Sgr030271 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030271
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionMethenyltetrahydrofolate cyclohydrolase
Locationtig00153640: 1753704 .. 1758001 (+)
RNA-Seq ExpressionSgr030271
SyntenySgr030271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGCTCAACATGTTTGTGGTGATGAAAGTAGTGAGTCATGTAAAACCTTTGGGAAGAAACTTGCTCAAGCTTACCAGAATGTGAAGGTTGCCATGGCGCAAAACCGAGGCAGTGGAGTTGGAACGAGACGAAACCAAAGAGAAGATCCCATTAGAACCATAATGTTCTTGGGATCATGGAGTCATACATAAGTTAATTAAGGCTTGTCGGGTAAATTGGTTTTCAAAACTGTATTTTAAGAACTGTGATTAAATGATTTGTAAATGTTAGTACTATTTTGTATGTTGAAGGTATCAATAACAAACCCGTAATTTTAAAAGGAAGAATAACTTCATTATTTTCAAAATCAGATCTTTCATGAAAACACTGCTAAAAGATAAAACAGTTATCCAAATATGTTTTTACTTAATTTTTGTTGTAAAAAAAGTGTTATGAAAGGAGTTTTCAAATAAGCCCTTAATATCTTTTTTCTATCGTGCAAGTAGTTGAGAAATACAATATCATAATTTGTATTTTAATCCAACTTATATGTCATCTCCCTACATCAAAATCAATGTAAATAAATTATCCGCTAAGTGTTGTATTATCTCCCTCTATCCCACACCCTCTCCCTCGCACCGTTCTTACTATTTTTTTCATGCCAATCTAAGCAGAGCTCAACTGATTAAAACATTAGTAACATCTCCTAAATGTTAGAGATTCAAATTCCCACCTCACCCTTTGTTTAAATTTTAAAAAAATGATAAAAATAAAAATTTGCCTTTATAACTTTTTCGAAAAATTGTGTTTTCTTACTTTTATAATAACATAAAAGTATAAATCAATGTTTCAATATGATTATAAAAGTAATGAAATAAATTAGTTAGTTTATCAATCACTCACTTTCAATGCACACCTTTTTTAATTTAATATTTTATTATATTTTTAGTTATTTCTTTTCAAATGTATATTAAAAAACACTGGATCGACAAACTTAGCTATCAATATTTACTTTGTTAATAATGCTAAATAGCAATTTTCTCAATTTATCATATTCTAGTGATTGGACATCTTTGATTTATAAACCATTAAAAATACAATATGGTCTTTAGTTCTTGGAATATTTTTGCAACAAGATTCTCTTCAATTTTCCAATCCATCTATTTTTTTCTCTTTGGCCCCAACTCTGGAGTAAACCCTAACTTTAAATTGTTTACAAAATAATTTGTCCACATCATCTCCACGTAGATATTCGTTGCTTGTACTAAGACAAGTAGTGCCCAAATTAAACACTACAAGTTAATTCAACATTAAATTTATCAATAGTTCTAACAACGTGGACTATTTGTACATTTATTTAAAAGTTGAGGTACTAAAATAAAATTTAAAAAAAAATTAGGGACAAAAATAAAAAATAGAAGCCTGGCAAAAGCCCAGTAGATTCCTGGCAAAAGCCCATTTTTCACGAGCCACAGTCCATCACCCAAATCGAAGCCTGGCAAAAGCCCAGCAGATTCCTCAAGAGCGATTCCATGGTTCCAACTCAGTGAGTCTACAATCTATCCTTCTCAATCTCAACTCAACTCAACTCAACTCATCCACACTGTTCTGCTTCTGCCATTGTCACTGGCAGAAATCTTGAAGGGATAAGCATTTCTCCTTGTCAGCTGCAAAAATGGCGTCCGATTCCGAGCACAAAGCTACGATAATTGACGGCAAAGAGATCGCCCAGACCGTCAGATCTGAAATAGCTGAGGAAGTAAACAAACTCTCTGAGAAATACGGAAAGGTTTTTTTCTGCTTTCGAAATTTCCTGGGCTCGAGATATTCTTACGATTTCAATCTACTATTGTGAAAAATTGTGTGTGTATCTGTCTAATTTTAGCATTTTCTTGTTCCTTATGGTGTGGGATTTGCGAAGGTTCCGGGACTGGCGGTGGTGATTGTGGGGAGTAGAAAGGATTCACTGAGCTATGTGAAGATGAAGAGAAAGGCTTGCGCGGAAGTCGGAATCAAGTCCTTCGATTTTGATCTACCTGAACAAGTGTCTGAGGCCGAATTGATCAGCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTTAATCTCTTGTGTACTTTTTATGCTTAGGATTTCTTGGATTTTTAGTGCCGCAGTACAAATTTGATAGAGTCTCCACTTACTTCGAAATAGTATAATGAAGATGCCTTTCTGCTAGATAAATGTTACTTAGATCCATTTTTCCAATGTGTGTAATAAATGTAATTGGAGCTTTTTAGCATGTGAACTTTTTAGCTTTCTTAAAGCTTCTAGTTTTAATATTTAGAGGGAGTTTGAAATAATTTTTAAGAGTAAAATACTTTTCTTATAAGCATTTTTGGAAGAAGCACTGGCGTTTCCATCAGAAGCACTTTTAAAGTGCTTTTTAGTATCTTTAGAATTTTTAAAATCACTTTTTACTATGTTAACAAACACCCTATTATTCACTGTTCCAAAAGTCATACAAAGTTACCCTGAACATGTGATATTGGAATTTTTTTGCATTTAAATTTTAAAGATCTGATTTTTCTTTTTTAATTCTTTGAAATTTGAAAACTTTCGTTCAAATTTTTTCTCCAACAAATTGATTTGTTTGTTGCTTTGACAGGTATATTGGTTCAGCTTCCTTTGCCAAAACACATAAATGAAGAGAGAGTTTTAGCTGAAATCAGCATCGAGAAGGATGTAGATGGCTTTCATCCTCTGAATATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGCAATAGCAAATGTTTTATAAGCCTTAGAAAATCATTCAACTCATTCTTAATAAATCTCTGTCAACTGGAAGAGCAATAGCTCCTTGTTTATCAATGTTCAAGATCTGGATTTACGTAGATCTTAGCTCAAAAAATGGCAATTCATATCATAAATTCGCTTCATAGTCTGAGATAATGTTTTATGCGCAGGGGTGTCTCGAGCTGCTGTCACGAAGTGGAATAAGTATTAGGGGGAAGAAGGCAGTTGTTATGGGGCGAAGCAACATCGTTGGATTACCAGTTTCATTGCTGCTTCTTAAAGCAGATGCAACTGTGACAATTGTCCATTCGCGCTCTGTTGATCCAGAAAGCGTTATCCGCGAAGCAGACATTGTAATTGCGGCAGCAGGACAGCCACAAATGGTATGCTTGGTTTGACAATGGCATTGTTTCCCCTTCCCCAGTTCTTATCTTTGAACATCTGTTTGGCTTCACTGAGAATTTCTGACTTTGGTTCCCAAGTGTTCTCGGGAGCAACACTTTTAGGAAATATGATGAGGCGCTACAATAGCACTAGAACAAAATTCAGAGACCTTAAAACACTGTCATATGTAGGATGGAAACATAAGTGAAAGAGCATTAGGAAAAAGGCATGGTTACTGTAGCCAGTGATCTATGCAATATTCTTCTTGGCATGTTAGCAATGAGGAAAATATTTTGTCATGTTTCTTAGTATTTGATTTCATGTTAAATCACCACTAAACCTAAAAGCTTAAGTTGATGGTTTAAACCATAGTTATACATATGCTTAACACTCTTCTTCACTTGTGGGTTTAAAAACGAGTATGAGACCCAATAAGTGATTAAATTATCAATGTCAATTGAGGAGAACATGACATGGTAGCTAATACTATATTGAATCACCACTAAACCCAAAAATTTATGTTGATAGGTTAATCTTTTATACGCATTCTTAATAGATTAAATTATAAGTTTGGATTCAAAACTTTCAAAATTGTGTTTAATAGATCTCTGGACTTTAAAAAGTGTCTAACATGTCTTTGAATTTTTTATTTTGTGTCTAATAAATCTTCGACTTATGAGACTTTTTAAAGTTTCGAAACTTATTGGACACAAAATTGAAAGTTTAGAGACTTAATTAAACACTCTTTAAAGCATAATTCTGAAAGTTTAGAAACCAAACTTAATAGTTTAATCCTATCAATATTTTGTTTGCTTGTCGAAGCAATTGAGTGAGAGACTTACTGGTGAATATGGTTGTGGTTGATGTTAGATCAAGGGCAGTTGGATCAAACCAGGTGCTGCAGTCATAGATGTTGGCACAAATGCAGTTGATGACCCAAGCAAAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAGGCTTGTAAGGTGGCTGGATGGATAACTCCTGTTCCCGGTGGTGTGGGCCCAATGACTGTTGCCATGCTGCTTCGGAATACTTTGGATGGTGCTAAGCGGGTGATCCAGCAGTGA

mRNA sequence

ATGAAGGCTCAACATGTTTGTGGTGATGAAAGTAGTGAGTCATGTAAAACCTTTGGGAAGAAACTTGCTCAAGCTTACCAGAATGTGAAGGTTGCCATGGCGCAAAACCGAGGCAGTGGAGTTGGAACGAGACGAAACCAAAGAGAAGATCCCATTAGAACCATAATGTTCTTGGGATCATGGACTGCAAAAATGGCGTCCGATTCCGAGCACAAAGCTACGATAATTGACGGCAAAGAGATCGCCCAGACCGTCAGATCTGAAATAGCTGAGGAAGTAAACAAACTCTCTGAGAAATACGGAAAGGTTCCGGGACTGGCGGTGGTGATTGTGGGGAGTAGAAAGGATTCACTGAGCTATGTGAAGATGAAGAGAAAGGCTTGCGCGGAAGTCGGAATCAAGTCCTTCGATTTTGATCTACCTGAACAAGTGTCTGAGGCCGAATTGATCAGCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTATATTGGTTCAGCTTCCTTTGCCAAAACACATAAATGAAGAGAGAGTTTTAGCTGAAATCAGCATCGAGAAGGATGTAGATGGCTTTCATCCTCTGAATATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGGGTGTCTCGAGCTGCTGTCACGAAGTGGAATAAGTATTAGGGGGAAGAAGGCAGTTGTTATGGGGCGAAGCAACATCGTTGGATTACCAGTTTCATTGCTGCTTCTTAAAGCAGATGCAACTGTGACAATTGTCCATTCGCGCTCTGTTGATCCAGAAAGCGTTATCCGCGAAGCAGACATTGTAATTGCGGCAGCAGGACAGCCACAAATGATCAAGGGCAGTTGGATCAAACCAGGTGCTGCAGTCATAGATGTTGGCACAAATGCAGTTGATGACCCAAGCAAAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAGGCTTGTAAGGTGGCTGGATGGATAACTCCTGTTCCCGGTGGTGTGGGCCCAATGACTGTTGCCATGCTGCTTCGGAATACTTTGGATGGTGCTAAGCGGGTGATCCAGCAGTGA

Coding sequence (CDS)

ATGAAGGCTCAACATGTTTGTGGTGATGAAAGTAGTGAGTCATGTAAAACCTTTGGGAAGAAACTTGCTCAAGCTTACCAGAATGTGAAGGTTGCCATGGCGCAAAACCGAGGCAGTGGAGTTGGAACGAGACGAAACCAAAGAGAAGATCCCATTAGAACCATAATGTTCTTGGGATCATGGACTGCAAAAATGGCGTCCGATTCCGAGCACAAAGCTACGATAATTGACGGCAAAGAGATCGCCCAGACCGTCAGATCTGAAATAGCTGAGGAAGTAAACAAACTCTCTGAGAAATACGGAAAGGTTCCGGGACTGGCGGTGGTGATTGTGGGGAGTAGAAAGGATTCACTGAGCTATGTGAAGATGAAGAGAAAGGCTTGCGCGGAAGTCGGAATCAAGTCCTTCGATTTTGATCTACCTGAACAAGTGTCTGAGGCCGAATTGATCAGCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTATATTGGTTCAGCTTCCTTTGCCAAAACACATAAATGAAGAGAGAGTTTTAGCTGAAATCAGCATCGAGAAGGATGTAGATGGCTTTCATCCTCTGAATATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGGGTGTCTCGAGCTGCTGTCACGAAGTGGAATAAGTATTAGGGGGAAGAAGGCAGTTGTTATGGGGCGAAGCAACATCGTTGGATTACCAGTTTCATTGCTGCTTCTTAAAGCAGATGCAACTGTGACAATTGTCCATTCGCGCTCTGTTGATCCAGAAAGCGTTATCCGCGAAGCAGACATTGTAATTGCGGCAGCAGGACAGCCACAAATGATCAAGGGCAGTTGGATCAAACCAGGTGCTGCAGTCATAGATGTTGGCACAAATGCAGTTGATGACCCAAGCAAAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAGGCTTGTAAGGTGGCTGGATGGATAACTCCTGTTCCCGGTGGTGTGGGCCCAATGACTGTTGCCATGCTGCTTCGGAATACTTTGGATGGTGCTAAGCGGGTGATCCAGCAGTGA

Protein sequence

MKAQHVCGDESSESCKTFGKKLAQAYQNVKVAMAQNRGSGVGTRRNQREDPIRTIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ
Homology
BLAST of Sgr030271 vs. NCBI nr
Match: XP_022143417.1 (bifunctional protein FolD 2-like [Momordica charantia])

HSP 1 Score: 564.3 bits (1453), Expect = 7.6e-157
Identity = 286/299 (95.65%), Postives = 295/299 (98.66%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MASDS HKAT+IDGKEIAQ VRSEIAEEVNKLSEK+GKVPGLAVVIVGSRKDS SYVKMK
Sbjct: 1   MASDSGHKATLIDGKEIAQAVRSEIAEEVNKLSEKHGKVPGLAVVIVGSRKDSQSYVKMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFDFDLPEQVSEAEL++KIHELNANPEVHGILVQLPLPKHINEE+VLAEI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELVAKIHELNANPEVHGILVQLPLPKHINEEKVLAEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSVDPESV+READIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVDPESVVREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. NCBI nr
Match: XP_023524684.1 (bifunctional protein FolD 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.8 bits (1444), Expect = 8.4e-156
Identity = 284/299 (94.98%), Postives = 295/299 (98.66%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+SEHKATIIDGK+IAQTVRSEIAEEV KLSEKYGKVPGLAVVIVGSRKDS SYV MK
Sbjct: 1   MASESEHKATIIDGKQIAQTVRSEIAEEVKKLSEKYGKVPGLAVVIVGSRKDSQSYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFD+DLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEE+VL+EI
Sbjct: 61  RKACAEVGIKSFDYDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           +IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 NIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSV+PESVIREADIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVNPESVIREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEACKVAGW+TPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. NCBI nr
Match: XP_004152261.2 (bifunctional protein FolD 2 [Cucumis sativus])

HSP 1 Score: 559.3 bits (1440), Expect = 2.4e-155
Identity = 283/310 (91.29%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 54  TIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGS 113
           TI F     AKMAS+S+HKATIIDGK+IAQTVRSEI EEVNKLS+KYGK+PGLAVVIVG+
Sbjct: 66  TIFFFS--PAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGN 125

Query: 114 RKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPK 173
           RKDSL+YV MKRKAC EVGIKSF+ DLPEQVSEAELISK+HELNANPEVHGILVQLPLP 
Sbjct: 126 RKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPN 185

Query: 174 HINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKK 233
           HINEE+VL+EISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGC+ELLSRSGISIRGKK
Sbjct: 186 HINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKK 245

Query: 234 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWI 293
           AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADI+IAAAGQ QMIKGSWI
Sbjct: 246 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWI 305

Query: 294 KPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 353
           KPGAAVIDVGTNAVDDP+KKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT
Sbjct: 306 KPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 365

Query: 354 LDGAKRVIQQ 364
           LDGAKRVI+Q
Sbjct: 366 LDGAKRVIEQ 373

BLAST of Sgr030271 vs. NCBI nr
Match: XP_022997744.1 (bifunctional protein FolD 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 558.5 bits (1438), Expect = 4.2e-155
Identity = 285/299 (95.32%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+SEHKATIIDGK+IAQTVRSEIAEEV KLSEKYGKVPGLAVVIVGSRKDS SYV MK
Sbjct: 1   MASESEHKATIIDGKQIAQTVRSEIAEEVKKLSEKYGKVPGLAVVIVGSRKDSQSYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEE+VL+EI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           +IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 NIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSV+PESVIREADIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVNPESVIREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEA KVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEASKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. NCBI nr
Match: XP_022948445.1 (bifunctional protein FolD 2 [Cucurbita moschata] >KAG6606974.1 Bifunctional protein FolD 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.4 bits (1435), Expect = 9.3e-155
Identity = 284/299 (94.98%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+SEHKATIIDGK+IAQTVRSEIAEEV KLSEKYGKVPGLAVVIVGSRKDS SYV MK
Sbjct: 1   MASESEHKATIIDGKQIAQTVRSEIAEEVKKLSEKYGKVPGLAVVIVGSRKDSQSYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFD+DLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEE+VL+EI
Sbjct: 61  RKACAEVGIKSFDYDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           +IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 NIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSV+PESVIREADIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVNPESVIREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEA KVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEASKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. ExPASy Swiss-Prot
Match: Q9LHH7 (Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 2.8e-138
Identity = 247/296 (83.45%), Postives = 271/296 (91.55%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS S+H A IIDGK IA T+RSEIAEEV  LSEK+GKVPGLAVVIVGSRKDS +YV  K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFD  LPE+VSEA+LISK+HELN+NP+VHGILVQLPLPKHINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           SI+KDVDGFHPLNIGKLAMKGR+PLFLPCTPKGCLELL+RSG+ I+G++AVV+GRSNIVG
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVT VHS + DPE++IREADIVIAA GQ  MIKG+WIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 361
           NAV DPSKKSGYRLVGDVDF EA KVAG+ITPVPGGVGPMTVAMLLRNT+DGAKRV
Sbjct: 241 NAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

BLAST of Sgr030271 vs. ExPASy Swiss-Prot
Match: O65271 (Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD4 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 8.3e-106
Identity = 190/326 (58.28%), Postives = 242/326 (74.23%), Query Frame = 0

Query: 35  QNRGSGVGTRRNQREDPIRTIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVN 94
           Q   SG G        PI +I           + SE  A +IDGK +A+ +R EI  EV+
Sbjct: 39  QTTASGRGCCIRSSSSPISSI--------SADTKSEGGAIVIDGKAVAKKIRDEITIEVS 98

Query: 95  KLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIH 154
           ++ E  G +PGLAV++VG RKDS +YV+ K+KAC  VGIKSF+  L E  SE E++  + 
Sbjct: 99  RMKESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVS 158

Query: 155 ELNANPEVHGILVQLPLPKHINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCT 214
             N +P VHGILVQLPLP H++E+ +L  +SIEKDVDGFHPLNIG+LAM+GR+PLF+PCT
Sbjct: 159 GFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCT 218

Query: 215 PKGCLELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIRE 274
           PKGC+ELL R  I I+GK+AVV+GRSNIVG+P +LLL + DATV+I+HSR+ +PE + RE
Sbjct: 219 PKGCIELLHRYNIEIKGKRAVVIGRSNIVGMPAALLLQREDATVSIIHSRTKNPEEITRE 278

Query: 275 ADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWI 334
           ADI+I+A GQP M++GSWIKPGA +IDVG N V+DPS   GYRLVGD+ ++EA KVA  I
Sbjct: 279 ADIIISAVGQPNMVRGSWIKPGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAI 338

Query: 335 TPVPGGVGPMTVAMLLRNTLDGAKRV 361
           TPVPGGVGPMT+AMLL NTL  AKR+
Sbjct: 339 TPVPGGVGPMTIAMLLSNTLTSAKRI 356

BLAST of Sgr030271 vs. ExPASy Swiss-Prot
Match: A2RVV7 (Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FOLD1 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 8.8e-100
Identity = 176/293 (60.07%), Postives = 230/293 (78.50%), Query Frame = 0

Query: 68  DSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKA 127
           ++E K  +IDG  IA+ +R++I  EV K+ +  GKVPGLAVV+VG ++DS +YV+ K KA
Sbjct: 58  ETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKA 117

Query: 128 CAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIE 187
           C E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP+H+NE ++L  + +E
Sbjct: 118 CEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLE 177

Query: 188 KDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPV 247
           KDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSNIVGLP+
Sbjct: 178 KDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPM 237

Query: 248 SLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAV 307
           SLLL + DATV+ VH+ + DPE + R+ADIVIAAAG P +++GSW+KPGA VIDVGT  V
Sbjct: 238 SLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTTPV 297

Query: 308 DDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 361
           +D S + GYRLVGDV ++EA  VA  ITPVPGGVGPMT+ MLL NTL+ AKR+
Sbjct: 298 EDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKRI 350

BLAST of Sgr030271 vs. ExPASy Swiss-Prot
Match: O65269 (Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD3 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 3.7e-82
Identity = 159/325 (48.92%), Postives = 209/325 (64.31%), Query Frame = 0

Query: 36  NRGSGVGTRRNQREDPIRTIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVNK 95
           NR      R +  +  +RT       T  + S S   A +IDGK  A+ +R +I  EV++
Sbjct: 18  NRNGVFLPRPSVSQFSLRTTASTWRCTLSIRSSSSPSAIVIDGKAEAKKIRDDIKIEVSR 77

Query: 96  LSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIHE 155
           + E  G VP                                     E  SE E++  +  
Sbjct: 78  MKESIGVVPA------------------------------------EDSSEEEVLKYVSG 137

Query: 156 LNANPEVHGILVQLPLPKHINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTP 215
            N +P VHG+LVQLPLP H++E+ +L  +SIEKDVDGFHPLNIG+LAM+GR+PLF+PCTP
Sbjct: 138 FNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTP 197

Query: 216 KGCLELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREA 275
           KGC+ELL R  I  +GK+AVV+GRSNIVG+P +LLL K DATV+I+HSR+++PE + R+A
Sbjct: 198 KGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKEDATVSIIHSRTMNPEELTRQA 257

Query: 276 DIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWIT 335
           DI+I+A G+P M++GSWIKPGA +IDVG   V+DPS   G RLVGD+ + EA K+A  IT
Sbjct: 258 DILISAVGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAGGERLVGDICYVEASKIASAIT 306

Query: 336 PVPGGVGPMTVAMLLRNTLDGAKRV 361
           PVPG VGPMT+AMLL NTL  AKR+
Sbjct: 318 PVPGDVGPMTIAMLLSNTLTSAKRI 306

BLAST of Sgr030271 vs. ExPASy Swiss-Prot
Match: A5GAM5 (Bifunctional protein FolD OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=folD PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.4e-81
Identity = 160/287 (55.75%), Postives = 204/287 (71.08%), Query Frame = 0

Query: 73  ATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKACAEVG 132
           A IIDGK IA  +R EI  EV KL+ K G  PGLAVV+VG    S  YV MK KAC +VG
Sbjct: 2   AKIIDGKAIAAKIRGEITAEVAKLASK-GVTPGLAVVLVGEDPASKVYVSMKEKACKDVG 61

Query: 133 IKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIEKDVDG 192
           I S ++ LP   SEA+L+  IH+LN++P++HGIL+QLPLPK I+ E+VL  IS EKD DG
Sbjct: 62  IFSDEYKLPVDTSEADLLLLIHKLNSDPKIHGILIQLPLPKQIDTEKVLEAISPEKDADG 121

Query: 193 FHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLL 252
           FHP N+G+L +    PLF PCTP G + +L  +G+ + GK+ VV+GRSNIVG PV+ + L
Sbjct: 122 FHPYNVGRLVI--GKPLFQPCTPYGVMVMLKEAGVELAGKEVVVVGRSNIVGKPVAFMCL 181

Query: 253 KADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAVDDPSK 312
           + +ATVT+ HS++ D  + +  AD+VIAA GQP+MIKG+WIK GA VIDVG N V +   
Sbjct: 182 QQNATVTLCHSKTRDLAAKVGMADVVIAAVGQPEMIKGAWIKEGAVVIDVGVNRVGEK-- 241

Query: 313 KSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKR 360
               +LVGDV+F  A + A  ITPVPGGVGPMT+ MLL NTL+ AKR
Sbjct: 242 ----KLVGDVEFDAAAERASAITPVPGGVGPMTITMLLYNTLEAAKR 279

BLAST of Sgr030271 vs. ExPASy TrEMBL
Match: A0A6J1CQR5 (Methenyltetrahydrofolate cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111013297 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.7e-157
Identity = 286/299 (95.65%), Postives = 295/299 (98.66%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MASDS HKAT+IDGKEIAQ VRSEIAEEVNKLSEK+GKVPGLAVVIVGSRKDS SYVKMK
Sbjct: 1   MASDSGHKATLIDGKEIAQAVRSEIAEEVNKLSEKHGKVPGLAVVIVGSRKDSQSYVKMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFDFDLPEQVSEAEL++KIHELNANPEVHGILVQLPLPKHINEE+VLAEI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELVAKIHELNANPEVHGILVQLPLPKHINEEKVLAEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSVDPESV+READIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVDPESVVREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. ExPASy TrEMBL
Match: A0A0A0KWH1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G001540 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 283/310 (91.29%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 54  TIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGS 113
           TI F     AKMAS+S+HKATIIDGK+IAQTVRSEI EEVNKLS+KYGK+PGLAVVIVG+
Sbjct: 66  TIFFFS--PAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGN 125

Query: 114 RKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPK 173
           RKDSL+YV MKRKAC EVGIKSF+ DLPEQVSEAELISK+HELNANPEVHGILVQLPLP 
Sbjct: 126 RKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPN 185

Query: 174 HINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKK 233
           HINEE+VL+EISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGC+ELLSRSGISIRGKK
Sbjct: 186 HINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKK 245

Query: 234 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWI 293
           AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADI+IAAAGQ QMIKGSWI
Sbjct: 246 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWI 305

Query: 294 KPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 353
           KPGAAVIDVGTNAVDDP+KKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT
Sbjct: 306 KPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 365

Query: 354 LDGAKRVIQQ 364
           LDGAKRVI+Q
Sbjct: 366 LDGAKRVIEQ 373

BLAST of Sgr030271 vs. ExPASy TrEMBL
Match: A0A6J1KCF4 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492609 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.0e-155
Identity = 285/299 (95.32%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+SEHKATIIDGK+IAQTVRSEIAEEV KLSEKYGKVPGLAVVIVGSRKDS SYV MK
Sbjct: 1   MASESEHKATIIDGKQIAQTVRSEIAEEVKKLSEKYGKVPGLAVVIVGSRKDSQSYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEE+VL+EI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           +IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 NIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSV+PESVIREADIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVNPESVIREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEA KVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEASKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. ExPASy TrEMBL
Match: A0A6J1G9V8 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452127 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.5e-155
Identity = 284/299 (94.98%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+SEHKATIIDGK+IAQTVRSEIAEEV KLSEKYGKVPGLAVVIVGSRKDS SYV MK
Sbjct: 1   MASESEHKATIIDGKQIAQTVRSEIAEEVKKLSEKYGKVPGLAVVIVGSRKDSQSYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFD+DLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEE+VL+EI
Sbjct: 61  RKACAEVGIKSFDYDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           +IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 NIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSV+PESVIREADIVIAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVNPESVIREADIVIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP++KSGYRLVGDVDFQEA KVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI+Q
Sbjct: 241 NAVDDPTRKSGYRLVGDVDFQEASKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

BLAST of Sgr030271 vs. ExPASy TrEMBL
Match: A0A5D3E076 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00860 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.9e-155
Identity = 280/299 (93.65%), Postives = 293/299 (97.99%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS+S+HKATIIDGK+IAQTVRSE+AEEVNKLSEKYGKVPGLAVVIVG+RKDSL+YV MK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKAC EVGIKSF+ DLPEQVSEAELISK+HELNANPEVHGILVQLPLP HINEE+VL+EI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVTIVHSRSVDPESVIREADI+IAAAGQ QMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIQQ 364
           NAVDDP+KKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKR I+Q
Sbjct: 241 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRAIEQ 299

BLAST of Sgr030271 vs. TAIR 10
Match: AT3G12290.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.0e-139
Identity = 247/296 (83.45%), Postives = 271/296 (91.55%), Query Frame = 0

Query: 65  MASDSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMK 124
           MAS S+H A IIDGK IA T+RSEIAEEV  LSEK+GKVPGLAVVIVGSRKDS +YV  K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 125 RKACAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEI 184
           RKACAEVGIKSFD  LPE+VSEA+LISK+HELN+NP+VHGILVQLPLPKHINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 185 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 244
           SI+KDVDGFHPLNIGKLAMKGR+PLFLPCTPKGCLELL+RSG+ I+G++AVV+GRSNIVG
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVG 180

Query: 245 LPVSLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGT 304
           LPVSLLLLKADATVT VHS + DPE++IREADIVIAA GQ  MIKG+WIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGT 240

Query: 305 NAVDDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 361
           NAV DPSKKSGYRLVGDVDF EA KVAG+ITPVPGGVGPMTVAMLLRNT+DGAKRV
Sbjct: 241 NAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

BLAST of Sgr030271 vs. TAIR 10
Match: AT4G00620.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 385.2 bits (988), Expect = 5.9e-107
Identity = 190/326 (58.28%), Postives = 242/326 (74.23%), Query Frame = 0

Query: 35  QNRGSGVGTRRNQREDPIRTIMFLGSWTAKMASDSEHKATIIDGKEIAQTVRSEIAEEVN 94
           Q   SG G        PI +I           + SE  A +IDGK +A+ +R EI  EV+
Sbjct: 39  QTTASGRGCCIRSSSSPISSI--------SADTKSEGGAIVIDGKAVAKKIRDEITIEVS 98

Query: 95  KLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKACAEVGIKSFDFDLPEQVSEAELISKIH 154
           ++ E  G +PGLAV++VG RKDS +YV+ K+KAC  VGIKSF+  L E  SE E++  + 
Sbjct: 99  RMKESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVS 158

Query: 155 ELNANPEVHGILVQLPLPKHINEERVLAEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCT 214
             N +P VHGILVQLPLP H++E+ +L  +SIEKDVDGFHPLNIG+LAM+GR+PLF+PCT
Sbjct: 159 GFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCT 218

Query: 215 PKGCLELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIRE 274
           PKGC+ELL R  I I+GK+AVV+GRSNIVG+P +LLL + DATV+I+HSR+ +PE + RE
Sbjct: 219 PKGCIELLHRYNIEIKGKRAVVIGRSNIVGMPAALLLQREDATVSIIHSRTKNPEEITRE 278

Query: 275 ADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAVDDPSKKSGYRLVGDVDFQEACKVAGWI 334
           ADI+I+A GQP M++GSWIKPGA +IDVG N V+DPS   GYRLVGD+ ++EA KVA  I
Sbjct: 279 ADIIISAVGQPNMVRGSWIKPGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAI 338

Query: 335 TPVPGGVGPMTVAMLLRNTLDGAKRV 361
           TPVPGGVGPMT+AMLL NTL  AKR+
Sbjct: 339 TPVPGGVGPMTIAMLLSNTLTSAKRI 356

BLAST of Sgr030271 vs. TAIR 10
Match: AT2G38660.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 365.2 bits (936), Expect = 6.3e-101
Identity = 176/293 (60.07%), Postives = 230/293 (78.50%), Query Frame = 0

Query: 68  DSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKA 127
           ++E K  +IDG  IA+ +R++I  EV K+ +  GKVPGLAVV+VG ++DS +YV+ K KA
Sbjct: 58  ETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKA 117

Query: 128 CAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIE 187
           C E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP+H+NE ++L  + +E
Sbjct: 118 CEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLE 177

Query: 188 KDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPV 247
           KDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSNIVGLP+
Sbjct: 178 KDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPM 237

Query: 248 SLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAV 307
           SLLL + DATV+ VH+ + DPE + R+ADIVIAAAG P +++GSW+KPGA VIDVGT  V
Sbjct: 238 SLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTTPV 297

Query: 308 DDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 361
           +D S + GYRLVGDV ++EA  VA  ITPVPGGVGPMT+ MLL NTL+ AKR+
Sbjct: 298 EDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKRI 350

BLAST of Sgr030271 vs. TAIR 10
Match: AT2G38660.3 (Amino acid dehydrogenase family protein )

HSP 1 Score: 365.2 bits (936), Expect = 6.3e-101
Identity = 176/293 (60.07%), Postives = 230/293 (78.50%), Query Frame = 0

Query: 68  DSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKA 127
           ++E K  +IDG  IA+ +R++I  EV K+ +  GKVPGLAVV+VG ++DS +YV+ K KA
Sbjct: 58  ETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKA 117

Query: 128 CAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIE 187
           C E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP+H+NE ++L  + +E
Sbjct: 118 CEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLE 177

Query: 188 KDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPV 247
           KDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSNIVGLP+
Sbjct: 178 KDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPM 237

Query: 248 SLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAV 307
           SLLL + DATV+ VH+ + DPE + R+ADIVIAAAG P +++GSW+KPGA VIDVGT  V
Sbjct: 238 SLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTTPV 297

Query: 308 DDPSKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 361
           +D S + GYRLVGDV ++EA  VA  ITPVPGGVGPMT+ MLL NTL+ AKR+
Sbjct: 298 EDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKRI 350

BLAST of Sgr030271 vs. TAIR 10
Match: AT2G38660.4 (Amino acid dehydrogenase family protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 153/260 (58.85%), Postives = 204/260 (78.46%), Query Frame = 0

Query: 68  DSEHKATIIDGKEIAQTVRSEIAEEVNKLSEKYGKVPGLAVVIVGSRKDSLSYVKMKRKA 127
           ++E K  +IDG  IA+ +R++I  EV K+ +  GKVPGLAVV+VG ++DS +YV+ K KA
Sbjct: 58  ETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKA 117

Query: 128 CAEVGIKSFDFDLPEQVSEAELISKIHELNANPEVHGILVQLPLPKHINEERVLAEISIE 187
           C E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP+H+NE ++L  + +E
Sbjct: 118 CEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLE 177

Query: 188 KDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVGLPV 247
           KDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSNIVGLP+
Sbjct: 178 KDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPM 237

Query: 248 SLLLLKADATVTIVHSRSVDPESVIREADIVIAAAGQPQMIKGSWIKPGAAVIDVGTNAV 307
           SLLL + DATV+ VH+ + DPE + R+ADIVIAAAG P +++GSW+KPGA VIDVGT  V
Sbjct: 238 SLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTTPV 297

Query: 308 DDPSKKSGYRLVGDVDFQEA 328
           +D S + GYRLVGDV ++EA
Sbjct: 298 EDSSCEFGYRLVGDVCYEEA 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143417.17.6e-15795.65bifunctional protein FolD 2-like [Momordica charantia][more]
XP_023524684.18.4e-15694.98bifunctional protein FolD 2 [Cucurbita pepo subsp. pepo][more]
XP_004152261.22.4e-15591.29bifunctional protein FolD 2 [Cucumis sativus][more]
XP_022997744.14.2e-15595.32bifunctional protein FolD 2 isoform X2 [Cucurbita maxima][more]
XP_022948445.19.3e-15594.98bifunctional protein FolD 2 [Cucurbita moschata] >KAG6606974.1 Bifunctional prot... [more]
Match NameE-valueIdentityDescription
Q9LHH72.8e-13883.45Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1[more]
O652718.3e-10658.28Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
A2RVV78.8e-10060.07Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
O652693.7e-8248.92Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
A5GAM55.4e-8155.75Bifunctional protein FolD OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1CQR53.7e-15795.65Methenyltetrahydrofolate cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KWH11.2e-15591.29Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G0015... [more]
A0A6J1KCF42.0e-15595.32Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492... [more]
A0A6J1G9V84.5e-15594.98Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3E0765.9e-15593.65Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT3G12290.12.0e-13983.45Amino acid dehydrogenase family protein [more]
AT4G00620.15.9e-10758.28Amino acid dehydrogenase family protein [more]
AT2G38660.16.3e-10160.07Amino acid dehydrogenase family protein [more]
AT2G38660.36.3e-10160.07Amino acid dehydrogenase family protein [more]
AT2G38660.41.4e-8758.85Amino acid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolasePRINTSPR00085THFDHDRGNASEcoord: 180..201
score: 55.42
coord: 317..333
score: 48.64
coord: 334..352
score: 85.22
coord: 227..247
score: 55.68
coord: 104..126
score: 50.67
coord: 145..172
score: 53.85
coord: 276..305
score: 55.77
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolaseHAMAPMF_01576THF_DHG_CYHcoord: 73..361
score: 37.962849
NoneNo IPR availableGENE3D3.40.50.10860Leucine Dehydrogenase, chain A, domain 1coord: 78..354
e-value: 1.5E-115
score: 386.8
NoneNo IPR availableGENE3D3.40.50.720coord: 76..337
e-value: 1.5E-115
score: 386.8
NoneNo IPR availablePANTHERPTHR48099C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATEDcoord: 54..361
NoneNo IPR availablePANTHERPTHR48099:SF10BIFUNCTIONAL PROTEIN FOLD 1, MITOCHONDRIALcoord: 54..361
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 73..192
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainPFAMPF02882THF_DHG_CYH_Ccoord: 194..359
e-value: 1.9E-66
score: 222.3
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainCDDcd01080NAD_bind_m-THF_DH_Cyclohydcoord: 186..358
e-value: 1.58803E-93
score: 274.818
IPR020630Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domainPFAMPF00763THF_DHG_CYHcoord: 75..191
e-value: 1.3E-41
score: 141.5
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00766THF_DHG_CYH_1coord: 146..171
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00767THF_DHG_CYH_2coord: 338..346
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 193..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030271.1Sgr030271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005829 cytosol
molecular_function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
molecular_function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
molecular_function GO:0003824 catalytic activity