Sgr030270 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr030270
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	tig00153640: 1746608 .. 1749275 (+)
RNA-Seq Expression	Sgr030270
Synteny	Sgr030270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGTGAATCCTCTCTCAAGCTTCCCCTCATAGACTTCTCCAACATAGAAATAGGGAGTCCCAAGTGGGAGTTGGTGAGAGCACAAGTGAAGGAAGCACTTGAGGAGTTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGTGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAGTTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGCCAATACCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATGTCTGAAAAGGTCCAAAACTTCACTAAGATCTTGTGGCCTCAGGGCAACCCAACCTTTAGGTATGTGTGTGTATATATATGTGTTCTTTCTTTTTTCTTTTGATTCTTTTCTTGAATATTTTGAAACAGGATTAGGGTTTCGAGATTTGGTTTTTAGTTTTTGAGGTTGTTTGGTAACATTCTTATTATTATTTTTCTTGTTTCTAATTTTTGAGATATATGTGGTTGGTAACTTATTTCCTTGTTTCTTATTTCATTTTTCTAATTTTTGAGAAAGTTTATGGTAACTGATTTTTGTTTCTTGTTTCCATTTTTTAGAAACGTCTCCATAATTGTATATAAATTTTGGAAATATAATTTTTTTTTTGTTTCTTGTTTCTTATTTTTATTTTTTTAGTAAAACATAAATAAAAGTATTATTTTATCATTTTTGACTTATAAAATGTATAGTAAACATAAATTAAAATTAAAATCAAACAATAATTTCGAATATTTTATTTTTTCAAGTAAAACTTCCATTTTTTTAATATAAGTTTTTATTCAACAAGAAATATATTTACCAAATAAGTTTTTATTTTTCTTTCTTAACCTTTGAAAAACAAGAAACATGAAATAAGAAAAGAAAGAAAAGAAAAGTTACCCAATGGAATTGGTGTAATTTTTTTCTTAATTATGAAGTCAAACTAATTTTTTAAAATTACTACGATTTGTCTCTTTAATCATAAGAATTATTTCCTGTATTTTCCATATATTTACACTACATATCAACTTAAAACTATACAACACAGATCAATGAAAGAAAACACACGAAAACAACAAAATACTGTTTTTTGTATTCTAAAACATCAGTTTCGCAAGATCAATCCATACTTGTTTCCAAATCTTTTTTTTTTTTTTTTGTTGGCAGCAAAGTGATAGAATCCTATTCAAAACAATTATCAGAACTAGACCAAATAGTGAGGAGGATGGTTTTGGAGAGTTTGGGTGTTGAGAAATACTTGAAGGAACATTTGGGGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGCCCTCAGAGCAATGAGACCAAGCTTGGCCTTCATCCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAATCATGTTGAAGGTTTGCAGATACAAGCCAAAGATGGCAGATGGATTAACTTCAAACCTTCCCCACACTCTTTTGTTGCCATGATTGGAGATTCCCTCCATGTGAGTAACTTTTAATTAGTGTTTACCTAACTTTTTGTTTGACAGCGATTTTGTTTTTCGTTCTATGTTTCTTACGAGATGAGTTTGTTTGCTGATGATTCATTTGATCCTTGATATTAGTCCTAGAAACGTTTGAAAATCAAATCTAGGGTTTTTTTTTTTCTTATAAAATGTAGTTTTCAAAAACTTTTGTGTGTTGTTTTTTGTTTCGAGATGAGGTTAAGATTATTTGTTTTAAATCTTTACTTTAATTCCTGAATTTTCATGCTTATTTTATTTTGGTTTCTTGAACTTTTATGTTTATTCCTATTTTGGTCTCTAAACTCTACATAAAAACCATTTTAGTCCTTATTCTCATTTTTTCAAAATTTGTTTAACAGAAACATGACGTAACATGCATATTGTTCATTTATGTGATGAGAAGATGTCTATATTGATAAATATTTAAAACTACATGGAGAGAGTCAATTGTTTGGTTTTGTAAGAGATCAAGATTCTAATAAAATTTTAAATGAAAAATTAACGACAATAACTAAAATAAGTTTTTATGCAAAGTTTAGGGACTAAAACAGAATATCCTGAAAGTCTCTAAAAGGCTTAAAATAAAGCAAGTATGAAAGTTCATAAACAAAGACATAATTCAAACTCTTATTTTCAAAAAGTAGAACATGAAACAATTTTTTTTTTACTATACTATCAAATAAGTCCTTAATTATAGAGAACCCAACCAACCATGCAAAATATGGTTGATCCACCCTAGTTGAAAGTAAAAAGCACCTTACATTTGGGCAGATTTATCGACATATTTAGAGTACTTTTATATTATTACTTAGAAGAATTTATTTAAGTGACAGTCACGTTAAGACAATCGTGTTATCGTAGGCATGGACGAATGGGAGATTGCATTCTCCGTATCATAGAGTGATGATGAGTGGAAATGAGGCAAGGTATTCAGCTGGGCTGTTCTCAATCCCAAGAGCAGGCTATACAATCAAGGCACCAGAAGAGCTTGTAGATGAAGAACACCCTTTACTCTTCAAGCCATTTGATCATGTGGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAGAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCTTAA

mRNA sequence

ATGGATGGTGAATCCTCTCTCAAGCTTCCCCTCATAGACTTCTCCAACATAGAAATAGGGAGTCCCAAGTGGGAGTTGGTGAGAGCACAAGTGAAGGAAGCACTTGAGGAGTTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGTGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAGTTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGCCAATACCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATGTCTGAAAAGGTCCAAAACTTCACTAAGATCTTGTGGCCTCAGGGCAACCCAACCTTTAGCAAAGTGATAGAATCCTATTCAAAACAATTATCAGAACTAGACCAAATAGTGAGGAGGATGGTTTTGGAGAGTTTGGGTGTTGAGAAATACTTGAAGGAACATTTGGGGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGCCCTCAGAGCAATGAGACCAAGCTTGGCCTTCATCCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAATCATGTTGAAGGTTTGCAGATACAAGCCAAAGATGGCAGATGGATTAACTTCAAACCTTCCCCACACTCTTTTGTTGCCATGATTGGAGATTCCCTCCATGCATGGACGAATGGGAGATTGCATTCTCCGTATCATAGAGTGATGATGAGTGGAAATGAGGCAAGGTATTCAGCTGGGCTGTTCTCAATCCCAAGAGCAGGCTATACAATCAAGGCACCAGAAGAGCTTGTAGATGAAGAACACCCTTTACTCTTCAAGCCATTTGATCATGTGGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAGAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCTTAA

Coding sequence (CDS)

ATGGATGGTGAATCCTCTCTCAAGCTTCCCCTCATAGACTTCTCCAACATAGAAATAGGGAGTCCCAAGTGGGAGTTGGTGAGAGCACAAGTGAAGGAAGCACTTGAGGAGTTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGTGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAGTTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGCCAATACCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATGTCTGAAAAGGTCCAAAACTTCACTAAGATCTTGTGGCCTCAGGGCAACCCAACCTTTAGCAAAGTGATAGAATCCTATTCAAAACAATTATCAGAACTAGACCAAATAGTGAGGAGGATGGTTTTGGAGAGTTTGGGTGTTGAGAAATACTTGAAGGAACATTTGGGGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGCCCTCAGAGCAATGAGACCAAGCTTGGCCTTCATCCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAATCATGTTGAAGGTTTGCAGATACAAGCCAAAGATGGCAGATGGATTAACTTCAAACCTTCCCCACACTCTTTTGTTGCCATGATTGGAGATTCCCTCCATGCATGGACGAATGGGAGATTGCATTCTCCGTATCATAGAGTGATGATGAGTGGAAATGAGGCAAGGTATTCAGCTGGGCTGTTCTCAATCCCAAGAGCAGGCTATACAATCAAGGCACCAGAAGAGCTTGTAGATGAAGAACACCCTTTACTCTTCAAGCCATTTGATCATGTGGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAGAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCTTAA

Protein sequence

MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNPTFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQRAQSALKTYCGA

Homology

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BLAST of Sgr030270 vs. NCBI nr
Match: XP_038906019.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 6.1e-163
Identity = 277/312 (88.78%), Postives = 300/312 (96.15%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD E+SLKLP IDFSN+E+GSPKWE+V+AQVKEALEEFGCFEASF+KVPLEVRK LFEAL
Sbjct: 1   MDSETSLKLPSIDFSNLELGSPKWEMVKAQVKEALEEFGCFEASFDKVPLEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPL+TKLRNVS+KPFHGYVGQYP+APLFESMGVDDST+ EKVQ+FT ILWPQGN 
Sbjct: 61  EELFNLPLQTKLRNVSKKPFHGYVGQYPLAPLFESMGVDDSTLPEKVQSFTNILWPQGNS 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFSKVIESYS+QLSELDQ+VRRMVLESLGVEKYL+EHL STNYLLRVMKYKGPQSNET+L
Sbjct: 121 TFSKVIESYSQQLSELDQMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGPQSNETQL 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WINF+PSP+SFVAMIGDSLHAWTNGRLHSP
Sbjct: 181 GLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPNSFVAMIGDSLHAWTNGRLHSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
           YHRVMMSG EARYSAGLFSIP+AGY IKAP+ELVDE+HPLLF PFDHVEFLQFYYTEAGQ
Sbjct: 241 YHRVMMSGGEARYSAGLFSIPKAGYIIKAPKELVDEQHPLLFNPFDHVEFLQFYYTEAGQ 300

Query: 301 RAQSALKTYCGA 313
           RAQSALKT+CGA
Sbjct: 301 RAQSALKTFCGA 312

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BLAST of Sgr030270 vs. NCBI nr
Match: XP_008454372.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 579.3 bits (1492), Expect = 2.0e-161
Identity = 274/313 (87.54%), Postives = 297/313 (94.89%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MDGESSLKLPLIDFSN+E G PKWEL +AQVKEALEEFGCFEASF+KVP+EVRK LFEAL
Sbjct: 1   MDGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDST+ +KVQ+FT ILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKG-PQSNETK 180
           TFSKVIE+YS+QLSELD++VRRMVLESLGVEKY++EHL STNYLLRVMKYKG  QSNETK
Sbjct: 121 TFSKVIETYSQQLSELDEMVRRMVLESLGVEKYMEEHLESTNYLLRVMKYKGTDQSNETK 180

Query: 181 LGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHS 240
           +GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WINF+PSPHSFVAMIGDS HAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPHSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAG 300
           PYHRVMMSG E RYSAGLFSIP+AGY +KAPEELVDE+HPLLF PFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QRAQSALKTYCGA 313
           Q+AQSALKT+CGA
Sbjct: 301 QKAQSALKTFCGA 313

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BLAST of Sgr030270 vs. NCBI nr
Match: XP_022143397.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Momordica charantia])

HSP 1 Score: 575.9 bits (1483), Expect = 2.2e-160
Identity = 277/312 (88.78%), Postives = 292/312 (93.59%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD ESSLKLPLIDFSN+E+GSPKWE+ +A VK+ALEEFGCFEASFEKV LE RK LFEAL
Sbjct: 1   MDCESSLKLPLIDFSNLELGSPKWEMAKALVKKALEEFGCFEASFEKVLLEARKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGV+DSTM EKVQ FT ILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVEDSTMLEKVQTFTNILWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFS+VIESYSKQLSELDQIVRRM+LESLGVEKYL+EHL STNYLLRVMKYKGPQS ETK+
Sbjct: 121 TFSQVIESYSKQLSELDQIVRRMILESLGVEKYLEEHLESTNYLLRVMKYKGPQSKETKI 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLHPHTDKN VTILYQN VEGLQIQ KDG WI+FKPSP SFVAMIGDSLHAWTNGRLHSP
Sbjct: 181 GLHPHTDKNFVTILYQNQVEGLQIQTKDGTWISFKPSPQSFVAMIGDSLHAWTNGRLHSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
            HRVMMSG EARYSAGLFS+P+AGY IKAP+ELVDEEHPLLFKPFDHVEFLQFYYT+AGQ
Sbjct: 241 RHRVMMSGKEARYSAGLFSVPKAGYIIKAPKELVDEEHPLLFKPFDHVEFLQFYYTQAGQ 300

Query: 301 RAQSALKTYCGA 313
            AQSALKTYCGA
Sbjct: 301 TAQSALKTYCGA 312

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BLAST of Sgr030270 vs. NCBI nr
Match: XP_004152262.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8649036.1 hypothetical protein Csa_014516 [Cucumis sativus])

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 269/313 (85.94%), Postives = 295/313 (94.25%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD ESSLKLPLIDFSN+E G PKWEL +AQVKEALEEFGCFEASF+KVP+EVRK LFEAL
Sbjct: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDST+ +KVQ+FT ILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSN-ETK 180
           TFSKV+E+YS+QL+ELD++VRRMVLESLGVEKYL+EHL STNYLLRVMKYKG +SN ETK
Sbjct: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180

Query: 181 LGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHS 240
           +GLH HTDKNIVTILYQNHV+GLQ++ KDG+WINF+PSP SFVAMIGDS HAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAG 300
           PYHRVMMSG E RYSAGLFSIP+AGY +KAPEELVDE+HPLLF PFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QRAQSALKTYCGA 313
           Q+AQSALKTYCGA
Sbjct: 301 QKAQSALKTYCGA 313

[Close]

BLAST of Sgr030270 vs. NCBI nr
Match: XP_023528402.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.9 bits (1439), Expect = 2.7e-155
Identity = 265/312 (84.94%), Postives = 291/312 (93.27%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD E SLKLP IDFS++E GSPKWEL +A VKEALEEFGCFEASF+KV  EVRK LFEAL
Sbjct: 1   MDSEPSLKLPHIDFSDLEAGSPKWELAKALVKEALEEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDST+ EKVQ+F  I WPQGNP
Sbjct: 61  EELFNLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFS+VIESYS+QLSELDQIVRRMV+ESLGVEKY++EHL STNYLLRVMKYKGP+SN+T+L
Sbjct: 121 TFSEVIESYSQQLSELDQIVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESNDTQL 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WI+F+PSP+SFV MIGDS HAWTNGRL+SP
Sbjct: 181 GLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
           YHRV+MSG +ARYSAGLFSIP+AGY IKAP+ELVDEEHPLLF PFDHVEFLQFYYTEAGQ
Sbjct: 241 YHRVVMSGEKARYSAGLFSIPKAGYMIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAGQ 300

Query: 301 RAQSALKTYCGA 313
           RAQSALKTYCGA
Sbjct: 301 RAQSALKTYCGA 312

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BLAST of Sgr030270 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 9.3e-98
Identity = 173/312 (55.45%), Postives = 231/312 (74.04%), Query Frame = 0


Query: 6   SLKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEEL 65
           S +LP+IDFS  N++ GS KW+ V+A V +ALE++GCFEA F+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G+DD+ + EKV +FT+ LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGP-----QSNE 185
           S+ I  +S+QL ELD +VRRM++ES G+E Y+ EHL ST YL R+MKY  P        E
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 190

Query: 186 TKLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRL 245
           TKLGL  HTDKNI+TIL+Q  V+GL+++ KD +WI  KPS  S + M+GDSL A  NGRL
Sbjct: 191 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRL 250

Query: 246 HSPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTE 305
           HSPYHRV+M+G + RYS GLFSIP+ G  I +PEELVD+EHP +FKPF++ +FL F+ TE
Sbjct: 251 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 310

Query: 306 AGQRAQSALKTY 310
           AG+ AQSAL  +
Sbjct: 311 AGRIAQSALHAF 318

[Close]

BLAST of Sgr030270 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.1e-97
Identity = 173/313 (55.27%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 6   SLKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEEL 65
           S +LP+IDFS  N++ GS KW+ V A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + EKV +FT+ LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGP------QSN 185
           S+ I  +S+QL ELD +VRRM++ES G+E Y+ EHL ST YL R+MKY  P         
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGR 245
           ETKLGL  HTDKNI+TIL+Q  V+GL+++ KD +WI  KPS  S + M+GDSL A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYT 305
           LHSPYHRV+M+G + RYS GLFSIP+ G  I +PEELVD+EHP +FKPF++ +FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQRAQSALKTY 310
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]

BLAST of Sgr030270 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 9.6e-95
Identity = 168/302 (55.63%), Postives = 225/302 (74.50%), Query Frame = 0


Query: 6   SLKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEEL 65
           S +LP+IDFS  N++ GS KW+ V A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP-QGNPTF 125
           F LP+ TK RNVS K FHGY+       L+ES+G++D+ + EKV +FT+ LWP  GN + 
Sbjct: 64  FELPIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 123

Query: 126 SKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGP----QSNET 185
           S+ I  +S+QL ELD +VRRM++ES G+EKY+ EHL ST YL R+MKY  P       ET
Sbjct: 124 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 183

Query: 186 KLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLH 245
           KLGL  HTDKNI+TIL+Q  V+GL+++ KD +WI  KPS  S + M+GDSL A  NGRLH
Sbjct: 184 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRLH 243

Query: 246 SPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEA 301
           SPYHRV+M+G + RYS GLFSIP+ G  I +PEELVD+EHP +FKPF++ +FL F+ TEA
Sbjct: 244 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 301

[Close]

BLAST of Sgr030270 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.5e-74
Identity = 148/396 (37.37%), Postives = 223/396 (56.31%), Query Frame = 0


Query: 3   GESSLKLPLIDFSN--IEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 62
           G  SL+LPLI+ ++  +E GS KW  VR+ V++ALE+FGCFEAS++KV LE+++ + + +
Sbjct: 2   GSCSLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTM 61

Query: 63  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP--QG 122
           EELF LP+ETK RNV  KP+ GY+    ++   ES+G+ ++ + E +  FT+ LWP   G
Sbjct: 62  EELFALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENINEFTQQLWPHGDG 121

Query: 123 NPTFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSN-- 182
           N   SK I+ ++++L E+D +VRRMV+ES G+EKY+ +HL ST Y    +   G + N  
Sbjct: 122 NENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTAYATDELNIVGVEPNVG 181

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 182 VKVNADISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVGVNVN 241

Query: 243 ---------------------ETKLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFK 302
                                E KLGL  HTDKN+ T+L+Q+ +EGL+++ KD +WI  K
Sbjct: 242 SNVAVNAKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVK 301

Query: 303 PSPHSFVAMIGDSLHAWTNGRLHSPYHRVMMSGNE-ARYSAGLFSIPRAGYTIKAPEELV 311
           PSP++F+ + GDSL A  NGR+ +PYHRV ++  +  RY+A +F+ P+  Y I+AP+ELV
Sbjct: 302 PSPNTFIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELV 361

[Close]

BLAST of Sgr030270 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.7e-73
Identity = 152/429 (35.43%), Postives = 227/429 (52.91%), Query Frame = 0


Query: 3   GESSLKLPLIDFSN--IEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 62
           G  SL+LPLI+ ++  +E GS KW  VR+ V++ALE+FGCFEAS++KV LE+++ + + +
Sbjct: 2   GSCSLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTM 61

Query: 63  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP--QG 122
           EELF LP+ETK RNV  KP+ GY+    ++   ES+G+ ++ + E V  FT+ LWP   G
Sbjct: 62  EELFALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENVNEFTQQLWPDGDG 121

Query: 123 NPTFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGP----- 182
           N   SK I+ ++++L E+D +VRRMV+ES G+EKY+ +HL ST Y +R+MKY  P     
Sbjct: 122 NENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDA 181

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 182 NTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLN 241

Query: 243 ---------------------------------------------------QSNETKLGL 302
                                                               + E KLGL
Sbjct: 242 VDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGL 301

Query: 303 HPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSPYH 311
             HTDKN+ T+L+Q+ +EGL+++ KD +WI  KPSP++F+ + GDSL A  NGR+ +PYH
Sbjct: 302 PCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNTFIVIAGDSLCALMNGRIRAPYH 361

[Close]

BLAST of Sgr030270 vs. ExPASy TrEMBL
Match: A0A1S3BYJ6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103494791 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 9.5e-162
Identity = 274/313 (87.54%), Postives = 297/313 (94.89%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MDGESSLKLPLIDFSN+E G PKWEL +AQVKEALEEFGCFEASF+KVP+EVRK LFEAL
Sbjct: 1   MDGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDST+ +KVQ+FT ILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKG-PQSNETK 180
           TFSKVIE+YS+QLSELD++VRRMVLESLGVEKY++EHL STNYLLRVMKYKG  QSNETK
Sbjct: 121 TFSKVIETYSQQLSELDEMVRRMVLESLGVEKYMEEHLESTNYLLRVMKYKGTDQSNETK 180

Query: 181 LGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHS 240
           +GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WINF+PSPHSFVAMIGDS HAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPHSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAG 300
           PYHRVMMSG E RYSAGLFSIP+AGY +KAPEELVDE+HPLLF PFDHV+FLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QRAQSALKTYCGA 313
           Q+AQSALKT+CGA
Sbjct: 301 QKAQSALKTFCGA 313

[Close]

BLAST of Sgr030270 vs. ExPASy TrEMBL
Match: A0A6J1CQP5 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Momordica charantia OX=3673 GN=LOC111013275 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.0e-160
Identity = 277/312 (88.78%), Postives = 292/312 (93.59%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD ESSLKLPLIDFSN+E+GSPKWE+ +A VK+ALEEFGCFEASFEKV LE RK LFEAL
Sbjct: 1   MDCESSLKLPLIDFSNLELGSPKWEMAKALVKKALEEFGCFEASFEKVLLEARKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGV+DSTM EKVQ FT ILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVEDSTMLEKVQTFTNILWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFS+VIESYSKQLSELDQIVRRM+LESLGVEKYL+EHL STNYLLRVMKYKGPQS ETK+
Sbjct: 121 TFSQVIESYSKQLSELDQIVRRMILESLGVEKYLEEHLESTNYLLRVMKYKGPQSKETKI 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLHPHTDKN VTILYQN VEGLQIQ KDG WI+FKPSP SFVAMIGDSLHAWTNGRLHSP
Sbjct: 181 GLHPHTDKNFVTILYQNQVEGLQIQTKDGTWISFKPSPQSFVAMIGDSLHAWTNGRLHSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
            HRVMMSG EARYSAGLFS+P+AGY IKAP+ELVDEEHPLLFKPFDHVEFLQFYYT+AGQ
Sbjct: 241 RHRVMMSGKEARYSAGLFSVPKAGYIIKAPKELVDEEHPLLFKPFDHVEFLQFYYTQAGQ 300

Query: 301 RAQSALKTYCGA 313
            AQSALKTYCGA
Sbjct: 301 TAQSALKTYCGA 312

[Close]

BLAST of Sgr030270 vs. ExPASy TrEMBL
Match: A0A6J1F3R6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111441851 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.0e-155
Identity = 263/312 (84.29%), Postives = 291/312 (93.27%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD E SLKLP IDFSN+E GSPKWEL +A VKEAL+EFGCFEASF+KV  EVRK LFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDST+ EKVQ+F  I WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFSKVIESYS+QLSELD++VRRMV+ESLGVEKY++EHL STNYLLRVMKYKGP+SN+T+L
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESNDTQL 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WI+F+PSP+SFV MIGDS HAWTNGRL+SP
Sbjct: 181 GLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
           YHRV+MSG +ARYSAGLFSIP+AGY IKAP+ELVDEEHPLLF PFDHVEFLQFYYTEAGQ
Sbjct: 241 YHRVVMSGEKARYSAGLFSIPKAGYVIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAGQ 300

Query: 301 RAQSALKTYCGA 313
           RAQSALKTYCGA
Sbjct: 301 RAQSALKTYCGA 312

[Close]

BLAST of Sgr030270 vs. ExPASy TrEMBL
Match: A0A6J1J6J6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111482215 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.6e-155
Identity = 263/312 (84.29%), Postives = 291/312 (93.27%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEAL 60
           MD E SLKLP IDFSN+E GSPKWEL +A VKEAL+EFGCFEASF+KV  EVRK LFEAL
Sbjct: 1   MDSEPSLKLPHIDFSNLEAGSPKWELAKALVKEALQEFGCFEASFDKVADEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNP 120
           EELF+LPL+TKLRNVS+KPFHGYVGQYPMAPLFESMGVDDST+ EKVQ+F  I WPQGNP
Sbjct: 61  EELFDLPLQTKLRNVSKKPFHGYVGQYPMAPLFESMGVDDSTIPEKVQSFANIFWPQGNP 120

Query: 121 TFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKL 180
           TFSKVIESYS+QLSELD++VRRMV+ESLGVEKY++EHL STNYLLRVMKYKGP+SN+T+L
Sbjct: 121 TFSKVIESYSQQLSELDKMVRRMVVESLGVEKYMEEHLDSTNYLLRVMKYKGPESNDTQL 180

Query: 181 GLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSP 240
           GLH HTDKNIVTILYQNHVEGLQ+Q KDG+WI+F+PSP+SFV MIGDS HAWTNGRL+SP
Sbjct: 181 GLHSHTDKNIVTILYQNHVEGLQVQTKDGKWISFQPSPNSFVVMIGDSFHAWTNGRLYSP 240

Query: 241 YHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQ 300
           YHRV+MSG +ARYSAGLFSIP+AGY IKAP+ELVDEEHPLLF PFDHVEFLQFYYTEAGQ
Sbjct: 241 YHRVVMSGEKARYSAGLFSIPKAGYMIKAPKELVDEEHPLLFNPFDHVEFLQFYYTEAGQ 300

Query: 301 RAQSALKTYCGA 313
           RAQSALKTYCGA
Sbjct: 301 RAQSALKTYCGA 312

[Close]

BLAST of Sgr030270 vs. ExPASy TrEMBL
Match: A0A2R6QFZ5 (2-oxoglutarate-dependent dioxygenase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc17838 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.9e-141
Identity = 237/313 (75.72%), Postives = 276/313 (88.18%), Query Frame = 0


Query: 1   MDGESSLKLPLIDFSNIEI--GSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFE 60
           M  E+ L+LP IDFS  E+  G+P W+ V+AQV++ALEE+GCFEA F K+PL++ K LF 
Sbjct: 1   MGSETPLRLPTIDFSTPELNPGTPDWDSVKAQVRKALEEYGCFEAFFSKIPLDLLKALFG 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQG 120
           ALEELF+LPL+TKLRN S+KPFHGYVGQYPM PLFESMG+DD+ + EK + F+++LWP+ 
Sbjct: 61  ALEELFDLPLQTKLRNASKKPFHGYVGQYPMVPLFESMGIDDAPVPEKAKGFSQLLWPEE 120

Query: 121 NPTFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNET 180
           NP F K I+SYS+QLSELDQI+RRMVLESLGVEKYL EH+ STNYLLRVMKYKGPQ++ET
Sbjct: 121 NPNFYKTIQSYSEQLSELDQIIRRMVLESLGVEKYLDEHMESTNYLLRVMKYKGPQTSET 180

Query: 181 KLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLH 240
           KLGL+ HTDKNIVTILYQN VEGL++Q KDG WIN KPSPHSF+ MIGDS +AWTNGRLH
Sbjct: 181 KLGLNSHTDKNIVTILYQNQVEGLEVQTKDGEWINVKPSPHSFIVMIGDSFYAWTNGRLH 240

Query: 241 SPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEA 300
           SPYHRVMM+GNEARYSAGLFSIP+ GYTIKAP+ELVDE+HPLLFKPFDHV+FL FYYTEA
Sbjct: 241 SPYHRVMMTGNEARYSAGLFSIPKEGYTIKAPQELVDEDHPLLFKPFDHVQFLGFYYTEA 300

Query: 301 GQRAQSALKTYCG 312
           GQRAQSALK YCG
Sbjct: 301 GQRAQSALKAYCG 313

[Close]

BLAST of Sgr030270 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 3.5e-132
Identity = 216/307 (70.36%), Postives = 264/307 (85.99%), Query Frame = 0


Query: 7   LKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEELF 66
           L+LP+IDFS  N++ G P+W+L RA V++AL+++G FEASF+++P E+RK +F ALEELF
Sbjct: 10  LRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF 69

Query: 67  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNPTFSK 126
           +LPL+TKLRNVS+KPFHGYVGQYPM PL+ESMG+DDS ++EKV  FT+ LWPQGN +FS 
Sbjct: 70  DLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFST 129

Query: 127 VIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKLGLHP 186
            I+S+SK+LSELD  +RRM++ES G++KY+ EHL STNYLLRVMKYKGP + ETK+GL+ 
Sbjct: 130 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 189

Query: 187 HTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSPYHRV 246
           HTDKNIVTILYQNHVEGL++Q KD  WI  KP+  SF  MIGDSL+A  NGRLHSPYHRV
Sbjct: 190 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLYALLNGRLHSPYHRV 249

Query: 247 MMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQRAQS 306
           MM+G E RYS GLFSIP+AG+ + +P+ELVDEEHP LFKPFDHVEFLQFYYTEAGQR+QS
Sbjct: 250 MMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQS 309

Query: 307 ALKTYCG 312
           ALKTYCG
Sbjct: 310 ALKTYCG 316

[Close]

BLAST of Sgr030270 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 1.5e-98
Identity = 173/313 (55.27%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 6   SLKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEEL 65
           S +LP+IDFS  N++ GS KW+ V A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWP-QGNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + EKV +FT+ LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGP------QSN 185
           S+ I  +S+QL ELD +VRRM++ES G+E Y+ EHL ST YL R+MKY  P         
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKLGLHPHTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGR 245
           ETKLGL  HTDKNI+TIL+Q  V+GL+++ KD +WI  KPS  S + M+GDSL A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYT 305
           LHSPYHRV+M+G + RYS GLFSIP+ G  I +PEELVD+EHP +FKPF++ +FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQRAQSALKTY 310
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]

BLAST of Sgr030270 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.3e-80
Identity = 136/307 (44.30%), Postives = 201/307 (65.47%), Query Frame = 0


Query: 8   KLPLIDFSN---IEIGSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEALEELF 67
           K+P++D ++   ++  +  W  V  +  EALEE+GCF A ++ V  ++   +F A EELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNPTFSK 127
           +LP ETK +NV++KP+HGYVGQ P+ PL E +GVD  T  E  Q FT ++WPQGN  F  
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCN 128

Query: 128 VIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKLGLHP 187
            + ++S  ++ELD++V RM+ E+ GVEK+ + H+GS  YLL+ +KY  P  + +      
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFPQ 188

Query: 188 HTDKNIVTILYQNHVEGLQIQAKDGRWINFKPSPHSFVAMIGDSLHAWTNGRLHSPYHRV 247
           HTDK  ++IL+QN V GL++++KDG WI+ +  P S+V M GD    W+N R+ S  HRV
Sbjct: 189 HTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRSCEHRV 248

Query: 248 MMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQRAQS 307
            M G++ RY+ GLFS       +  PEELVD++HPL++KPFD++  + FY T+ G+ A S
Sbjct: 249 TMEGDKTRYTLGLFSF--LTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANS 308

Query: 308 ALKTYCG 312
            LK YCG
Sbjct: 309 TLKAYCG 313

[Close]

BLAST of Sgr030270 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.4e-79
Identity = 137/310 (44.19%), Postives = 209/310 (67.42%), Query Frame = 0


Query: 6   SLKLPLIDFS--NIEIGSPKWELVRAQVKEALEEFGCFEASF-EKVPLEVRKCLFEALEE 65
           S K+P +DFS  +++ G+  WE  R  +++ALEE+GCF     +K PL++   +F +L +
Sbjct: 2   SPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVD 61

Query: 66  LFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGNPTF 125
           LF+LP +TK++N   KP +GYVGQ P  PL ES+G+D++T  E  ++FT ++WPQGN  F
Sbjct: 62  LFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

Query: 126 SKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKGPQSNETKLGL 185
           S+ +  Y++  +ELDQ+V RMV +S  VEKY   ++ ST YLLRV+K + P +    LG 
Sbjct: 122 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 181

Query: 186 HPHTDKNIVTILYQNHVEGLQIQAKDGRWINFK-PSPHSFVAMIGDSLHAWTNGRLHSPY 245
             HTDK+  TIL+Q+ V GL+++ ++G  IN    SP  F+ + GD+L AW+N R+ SP 
Sbjct: 182 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 241

Query: 246 HRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFYYTEAGQR 305
           H+V++SG   RYS G+F+      T++ PEEL+D +HPL++KPFDH+  L+FY T+ G +
Sbjct: 242 HQVLVSGETDRYSLGMFAFNNG--TLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYK 301

Query: 306 AQSALKTYCG 312
           ++  +K YCG
Sbjct: 302 SECPIKAYCG 309

[Close]

BLAST of Sgr030270 vs. TAIR 10
Match: AT1G28030.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 145/316 (45.89%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 2   DGESSLKLPLIDFSNIEI--GSPKWELVRAQVKEALEEFGCFEASFEKVPLEVRKCLFEA 61
           + +  L LP+IDFSN ++   +P+W+LVR+QV++ALEE+GCFEA F+   +E+RK LFE+
Sbjct: 6   ESQVPLSLPIIDFSNPDLKPETPEWDLVRSQVRKALEEYGCFEALFDGASMELRKALFES 65

Query: 62  LEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTMSEKVQNFTKILWPQGN 121
            +E+F+LPLETKL   +   + GY+   P  P+ E MG         V + T  LWPQGN
Sbjct: 66  SKEVFDLPLETKLSTKTDVHYEGYL-TIPRVPIQEGMGFYGIDNPNVVNDLTHKLWPQGN 125

Query: 122 PTFSKVIESYSKQLSELDQIVRRMVLESLGVEKYLKEHLGSTNYLLRVMKYKG--PQSNE 181
               K ++S++++L EL+  VR M LES G+EKY++EHL + N   +++KYKG    + E
Sbjct: 126 IFVGKNVQSFAEKLIELNLTVRTMTLESFGLEKYMEEHLNAANKHFQLLKYKGISDDNTE 185

Query: 182 TKLGLHPHTDKNIVTILYQNH-VEGLQIQAKDG-RWINFKPS-PHSFVAMIGDSLHAWTN 241
            K+G +PH D++ +TIL QN  V+GL+I+ KDG  WI  KPS   SF+ M G SLH   N
Sbjct: 186 NKIGFYPHIDRHFLTILCQNDAVDGLEIKTKDGEEWIKVKPSQASSFIVMAGASLHVLLN 245

Query: 242 GRLHSPYHRVMMSGNEARYSAGLFSIPRAGYTIKAPEELVDEEHPLLFKPFDHVEFLQFY 301
           G +  P HRV+++G + RY A LF+IP+ G  I APEE+VD+EHP L+KPFD   FL+F 
Sbjct: 246 GGVFPPLHRVVITGKKDRYVAALFTIPKEGVIINAPEEMVDDEHPRLYKPFDFWGFLKFS 305

Query: 302 YTEAGQRAQSALKTYC 311
                ++  S L  YC
Sbjct: 306 NLPNARKDISDLTDYC 320

The following BLAST results are available for this feature:

BLAST of Sgr030270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906019.1	6.1e-163	88.78	probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida]	[more]
XP_008454372.1	2.0e-161	87.54	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo]	[more]
XP_022143397.1	2.2e-160	88.78	probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Momordica charantia]	[more]
XP_004152262.1	2.0e-158	85.94	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8649036...	[more]
XP_023528402.1	2.7e-155	84.94	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr030270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	9.3e-98	55.45	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	2.1e-97	55.27	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	9.6e-95	55.63	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q9ZTA2	5.5e-74	37.37	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]
Q945B5	2.7e-73	35.43	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Sgr030270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BYJ6	9.5e-162	87.54	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1CQP5	1.0e-160	88.78	probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Momordica charantia OX=3...	[more]
A0A6J1F3R6	5.0e-155	84.29	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1J6J6	6.6e-155	84.29	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A2R6QFZ5	1.9e-141	75.72	2-oxoglutarate-dependent dioxygenase OS=Actinidia chinensis var. chinensis OX=15...	[more]

BLAST of Sgr030270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52820.1	3.5e-132	70.36	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.5e-98	55.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	5.3e-80	44.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	3.4e-79	44.19	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G28030.1	3.9e-75	45.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 221..247 score: 29.63 coord: 185..206 score: 38.84
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..311
None	No IPR available	PANTHER	PTHR47990:SF128	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 5..311
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 5..298
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 7..310 e-value: 3.2E-69 score: 235.6
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 9..85 e-value: 3.6E-12 score: 47.1
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 167..258 e-value: 4.5E-21 score: 75.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 160..261 score: 11.325882

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr030270.1	Sgr030270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0002238	response to molecule of fungal origin
molecular_function	GO:0016706	2-oxoglutarate-dependent dioxygenase activity
molecular_function	GO:0046872	metal ion binding