Sgr030035 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030035
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPectinesterase
Locationtig00153554: 2332388 .. 2336003 (-)
RNA-Seq ExpressionSgr030035
SyntenySgr030035
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCTCTTTCAATGGAGAGGAGTCTTGGGGTGTTGTTTTTTCTTCAGTTCTTTCTACTATCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACATACCCTGAAACCTGCAAGTATTTCTTCAACCATGGCAGCAAGGCTGCTCCTAAATATATGTCTGAGTTCAAGAAAATGGCGATCCAATTCGCCATGGAGAGAGCTCTGAGTGCCGAGAGCCACACCAAGGGTGTCGGTTTGAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATTCTTCAGCTCAACCACACTTTAGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACGGCCCTAACCAACCTTGAAACTTGCCGCGCCGGGTTTGCTGAGCTTAATGTTTCAGACTATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCAGGGAAAGAGACATATAAAGAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGACAGGAGGCTACTGCAGTCATCTACAACAAATGCGAACCTTGTCGTGGCTCAGGATGGATCCGGTGACCATAAGACGGTTGGGGCGGCTCTAGAGGCAGCTGCAAAAGGGAAGACAGATGGGAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAGAACCTTGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTAGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCAGCTACTGTTGGTAAGTCCCCCTCTCTCTCTCTCTCTCTCTCTCTTTCTAGGTTGACTGCTTATAAGGTCATTTACCTCCATTTCAACAACATTTGAATCTGTCAACCAGCATATTTTACCCATTCCAAGTCCAGCAACTCCATTGATAGATTTGTCATCTAGAAAGTAAACACTAGACAAAAGTTCTTGCACCCCAAGATATAGTGCTTAGAATTTTCTCTTACTCTCCATTTCCAAACAAAAATATAATCAGCCTCTTGGTCAAGATCAATACAAAAAATGGAAAAAAATTCTATGGCATTTCATCATAAATGCATCATTTATAAGATCAAATGGCTGTCAAGTGTCAATTAATAACCTCAATAATGTGAAAAGTATACGATTAATGTGAGATACTTACTTGTGTTCTGTGACAATCAGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCGTAACACAGCTGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCTGGTGCTGATCTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGACACCTCTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACAGTGGACTTTATATTCGGCAATGCTGCAGTTGTTCTACAAAACTGCATGATTTATGCGAGGAGGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACTGATCCAAACCAAAATACAGGCATTTCAATCCACAACTCACGGGTCATGGCCACAGATGACCTTAAACCAGTGGAGAGCACAGTGAGAACCTACTTGGGAAGGCCGTGGAAGGAGTTCTCTCGAACAGTTTTCATGCAAACTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAGTGGGATGGTGACTTTGCTCTGAATACTTTATATTATGGAGAGTATAAGAACAATGGTCCTCGTTCGTCAACCAGCCAGAGAGTGAAGTGGAAGGGTTACCATGCTATAAGCAATTCAACAGAAGTATCGAAGTTCACTGTCCAAAATTTCATCGCAGGACAGTCATGGTTGCCAGGCACAGAGGTCCCATTTAACCCTGGACTCTAAAAGAAAAGATTTTTCTTCATTCTTCCTGCGAAATAAACATAGCCCAAATCATTTAATTCTTTAATGTGCATGCAGTTTTAAAATTCATCAGTCAAGCTCATATTTCAAATGAAAAGTTACATTAATAAGCTAGAATGGACAAACTTGATGGACAAGGGAATTGGAGATGAGATTCCGAACTATGGAACAAACATGTTACTGAGACTAGAAATCAACTAGAAAAGATGTGGTCTCTAAATTTTGTTCATGTTTCTTAATCCAAAGAGTTGACAGAGGACTCCAGGACGATTTCCAAATTTTTGATCTGCCTTAACTCTGTTCATCCTGACACGTACACTAATAGCCCAAATGAAAACAAGATTGAAGAATCATAGAAGCAAAGATTCATTCTCTCTTTATTTCAAACACTGATGATCTGAGGACCAGCACTCTCAACCTCAACCGGGTAGTTCTTAGAATTAGCAGAGGGGCGATGGTCAGCCAAAGCATCTTCCTGAAACCTGATACAATTTCCAAACCCAAGCTTGTAGCCAATCAACTTCTCCCAAAATTTATAGCATTTTTAGTACCAAGGCCCTATTGTACCCCTTGGAAGAAATTTTTGTGCACCGCTCATGAGTATCATCCAACCCATAAGTACGGATGATAACTCTCCTCCATAAAGAATCCTCTTCTGGGTTGATCACAAGCAACCTCTTTTATGTTTCCAATACAGAGACACTCCCACATCACGAGAAAGAGAGAGACAGCTACACTTAGACCGACACCCACCATCTAAATTGGCTCCTTCCTTCCTATACAGTCTAATAATTTTCTAGAAAGCCTCCAACCACTTTTTAGAGTTAATCTTAAAGAGAGACAGGTGATAAGTTGGTAATAAGTTGGTAAACTGGGAACAATTGATTCTACCTATGTTATCTTTCCCTCCTTAGAGGTAGAGATATTTTTCACATTTCTCCAATCAGATTTACATATGCTAGAAGGAAATCGGCCAAGTCTAAATAATAGGATTTAGGATGGCGCTCTCCCCGCCCTCAGTCCCTACACCCATGGCGGCCTGCTCTTCCCCGTCGCCATCCTCCTAACCTGAAACATGAAGGACTGGGCCTGCCCAGACCAATCCTTCTCCATTGACAGAAAAACTTTCACCATTTTCTTTGACAGAGTCTACTGAGGAAGCAGGGCTAGAATCTCAGAAATCGGGAATACACCTACCGATTCGCTTCAATTCAATTCACTTTTTTCAATACTCGATTCGGTTATGGGACCAATCCTAATCAATTCGGCTGGTTTGATTTTAAAAATAAAAAATATAATAAAAAACGATTTTAAGAATACATAATTGAGGGGAACGAAAAAGGGTGGCTGTCGTGTTGAGAGCTTCCCAGGAGGCACAATTGGGCTTGAAAGAACCGCCCGTTATCCTTCCACTGACGGGAGTTGGAGGTGACGCTGCTCTGTGAAGCCAGCCTCACGCGTTTCCCTCCAATCTAAGAACAGAGACACCTGGGCCTTCGCCCAGAACGCAAAGCTCAACTCCAGTCTTGAAATGGGCAAGCCAGGAAACCTCTTAACAAAGACCCCGCCTAATAGGAAGAAACAACCTTCAACCACTTTGGCGCCCCCACTCGGGTAA

