Sgr029987 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr029987
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionFe2OG dioxygenase domain-containing protein
Locationtig00153554: 1810771 .. 1812471 (+)
RNA-Seq ExpressionSgr029987
SyntenySgr029987
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGGAAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACAACGCCCAAGTTTATCCCCGGAGATCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCGCCATTGAAGAGCTCAGAAAAGCTTGCATTCATCTAGGCTTCTTTCAGGTAAGTTTTAAGCTACCTACCTACCTCTTTTAGACTTCATTCTGATCCTGGAAAGATTCATTTGACCTCTTAACTTCAACTTTTTTCTTCAGATAATCAACCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTCGGCCAAGCCTACGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTACAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTTCTGGCCCACCAATCCAAGTAGCTATAGGTAACTTGATATTTTAGTTCTCCATCTTCACTTCTAAGCTGATTTTCCATTCTTTTTTTCTTTTCCTGAATACCATGCAAAATTTACTACACAAGAGGGCTGTACCATGAAATTCAACAGAAAGAGACAATAATGAAACAACTGCACCTCCAAAAGAGAGCACCGCTATACATATGCTGCTGCCTTTGAACCAGAATTCCAGCTATAACAATGATATTTTTATATATGCTAATGTTTTGTATGGTCTAGATCAGAGTTTGAATTTTTCTACGCATACTCAGCGTACTATCAAGGCATTCTTCTTCAATGAACCAAAAATATAGGAGTTGAAACGCACTCATCCAATAAATGACACATGACAACTTATAAAAGCACTTCTTACTGAGAGTTGATGTAGATATCGACAAAAATATCGACGTCGATAGAAATGCAGTCTTTTGCTATGGGAAGACCAACTTGTCAATAGAAATTTTTTATTTGAAAGAAATTAAGAGTCTGGGAGCATATTTCTTAGATACTATGTATATATGTACTCTCCTTTCAGGGAAAAGATGGGAAGATACACCAGGGAGGTGAGAAAAGTGAGCTTAGAAGTAATGGAAGCCATAACTGAGAGCTTGGGTTTAGGGCCAACATACCTTAGCAGCAAAATGGCTGAAGGAGTGCAGGTAGTGGCAGTAAACTGCTACCCCCCATGTCCACAGCCTGAGGTCGCACTTGGGTTGCCGCCACATACAGACTACAGTTGCATAACCACAGTTCTTCAAAGCAGCCAGGGCCTTGAAATCATGGACATGGCTGATGGGACATGGAAGCTGGTTCCAAAAGTTGATGGCGCGCTGCAAGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGGCTTTACAAGGGCGTTATCCACAGAGCCACGGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGAAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTATGGATGAACAAAACCCAAAGAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGTAATGATCTTGCAGAAGGGAAGAGCTTCATCAACACACTCAAGATCCAGGAATAA

mRNA sequence

ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGGAAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACAACGCCCAAGTTTATCCCCGGAGATCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCGCCATTGAAGAGCTCAGAAAAGCTTGCATTCATCTAGGCTTCTTTCAGATAATCAACCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTCGGCCAAGCCTACGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTACAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTTCTGGCCCACCAATCCAAGTAGCTATAGGGAAAAGATGGGAAGATACACCAGGGAGGTGAGAAAAGTGAGCTTAGAAGTAATGGAAGCCATAACTGAGAGCTTGGGTTTAGGGCCAACATACCTTAGCAGCAAAATGGCTGAAGGAGTGCAGGTAGTGGCAGTAAACTGCTACCCCCCATGTCCACAGCCTGAGGTCGCACTTGGGTTGCCGCCACATACAGACTACAGTTGCATAACCACAGTTCTTCAAAGCAGCCAGGGCCTTGAAATCATGGACATGGCTGATGGGACATGGAAGCTGGTTCCAAAAGTTGATGGCGCGCTGCAAGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGGCTTTACAAGGGCGTTATCCACAGAGCCACGGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGAAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTATGGATGAACAAAACCCAAAGAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGTAATGATCTTGCAGAAGGGAAGAGCTTCATCAACACACTCAAGATCCAGGAATAA

