Sgr029732 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr029732 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.1 bits (1686), Expect = 9.5e-184
Identity = 340/479 (70.98%), Postives = 384/479 (80.17%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+LDPK+IF+DS FL  VLKSL
Sbjct: 71  MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDSDFLPTVLKSL 130

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D P S     LDYN VL M   DFTPR N ELD IS+NCT
Sbjct: 131 SQTYGNSVQFPALVLIPAHPDTPLS---EFLDYNRVLGMRFDDFTPRTNAELDAISINCT 190

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 191 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 250

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VT  VIF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 251 GGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVAG 310

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI+EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DLITS
Sbjct: 311 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFGEETVQFEDLITS 370

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KATREAF GDWYRTGDV +R+
Sbjct: 371 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 430

Query: 361 KGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR+ GE  VST+EVEAVL+SHP V EAAV+       GER CGFV
Sbjct: 431 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI-------GERLCGFV 490

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTHD 473
           KLKNG  ASAEEIVEFC  HLPEFM+PE VVFGDLP+NS GKVQKF+VRE AKA+NT++
Sbjct: 491 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNN 532

BLAST of Sgr029732 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.5 bits (1682), Expect = 2.8e-183
Identity = 339/478 (70.92%), Postives = 383/478 (80.13%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  VLKSL
Sbjct: 71  MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLKSL 130

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D PPS     LDYN VL M   DFTPR N ELD IS+NCT
Sbjct: 131 SQTYGNSIQFPALVLIPAHPDTPPS---EFLDYNRVLGMRFDDFTPRTNAELDAISINCT 190

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 191 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 250

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VTA+ IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 251 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 310

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI+EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DL TS
Sbjct: 311 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEETVQFEDLFTS 370

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KATREAF GDWYRTGDV +R+
Sbjct: 371 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 430

Query: 361 KGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR+ GE  VST++VEAVL+SHP VAEAAVV       GER CGFV
Sbjct: 431 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 490

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTH 472
           KLKNG  ASAEEIVEFC  HLPEFM+PE VVFGDLP+NS GKVQKF+VRE AK +NT+
Sbjct: 491 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTN 531

BLAST of Sgr029732 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 652.1 bits (1681), Expect = 3.6e-183
Identity = 338/478 (70.71%), Postives = 383/478 (80.13%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  VLKSL
Sbjct: 71  MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLKSL 130

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIP   D PPS     LDYN VL M   DFTPR N ELD IS+NCT
Sbjct: 131 SQTYGNSIQFPALVLIPTHPDTPPS---EFLDYNRVLGMRFDDFTPRTNAELDAISINCT 190

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 191 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 250

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VTA+ IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 251 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 310

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI+EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E + +F+DL TS
Sbjct: 311 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEEIVQFEDLFTS 370

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KATREAF GDWYRTGDV +R+
Sbjct: 371 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 430

Query: 361 KGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR+ GE  VST++VEAVL+SHP VAEAAVV       GER CGFV
Sbjct: 431 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 490

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTH 472
           KLKNG  ASAEEIVEFC  HLPEFM+PE VVFGDLP+NS GKVQKF+VRE AK +NT+
Sbjct: 491 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTN 531

BLAST of Sgr029732 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 651.4 bits (1679), Expect = 6.1e-183
Identity = 338/478 (70.71%), Postives = 384/478 (80.33%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  VL+SL
Sbjct: 119 MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLQSL 178

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D PPS     LDYN VL M   DFTPR N ELD IS+NCT
Sbjct: 179 SQTYGNSIQFPALVLIPAHPDTPPS---EFLDYNRVLGMRFDDFTPRTNAELDAISINCT 238

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 239 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 298

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VT + IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 299 GGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVAG 358

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI+EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DLITS
Sbjct: 359 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEETVQFEDLITS 418

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KATREAF GDWYRTGDV +R+
Sbjct: 419 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 478

Query: 361 KGGSIEMKDRAKDIIVRSGGEV-VSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR+ GEV VST++VEAVL+SHP VAEAAVV       GER CGFV
Sbjct: 479 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 538

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTH 472
           KLKNG  ASAEEIV+FC  HLPEFM+PE VVFGDLP+NS GKVQKF+ RE AKA+NT+
Sbjct: 539 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 579

BLAST of Sgr029732 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 637.9 bits (1644), Expect = 7.0e-179
Identity = 330/479 (68.89%), Postives = 380/479 (79.33%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  +L+SL
Sbjct: 112 MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTLLQSL 171

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D PPS     LDYN VL M   +FTPR N ELD IS+NCT
Sbjct: 172 SQTYGNSIQFPALVLIPAHPDTPPS---EFLDYNRVLEMRFDEFTPRTNSELDAISINCT 231