mRNA sequence

ATGACTGCTCTTTCAATGGAGAGGAGTCTTGGGGTGTTGTTTTTTCTTCAGTTCTTTCTACTATCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACATACCCTGAAACCTGCAAGTATTTCTTCAACCATGGCAGCAAGGCTGCTCCTAAATATATGTCTGAGTTCAAGAAAATGGCGATCCAATTCGCCATGGAGAGAGCTCTGAGTGCCGAGAGCCACACCAAGGGTGTCGGTTTGAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATTCTTCAGCTCAACCACACTTTAGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACGGCCCTAACCAACCTTGAAACTTGCCGCGCCGGGTTTGCTGAGCTTAATGTTTCAGACTATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCAGGGAAAGAGACATATAAAGAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGACAGGAGGCTACTGCAGTCATCTACAACAAATGCGAACCTTGTCGTGGCTCAGGATGGATCCGGTGACCATAAGACGGTTGGGGCGGCTCTAGAGGCAGCTGCAAAAGGGAAGACAGATGGGAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAGAACCTTGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTAGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCAGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCGTAACACAGCTGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCTGGTGCTGATCTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGACACCTCTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACAGAGGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACTGATCCAAACCAAAATACAGGCATTTCAATCCACAACTCACGGGTCATGGCCACAGATGACCTTAAACCAGTGGAGAGCACAGTGAGAACCTACTTGGGAAGGCCGTGGAAGGAGTTCTCTCGAACAGTTTTCATGCAAACTACCTCGATAGTTTGGTTGATCCAGCAGTATAAGAACAATGGTCCTCGTTCGTCAACCAGCCAGAGAGTGAAGTGGAAGGGTTACCATGCTATAAGCAATTCAACAGAAGTATCGAAGTTCACTGTCCAAAATTTCATCGCAGGACAGTCATGGTTGCCAGGCACAGAGCCAGCCTCACGCGTTTCCCTCCAATCTAAGAACAGAGACACCTGGGCCTTCGCCCAGAACGCAAAGCTCAACTCCAGTCTTGAAATGGGCAAGCCAGGAAACCTCTTAACAAAGACCCCGCCTAATAGGAAGAAACAACCTTCAACCACTTTGGCGCCCCCACTCGGGTAA

Coding sequence (CDS)

ATGACTGCTCTTTCAATGGAGAGGAGTCTTGGGGTGTTGTTTTTTCTTCAGTTCTTTCTACTATCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACATACCCTGAAACCTGCAAGTATTTCTTCAACCATGGCAGCAAGGCTGCTCCTAAATATATGTCTGAGTTCAAGAAAATGGCGATCCAATTCGCCATGGAGAGAGCTCTGAGTGCCGAGAGCCACACCAAGGGTGTCGGTTTGAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATTCTTCAGCTCAACCACACTTTAGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACGGCCCTAACCAACCTTGAAACTTGCCGCGCCGGGTTTGCTGAGCTTAATGTTTCAGACTATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCAGGGAAAGAGACATATAAAGAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGACAGGAGGCTACTGCAGTCATCTACAACAAATGCGAACCTTGTCGTGGCTCAGGATGGATCCGGTGACCATAAGACGGTTGGGGCGGCTCTAGAGGCAGCTGCAAAAGGGAAGACAGATGGGAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAGAACCTTGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTAGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCAGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCGTAACACAGCTGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCTGGTGCTGATCTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGACACCTCTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACAGAGGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACTGATCCAAACCAAAATACAGGCATTTCAATCCACAACTCACGGGTCATGGCCACAGATGACCTTAAACCAGTGGAGAGCACAGTGAGAACCTACTTGGGAAGGCCGTGGAAGGAGTTCTCTCGAACAGTTTTCATGCAAACTACCTCGATAGTTTGGTTGATCCAGCAGTATAAGAACAATGGTCCTCGTTCGTCAACCAGCCAGAGAGTGAAGTGGAAGGGTTACCATGCTATAAGCAATTCAACAGAAGTATCGAAGTTCACTGTCCAAAATTTCATCGCAGGACAGTCATGGTTGCCAGGCACAGAGCCAGCCTCACGCGTTTCCCTCCAATCTAAGAACAGAGACACCTGGGCCTTCGCCCAGAACGCAAAGCTCAACTCCAGTCTTGAAATGGGCAAGCCAGGAAACCTCTTAACAAAGACCCCGCCTAATAGGAAGAAACAACCTTCAACCACTTTGGCGCCCCCACTCGGGTAA

Protein sequence

MTALSMERSLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAPKYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNSIRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVRTYLGRPWKEFSRTVFMQTTSIVWLIQQYKNNGPRSSTSQRVKWKGYHAISNSTEVSKFTVQNFIAGQSWLPGTEPASRVSLQSKNRDTWAFAQNAKLNSSLEMGKPGNLLTKTPPNRKKQPSTTLAPPLG
Homology
BLAST of Sgr030035 vs. NCBI nr
Match: XP_022141175.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-230
Identity = 417/515 (80.97%), Postives = 438/515 (85.05%), Query Frame = 0

Query: 3   ALSMERSLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAPKYM 62
           AL ME+S GVLFFL FF LSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGS AAPK M
Sbjct: 37  ALLMEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDM 96

Query: 63  SEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSST 122
           SEFKKM  +FAMERAL AESH KGVG KCRNEKEKAAWADCLELYQNTILQLNHTLDSS+
Sbjct: 97  SEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSS 156

Query: 123 KCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWP 182
           KCTEFDIQTWLSTALTNLETC  GFAE NVSD+VLPLIMSNNVTKLISNSLAINNASAWP
Sbjct: 157 KCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWP 216

Query: 183 GKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFV 242
           GKETYKEGFPSWL GGDRRLLQSS+   +LVVAQDGSGDH+TVGAALEAAAK KT GRFV
Sbjct: 217 GKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFV 276

Query: 243 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 302
           IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA
Sbjct: 277 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 336

Query: 303 RGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------------ 362
           RGITFRNTAGPENHQAVALRSGAD SVFYRCGFEGYQDT + +H                
Sbjct: 337 RGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDT-LYVHSQRQFYKECYIYGTVD 396

Query: 363 ---------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVES 422
                    +++ +  A+RPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VES
Sbjct: 397 FIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVES 456

Query: 423 TVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPRSSTSQ 479
           TV+TYLGRPWKE+SRTVFMQT   S+V    WL              +YKN GP SSTSQ
Sbjct: 457 TVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQ 516

BLAST of Sgr030035 vs. NCBI nr
Match: XP_038882414.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 770.8 bits (1989), Expect = 7.8e-219
Identity = 400/512 (78.12%), Postives = 427/512 (83.40%), Query Frame = 0

Query: 6   MERSLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAPKYMSEF 65
           MERSLGV FFL FFLLSS AFSKHASNGIDWWC KTTYPETCKYFFN GSKAAPK M+EF
Sbjct: 1   MERSLGVFFFLHFFLLSSAAFSKHASNGIDWWCRKTTYPETCKYFFNQGSKAAPKDMTEF 60

Query: 66  KKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCT 125
           KKM IQFAMERAL AESHTKGVG KCRN KEKAAWADCL+LYQNTI QLN TLDS+TKCT
Sbjct: 61  KKMVIQFAMERALGAESHTKGVGSKCRNGKEKAAWADCLKLYQNTIFQLNQTLDSATKCT 120

Query: 126 EFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKE 185
           EFDIQTWLSTALTNLETCR GFAELNVSDY+L LIMSNNVTKLISNSLAINNASA  GKE
Sbjct: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYILQLIMSNNVTKLISNSLAINNASAGLGKE 180

Query: 186 TYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHV 245
           TYK+GFPSWLSGGDRRLLQSS+T  +LVVAQDGSG+H TVGAALE AAK KT+GRFVI V
Sbjct: 181 TYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNHTTVGAALEEAAKRKTNGRFVIQV 240

Query: 246 KRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGI 305
           KRGVY+ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTGEGFIARGI
Sbjct: 241 KRGVYQENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGI 300

Query: 306 TFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS--------------- 365
           TFRNTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H                   
Sbjct: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYIYGTVDFIF 360

Query: 366 ------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVR 425
                 +++ +  A+RPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTV+
Sbjct: 361 GNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVK 420

Query: 426 TYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPRSSTSQRVK 479
           TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GPRS TSQRV 
Sbjct: 421 TYLGRPWKEYSRTVFMKTYLDSLVDPTGWLEWDGDFALDTLYYGEYNNIGPRSPTSQRVN 480

BLAST of Sgr030035 vs. NCBI nr
Match: KAA0067674.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 748.4 bits (1931), Expect = 4.2e-212
Identity = 394/520 (75.77%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSL-GVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSME SL GV  FL FFLLSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTKCTEFDIQTWLSTALTNLETCR GFAELNV DY+ PLIMS+NVTKLISNSLAINNAS
Sbjct: 121 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GK TYK+GFPSWLSGGDRRLLQS +T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 181 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 360

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480

BLAST of Sgr030035 vs. NCBI nr
Match: XP_004148139.2 (pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [Cucumis sativus])

HSP 1 Score: 745.3 bits (1923), Expect = 3.5e-211
Identity = 393/520 (75.58%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSLGVLFFLQFFLL-SSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSM  S GV  FL FFLL SS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 46  MAGLSMGTSFGVFIFLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTK TEFDIQTWLSTALTNLETCR GFAELNVSDY+LPLIMS+NVT+LISNSLAINNAS
Sbjct: 166 SSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNAS 225

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GKETYK+GFPSWLSGGDRRLLQSS+T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 226 AGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 346 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 405

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 406 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 465

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 466 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPG 525

BLAST of Sgr030035 vs. NCBI nr
Match: XP_008439095.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 744.2 bits (1920), Expect = 7.9e-211
Identity = 393/520 (75.58%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSL-GVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSME SL GV  FL FFLLSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTK TEFDIQTWLSTALTNLETCR GFAELNV DY+ PLIMS+NVTKLISNSLAINNAS
Sbjct: 121 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GK TYK+GFPSWLSGGDRRLLQS++T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 181 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 360

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480

BLAST of Sgr030035 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 262/502 (52.19%), Postives = 345/502 (68.73%), Query Frame = 0

Query: 16  LQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP-KYMSEFKKMAIQFAM 75
           L  F LS  +F  ++   +  WC KT  P+ C+YF    +     K  ++F K+++Q A+
Sbjct: 11  LVLFSLSHTSFG-YSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLAL 70

Query: 76  ERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCTEFDIQTWLS 135
           ERA +A+S T  +G KCRNE+EKAAW DC ELY+ T+L+LN T +SS  CT+ D QTWLS
Sbjct: 71  ERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLS 130

Query: 136 TALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSW 195
           TALTNLETCRA   +L V +YVLPL +SNNVTKLISN+L++N       + +YK+GFP+W
Sbjct: 131 TALTNLETCRASLEDLGVPEYVLPL-LSNNVTKLISNTLSLNKVPY--NEPSYKDGFPTW 190

Query: 196 LSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHVKRGVYEENL 255
           +  GDR+LLQ +T  AN+VVAQDGSG+ KT+  A+ AA++     R+VI++K G Y EN+
Sbjct: 191 VKPGDRKLLQ-TTPRANIVVAQDGSGNVKTIQEAVAAASRA-GGSRYVIYIKAGTYNENI 250

Query: 256 EIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPE 315
           E+  K+KNIM VGDG+  TIITGS+SV GG+TTF SATVAV G+ FIAR IT RNTAGP 
Sbjct: 251 EV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPN 310

Query: 316 NHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS---------------------IRS 375
           NHQAVALRSG+DLSVFYRC FEGYQDT + +H                         +++
Sbjct: 311 NHQAVALRSGSDLSVFYRCSFEGYQDT-LYVHSQRQFYRECDIYGTVDFIFGNAAVVLQN 370

Query: 376 VIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVRTYLGRPWKE 435
               A++P N + N +TAQGRTDPNQ+TGI IHN RV A  DLKPV+S+V+T+LGRPWK+
Sbjct: 371 CNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQ 430

Query: 436 FSRTVFMQT--TSIV----WL------------IQQYKNNGPRSSTSQRVKWKGYHAISN 478
           +SRTV+++T   S++    W+              +Y N GP SST+ RVKW+GYH +++
Sbjct: 431 YSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTS 490

BLAST of Sgr030035 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 466.1 bits (1198), Expect = 5.4e-130
Identity = 253/507 (49.90%), Postives = 341/507 (67.26%), Query Frame = 0

Query: 12  VLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP-KYMSEFKKMAI 71
           ++ F+   +L +   S +    +  WCS+T  P+ C+YF  H S   P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 72  QFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCTEFDIQ 131
           +  ++RA+ A++H   +G KCR+ +EKAAW DC++LY  T+ ++N T+D + KC++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 132 TWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEG 191
           TWLSTALTNL+TCRAGF EL V+D VLPL MSNNV+ L+ N+LAIN    +      K+G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 192 FPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHVKRGVY 251
           FPSW+  GDR+LLQSST   N VVA+DGSG+ KT+  A++AA+     GRFVI+VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVY 247

Query: 252 EENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNT 311
            ENLEI  + KN+ML GDG+  TIITGS+SV GG+TTF+SATVA  G+GFIARGITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 312 AGPENHQAVALRSGADLSVFYRCGFEGYQDT-----SMSIHKDNSIRSVI---------- 371
           AG  N QAVALRSG+DLSVFY+C FE YQDT     +   ++D  +   +          
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 372 -----FTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVRTYLGRP 431
                  A+RP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + +TYLGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 432 WKEFSRTVFMQTT--SIV----WL------------IQQYKNNGPRSSTSQRVKWKGYHA 480
           W+++SRTVFM+T+  S++    WL              +++N GP +STS RV W G+  
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

BLAST of Sgr030035 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 249/529 (47.07%), Postives = 328/529 (62.00%), Query Frame = 0

Query: 6   MERSLGVLF---FLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSKAAPKY 65
           M + L +LF    L   L   P  +    N  D WC KT YP+ CK YF NH     P  
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 66  MSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTL--- 125
           +SEF+ M ++ AM+RA+SA +     G  C + K++A  ADC++LY +TI+QLN TL   
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 126 ----DSSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLA 185
                ++  CT+FD QTWLSTALTN ETCR G ++LNV+D++ P++ +  ++ LISN LA
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 183

Query: 186 INNASAWPGKE----TYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALE 245
           +N A    G +      ++GFP+WLS  D+RLL++    ANLVVA+DGSG   TV AA++
Sbjct: 184 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAID 243

Query: 246 AAAKGK-TDGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFH 305
            A + K T GRFVI+VKRG+Y+EN+ +     +IMLVGDGMR TIITG RSV+GG TT++
Sbjct: 244 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 303

Query: 306 SATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDN 365
           SAT  + G  FIA+GITFRNTAGP   QAVALRS +DLS+FY+C  EGYQDT M +H   
Sbjct: 304 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLM-VHSQR 363

Query: 366 S---------------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNS 425
                                  ++ +   +RP+ GQ N +TAQGR DP QNTGISIHNS
Sbjct: 364 QFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNS 423

Query: 426 RVMATDDLKPVESTVRTYLGRPWKEFSRTVFMQT------TSIVW--------------L 478
           R++   DLKPV  TV+TY+GRPW +FSRTV +QT      + + W               
Sbjct: 424 RILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLF 483

BLAST of Sgr030035 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 8.4e-123
Identity = 244/521 (46.83%), Postives = 319/521 (61.23%), Query Frame = 0

Query: 9   SLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSKAAPKYMSEFKK 68
           SL +L  L   L      +   S  ID WC KT YP  CK YF  H     P  +SEF+ 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRV 72

Query: 69  MAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD-----SST 128
           + ++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN TL      +  
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 129 KCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWP 188
           +CT+FD QTWLSTALTN ETCR G ++LNVSD+  P++ +  ++ LISN LA+N A    
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 189 GKETY----KEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGK-T 248
           GK        +GFP+W+S  +RRLLQ  +  ANLVVA+DGSG  KTV AA++ A + K T
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 252

Query: 249 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 308
            GRFVI+VKRG+Y+ENL +     NIMLVGDGMR+TIITG RSV+GG TT+ SAT  + G
Sbjct: 253 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

Query: 309 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 368
             FIA+GI F+NTAGP   QAVALRS +DLS+FYRC  EGYQDT M +H           
Sbjct: 313 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLM-VHSQRQFYRECYI 372

Query: 369 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 428
                          ++ I   + P+ GQ N +TAQGRTD  QNTGISIHNS ++   DL
Sbjct: 373 YGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDL 432

Query: 429 KPVESTVRTYLGRPWKEFSRTVFMQT------TSIVW--------------LIQQYKNNG 478
           KPV  +V+TY+GRPW  +SRTV ++T      + + W                 +YKN G
Sbjct: 433 KPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIG 492

BLAST of Sgr030035 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 8.1e-118
Identity = 233/515 (45.24%), Postives = 319/515 (61.94%), Query Frame = 0

Query: 23  SPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSK-----AAPKYMSEFKKMAIQFAMERA 82
           SP  +   S  + WWC+KT + ETC Y+F   S+       P++ SEF +M ++ A+++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 83  LSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTL-------DSSTKCTEFDIQ 142
           +   S T   G  C N + KAAW+DC+ L+QNT+ QLN TL        S  KCT+FD Q
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQ 192

Query: 143 TWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKET---- 202
           TWLSTA TN+ETCR+G  +LNVSD+V+P+I + N++ LI N LA+N         T    
Sbjct: 193 TWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTAN 252

Query: 203 YKEGFPSWLSGGDRRLLQSST---TNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVI 262
           +KE FPSW+S  +RRLL S++   ++ +LVVAQD SG  +++ AA+  AA+ +   RFVI
Sbjct: 253 HKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVI 312

Query: 263 HVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIAR 322
           +VK+GVY EN+++GN   NIMLVGDG R TIIT  RSV+ G TT++SAT    G+ F+A+
Sbjct: 313 YVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAK 372

Query: 323 GITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------------- 382
            +TF NTAGP   QAVA+RS +DLSVFYR G  G+QDT + IH                 
Sbjct: 373 DMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDT-LYIHSQRQFFRECYISGTIDF 432

Query: 383 --------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 442
                    ++ +   +RP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV   
Sbjct: 433 IFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRA 492

Query: 443 VRTYLGRPWKEFSRTVFMQT------TSIVW--------------LIQQYKNNGPRSSTS 478
            +TYLGRPW+ +SR   M+T      + + W                 +YKN GP SST 
Sbjct: 493 YKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTR 552

BLAST of Sgr030035 vs. ExPASy TrEMBL
Match: A0A6J1CH86 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.6e-231
Identity = 417/515 (80.97%), Postives = 438/515 (85.05%), Query Frame = 0

Query: 3   ALSMERSLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAPKYM 62
           AL ME+S GVLFFL FF LSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGS AAPK M
Sbjct: 37  ALLMEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDM 96

Query: 63  SEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSST 122
           SEFKKM  +FAMERAL AESH KGVG KCRNEKEKAAWADCLELYQNTILQLNHTLDSS+
Sbjct: 97  SEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSS 156

Query: 123 KCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWP 182
           KCTEFDIQTWLSTALTNLETC  GFAE NVSD+VLPLIMSNNVTKLISNSLAINNASAWP
Sbjct: 157 KCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWP 216

Query: 183 GKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFV 242
           GKETYKEGFPSWL GGDRRLLQSS+   +LVVAQDGSGDH+TVGAALEAAAK KT GRFV
Sbjct: 217 GKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFV 276

Query: 243 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 302
           IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA
Sbjct: 277 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 336

Query: 303 RGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------------ 362
           RGITFRNTAGPENHQAVALRSGAD SVFYRCGFEGYQDT + +H                
Sbjct: 337 RGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDT-LYVHSQRQFYKECYIYGTVD 396

Query: 363 ---------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVES 422
                    +++ +  A+RPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VES
Sbjct: 397 FIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVES 456

Query: 423 TVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPRSSTSQ 479
           TV+TYLGRPWKE+SRTVFMQT   S+V    WL              +YKN GP SSTSQ
Sbjct: 457 TVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQ 516

BLAST of Sgr030035 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 2.0e-212
Identity = 394/520 (75.77%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSL-GVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSME SL GV  FL FFLLSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTKCTEFDIQTWLSTALTNLETCR GFAELNV DY+ PLIMS+NVTKLISNSLAINNAS
Sbjct: 121 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GK TYK+GFPSWLSGGDRRLLQS +T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 181 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 360

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480

BLAST of Sgr030035 vs. ExPASy TrEMBL
Match: A0A0A0L9G4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.7e-211
Identity = 393/520 (75.58%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSLGVLFFLQFFLL-SSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSM  S GV  FL FFLL SS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 46  MAGLSMGTSFGVFIFLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTK TEFDIQTWLSTALTNLETCR GFAELNVSDY+LPLIMS+NVT+LISNSLAINNAS
Sbjct: 166 SSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNAS 225

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GKETYK+GFPSWLSGGDRRLLQSS+T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 226 AGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 346 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 405

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 406 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 465

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 466 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPG 525

BLAST of Sgr030035 vs. ExPASy TrEMBL
Match: A0A1S3AYJ9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 3.8e-211
Identity = 393/520 (75.58%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 1   MTALSMERSL-GVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP 60
           M  LSME SL GV  FL FFLLSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+K+ P
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 61  KYMSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD 120
           K M++FKKM IQFAMERALSAESHTKGVG KCRN KEKAAWADCL+LYQNTILQLN TLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 121 SSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNAS 180
           SSTK TEFDIQTWLSTALTNLETCR GFAELNV DY+ PLIMS+NVTKLISNSLAINNAS
Sbjct: 121 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 181 A--WPGKETYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKT 240
           A    GK TYK+GFPSWLSGGDRRLLQS++T  +LVVAQDGSG++ TVGAALE AAK KT
Sbjct: 181 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 241 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 300
            GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 301 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 360
           EGFIARGITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDT + +H           
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDT-LYVHSQRQFYKECYI 360

Query: 361 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
                         +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420

Query: 421 KPVESTVRTYLGRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPR 479
           KPVESTV+TYLGRPWKE+SRTVFM+T   S+V    WL              +Y N GP 
Sbjct: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480

BLAST of Sgr030035 vs. ExPASy TrEMBL
Match: A0A6J1FT96 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447927 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 6.3e-206
Identity = 373/509 (73.28%), Postives = 413/509 (81.14%), Query Frame = 0

Query: 9   SLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAPKYMSEFKKM 68
           +LGVLF + FFLLSS AFSKH SNGI WWC+KTTYPETCKYF+ H +   PK MSEFKKM
Sbjct: 5   TLGVLFLIHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSEFKKM 64

Query: 69  AIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCTEFD 128
           AI+FAM+ ALS E HTK VG +CRN KE+ AWADCL+LYQNTI QL  TLDSS K TEFD
Sbjct: 65  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKSTEFD 124

Query: 129 IQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKETYK 188
           IQTWLSTALTN+ETCR GFAELNV D VLPLI SNNVT+LISNSLA+NNAS W GKETYK
Sbjct: 125 IQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGKETYK 184

Query: 189 EGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHVKRG 248
           +GFPSWLSGGDR+LLQSS+   +LVVAQDGSGDHKTV AALE AAK +TDGRFVI VKRG
Sbjct: 185 QGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRRTDGRFVIQVKRG 244

Query: 249 VYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFR 308
           VYEENLEIGNKMKNIMLVGDGMRFT ITGSRSV GGSTTF+SATVAVTGEGFIA+GITFR
Sbjct: 245 VYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQGITFR 304

Query: 309 NTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------------------ 368
           NTAGPENHQAVALRSGAD SVFYRC FEGYQDT + +H                      
Sbjct: 305 NTAGPENHQAVALRSGADRSVFYRCAFEGYQDT-LYVHSQRQFYKECYIYGTVDFIFGNA 364

Query: 369 ---IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVRTYL 428
              +++ +  A++PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTV+T+L
Sbjct: 365 AVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTFL 424

Query: 429 GRPWKEFSRTVFMQT--TSIV----WL------------IQQYKNNGPRSSTSQRVKWKG 479
           GRPWKE+SRTVF++T   ++V    WL              +YKN GPRSST QRV+WKG
Sbjct: 425 GRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKG 484

BLAST of Sgr030035 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 466.1 bits (1198), Expect = 3.8e-131
Identity = 253/507 (49.90%), Postives = 341/507 (67.26%), Query Frame = 0

