Sgr029544 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr029544
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
Locationtig00153403: 1789641 .. 1790878 (+)
RNA-Seq ExpressionSgr029544
SyntenySgr029544
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTTCATTCCACGGCTCTCTCCCCCTCCGACCGCCACAAACACCTCCGTCTCAGTGCTATTCCGTTCACTCTGCCGCCGGCCTGCTTTTCTTTTTTCTTTTCTCCGGCCATCATCACAACATCGACCACGTCTTCATCACAGTCTTTCTCCTTCAGCACTCAGTTGAAGGTCACAGTAGAGGTATATATATATATATATATAATAACGCTATCTGAATCTGTTTTCTTCGTAAACAATTCGAAAATTTCCTCCGAAAATGAATTAAAACCATCCAGTTTTACTGATTTTCGGATTTTGATTACAGGAGGCGTCGTACAATGACGAATTGGGTTCGATTGAGGATTTTAGGTCAAGGTTCCTTCGGCATAGTGTTCTTAGCGAAACCTATTACTGGAACTCCGTACTATGTCGCCGTCAAATTAACTCCGCTCCGAAACTATTCTTCACTGTTGAAGGAAAAGCATGTTCTAAGTCACTTCATCGGATGCCCAGAAATTGTTCAATATATTGGGAGTGAGATAACCATGGAAACGGATTCCCCCCATTCTATTTGTTACAGTCTGATATTAGAGTATGCTGCTGGAGGTACGTTGGCCGACTTGATTAACCAGCAGAAGAAGTTGTCGGAGAATGAGGTGAAGGAGTATCTCCGAATGATATTGAGAGGGCTCTCCTGCATTCACGGTAAAGGGTTTGTGCATGCCGATCTCAAACCGGCTAACATTCTTGCATTCCCTCAAAATGATGGCAAGATGAAGTTGAAGATAGCCGACTTTGGGCTTTCGAAAAGCTGTGATGAAGTGGAAGAGCATTGCAGTTTCAAGCCGAGATTTAGAGGTACGCCGAAATATATGTCACCAGAATCTGTTTTTGGTGCAATCGACACTTCACTCGATGTTTGGTCCGTGGGTTGTATTTTAGTTAAAATGGTTTCTGGAAAATCAGTATGGGATGATTGCACAGATCAAGTAGAGTTGATGATAAAACTTGTGTATGAGATGAAAGTACCAAAAATACCAGAAGAATTATCGAGAGAAGGAAAAGACTTTCTTAAAAAATGCTTTGATCAAAACCCTAAACGACGATGGACAGCTGATATGTTACTCCAACATTCCTATCTTAGAGAAGAAAAGAAAGGTGACAAGATAACCCGAGTTCCAAAAGAACTTTGTTTTCCTCATCTACTATGCAAGCTTCGCCAATCAACTATTCAATCTTGTCGATCTTCTTAA

mRNA sequence

ATGAATCTTCATTCCACGGCTCTCTCCCCCTCCGACCGCCACAAACACCTCCGTCTCAGTGCTATTCCGTTCACTCTGCCGCCGGCCTGCTTTTCTTTTTTCTTTTCTCCGGCCATCATCACAACATCGACCACGAGGCGTCGTACAATGACGAATTGGGTTCGATTGAGGATTTTAGGTCAAGGTTCCTTCGGCATAGTGTTCTTAGCGAAACCTATTACTGGAACTCCGTACTATGTCGCCGTCAAATTAACTCCGCTCCGAAACTATTCTTCACTGTTGAAGGAAAAGCATGTTCTAAGTCACTTCATCGGATGCCCAGAAATTGTTCAATATATTGGGAGTGAGATAACCATGGAAACGGATTCCCCCCATTCTATTTGTTACAGTCTGATATTAGAGTATGCTGCTGGAGGTACGTTGGCCGACTTGATTAACCAGCAGAAGAAGTTGTCGGAGAATGAGGTGAAGGAGTATCTCCGAATGATATTGAGAGGGCTCTCCTGCATTCACGGTAAAGGGTTTGTGCATGCCGATCTCAAACCGGCTAACATTCTTGCATTCCCTCAAAATGATGGCAAGATGAAGTTGAAGATAGCCGACTTTGGGCTTTCGAAAAGCTGTGATGAAGTGGAAGAGCATTGCAGTTTCAAGCCGAGATTTAGAGGTACGCCGAAATATATGTCACCAGAATCTGTTTTTGGTGCAATCGACACTTCACTCGATGTTTGGTCCGTGGGTTGTATTTTAGTTAAAATGGTTTCTGGAAAATCAGTATGGGATGATTGCACAGATCAAGTAGAGTTGATGATAAAACTTGTGTATGAGATGAAAGTACCAAAAATACCAGAAGAATTATCGAGAGAAGGAAAAGACTTTCTTAAAAAATGCTTTGATCAAAACCCTAAACGACGATGGACAGCTGATATGTTACTCCAACATTCCTATCTTAGAGAAGAAAAGAAAGGTGACAAGATAACCCGAGTTCCAAAAGAACTTTGTTTTCCTCATCTACTATGCAAGCTTCGCCAATCAACTATTCAATCTTGTCGATCTTCTTAA

Coding sequence (CDS)

ATGAATCTTCATTCCACGGCTCTCTCCCCCTCCGACCGCCACAAACACCTCCGTCTCAGTGCTATTCCGTTCACTCTGCCGCCGGCCTGCTTTTCTTTTTTCTTTTCTCCGGCCATCATCACAACATCGACCACGAGGCGTCGTACAATGACGAATTGGGTTCGATTGAGGATTTTAGGTCAAGGTTCCTTCGGCATAGTGTTCTTAGCGAAACCTATTACTGGAACTCCGTACTATGTCGCCGTCAAATTAACTCCGCTCCGAAACTATTCTTCACTGTTGAAGGAAAAGCATGTTCTAAGTCACTTCATCGGATGCCCAGAAATTGTTCAATATATTGGGAGTGAGATAACCATGGAAACGGATTCCCCCCATTCTATTTGTTACAGTCTGATATTAGAGTATGCTGCTGGAGGTACGTTGGCCGACTTGATTAACCAGCAGAAGAAGTTGTCGGAGAATGAGGTGAAGGAGTATCTCCGAATGATATTGAGAGGGCTCTCCTGCATTCACGGTAAAGGGTTTGTGCATGCCGATCTCAAACCGGCTAACATTCTTGCATTCCCTCAAAATGATGGCAAGATGAAGTTGAAGATAGCCGACTTTGGGCTTTCGAAAAGCTGTGATGAAGTGGAAGAGCATTGCAGTTTCAAGCCGAGATTTAGAGGTACGCCGAAATATATGTCACCAGAATCTGTTTTTGGTGCAATCGACACTTCACTCGATGTTTGGTCCGTGGGTTGTATTTTAGTTAAAATGGTTTCTGGAAAATCAGTATGGGATGATTGCACAGATCAAGTAGAGTTGATGATAAAACTTGTGTATGAGATGAAAGTACCAAAAATACCAGAAGAATTATCGAGAGAAGGAAAAGACTTTCTTAAAAAATGCTTTGATCAAAACCCTAAACGACGATGGACAGCTGATATGTTACTCCAACATTCCTATCTTAGAGAAGAAAAGAAAGGTGACAAGATAACCCGAGTTCCAAAAGAACTTTGTTTTCCTCATCTACTATGCAAGCTTCGCCAATCAACTATTCAATCTTGTCGATCTTCTTAA

Protein sequence

MNLHSTALSPSDRHKHLRLSAIPFTLPPACFSFFFSPAIITTSTTRRRTMTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEIVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKYMSPESVFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGDKITRVPKELCFPHLLCKLRQSTIQSCRSS
Homology
BLAST of Sgr029544 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-109
Identity = 201/299 (67.22%), Postives = 236/299 (78.93%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEI 109
           MTNWVR+  LG+GS+G VFLAKP T +  Y AVK+  L++YSSLL EK +L HF+G PEI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 110 VQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLSC 169
           VQ  G E+  E DS  S  YSL+LEYAAGGTLADLI +QKKLSEN VKEYL+MILRGLSC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 170 IHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKYMS 229
           IH  GFVH DLKPANILAFPQ DGKMKLKIADFGLS+ C++  E C+FK +FRGTP YMS
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE-CNFKLKFRGTPMYMS 180

Query: 230 PESVFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEELSRE 289
           PES++G ++T LD+WS+GCILV+MV+GK  WDDC D  +LM+KL   M+ PKIPEELS+E
Sbjct: 181 PESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKE 240

Query: 290 GKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGDKITRVPKELCFPHLLCKLRQSTIQ 349
           GKDFL+KCFD+NPKRRWTADMLLQH YL +EK+  K    P     PH+L K  QSTIQ
Sbjct: 241 GKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKF-QSTIQ 297

BLAST of Sgr029544 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 356.3 bits (913), Expect = 3.0e-94
Identity = 178/315 (56.51%), Postives = 231/315 (73.33%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPIT----GTPYYVAVKLTPLRNYSSLLKEKHVLSHFIG 109
           M NWV ++I+G+GS+G+V LAK +T       YY AVKLT L+  SSLL E+ VL HF  
Sbjct: 1   MENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQRKSSLLWEEQVLKHFKD 60

Query: 110 CPEIVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILR 169
           CPEIVQ +GSEIT        + Y+L LEYA GGTLADLI Q+KKL E+EVK+YLRMIL+
Sbjct: 61  CPEIVQCLGSEITFGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMILK 120

Query: 170 GLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEV--EEHCSFKPRFRG 229
           GLSCIH +GFVH DLKPAN+L FPQ+DGKMKLKIADFGL++ C  +  ++   +  +F+G
Sbjct: 121 GLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERCKHIVDDQDRGYLRKFKG 180

Query: 230 TPKYMSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKI 289
           TPKYMSPES+ F  ++ + D+WS+GCILVKMVSGKSVWDDC    +LM +L+ +  +P I
Sbjct: 181 TPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLLNDKNIPTI 240

Query: 290 PEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREE----KKGDKITRVPKELCFPHL 349
           P+ELS++GKDF+K+CF +N K+RWTADMLLQH YL EE    KKGD I ++ K +  PH 
Sbjct: 241 PKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDDIVKLIKAIRLPHQ 300

Query: 350 LCKLRQSTIQSCRSS 354
             K +  +  + +S+
Sbjct: 301 FFKRKAKSSNNEKSN 315

BLAST of Sgr029544 vs. NCBI nr
Match: XP_022154132.1 (mitogen-activated protein kinase kinase kinase YODA-like [Momordica charantia])

HSP 1 Score: 354.0 bits (907), Expect = 1.5e-93
Identity = 179/284 (63.03%), Postives = 220/284 (77.46%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPL-----RNYSSLLKEKHVLSHFI 109
           M NW+R++ LGQGS+G VFLAKP   +  Y A+K T L      ++SS L+EK +L  FI
Sbjct: 1   MANWIRVKPLGQGSYGEVFLAKPTADSLDYFAIKATQLPLHHCSSFSSPLREKCILECFI 60

Query: 110 GCPEIVQYIGSE-ITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMI 169
           GCPEIVQ  G E I ME DS     + LI+EYAAGG+LADLI ++KKL+ENEVK+YLRMI
Sbjct: 61  GCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLAENEVKDYLRMI 120

Query: 170 LRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLS-KSCDEVEEHCSF--KPR 229
           LRG++CIH KGFVHADLKP NILAFPQ DGKMKLKI DFGL+ +SC+E E +  +  + R
Sbjct: 121 LRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEEEAYDDYCVERR 180

Query: 230 FRGTPKYMSPESVFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP 289
           FRGTP YMSPESV+G +   LD+WSVGCI V++VSGK VWD C DQ +LM+KLV +M++P
Sbjct: 181 FRGTPIYMSPESVYGQVKAPLDIWSVGCIAVEIVSGKPVWDGCPDQ-KLMLKLVDKMEIP 240

Query: 290 KIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGD 325
           KIPEE S EGKDF+ +CFD  P+RRWTA+MLLQHSYL+EE K +
Sbjct: 241 KIPEEFSEEGKDFVGRCFDLEPERRWTAEMLLQHSYLKEENKDE 283

BLAST of Sgr029544 vs. NCBI nr
Match: XP_022152301.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-92
Identity = 175/281 (62.28%), Postives = 210/281 (74.73%), Query Frame = 0

Query: 49  TMTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPE 108
           ++  W R+ +LG+GS G VFLAKP  G  YYVAVK   +   SSL+ EK +L HF+GC E
Sbjct: 11  SLGTWTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSE 70

Query: 109 IVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLS 168
           I+Q IG EIT+E     S+CY+LILEYAAGGTL +LI +QK L ENEVKEYL+MILRGLS
Sbjct: 71  IIQCIGDEITLEES---SLCYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLS 130

Query: 169 CIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHC-------SFKPRF 228
           CIH KGFVH DLKP NILAFPQ+DGKMKLKIADFGLSK C   +          + KP+F
Sbjct: 131 CIHDKGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKF 190

Query: 229 RGTPKYMSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP 288
           +GTP YMSPESV FG+I    D+WS+GCILV+M++GK VW+ C    + + KLVYEM++P
Sbjct: 191 KGTPIYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELP 250

Query: 289 -KIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREE 321
             IPEELSREG+DFL  CFD +PKRRWTA MLLQH YL +E
Sbjct: 251 ADIPEELSREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

BLAST of Sgr029544 vs. NCBI nr
Match: XP_038896352.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 336.7 bits (862), Expect = 2.5e-88
Identity = 166/282 (58.87%), Postives = 214/282 (75.89%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEI 109
           M+NW R+++LG+GS GIVFLA P +    +VA+K+  +    SL+ E+ +L +F GCPEI
Sbjct: 1   MSNWNRVKVLGKGSHGIVFLAVPTSAPLDFVALKVVSIEYSYSLMIEETILLNFNGCPEI 60

Query: 110 VQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLSC 169
           +Q +GS I  E DS  ++ Y+L LEYA GG+L DLI +++KL E EVKEYLRMILRGL C
Sbjct: 61  IQRVGSGICFEGDSLDTVTYNLALEYATGGSLTDLIRRREKLPEVEVKEYLRMILRGLCC 120

Query: 170 IHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEE---HCSFKPRFRGTPK 229
           IH +G+VH D+KP NIL FP + GKMKLKIADFGLSK CDE EE     + KPRFRGTPK
Sbjct: 121 IHERGYVHGDIKPDNILVFPDDTGKMKLKIADFGLSKKCDEFEEVVISTNAKPRFRGTPK 180

Query: 230 YMSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP-KIPE 289
           YMSPES+ FG I+TSLD+WS+GCIL++MVSGK VW DC    +L  K+V +M +P  IP+
Sbjct: 181 YMSPESILFGMINTSLDIWSLGCILIEMVSGKQVWSDCKTLGQLAKKVVNQMDLPADIPD 240

Query: 290 ELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGDKI 327
           ELSREG+DFL KC DQ  ++RWTA+MLL+H YL+EE + D++
Sbjct: 241 ELSREGRDFLSKCIDQKFEQRWTANMLLKHPYLKEEYEVDEL 282

BLAST of Sgr029544 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.8e-47
Identity = 114/280 (40.71%), Postives = 162/280 (57.86%), Query Frame = 0

Query: 53  WVRLRILGQGSFGIVFLAKPITGT---PYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEI 112
           WVR   +G G+F  V  A     +   P  +AVK T     +SL  EK VL     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 113 VQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQ--QKKLSENEVKEYLRMILRGL 172
           ++  G + T+E        ++L+LEYA+ G+LA  + +   + L E+ V+ +   +LRGL
Sbjct: 63  IRCYGEDSTVENGEE---MHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 173 SCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKY 232
             IH KGF H D+K ANIL F  NDG   +KIADFGL+   D            RGTP Y
Sbjct: 123 RHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPLY 182

Query: 233 MSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWD--DCTDQVELMIKLVYEMKVPKIPE 292
           M+PE V      ++ DVW++GC +V+M SGK+ W   + +  + L+I++    ++PKIPE
Sbjct: 183 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 242

Query: 293 ELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGD 325
            LS EGKDFL KCF ++P +RWTA+MLL HS++  + + D
Sbjct: 243 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDD 275

BLAST of Sgr029544 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 8.8e-36
Identity = 107/336 (31.85%), Postives = 168/336 (50.00%), Query Frame = 0

Query: 5   STALSPS-----DRHKHLRLSAIPFTLPPAC-----FSFFFSPAIITTSTTRRRTM---T 64
           ST  SP+     +R +  RL   P  +   C     +S   SP++  +      T+   +
Sbjct: 339 STTGSPTRRLDDNRQQSHRLPLPPLLISNTCPFSPTYSAATSPSVPRSPARAEATVSPGS 398

Query: 65  NWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLT-------PLRNYSSLLKEKHVLSHFI 124
            W + R+LG GSFG V+L             ++T          +   L +E  VLS  +
Sbjct: 399 RWKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSR-L 458

Query: 125 GCPEIVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMIL 184
               IVQY GSE   +          + LEY +GG++  L+ +  +  EN ++ Y + IL
Sbjct: 459 RHQNIVQYYGSETVDDK-------LYIYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQIL 518

Query: 185 RGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGT 244
            GL+ +H K  VH D+K ANIL  P      ++K+ADFG++K         SFK    G+
Sbjct: 519 SGLAYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMAKHITAQSGPLSFK----GS 578

Query: 245 PKYMSPESVFGAIDTSL--DVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKI 304
           P +M+PE +  +  ++L  D+WS+GC +++M + K  W    + V  M K+    ++P I
Sbjct: 579 PYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQ-YEGVPAMFKIGNSKELPDI 638

Query: 305 PEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLR 319
           P+ LS EGKDF++KC  +NP  R TA  LL H+++R
Sbjct: 639 PDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFVR 657

BLAST of Sgr029544 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 4.8e-34
Identity = 90/275 (32.73%), Postives = 146/275 (53.09%), Query Frame = 0

Query: 53  WVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHF--------- 112
           W + +ILG+G +G V+L      T    AVK   + + +S  K K+++  F         
Sbjct: 170 WQKGQILGRGGYGSVYLGLN-KDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRS 229

Query: 113 IGCPEIVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMI 172
           +    IV+Y+G+ +            S+ LEY  GG+++ L+ +    SEN +K Y + I
Sbjct: 230 LRHDNIVRYLGTSLDQS-------FLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQI 289

Query: 173 LRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRG 232
           L+GLS +H    +H D+K ANIL     D K  +K++DFG SKS   +     FK   +G
Sbjct: 290 LQGLSFLHANSIIHRDIKGANILI----DTKGIVKLSDFGCSKSFSGIVS--QFK-SMQG 349

Query: 233 TPKYMSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKI 292
           TP +M+PE +       S D+WS+GC++V+M + +  W + T+   +M  +     +P I
Sbjct: 350 TPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNI 409

Query: 293 PEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYL 318
           P  +S+E  DFL  CF ++PK R  A+ LL+H ++
Sbjct: 410 PSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Sgr029544 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 100/296 (33.78%), Postives = 156/296 (52.70%), Query Frame = 0

Query: 53   WVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSS----LLKEKHVLSHFIGCPE 112
            W++  ++G+GSFG V+L   +T T   +AVK   +  YSS    +L     L   +   +
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVT-TGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLK 1234

Query: 113  ------IVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRM 172
                  IVQY+G E      + ++I YSL LEY AGG++  LI    +  E  +K     
Sbjct: 1235 DLDHLNIVQYLGFE------NKNNI-YSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQ 1294

Query: 173  ILRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFR 232
            +L+GL+ +H KG +H D+K  N+L     DG    KI+DFG+S+   ++  +       R
Sbjct: 1295 VLKGLAYLHSKGILHRDMKADNLLL--DQDG--ICKISDFGISRKSKDIYSNSDM--TMR 1354

Query: 233  GTPKYMSPESV--FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP 292
            GT  +M+PE V         +D+WS+GCI+++M +GK  W +  + V  M K+      P
Sbjct: 1355 GTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSN-LEVVAAMFKIGKSKSAP 1414

Query: 293  KIPEE----LSREGKDFLKKCFDQNPKRRWTADMLLQHSYLR-EEKKGDKITRVPK 332
             IPE+    +S+ G++FL  CF+ NP++R TA+ LL H +    E    K TR+ K
Sbjct: 1415 PIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPFSEVNETFNFKSTRLAK 1455

BLAST of Sgr029544 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 3.1e-33
Identity = 87/281 (30.96%), Postives = 147/281 (52.31%), Query Frame = 0

Query: 52  NWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSL---------LKEKHVLSH 111
           +W + +++G+G+FG V++   +         ++    N++S          L+E+  L  
Sbjct: 68  SWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLK 127

Query: 112 FIGCPEIVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRM 171
            +  P IV+Y+G+    E D+      +++LE+  GG+++ L+ +     E+ V+ Y R 
Sbjct: 128 NLSHPNIVRYLGT--VREDDT-----LNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQ 187

Query: 172 ILRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFR 231
           +L GL  +H    +H D+K ANIL     D K  +K+ADFG SK   E+      K   +
Sbjct: 188 LLLGLEYLHNHAIMHRDIKGANILV----DNKGCIKLADFGASKQVAELATMTGAK-SMK 247

Query: 232 GTPKYMSPESVFGAIDT-SLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPK 291
           GTP +M+PE +     + S D+WSVGC +++MV+GK+ W     +V  +  +      P 
Sbjct: 248 GTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPP 307

Query: 292 IPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKK 323
           IP+ LS + KDFL KC  + P  R TA  LL+H ++  + K
Sbjct: 308 IPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHK 336

BLAST of Sgr029544 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 9.5e-110
Identity = 201/299 (67.22%), Postives = 236/299 (78.93%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEI 109
           MTNWVR+  LG+GS+G VFLAKP T +  Y AVK+  L++YSSLL EK +L HF+G PEI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 110 VQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLSC 169
           VQ  G E+  E DS  S  YSL+LEYAAGGTLADLI +QKKLSEN VKEYL+MILRGLSC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 170 IHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKYMS 229
           IH  GFVH DLKPANILAFPQ DGKMKLKIADFGLS+ C++  E C+FK +FRGTP YMS
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE-CNFKLKFRGTPMYMS 180

Query: 230 PESVFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEELSRE 289
           PES++G ++T LD+WS+GCILV+MV+GK  WDDC D  +LM+KL   M+ PKIPEELS+E
Sbjct: 181 PESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKE 240

Query: 290 GKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGDKITRVPKELCFPHLLCKLRQSTIQ 349
           GKDFL+KCFD+NPKRRWTADMLLQH YL +EK+  K    P     PH+L K  QSTIQ
Sbjct: 241 GKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKF-QSTIQ 297

BLAST of Sgr029544 vs. ExPASy TrEMBL
Match: A0A6J1DKV0 (mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia OX=3673 GN=LOC111021457 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 7.3e-94
Identity = 179/284 (63.03%), Postives = 220/284 (77.46%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPL-----RNYSSLLKEKHVLSHFI 109
           M NW+R++ LGQGS+G VFLAKP   +  Y A+K T L      ++SS L+EK +L  FI
Sbjct: 1   MANWIRVKPLGQGSYGEVFLAKPTADSLDYFAIKATQLPLHHCSSFSSPLREKCILECFI 60

Query: 110 GCPEIVQYIGSE-ITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMI 169
           GCPEIVQ  G E I ME DS     + LI+EYAAGG+LADLI ++KKL+ENEVK+YLRMI
Sbjct: 61  GCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLAENEVKDYLRMI 120

Query: 170 LRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLS-KSCDEVEEHCSF--KPR 229
           LRG++CIH KGFVHADLKP NILAFPQ DGKMKLKI DFGL+ +SC+E E +  +  + R
Sbjct: 121 LRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEEEAYDDYCVERR 180

Query: 230 FRGTPKYMSPESVFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP 289
           FRGTP YMSPESV+G +   LD+WSVGCI V++VSGK VWD C DQ +LM+KLV +M++P
Sbjct: 181 FRGTPIYMSPESVYGQVKAPLDIWSVGCIAVEIVSGKPVWDGCPDQ-KLMLKLVDKMEIP 240

Query: 290 KIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGD 325
           KIPEE S EGKDF+ +CFD  P+RRWTA+MLLQHSYL+EE K +
Sbjct: 241 KIPEEFSEEGKDFVGRCFDLEPERRWTAEMLLQHSYLKEENKDE 283

BLAST of Sgr029544 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 6.2e-93
Identity = 175/281 (62.28%), Postives = 210/281 (74.73%), Query Frame = 0

Query: 49  TMTNWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPE 108
           ++  W R+ +LG+GS G VFLAKP  G  YYVAVK   +   SSL+ EK +L HF+GC E
Sbjct: 11  SLGTWTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSE 70

Query: 109 IVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEYLRMILRGLS 168
           I+Q IG EIT+E     S+CY+LILEYAAGGTL +LI +QK L ENEVKEYL+MILRGLS
Sbjct: 71  IIQCIGDEITLEES---SLCYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLS 130

Query: 169 CIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHC-------SFKPRF 228
           CIH KGFVH DLKP NILAFPQ+DGKMKLKIADFGLSK C   +          + KP+F
Sbjct: 131 CIHDKGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKF 190

Query: 229 RGTPKYMSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVP 288
           +GTP YMSPESV FG+I    D+WS+GCILV+M++GK VW+ C    + + KLVYEM++P
Sbjct: 191 KGTPIYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELP 250

Query: 289 -KIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREE 321
             IPEELSREG+DFL  CFD +PKRRWTA MLLQH YL +E
Sbjct: 251 ADIPEELSREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

BLAST of Sgr029544 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 6.0e-88
Identity = 173/291 (59.45%), Postives = 213/291 (73.20%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPI--------TGTPYYVAVKLTPLRNYSSLLKEKHVLS 109
           M NWV +++LGQGS+G+V LAK          +  PYY AVKL+PL++ SSLL E+ VL 
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 110 HFIGCPEIVQYIGSEITMETD--SPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEY 169
            F  CPEIVQY+GSEIT   D  + H   Y+L LEYAAGGTL DLI Q+ KL E EVK+Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 170 LRMILRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSC-----DEVEEH 229
           LRMIL+GLSCIH KGFVH DLKP NILAFPQ+DGKMKLKIADFG ++       D+ +++
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 230 --CSFKPRFRGTPKYMSPES-VFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMI 289
             C    +F+GTP+YMSPES VF  ++ + D+WS+GCILVKM+SGKS WDDC D  +LMI
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 290 KLVYEMKVPKIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKK 323
           KL+    +P IP ELS +GKDF+KKCF +N K RWTA+MLLQH YL EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Sgr029544 vs. ExPASy TrEMBL
Match: A0A1S3CQQ0 (mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656 GN=LOC103503238 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 3.0e-87
Identity = 172/291 (59.11%), Postives = 212/291 (72.85%), Query Frame = 0

Query: 50  MTNWVRLRILGQGSFGIVFLAKPIT--------GTPYYVAVKLTPLRNYSSLLKEKHVLS 109
           M NWV +++LGQGS+ +V LAK  +          PYY AVKL+PL++ SSLL E+ VL 
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 110 HFIGCPEIVQYIGSEITMETD--SPHSICYSLILEYAAGGTLADLINQQKKLSENEVKEY 169
            F  CPEIVQY+GSEIT   D  + H   Y+L LEYAAGGTL DLI Q+ KL E EVK+Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 170 LRMILRGLSCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSC-----DEVEEH 229
           LRMIL+GLSCIH KGFVH DLKP NILAFPQ+DGKMKLKIADFG ++       D+ +++
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 230 --CSFKPRFRGTPKYMSPES-VFGAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMI 289
             C    +F+GTP+YMSPES VF  ++ + D+WS+GCILVKM+SGKS WDDC D  +LMI
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 290 KLVYEMKVPKIPEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKK 323
           KL+    +P IP ELS +GKDF+KKCF +N K RWTA+MLLQH YL EE +
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENE 291

BLAST of Sgr029544 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-51
Identity = 115/292 (39.38%), Postives = 170/292 (58.22%), Query Frame = 0

Query: 56  LRILGQGSFGIVFLAK--PITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEIVQYI 115
           ++ LG+G++  V L K     G+ ++ AVK +   N  SLLKE H+LS   GCP I+Q  
Sbjct: 8   VKFLGEGAYSFVDLFKYTKSDGSSFHAAVKSSDDEN--SLLKEFHILSELKGCPRIIQCF 67

Query: 116 GSEITMETDSPHSICYSLILEYAAGGTLADLINQ--QKKLSENEVKEYLRMILRGLSCIH 175
           G+++    D   +  Y L+LEYA+ G+L+D +N    +KL +  ++++ RMIL+GL  IH
Sbjct: 68  GNDLEEGFDDKGNRVYKLLLEYASEGSLSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIH 127

Query: 176 GKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKYMSPE 235
             G+VH DLKP N+L FP  D   ++KI+DFGLS    EV +H   +  F GT  YM PE
Sbjct: 128 SHGYVHCDLKPENVLVFPCGD-SYEVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPE 187

Query: 236 SVF-GAIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEELSREG 295
           S+  G  + +LD+WS+GC++++M   K  W       E  + ++     P+IPE L  + 
Sbjct: 188 SLHDGVANKTLDLWSLGCLVLEMYVCKKPWIGFIP--EDFVYILSNGNPPEIPESLPCDA 247

Query: 296 KDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGDKITRVPKELCFPHLLCKL 343
           + F++KCF +NPK R TA  LL H +LR+EK   K+      L F   L KL
Sbjct: 248 RAFIQKCFSRNPKERGTASELLSHRFLRQEKSKLKMISPFNLLKFKKFLNKL 294

BLAST of Sgr029544 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 4.2e-49
Identity = 112/282 (39.72%), Postives = 162/282 (57.45%), Query Frame = 0

Query: 56  LRILGQGSFGIV--FLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEIVQYI 115
           +++LG+G++G V  F  K   G+  Y AVK+    NY S+ +E  +LS   GCP IVQ  
Sbjct: 8   VKVLGKGTYGSVELFSHKQNDGSLLYNAVKIMDSENYGSIDQEFRILSELRGCPCIVQLC 67

Query: 116 GSEITMETDSPHSICYSLILEYAAGGTLADLINQQK-KLSENEVKEYLRMILRGLSCIHG 175
           G+ +    D      Y + +EYAA GTL + I + + KLS++ +K++ RMIL+GL  IH 
Sbjct: 68  GNSLVQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLVSIHN 127

Query: 176 KGFVHADLKPANILAFPQNDG-----KMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKY 235
            G+VH DLKP NIL FP  D        +LKI+DFG+S    +          + GT  Y
Sbjct: 128 HGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRAGDKSGCWRVDEPWVGTSIY 187

Query: 236 MSPESVFG--AIDTSLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEE 295
           MSPESV     ++ +LD+WS+GCI++KM +GK  W      V+    L+   K P+IPE 
Sbjct: 188 MSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPWLGFEKDVK---SLLLNQKAPEIPET 247

Query: 296 LSREGKDFLKKCFDQNPKRRWTADMLLQHSYLR-EEKKGDKI 327
           L  + + FL+KCF + P+ R +A  LL H +L  +EKKG  +
Sbjct: 248 LPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDEKKGSSV 286

BLAST of Sgr029544 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 190.3 bits (482), Expect = 2.7e-48
Identity = 114/280 (40.71%), Postives = 162/280 (57.86%), Query Frame = 0

Query: 53  WVRLRILGQGSFGIVFLAKPITGT---PYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEI 112
           WVR   +G G+F  V  A     +   P  +AVK T     +SL  EK VL     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 113 VQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQ--QKKLSENEVKEYLRMILRGL 172
           ++  G + T+E        ++L+LEYA+ G+LA  + +   + L E+ V+ +   +LRGL
Sbjct: 63  IRCYGEDSTVENGEE---MHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 173 SCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKY 232
             IH KGF H D+K ANIL F  NDG   +KIADFGL+   D            RGTP Y
Sbjct: 123 RHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPLY 182

Query: 233 MSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVWD--DCTDQVELMIKLVYEMKVPKIPE 292
           M+PE V      ++ DVW++GC +V+M SGK+ W   + +  + L+I++    ++PKIPE
Sbjct: 183 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 242

Query: 293 ELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLREEKKGD 325
            LS EGKDFL KCF ++P +RWTA+MLL HS++  + + D
Sbjct: 243 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDD 275

BLAST of Sgr029544 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 188.7 bits (478), Expect = 7.8e-48
Identity = 115/284 (40.49%), Postives = 164/284 (57.75%), Query Frame = 0

Query: 53  WVRLRILGQGSFGIVFLAKPITGT----PYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPE 112
           W+R   +G GSF  V LA     +    P  +AVK + +   ++L  E+ VL     C E
Sbjct: 3   WIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSE 62

Query: 113 IVQYIGSEITMETDSPHSICYSLILEYAAGGTLADLINQQ-KKLSENEVKEYLRMILRGL 172
           IV+  G   T+E        Y+L LEYA+GG+LAD I    + L E EV+ + R I++GL
Sbjct: 63  IVRCFGEGRTVENGEE---IYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGL 122

Query: 173 SCIHGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKY 232
             IHG GF H D+K  N+L F   D    +KI+DFGL+K      E C      RGTP Y
Sbjct: 123 CHIHGNGFTHCDIKLENVLVFGDGD----VKISDFGLAKR--RSGEVC---VEIRGTPLY 182

Query: 233 MSPESV-FGAIDTSLDVWSVGCILVKMVSGKSVW---DDCTDQV-ELMIKLVYEMKVPKI 292
           M+PESV  G  ++  D+W++GC +V+M SGK+ W   D   + V  L++++    +VP+I
Sbjct: 183 MAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRI 242

Query: 293 PEELSREGKDFLKKCFDQNPKRRWTADMLLQHSYLR-EEKKGDK 326
           P ELS EGKDF+ KCF +N   RWTA+MLL H +L  +++ G++
Sbjct: 243 PVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEE 274

BLAST of Sgr029544 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 186.8 bits (473), Expect = 3.0e-47
Identity = 107/271 (39.48%), Postives = 158/271 (58.30%), Query Frame = 0

Query: 52  NWVRLRILGQGSFGIVFLAKPITGTPYYVAVKLTPLRNYSSLLKEKHVLSHFIGCPEIVQ 111
           NW+R  I+G+GS   V L   IT +  + AVK     + + L +E+ +LS  +  P IV+
Sbjct: 5   NWIRGPIIGRGSTATVSLG--ITNSGDFFAVKSAEFSSSAFLQREQSILSK-LSSPYIVK 64

Query: 112 YIGSEITMETDSPHSICYSLILEYAAGGTLADLI-NQQKKLSENEVKEYLRMILRGLSCI 171
           YIGS +T E D    + Y+L++EY +GG+L DLI N   KL E  ++ Y R IL+GL  +
Sbjct: 65  YIGSNVTKEND---KLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYL 124

Query: 172 HGKGFVHADLKPANILAFPQNDGKMKLKIADFGLSKSCDEVEEHCSFKPRFRGTPKYMSP 231
           H +G VH D+K  N++      G    KI D G +K+ +E E        F GTP +MSP
Sbjct: 125 HDQGIVHCDVKSQNVMI-----GGEIAKIVDLGCAKTVEENE-----NLEFSGTPAFMSP 184

Query: 232 ESVFGAIDT-SLDVWSVGCILVKMVSGKSVWDDCTDQVELMIKLVYEMKVPKIPEELSRE 291
           E   G   +   DVW++GC +++M +G S W +  D V  + K+ +  + P IP  LS +
Sbjct: 185 EVARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEK 244

Query: 292 GKDFLKKCFDQNPKRRWTADMLLQHSYLREE 321
           G+DFL+KC  ++PK+RWT + LLQH +L EE
Sbjct: 245 GQDFLRKCLRKDPKQRWTVEELLQHPFLDEE 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150204.12.0e-10967.22mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_038896325.13.0e-9456.51mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_022154132.11.5e-9363.03mitogen-activated protein kinase kinase kinase YODA-like [Momordica charantia][more]
XP_022152301.11.3e-9262.28mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_038896352.12.5e-8858.87mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SND63.8e-4740.71Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q9CAD58.8e-3631.85Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Q54R824.8e-3432.73Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q013891.4e-3333.78Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str... [more]
O220403.1e-3330.96Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1D9A69.5e-11067.22mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A6J1DKV07.3e-9463.03mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia ... [more]
A0A6J1DHA96.2e-9362.28mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A5D3DR576.0e-8859.45Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma... [more]
A0A1S3CQQ03.0e-8759.11mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT5G27510.12.0e-5139.38Protein kinase superfamily protein [more]
AT2G41930.14.2e-4939.72Protein kinase superfamily protein [more]
AT3G50310.12.7e-4840.71mitogen-activated protein kinase kinase kinase 20 [more]
AT4G36950.17.8e-4840.49mitogen-activated protein kinase kinase kinase 21 [more]
AT5G55090.13.0e-4739.48mitogen-activated protein kinase kinase kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 53..317
e-value: 2.0E-70
score: 249.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 55..317
e-value: 3.8E-54
score: 183.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 53..317
score: 41.70026
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 44..348
e-value: 1.9E-64
score: 219.9
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 57..321
e-value: 7.6E-19
score: 65.3
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 128..322
e-value: 2.8E-12
score: 44.0
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 128..322
e-value: 2.8E-12
score: 44.0
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 127..308
e-value: 1.3E-6
score: 24.6
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 50..343
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 50..343
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 52..317
e-value: 2.65814E-93
score: 277.094
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 59..83
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 41..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr029544.1Sgr029544.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0004672 protein kinase activity