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 232 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 291

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VT + IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 292 GGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVAG 351

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DLI S
Sbjct: 352 ALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEETVQFEDLIAS 411

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP++MES+  DGETLGE+MLRGN+LMSGY KN KAT EAF GDWYRTGDV +++
Sbjct: 412 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 471

Query: 361 KGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR  GE  VST++VEAVL+SHP VAEAAV+       GER CG V
Sbjct: 472 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 531

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTHD 473
           KLKNG  ASAEEIVEFC  HLPEFM+PE VVFGDLP+NS GKVQKF+ RE AKA+NT++
Sbjct: 532 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNN 573

BLAST of Sgr029732 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.9e-118
Identity = 225/476 (47.27%), Postives = 306/476 (64.29%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           +APN+P + ELH+ VPMAGA++ +LN + D+  +A+LL+    K+IF D QFLQI   + 
Sbjct: 72  LAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGAC 131

Query: 61  S-QNGKSTKALAVVLIP---ADSDDPPSLPEAPLDYNGVLAMGSADF-TPRPNGELDPIS 120
              + K  K   +VLIP     S       E  ++Y  V+AMG +DF   RP  E D IS
Sbjct: 132 EILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAIS 191

Query: 121 VNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCNGWCFVWAVA 180
           VN TSG+T   KGVVYSHR  YLNSLA +  +      P +LWT  MF CNGWC +W V 
Sbjct: 192 VNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLN-EMHSSPTYLWTNPMFHCNGWCLLWGVT 251

Query: 181 ALGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEA-TGHRNPLPRRVDLIVAG 240
           A+GG N+CLR VTA+ IFD +  H VT + G P IL +I  A    + PLP +V  I   
Sbjct: 252 AIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGA 311

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL--I 300
           A P   ++ ++ E GF++ + YG+TE  GP  I  W+ +  S        +   Q +  +
Sbjct: 312 APPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHL 371

Query: 301 TSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAI 360
              E+ VKDP TM +LPADG T+GE++ RGNT+M+GYLKNP+AT+EAFKG W+ +GD+ +
Sbjct: 372 GLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGV 431

Query: 361 RNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGF 420
           ++  G IE+KDR+KDII+ SGGE +S++EVE+ L +HP V EAAVV + +   GE +C F
Sbjct: 432 KHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAF 491

Query: 421 VKLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
           VKLK+G  ASAEE++ +C + LP +M P S+VF DLP  STGKVQKFV+R  AKA+
Sbjct: 492 VKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 545

BLAST of Sgr029732 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.2e-118
Identity = 229/488 (46.93%), Postives = 319/488 (65.37%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQI---VL 60
           +APN+P ++ELH+AVPMAG ++  LNT+LD  TL++LL   + KI+FVD Q L+I    L
Sbjct: 117 LAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGAL 176

Query: 61  KSLSQNGKSTKALAVVLIPADSDDPPSLPEA----------PLDYNGVLAMGSADF-TPR 120
             L+++ K+ K+L +VLI   +DD  S  ++            +Y  +L  G ++F   +
Sbjct: 177 DLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIK 236

Query: 121 PNGELDPISVNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCN 180
           P  E DPIS+N TSG+T   KGVVYSHR  YLNSLAT+F        PV+LWTV MF CN
Sbjct: 237 PRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF-LHQMSVYPVYLWTVPMFHCN 296

Query: 181 GWCFVWAVAALGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEAT-GHRNPLP 240
           GWC VW VAA GG N+CLR V+ ++IF  + +H VT + G P +L +I   T     PLP
Sbjct: 297 GWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLP 356

Query: 241 RRVDLIVAGALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYL 300
            RV+++  G+ P+ +IL ++ E GFN+S+ YG+TE  GP     W+ +  S        L
Sbjct: 357 HRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKL 416

Query: 301 DEFQDL-ITSLE-VDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD 360
              Q +    LE +DVKDP TME++P DG T+GE+M RGNT+MSGY K+ +ATR+AF+GD
Sbjct: 417 KARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGD 476

Query: 361 WYRTGDVAIRNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEV 420
           W+ +GD+A++   G IE+KDR KD+I+ SGGE +S++EVE VL SH  V EAAVV + + 
Sbjct: 477 WFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQAVLEAAVVARPDH 536

Query: 421 GLGERSCGFVKLKNGCGA-SAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVR 471
             G+  CGFVKLK G      EEI+ FC +HLP +M P+++VFGD+P  STGKVQK+++R
Sbjct: 537 HWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLR 596

BLAST of Sgr029732 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.7e-109
Identity = 215/476 (45.17%), Postives = 301/476 (63.24%), Query Frame = 0

Query: 4   NIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSLSQN 63
           NIPE+YELH+AVPMAG ++ +LN + D+  ++ LL   + K+IFV+ Q L+    +L   
Sbjct: 102 NIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLL 161

Query: 64  G-KSTKALAVVLIPADSDDPPSLPEAPLD-YNGVLAMGSADF-TPRPNGELDPISVNCTS 123
             K  K   +VL+     D  S   +  D YN +LA GS DF   RP  E DPIS+N TS
Sbjct: 162 AQKDIKPPTLVLL----TDSESFTSSSYDHYNHLLANGSDDFEIRRPKNECDPISINYTS 221

Query: 124 GSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCNGWCFVWAVAALGGC 183
           G+T   K VVYSHR  YLNS+AT+          V+LW+V MF CNGWCF W  AA G  
Sbjct: 222 GTTARPKAVVYSHRGAYLNSIATVL-LHGMGTRSVYLWSVPMFHCNGWCFPWGAAAQGAT 281

Query: 184 NVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLI--SEATGHRNPLPRRVDLIVAGALPI 243
           N+C+R V+ + IFD + LH VT     P +L +I  S       PLP +V+++  G+ P 
Sbjct: 282 NICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPP 341

Query: 244 HEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL--ITSLE 303
            +++ R+ E GF +++ YG+TE  GPA     + +  +  P     L   Q L  +   E
Sbjct: 342 PKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKARQGLNHLAMEE 401

Query: 304 VDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRNKG 363
           +DV+DP +MES+ ADG T+GE+M RGNT+MSGY K+ KAT EAF+G W+RTGD+ ++++ 
Sbjct: 402 MDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRTGDLGVKHED 461

Query: 364 GSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFVKLK 423
           G I++KDR KD+++ SGGE VST+EVE VL SH  V EAAVV + +   GE  C FV LK
Sbjct: 462 GYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVTLK 521

Query: 424 NGC--GASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNT 471
            G   G SA++I++FC + LP +M P++VVF +LP  STGK+QK++++E AKAM +
Sbjct: 522 EGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAKAMGS 571

BLAST of Sgr029732 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.1e-107
Identity = 211/476 (44.33%), Postives = 299/476 (62.82%), Query Frame = 0

Query: 4   NIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSLSQN 63
           N+PE+YELH+AVPMAG ++ +LN + D+  ++ LL   + K+IFV+ Q L+    +L   
Sbjct: 102 NLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLL 161

Query: 64  G-KSTKALAVVLIPADSDDPPSLPEAPLD-YNGVLAMGSADF-TPRPNGELDPISVNCTS 123
             K  K   +VL+     D  S   +  D YN +LA GS DF   RP  E DPIS+N TS
Sbjct: 162 AQKDIKPPTLVLL----TDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYTS 221

Query: 124 GSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCNGWCFVWAVAALGGC 183
           G+T   K VVYSHR  YLNS+AT+          V+LW+V MF CNGWCF W  AA G  
Sbjct: 222 GTTARPKAVVYSHRGAYLNSIATVL-LHGMGTTSVYLWSVPMFHCNGWCFPWGAAAQGAT 281

Query: 184 NVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLI--SEATGHRNPLPRRVDLIVAGALPI 243
           N+C+R V+ + IFD + LH VT     P +L +I  S       PLP +V+++  G+ P 
Sbjct: 282 NICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPP 341

Query: 244 HEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL--ITSLE 303
            +++ R+ E GF +++ YG+TE  GPA     + +  +  P     L   Q L  +   E
Sbjct: 342 PKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHLAMEE 401

Query: 304 VDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRNKG 363
           +DV+DP TMES+ ADG T+GE+M RGNT+MSGY K+ KAT EAF+G W+R+GD+ ++++ 
Sbjct: 402 MDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRSGDLGVKHED 461

Query: 364 GSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFVKLK 423
           G I++KDR KD+++ SGGE +ST+EVE VL SH  V EAAVV + +   GE  C FV LK
Sbjct: 462 GYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVTLK 521

Query: 424 NGC--GASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNT 471
            G     SA++I++FC + LP +M P++VVF +LP  STGK+QK++++E A AM +
Sbjct: 522 EGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAMAMGS 571

BLAST of Sgr029732 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.4e-99
Identity = 203/485 (41.86%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQF---LQIVL 60
           +APN+P +YE+H++VPM GAV++ +NT+LDA T+A++L+  +PKI+FVD +F   +Q VL
Sbjct: 70  LAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPLIQEVL 129

Query: 61  KSLSQNGKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMG-----SADFTPRPNGELD 120
           + +           +++   DS   P   E  LDY G++  G     S+    R + E D
Sbjct: 130 RLIPTYQSQPHPRIILINEIDSTTKPFSKE--LDYEGLIRKGEPTPSSSASMFRVHNEHD 189

Query: 121 PISVNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGP--PVFLWTVDMFRCNGWCF 180
           PIS+N TSG+T   KGVV SH+  YL++L++I     W     PV+LWT+ MF CNGW  
Sbjct: 190 PISLNYTSGTTADPKGVVISHQGAYLSALSSII---GWEMGIFPVYLWTLPMFHCNGWTH 249

Query: 181 VWAVAALGGCNVCLRTVTAEVIFDAVELHDVT-LLCGPPAILKLISEATGHRNPLPRRVD 240
            W+VAA GG NVC+R VTA  I+  +ELH VT + C P     L+  +   ++P    V 
Sbjct: 250 TWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKSSPVQ 309

Query: 241 LIVAGALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQ 300
           ++  G+ P   ++ +V + GF++ +GYG+TEA GP +   W+ + +         L + Q
Sbjct: 310 VLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIELQQRQ 369

Query: 301 DL--ITSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRT 360
            +  +T  +VDVK+  T+ES+P DG+T+GEI+++G++LM GYLKNPKAT EAFK  W  T
Sbjct: 370 GVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHGWLNT 429

Query: 361 GDVAIRNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGE 420
           GD+ + +  G +E+KDR+KDII+ SGGE +S++EVE VL  + +V EAAVV       GE
Sbjct: 430 GDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAAVVAMPHPLWGE 489

Query: 421 RSCGFVKLK---NGCGASAEEIVEFCGEHLPEFMVPESVV-FGDLPLNSTGKVQKFVVRE 469
             C FV LK    G   S  +++++C E++P FM P+ VV F +LP NS GK+ K  +R+
Sbjct: 490 TPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSKLRD 548

BLAST of Sgr029732 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.0e-183
Identity = 338/478 (70.71%), Postives = 384/478 (80.33%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  VL+SL
Sbjct: 119 MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLQSL 178

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D PPS     LDYN VL M   DFTPR N ELD IS+NCT
Sbjct: 179 SQTYGNSIQFPALVLIPAHPDTPPS---EFLDYNRVLGMRFDDFTPRTNAELDAISINCT 238

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 239 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 298

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VT + IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 299 GGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVAG 358

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI+EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DLITS
Sbjct: 359 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEETVQFEDLITS 418

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KATREAF GDWYRTGDV +R+
Sbjct: 419 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 478

Query: 361 KGGSIEMKDRAKDIIVRSGGEV-VSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR+ GEV VST++VEAVL+SHP VAEAAVV       GER CGFV
Sbjct: 479 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 538

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTH 472
           KLKNG  ASAEEIV+FC  HLPEFM+PE VVFGDLP+NS GKVQKF+ RE AKA+NT+
Sbjct: 539 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTN 579

BLAST of Sgr029732 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 3.4e-179
Identity = 330/479 (68.89%), Postives = 380/479 (79.33%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPN+PELYELH+AVPMAGA+IS LNT+LDAPTL+LLLQ+L PK+IF+DS FL  +L+SL
Sbjct: 112 MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTLLQSL 171

Query: 61  SQN-GKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMGSADFTPRPNGELDPISVNCT 120
           SQ  G S +  A+VLIPA  D PPS     LDYN VL M   +FTPR N ELD IS+NCT
Sbjct: 172 SQTYGNSIQFPALVLIPAHPDTPPS---EFLDYNRVLEMRFDEFTPRTNSELDAISINCT 231

Query: 121 SGSTGLHKGVVYSHRATYLNSLATIFRSRSWR--GPPVFLWTVDMFRCNGWCFVWAVAAL 180
           SGSTGLHKGVVYSHRA YLNSLATIFRS   +    PVFLWTVDMFRCNGWCF+WA+AAL
Sbjct: 232 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 291

Query: 181 GGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLP---RRVDLIVAG 240
           GGCN+CLR VT + IF  +ELH VTLLCGP  +LK+I E++   N  P   RRVDLIVAG
Sbjct: 292 GGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVAG 351

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLITS 300
           ALPI EILT+VTE GFNISYGYGMTEA+GPA+IRPW+       P   E   +F+DLI S
Sbjct: 352 ALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWK-------PNFDEETVQFEDLIAS 411

Query: 301 LEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRN 360
           LE+DVKDP++MES+  DGETLGE+MLRGN+LMSGY KN KAT EAF GDWYRTGDV +++
Sbjct: 412 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 471

Query: 361 KGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFV 420
           K G IEMKDRAKDI+VR  GE  VST++VEAVL+SHP VAEAAV+       GER CG V
Sbjct: 472 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 531

Query: 421 KLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAMNTHD 473
           KLKNG  ASAEEIVEFC  HLPEFM+PE VVFGDLP+NS GKVQKF+ RE AKA+NT++
Sbjct: 532 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNN 573

BLAST of Sgr029732 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 6.0e-176
Identity = 329/477 (68.97%), Postives = 381/477 (79.87%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPNIPELYELH+AVPMAGA+IS+LNT+LD+PTL+LLLQ+L+PK+IF+DSQFL I+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 61  SQNGKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAM--GSADFTPRPNGELDPISVNC 120
             +  S K  A+VLIP+D D P  LP   LDYN VLAM  G  DFTPRPN ELDPIS+N 
Sbjct: 61  ENS--SIKFPALVLIPSDPDTP--LPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINY 120

Query: 121 TSGSTGLHKGVVYSHRATYLNSLATIFRSR--SWRGPPVFLWTVDMFRCNGWCFVWAVAA 180
           TSGSTGLHKGV+YSHRA YLNSLATIFRS+  S    PVFLWTVDMFRCNGWCF+W +AA
Sbjct: 121 TSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAA 180

Query: 181 LGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHR---NPLPRRVDLIVA 240
           LGGCN+CLRTVTA+ IF  VELH VTLLCGPP +LK+I E++ +      L RRVDLIVA
Sbjct: 181 LGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVA 240

Query: 241 GALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDLIT 300
           GALPI EILT+V E GFNISYGYGMTEA+GPAIIRPW+       P   E   +F+DLIT
Sbjct: 241 GALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK-------PTFEEDNVQFEDLIT 300

Query: 301 SLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD-WYRTGDVAI 360
           SLE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KAT EAF G+ WYRTGDV +
Sbjct: 301 SLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGV 360

Query: 361 RNKGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCG 420
           R+K G IEMKDRAKDI+VR+ GE  VST+EVE VL+SHP VAEAAVVG+  +       G
Sbjct: 361 RHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTL------YG 420

Query: 421 FVKLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
           FVKLKN    + +EIVEFC  HLPEFM+P+ +VFGDLP+NSTGKVQKF +RE  KA+
Sbjct: 421 FVKLKNRSKENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKAL 460

BLAST of Sgr029732 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 1.2e-171
Identity = 326/480 (67.92%), Postives = 377/480 (78.54%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPNIPELYELH+AVPMAG +IS+LNT+LD+PTL+LLLQ+L+PK+IF+DSQFL I+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 61  SQNGKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAM--GSADFTPRPNGELDPISVNC 120
                S K  A++LIP  SD   SLP   LDYN +L M  G  +FTPRPN ELDPIS+N 
Sbjct: 61  --ENISIKFPALILIP--SDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINY 120

Query: 121 TSGSTGLHKGVVYSHRATYLNSLATIFRSR--SWRGPPVFLWTVDMFRCNGWCFVWAVAA 180
           TSGSTGLHKGV+YSHRA YLNSLATIFRS+  S    PVFLWTVDMFRCNGWCF+W +AA
Sbjct: 121 TSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAA 180

Query: 181 LGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHR---NPLPRRVDLIVA 240
           LGGCN+CLRTVTA+ IF  VELH VTLLCGP  +LK+I E++ +      L RRVDLIVA
Sbjct: 181 LGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVA 240

Query: 241 GALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQ---D 300
           GALPI EILT+V E GFNISYGYGMTEA+GPAIIRPW+        P  E  D  Q    
Sbjct: 241 GALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK--------PSFEDQDNVQFDDH 300

Query: 301 LITSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD-WYRTGD 360
           LITSLE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KAT EAF G+ WYRTGD
Sbjct: 301 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGD 360

Query: 361 VAIRNKGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGER 420
           V +R+K G IEMKDRAKDI+VR+ GE  VST+EVE VL+SHP VAE AVVG+  +     
Sbjct: 361 VGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTL----- 420

Query: 421 SCGFVKLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
             GFVKLKNG   + +EIVEFC  HLPEFMVP+++VFGDLP+NSTGKVQKFV+RE AKA+
Sbjct: 421 -YGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462

BLAST of Sgr029732 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 1.2e-171
Identity = 326/480 (67.92%), Postives = 377/480 (78.54%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           MAPNIPELYELH+AVPMAG +IS+LNT+LD+PTL+LLLQ+L+PK+IF+DSQFL I+LKSL
Sbjct: 71  MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130

Query: 61  SQNGKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAM--GSADFTPRPNGELDPISVNC 120
                S K  A++LIP  SD   SLP   LDYN +L M  G  +FTPRPN ELDPIS+N 
Sbjct: 131 --ENISIKFPALILIP--SDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINY 190

Query: 121 TSGSTGLHKGVVYSHRATYLNSLATIFRSR--SWRGPPVFLWTVDMFRCNGWCFVWAVAA 180
           TSGSTGLHKGV+YSHRA YLNSLATIFRS+  S    PVFLWTVDMFRCNGWCF+W +AA
Sbjct: 191 TSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAA 250

Query: 181 LGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHR---NPLPRRVDLIVA 240
           LGGCN+CLRTVTA+ IF  VELH VTLLCGP  +LK+I E++ +      L RRVDLIVA
Sbjct: 251 LGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVA 310

Query: 241 GALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQ---D 300
           GALPI EILT+V E GFNISYGYGMTEA+GPAIIRPW+        P  E  D  Q    
Sbjct: 311 GALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK--------PSFEDQDNVQFDDH 370

Query: 301 LITSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD-WYRTGD 360
           LITSLE+DVKDP +MES+  DGETLGE+MLRGNTLMSGY KN KAT EAF G+ WYRTGD
Sbjct: 371 LITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGD 430

Query: 361 VAIRNKGGSIEMKDRAKDIIVRSGGE-VVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGER 420
           V +R+K G IEMKDRAKDI+VR+ GE  VST+EVE VL+SHP VAE AVVG+  +     
Sbjct: 431 VGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTL----- 490

Query: 421 SCGFVKLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
             GFVKLKNG   + +EIVEFC  HLPEFMVP+++VFGDLP+NSTGKVQKFV+RE AKA+
Sbjct: 491 -YGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 532

BLAST of Sgr029732 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-119
Identity = 225/476 (47.27%), Postives = 306/476 (64.29%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           +APN+P + ELH+ VPMAGA++ +LN + D+  +A+LL+    K+IF D QFLQI   + 
Sbjct: 72  LAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGAC 131

Query: 61  S-QNGKSTKALAVVLIP---ADSDDPPSLPEAPLDYNGVLAMGSADF-TPRPNGELDPIS 120
              + K  K   +VLIP     S       E  ++Y  V+AMG +DF   RP  E D IS
Sbjct: 132 EILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAIS 191

Query: 121 VNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCNGWCFVWAVA 180
           VN TSG+T   KGVVYSHR  YLNSLA +  +      P +LWT  MF CNGWC +W V 
Sbjct: 192 VNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLN-EMHSSPTYLWTNPMFHCNGWCLLWGVT 251

Query: 181 ALGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEA-TGHRNPLPRRVDLIVAG 240
           A+GG N+CLR VTA+ IFD +  H VT + G P IL +I  A    + PLP +V  I   
Sbjct: 252 AIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGA 311

Query: 241 ALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL--I 300
           A P   ++ ++ E GF++ + YG+TE  GP  I  W+ +  S        +   Q +  +
Sbjct: 312 APPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHL 371

Query: 301 TSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAI 360
              E+ VKDP TM +LPADG T+GE++ RGNT+M+GYLKNP+AT+EAFKG W+ +GD+ +
Sbjct: 372 GLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGV 431

Query: 361 RNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGF 420
           ++  G IE+KDR+KDII+ SGGE +S++EVE+ L +HP V EAAVV + +   GE +C F
Sbjct: 432 KHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAF 491

Query: 421 VKLKNGCGASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
           VKLK+G  ASAEE++ +C + LP +M P S+VF DLP  STGKVQKFV+R  AKA+
Sbjct: 492 VKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 545

BLAST of Sgr029732 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 426.4 bits (1095), Expect = 3.0e-119
Identity = 229/488 (46.93%), Postives = 319/488 (65.37%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQI---VL 60
           +APN+P ++ELH+AVPMAG ++  LNT+LD  TL++LL   + KI+FVD Q L+I    L
Sbjct: 117 LAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGAL 176

Query: 61  KSLSQNGKSTKALAVVLIPADSDDPPSLPEA----------PLDYNGVLAMGSADF-TPR 120
             L+++ K+ K+L +VLI   +DD  S  ++            +Y  +L  G ++F   +
Sbjct: 177 DLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIK 236

Query: 121 PNGELDPISVNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCN 180
           P  E DPIS+N TSG+T   KGVVYSHR  YLNSLAT+F        PV+LWTV MF CN
Sbjct: 237 PRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF-LHQMSVYPVYLWTVPMFHCN 296

Query: 181 GWCFVWAVAALGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEAT-GHRNPLP 240
           GWC VW VAA GG N+CLR V+ ++IF  + +H VT + G P +L +I   T     PLP
Sbjct: 297 GWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLP 356

Query: 241 RRVDLIVAGALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYL 300
            RV+++  G+ P+ +IL ++ E GFN+S+ YG+TE  GP     W+ +  S        L
Sbjct: 357 HRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKL 416

Query: 301 DEFQDL-ITSLE-VDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD 360
              Q +    LE +DVKDP TME++P DG T+GE+M RGNT+MSGY K+ +ATR+AF+GD
Sbjct: 417 KARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGD 476

Query: 361 WYRTGDVAIRNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEV 420
           W+ +GD+A++   G IE+KDR KD+I+ SGGE +S++EVE VL SH  V EAAVV + + 
Sbjct: 477 WFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQAVLEAAVVARPDH 536

Query: 421 GLGERSCGFVKLKNGCGA-SAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVR 471
             G+  CGFVKLK G      EEI+ FC +HLP +M P+++VFGD+P  STGKVQK+++R
Sbjct: 537 HWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLR 596

BLAST of Sgr029732 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 416.8 bits (1070), Expect = 2.4e-116
Identity = 221/469 (47.12%), Postives = 300/469 (63.97%), Query Frame = 0

Query: 8   LYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSLS-QNGKS 67
           + ELH+ VPMAGA++ +LN + D+  +A+LL+    K+IF D QFLQI   +    + K 
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 68  TKALAVVLIP---ADSDDPPSLPEAPLDYNGVLAMGSADF-TPRPNGELDPISVNCTSGS 127
            K   +VLIP     S       E  ++Y  V+AMG +DF   RP  E D ISVN TSG+
Sbjct: 61  DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120

Query: 128 TGLHKGVVYSHRATYLNSLATIFRSRSWRGPPVFLWTVDMFRCNGWCFVWAVAALGGCNV 187
           T   KGVVYSHR  YLNSLA +  +      P +LWT  MF CNGWC +W V A+GG N+
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLN-EMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNI 180

Query: 188 CLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEA-TGHRNPLPRRVDLIVAGALPIHEI 247
           CLR VTA+ IFD +  H VT + G P IL +I  A    + PLP +V  I   A P   +
Sbjct: 181 CLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHV 240

Query: 248 LTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL--ITSLEVDV 307
           + ++ E GF++ + YG+TE  GP  I  W+ +  S        +   Q +  +   E+ V
Sbjct: 241 IFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQV 300

Query: 308 KDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRTGDVAIRNKGGSI 367
           KDP TM +LPADG T+GE++ RGNT+M+GYLKNP+AT+EAFKG W+ +GD+ +++  G I
Sbjct: 301 KDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYI 360

Query: 368 EMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSCGFVKLKNGC 427
           E+KDR+KDII+ SGGE +S++EVE+ L +HP V EAAVV + +   GE +C FVKLK+G 
Sbjct: 361 ELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGS 420

Query: 428 GASAEEIVEFCGEHLPEFMVPESVVFGDLPLNSTGKVQKFVVRENAKAM 469
            ASAEE++ +C + LP +M P S+VF DLP  STGKVQKFV+R  AKA+
Sbjct: 421 KASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467

BLAST of Sgr029732 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 363.6 bits (932), Expect = 2.4e-100
Identity = 203/485 (41.86%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQF---LQIVL 60
           +APN+P +YE+H++VPM GAV++ +NT+LDA T+A++L+  +PKI+FVD +F   +Q VL
Sbjct: 70  LAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPLIQEVL 129

Query: 61  KSLSQNGKSTKALAVVLIPADSDDPPSLPEAPLDYNGVLAMG-----SADFTPRPNGELD 120
           + +           +++   DS   P   E  LDY G++  G     S+    R + E D
Sbjct: 130 RLIPTYQSQPHPRIILINEIDSTTKPFSKE--LDYEGLIRKGEPTPSSSASMFRVHNEHD 189

Query: 121 PISVNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGP--PVFLWTVDMFRCNGWCF 180
           PIS+N TSG+T   KGVV SH+  YL++L++I     W     PV+LWT+ MF CNGW  
Sbjct: 190 PISLNYTSGTTADPKGVVISHQGAYLSALSSII---GWEMGIFPVYLWTLPMFHCNGWTH 249

Query: 181 VWAVAALGGCNVCLRTVTAEVIFDAVELHDVT-LLCGPPAILKLISEATGHRNPLPRRVD 240
            W+VAA GG NVC+R VTA  I+  +ELH VT + C P     L+  +   ++P    V 
Sbjct: 250 TWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKSSPVQ 309

Query: 241 LIVAGALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQ 300
           ++  G+ P   ++ +V + GF++ +GYG+TEA GP +   W+ + +         L + Q
Sbjct: 310 VLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIELQQRQ 369

Query: 301 DL--ITSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGDWYRT 360
            +  +T  +VDVK+  T+ES+P DG+T+GEI+++G++LM GYLKNPKAT EAFK  W  T
Sbjct: 370 GVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHGWLNT 429

Query: 361 GDVAIRNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGE 420
           GD+ + +  G +E+KDR+KDII+ SGGE +S++EVE VL  + +V EAAVV       GE
Sbjct: 430 GDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAAVVAMPHPLWGE 489

Query: 421 RSCGFVKLK---NGCGASAEEIVEFCGEHLPEFMVPESVV-FGDLPLNSTGKVQKFVVRE 469
             C FV LK    G   S  +++++C E++P FM P+ VV F +LP NS GK+ K  +R+
Sbjct: 490 TPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSKLRD 548

BLAST of Sgr029732 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 350.5 bits (898), Expect = 2.1e-96
Identity = 197/483 (40.79%), Postives = 296/483 (61.28%), Query Frame = 0

Query: 1   MAPNIPELYELHYAVPMAGAVISSLNTQLDAPTLALLLQRLDPKIIFVDSQFLQIVLKSL 60
           + PN+P +YEL +AVPM+GA+++++N +LDA  L++LL+  + K++FVD   + +VL+++
Sbjct: 73  VGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFVDPNSISVVLEAV 132

Query: 61  SQNGKSTKALAVVLIPADSDDPPSLPEAPLD----YNGVLAMGSADFT-PRPNGELDPIS 120
           S   ++ K   +VL+  D +D    P A  D    Y GV+  G + F   RP  E  P+ 
Sbjct: 133 SFMRQNEKP-HLVLLDDDQEDGSLSPSAASDFLDTYQGVMERGDSRFKWIRPQTEWQPMI 192

Query: 121 VNCTSGSTGLHKGVVYSHRATYLNSLATIFRSRSWRGP--PVFLWTVDMFRCNGWCFVWA 180
           +N TSG+T   KGVV SHRA ++ +++++     W  P  PV+LWT+ MF  NGW + W 
Sbjct: 193 LNYTSGTTSSPKGVVLSHRAIFMLTVSSLL---DWHFPNRPVYLWTLPMFHANGWGYTWG 252

Query: 181 VAALGGCNVCLRTVTAEVIFDAVELHDVTLLCGPPAILKLISEATGHRNPLPRRVDLIVA 240
            AA+G  NVC R V A  I+D ++ H VT +C  P +L +++     R PL   V ++ A
Sbjct: 253 TAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS-RKPLKNPVQVMTA 312

Query: 241 GALPIHEILTRVTEFGFNISYGYGMTEALGPAIIRPWRLDSSSSHPPPCEYLDEFQDL-- 300
           GA P   I++R    GFN+ +GYG+TE  GP +   W+ +     P     L   Q +  
Sbjct: 313 GAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERARLKSRQGVRT 372

Query: 301 ITSLEVDVKDPATMESLPADGETLGEIMLRGNTLMSGYLKNPKATREAFKGD-WYRTGDV 360
           I   EVDV+DP T +S+  DG ++GEI+L+G ++M GY K+P+ T    + D W+ +GDV
Sbjct: 373 IGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMREDGWFYSGDV 432

Query: 361 AIRNKGGSIEMKDRAKDIIVRSGGEVVSTLEVEAVLLSHPKVAEAAVVGKYEVGLGERSC 420
            + ++ G +E+KDR+KD+I+  GGE +S+ EVE VL ++P V EAAVV K +   GE  C
Sbjct: 433 GVIHEDGYLEVKDRSKDVII-CGGENISSAEVETVLYTNPVVKEAAVVAKPDKMWGETPC 492

Query: 421 GFVKLK---NGCGASAE-EIVEFCGEHLPEFMVPESVVF-GDLPLNSTGKVQKFVVRENA 469
            FV LK   NG G   E EI EFC   LP++MVP  V+F  +LP  STGK+QKF++R+ A
Sbjct: 493 AFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKIQKFLLRQMA 549