGGCTCCCATTAACGAAATTACGCACGTACCCTCAACCTCTCACGACGTCGTTTCGCTTCCAGTGCCTTCCTTCTGTTCCTGCCATGGCAGCCATAGCCAGAGATGAATCCGACTACGATAGTAGTTGCTCCTCCATAACAGTCCCCGACTCCAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGAAGCCGCCGCCGTAGCAGCTCCGTCTCCATCTACTCCGGCGCAGACGGCGCCTCTCCTCACAAGCCCCACAAGGCCAACCAAGCTGCATGGGAGGCCATGCGGTGGCTCCGGGGAGTCAAAGGCCGCGTTGGGTTGGAACACTTCCGCCTCCTCCGCCGTCTCGGCAGCGGAGATATCGGAAACGTGTATCTCTGTCAGATAAGGAACCCGATGGTGGGTCTGCCGCAGTGCTTCTACGCCATGAAAGTGGTGGACAGAGAAGCTCTGGCCATAAGAAACAAGCTCCGGCGAGCTGAAATGGAGAAGGAGATTCTCACCATGCTCGATCACCCATTTTTGCCGACGCTTTACGCTGAGTTTGATGCTTCTCATTACTCTTGCTTGGTCATGGAGTTTTGCCCCGGCGGCGACTTGTACGCCGCCCGGCAACGGCAGCCAGGAAAACGCTTCTCCATTTCTTCGGCTAAGTTTTATGCGGCGGAGATAATTTTAGCCCTAGAGTATCTGCATATGATGGGCATTGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTAGAGAAGACGGTCACATAATGCTGTCGGATTTCGATCTCTGCCTGAAATGCGACGTCGTTCCGAAGCTTATCCGGAGCAGAACCACCTCCGACGCCATTGAAAGGCAGGTTAAAAGCTACACCTCGGTAACTTCCTGCGCCGCCGCTCCAATTCAGCCGGTGCTCTCTTGTTTCTCCGCAGCCAGGCGGAAGAGAGCCACGACGGTGACGACGATTACCGAGCACGTCGACGGCGGGAAAGAGGAAGAATTCGACCCGGAGCCGGAGTTGGTGGCGGAGCCGATCAACGCCCGCTCGAAATCCTTCGTCGGAACCCACGAATACCTGGCTCCGGAGGTGATTTCCGGCCAAGGACACGGCAGCGCCGTCGACTGGTGGACGCTAGGTATCTTCCTATACGAAATGTTGTACGGAAAAACCCCATTCAAAGGGGAAAACAACGAGAAAACCCTAATCAGCATCCTGAAGCAGCCATTGAAGTTTCCGAGAATTGGGTTCAGCAGCAGCAGAGAGTATGAAGAGATGGTGAAAGTTGAGGATCTAATCAGCAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATCGGAAGCTTAAAGGGTTCGGTTGAAATCAAAAGACATGAATTTTTCAAAGGGGTCAATTGGGCTCTAATCAGATCCGTCAGACCACCCCAAATCCCTAATTCTGATTTGCATCACAATTTAACAGAAAATACCCAGCTAATTACTTACCCAAATTGA

Coding sequence (CDS)

ATGAATTTGGAGGCACGGGCGCTTCGTGCACCCACCAACGCCCGCCTTGGCTCCACTCTGACTCCATTCCACCGCTGGCTCCCATTAACGAAATTACGCACGTACCCTCAACCTCTCACGACGTCGTTTCGCTTCCAGTGCCTTCCTTCTGTTCCTGCCATGGCAGCCATAGCCAGAGATGAATCCGACTACGATAGTAGTTGCTCCTCCATAACAGTCCCCGACTCCAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGAAGCCGCCGCCGTAGCAGCTCCGTCTCCATCTACTCCGGCGCAGACGGCGCCTCTCCTCACAAGCCCCACAAGGCCAACCAAGCTGCATGGGAGGCCATGCGGTGGCTCCGGGGAGTCAAAGGCCGCGTTGGGTTGGAACACTTCCGCCTCCTCCGCCGTCTCGGCAGCGGAGATATCGGAAACGTGTATCTCTGTCAGATAAGGAACCCGATGGTGGGTCTGCCGCAGTGCTTCTACGCCATGAAAGTGGTGGACAGAGAAGCTCTGGCCATAAGAAACAAGCTCCGGCGAGCTGAAATGGAGAAGGAGATTCTCACCATGCTCGATCACCCATTTTTGCCGACGCTTTACGCTGAGTTTGATGCTTCTCATTACTCTTGCTTGGTCATGGAGTTTTGCCCCGGCGGCGACTTGTACGCCGCCCGGCAACGGCAGCCAGGAAAACGCTTCTCCATTTCTTCGGCTAAGTTTTATGCGGCGGAGATAATTTTAGCCCTAGAGTATCTGCATATGATGGGCATTGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTAGAGAAGACGGTCACATAATGCTGTCGGATTTCGATCTCTGCCTGAAATGCGACGTCGTTCCGAAGCTTATCCGGAGCAGAACCACCTCCGACGCCATTGAAAGGCAGGTTAAAAGCTACACCTCGGTAACTTCCTGCGCCGCCGCTCCAATTCAGCCGGTGCTCTCTTGTTTCTCCGCAGCCAGGCGGAAGAGAGCCACGACGGTGACGACGATTACCGAGCACGTCGACGGCGGGAAAGAGGAAGAATTCGACCCGGAGCCGGAGTTGGTGGCGGAGCCGATCAACGCCCGCTCGAAATCCTTCGTCGGAACCCACGAATACCTGGCTCCGGAGGTGATTTCCGGCCAAGGACACGGCAGCGCCGTCGACTGGTGGACGCTAGGTATCTTCCTATACGAAATGTTGTACGGAAAAACCCCATTCAAAGGGGAAAACAACGAGAAAACCCTAATCAGCATCCTGAAGCAGCCATTGAAGTTTCCGAGAATTGGGTTCAGCAGCAGCAGAGAGTATGAAGAGATGGTGAAAGTTGAGGATCTAATCAGCAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATCGGAAGCTTAAAGGGTTCGGTTGAAATCAAAAGACATGAATTTTTCAAAGGGGTCAATTGGGCTCTAATCAGATCCGTCAGACCACCCCAAATCCCTAATTCTGATTTGCATCACAATTTAACAGAAAATACCCAGCTAATTACTTACCCAAATTGA

Protein sequence

MNLEARALRAPTNARLGSTLTPFHRWLPLTKLRTYPQPLTTSFRFQCLPSVPAMAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASPHKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKLIRSRTTSDAIERQVKSYTSVTSCAAAPIQPVLSCFSAARRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQLITYPN

Homology

[Close]

BLAST of Sgr030527 vs. NCBI nr
Match: XP_022946203.1 (protein kinase PINOID 2-like [Cucurbita moschata])

HSP 1 Score: 760.0 bits (1961), Expect = 1.3e-215
Identity = 393/476 (82.56%), Postives = 420/476 (88.24%), Query Frame = 0


Query: 45  FQCLPSVPAMAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGA 104
           F+  P   AMAAIARDESDYDSSCSSIT+PDS+RSWMSNLSFGS   SSS    S AD  
Sbjct: 21  FEYFPFRSAMAAIARDESDYDSSCSSITLPDSTRSWMSNLSFGS---SSS----SAADAT 80

Query: 105 SP-HKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGL 164
           SP  KPHKAN+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   +
Sbjct: 81  SPVQKPHKANEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARV 140

Query: 165 PQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCP 224
            QC YAMKVVDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCP
Sbjct: 141 GQCCYAMKVVDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCP 200

Query: 225 GGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIML 284
           GGDLYAAR RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIML
Sbjct: 201 GGDLYAARLRQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIML 260

Query: 285 SDFDLCLKCDVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKR 344
           SDFDLCLKCDVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS +RR++
Sbjct: 261 SDFDLCLKCDVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSISRRRK 320

Query: 345 ATTVTTITEHV-DGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD 404
            TTVTTITEH  D G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD
Sbjct: 321 PTTVTTITEHADDDGYNEEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD 380

Query: 405 WWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISK 464
           WWT+GIFLYEM+YGKTPFKGENNEKTL++IL+QPL+FPRIGFSSS+EYEEMVKVEDLI K
Sbjct: 381 WWTVGIFLYEMVYGKTPFKGENNEKTLMNILRQPLRFPRIGFSSSKEYEEMVKVEDLIKK 440

Query: 465 LLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           LLVKNPKKRIGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD H NL++N +L
Sbjct: 441 LLVKNPKKRIGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSD-HLNLSKNPKL 488

[Close]

BLAST of Sgr030527 vs. NCBI nr
Match: XP_022999369.1 (protein kinase PINOID 2-like [Cucurbita maxima])

HSP 1 Score: 759.6 bits (1960), Expect = 1.8e-215
Identity = 389/467 (83.30%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASP-HKPHKA 113
           MAAIA+DESDYDSSCSSIT+PDS+RSWMSNLSFGS+RR SS    S  DG SP  KPHKA
Sbjct: 1   MAAIAKDESDYDSSCSSITLPDSTRSWMSNLSFGSQRRRSS---SSATDGTSPVQKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGLPQCFYAMKV 173
           N+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   + QC YAMKV
Sbjct: 61  NEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARVGQCCYAMKV 120

Query: 174 VDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQ 233
           VDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCPGGDLYAAR 
Sbjct: 121 VDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCPGGDLYAARL 180

Query: 234 RQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKC 293
           RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIMLSDFDLCLKC
Sbjct: 181 RQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIMLSDFDLCLKC 240

Query: 294 DVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKRATTVTTITE 353
           DVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS ARR++ TTVTTITE
Sbjct: 241 DVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSIARRRKPTTVTTITE 300

Query: 354 HV-DGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 413
           H  D G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT+GIFLY
Sbjct: 301 HADDDGYREEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTVGIFLY 360

Query: 414 EMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKR 473
           EMLYGKTPFKGENNEKTL++IL+QPL FPRIGFSSS+EYEEMVKVEDLI KLLVKNPKKR
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILRQPLTFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 420

Query: 474 IGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           IGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD  +  ++N +L
Sbjct: 421 IGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSDHLNPRSKNPKL 464

[Close]

BLAST of Sgr030527 vs. NCBI nr
Match: XP_022965690.1 (protein kinase PINOID 2-like [Cucurbita maxima])

HSP 1 Score: 758.4 bits (1957), Expect = 3.9e-215
Identity = 380/467 (81.37%), Postives = 416/467 (89.08%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGA-SPHKPHKA 113
           MA I RD+SD+DS+CSS+T+PDSSRSWMSNLSFGSRR S+     S  D A SP+KPHKA
Sbjct: 1   MAGITRDDSDHDSTCSSLTLPDSSRSWMSNLSFGSRRSST-----SALDAALSPYKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVD 173
           NQ AWEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLC+IRNP+VGL QC+YAMKVVD
Sbjct: 61  NQVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVD 120

Query: 174 REALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQ 233
           REA+++RNK RRA+MEKEIL ++DHPFLPTLYA+F+ASHYSCLVMEFCPGGDLYAARQR 
Sbjct: 121 REAVSVRNKGRRADMEKEILKLIDHPFLPTLYAQFEASHYSCLVMEFCPGGDLYAARQRL 180

Query: 234 PGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 293
           PGKRF I S KFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV
Sbjct: 181 PGKRFPIPSVKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 240

Query: 294 VPKLIRSRTTSDAIERQVKSYTSVTSCAAAPIQPVLSCFSAARRKRATTVTTITEH---- 353
           VPKLIR RTT++    +  S +S TSCAAAPIQPVLSCFS  RR+ +TT+TTITEH    
Sbjct: 241 VPKLIRCRTTAERHVTKTYSTSSTTSCAAAPIQPVLSCFSITRRRNSTTITTITEHAAES 300

Query: 354 ---VDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 413
              VDG    EFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL
Sbjct: 301 AATVDG----EFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 360

Query: 414 YEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKK 473
           YEMLYGKTPFKGENNEKTL++ILKQPL FPRIG SS++EYEEMVKVEDLI KLLVKNPKK
Sbjct: 361 YEMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 474 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSD--LHHNLTEN 511
           RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSD  LH  LT++
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQS 458

[Close]

BLAST of Sgr030527 vs. NCBI nr
Match: KAG7030399.1 (Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 757.3 bits (1954), Expect = 8.7e-215
Identity = 388/467 (83.08%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASP-HKPHKA 113
           MAAIARDESDYDSSCSSIT+PDS+RSWMSNLSFGS   SS+      AD  SP  KPHKA
Sbjct: 1   MAAIARDESDYDSSCSSITLPDSTRSWMSNLSFGSSSSSSA------ADATSPVQKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGLPQCFYAMKV 173
           N+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   + QC YAMKV
Sbjct: 61  NEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARVGQCCYAMKV 120

Query: 174 VDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQ 233
           VDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCPGGDLYAAR 
Sbjct: 121 VDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCPGGDLYAARL 180

Query: 234 RQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKC 293
           RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIMLSDFDLCLKC
Sbjct: 181 RQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIMLSDFDLCLKC 240

Query: 294 DVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKRATTVTTITE 353
           DVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS +RR++ TTVTTITE
Sbjct: 241 DVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSISRRRKPTTVTTITE 300

Query: 354 HV-DGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 413
           H  D G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT+GIFLY
Sbjct: 301 HADDDGYNEEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTVGIFLY 360

Query: 414 EMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKR 473
           EM+YGKTPFKGENNEKTL++IL+QPL+FPRIGFSSS+EYEEMVKVEDLI KLLVKNPKKR
Sbjct: 361 EMVYGKTPFKGENNEKTLMNILRQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 420

Query: 474 IGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           IGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD H NL++N +L
Sbjct: 421 IGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSD-HLNLSKNPKL 460

[Close]

BLAST of Sgr030527 vs. NCBI nr
Match: KAG6599411.1 (Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 757.3 bits (1954), Expect = 8.7e-215
Identity = 388/467 (83.08%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASP-HKPHKA 113
           MAAIARDESDYDSSCSSIT+PDS+RSWMSNLSFGS   SS+      AD  SP  KPHKA
Sbjct: 1   MAAIARDESDYDSSCSSITLPDSTRSWMSNLSFGSSSSSSA------ADATSPVQKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGLPQCFYAMKV 173
           N+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   + QC YAMKV
Sbjct: 61  NEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARVGQCCYAMKV 120

Query: 174 VDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQ 233
           VDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCPGGDLYAAR 
Sbjct: 121 VDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCPGGDLYAARL 180

Query: 234 RQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKC 293
           RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIMLSDFDLCLKC
Sbjct: 181 RQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIMLSDFDLCLKC 240

Query: 294 DVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKRATTVTTITE 353
           DVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS +RR++ TTVTTITE
Sbjct: 241 DVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSISRRRKPTTVTTITE 300

Query: 354 HVD-GGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 413
           H D  G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT+GIFLY
Sbjct: 301 HADYDGYNEEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTVGIFLY 360

Query: 414 EMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKR 473
           EM+YGKTPFKGENNEKTL++IL+QPL+FPRIGFSSS+EYEEMVKVEDLI KLLVKNPKKR
Sbjct: 361 EMVYGKTPFKGENNEKTLMNILRQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 420

Query: 474 IGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           IGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD H NL++N +L
Sbjct: 421 IGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSD-HLNLSKNPKL 460

[Close]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 6.0e-158
Identity = 294/466 (63.09%), Postives = 354/466 (75.97%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASP--HKPHK 113
           MAAI ++ESDYDSS SS+T PDS RSW+S++   S   S S   + G   AS   +KPHK
Sbjct: 1   MAAI-KEESDYDSSRSSLTAPDSRRSWISDIGSSS---SVSARSFGGDTPASSCRYKPHK 60

Query: 114 ANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQ------CF 173
           ANQA WEA+R LR   GRVGLEHFRL+RRLGSGD+GNVYLC++R P            C 
Sbjct: 61  ANQAEWEAIRRLRAGAGRVGLEHFRLVRRLGSGDLGNVYLCRLREPWSSSSMTTTAGGCL 120

Query: 174 YAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDL 233
           YAMKVVD++ALA R KLRRAE+E++IL  LDHPFLPTLYA+F+ASHY+CLVMEFCPGGDL
Sbjct: 121 YAMKVVDKDALAFRKKLRRAEVERDILRTLDHPFLPTLYADFEASHYACLVMEFCPGGDL 180

Query: 234 YAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFD 293
           + ARQRQPG+RF++SS +FY AE +LALEYLHMMG+VYRDLKPENVLVR DGHIMLSDFD
Sbjct: 181 HVARQRQPGRRFTVSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLVRGDGHIMLSDFD 240

Query: 294 LCLKCDVVPKLIRSRTTSDAIERQVKSYTSVTSCAAAPIQPVLSCFSAA------RRKRA 353
           L LKCDVVPKL+R   ++ A  +        +SC    IQPVLSC           ++ A
Sbjct: 241 LSLKCDVVPKLLRPARSAAAGGK--PPLPPPSSCVPPTIQPVLSCIFRGVHKCHHAKECA 300

Query: 354 TTVTTITEHVDG-GKEEEFD---PEPE-LVAEPINARSKSFVGTHEYLAPEVISGQGHGS 413
                   + DG G +EE +    EPE +V EP+ ARSKSFVGTHEYLAPEVISGQGHGS
Sbjct: 301 GGGAAAGNNGDGDGNDEEAETETAEPEVVVVEPVAARSKSFVGTHEYLAPEVISGQGHGS 360

Query: 414 AVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRI-GFSSSREYEEMVKVED 473
           AVDWWTLG+F+YEMLYG+TPFKGE+NEKTLI+I+KQP+ FPR+ G +++ E+EEM   +D
Sbjct: 361 AVDWWTLGVFMYEMLYGRTPFKGESNEKTLINIIKQPVTFPRLAGAAAAGEWEEMKTAQD 420

Query: 474 LISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 500
           L+ +LL KNPKKR+GS  GS E+KRH FFKGVNWAL+RSVRPP++P
Sbjct: 421 LMLQLLAKNPKKRLGSTMGSAEVKRHPFFKGVNWALVRSVRPPEVP 460

[Close]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot
Match: Q64FQ2 (Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 296/497 (59.56%), Postives = 355/497 (71.43%), Query Frame = 0


Query: 60  DESDYDSSCSSITVPDSSR--SWMSNLSFGSRRRSSSVSIYS--GADGASPHKPHKANQA 119
           +ESDY+SS      PDSSR  SW+S+ SF +    SS+S  S  G +  +  KPHKANQ 
Sbjct: 11  NESDYESSTVG---PDSSRRTSWLSS-SFTASPSCSSISHLSNHGLNSYNQSKPHKANQV 70

Query: 120 AWEAMRWLRGVKGR-VGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQ-CFYAMKVVDR 179
           AWEAM  LR   GR VGLEHFRLL+RLGSGDIG+VYLCQIR    G P+  FYAMKVVD+
Sbjct: 71  AWEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIR----GSPETAFYAMKVVDK 130

Query: 180 EALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQP 239
           EA+A++ KL RAEMEK+IL MLDHPF PTLYA F+ASHYS LVME+CPGGDLYA R RQP
Sbjct: 131 EAVAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCPGGDLYAVRLRQP 190

Query: 240 GKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVV 299
            KRF+ISS +FYAAE ++ALEYLHMMGIVYRDLKPENVL+REDGH+MLSDFDL  KCDVV
Sbjct: 191 SKRFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVMLSDFDLSFKCDVV 250

Query: 300 PKLIRSRT-----TSDAIERQVKSYTSVTSCAAAPIQPVLSCF---SAARRKRATTVTTI 359
           P+ +           D  +  ++   S  SC   P+ PV+SCF   S+ RRK+    TTI
Sbjct: 251 PQFLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSSRRRKKNVVTTTI 310

Query: 360 TEHVDGGKEEEFDPE--------------------------PELVAEPINARSKSFVGTH 419
            E+  G  +     +                          P + AEPINARSKSFVGTH
Sbjct: 311 HENAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGGCPSIFAEPINARSKSFVGTH 370

Query: 420 EYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGF 479
           EYLAPEVISGQGHGSAVDWWT GIFLYEM++G+TPFKG+NNEKTL++ILK PL FP++  
Sbjct: 371 EYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNILKAPLTFPKVIV 430

Query: 480 SSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 517
           +S +EYE+MV  +DLI KLLVKNPKKR+GSLKGS+EIKRHEFF+GVNWALIRS++PP +P
Sbjct: 431 NSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNWALIRSIKPPWVP 490

[Close]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.4e-119
Identity = 218/409 (53.30%), Postives = 281/409 (68.70%), Query Frame = 0


Query: 93  SSVSIYSGADGASPHKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLC 152
           S+ S +SG      +KPHKAN   WEA++ +R   G +GL HFRLL+RLG GDIG V+L 
Sbjct: 86  STCSSFSG-----NNKPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLA 145

Query: 153 QIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHY 212
           ++        +CF+AMKV+D+ ALA R KL RA+ E+EIL  LDHPFLPTLY+ F+   +
Sbjct: 146 ELHG-----TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKF 205

Query: 213 SCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVL 272
           SCLVMEFCPGGDL+  RQRQPGKRFS  +AKFY AE++LA+EYLHM+GI+YRDLKPENVL
Sbjct: 206 SCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVL 265

Query: 273 VREDGHIMLSDFDLCLKCDVVPKLIRSRTTSDAIERQVKSYTSVTSCAAAP--IQPVLSC 332
           VR+DGH+MLSDFDL L+C V P ++RS   +   ++    Y +  +C   P  I    +C
Sbjct: 266 VRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKN-SGYCAQPACIQQPSCISAPTTC 325

Query: 333 FSAARRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQ 392
           FS    +  ++ +   + +      +  P PELVAEP +ARS SFVGTHEYLAPE+I G+
Sbjct: 326 FSP---RYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGE 385

Query: 393 GHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVK 452
           GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL +++ QPL+FP     S         
Sbjct: 386 GHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FA 445

Query: 453 VEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 500
             DLI  LLVK P+ R+   +G+ E+K+H FF+GVNWAL+R   PP+IP
Sbjct: 446 ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP 473

[Close]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.7e-118
Identity = 217/405 (53.58%), Postives = 274/405 (67.65%), Query Frame = 0


Query: 104 ASPHKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQ 163
           +S +KPHKAN   WEA++ +R   G +GL HFRLL+RLG GDIG V+L ++        +
Sbjct: 78  SSINKPHKANDVRWEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAELNG-----TR 137

Query: 164 CFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGG 223
           C++AMKV+D+ ALA R KL RA+ E+EIL  LDHPFLPTLY+ F+   +SCLVMEFCPGG
Sbjct: 138 CYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGG 197

Query: 224 DLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSD 283
           DL+  RQRQPGKRF+  +AKFY AE++LA+EYLHM+GI+YRDLKPENVLVR+DGH+MLSD
Sbjct: 198 DLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSD 257

Query: 284 FDLCLKCDVVPKLIRSRTT-SDAIERQVKSYTSVTSCAAAP---IQPVLSC-----FSAA 343
           FDL L+C V   ++RS    S+ + +   S +   +C   P        SC     FS+ 
Sbjct: 258 FDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCFGPRFFSSK 317

Query: 344 RRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGS 403
            +K     T    H       +  P PELVAEP  ARS SFVGTHEYLAPE+I G+GHGS
Sbjct: 318 SKKDKKPKTENGNH-------QVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHGS 377

Query: 404 AVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDL 463
           AVDWWT GIFLYE+L+GKTPFKG  N  TL +++ QPL+FP     S           DL
Sbjct: 378 AVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAARDL 437

Query: 464 ISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 500
           I  LLVK P+ R+   +G+ EIK+H FF+GVNWAL+R   PP+IP
Sbjct: 438 IRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIP 463

[Close]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot
Match: Q39183 (Serine/threonine-protein kinase D6PKL2 OS=Arabidopsis thaliana OX=3702 GN=D6PKL2 PE=1 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 6.7e-117
Identity = 222/429 (51.75%), Postives = 282/429 (65.73%), Query Frame = 0


Query: 75  DSSRSWMSNLSFGSRRRSSSVSIYSGADGASPHKPHKANQAAWEAMRWLRGVKGRVGLEH 134
           D   S  S++  GS     S      +  ++ +KPHKAN   WEA++ +R   G +GL H
Sbjct: 131 DFMESGKSSICRGSTSSDVSDESSCSSFSSTVNKPHKANDLRWEAIQAVRVRDGLLGLSH 190

Query: 135 FRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTM 194
           FRLL+RLG GDIG+VYL ++        +C++AMKV+D+ +LA R KL RA+ E+EIL  
Sbjct: 191 FRLLKRLGCGDIGSVYLSELSG-----TKCYFAMKVMDKTSLASRKKLLRAQTEREILQS 250

Query: 195 LDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALE 254
           LDHPFLPTLY  F+   +SCLVMEFCPGGDL+  RQRQPGK FS  + KFY AE +LALE
Sbjct: 251 LDHPFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALE 310

Query: 255 YLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKLIRSRT-TSDAIERQVKSY 314
           YLHM+GIVYRDLKPENVLVREDGHIMLSDFDL L+C V P L++S    SD + + V   
Sbjct: 311 YLHMLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSAAIESDPLRKNVYCV 370

Query: 315 TSV---TSCAAAPIQPVLSCFSAARRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINA 374
                  SC         +CFS    +  ++ +            +  P PELVAEP +A
Sbjct: 371 QPACIEPSCIQPSCTVPTTCFSP---RLFSSKSKKDRKPKNDTANQVRPLPELVAEPTDA 430

Query: 375 RSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQ 434
           RS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+G+TPFKG  N +TL +++ Q
Sbjct: 431 RSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNVVGQ 490

Query: 435 PLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALI 494
           PL+FP     S           DLI  LL+K P++R+G  +G+ E+K+H FF+GVNWALI
Sbjct: 491 PLRFPETPVVS-------FAARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVNWALI 544

Query: 495 RSVRPPQIP 500
           R   PP+IP
Sbjct: 551 RCATPPEIP 544

[Close]

BLAST of Sgr030527 vs. ExPASy TrEMBL
Match: A0A6J1G352 (protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450358 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 6.5e-216
Identity = 393/476 (82.56%), Postives = 420/476 (88.24%), Query Frame = 0


Query: 45  FQCLPSVPAMAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGA 104
           F+  P   AMAAIARDESDYDSSCSSIT+PDS+RSWMSNLSFGS   SSS    S AD  
Sbjct: 21  FEYFPFRSAMAAIARDESDYDSSCSSITLPDSTRSWMSNLSFGS---SSS----SAADAT 80

Query: 105 SP-HKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGL 164
           SP  KPHKAN+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   +
Sbjct: 81  SPVQKPHKANEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARV 140

Query: 165 PQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCP 224
            QC YAMKVVDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCP
Sbjct: 141 GQCCYAMKVVDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCP 200

Query: 225 GGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIML 284
           GGDLYAAR RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIML
Sbjct: 201 GGDLYAARLRQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIML 260

Query: 285 SDFDLCLKCDVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKR 344
           SDFDLCLKCDVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS +RR++
Sbjct: 261 SDFDLCLKCDVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSISRRRK 320

Query: 345 ATTVTTITEHV-DGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD 404
            TTVTTITEH  D G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD
Sbjct: 321 PTTVTTITEHADDDGYNEEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVD 380

Query: 405 WWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISK 464
           WWT+GIFLYEM+YGKTPFKGENNEKTL++IL+QPL+FPRIGFSSS+EYEEMVKVEDLI K
Sbjct: 381 WWTVGIFLYEMVYGKTPFKGENNEKTLMNILRQPLRFPRIGFSSSKEYEEMVKVEDLIKK 440

Query: 465 LLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           LLVKNPKKRIGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD H NL++N +L
Sbjct: 441 LLVKNPKKRIGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSD-HLNLSKNPKL 488

[Close]

BLAST of Sgr030527 vs. ExPASy TrEMBL
Match: A0A6J1KGU7 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 8.5e-216
Identity = 389/467 (83.30%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASP-HKPHKA 113
           MAAIA+DESDYDSSCSSIT+PDS+RSWMSNLSFGS+RR SS    S  DG SP  KPHKA
Sbjct: 1   MAAIAKDESDYDSSCSSITLPDSTRSWMSNLSFGSQRRRSS---SSATDGTSPVQKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIR--NPMVGLPQCFYAMKV 173
           N+ AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLCQIR   P   + QC YAMKV
Sbjct: 61  NEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARVGQCCYAMKV 120

Query: 174 VDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQ 233
           VDREA+ +RNK RRAEMEKEIL M+DHPFLPTLYA F+ASHYSCLVMEFCPGGDLYAAR 
Sbjct: 121 VDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCPGGDLYAARL 180

Query: 234 RQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKC 293
           RQP KRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVR DGHIMLSDFDLCLKC
Sbjct: 181 RQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIMLSDFDLCLKC 240

Query: 294 DVVPKLIRSRTT-SDAIERQVKSY--TSVTSCAAAPIQPVLSCFSAARRKRATTVTTITE 353
           DVVPKLIRSRTT SD  ER V+SY  +S TSCAA PI+PVLSCFS ARR++ TTVTTITE
Sbjct: 241 DVVPKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSIARRRKPTTVTTITE 300

Query: 354 HV-DGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 413
           H  D G  EEFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT+GIFLY
Sbjct: 301 HADDDGYREEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTVGIFLY 360

Query: 414 EMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKR 473
           EMLYGKTPFKGENNEKTL++IL+QPL FPRIGFSSS+EYEEMVKVEDLI KLLVKNPKKR
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILRQPLTFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 420

Query: 474 IGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQL 514
           IGSLKGS+EIKRHEFFKGVNWALIRSVRPP IPNSD  +  ++N +L
Sbjct: 421 IGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSDHLNPRSKNPKL 464

[Close]

BLAST of Sgr030527 vs. ExPASy TrEMBL
Match: A0A6J1HPF1 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.9e-215
Identity = 380/467 (81.37%), Postives = 416/467 (89.08%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGA-SPHKPHKA 113
           MA I RD+SD+DS+CSS+T+PDSSRSWMSNLSFGSRR S+     S  D A SP+KPHKA
Sbjct: 1   MAGITRDDSDHDSTCSSLTLPDSSRSWMSNLSFGSRRSST-----SALDAALSPYKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVD 173
           NQ AWEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLC+IRNP+VGL QC+YAMKVVD
Sbjct: 61  NQVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVD 120

Query: 174 REALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQ 233
           REA+++RNK RRA+MEKEIL ++DHPFLPTLYA+F+ASHYSCLVMEFCPGGDLYAARQR 
Sbjct: 121 REAVSVRNKGRRADMEKEILKLIDHPFLPTLYAQFEASHYSCLVMEFCPGGDLYAARQRL 180

Query: 234 PGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 293
           PGKRF I S KFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV
Sbjct: 181 PGKRFPIPSVKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 240

Query: 294 VPKLIRSRTTSDAIERQVKSYTSVTSCAAAPIQPVLSCFSAARRKRATTVTTITEH---- 353
           VPKLIR RTT++    +  S +S TSCAAAPIQPVLSCFS  RR+ +TT+TTITEH    
Sbjct: 241 VPKLIRCRTTAERHVTKTYSTSSTTSCAAAPIQPVLSCFSITRRRNSTTITTITEHAAES 300

Query: 354 ---VDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 413
              VDG    EFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL
Sbjct: 301 AATVDG----EFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 360

Query: 414 YEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKK 473
           YEMLYGKTPFKGENNEKTL++ILKQPL FPRIG SS++EYEEMVKVEDLI KLLVKNPKK
Sbjct: 361 YEMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 474 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSD--LHHNLTEN 511
           RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSD  LH  LT++
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQS 458

[Close]

BLAST of Sgr030527 vs. ExPASy TrEMBL
Match: A0A6J1FGS3 (protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 6.3e-211
Identity = 374/467 (80.09%), Postives = 412/467 (88.22%), Query Frame = 0


Query: 54  MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGA-SPHKPHKA 113
           MA+I RD+SD+DS+ SS+T+ DS+RSW+SNLS  SRR S+     S  D A SP+KPHKA
Sbjct: 1   MASITRDDSDHDSTSSSLTLQDSTRSWLSNLSISSRRSST-----SALDAALSPYKPHKA 60

Query: 114 NQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVD 173
           N  AWEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLC+IRNP+VGL QC+YAMKVVD
Sbjct: 61  NHVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVD 120

Query: 174 REALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQ 233
           REA+++RNK RRA+MEKEIL ++DHPFLPTLYAEF+ASHYSCLVMEFCPGGDLYAARQR 
Sbjct: 121 REAVSVRNKGRRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRL 180

Query: 234 PGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 293
           PGKRF ISS+KFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV
Sbjct: 181 PGKRFPISSSKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDV 240

Query: 294 VPKLIRSRTTSDAIERQVKSYTSVTSCAAAPIQPVLSCFSAARRKRATTVTTITEH---- 353
           VPKLIR RTT++    +  S +S TSCAAAPI PVLSCFS  RR  +TT+TTITEH    
Sbjct: 241 VPKLIRCRTTAERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAES 300

Query: 354 ---VDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 413
              VDG    EFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL
Sbjct: 301 AATVDG----EFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFL 360

Query: 414 YEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKK 473
           YEMLYGKTPFKGENNEKTL++ILKQPL FPRIG SS++EYEEMVKVEDLI KLLVKNPKK
Sbjct: 361 YEMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 474 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSD--LHHNLTEN 511
           RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSD  LH  LT++
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQS 458

[Close]

BLAST of Sgr030527 vs. ExPASy TrEMBL
Match: A0A5D3BGM8 (Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00110 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.5e-209
Identity = 381/486 (78.40%), Postives = 411/486 (84.57%), Query Frame = 0


Query: 56  AIARDE-SDYDSSC-SSITVPDSSRSWMSNLSFGSRRRSSSVSIYSGADGASPH------ 115
           +I RDE SDYDSSC SSITVPDS+RSWMSNLSFGSRRRSSS    S ADG          
Sbjct: 4   SIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSS----SAADGGGLENITSGT 63

Query: 116 -------KPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVG 175
                  KPHKAN+  WEAMRWLR  KG VGLE FRL+RR+GSGDIGNVYLC+IRNPMVG
Sbjct: 64  TTTTMMVKPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRNPMVG 123

Query: 176 LPQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFC 235
           LP+ FYAMKVVDREA+ +RNK RRA+MEKEIL +LDHPFLPTLYA F+ASHYSCLVMEFC
Sbjct: 124 LPKWFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLVMEFC 183

Query: 236 PGGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIM 295
           PGGDLYAAR R P KRFSI SAKFYAAEIILALEYLHMMGI+YRDLKPENVLVREDGHIM
Sbjct: 184 PGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVREDGHIM 243

Query: 296 LSDFDLCLKCDVVPKLIRSRTTSDAIERQVKSYTSVT--SCAAAPIQPVLSCFSAARRKR 355
           LSDFDLCLKCDVVPKL+RSRTT+     +  SYT  T  SCAAAPIQPVLSCFS +RR++
Sbjct: 244 LSDFDLCLKCDVVPKLLRSRTTASDRHLRSSSYTFSTSSSCAAAPIQPVLSCFSLSRRQK 303

Query: 356 ATTVTTITEHVD------GGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGH 415
           +TTVTTITEH        G   EEFDPEPEL+AEPINARSKSFVGTHEYLAPEVISGQGH
Sbjct: 304 STTVTTITEHASDSIYGPGDDGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGH 363

Query: 416 GSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVE 475
           GS VDWWTLGIFLYEMLYGKTPFKGENNEKTL++ILKQPLKFPRIG SSS+EYEEMVKVE
Sbjct: 364 GSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGISSSKEYEEMVKVE 423

Query: 476 DLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDLHHNLTENTQ 519
           DLI KLLVKNPKKRIGSLKGSVEIKRHEFFKG+NWALIRSVRPPQIPN+++ +N   N  
Sbjct: 424 DLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPNNNIINN---NLN 482

[Close]

BLAST of Sgr030527 vs. TAIR 10
Match: AT2G26700.1 (AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein )

HSP 1 Score: 554.7 bits (1428), Expect = 8.0e-158
Identity = 296/497 (59.56%), Postives = 355/497 (71.43%), Query Frame = 0


Query: 60  DESDYDSSCSSITVPDSSR--SWMSNLSFGSRRRSSSVSIYS--GADGASPHKPHKANQA 119
           +ESDY+SS      PDSSR  SW+S+ SF +    SS+S  S  G +  +  KPHKANQ 
Sbjct: 11  NESDYESSTVG---PDSSRRTSWLSS-SFTASPSCSSISHLSNHGLNSYNQSKPHKANQV 70

Query: 120 AWEAMRWLRGVKGR-VGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQ-CFYAMKVVDR 179
           AWEAM  LR   GR VGLEHFRLL+RLGSGDIG+VYLCQIR    G P+  FYAMKVVD+
Sbjct: 71  AWEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIR----GSPETAFYAMKVVDK 130

Query: 180 EALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQP 239
           EA+A++ KL RAEMEK+IL MLDHPF PTLYA F+ASHYS LVME+CPGGDLYA R RQP
Sbjct: 131 EAVAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCPGGDLYAVRLRQP 190

Query: 240 GKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVV 299
            KRF+ISS +FYAAE ++ALEYLHMMGIVYRDLKPENVL+REDGH+MLSDFDL  KCDVV
Sbjct: 191 SKRFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVMLSDFDLSFKCDVV 250

Query: 300 PKLIRSRT-----TSDAIERQVKSYTSVTSCAAAPIQPVLSCF---SAARRKRATTVTTI 359
           P+ +           D  +  ++   S  SC   P+ PV+SCF   S+ RRK+    TTI
Sbjct: 251 PQFLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSSRRRKKNVVTTTI 310

Query: 360 TEHVDGGKEEEFDPE--------------------------PELVAEPINARSKSFVGTH 419
            E+  G  +     +                          P + AEPINARSKSFVGTH
Sbjct: 311 HENAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGGCPSIFAEPINARSKSFVGTH 370

Query: 420 EYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGF 479
           EYLAPEVISGQGHGSAVDWWT GIFLYEM++G+TPFKG+NNEKTL++ILK PL FP++  
Sbjct: 371 EYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNILKAPLTFPKVIV 430

Query: 480 SSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 517
           +S +EYE+MV  +DLI KLLVKNPKKR+GSLKGS+EIKRHEFF+GVNWALIRS++PP +P
Sbjct: 431 NSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNWALIRSIKPPWVP 490

[Close]

BLAST of Sgr030527 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 431.4 bits (1108), Expect = 1.0e-120
Identity = 218/409 (53.30%), Postives = 281/409 (68.70%), Query Frame = 0


Query: 93  SSVSIYSGADGASPHKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLC 152
           S+ S +SG      +KPHKAN   WEA++ +R   G +GL HFRLL+RLG GDIG V+L 
Sbjct: 86  STCSSFSG-----NNKPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLA 145

Query: 153 QIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHY 212
           ++        +CF+AMKV+D+ ALA R KL RA+ E+EIL  LDHPFLPTLY+ F+   +
Sbjct: 146 ELHG-----TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKF 205

Query: 213 SCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVL 272
           SCLVMEFCPGGDL+  RQRQPGKRFS  +AKFY AE++LA+EYLHM+GI+YRDLKPENVL
Sbjct: 206 SCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVL 265

Query: 273 VREDGHIMLSDFDLCLKCDVVPKLIRSRTTSDAIERQVKSYTSVTSCAAAP--IQPVLSC 332
           VR+DGH+MLSDFDL L+C V P ++RS   +   ++    Y +  +C   P  I    +C
Sbjct: 266 VRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKN-SGYCAQPACIQQPSCISAPTTC 325

Query: 333 FSAARRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQ 392
           FS    +  ++ +   + +      +  P PELVAEP +ARS SFVGTHEYLAPE+I G+
Sbjct: 326 FSP---RYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGE 385

Query: 393 GHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVK 452
           GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL +++ QPL+FP     S         
Sbjct: 386 GHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FA 445

Query: 453 VEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 500
             DLI  LLVK P+ R+   +G+ E+K+H FF+GVNWAL+R   PP+IP
Sbjct: 446 ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP 473

[Close]

BLAST of Sgr030527 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 427.2 bits (1097), Expect = 1.9e-119
Identity = 217/405 (53.58%), Postives = 274/405 (67.65%), Query Frame = 0


Query: 104 ASPHKPHKANQAAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCQIRNPMVGLPQ 163
           +S +KPHKAN   WEA++ +R   G +GL HFRLL+RLG GDIG V+L ++        +
Sbjct: 78  SSINKPHKANDVRWEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAELNG-----TR 137

Query: 164 CFYAMKVVDREALAIRNKLRRAEMEKEILTMLDHPFLPTLYAEFDASHYSCLVMEFCPGG 223
           C++AMKV+D+ ALA R KL RA+ E+EIL  LDHPFLPTLY+ F+   +SCLVMEFCPGG
Sbjct: 138 CYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGG 197

Query: 224 DLYAARQRQPGKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSD 283
           DL+  RQRQPGKRF+  +AKFY AE++LA+EYLHM+GI+YRDLKPENVLVR+DGH+MLSD
Sbjct: 198 DLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSD 257

Query: 284 FDLCLKCDVVPKLIRSRTT-SDAIERQVKSYTSVTSCAAAP---IQPVLSC-----FSAA 343
           FDL L+C V   ++RS    S+ + +   S +   +C   P        SC     FS+ 
Sbjct: 258 FDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCFGPRFFSSK 317

Query: 344 RRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGS 403
            +K     T    H       +  P PELVAEP  ARS SFVGTHEYLAPE+I G+GHGS
Sbjct: 318 SKKDKKPKTENGNH-------QVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHGS 377

Query: 404 AVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQPLKFPRIGFSSSREYEEMVKVEDL 463
           AVDWWT GIFLYE+L+GKTPFKG  N  TL +++ QPL+FP     S           DL
Sbjct: 378 AVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAARDL 437

Query: 464 ISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQIP 500
           I  LLVK P+ R+   +G+ EIK+H FF+GVNWAL+R   PP+IP
Sbjct: 438 IRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIP 463

[Close]

BLAST of Sgr030527 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 422.5 bits (1085), Expect = 4.7e-118
Identity = 222/429 (51.75%), Postives = 282/429 (65.73%), Query Frame = 0


Query: 75  DSSRSWMSNLSFGSRRRSSSVSIYSGADGASPHKPHKANQAAWEAMRWLRGVKGRVGLEH 134
           D   S  S++  GS     S      +  ++ +KPHKAN   WEA++ +R   G +GL H
Sbjct: 131 DFMESGKSSICRGSTSSDVSDESSCSSFSSTVNKPHKANDLRWEAIQAVRVRDGLLGLSH 190

Query: 135 FRLLRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTM 194
           FRLL+RLG GDIG+VYL ++        +C++AMKV+D+ +LA R KL RA+ E+EIL  
Sbjct: 191 FRLLKRLGCGDIGSVYLSELSG-----TKCYFAMKVMDKTSLASRKKLLRAQTEREILQS 250

Query: 195 LDHPFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALE 254
           LDHPFLPTLY  F+   +SCLVMEFCPGGDL+  RQRQPGK FS  + KFY AE +LALE
Sbjct: 251 LDHPFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALE 310

Query: 255 YLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKLIRSRT-TSDAIERQVKSY 314
           YLHM+GIVYRDLKPENVLVREDGHIMLSDFDL L+C V P L++S    SD + + V   
Sbjct: 311 YLHMLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSAAIESDPLRKNVYCV 370

Query: 315 TSV---TSCAAAPIQPVLSCFSAARRKRATTVTTITEHVDGGKEEEFDPEPELVAEPINA 374
                  SC         +CFS    +  ++ +            +  P PELVAEP +A
Sbjct: 371 QPACIEPSCIQPSCTVPTTCFSP---RLFSSKSKKDRKPKNDTANQVRPLPELVAEPTDA 430

Query: 375 RSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISILKQ 434
           RS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+G+TPFKG  N +TL +++ Q
Sbjct: 431 RSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNVVGQ 490

Query: 435 PLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALI 494
           PL+FP     S           DLI  LL+K P++R+G  +G+ E+K+H FF+GVNWALI
Sbjct: 491 PLRFPETPVVS-------FAARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVNWALI 544

Query: 495 RSVRPPQIP 500
           R   PP+IP
Sbjct: 551 RCATPPEIP 544

[Close]

BLAST of Sgr030527 vs. TAIR 10
Match: AT3G52890.1 (KCBP-interacting protein kinase )

HSP 1 Score: 411.8 bits (1057), Expect = 8.4e-115
Identity = 220/433 (50.81%), Postives = 289/433 (66.74%), Query Frame = 0


Query: 79  SWMSNLSFGSRRRSSSVSIYSGADGAS-PHKPHKANQAAWEAMRWLRGVKGRVGLEHFRL 138
           S  S  S G   RS+S+S  S     S  +KPH +    WEA++ ++   G +GL HF L
Sbjct: 481 SLSSKDSLGDYSRSTSISEESNLSRFSCGNKPHMSMDVRWEAIKHIKVQYGSLGLRHFNL 540

Query: 139 LRRLGSGDIGNVYLCQIRNPMVGLPQCFYAMKVVDREALAIRNKLRRAEMEKEILTMLDH 198
           L++LG GDIG VYL +    ++G   C +A+KV+D E LA R K  RA+ E+EIL MLDH
Sbjct: 541 LKKLGCGDIGTVYLAE----LIG-TNCLFAIKVMDNEFLARRKKSPRAQAEREILKMLDH 600

Query: 199 PFLPTLYAEFDASHYSCLVMEFCPGGDLYAARQRQPGKRFSISSAKFYAAEIILALEYLH 258
           PFLPTLYA+F + + SCLVME+CPGGDL+  RQ+Q G+ F   +A+FY AEI+LALEYLH
Sbjct: 601 PFLPTLYAQFTSDNLSCLVMEYCPGGDLHVLRQKQLGRCFPEPAARFYVAEILLALEYLH 660

Query: 259 MMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKLIRSRTTSDAIERQVKSYTSVT 318
           M+GI+YRDLKPEN+LVREDGHIML+DFDL L+C V P L+RS +       ++    + +
Sbjct: 661 MLGIIYRDLKPENILVREDGHIMLTDFDLSLRCAVNPTLVRSNSPPGKDPARISGPYNTS 720

Query: 319 SCAAAPIQPV--------LSCFS--AARRKRATTVTTITEHVDGGKEEEFDPEPELVAEP 378
           +C    IQP         +SCFS   +  ++        +H+   ++      P+LVAEP
Sbjct: 721 NC----IQPFCITEPSCQVSCFSPRLSSNQQQGRKPKRGDHLSKTQQHLSRSLPQLVAEP 780

Query: 379 INARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLISI 438
             ARS SFVGTHEYLAPE+I G+GHG+AVDWWT G+ LYE+LYGKTPFKG NN++TL ++
Sbjct: 781 TEARSNSFVGTHEYLAPEIIKGEGHGAAVDWWTFGVLLYELLYGKTPFKGYNNDETLANV 840

Query: 439 LKQPLKFPRIGFSSSREYEEMVKVEDLISKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNW 498
           + Q LKFP     S        + +DLI  LLVK P+ R+GS KGSVEIKRH FF+G+NW
Sbjct: 841 VLQNLKFPDSPLVS-------FQAKDLIRGLLVKEPENRLGSEKGSVEIKRHPFFEGLNW 897

Query: 499 ALIRSVRPPQIPN 501
           ALIR   PP++P+
Sbjct: 901 ALIRCAIPPELPD 897

The following BLAST results are available for this feature:

BLAST of Sgr030527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946203.1	1.3e-215	82.56	protein kinase PINOID 2-like [Cucurbita moschata]	[more]
XP_022999369.1	1.8e-215	83.30	protein kinase PINOID 2-like [Cucurbita maxima]	[more]
XP_022965690.1	3.9e-215	81.37	protein kinase PINOID 2-like [Cucurbita maxima]	[more]
KAG7030399.1	8.7e-215	83.08	Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6599411.1	8.7e-215	83.08	Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Sgr030527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94E49	6.0e-158	63.09	Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV...	[more]
Q64FQ2	1.1e-156	59.56	Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1	[more]
Q9SUA3	1.4e-119	53.30	Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1...	[more]
Q9FG74	2.7e-118	53.58	Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=...	[more]
Q39183	6.7e-117	51.75	Serine/threonine-protein kinase D6PKL2 OS=Arabidopsis thaliana OX=3702 GN=D6PKL2...	[more]

BLAST of Sgr030527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G352	6.5e-216	82.56	protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450358 PE=4 ...	[more]
A0A6J1KGU7	8.5e-216	83.30	protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV...	[more]
A0A6J1HPF1	1.9e-215	81.37	protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV...	[more]
A0A6J1FGS3	6.3e-211	80.09	protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 ...	[more]
A0A5D3BGM8	4.5e-209	78.40	Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Sgr030527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26700.1	8.0e-158	59.56	AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein	[more]
AT4G26610.1	1.0e-120	53.30	D6 protein kinase like 1	[more]
AT5G55910.1	1.9e-119	53.58	D6 protein kinase	[more]
AT5G47750.1	4.7e-118	51.75	D6 protein kinase like 2	[more]
AT3G52890.1	8.4e-115	50.81	KCBP-interacting protein kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 168..188
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 370..500 e-value: 4.6E-38 score: 132.8
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 220..289 e-value: 5.0E-47 score: 162.4
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 133..219 e-value: 5.0E-47 score: 162.4
None	No IPR available	PIRSR	PIRSR037921-2	PIRSR037921-2	coord: 377..476 e-value: 39.0 score: 0.1
None	No IPR available	PIRSR	PIRSR000556-2	PIRSR000556-2	coord: 128..275 e-value: 6.7E-9 score: 31.7
None	No IPR available	PANTHER	PTHR45637:SF12	PROTEIN KINASE PINOID 2	coord: 54..506
None	No IPR available	PANTHER	PTHR45637	FLIPPASE KINASE 1-RELATED	coord: 54..506
None	No IPR available	CDD	cd05574	STKc_phototropin_like	coord: 134..499 e-value: 8.23849E-169 score: 477.885
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 135..482 e-value: 1.4E-79 score: 280.4
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 136..290 e-value: 3.2E-31 score: 108.7 coord: 372..482 e-value: 3.1E-22 score: 79.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 135..482 score: 43.424606
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 261..273
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 117..503

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr030527.1	Sgr030527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity