Sgr030305 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030305
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionbifunctional epoxide hydrolase 2-like
Locationtig00153640: 2071099 .. 2073378 (+)
RNA-Seq ExpressionSgr030305
SyntenySgr030305
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCAGATTGAGCATATCACCATCAACACCAATGGCATCAAAATGCACATTGCCTCTGTAGGGACGGGCCCAGTAGTTCTTCTTCTCCATGGCTTCCCGGAGCTCTGGTATTCATGGCGCCACCAGCTTCTGTATCTTTCTTCCGTAGGATATCGAGCTATTGCGCCGGACCTCCGCGGCTATGGCGACACGGACTCGCCGGCGTCTCCTACCTCCTACACCGCTCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGAGCTTGGGATCGAGAAGGTGTTTCTGGTCGGACATGACTGGGGGGCGATCATCGCCTGGTACTTTTGCTTGTTCAGGCCCGATAGAATCAAGGCGCTGGTGAATCTGAGCGTCCAGTTCATACCCAGAAACCCAGCGATTCCTTTCATTGAGGGTTTCAGAACTGCGTTCGGTGATGACTTCTATATGTGCAGGTTTCAGGTGAATCATAAACTTTGCTTAGGGATTAGGAATTTACTGCTATTCAATTCTGGTCCTAATTTCAATCATTTTCTGTGTTCATATTAATATCTTGTAGTTGGATGAACGGTATAATCGAATTGCTAAGCTCTGTGGTATTGTGGTGTAGATTGGCTGTGGGGACTCACATACACAATCAAAGTACAAATTGTATGCAATCACAACACTGTTAGTAAATTCAAAACTCAAAGAGATTGGTTTCTTAAACTGTTCATCACAAAGTTCTTTAGAGCTTTTTGACTGTTTCATCTTTGGTCAAATCAAATTCAGATCTATAAGCTGGTAAATCTTAAGTTAAATTATAAATTTGGTCCGACATAGTTTCAATCTAGTCCCTATAGTTTGAGCTTAATTTATAGAGACTAAATTAAAATTTTTAAAATTACATGGACTAAATTGAAATTAAGCCCAAAGCATAGAGACTAAATTGAAATTAAGCCCAAAGCATAGAGACTAAATTGAAACTATGCCCAAATCGTGGGGATCAAATTTATAATTTAACCTAAATCTTACTATTTTTAGCAAAATTTCTTGAGTCCCTTAAAAAGAGTCGTTCATCAACTTCCTGAATAAAGAATAATGGAGCCCTTTTGACCTTCTTGCAATCCTCGCTTAATTCATTAAGTTGTGTTGCTTAGAGAGTCCCATTAGATGGATTCTCTGTTTTATTATATTTTCTTTCAACAATTTCCCTCAAATTTTGAGAGCCGTTTAGAACTTTTATTTGAACACATTCTTTCCATTGAACTAGAAAGTTGGGGTTGTAGCATCTTAATGTTTTGGACCATTTTTTAACTAATAAAGCAAACTCTGATACCATTTTTGTTGGAAACATAGGGGAAACTCATATAAACAGCAAAAACACAAATGAATTGATTGTGGTTCATGTACTCATGGAATCTAAAACAACTAAGTGTAGATTAATTTCTAACAGGTTCCAGGAGAGGCAGAAGAAGATTTTGCCTCCATCGACACAGCTCAGCTGTTCAAGACATCATTATGTAATAGAAGTTCTGCACCTCCATGCTTGCCTAAAGAAATTGGATTTCGTGCGATCCCACCTCCAGAGAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTTAAGCAGACAGGCTTCACCGGAGCGTTGAACTACTATCGAGCTTTTGACCTGTAAGCAATATTTATTTATTTTCAACCTTACTTGAACAGGATCTATACTATAGGTTGTACGACTGTGACCTTGAGTTCTAAAGAGTTAGCCATTCGTATTACTCTGTTGGAACACTCTTTCTGAGTTTTAAGTTTAATAATGGTTCCATGTTTCCAAACTGAATACATGTTTTCATAAATCGGGTGAAACATCGTTTAATGTTCTAAATCTAACACATTTTCATGAGTAAAGAACTGCAGATTTGTCCCTGTTTTCTCACATGGAAATGCATGTTGTGAGACATATCTTGTAGAAGGCAGCAGATAGCATAGATGGTTATTTGGATGTATGAAGTTGAATTGATGTTTGTTTGCAGAACTTGGGAGCTCACGGCGCCATGGACAGGAGCACAGATTCAGGTACCGGTGAAGTTCATCGTCGGGGATTCGGATCTAACTTACCATTTTCCGGGAGCCAAGGAATATATCCATAATGGCGGATTCAAAAAGGACGTGCCGTTGCTGGAGGAAGTAGTTGTAGTAAAAGATGCTTGTCACTTCATCAACCAAGAAAGGCCACAAGAAATCAATGCTCACATCCATGACTTCATCAATAAATTCTGA

mRNA sequence

ATGGACCAGATTGAGCATATCACCATCAACACCAATGGCATCAAAATGCACATTGCCTCTGTAGGGACGGGCCCAGTAGTTCTTCTTCTCCATGGCTTCCCGGAGCTCTGGTATTCATGGCGCCACCAGCTTCTGTATCTTTCTTCCGTAGGATATCGAGCTATTGCGCCGGACCTCCGCGGCTATGGCGACACGGACTCGCCGGCGTCTCCTACCTCCTACACCGCTCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGAGCTTGGGATCGAGAAGGTGTTTCTGGTCGGACATGACTGGGGGGCGATCATCGCCTGGTACTTTTGCTTGTTCAGGCCCGATAGAATCAAGGCGCTGGTGAATCTGAGCGTCCAGTTCATACCCAGAAACCCAGCGATTCCTTTCATTGAGGGTTTCAGAACTGCGTTCGGTGATGACTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGAAGATTTTGCCTCCATCGACACAGCTCAGCTGTTCAAGACATCATTATGTAATAGAAGTTCTGCACCTCCATGCTTGCCTAAAGAAATTGGATTTCGTGCGATCCCACCTCCAGAGAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTTAAGCAGACAGGCTTCACCGGAGCGTTGAACTACTATCGAGCTTTTGACCTAACTTGGGAGCTCACGGCGCCATGGACAGGAGCACAGATTCAGGTACCGGTGAAGTTCATCGTCGGGGATTCGGATCTAACTTACCATTTTCCGGGAGCCAAGGAATATATCCATAATGGCGGATTCAAAAAGGACGTGCCGTTGCTGGAGGAAGTAGTTGTAGTAAAAGATGCTTGTCACTTCATCAACCAAGAAAGGCCACAAGAAATCAATGCTCACATCCATGACTTCATCAATAAATTCTGA

Coding sequence (CDS)

ATGGACCAGATTGAGCATATCACCATCAACACCAATGGCATCAAAATGCACATTGCCTCTGTAGGGACGGGCCCAGTAGTTCTTCTTCTCCATGGCTTCCCGGAGCTCTGGTATTCATGGCGCCACCAGCTTCTGTATCTTTCTTCCGTAGGATATCGAGCTATTGCGCCGGACCTCCGCGGCTATGGCGACACGGACTCGCCGGCGTCTCCTACCTCCTACACCGCTCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGAGCTTGGGATCGAGAAGGTGTTTCTGGTCGGACATGACTGGGGGGCGATCATCGCCTGGTACTTTTGCTTGTTCAGGCCCGATAGAATCAAGGCGCTGGTGAATCTGAGCGTCCAGTTCATACCCAGAAACCCAGCGATTCCTTTCATTGAGGGTTTCAGAACTGCGTTCGGTGATGACTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGAAGATTTTGCCTCCATCGACACAGCTCAGCTGTTCAAGACATCATTATGTAATAGAAGTTCTGCACCTCCATGCTTGCCTAAAGAAATTGGATTTCGTGCGATCCCACCTCCAGAGAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTTAAGCAGACAGGCTTCACCGGAGCGTTGAACTACTATCGAGCTTTTGACCTAACTTGGGAGCTCACGGCGCCATGGACAGGAGCACAGATTCAGGTACCGGTGAAGTTCATCGTCGGGGATTCGGATCTAACTTACCATTTTCCGGGAGCCAAGGAATATATCCATAATGGCGGATTCAAAAAGGACGTGCCGTTGCTGGAGGAAGTAGTTGTAGTAAAAGATGCTTGTCACTTCATCAACCAAGAAAGGCCACAAGAAATCAATGCTCACATCCATGACTTCATCAATAAATTCTGA

Protein sequence

MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNRSSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELTAPWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQERPQEINAHIHDFINKF
Homology
BLAST of Sgr030305 vs. NCBI nr
Match: XP_022984062.1 (uncharacterized protein LOC111482485 [Cucurbita maxima])

HSP 1 Score: 599.0 bits (1543), Expect = 2.4e-167
Identity = 277/316 (87.66%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH T+NTNGIKMHIAS+GTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNP I FIE FR  +GDDFYMCRFQVPGEAE DF  IDTAQLFK++L NR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDIN+YA+KFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
            PWTGAQIQVPVKFIVGD D+TYHF GAKEYIHNGGFK+DVPLLEEVV+V+DA HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           RP EIN HIHDFINKF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of Sgr030305 vs. NCBI nr
Match: XP_008454322.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29517.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 597.8 bits (1540), Expect = 5.4e-167
Identity = 278/316 (87.97%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD I+H TI TNGIKMHIASVG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNPAI FI+ FR A+GDDFYMCRFQVPGEAE DFA IDTAQLFKT+L NR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDINYYAAKFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QIQVPVKFIVGDSDLTYHF GAKEYIH GGFK+DVPLLEEVV+V++A HF+++E
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P EIN HIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Sgr030305 vs. NCBI nr
Match: XP_004152242.1 (uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical protein Csa_014782 [Cucumis sativus])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 275/316 (87.03%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD I+H TINTNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDL+GALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNPAI FI+ FR A+GDDFYMCRFQVPGEAE DFA IDTAQLFKT+L  R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDINYYAAKFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QIQVPVKFIVGD D+TYHF GAKEYIH+GGFK+DVPLLEEVV+VK+A HF+++E
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P EIN HIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Sgr030305 vs. NCBI nr
Match: XP_022934346.1 (uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothetical protein SDJN03_21281, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595.5 bits (1534), Expect = 2.7e-166
Identity = 276/316 (87.34%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH TINTNGIKMHIAS+GTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNP I FIE FR  +GDDFYMCRFQVPGEAE DF  IDTAQLFK +L NR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPE+LPSWLTEEDIN+YA+KFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
            PWTGAQIQVPVKFIVGD D+TYHF GAKEYIHNGGFK+DVPLLEEVV+V+DA HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           RP EIN HIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Sgr030305 vs. NCBI nr
Match: XP_023528764.1 (uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.1 bits (1533), Expect = 3.5e-166
Identity = 276/316 (87.34%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH TINTNGIKMHIAS+GTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNP I FIE FR  +GDDFYMCRFQVPGEAE DF  IDTAQLFK +L NR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDIN+YA+KFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
            PWTGAQIQVPVKFIVGD D+TYHF GAKEYIH GGFK+DVPLLEEVV+V+DA HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHKGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           RP EIN HIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Sgr030305 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.6e-58
Identity = 121/318 (38.05%), Postives = 184/318 (57.86%), Query Frame = 0

Query: 9   INTNGIKMHIASVGT--GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTD 68
           ++TNG+++ +   G    PVV+L HGFPEL YSWRHQ+  L+  GY  +APD RGYG + 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  SPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSV 128
            P +  +Y    +  DLVG LD++G E+   VGHDWGA++ W   L   DR+ A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 QFIPRNPAIPFIEGFRTAFGDD-FYMCRFQVPGEAEEDFASIDTAQLFKTSL------CN 188
             +PR   +P  + FR+ FG++ FY+  FQ PG A+ +    D A+  +  +       +
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNG-DPARTMRRMIGGLRPPGD 188

Query: 189 RSSAPPCL-PKEIGF-RAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTW 248
           +S+A   L P   GF   +P P  LP+W+++E++++Y  +F +TGFTG LN+YR FD  W
Sbjct: 189 QSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNW 248

Query: 249 ELTAPWTGAQIQVPVKFIVGDSD--LTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACH 308
           E TA   G  I VP  FI G +D  LT+        + +G +++        V++  A H
Sbjct: 249 ETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE--------VLIDGAGH 308

Query: 309 FINQERPQEINAHIHDFI 314
           ++ QERP E+ A + +F+
Sbjct: 309 WLQQERPGEVTAALLEFL 316

BLAST of Sgr030305 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-47
Identity = 96/298 (32.21%), Postives = 164/298 (55.03%), Query Frame = 0

Query: 13  GIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPASPT 72
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++GYG++ +P    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFIPRN 132
            Y+   +  D+V  L++LG+ +   +GHDWG ++ W   LF P+R++A+ +L+  F+P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKT--SLCNRSSAPPCLPKE 192
           P +  +E  +     D Y   FQ PG AE +    +  + FK      + +       +E
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAELEQ-NLDRTFKNFFRAHDETFLTTNRVRE 426

Query: 193 IG--FRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELTAPWTGAQI 252
           +G  F   P   +L   +TEEDI +Y  +FK++GF G LN+YR  +  W+     +G +I
Sbjct: 427 LGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKI 486

Query: 253 QVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQERPQEIN 307
            +P   +  ++DL  H P   +++ N      +P L+    +KD  H+   ++P E+N
Sbjct: 487 LIPALMVTAENDLVLH-PKMSKHMENW-----IPHLKR-GHIKDCGHWTQIDKPAELN 535

BLAST of Sgr030305 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 9.9e-47
Identity = 95/297 (31.99%), Postives = 161/297 (54.21%), Query Frame = 0

Query: 14  IKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPASPTS 73
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++GYG++ +P     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFIPRNP 133
           Y    +  ++V  LD+LG+ +   +GHDWG ++ WY  LF P+R++A+ +L+  FIP NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 AIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNRSSAPPCLPK--EI 193
            +  +E  +     D Y   FQ PG AE +    + ++ FK+       +   + K  E 
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFRAIPPPE--NLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELTAPWTGAQIQ 253
           G   +  PE  +L   +TEE+I +Y  +FK++GF G LN+YR  +  W+      G +I 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQERPQEIN 307
           +P   +  + D     P   +++ +      +P L+    ++D  H+   ++P E+N
Sbjct: 488 IPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKR-GHIEDCGHWTQMDKPTEVN 535

BLAST of Sgr030305 vs. ExPASy Swiss-Prot
Match: P95276 (Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1988 PE=1 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 1.9e-45
Identity = 114/359 (31.75%), Postives = 167/359 (46.52%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVG----TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIA 60
           M Q+ H  +N  G ++H  +       GP+V+LLHGFPE WYSWRHQ+  L+  GYR +A
Sbjct: 1   MSQV-HRILNCRGTRIHAVADSPPDQQGPLVVLLHGFPESWYSWRHQIPALAGAGYRVVA 60

Query: 61  PDLRGYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPD 120
            D RGYG +       +Y    +VGD+VG LD  G E+ F+VGHDWGA +AW F    PD
Sbjct: 61  IDQRGYGRSSKYRVQKAYRIKELVGDVVGVLDSYGAEQAFVVGHDWGAPVAWTFAWLHPD 120

Query: 121 RIKALVNLSVQFIPRN----PAIPF-----------IEGFRTAFGDDFYMCRFQVPGEAE 180
           R   +V +SV F  R     P  PF           + G    +  D++  +  +  E E
Sbjct: 121 RCAGVVGISVPFAGRGVIGLPGSPFGERRPSDYHLELAGPGRVWYQDYFAVQDGIITEIE 180

Query: 181 EDF-------------------------ASIDTAQLFKTSLCNRSSAPPCLPKEIGFR-A 240
           ED                          A +D   +    +    + P C+ +    + A
Sbjct: 181 EDLRGWLLGLTYTVSGEGMMAATKAAVDAGVDLESMDPIDVIR--AGPLCMAEGARLKDA 240

Query: 241 IPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELTAPWTGAQIQVPVKFI 300
              PE +P+W TE D+++Y  +F+++GF G L++Y   D  W   A   G  +  P  FI
Sbjct: 241 FVYPETMPAWFTEADLDFYTGEFERSGFGGPLSFYHNIDNDWHDLADQQGKPLTPPALFI 300

Query: 301 VGDSDL-TYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQERPQEINAHIHDFI 314
            G  D+ T     A E  H     + +P      ++ D  H+I QE P+E N  + DF+
Sbjct: 301 GGQYDVGTIWGAQAIERAH-----EVMPNYRGTHMIADVGHWIQQEAPEETNRLLLDFL 351

BLAST of Sgr030305 vs. ExPASy Swiss-Prot
Match: I6YC03 (Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephB PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.9e-45
Identity = 114/359 (31.75%), Postives = 167/359 (46.52%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVG----TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIA 60
           M Q+ H  +N  G ++H  +       GP+V+LLHGFPE WYSWRHQ+  L+  GYR +A
Sbjct: 1   MSQV-HRILNCRGTRIHAVADSPPDQQGPLVVLLHGFPESWYSWRHQIPALAGAGYRVVA 60

Query: 61  PDLRGYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPD 120
            D RGYG +       +Y    +VGD+VG LD  G E+ F+VGHDWGA +AW F    PD
Sbjct: 61  IDQRGYGRSSKYRVQKAYRIKELVGDVVGVLDSYGAEQAFVVGHDWGAPVAWTFAWLHPD 120

Query: 121 RIKALVNLSVQFIPRN----PAIPF-----------IEGFRTAFGDDFYMCRFQVPGEAE 180
           R   +V +SV F  R     P  PF           + G    +  D++  +  +  E E
Sbjct: 121 RCAGVVGISVPFAGRGVIGLPGSPFGERRPSDYHLELAGPGRVWYQDYFAVQDGIITEIE 180

Query: 181 EDF-------------------------ASIDTAQLFKTSLCNRSSAPPCLPKEIGFR-A 240
           ED                          A +D   +    +    + P C+ +    + A
Sbjct: 181 EDLRGWLLGLTYTVSGEGMMAATKAAVDAGVDLESMDPIDVIR--AGPLCMAEGARLKDA 240

Query: 241 IPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELTAPWTGAQIQVPVKFI 300
              PE +P+W TE D+++Y  +F+++GF G L++Y   D  W   A   G  +  P  FI
Sbjct: 241 FVYPETMPAWFTEADLDFYTGEFERSGFGGPLSFYHNIDNDWHDLADQQGKPLTPPALFI 300

Query: 301 VGDSDL-TYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQERPQEINAHIHDFI 314
            G  D+ T     A E  H     + +P      ++ D  H+I QE P+E N  + DF+
Sbjct: 301 GGQYDVGTIWGAQAIERAH-----EVMPNYRGTHMIADVGHWIQQEAPEETNRLLLDFL 351

BLAST of Sgr030305 vs. ExPASy TrEMBL
Match: A0A6J1J139 (uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 277/316 (87.66%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH T+NTNGIKMHIAS+GTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNP I FIE FR  +GDDFYMCRFQVPGEAE DF  IDTAQLFK++L NR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDIN+YA+KFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
            PWTGAQIQVPVKFIVGD D+TYHF GAKEYIHNGGFK+DVPLLEEVV+V+DA HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           RP EIN HIHDFINKF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of Sgr030305 vs. ExPASy TrEMBL
Match: A0A5D3E0A4 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001200 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.6e-167
Identity = 278/316 (87.97%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD I+H TI TNGIKMHIASVG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNPAI FI+ FR A+GDDFYMCRFQVPGEAE DFA IDTAQLFKT+L NR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDINYYAAKFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QIQVPVKFIVGDSDLTYHF GAKEYIH GGFK+DVPLLEEVV+V++A HF+++E
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P EIN HIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Sgr030305 vs. ExPASy TrEMBL
Match: A0A1S3BZJ8 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.6e-167
Identity = 278/316 (87.97%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD I+H TI TNGIKMHIASVG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNPAI FI+ FR A+GDDFYMCRFQVPGEAE DFA IDTAQLFKT+L NR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDINYYAAKFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QIQVPVKFIVGDSDLTYHF GAKEYIH GGFK+DVPLLEEVV+V++A HF+++E
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P EIN HIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Sgr030305 vs. ExPASy TrEMBL
Match: A0A0A0KT70 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001920 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.6e-167
Identity = 275/316 (87.03%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD I+H TINTNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDL+GALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNPAI FI+ FR A+GDDFYMCRFQVPGEAE DFA IDTAQLFKT+L  R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPENLPSWLTEEDINYYAAKFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QIQVPVKFIVGD D+TYHF GAKEYIH+GGFK+DVPLLEEVV+VK+A HF+++E
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P EIN HIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Sgr030305 vs. ExPASy TrEMBL
Match: A0A6J1F1J8 (uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC111441539 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 276/316 (87.34%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH TINTNGIKMHIAS+GTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP S TSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVNLSVQF PRNP I FIE FR  +GDDFYMCRFQVPGEAE DF  IDTAQLFK +L NR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           S+  PCLPKE GFRAIPPPE+LPSWLTEEDIN+YA+KFK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
            PWTGAQIQVPVKFIVGD D+TYHF GAKEYIHNGGFK+DVPLLEEVV+V+DA HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPQEINAHIHDFINKF 317
           RP EIN HIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Sgr030305 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 471.5 bits (1212), Expect = 5.4e-133
Identity = 201/316 (63.61%), Postives = 254/316 (80.38%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M++IEH TI+TNGI MH+AS+G+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDTDSPASPTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGD+D+P S  SYT LHIVGDLVG LD LG+++VFLVGHDWGAI+AW+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSLCNR 180
           LVN SV F PRNP++  ++ FR  FGDD+Y+CRFQ PGE EEDFA +DT +L      +R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 SSAPPCLPKEIGFRAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRAFDLTWELT 240
           +  PPC+PK +GFR +P P +LP+WLTE+D+ +Y  KF Q GFTG LNYYRA +L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 APWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKDACHFINQE 300
           APWTG QI+VPVKFIVGD D+TY+ PG KEYIH GG KK VP L+EVVV++   HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 RPQEINAHIHDFINKF 317
           +P E+  HI+ F  KF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Sgr030305 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 5.6e-114
Identity = 186/323 (57.59%), Postives = 236/323 (73.07%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIASVG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M+ I+H  ++ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDTDSPASPTSYTALHIVGDLVGALDEL--GIEKVFLVGHDWGAIIAWYFCLFRPD 120
           LRGYGD+DSP S + YT L++VGDLV  LD +    EKVFLVGHDWGAII W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKALVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTS 180
           +I   V LSV +  RNP +  ++GF+  FGDD+Y+CRFQ PG+ E + AS D     +  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LCNRSSAPPCLPKEIGFRAIPPPEN----LPSWLTEEDINYYAAKFKQTGFTGALNYYRA 240
              R+  PP LPK+  F   P P +    LP W +++D+++Y +KF++ GFTG LNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDLTWELTAPWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKD 300
            DL WELTAPWTGA+IQVPVKF+ GD D+ Y  PG KEYIH GGF  DVP L+E+VV++D
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 ACHFINQERPQEINAHIHDFINK 316
           A HF+NQE+PQE+ AHI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Sgr030305 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 8.7e-107
Identity = 176/324 (54.32%), Postives = 230/324 (70.99%), Query Frame = 0

Query: 1   MDQIEHITINTNGIKMHIA-----SVGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 60
           +D +EH T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDTDSPASPTSYTALHIVGDLVGALDEL--GIEKVFLVGHDWGAIIAWYFCLF 120
           APDLRGYGDT++P     YT L++ GD+V  +D +  G + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKALVNLSVQFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLF 180
           RP+++KALVN+SV F PRNP    +   R  FGDD+Y+CRFQ  GE E +F  + T  + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KTSLCNRSSAPPCLPKEIGF-RAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRA 240
           K  L  ++  P  LPK+  F R+      LP WLT+ED++YY  K++  GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDLTWELTAPWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKD 300
            D  WELTAPWTGA+I+VPVKFI+GD DLTY+FPGAKEYI+ GGFK+DVPLL+E VV+K 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 ACHFINQERPQEINAHIHDFINKF 317
             HF+++E P  IN HIH+F +KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of Sgr030305 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 371.3 bits (952), Expect = 7.6e-103
Identity = 177/325 (54.46%), Postives = 230/325 (70.77%), Query Frame = 0

Query: 4   IEHITINTNGIKMHIASVG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  +  NGI +H+A  G   GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDTDSPASPTSYTALHIVGDLVGALDELGI---EKVFLVGHDWGAIIAWYFCLFRPDRI 123
           YGD+D+PA  +SYT  +IVGDL+  +  L     EKVF+VGHDWGA+IAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSV--QFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTS 183
           KALVNLSV   F P +P++  ++  R  +GDD+Y+CRFQ  G+ E + A + T ++ K  
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LCNRSSAPPCLPKEIGF-----RAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYR 243
           L  R+  P  +PK+  F       IP    LPSWLTEED+ Y+ +KF++ GF+G +NYYR
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFEEKGFSGPVNYYR 240

Query: 244 AFDLTWELTAPWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVK 303
            F+   EL  PW G++IQVP KF++G+ DL Y+ PG KEYIH   FK+DVPLLEE VV++
Sbjct: 241 NFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVME 300

Query: 304 DACHFINQERPQEINAHIHDFINKF 317
              HFINQE+PQEI   I DFI+KF
Sbjct: 301 GVAHFINQEKPQEILQIILDFISKF 321

BLAST of Sgr030305 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 7.9e-100
Identity = 172/324 (53.09%), Postives = 227/324 (70.06%), Query Frame = 0

Query: 4   IEHITINTNGIKMHIASVG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  +  NGI +H+A  G   G +VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDTDSPASPTSYTALHIVGDLVGALDELGIE--KVFLVGHDWGAIIAWYFCLFRPDRIK 123
           YGD+D+PA  +S+T  +IVGDLV  +  L  E  KVF+VGHDWGA+IAWY CLFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--QFIPRNPAIPFIEGFRTAFGDDFYMCRFQVPGEAEEDFASIDTAQLFKTSL 183
           ALVNLSV   F P +P++  ++  R  +G+D+Y+CRFQ  G+ E + A + T ++ K  L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 CNRSSAPPCLPKEIGF-----RAIPPPENLPSWLTEEDINYYAAKFKQTGFTGALNYYRA 243
             R+  P  +PK+  F       IP    LPSWLTEED+ Y+ +KFK+ GF G +NYYR 
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFKEKGFCGPVNYYRN 240

Query: 244 FDLTWELTAPWTGAQIQVPVKFIVGDSDLTYHFPGAKEYIHNGGFKKDVPLLEEVVVVKD 303
           F+   EL  PW G++IQVP KF++G+ DL Y+ PG KEYIH   FK+DVPL+EE VV++ 
Sbjct: 241 FNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEG 300

Query: 304 ACHFINQERPQEINAHIHDFINKF 317
             HF+NQE+PQEI   I DFI+ F
Sbjct: 301 VAHFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022984062.12.4e-16787.66uncharacterized protein LOC111482485 [Cucurbita maxima][more]
XP_008454322.15.4e-16787.97PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bi... [more]
XP_004152242.11.6e-16687.03uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical ... [more]
XP_022934346.12.7e-16687.34uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothet... [more]
XP_023528764.13.5e-16687.34uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
I6YGS05.6e-5838.05Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C23.4e-4732.21Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349139.9e-4731.99Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P952761.9e-4531.75Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX... [more]
I6YC031.9e-4531.75Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Match NameE-valueIdentityDescription
A0A6J1J1391.2e-16787.66uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485... [more]
A0A5D3E0A42.6e-16787.97Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZJ82.6e-16787.97bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE... [more]
A0A0A0KT707.6e-16787.03AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A6J1F1J81.3e-16687.34uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
Match NameE-valueIdentityDescription
AT4G02340.15.4e-13363.61alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.15.6e-11457.59alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.18.7e-10754.32alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.17.6e-10354.46soluble epoxide hydrolase [more]
AT2G26750.17.9e-10053.09alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 111..124
score: 26.85
coord: 97..110
score: 33.0
coord: 51..66
score: 44.61
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..181
e-value: 7.1E-23
score: 81.6
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 51..66
score: 60.8
coord: 97..110
score: 52.92
coord: 290..312
score: 36.96
coord: 31..49
score: 55.26
coord: 111..124
score: 49.35
coord: 250..266
score: 38.24
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..316
e-value: 1.5E-106
score: 358.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..315
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR43329:SF94HYDROLASE, PUTATIVE-RELATEDcoord: 1..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030305.1Sgr030305.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity