Sgr029220 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr029220
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Mitotic spindle checkpoint protein MAD2
Location	tig00153210: 4206311 .. 4208705 (-)
RNA-Seq Expression	Sgr029220
Synteny	Sgr029220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACCGTTGGATTCGAGTAGGGAAGTAGCCGTTGCCTCGCCACTGAAGGTTCCCTTCGGACGAACCCTCTTATCTTTTCGCGTAGCTTTTAGTCGCGTCCTGCAAATCGAAGTTCTCAAATATTCTCTTGCGTACTCGGTTTTCTCTTCCTCGATTTGCGTACTCGCTTTGAAATTAAACCCTAAATCCGTTCCTCATCTTAGAGTCCAAGAAGTGTGGGCGTCCAAGTCTGATTGTTTTTAGGTTAAAGAAGATTTGATATGGCTTCCAAAACAGCGTCTAAAGACATCATCACTCTTCGTGGTTCTGCCGCGATTGTCAGCGAGTTCTTCGGTATATGCTTCAATGCCTTCTGAAACCATTCTGATCTCAATTTTACTTTTAACTCGGTTGATTATTGATGAATAACCATGGCTGATTTATTCTTTTTGGTTGTCTTGCAATTGCTCATTCAGGGTACGCTGCGAATAGGTACGAATATCGGAAATGTTTCCTGTGTTCTTATTTTCATTTTCTGATCATGTAAATATGCATTTTGTCACCTAAAAGCTATGTCGATGGTAACTCTGTAGCATCCTCTACAATCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAATCCTTCATTACTAATCTAACTGCTCAGCTTTCTGGTAAGTTCGCTTTCTGAAAATGAAAGATTTGCACCATCCGACAGTTCAGTATTCTTTTGTTTCTCTAAAAAATTTATCTGCAGTAGAACGGAGTGTAGTATGATATATTATGCTTCCCTGTAAGTGAAAACTATATATATATTGAAAAGTTCTTGAATCGCGAAATTGGTTTCAGAATGGCTGGAAGCTGGGAAGTTACAGAGGGTTGTGCTCGTCATTATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTCAGAATTGAAACTGAAGGAGAGGTTGTCGAGCAAGGGTAACGAATTCAACCAAACCTTGTCTTCCCTTTCATAAGTTTCTCGTTTTATATCTTAGTTAGATGACCTGGTTGTGCAGTGTAATGAGGGAAAAGAGCGATAAAGAAATAATGAGAGAAATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGACGAACCCTGTAAGTGTTGGCAAAATTACCATCATATATCATATATATTTTGGGGATTGATGTAACATAATGTGATATTTTTAAGCCGGGTTTTGAATCCATGCGATGTCATGTGATCCCCCATCTTGAAATCGTTAGTTTATAGTGATGGTCACGAGAGTTTTAAAAGATATTATAAATGTGTGAGCTCTTTTGCTGATTGCTCCTTTAGTAGGAAATATGATGATGATGCTCACTCAAAGCGTTTTCTGTCTCAGGTGTATTCGATGTGTTGGCATATACTGATAAAGATCTTGCAGTTCCATTTACATGGATTGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCGTTTGATACCAAGGTGAGGAGTGAATATCGTCGAACTTATTTGCTTCAGCACATTTTGATCCACTGCAAGTAACTCAACTTTTAAATGGTGTAAATGCAGATTCACAAAGTGGACACCCTTGTGTCTTACAAGAACGATGACTGCGATGACGACTAAAACACTCCCTTTTCTTGAATTCATTGTTGGCTTCTGGTTTCTGGTTTCTGGTTTCTGGTTTCTGGATCCTCCTACTTCCAAGGTGATATTGTGAATCTTTTATTCACACTTTGTATTGTAATGTAATGTAATGTAGCAACAGTTCATGTTTGTCGTGGGAGTGAATAGAAGATGGATTCAATTAGAATTTCACCTAAATGGTTTGTTTGTTTTGTTGTAAGATTTCGGGGGGAACTTTGAACAAAATGTGACTTTTTCTTTATAAAGAAAAGATGTTTACGGTGAATGTTGTGTTGTGTGACCGAGTCTTGATGATATACAGTTTGGCAAGGAATTTCGTTGGCCAAAAATAATAACCAATTTCTTCGGTCAAACCAAAGAATTGTTTGTTTCTAAATAACGACGATCCATAATTAGAAACTTCCAAGTAAATTTATCTTGAAAAATCCAAAATTTTGACGAAAGCCAAGAGAGTTGCTGAGGAAGGAAGGAAAATAATAATGGGTTTAGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAACTCTGACCCTCCACCATCGTCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGGTCCTCCGGCAAGAGCAAATTCAGGAAGACAATCTTGAGCTTCAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAATCTTGA

mRNA sequence

ATGTTACCGTTGGATTCGAGTAGGGAAGTAGCCGTTGCCTCGCCACTGAAGGTTCCCTTCGGACGAACCCTCTTATCTTTTCGCGTAGCTTTTAGTCGCGTCCTGCAAATCGAAGTTCTCAAATATTCTCTTGCGTACTCGGTTTTCTCTTCCTCGATTTGCGTACTCGCTTTGAAATTAAACCCTAAATCCGTTCCTCATCTTAGAGTCCAAGAAGTGTGGGCGTCCAACATCCTCTACAATCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAATCCTTCATTACTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTACAGAGGGTTGTGCTCGTCATTATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTCAGAATTGAAACTGAAGGAGAGGTTGTCGAGCAAGGTGTAATGAGGGAAAAGAGCGATAAAGAAATAATGAGAGAAATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGACGAACCCTGTGTATTCGATGTGTTGGCATATACTGATAAAGATCTTGCAGTTCCATTTACATGGATTGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCGTTTGATACCAAGAGAGTTGCTGAGGAAGGAAGGAAAATAATAATGGGTTTAGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAACTCTGACCCTCCACCATCGTCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGGTCCTCCGGCAAGAGCAAATTCAGGAAGACAATCTTGAGCTTCAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAATCTTGA

Coding sequence (CDS)

ATGTTACCGTTGGATTCGAGTAGGGAAGTAGCCGTTGCCTCGCCACTGAAGGTTCCCTTCGGACGAACCCTCTTATCTTTTCGCGTAGCTTTTAGTCGCGTCCTGCAAATCGAAGTTCTCAAATATTCTCTTGCGTACTCGGTTTTCTCTTCCTCGATTTGCGTACTCGCTTTGAAATTAAACCCTAAATCCGTTCCTCATCTTAGAGTCCAAGAAGTGTGGGCGTCCAACATCCTCTACAATCGTGGACTTTATCCGGAAGAAAGTTTTGTAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAATCCTTCATTACTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTACAGAGGGTTGTGCTCGTCATTATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTCAGAATTGAAACTGAAGGAGAGGTTGTCGAGCAAGGTGTAATGAGGGAAAAGAGCGATAAAGAAATAATGAGAGAAATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGACGAACCCTGTGTATTCGATGTGTTGGCATATACTGATAAAGATCTTGCAGTTCCATTTACATGGATTGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCGTTTGATACCAAGAGAGTTGCTGAGGAAGGAAGGAAAATAATAATGGGTTTAGATGGTGGTCTGTCTTGGGCAGATCAATGGGATTACAACAACTCTGACCCTCCACCATCGTCGTCGTCGGAAAATGACAAGAAGAAGAACAAGAGCGGGTCCTCCGGCAAGAGCAAATTCAGGAAGACAATCTTGAGCTTCAAGTGGATGAAAGAATTGCGAAAGAAATCTGACAACAAATCTTGA

Protein sequence

MLPLDSSREVAVASPLKVPFGRTLLSFRVAFSRVLQIEVLKYSLAYSVFSSSICVLALKLNPKSVPHLRVQEVWASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKRVAEEGRKIIMGLDGGLSWADQWDYNNSDPPPSSSSENDKKKNKSGSSGKSKFRKTILSFKWMKELRKKSDNKS

Homology

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BLAST of Sgr029220 vs. NCBI nr
Match: KAA0034220.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 390.2 bits (1001), Expect = 1.7e-104
Identity = 206/235 (87.66%), Postives = 215/235 (91.49%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK--RVAEEGRKIIMGLDGG 254
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK  +V           DGG
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDDGG 207

Query: 255 LSWADQWDYNNSDPPPSSSSENDKKKNKSGSSGKSKFRKTILSFKWMKELRKKSD 308
           LSWADQWDY N DPPP SSS+NDKKKNK GSS KSKFRKTIL FKWMK+LRKKSD
Sbjct: 208 LSWADQWDY-NPDPPP-SSSDNDKKKNKDGSSDKSKFRKTILGFKWMKDLRKKSD 260

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BLAST of Sgr029220 vs. NCBI nr
Match: TYK15700.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 382.1 bits (980), Expect = 4.6e-102
Identity = 206/276 (74.64%), Postives = 219/276 (79.35%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------------ 254
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK                  
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDGDD 207

Query: 255 --------------RVAEEGRKIIMG-----------LDGGLSWADQWDYNNSDPPPSSS 308
                         ++    ++I+              DGGLSWADQWDY N DPPP SS
Sbjct: 208 ETRVDQVSESTQFIQILRSNQRILFSKNNNLSKIRNFADGGLSWADQWDY-NPDPPP-SS 267

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BLAST of Sgr029220 vs. NCBI nr
Match: KAG7012753.1 (Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-92
Identity = 195/250 (78.00%), Postives = 207/250 (82.80%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESF RVKKYGLPMLLTQDEGVK+FI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFARVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSKANNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKRVAEE---GRKIIMGLDG 254
           PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK    +     K     D 
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDD 207

Query: 255 GLSWADQWDYNNSDP-PPSSSSENDKKKN-------------KSGSSGKSKFRKTILSFK 308
           GLSWADQWD NN DP PP SS+EN KKKN              S SS K+ F+K   + K
Sbjct: 208 GLSWADQWD-NNPDPNPPPSSAENGKKKNIFKKKKKEKEEGGSSSSSEKNIFKKA--NLK 267

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BLAST of Sgr029220 vs. NCBI nr
Match: XP_022151752.1 (mitotic spindle checkpoint protein MAD2 [Momordica charantia])

HSP 1 Score: 313.5 bits (802), Expect = 2.0e-81
Identity = 156/162 (96.30%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           I+SKANNEVLERWNF IET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  ILSKANNEVLERWNFSIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 189

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BLAST of Sgr029220 vs. NCBI nr
Match: XP_008446088.1 (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo])

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-81
Identity = 155/162 (95.68%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK 189

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BLAST of Sgr029220 vs. ExPASy Swiss-Prot
Match: Q9LU93 (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.9e-75
Identity = 137/162 (84.57%), Postives = 152/162 (93.83%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNR +YPEESFV+VKKYGLPMLL +DE VKSF++NLT+Q+SEWLEAGKLQRVVLV
Sbjct: 29  ANSILYNRAVYPEESFVKVKKYGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLV 88

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSKA  EVLERWNFRIET+ EVV++GV REKSDKEIMREIQAIMRQ+ASS+TYLPCLDE
Sbjct: 89  IMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDE 148

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
            CVFDVLAYTD D+AVPFTWIESDPKLIANPQMVKLH FDTK
Sbjct: 149 TCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK 190

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BLAST of Sgr029220 vs. ExPASy Swiss-Prot
Match: Q9XFH3 (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.1e-70
Identity = 131/162 (80.86%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNR +YPEESF +VKKYGL MLLTQDEGVK+FI +LT+QLSEWLEAGKLQR+VLV
Sbjct: 28  ANSILYNRAVYPEESFSKVKKYGLTMLLTQDEGVKNFIASLTSQLSEWLEAGKLQRIVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSKA +EVLERWNF I T+ EVVE+G ++EKSDKEIMREIQAIMRQIAS ITYLPCLDE
Sbjct: 88  IMSKATSEVLERWNFNIVTDAEVVEKGAIKEKSDKEIMREIQAIMRQIASCITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTD D+  P TW+ESD KLI NPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQMVKLHSFDTK 189

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BLAST of Sgr029220 vs. ExPASy Swiss-Prot
Match: Q556Y9 (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 3.8e-43
Identity = 89/161 (55.28%), Postives = 114/161 (70.81%), Query Frame = 0


Query: 76  SNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVI 135
           + IL+ RGLYP ESF RV KYGLP+L+T D+ +K ++ N+  QLSEWL +G +Q++VLVI
Sbjct: 29  NTILFQRGLYPPESFTRVAKYGLPILVTNDQSLKDYLDNVLKQLSEWLLSGDVQKLVLVI 88

Query: 136 MSKANNEVLERWNFRIETEGEVVEQG-VMREKSDKEIMREIQAIMRQIASSITYLPCLDE 195
                 EVLERW F + T  ++ ++G   R+K +KEIM EIQAI+RQI +S+T+LP L  
Sbjct: 89  TDIVTKEVLERWVFDVTT--DIPKEGEAPRQKPEKEIMNEIQAIIRQITASVTFLPLLPN 148

Query: 196 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDT 236
            C FD+L YT KDLAVP  W ESDPK I N Q VKL SF T
Sbjct: 149 ACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQVKLRSFTT 187

[Close]

BLAST of Sgr029220 vs. ExPASy Swiss-Prot
Match: Q9Z1B5 (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 2.2e-38
Identity = 78/160 (48.75%), Postives = 109/160 (68.12%), Query Frame = 0


Query: 76  SNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVI 135
           ++ILY RG+YP E+F RV+KYGL +L T D  +  ++ N+  QL EWL    +Q++V+VI
Sbjct: 29  NSILYQRGIYPSETFTRVQKYGLTLLTTTDPELIKYLNNVVEQLKEWLYKCSVQKLVVVI 88

Query: 136 MSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDEP 195
            +  + EVLERW F IE +    E+GV REKS K I  EI++++RQI +++T+LP L+  
Sbjct: 89  SNIESGEVLERWQFDIECDKTAKEEGVRREKSQKAIQDEIRSVIRQITATVTFLPLLEVS 148

Query: 196 CVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDT 236
           C FD+L YTDKDL VP  W ES P+ I N + V+L SF T
Sbjct: 149 CSFDLLIYTDKDLVVPEKWEESGPQFITNCEEVRLRSFTT 188

[Close]

BLAST of Sgr029220 vs. ExPASy Swiss-Prot
Match: Q13257 (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.0e-36
Identity = 74/160 (46.25%), Postives = 107/160 (66.88%), Query Frame = 0


Query: 76  SNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVI 135
           ++ILY RG+YP E+F RV+KYGL +L+T D  +  ++ N+  QL +WL    +Q++V+VI
Sbjct: 29  NSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVI 88

Query: 136 MSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDEP 195
            +  + EVLERW F IE +    +    REKS K I  EI++++RQI +++T+LP L+  
Sbjct: 89  SNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVS 148

Query: 196 CVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDT 236
           C FD+L YTDKDL VP  W ES P+ I N + V+L SF T
Sbjct: 149 CSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTT 188

[Close]

BLAST of Sgr029220 vs. ExPASy TrEMBL
Match: A0A5A7STC2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002160 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 8.1e-105
Identity = 206/235 (87.66%), Postives = 215/235 (91.49%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK--RVAEEGRKIIMGLDGG 254
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK  +V           DGG
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDDGG 207

Query: 255 LSWADQWDYNNSDPPPSSSSENDKKKNKSGSSGKSKFRKTILSFKWMKELRKKSD 308
           LSWADQWDY N DPPP SSS+NDKKKNK GSS KSKFRKTIL FKWMK+LRKKSD
Sbjct: 208 LSWADQWDY-NPDPPP-SSSDNDKKKNKDGSSDKSKFRKTILGFKWMKDLRKKSD 260

[Close]

BLAST of Sgr029220 vs. ExPASy TrEMBL
Match: A0A5D3CWF2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001990 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 2.2e-102
Identity = 206/276 (74.64%), Postives = 219/276 (79.35%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK------------------ 254
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK                  
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDGDD 207

Query: 255 --------------RVAEEGRKIIMG-----------LDGGLSWADQWDYNNSDPPPSSS 308
                         ++    ++I+              DGGLSWADQWDY N DPPP SS
Sbjct: 208 ETRVDQVSESTQFIQILRSNQRILFSKNNNLSKIRNFADGGLSWADQWDY-NPDPPP-SS 267

[Close]

BLAST of Sgr029220 vs. ExPASy TrEMBL
Match: A0A6J1DEC0 (mitotic spindle checkpoint protein MAD2 OS=Momordica charantia OX=3673 GN=LOC111019657 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 9.6e-82
Identity = 156/162 (96.30%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           I+SKANNEVLERWNF IET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  ILSKANNEVLERWNFSIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 189

[Close]

BLAST of Sgr029220 vs. ExPASy TrEMBL
Match: A0A1S3BDR9 (mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 1.3e-81
Identity = 155/162 (95.68%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSK+NNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTK 189

[Close]

BLAST of Sgr029220 vs. ExPASy TrEMBL
Match: A0A6J1HTK5 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466640 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.6e-81
Identity = 155/162 (95.68%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNRGLYPEESF RVKKYGLPMLLTQDEGVK+FI+NLTAQLSEWLEAGKLQRVVLV
Sbjct: 28  ANSILYNRGLYPEESFARVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLV 87

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSKANNEVLERWNFRIET+GEVVEQGV REKSDKEIMREIQAIMRQIASSITYLPCLDE
Sbjct: 88  IMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDE 147

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK
Sbjct: 148 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 189

[Close]

BLAST of Sgr029220 vs. TAIR 10
Match: AT3G25980.1 (DNA-binding HORMA family protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 137/162 (84.57%), Postives = 152/162 (93.83%), Query Frame = 0


Query: 75  ASNILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLV 134
           A++ILYNR +YPEESFV+VKKYGLPMLL +DE VKSF++NLT+Q+SEWLEAGKLQRVVLV
Sbjct: 29  ANSILYNRAVYPEESFVKVKKYGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLV 88

Query: 135 IMSKANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDE 194
           IMSKA  EVLERWNFRIET+ EVV++GV REKSDKEIMREIQAIMRQ+ASS+TYLPCLDE
Sbjct: 89  IMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDE 148

Query: 195 PCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
            CVFDVLAYTD D+AVPFTWIESDPKLIANPQMVKLH FDTK
Sbjct: 149 TCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK 190

[Close]

BLAST of Sgr029220 vs. TAIR 10
Match: AT3G25980.2 (DNA-binding HORMA family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.3e-75
Identity = 136/159 (85.53%), Postives = 149/159 (93.71%), Query Frame = 0


Query: 78  ILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKSFITNLTAQLSEWLEAGKLQRVVLVIMS 137
           ILYNR +YPEESFV+VKKYGLPMLL +DE VKSF++NLT+Q+SEWLEAGKLQRVVLVIMS
Sbjct: 28  ILYNRAVYPEESFVKVKKYGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLVIMS 87

Query: 138 KANNEVLERWNFRIETEGEVVEQGVMREKSDKEIMREIQAIMRQIASSITYLPCLDEPCV 197
           KA  EVLERWNFRIET+ EVV++GV REKSDKEIMREIQAIMRQ+ASS+TYLPCLDE CV
Sbjct: 88  KATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDETCV 147

Query: 198 FDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTK 237
           FDVLAYTD D+AVPFTWIESDPKLIANPQMVKLH FDTK
Sbjct: 148 FDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTK 186

[Close]

BLAST of Sgr029220 vs. TAIR 10
Match: AT1G67670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G24405.1); Has 18 Blast hits to 18 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 18; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.9 bits (167), Expect = 7.9e-12
Identity = 37/63 (58.73%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 249 LDGGLSWADQWDYNNSDPPPSSSSEND----KKKNKSGSSGKSKFRKTILSFKWMKELRK 308
           +D G SWADQWDYNN DP PSS+ E++     KK K     KS   KT+L FKWMKELRK
Sbjct: 1   MDSGPSWADQWDYNNPDPLPSSTKEDESKKKNKKKKEEDGSKSSLGKTLLGFKWMKELRK 60

[Close]

BLAST of Sgr029220 vs. TAIR 10
Match: AT1G24405.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G67670.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 65.5 bits (158), Expect = 8.8e-11
Identity = 40/62 (64.52%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 249 LDGGLSWADQWDYNNSDPPPSSSSEND---KKKNKSGSSGKSKFRKTILSFKWMKELRKK 308
           +D GLSWADQWDY NSDPPP+SS+E+D   KKK K     KS   K IL FKWMK L KK
Sbjct: 1   MDSGLSWADQWDY-NSDPPPNSSNEDDKKKKKKKKKEDGSKSGIGKAILGFKWMK-LGKK 60

The following BLAST results are available for this feature:

BLAST of Sgr029220 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0034220.1	1.7e-104	87.66	mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa]	[more]
TYK15700.1	4.6e-102	74.64	mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa]	[more]
KAG7012753.1	2.5e-92	78.00	Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022151752.1	2.0e-81	96.30	mitotic spindle checkpoint protein MAD2 [Momordica charantia]	[more]
XP_008446088.1	2.6e-81	95.68	PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo]	[more]

BLAST of Sgr029220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU93	2.9e-75	84.57	Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ...	[more]
Q9XFH3	2.1e-70	80.86	Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1	[more]
Q556Y9	3.8e-43	55.28	Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX...	[more]
Q9Z1B5	2.2e-38	48.75	Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma...	[more]
Q13257	2.0e-36	46.25	Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD...	[more]

BLAST of Sgr029220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7STC2	8.1e-105	87.66	Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5D3CWF2	2.2e-102	74.64	Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1DEC0	9.6e-82	96.30	mitotic spindle checkpoint protein MAD2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S3BDR9	1.3e-81	95.68	mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 ...	[more]
A0A6J1HTK5	1.6e-81	95.68	mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466...	[more]

BLAST of Sgr029220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25980.1	2.1e-76	84.57	DNA-binding HORMA family protein	[more]
AT3G25980.2	1.3e-75	85.53	DNA-binding HORMA family protein	[more]
AT1G67670.1	7.9e-12	58.73	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G24405.1	8.8e-11	64.52	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036570	HORMA domain superfamily	GENE3D	3.30.900.10	HORMA domain	coord: 67..240 e-value: 2.1E-61 score: 208.9
IPR036570	HORMA domain superfamily	SUPERFAMILY	56019	The spindle assembly checkpoint protein mad2	coord: 72..236
IPR003511	HORMA domain	PFAM	PF02301	HORMA	coord: 72..233 e-value: 6.6E-16 score: 58.6
IPR003511	HORMA domain	PROSITE	PS50815	HORMA	coord: 61..247 score: 35.883484
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 260..291
IPR045091	Mad2-like	PANTHER	PTHR11842	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2	coord: 76..236
IPR027097	Mitotic spindle checkpoint protein Mad2	PANTHER	PTHR11842:SF11	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A	coord: 76..236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr029220.1	Sgr029220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005654	nucleoplasm
molecular_function	GO:0003677	DNA binding