Coding sequence (CDS)

ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGGAAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACAACGCCCAAGTTTATCCCCGGAGATCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTCAAAGGTCCCTCGCCATTGAAGAGCTCAGAAAAGCTTGCATTCATCTAGGCTTCTTTCAGATAATCAACCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTCGGCCAAGCCTACGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTACAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTTCTGGCCCACCAATCCAAGTAGCTATAGGGAAAAGATGGGAAGATACACCAGGGAGGTGAGAAAAGTGAGCTTAGAAGTAATGGAAGCCATAACTGAGAGCTTGGGTTTAGGGCCAACATACCTTAGCAGCAAAATGGCTGAAGGAGTGCAGGTAGTGGCAGTAAACTGCTACCCCCCATGTCCACAGCCTGAGGTCGCACTTGGGTTGCCGCCACATACAGACTACAGTTGCATAACCACAGTTCTTCAAAGCAGCCAGGGCCTTGAAATCATGGACATGGCTGATGGGACATGGAAGCTGGTTCCAAAAGTTGATGGCGCGCTGCAAGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGGCTTTACAAGGGCGTTATCCACAGAGCCACGGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGAAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTATGGATGAACAAAACCCAAAGAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGTAATGATCTTGCAGAAGGGAAGAGCTTCATCAACACACTCAAGATCCAGGAATAA

Protein sequence

MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSAQRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE
Homology
BLAST of Sgr029987 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 2.3e-182
Identity = 317/354 (89.55%), Postives = 337/354 (95.20%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GESSG+FPTGKTAQEKGLSYVPNAYVIPAP RPS+SPEIAIVPIIDMASLRS+DS 
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  QRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QRSLA EELRKACI LGFFQIINHGI++TVMEEAL QA EFF+LPLKEKMKYKSDDVC+A
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEV 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID WPTNP +YREKMGRYT+EVRKVSLE+
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 MEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAI ESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITTVLQSSQ
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLH 300
           GLE+MD ADGTWK+VPK+DGALQVHVGDHVEVLSNGLYKGV+HRATVNSE TRISITSLH
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

Query: 301 SLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           SL MDEKMKPAEEL++E NPKKYK+SSFNDFLKFLSSNDLAEGKSF+NTLK+Q+
Sbjct: 303 SLGMDEKMKPAEELVNEHNPKKYKDSSFNDFLKFLSSNDLAEGKSFLNTLKLQD 356

BLAST of Sgr029987 vs. NCBI nr
Match: XP_008453850.1 (PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa] >TYK16900.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 647.5 bits (1669), Expect = 6.6e-182
Identity = 318/354 (89.83%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GE SG+FPTGKTAQEKGLSYVPNAYVIPAP RPSLSPEIAIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QRSLA+EELRKACI LGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPTNP +YREKMG+YT+EVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V K+DGALQVHVGDHVEVLSNGLYKGV+HRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           SL MDEKMKPAEEL++EQNPKKYKESSFNDFLKFLSSNDLAEG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Sgr029987 vs. NCBI nr
Match: XP_022140813.1 (flavanone 3-dioxygenase 3 [Momordica charantia])

HSP 1 Score: 625.9 bits (1613), Expect = 2.1e-175
Identity = 319/381 (83.73%), Postives = 333/381 (87.40%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GESSGSFPTG++AQEKGLSYVPNAYVIPAP R S S E AI+PIIDMASLRS DSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLAIEELRKACIHLGFF---------------------------QIINHGISKTVMEE 120
           QRSLAIEELR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID 180
           AL QAYEFFDLPLKEKMKYKSDDV RAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 FWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPP 240
            WPT+P SYREKMG YTREVRKV LEVMEAITESLGL PTYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVL 300
           CPQPEVALGLPPHTDYSCITT+LQSS+GLEIMDMADGTWKLVP +DGALQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLK 355
           SNGLYKGV+HRATVNSESTRISITSLHSL MDEKMKPAEEL+D+QNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of Sgr029987 vs. NCBI nr
Match: XP_022922584.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata])

HSP 1 Score: 625.2 bits (1611), Expect = 3.5e-175
Identity = 316/376 (84.04%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGLKGESSGSF TG+TAQEKGL+YVPNAYVIP PQRPSLSPE AIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACIHLGFF----------------------QIINHGISKTVMEEALGQA 120
           QRSL IE LRKACI LGFF                      QIINHGISKTVMEEAL QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 YEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTN 180
            EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+D WPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPE 240
           P +YREKMG+YT+EVRKVSLE+ EAITESLGL PTYLSSKMAEGVQVVAVNCYPPCPQP 
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP VDG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSN 355
           KGV+HRA VNSE TRISI+SLHSL MDEKMKPAEEL+DEQNP KYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Sgr029987 vs. NCBI nr
Match: XP_038889497.1 (flavanone 3-dioxygenase 3 [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 6.0e-175
Identity = 309/350 (88.29%), Postives = 328/350 (93.71%), Query Frame = 0

Query: 5   GESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSAQRSL 64
           G  +GSFPTGKTAQEKG SYVPNAYVIPAP R SLSPEIA VPIIDMASLRSNDSAQRSL
Sbjct: 9   GTGTGSFPTGKTAQEKGFSYVPNAYVIPAPHRASLSPEIATVPIIDMASLRSNDSAQRSL 68

Query: 65  AIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYG 124
           AI+ELRKACI LGFFQIINHGIS+TVMEEAL QA EFF+LPLKEKMKYKSDDV +AVRYG
Sbjct: 69  AIQELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVRKAVRYG 128

Query: 125 TSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAI 184
           TSLKDGVDKIKFWRVFLKHYAHPLEDWID WPTNP +YREKMG+YT+ VRKVSLE+MEAI
Sbjct: 129 TSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKVVRKVSLEIMEAI 188

Query: 185 TESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEI 244
           TESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITTVLQS+QGLEI
Sbjct: 189 TESLSLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSNQGLEI 248

Query: 245 MDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEM 304
           MD ADGTWK+VPK+ GALQVH+GDHVEVLSNGLYKGV+HRATVNSE  RISITSLHSL M
Sbjct: 249 MDPADGTWKMVPKIAGALQVHLGDHVEVLSNGLYKGVVHRATVNSERARISITSLHSLGM 308

Query: 305 DEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           DEKMKPAEEL+DEQNP KYK+SSF+DFLKFLSSNDLAEGKSFINTLKI++
Sbjct: 309 DEKMKPAEELVDEQNPIKYKDSSFDDFLKFLSSNDLAEGKSFINTLKIED 358

BLAST of Sgr029987 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.6e-85
Identity = 154/322 (47.83%), Postives = 220/322 (68.32%), Query Frame = 0

Query: 31  IPAPQRPSLSPEIAIVPIIDMASLRSNDSAQRSLAIEELRKACIHLGFFQIINHGISKTV 90
           IP  Q PS       +P+I++  L  +D   RS  + ++ KAC  LG+FQ+I+HGIS++V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLED 150
           M+ A+  A EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLKHYAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNC 210
           W+ +WP  P  YRE M +Y  EVR V+L++MEAI E LG+G  Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHV 270
           YP   Q + ++GL  H+DY  +T +L S QGLE++D +  +WK+V ++  AL VHVGDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EVLSNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFND 330
           EVLSNG  K V+HRA +N +  RIS+ S+H   + EK+  A+EL+DE+NP+KYKE+SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

Query: 331 FLKFLSSNDLAEGKSFINTLKI 353
           FL+ L++N     ++F+ +L++
Sbjct: 308 FLEHLTANMDNRQRNFLESLRM 326

BLAST of Sgr029987 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-82
Identity = 157/319 (49.22%), Postives = 215/319 (67.40%), Query Frame = 0

Query: 37  PSLSPEIAIVPIIDMASLRSNDSAQRSLAIEELRKACIHLGFFQIINHGISKTVMEEALG 96
           P L P    +P+I++  L + DSA R+LA+ ++  AC   G F+++NHGIS++ M  AL 
Sbjct: 19  PPLPPPPRPLPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALE 78

Query: 97  QAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWP 156
            A EFF L  + K ++ SDD+ + +RY TS +DG   I   R FLKHYA+PL+DWI FWP
Sbjct: 79  AASEFFQLSTERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWP 138

Query: 157 TNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQ 216
           T P +YREKMG Y  E ++VS+++MEAI + LGLGP+YL  K+  GVQ VA+N YP    
Sbjct: 139 TQPPTYREKMGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSA 198

Query: 217 PEV-ALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSN 276
            +   +GL PH+DY  +T +LQSS GLE+M   D  W  VP + GAL VHVGDH+EVLSN
Sbjct: 199 KKADKIGLAPHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSN 258

Query: 277 GLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFL 336
           G  K ++HRA +N   +RISI S+H L MDE++  AEEL+DE +PK Y+ SSF DFL FL
Sbjct: 259 GQLKSLVHRAVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFL 318

Query: 337 SSNDLAEGKSFINTLKIQE 355
            +N +   K ++ +L+I +
Sbjct: 319 PAN-MNRYKRYVESLRIDK 332

BLAST of Sgr029987 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-67
Identity = 135/342 (39.47%), Postives = 203/342 (59.36%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPQRPSLSPEIAI-------VPIIDMASLRSNDSAQRSLAIEELRK 78
           + G++ +P  YV+PA  RP  +   A        +P++D++ LR    ++R   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDA 105

Query: 79  ACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L  A  FF+LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLG 198
           D +  WR FLK    PL   ++ WPT+P+  RE   RY    ++V +EVMEA  E+LG+G
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 PTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSS-QGLEIMDMADG 258
              +   +A G Q++ VNCYP CPQPE+ LG+PPH+DY  +T VLQ    GL++M    G
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHA--G 285

Query: 259 TWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKP 318
            W  V  + G+  V+VGDH+E+LSNG Y+ V+HR  VNS   R+S+ S HS+  +  + P
Sbjct: 286 EWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSP 345

Query: 319 AEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKI 353
           A EL+D+++P++Y ++    FL +L+S      KSF+++ ++
Sbjct: 346 APELIDDRHPRRYMDTDLATFLAYLAS-AAGNHKSFLHSRRL 382

BLAST of Sgr029987 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-60
Identity = 142/363 (39.12%), Postives = 211/363 (58.13%), Query Frame = 0

Query: 5   GESSGS--FPTG-KTAQEKGLSYVPNAYVIPAPQRPS--LSPEIAIVPIIDMASLRSNDS 64
           GE SGS  +  G +   + G++ +P  YV+P   RP+  ++P  A++P++D+A+LR+ D 
Sbjct: 13  GEGSGSSEWELGVRQLCDSGITTLPARYVLPPADRPARYVTPP-ALLPVVDLAALRARDP 72

Query: 65  AQRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCR 124
            Q    +  L  AC   GFFQ++NHG+    M  A  +   FFDLPL  + +Y S D+  
Sbjct: 73  CQ----LAALHAACRDYGFFQLLNHGVPPDAMLYAARRF--FFDLPLPARKRYMSADIRA 132

Query: 125 AVRYGTSLKDGVDKIKFWRVFLK---HYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKV 184
           AVRYGTS     D +  WR FLK        L D +  WP  P   R     Y    +++
Sbjct: 133 AVRYGTSFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRL 192

Query: 185 SLEVMEAITESLGL--GPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITT 244
             E+MEA  ++LG+      L  +   G Q++ VNC+P CP+PE+ LG+PPH+DY  +T 
Sbjct: 193 FRELMEAALDALGIVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTI 252

Query: 245 VLQSS-QGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTR 304
           +LQ   +GLE+     G W +V  + GA+ V+VGDH+E+LSNGLY+ V+HR  VN    R
Sbjct: 253 LLQDEVRGLEVSYGDGGGWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRAR 312

Query: 305 ISITSLHSLEMDEKMKPAEELMDEQ--NPKKYKESSFNDFLKFLSSNDLAEG--KSFINT 353
           +S+ SLHSL  +  + PA EL+DEQ   P++Y ++    FL +L+S   AEG  KSF+++
Sbjct: 313 VSVASLHSLAAERVIGPAAELVDEQRGRPRRYMDTDMAAFLAYLAS---AEGNHKSFLHS 365

BLAST of Sgr029987 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.5e-59
Identity = 119/331 (35.95%), Postives = 192/331 (58.01%), Query Frame = 0

Query: 25  VPNAYVIPAPQRPSLS--PEIAIVPIIDMASLRSNDSAQRSLAIEELRKACIHLGFFQII 84
           +P  YV P   RP LS   ++   P+ID++      S  RS  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +  A EFF + ++EKMK  SDD  +  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEG 204
            + +P+  +++ WP+NP S++E + +Y+REVR+V  ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGAL 264
            Q +AVN YPPCP+PE+  GLP HTD + +T +LQ +    +  + DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSL---HSLEMDEKMKPAEELMDEQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S        +    KP  E  D++ 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDLAEGKSFINTL 351
              YK+ ++ ++ K   S +L +     N L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339

BLAST of Sgr029987 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.2e-182
Identity = 318/354 (89.83%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GE SG+FPTGKTAQEKGLSYVPNAYVIPAP RPSLSPEIAIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QRSLA+EELRKACI LGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPTNP +YREKMG+YT+EVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V K+DGALQVHVGDHVEVLSNGLYKGV+HRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           SL MDEKMKPAEEL++EQNPKKYKESSFNDFLKFLSSNDLAEG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Sgr029987 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.2e-182
Identity = 318/354 (89.83%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GE SG+FPTGKTAQEKGLSYVPNAYVIPAP RPSLSPEIAIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QRSLA+EELRKACI LGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPTNP +YREKMG+YT+EVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V K+DGALQVHVGDHVEVLSNGLYKGV+HRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           SL MDEKMKPAEEL++EQNPKKYKESSFNDFLKFLSSNDLAEG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Sgr029987 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.0e-175
Identity = 319/381 (83.73%), Postives = 333/381 (87.40%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL GESSGSFPTG++AQEKGLSYVPNAYVIPAP R S S E AI+PIIDMASLRS DSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLAIEELRKACIHLGFF---------------------------QIINHGISKTVMEE 120
           QRSLAIEELR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID 180
           AL QAYEFFDLPLKEKMKYKSDDV RAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 FWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPP 240
            WPT+P SYREKMG YTREVRKV LEVMEAITESLGL PTYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVL 300
           CPQPEVALGLPPHTDYSCITT+LQSS+GLEIMDMADGTWKLVP +DGALQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLK 355
           SNGLYKGV+HRATVNSESTRISITSLHSL MDEKMKPAEEL+D+QNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of Sgr029987 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 316/376 (84.04%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGLKGESSGSF TG+TAQEKGL+YVPNAYVIP PQRPSLSPE AIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACIHLGFF----------------------QIINHGISKTVMEEALGQA 120
           QRSL IE LRKACI LGFF                      QIINHGISKTVMEEAL QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 YEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTN 180
            EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+D WPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPE 240
           P +YREKMG+YT+EVRKVSLE+ EAITESLGL PTYLSSKMAEGVQVVAVNCYPPCPQP 
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP VDG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSN 355
           KGV+HRA VNSE TRISI+SLHSL MDEKMKPAEEL+DEQNP KYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Sgr029987 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.3e-172
Identity = 314/381 (82.41%), Postives = 332/381 (87.14%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSA 60
           MGL+GESSGSF TG+TAQEKGL+YVPNAYVIP PQRPSLSPE AIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACIHLGFF----------------------------QIINHGISKTVME 120
           +RSLAIE LRKACI LGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 180
           EAL QA EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEGVQVVAVNCYP 240
           D WPTNP +YREKMG+YT+EVRKVSLE+ EAITESLGL P YLSSKMAEGVQVVAVNCYP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYP 240

Query: 241 PCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGALQVHVGDHVEV 300
           PCPQP VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP VDG+LQVHVGDHVEV
Sbjct: 241 PCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 300

Query: 301 LSNGLYKGVIHRATVNSESTRISITSLHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFL 354
           LSNGLYKGV+HRA VNSE TRISI+SLHSL MDEKMKPAEEL+DEQNP KYKESSFNDFL
Sbjct: 301 LSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFL 360

BLAST of Sgr029987 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 196/356 (55.06%), Postives = 253/356 (71.07%), Query Frame = 0

Query: 4   KGESSGSFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAIVPIIDMASLRSNDSAQRS 63
           KGE S +F  G +AQE+ L YVP+ YV+P   +P  S    IVP ID++ L+  D  +R 
Sbjct: 3   KGEDSSTFKMGNSAQERSLPYVPDCYVVPPSSKPCDS-NSGIVPTIDVSRLKGGDDERRG 62

Query: 64  LAIEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRY 123
                            I+NHGI++ ++++AL  A  FF+LP KEK ++ S+DV   VRY
Sbjct: 63  -----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRY 122

Query: 124 GTSLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEA 183
            TSLKDG+D I+FWR+FLKHYAHPL  WI  WP NP  YREKMG++  EVRK+S+E+M A
Sbjct: 123 STSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGA 182

Query: 184 ITESLGLGPTYLSSKMAE-GVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGL 243
           ITESLGLG  YLSS+M E G+QV+ VNCYPPCP PE ALGLPPH+DYSCIT +LQ+  GL
Sbjct: 183 ITESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGL 242

Query: 244 EIMDM----ADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITS 303
           +I D       G W  VP+V G L+VH+GDHVEVLSNGLYK ++H+ T+N E TRIS+ S
Sbjct: 243 KIFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLAS 302

Query: 304 LHSLEMDEKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKIQE 355
           LHSL MD+KM    EL++++NP +YKESSFNDFL FL  ND+++G  FI+TL+I++
Sbjct: 303 LHSLGMDDKMSVPRELVNDENPVRYKESSFNDFLDFLVKNDISQGDRFIDTLRIKD 340

BLAST of Sgr029987 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.7e-95
Identity = 171/346 (49.42%), Postives = 237/346 (68.50%), Query Frame = 0

Query: 10  SFPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEI----AIVPIIDMASLRSNDSAQRSLA 69
           SF +  T    G+  VP+ YV+P  QRP+L   +      +P+ID++ L  +    RSLA
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLL--HQPFLRSLA 71

Query: 70  IEELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGT 129
           I E+  AC   GFFQ+INHGI  +V+ +AL  A +FFDLP++EKM   S +V   VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAIT 189
           SL    D++ +WR F+KHY+HPL  WID WP+NP  Y++K+G+Y      +  +++EAI+
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ESLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIM 249
           ESLGL   YL  ++ EG QV+AVNCYP CP+PE+ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DMADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEMD 309
           D  +  W  VP ++GAL V +GD VEV+SNG+YK VIHR TVN E  R+S  SLHSL + 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

Query: 310 EKMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLK 352
           +K+ PA +L++  N   Y E SFNDFL ++SSND  + + FI+T+K
Sbjct: 312 KKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQ-ERFIDTIK 353

BLAST of Sgr029987 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.5e-94
Identity = 167/345 (48.41%), Postives = 230/345 (66.67%), Query Frame = 0

Query: 11  FPTGKTAQEKGLSYVPNAYVIPAPQRPSLSPEIAI----VPIIDMASLRSNDSAQRSLAI 70
           F +     E G  +VP  Y++P  QRP L P I      +P+ID++ L  +D   R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFL--HDPLLRLCVI 72

Query: 71  EELRKACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTS 130
            E+  AC   GFFQ+INHGIS  V+++A   A  FFDLP  EKM   SD+    VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITE 190
           +    D++ +WR F+KHY+HPL +WI+ WP+NP  Y+EK+G+Y      +  +++EAI+E
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 SLGLGPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMD 250
           SLGL   YL  ++ EG QV+AVNCYP CP+PE+ALG+PPH+DY  +T +LQSS+GL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 MADGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEMDE 310
             +  W  VP ++GAL V +GD VEV+SNG+YK V+HR TVN +  R+S  SLHSL M +
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

Query: 311 KMKPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLK 352
           K+ PA +L++E  P  Y E SFNDFL ++S ND+ + K FI+TL+
Sbjct: 313 KISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQ-KRFIDTLR 353

BLAST of Sgr029987 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.3e-71
Identity = 137/344 (39.83%), Postives = 207/344 (60.17%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPQRPSLSPEIAIV--------PIIDMASLRSNDSAQRSLAIEELR 78
           E GL+ VP  Y+ P P RP L+    ++        P+ID A L       R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELL---GPNRPHVLRTIA 85

Query: 79  KACIHLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDG 138
           +AC   GFFQ++NHG+   V +  +     FF+LP +E+ KY S D+   VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKHYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGL 198
            D +  WR FLK YAHPL D++  WP++PS +R     Y +E +++   +++AI ESL +
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 -GPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSS-QGLEIMDMA 258
            G    + ++ EG QVV VNCYPPCP+PE+ LG+PPH+DY  +T +LQ   +GL+I+   
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYRD 265

Query: 259 DGTWKLVPKVDGALQVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSLHSLEMDEKM 318
           +  W  V  + G+  V+VGDH+E+ SNG YK V+HR  VNS   RIS+ SLHS  +   +
Sbjct: 266 E--WVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVV 325

Query: 319 KPAEELMDEQNPKKYKESSFNDFLKFLSSNDLAEGKSFINTLKI 353
           KP+ +L+D+ NP +Y ++ F  FL++++S +  + K+F+ + KI
Sbjct: 326 KPSPKLVDKHNPSQYMDTDFTTFLQYITSRE-PKWKNFLESRKI 363

BLAST of Sgr029987 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-60
Identity = 119/331 (35.95%), Postives = 192/331 (58.01%), Query Frame = 0

Query: 25  VPNAYVIPAPQRPSLS--PEIAIVPIIDMASLRSNDSAQRSLAIEELRKACIHLGFFQII 84
           +P  YV P   RP LS   ++   P+ID++      S  RS  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +  A EFF + ++EKMK  SDD  +  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIDFWPTNPSSYREKMGRYTREVRKVSLEVMEAITESLGLGPTYLSSKMAEG 204
            + +P+  +++ WP+NP S++E + +Y+REVR+V  ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDMADGTWKLVPKVDGAL 264
            Q +AVN YPPCP+PE+  GLP HTD + +T +LQ +    +  + DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHVGDHVEVLSNGLYKGVIHRATVNSESTRISITSL---HSLEMDEKMKPAEELMDEQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S        +    KP  E  D++ 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDLAEGKSFINTL 351
              YK+ ++ ++ K   S +L +     N L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146935.22.3e-18289.55flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa... [more]
XP_008453850.16.6e-18289.83PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protei... [more]
XP_022140813.12.1e-17583.73flavanone 3-dioxygenase 3 [Momordica charantia][more]
XP_022922584.13.5e-17584.04LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata][more]
XP_038889497.16.0e-17588.29flavanone 3-dioxygenase 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q7XR844.6e-8547.83Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1... [more]
Q7XR831.6e-8249.222-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q6YYX92.5e-6739.47Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q651B62.3e-6039.12Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni... [more]
Q9FLV02.5e-5935.95Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7TMH33.2e-18289.83Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BX863.2e-18289.83protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 S... [more]
A0A6J1CIV61.0e-17583.73flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV... [more]
A0A6J1E3T81.7e-17584.04LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JAE62.3e-17282.41LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G07480.11.5e-11055.06KAR-UP oxidoreductase 1 [more]
AT2G44800.11.7e-9549.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G60290.11.5e-9448.412-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G36690.12.3e-7139.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.11.8e-6035.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 46..157
e-value: 1.3E-23
score: 84.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 14..339
e-value: 7.5E-87
score: 293.7
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 206..300
e-value: 1.8E-21
score: 76.5
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..339
NoneNo IPR availablePANTHERPTHR47991:SF67KAR-UP OXIDOREDUCTASE 1coord: 10..339
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..337
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 199..302
score: 10.494446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr029987.1Sgr029987.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding