Sgr030253 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030253
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionProtein-lysine N-methyltransferase LOC111013281
Locationtig00153640: 1467307 .. 1469902 (-)
RNA-Seq ExpressionSgr030253
SyntenySgr030253
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCGATTCGATTGACGCCGGAAGACGCTGAGCTGTTGCAGCAGGCTCGAGCAGCTCCGTCCGCCGTCGATCTGGTCTCCGATGACGACCGCTCAGTGGCCGCTGACTCATGGTCCATCAAGAGCGAGTATGGTAGCACCCTTGACGATGACCAGCGTAATGCCGACGCTGCTGAGGCTCTTTCTGCCGGGAATTTGCGTGCCGCCTCCGATTACAGGTATAGCTTACTTTCTTCTTTCGAATTATCTGAATCTAACCAGAAGTAGTAATTGAAACTCATTTCTTTTAGAACTTTGTTATTGTTTGGCAGCTGGGGCTTTGGGAATTTGGACATTTCTAGATTACTTCTTTATTTGGGGTTTTCTTTTTTAGTTCAGACTGATTCCTAAAGCATTCAGTTATCTTGTTGTTTTCGTTGATTTATGGAATAAATGAATATGAGTTAGCTGAAAAGTTCTGCGGCGTAGTTCGCGTAGTTTACCTTTTATCTTACACTTATATTGAACTCCTAAAGGTGTGAGTACAATCTCATTTCTTCAGTCTCGTGCTCTTTCTTCCTTTAGTCCTATGGACTACCTTTTTTCTGTCTAGGTGTCGTGTTTGCTTAGAGATTAGATCTATGTTCCCCTACGTTTCGCTGTAATTAATGTCACAACTCACAAGCATTGCTTAGACACATTGTAACGCACAGGCATGTAGAATGTTCCATCTGTAACTTACGTGACCAAATTGAAAAATAATAGTACTATTAATCTGGTACAATGGGATTTTTTTTCCTGGGCGTATTTTCATGTTTTCATAAGACCTGTTTCACTTTCAGTTCTGACAAGGATGAAATGGAACCGGATGCTGAGGCTGTGACATCAATGCTAGGTCTCCAAACTTATTGGGATTCTCAATATGCTGATGAATTGGCAAATTTTCGTGAACATGGGCATGTTGGTGAGGTCTGGTATGTAAGAATTGCTTAAGGTATCTTATTTCCTAATATTCTTTGTAAGTGATATCGTAATGTTATTGTTGGCAATCAGCCACAAGGTGTAGTTAGTAGTTAGCTGCACTTCAATTATCTCTACTTTTTTGGCCTTTTGTATTATTCAACTACTGATCCTATTCTCTTATCACTTTTCTTTGTCAATGTACCATATGTCGGTACTCTGTGTACTACTGTTAATTTGGTTCCTTTGAGAGATTTAAGAACTGTTCTTGGACTCTTTAACTTTCTGAACAAGAATATTAGATATTAGTCATTGAAAGGATAATGCTACTTGAAAATTATTGTCTATAATTTATCTTGAGAAGATATTAGTCATTCAAATGTTGTTAAACTTTATTTACTAGTTTAACTTTACTGACTAAAAAATAAAATAGATTTCTTCTAGATTGCGGGGAAGAATCAGAGAAAGACTTAACTTGTATTTGTTTATCGAGTTTTCTATTTTTCTTTCTTCTTTCTTCAGGTTTGGATCTGAAGTTATGGAAACTGTTGCATCTTGGACAAAAAGCTTGTGTTCTGATGTTTCTCAAGGTCGGTTTCCAAATCAAGCTGATAATGTTAAGCCACTGAATGTTGAACAAGGTTACAAATTCTTGACTAGTTGGAATGTTCTTGACATTGGGACGGGCAATGGTTTGCTGCTTCAAGAACTTGCTAAGCAGGGGTATGTTTTGAGATTTTGCATTTTGTTTTTTGTCAACATTTAAATGGAACTTTTGTAACAATTTTTGTTTTTTGACATATATCTTAATTCAAGTGGAGGATAAGGTTTATTGTAATAGATGCACCAATTGAAGGAACAAGCTAGTTGGTTGATAAATTGAGTAGGATAACTATATCCTTTTAAAAAAAGGGAAAAAAAATACAAGGGCAAAAAAATACCCTTTTATTTCCTTAGTTTTGAGTTTAGTTTCCATTTGGTCCCTAGGTTTCAAAATCAAACACTTTTGTCCTTGAAGTTTCAATAATGCTCCCTTTTGGTCCTTGAGATAATAGAACCGTTAGTAACCCTAACGCAATGCTGCCTAAGATGCTGACTGGGCCCATTTAATATTAATCTATATTATTTTATGCTGATGTGGCACAAATATTTATTTTTATAATATTTTATGCGTGATGTGGCGAAATGTTTCTCTCTCTTTTCCCTCTTCCCCTACACCCGGCCCCTTTCCCCTACCCTTGCCATCGGCCACCCCCTTCCCCTACCCTCGCCTCTAGCCTCTCCCCCTTCCCTCGTCCCCGCATGACCCTCTACAACCTTTGGCCCCCCCACCCTTTCCTCGCCTTGCCACATTCCAGCACAACACCCCTACCCCCGTGCCCCCACCATGCTCTCTCTCTCCCTTATTTTTCTCTCGTCCCCACCCACGCTGCCTAGACACTTGCCCTCGCAGCCTTTTCGCCTTTAATCTGAAAATTTTCAGATCTGGAAAATCTTTGATTCACAGAGAGGGGGTGTGGTGGGCGACGAGATGATGGGGTGGGGAAGAGAGAGGGGGGGGGGGCGACAAGATGGTGGGGTGGGGGCTCGAGTGGCTGGCGACAAAGGAAGGGGAAAAGGGAAGAGGGGGTGGGGGATGGGGATGGTTGGGTAG

mRNA sequence

ATGGCCGCGATTCGATTGACGCCGGAAGACGCTGAGCTGTTGCAGCAGGCTCGAGCAGCTCCGTCCGCCGTCGATCTGGTCTCCGATGACGACCGCTCAGTGGCCGCTGACTCATGGTCCATCAAGAGCGAGTATGGTAGCACCCTTGACGATGACCAGCGTAATGCCGACGCTGCTGAGGCTCTTTCTGCCGGGAATTTGCGTGCCGCCTCCGATTACAGTTCTGACAAGGATGAAATGGAACCGGATGCTGAGGCTGTGACATCAATGCTAGGTCTCCAAACTTATTGGGATTCTCAATATGCTGATGAATTGGCAAATTTTCGTGAACATGGGCATGTTGGTGAGGTCTGGTTTGGATCTGAAGTTATGGAAACTGTTGCATCTTGGACAAAAAGCTTGTGTTCTGATGTTTCTCAAGGTCGGTTTCCAAATCAAGCTGATAATGTTAAGCCACTGAATGTTGAACAAGGTTACAAATTCTTGACTAGTTGGAATGTTCTTGACATTGGGACGGGCAATGGTTTGCTGCTTCAAGAACTTGCTAAGCAGGGAGGGGGTGTGGTGGGCGACGAGATGATGGGGTGGGGAAGAGAGAGGGGGGGGGGGCGACAAGATGGTGGGGTGGGGGCTCGAGTGGCTGGCGACAAAGGAAGGGGAAAAGGGAAGAGGGGGTGGGGGATGGGGATGGTTGGGTAG

Coding sequence (CDS)

ATGGCCGCGATTCGATTGACGCCGGAAGACGCTGAGCTGTTGCAGCAGGCTCGAGCAGCTCCGTCCGCCGTCGATCTGGTCTCCGATGACGACCGCTCAGTGGCCGCTGACTCATGGTCCATCAAGAGCGAGTATGGTAGCACCCTTGACGATGACCAGCGTAATGCCGACGCTGCTGAGGCTCTTTCTGCCGGGAATTTGCGTGCCGCCTCCGATTACAGTTCTGACAAGGATGAAATGGAACCGGATGCTGAGGCTGTGACATCAATGCTAGGTCTCCAAACTTATTGGGATTCTCAATATGCTGATGAATTGGCAAATTTTCGTGAACATGGGCATGTTGGTGAGGTCTGGTTTGGATCTGAAGTTATGGAAACTGTTGCATCTTGGACAAAAAGCTTGTGTTCTGATGTTTCTCAAGGTCGGTTTCCAAATCAAGCTGATAATGTTAAGCCACTGAATGTTGAACAAGGTTACAAATTCTTGACTAGTTGGAATGTTCTTGACATTGGGACGGGCAATGGTTTGCTGCTTCAAGAACTTGCTAAGCAGGGAGGGGGTGTGGTGGGCGACGAGATGATGGGGTGGGGAAGAGAGAGGGGGGGGGGGCGACAAGATGGTGGGGTGGGGGCTCGAGTGGCTGGCGACAAAGGAAGGGGAAAAGGGAAGAGGGGGTGGGGGATGGGGATGGTTGGGTAG

Protein sequence

MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFGSEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQELAKQGGGVVGDEMMGWGRERGGGRQDGGVGARVAGDKGRGKGKRGWGMGMVG
Homology
BLAST of Sgr030253 vs. NCBI nr
Match: XP_038896924.1 (EEF1A lysine methyltransferase 2 [Benincasa hispida])

HSP 1 Score: 347.4 bits (890), Expect = 9.3e-92
Identity = 175/185 (94.59%), Postives = 180/185 (97.30%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
            EVMETVASWTKSLC DVSQGRFPNQADNVK LNV+QG KFL+SW+VLDIGTGNGLLLQE
Sbjct: 121 PEVMETVASWTKSLCCDVSQGRFPNQADNVKSLNVDQGSKFLSSWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
           LAK+G
Sbjct: 181 LAKEG 185

BLAST of Sgr030253 vs. NCBI nr
Match: XP_022143402.1 (EEF1A lysine methyltransferase 2 [Momordica charantia])

HSP 1 Score: 344.4 bits (882), Expect = 7.9e-91
Identity = 173/185 (93.51%), Postives = 180/185 (97.30%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELL QARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAVRLTPEDAELLHQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADELANFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELANFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           SEVMETVASWTKSLCSDVSQG+ PNQ+DNVK L+VEQG KFLTSW+VLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCSDVSQGQIPNQSDNVKSLDVEQGSKFLTSWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
           LAK+G
Sbjct: 181 LAKEG 185

BLAST of Sgr030253 vs. NCBI nr
Match: XP_004140734.1 (EEF1A lysine methyltransferase 2 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 172/185 (92.97%), Postives = 178/185 (96.22%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAVRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           SEVMETVASWTKSLC DVSQGRF NQA NVK LNV+QG KFL+SW+VLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCYDVSQGRFLNQAGNVKTLNVDQGSKFLSSWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
           LAK+G
Sbjct: 181 LAKEG 185

BLAST of Sgr030253 vs. NCBI nr
Match: XP_008457104.1 (PREDICTED: protein-lysine N-methyltransferase Mettl10 [Cucumis melo])

HSP 1 Score: 336.3 bits (861), Expect = 2.1e-88
Identity = 170/185 (91.89%), Postives = 175/185 (94.59%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSA DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 54  MAAVRLTPEDAELLQQARAAPSAADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 113

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 114 ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 173

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           S VMETVASWTKSLC DVSQGRFPNQA NVK LNV+QG  FL+SW VLDIGTGNGLLLQE
Sbjct: 174 SGVMETVASWTKSLCYDVSQGRFPNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQE 233

Query: 181 LAKQG 186
           LAK+G
Sbjct: 234 LAKEG 238

BLAST of Sgr030253 vs. NCBI nr
Match: KAA0060716.1 (protein-lysine N-methyltransferase Mettl10 [Cucumis melo var. makuwa] >TYK01459.1 protein-lysine N-methyltransferase Mettl10 [Cucumis melo var. makuwa])

HSP 1 Score: 336.3 bits (861), Expect = 2.1e-88
Identity = 170/185 (91.89%), Postives = 175/185 (94.59%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSA DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 54  MAAVRLTPEDAELLQQARAAPSAADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 113

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 114 ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 173

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           S VMETVASWTKSLC DVSQGRFPNQA NVK LNV+QG  FL+SW VLDIGTGNGLLLQE
Sbjct: 174 SGVMETVASWTKSLCYDVSQGRFPNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQE 233

Query: 181 LAKQG 186
           LAK+G
Sbjct: 234 LAKEG 238

BLAST of Sgr030253 vs. ExPASy Swiss-Prot
Match: Q5JPI9 (EEF1A lysine methyltransferase 2 OS=Homo sapiens OX=9606 GN=EEF1AKMT2 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 44/117 (37.61%), Postives = 53/117 (45.30%), Query Frame = 0

Query: 69  AASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFGSEVMETVA 128
           AA    SDK     D   V S LG + +WD+ Y  EL  FRE+G  GE+WFG E M  + 
Sbjct: 11  AAVAARSDKGSPGEDG-FVPSALGTREHWDAVYERELQTFREYGDTGEIWFGEESMNRLI 70

Query: 129 SWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQELAKQG 186
            W                          Q +K     +VLDIGTGNG+ L ELAK G
Sbjct: 71  RWM-------------------------QKHKIPLDASVLDIGTGNGVFLVELAKFG 101

BLAST of Sgr030253 vs. ExPASy Swiss-Prot
Match: Q5D013 (EEF1A lysine methyltransferase 2 OS=Danio rerio OX=7955 GN=eef1akmt2 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 35/97 (36.08%), Postives = 47/97 (48.45%), Query Frame = 0

Query: 89  SMLGLQTYWDSQYADELANFREHGHVGEVWFGSEVMETVASWTKSLCSDVSQGRFPNQAD 148
           S LG + YWD  Y  EL  +++ G VGE+WFG E M  V  W ++           N ++
Sbjct: 26  SKLGTKEYWDGAYKRELQTYKDIGDVGEIWFGEESMHRVIRWMEA----------QNISE 85

Query: 149 NVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQELAKQG 186
           N                 +LDIGTGNG+ L ELA+ G
Sbjct: 86  NAA---------------ILDIGTGNGMFLVELARHG 97

BLAST of Sgr030253 vs. ExPASy Swiss-Prot
Match: Q9D853 (EEF1A lysine methyltransferase 2 OS=Mus musculus OX=10090 GN=Eef1akmt2 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 44/131 (33.59%), Postives = 57/131 (43.51%), Query Frame = 0

Query: 55  NADAAEALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHV 114
           NADA     A     + + SS  D+  P A      LG + +WD+ Y  EL  F+E+G  
Sbjct: 2   NADAEGHSGAVVPAQSPEGSSAADDFVPSA------LGTREHWDAVYERELRTFQEYGDT 61

Query: 115 GEVWFGSEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGN 174
           GE+WFG E M  +  W                          Q +K     +VLDIGTGN
Sbjct: 62  GEIWFGEESMNRLIRWM-------------------------QKHKIPLDASVLDIGTGN 101

Query: 175 GLLLQELAKQG 186
           G+ L EL K G
Sbjct: 122 GVFLVELVKHG 101

BLAST of Sgr030253 vs. ExPASy Swiss-Prot
Match: P40516 (Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EFM4 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.6e-06
Identity = 35/104 (33.65%), Postives = 48/104 (46.15%), Query Frame = 0

Query: 79  EMEPDAEAVTSMLGLQTYWDSQYADELANFREHGH-VGEVWFGSEVMETVASWTKSLCSD 138
           +M+  A+  TS LG + YWD  YA EL NFR +    G+ WF                SD
Sbjct: 28  QMQGTADLSTSKLGTKKYWDELYALELENFRRNPQDTGDCWFSD--------------SD 87

Query: 139 VSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQEL 182
             Q       DN+        Y+   + +V+D+GTGNG +L EL
Sbjct: 88  AEQKMIDFLVDNI------GAYRISENASVVDLGTGNGHMLFEL 111

BLAST of Sgr030253 vs. ExPASy TrEMBL
Match: A0A6J1CQQ0 (Protein-lysine N-methyltransferase LOC111013281 OS=Momordica charantia OX=3673 GN=LOC111013281 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.8e-91
Identity = 173/185 (93.51%), Postives = 180/185 (97.30%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELL QARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAVRLTPEDAELLHQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADELANFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELANFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           SEVMETVASWTKSLCSDVSQG+ PNQ+DNVK L+VEQG KFLTSW+VLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCSDVSQGQIPNQSDNVKSLDVEQGSKFLTSWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
           LAK+G
Sbjct: 181 LAKEG 185

BLAST of Sgr030253 vs. ExPASy TrEMBL
Match: A0A0A0L6T8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G187230 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 7.2e-90
Identity = 172/185 (92.97%), Postives = 178/185 (96.22%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAVRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           SEVMETVASWTKSLC DVSQGRF NQA NVK LNV+QG KFL+SW+VLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCYDVSQGRFLNQAGNVKTLNVDQGSKFLSSWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
           LAK+G
Sbjct: 181 LAKEG 185

BLAST of Sgr030253 vs. ExPASy TrEMBL
Match: A0A5D3BTP8 (Protein-lysine N-methyltransferase E5676_scaffold451G00020 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00020 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.0e-88
Identity = 170/185 (91.89%), Postives = 175/185 (94.59%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSA DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 54  MAAVRLTPEDAELLQQARAAPSAADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 113

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 114 ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 173

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           S VMETVASWTKSLC DVSQGRFPNQA NVK LNV+QG  FL+SW VLDIGTGNGLLLQE
Sbjct: 174 SGVMETVASWTKSLCYDVSQGRFPNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQE 233

Query: 181 LAKQG 186
           LAK+G
Sbjct: 234 LAKEG 238

BLAST of Sgr030253 vs. ExPASy TrEMBL
Match: A0A1S3C4B8 (Protein-lysine N-methyltransferase LOC103496859 OS=Cucumis melo OX=3656 GN=LOC103496859 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.0e-88
Identity = 170/185 (91.89%), Postives = 175/185 (94.59%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAA+RLTPEDAELLQQARAAPSA DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 54  MAAVRLTPEDAELLQQARAAPSAADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 113

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLR ASDYSSDKDEMEPDAEAVTSMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 114 ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 173

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           S VMETVASWTKSLC DVSQGRFPNQA NVK LNV+QG  FL+SW VLDIGTGNGLLLQE
Sbjct: 174 SGVMETVASWTKSLCYDVSQGRFPNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQE 233

Query: 181 LAKQG 186
           LAK+G
Sbjct: 234 LAKEG 238

BLAST of Sgr030253 vs. ExPASy TrEMBL
Match: A0A6J1KBC4 (Protein-lysine N-methyltransferase LOC111492750 OS=Cucurbita maxima OX=3661 GN=LOC111492750 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.1e-87
Identity = 167/185 (90.27%), Postives = 174/185 (94.05%), Query Frame = 0

Query: 1   MAAIRLTPEDAELLQQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60
           MAAIRLTPEDAELL QARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAIRLTPEDAELLHQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 61  ALSAGNLRAASDYSSDKDEMEPDAEAVTSMLGLQTYWDSQYADELANFREHGHVGEVWFG 120
           ALSAGNLRAASDYSSDKDE+E D EAV+SMLGLQ+YWDSQYADEL NFREHGHVGEVWFG
Sbjct: 61  ALSAGNLRAASDYSSDKDEIEADTEAVSSMLGLQSYWDSQYADELTNFREHGHVGEVWFG 120

Query: 121 SEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGTGNGLLLQE 180
           SEVMETVASWTKSLCSDVSQG+FPNQADN K  NVEQ +KFL  W+VLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCSDVSQGQFPNQADNAKTQNVEQDFKFLDGWSVLDIGTGNGLLLQE 180

Query: 181 LAKQG 186
            AK+G
Sbjct: 181 FAKEG 185

BLAST of Sgr030253 vs. TAIR 10
Match: AT1G66680.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 7.7e-60
Identity = 122/193 (63.21%), Postives = 144/193 (74.61%), Query Frame = 0

Query: 1   MAAIRLTPEDAELL--QQARAAPSAV-----DLVSDDDRSVAADSWSIKSEYGSTLDDDQ 60
           MA IRL PE+ E    QQARAA +        L SDDDRS+AADSWSIKSEYGSTLDDDQ
Sbjct: 1   MAGIRLLPEEPETTPQQQARAAAAVTTTTTDSLASDDDRSIAADSWSIKSEYGSTLDDDQ 60

Query: 61  RNADAAEALSAGNLRAASDYSSDKDEMEPDAE-AVTSMLGLQTYWDSQYADELANFREHG 120
           R+ADAAEALS+ N R +SDYSSDK+  EPDA+    SMLGLQ+YWD+ Y+DEL NFREHG
Sbjct: 61  RHADAAEALSSANFRVSSDYSSDKE--EPDADGGGQSMLGLQSYWDAAYSDELTNFREHG 120

Query: 121 HVGEVWFGSEVMETVASWTKSLCSDVSQGRFPNQADNVKPLNVEQGYKFLTSWNVLDIGT 180
           H GEVWFG +VME V SWTK LC ++SQ       ++V     +Q  K+L+SWNVLD+GT
Sbjct: 121 HAGEVWFGDDVMEIVTSWTKDLCVEISQRNMSVSENDVTTEVNDQADKYLSSWNVLDLGT 180

Query: 181 GNGLLLQELAKQG 186
           GNGLLL +LAK+G
Sbjct: 181 GNGLLLHQLAKEG 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896924.19.3e-9294.59EEF1A lysine methyltransferase 2 [Benincasa hispida][more]
XP_022143402.17.9e-9193.51EEF1A lysine methyltransferase 2 [Momordica charantia][more]
XP_004140734.11.5e-8992.97EEF1A lysine methyltransferase 2 [Cucumis sativus][more]
XP_008457104.12.1e-8891.89PREDICTED: protein-lysine N-methyltransferase Mettl10 [Cucumis melo][more]
KAA0060716.12.1e-8891.89protein-lysine N-methyltransferase Mettl10 [Cucumis melo var. makuwa] >TYK01459.... [more]
Match NameE-valueIdentityDescription
Q5JPI93.2e-1037.61EEF1A lysine methyltransferase 2 OS=Homo sapiens OX=9606 GN=EEF1AKMT2 PE=1 SV=2[more]
Q5D0131.6e-0936.08EEF1A lysine methyltransferase 2 OS=Danio rerio OX=7955 GN=eef1akmt2 PE=2 SV=1[more]
Q9D8531.6e-0933.59EEF1A lysine methyltransferase 2 OS=Mus musculus OX=10090 GN=Eef1akmt2 PE=1 SV=1[more]
P405163.6e-0633.65Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A6J1CQQ03.8e-9193.51Protein-lysine N-methyltransferase LOC111013281 OS=Momordica charantia OX=3673 G... [more]
A0A0A0L6T87.2e-9092.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G187230 PE=4 SV=1[more]
A0A5D3BTP81.0e-8891.89Protein-lysine N-methyltransferase E5676_scaffold451G00020 OS=Cucumis melo var. ... [more]
A0A1S3C4B81.0e-8891.89Protein-lysine N-methyltransferase LOC103496859 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1KBC41.1e-8790.27Protein-lysine N-methyltransferase LOC111492750 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G66680.17.7e-6063.21S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 91..193
e-value: 2.8E-6
score: 28.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..232
NoneNo IPR availablePANTHERPTHR12843PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10coord: 1..186
NoneNo IPR availablePANTHERPTHR12843:SF12PROTEIN-LYSINE N-METHYLTRANSFERASE 102587567coord: 1..186
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 93..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030253.1Sgr030253.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018022 peptidyl-lysine methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016279 protein-lysine N-methyltransferase activity