Query: 12  VLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSKAAP-KYMSEFKKMAI 71
           ++ F+   +L +   S +    +  WCS+T  P+ C+YF  H S   P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 72  QFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLDSSTKCTEFDIQ 131
           +  ++RA+ A++H   +G KCR+ +EKAAW DC++LY  T+ ++N T+D + KC++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 132 TWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEG 191
           TWLSTALTNL+TCRAGF EL V+D VLPL MSNNV+ L+ N+LAIN    +      K+G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 192 FPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVIHVKRGVY 251
           FPSW+  GDR+LLQSST   N VVA+DGSG+ KT+  A++AA+     GRFVI+VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVY 247

Query: 252 EENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNT 311
            ENLEI  + KN+ML GDG+  TIITGS+SV GG+TTF+SATVA  G+GFIARGITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 312 AGPENHQAVALRSGADLSVFYRCGFEGYQDT-----SMSIHKDNSIRSVI---------- 371
           AG  N QAVALRSG+DLSVFY+C FE YQDT     +   ++D  +   +          
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 372 -----FTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVRTYLGRP 431
                  A+RP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + +TYLGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 432 WKEFSRTVFMQTT--SIV----WL------------IQQYKNNGPRSSTSQRVKWKGYHA 480
           W+++SRTVFM+T+  S++    WL              +++N GP +STS RV W G+  
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

BLAST of Sgr030035 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-126
Identity = 249/529 (47.07%), Postives = 328/529 (62.00%), Query Frame = 0

Query: 6   MERSLGVLF---FLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSKAAPKY 65
           M + L +LF    L   L   P  +    N  D WC KT YP+ CK YF NH     P  
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 66  MSEFKKMAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTL--- 125
           +SEF+ M ++ AM+RA+SA +     G  C + K++A  ADC++LY +TI+QLN TL   
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 126 ----DSSTKCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLA 185
                ++  CT+FD QTWLSTALTN ETCR G ++LNV+D++ P++ +  ++ LISN LA
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 183

Query: 186 INNASAWPGKE----TYKEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALE 245
           +N A    G +      ++GFP+WLS  D+RLL++    ANLVVA+DGSG   TV AA++
Sbjct: 184 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAID 243

Query: 246 AAAKGK-TDGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFH 305
            A + K T GRFVI+VKRG+Y+EN+ +     +IMLVGDGMR TIITG RSV+GG TT++
Sbjct: 244 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 303

Query: 306 SATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDN 365
           SAT  + G  FIA+GITFRNTAGP   QAVALRS +DLS+FY+C  EGYQDT M +H   
Sbjct: 304 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLM-VHSQR 363

Query: 366 S---------------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNS 425
                                  ++ +   +RP+ GQ N +TAQGR DP QNTGISIHNS
Sbjct: 364 QFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNS 423

Query: 426 RVMATDDLKPVESTVRTYLGRPWKEFSRTVFMQT------TSIVW--------------L 478
           R++   DLKPV  TV+TY+GRPW +FSRTV +QT      + + W               
Sbjct: 424 RILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLF 483

BLAST of Sgr030035 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 442.2 bits (1136), Expect = 5.9e-124
Identity = 244/521 (46.83%), Postives = 319/521 (61.23%), Query Frame = 0

Query: 9   SLGVLFFLQFFLLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSKAAPKYMSEFKK 68
           SL +L  L   L      +   S  ID WC KT YP  CK YF  H     P  +SEF+ 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRV 72

Query: 69  MAIQFAMERALSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTLD-----SST 128
           + ++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN TL      +  
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 129 KCTEFDIQTWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWP 188
           +CT+FD QTWLSTALTN ETCR G ++LNVSD+  P++ +  ++ LISN LA+N A    
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 189 GKETY----KEGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAAKGK-T 248
           GK        +GFP+W+S  +RRLLQ  +  ANLVVA+DGSG  KTV AA++ A + K T
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 252

Query: 249 DGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTG 308
            GRFVI+VKRG+Y+ENL +     NIMLVGDGMR+TIITG RSV+GG TT+ SAT  + G
Sbjct: 253 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

Query: 309 EGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------- 368
             FIA+GI F+NTAGP   QAVALRS +DLS+FYRC  EGYQDT M +H           
Sbjct: 313 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLM-VHSQRQFYRECYI 372

Query: 369 --------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 428
                          ++ I   + P+ GQ N +TAQGRTD  QNTGISIHNS ++   DL
Sbjct: 373 YGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDL 432

Query: 429 KPVESTVRTYLGRPWKEFSRTVFMQT------TSIVW--------------LIQQYKNNG 478
           KPV  +V+TY+GRPW  +SRTV ++T      + + W                 +YKN G
Sbjct: 433 KPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIG 492

BLAST of Sgr030035 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 425.6 bits (1093), Expect = 5.8e-119
Identity = 233/515 (45.24%), Postives = 319/515 (61.94%), Query Frame = 0

Query: 23  SPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSK-----AAPKYMSEFKKMAIQFAMERA 82
           SP  +   S  + WWC+KT + ETC Y+F   S+       P++ SEF +M ++ A+++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 83  LSAESHTKGVGLKCRNEKEKAAWADCLELYQNTILQLNHTL-------DSSTKCTEFDIQ 142
           +   S T   G  C N + KAAW+DC+ L+QNT+ QLN TL        S  KCT+FD Q
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQ 192

Query: 143 TWLSTALTNLETCRAGFAELNVSDYVLPLIMSNNVTKLISNSLAINNASAWPGKET---- 202
           TWLSTA TN+ETCR+G  +LNVSD+V+P+I + N++ LI N LA+N         T    
Sbjct: 193 TWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTAN 252

Query: 203 YKEGFPSWLSGGDRRLLQSST---TNANLVVAQDGSGDHKTVGAALEAAAKGKTDGRFVI 262
           +KE FPSW+S  +RRLL S++   ++ +LVVAQD SG  +++ AA+  AA+ +   RFVI
Sbjct: 253 HKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVI 312

Query: 263 HVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIAR 322
           +VK+GVY EN+++GN   NIMLVGDG R TIIT  RSV+ G TT++SAT    G+ F+A+
Sbjct: 313 YVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAK 372

Query: 323 GITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS------------- 382
            +TF NTAGP   QAVA+RS +DLSVFYR G  G+QDT + IH                 
Sbjct: 373 DMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDT-LYIHSQRQFFRECYISGTIDF 432

Query: 383 --------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 442
                    ++ +   +RP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV   
Sbjct: 433 IFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRA 492

Query: 443 VRTYLGRPWKEFSRTVFMQT------TSIVW--------------LIQQYKNNGPRSSTS 478
            +TYLGRPW+ +SR   M+T      + + W                 +YKN GP SST 
Sbjct: 493 YKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTR 552

BLAST of Sgr030035 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-85
Identity = 209/523 (39.96%), Postives = 276/523 (52.77%), Query Frame = 0

Query: 38  CSKTTYPETCKYFFNHGSKAAPKYMSEFKKMAIQFAMERALSAESH---------TKGVG 97
           CS T YPE C   F+  +    K ++  +K  I+ ++     A  H          K  G
Sbjct: 66  CSSTLYPELC---FSAVAATGGKELTS-QKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 125

Query: 98  LKCRNEKEKAAWADCLELYQNTILQLNHTLD--------SSTKCTEFDIQTWLSTALTNL 157
           L     +E  A  DCLE    T+ +L+  ++         S +    D++T +S+A+TN 
Sbjct: 126 L---TPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQ 185

Query: 158 ETCRAGFAELNVSDYVLPLIMSN--NVTKLISNSLAI---------------NNASAWPG 217
            TC  GF+  +    V   ++    +V  + SN+LA+               + +S +  
Sbjct: 186 GTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTN 245

Query: 218 KETYK----------EGFPSWLSGGDRRLLQSSTTNANLVVAQDGSGDHKTVGAALEAAA 277
               K          +G+P WLS GDRRLLQ ST  A+  VA DGSGD  TV AA+ AAA
Sbjct: 246 NNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAV-AAA 305

Query: 278 KGKTDGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATV 337
             K++ RFVIH+K GVY EN+E+  K  NIM +GDG   TIITGSR+V  GSTTFHSATV
Sbjct: 306 PEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATV 365

Query: 338 AVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTSMSIHKDNS--- 397
           A  GE F+AR ITF+NTAGP  HQAVALR G+D S FY+C    YQDT + +H +     
Sbjct: 366 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDT-LYVHSNRQFFV 425

Query: 398 ------------------IRSVIFTAQRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMA 457
                             ++     A+RP +GQKN VTAQGR+DPNQNTGI I N R+  
Sbjct: 426 KCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGG 485

Query: 458 TDDLKPVESTVRTYLGRPWKEFSRTVFMQT--TSIV-------W---------LIQQYKN 478
           T DL  V+ T  TYLGRPWKE+SRTV MQ+  + ++       W           ++Y N
Sbjct: 486 TSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLN 545

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141175.11.2e-23080.97pectinesterase-like [Momordica charantia][more]
XP_038882414.17.8e-21978.13pectinesterase-like [Benincasa hispida][more]
KAA0067674.14.2e-21275.77pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [... [more]
XP_004148139.23.5e-21175.58pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [... [more]
XP_008439095.27.9e-21175.58PREDICTED: pectinesterase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
O048871.4e-13352.19Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221495.4e-13049.90Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN51.8e-12547.07Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN48.4e-12346.83Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY38.1e-11845.24Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1CH865.6e-23180.97Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1[more]
A0A5D3DJL72.0e-21275.77Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440... [more]
A0A0A0L9G41.7e-21175.58Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1[more]
A0A1S3AYJ93.8e-21175.58Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1[more]
A0A6J1FT966.3e-20673.28Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447927 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G45220.13.8e-13149.90Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.11.3e-12647.07Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.15.9e-12446.83Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.15.8e-11945.24Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.11.4e-8539.96pectin methylesterase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 29..175
e-value: 5.8E-32
score: 122.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..177
e-value: 5.3E-26
score: 89.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 34..175
e-value: 4.2E-26
score: 92.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 211..342
e-value: 2.1E-53
score: 181.0
coord: 356..423
e-value: 2.3E-26
score: 92.2
coord: 431..470
e-value: 6.2E-10
score: 38.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..178
e-value: 6.6E-29
score: 102.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 32..177
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 205..484
e-value: 7.7E-78
score: 264.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 504..534
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 12..342
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 355..478
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 355..478
coord: 12..342
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 36..178
e-value: 1.06638E-34
score: 126.014
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 206..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030035.1Sgr030035.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity