Sgr029085 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr029085 vs. NCBI nr
Match: TYK15529.1 (alpha-amylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 2378.6 bits (6163), Expect = 0.0e+00
Identity = 1157/1419 (81.54%), Postives = 1228/1419 (86.54%), Query Frame = 0

Query: 8    MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
            MS+I LDPL YHC K + RFH RPRFN LK CSF+YCPNKLLCHGR++F +YNSYRLP  
Sbjct: 1    MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 68   RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
            +A++TNA    TF+S DVL KETFP+KR EK+EGRI V+LAQGK+  +WELTVGCNL GK
Sbjct: 61   KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 128  WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
            WILHWGVSLI DSGSEWDQPPKEMIPPGSI IKDYAIETPLKKSSSSS GD VHEVKIDL
Sbjct: 121  WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 188  VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
             P+K I+AINFVLKDEETG+W+QHKGRDFKVP  DY G+DGNIVG K+ LGLW       
Sbjct: 181  APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 248  GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
                       GALGQLSNLLVK E+ SK Q SS ESGDTKE+KK LEGFYEELPIVKEV
Sbjct: 241  -----------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEV 300

Query: 308  AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
            AVDNS+SVSV+K                           D+TRKWEIPAAPHPPETTVFK
Sbjct: 301  AVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFK 360

Query: 368  NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
            NKALRT LQP EG KGC G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP  SS NLP
Sbjct: 361  NKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLP 420

Query: 428  DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
            DQQR+S+L+++G S K+GE SEGVS  AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+E+
Sbjct: 421  DQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQEN 480

Query: 488  ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
            ILQEIEKLAAEAYSIFRS+APTFTE  +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481  ILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESH 540

Query: 548  KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
            KSGRWYM LKEKAAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541  KSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600

Query: 608  KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
            KTFHDVGI VLGD VLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601  KTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660

Query: 668  DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
            DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661  DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720

Query: 728  AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
            AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721  AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780

Query: 788  LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
            LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY+LTHPGTPSVF+
Sbjct: 781  LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFY 840

Query: 848  DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 907
            DHIFSHY SEIAAL+SLRKRNKVNCRSVVKI KAERDVYAATIDE VA+KIGPG+FEPPS
Sbjct: 841  DHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPS 900

Query: 908  GSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHY 967
            GSSGWSLV EG DY K EDNAAIGL IGDLYCSI   +  R    PIT  R         
Sbjct: 901  GSSGWSLVIEGKDY-KTEDNAAIGLCIGDLYCSICWLNPTRTFHQPITMAR--------- 960

Query: 968  TILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAVAIANADIGVFDDYWKARAI 1027
                                          + LLLTLSL    A ADIG FDDYWKARA 
Sbjct: 961  ------------------------------LSLLLTLSLLATTAFADIGDFDDYWKARAA 1020

Query: 1028 EAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1087
            EA++AAA A+EP+PE + DSLNAEVGQ L+GSNSTRRNLKKYTGPCRATNPIDRCWRCDP
Sbjct: 1021 EAEQAAADAYEPNPETIADSLNAEVGQALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1080

Query: 1088 NWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWII 1147
            NWA NRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDL+NPKPGTLRHAVIQKGPLWI 
Sbjct: 1081 NWAQNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIT 1140

Query: 1148 FDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGG 1207
            F  NMAIRLTQELI+TSDKTIDARGANVQIAYGAGITLQ++ NVIIHGLRIHHIVVG GG
Sbjct: 1141 FSTNMAIRLTQELIVTSDKTIDARGANVQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGG 1200

Query: 1208 TIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFT 1267
             IRD+VDHVGLRTMSDGDGISIFGSSN+WIDHVSMSNC DGLIDAIMGSTAITISNCHFT
Sbjct: 1201 MIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFT 1260

Query: 1268 HHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1327
            HHNEVMLFGASD YS D IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI
Sbjct: 1261 HHNEVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1320

Query: 1328 GGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGD 1387
            GGSQHPTI+SQGNRFIAPPNVYAKEVTKREYSPE VWKSWTWRSEGDLMMNGAFFVTSGD
Sbjct: 1321 GGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGD 1359

Query: 1388 QRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
            QRK RPFS  DMISYKPGT+V RMTRF+GAL CFVGRPC
Sbjct: 1381 QRKRRPFSSKDMISYKPGTYVKRMTRFSGALACFVGRPC 1359

BLAST of Sgr029085 vs. NCBI nr
Match: RXH69880.1 (hypothetical protein DVH24_007136 [Malus domestica])

HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 906/1469 (61.67%), Postives = 1061/1469 (72.23%), Query Frame = 0

Query: 8    MSTIGLDPL---FYHCGKWEKRFHQRPRFN-TLK-TCSFSYCPNKLLCHGRRSFFNYNSY 67
            MST+ ++PL    +H G+ +K  H+RP+ N  LK + SF+  P KL+    RSF  +   
Sbjct: 13   MSTVRIEPLLQDLHHYGR-QKPSHRRPQSNHPLKLSSSFTAFPKKLVVSNSRSFCYFQP- 72

Query: 68   RLPAP--------RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQK 127
              P P        RA+ST+   +ET +S D + K+TFP+KRTE VEG+IFV+L  GKN+K
Sbjct: 73   --PTPRRGPTLGVRAASTDTTTVETSESADPIYKKTFPLKRTEVVEGKIFVKLDHGKNEK 132

Query: 128  QWELTVGCNLTGKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------- 187
            +W LTVGCNL GKW+LHWGVS + D   EW+QPP EM P GS+PIK              
Sbjct: 133  KWVLTVGCNLPGKWVLHWGVSFVDDVSCEWEQPPSEMRPAGSVPIKVCNWVLKFDCRAVG 192

Query: 188  -----------DYAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWF 247
                       DYAIETPLK+S SS  GDT +EVKID+ PN AI+AINFVLKDEETG W+
Sbjct: 193  WNEFDIHEDFQDYAIETPLKESLSSVGGDTSYEVKIDVKPNSAIAAINFVLKDEETGAWY 252

Query: 248  QHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLV 307
            QH+G DF+VP   Y  DD N+VG  +  G+W                  G LG+LSN+ V
Sbjct: 253  QHRGSDFRVPLVAYPQDDDNVVGATKGFGMWP-----------------GVLGKLSNVFV 312

Query: 308  KAEST-SKSQESSG-ESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK-------- 367
            KAES+ SK Q+SSG ES   +++   +EGFYEELPI KE++V+NSV+VSV K        
Sbjct: 313  KAESSNSKDQDSSGSESRHPQQKTTCVEGFYEELPIAKEISVNNSVTVSVSKCPETAKNL 372

Query: 368  -------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGI 427
                               D+T+ WEIPAAPHPPET VFK+KALRT LQ  EG  GC G+
Sbjct: 373  LYLETDLPDNAVVHWGVCRDDTKTWEIPAAPHPPETVVFKDKALRTRLQQKEGGNGCWGL 432

Query: 428  FTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEG 487
            FTLEE   GFLF+LK NE++WL   G+DFYIP SSS N    Q + + ++A V   + E 
Sbjct: 433  FTLEEGLAGFLFLLKLNESTWLRCVGNDFYIPLSSSKNANVVQSEIQSKDAQVPDGSTEA 492

Query: 488  SEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAA 547
             E     AY DG+I E+RNLV+D+ S ++ + R+K+A+E+ILQEIEKLAAEAYSIFR+  
Sbjct: 493  VE--ESTAYADGLINEMRNLVSDVFSDKSPRTRSKKAQEAILQEIEKLAAEAYSIFRTTV 552

Query: 548  PTFTEITL-ETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSL 607
            PT  E T+ ET +    P KI SGTGTGFEILCQGFNWES KSGRWYM LK KAA LSSL
Sbjct: 553  PTLPEETIAETEEVKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYMELKSKAALLSSL 612

Query: 608  GFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHR 667
            GFTVIW PPPTESVSPEGYMPRDLYNLNSRYGN+DELK+ VK FHDVGI VLGD VLNHR
Sbjct: 613  GFTVIWFPPPTESVSPEGYMPRDLYNLNSRYGNMDELKETVKVFHDVGIKVLGDAVLNHR 672

Query: 668  CAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRND 727
            CAH++NQNG+WNIFGGRLNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR D
Sbjct: 673  CAHYRNQNGVWNIFGGRLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKD 732

Query: 728  IKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHN 787
            I+EWLCWLR +IGYDGWRLDFVRGFWGGYV+DY+DASEPYFAVGEYWDSLSYTYGEMD N
Sbjct: 733  IREWLCWLRNDIGYDGWRLDFVRGFWGGYVRDYVDASEPYFAVGEYWDSLSYTYGEMDRN 792

Query: 788  QDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRA 847
            QD HRQRIVDWINATNGT GAFDVTTKGILH+AL+RCEYWRLSD+KG+PPGV+GWWPSRA
Sbjct: 793  QDAHRQRIVDWINATNGTCGAFDVTTKGILHAALERCEYWRLSDEKGKPPGVLGWWPSRA 852

Query: 848  VTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRK 907
            VTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR 
Sbjct: 853  VTFIENHDTGSTQGHWRFPKGKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRN 912

Query: 908  RNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAED 967
            RNK+NCRS+VKI KAERDVYAA IDEKVA+KIGPGH+EP SG   W+   EG DYK    
Sbjct: 913  RNKLNCRSLVKITKAERDVYAAIIDEKVAIKIGPGHYEPASGPQNWNKSLEGGDYK---- 972

Query: 968  NAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHP 1027
                 +W   L  S    S                                 G   +  P
Sbjct: 973  -----VWEASLVFSAPTRST------------------------------EDGAGGADRP 1032

Query: 1028 RKMAATTNRNMVCLL----LTLSLAVAIANADIGV--FDDYWKARAIEAQKAAATAFEPH 1087
                    ++  C              + N D  V   D YWK +A EA+K A  ++  +
Sbjct: 1033 ASAVQNRGQSAECGSGEHGFCNDNPTDVPNEDTLVLDLDAYWKEQAEEAEKVAMESYNKN 1092

Query: 1088 PEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASNRKKLA 1147
            PE VT+  N EVG++++  N+TRRNL   K YTGPC ATNP+D CWRCDPNWA+NRKK+ 
Sbjct: 1093 PEKVTEEFNTEVGKLMSKENTTRRNLRGNKAYTGPCMATNPMDACWRCDPNWANNRKKIV 1152

Query: 1148 DCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLT 1207
             C  GFG+KT GGK GP+YVV   +D D+ NP PGTLRHAVIQKGPLWIIF ++M IRL 
Sbjct: 1153 GCAQGFGKKTTGGKDGPFYVVTMGTDDDVQNPTPGTLRHAVIQKGPLWIIFTKSMVIRLQ 1212

Query: 1208 QELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVG 1267
            QEL++TSDKTIDARGANV I  GAGITLQF+ NVII  L I  IV   GG IRDSVDH+G
Sbjct: 1213 QELMVTSDKTIDARGANVVIEEGAGITLQFVKNVIITNLHIKMIVTKPGGLIRDSVDHIG 1272

Query: 1268 LRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGA 1327
            LRT SDGDGIS+FG+SN+WIDHVSMS C DGLIDAIMGSTAITISN HFT H+E MLFGA
Sbjct: 1273 LRTQSDGDGISLFGASNVWIDHVSMSRCADGLIDAIMGSTAITISNSHFTDHDEAMLFGA 1332

Query: 1328 SDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 1387
            +++++ D IMQ+T+AFNHFGQGLVQRMPR R GFFHVVNNDYTHW+MYAIGG+ +PTIIS
Sbjct: 1333 NNAHTQDKIMQITLAFNHFGQGLVQRMPRVRHGFFHVVNNDYTHWIMYAIGGNMNPTIIS 1392

Query: 1388 QGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL 1400
            QGNRFIAP N   K+VT RE +PE  WK+W WRSEGDLMMNGAFFV SG      P ++L
Sbjct: 1393 QGNRFIAPINGVTKQVTHRENTPEAEWKNWEWRSEGDLMMNGAFFVESGSGASKHP-AKL 1418

BLAST of Sgr029085 vs. NCBI nr
Match: RXH79597.1 (hypothetical protein DVH24_040744 [Malus domestica])

HSP 1 Score: 1711.4 bits (4431), Expect = 0.0e+00
Identity = 881/1468 (60.01%), Postives = 1039/1468 (70.78%), Query Frame = 0

Query: 8    MSTIGLDPLFYHCGKWEKRFHQRPRFNTLK-TCSFSYCPNKLLCHGRRSFFNYNSYRLPA 67
            MST+ ++PL +H  + +      P  + LK + SF+  P KL+    RSF N+    L +
Sbjct: 1    MSTVRIEPLLHHYRRQKPSHRLPPSKHPLKLSSSFTAFPKKLVVSNGRSFCNFQPPTL-S 60

Query: 68   PRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTG 127
             RA+ST+ A +E  +  D   KETFP+KRTE VEG++ V+L  GK+ K W LTVGCNL G
Sbjct: 61   VRAASTDTATVEATEFADAFYKETFPLKRTEVVEGKMIVKLDNGKDAKNWVLTVGCNLPG 120

Query: 128  KWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------------------D 187
            KW+LHWGV+ + D GSEWDQPP EM P GS+ IK                         D
Sbjct: 121  KWVLHWGVNYVDDVGSEWDQPPSEMRPAGSVSIKVRIWVLKFDCRAVGWHEFNKRVDFQD 180

Query: 188  YAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFS 247
            YAIETPLK+S S   GDT HEVKID+ PN AI+AINFVLKDEETG W+QH+GRDFKVPF 
Sbjct: 181  YAIETPLKESLSPVGGDTSHEVKIDVTPNSAIAAINFVLKDEETGAWYQHRGRDFKVPFV 240

Query: 248  DYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLVKAE-STSKSQES 307
             Y  DD N+VG  R+LG W                  G LG+LSN+ VKAE S SK QES
Sbjct: 241  CYLQDDDNVVGATRALGAWS-----------------GTLGKLSNVFVKAETSNSKDQES 300

Query: 308  SGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK--------------------- 367
            S ES D +++   LEGFYEELPI KE+AV++S +VSVRK                     
Sbjct: 301  SSESRDPQQKTMRLEGFYEELPIAKEIAVNHSATVSVRKCPETTKNLLYLETDLPDHAVV 360

Query: 368  ------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFV 427
                  D+ ++WEIPAAPHPPET VFK+KALRT LQ  E   GC G+FTLEE   GFLFV
Sbjct: 361  HWGVCRDDAKRWEIPAAPHPPETVVFKDKALRTRLQQREDGNGCSGLFTLEEGLAGFLFV 420

Query: 428  LKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGI 487
             K NE  WLN  G+DFYIP  SS N    Q + + E+A V  ++ E     +  AYTDGI
Sbjct: 421  FKLNETMWLNCVGNDFYIPLLSSNNSIAVQSEVQSEDAQVPDRSRE----TNFTAYTDGI 480

Query: 488  IKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAAPTF-TEITLETPK 547
            I EIRNLV+DI+S+++Q+KR+KEA+E+ILQEIEKLAAEAYSIFR+  PT   EI  ET K
Sbjct: 481  INEIRNLVSDISSEKSQRKRSKEAQETILQEIEKLAAEAYSIFRTTVPTLPEEIIAETEK 540

Query: 548  PVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTES 607
                P KI SGTGTGFEILCQGFNWES KSGRWY  LK KAAELSSLGFTVIW PPPT+S
Sbjct: 541  VKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYEELKSKAAELSSLGFTVIWFPPPTDS 600

Query: 608  VSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNI 667
            VSP+GYMPRDLYN+NSRYGN+DELK+ VKTFHD G+ VLGD VLNHRCA ++NQNG+WNI
Sbjct: 601  VSPQGYMPRDLYNMNSRYGNMDELKETVKTFHDAGLKVLGDAVLNHRCAEYQNQNGVWNI 660

Query: 668  FGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIG 727
            FGGRLNWD+RAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR DI+EWLCWLR +IG
Sbjct: 661  FGGRLNWDERAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKDIREWLCWLRDDIG 720

Query: 728  YDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWIN 787
            YDGWRLDFVRGFWGGYVKDY+DASEPYFAVGEYWDSLSYTYGEMDHNQD HRQRIVDWIN
Sbjct: 721  YDGWRLDFVRGFWGGYVKDYMDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWIN 780

Query: 788  ATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQ 847
            ATNGT GAFDVTTKGILH+AL+RCEYWRLSD KG+PPGV+GWWPSRAVTFIENHDTGSTQ
Sbjct: 781  ATNGTCGAFDVTTKGILHAALERCEYWRLSDTKGKPPGVLGWWPSRAVTFIENHDTGSTQ 840

Query: 848  GHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIE 907
            GHWRFP  KEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR RNK+NCRS VK++
Sbjct: 841  GHWRFPNKKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRNRNKLNCRSRVKLK 900

Query: 908  KAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYC 967
               +     T      MK  P   +    +    LV      K   +           + 
Sbjct: 901  SPRQ---KETSMRPSLMKRSPSKSDQVITN---LLVDLKIGIKALREETTRSGKRHKFFL 960

Query: 968  SISNPSVFRLRLN-------PITSPR-----FKAWPDNHYTILELSFLFSWGVKESLHPR 1027
                P++   R +       P ++ +      +     H +  ++S      V     P 
Sbjct: 961  HRHFPNLRDCRRSTEDETGRPASAAQNRGQSAECGSGEHVSSRKVS------VAADEVPT 1020

Query: 1028 KMAATTNRNMVCL-LLTLSLAV---------AIANADIGV--FDDYWKARAIEAQKAAAT 1087
             MAA  N +   L L  LS AV          +AN D  V   D YWK RA EA K A  
Sbjct: 1021 TMAAMFNSSYFFLCLFFLSFAVFVSSNDGPTDVANEDSTVLDLDAYWKERAQEAFKEAME 1080

Query: 1088 AFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASN 1147
            ++  +PE VT+  N  V ++L+  N+TRRNL   K Y GPC ATNPID CWRCDPNWA N
Sbjct: 1081 SYHKNPEQVTEEFNTGVAELLSKENTTRRNLRGNKGYNGPCMATNPIDACWRCDPNWADN 1140

Query: 1148 RKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNM 1207
            RKK+  C  GFG+K  GGK GP YVV   +D D+ NPKPGTLRHAVIQKGPLWIIF R+M
Sbjct: 1141 RKKIVGCAQGFGKKATGGKDGPIYVVTVGTDDDVQNPKPGTLRHAVIQKGPLWIIFARSM 1200

Query: 1208 AIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDS 1267
             IRL QEL++TSDKTID RGANV I  G GITLQF+ NVI+  L I  IV   GG IRDS
Sbjct: 1201 VIRLQQELMVTSDKTIDGRGANVVIEEGGGITLQFVKNVILTNLHIKMIVSKPGGMIRDS 1260

Query: 1268 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEV 1327
            VDH+GLRT SDGDGIS+FG+SN+WIDH+SMS C DGLIDA+M STAITISN HFT H++V
Sbjct: 1261 VDHMGLRTKSDGDGISLFGASNVWIDHMSMSRCADGLIDAVMASTAITISNSHFTDHDDV 1320

Query: 1328 MLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 1387
            MLFGA+++++ D IMQ+T+AFNHFG+GLVQRMPR R+GFFH+VNNDY HW+MYAIGG+ +
Sbjct: 1321 MLFGANNAHTQDKIMQITLAFNHFGRGLVQRMPRVRYGFFHLVNNDYNHWIMYAIGGNMN 1380

Query: 1388 PTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSR 1394
            PTIISQGNRFIAP N   K+VT RE++PE+ WK+W WRSEGDLMMNGAFFV SG +    
Sbjct: 1381 PTIISQGNRFIAPVNEATKQVTHREHTPESEWKNWEWRSEGDLMMNGAFFVESGSRASQN 1433

BLAST of Sgr029085 vs. NCBI nr
Match: RZC60748.1 (hypothetical protein C5167_022499 [Papaver somniferum])

HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 810/1378 (58.78%), Postives = 960/1378 (69.67%), Query Frame = 0

Query: 82   SKDVLLKETFPIKRTEKVEGRIFVRLAQ---GKNQKQWELTVGCNLTGKWILHWGVSLIG 141
            +++VL+ +TFP+  T++VEG+I VRL +    + +++W LTVGCNL G W+LHWGVS   
Sbjct: 56   TENVLISKTFPLNWTQRVEGKISVRLDKKDGDEAEEKWVLTVGCNLPGNWVLHWGVSYTH 115

Query: 142  DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTV-HEVKIDLVPNKAISAIN 201
            D GSEWDQPP EM PPGSIPIKDYAIETPL +SS++  G     EV+I+     +ISAIN
Sbjct: 116  DIGSEWDQPPLEMRPPGSIPIKDYAIETPLMESSAALEGQLAQQEVQIEFDTPSSISAIN 175

Query: 202  FVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFN 261
            FV KDEETGVW QH+GRDFKVP +    +D N +G K+S+G+                  
Sbjct: 176  FVFKDEETGVWCQHRGRDFKVPLTGKALNDANSIGAKKSIGILP---------------- 235

Query: 262  IGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSV 321
                      ++K  S+   +E +GE  +  ++   LE FYEE  I+KE  V ++++VSV
Sbjct: 236  ----------VLKEGSSPNVEEKTGEVEEPAQQNMRLEAFYEEYSILKEFPVQSAITVSV 295

Query: 322  RK---------------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQP 381
             K                           D  +KWEIP APHP +TT FKNKAL+T LQ 
Sbjct: 296  TKSPDSEKNLVNFETNLPGDVVVHWGVCRDSDKKWEIPEAPHPAQTTTFKNKALQTILQL 355

Query: 382  IEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEE 441
             E  +G  G+F + EE  G LFVLK + N+WLN  G+DFYIP ++  ++  Q   +E   
Sbjct: 356  KEDGRGSSGVFPVGEELMGLLFVLKLSNNTWLNDMGNDFYIPLATETSIHTQTPAAE--- 415

Query: 442  AGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAA 501
                   GEGS        TD IIKEIR+LVTDI+S++  + ++KEA+E ILQEIEKLAA
Sbjct: 416  -----TGGEGS------VDTDEIIKEIRHLVTDISSEKRWQTKSKEAQEVILQEIEKLAA 475

Query: 502  EAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLK 561
            EAY IFRS  P F E   +     + P+KI+SGTG+GFEI+CQGFNWES+KSGRWY  L 
Sbjct: 476  EAYGIFRSTTPAFVEEVADAEVLEKTPIKITSGTGSGFEIVCQGFNWESNKSGRWYKELL 535

Query: 562  EKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITV 621
            +KA EL+S+GFTVIWLPPPT+SVSPEGYMPRDLYNLNSRYG+++ELK ++K FH+VGI  
Sbjct: 536  DKANELASIGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSMEELKVLLKKFHEVGIKT 595

Query: 622  LGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNID 681
            LGD VLNHRCA  +NQNG+WNIFGG+LNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNID
Sbjct: 596  LGDAVLNHRCAQKQNQNGVWNIFGGKLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNID 655

Query: 682  HSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLS 741
            HSQ+FVRND+KEWLCWLR EIGY GWRLDFVRGFWGGYVKDY++A+EPYF+VGEYWDSLS
Sbjct: 656  HSQEFVRNDLKEWLCWLRNEIGYHGWRLDFVRGFWGGYVKDYMEATEPYFSVGEYWDSLS 715

Query: 742  YTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPG 801
            Y+YGEMDHNQD HRQRI+DWINATNGT+GAFDVTTKGILH+ L+RCEYWRLSD KG+PPG
Sbjct: 716  YSYGEMDHNQDAHRQRIIDWINATNGTSGAFDVTTKGILHATLERCEYWRLSDSKGKPPG 775

Query: 802  VVGWWPSRAVTFIENHDTGSTQ-----------------------------GHWRFPGGK 861
            VVGWWPSRAVTFIENHDTGSTQ                                +FP GK
Sbjct: 776  VVGWWPSRAVTFIENHDTGSTQVGIAYRFIAEITSCSNLIATNTDIVPFPNTLEKFPEGK 835

Query: 862  EMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAA 921
            EMQGYAYILTHPGTPSVF+DHIFSH  SEIA L+S+R RNK+NCRS + I KAERDVYAA
Sbjct: 836  EMQGYAYILTHPGTPSVFYDHIFSH-KSEIAGLISIRNRNKINCRSTINITKAERDVYAA 895

Query: 922  TIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFR 981
             IDEKVAMKIGPGH+EPP+GS  W    EG DYK  +                       
Sbjct: 896  IIDEKVAMKIGPGHYEPPNGSE-WKSAFEGRDYKTLD----------------------- 955

Query: 982  LRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAV 1041
                                                  +K+++TT R  +          
Sbjct: 956  --------------------------------------KKVSSTTLREEL---------E 1015

Query: 1042 AIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKK 1101
                A I  FDD+W  R  EA++ A   + P+PE VT   N  V   L G+NSTRR LKK
Sbjct: 1016 KQFQAHIRDFDDHWLQRQDEARRFAMEVYHPNPENVTAHFNEHVRIALEGTNSTRRTLKK 1075

Query: 1102 YTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVN 1161
            +TG C ATNPIDRCWRC PNWA++RK+LA CV GFGR T+GGK G  YVV + SD+DL N
Sbjct: 1076 HTGRCLATNPIDRCWRCRPNWATDRKRLASCVKGFGRNTVGGKFGRIYVVTNPSDNDLAN 1135

Query: 1162 PKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFI 1221
            P+PGTLRHA IQK PLWIIF R+M I L QEL++ S KTID RGANV IAYGAGIT+QF 
Sbjct: 1136 PRPGTLRHAAIQKEPLWIIFARSMLITLQQELMVQSHKTIDGRGANVHIAYGAGITVQFA 1195

Query: 1222 NNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDG 1281
             NVIIH L IH IV   GG IRDSVDH+G RT SDGD +SI+GSSN+W+DH+SMSNC DG
Sbjct: 1196 RNVIIHNLHIHDIVSRNGGLIRDSVDHIGFRTASDGDAVSIYGSSNVWVDHLSMSNCKDG 1255

Query: 1282 LIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCR 1341
            L+D I GST IT+SN H THHNEVMLFGA D    D IMQVTVAFNH+GQGL+QRMPRCR
Sbjct: 1256 LVDIIEGSTGITVSNNHMTHHNEVMLFGAHDDQPLDQIMQVTVAFNHYGQGLIQRMPRCR 1315

Query: 1342 WGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWT 1400
            WGF HVVNNDYTHWLMYAIGGSQHPTI+SQGNRFIAPP   AKEVT R  +P++VWK WT
Sbjct: 1316 WGFIHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPTQAAKEVTHRSDAPQSVWKDWT 1321

BLAST of Sgr029085 vs. NCBI nr
Match: XP_038891960.1 (alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1599.7 bits (4141), Expect = 0.0e+00
Identity = 774/915 (84.59%), Postives = 818/915 (89.40%), Query Frame = 0

Query: 8   MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
           MSTI LDPL YHCGK + RFH RPRFNTLK CSFSYCPNKLL HGR+SF NYNSYRLP  
Sbjct: 1   MSTISLDPLLYHCGKGKHRFHYRPRFNTLKPCSFSYCPNKLLHHGRKSFVNYNSYRLPTF 60

Query: 68  RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
           RAS+TNA LL+TF+SKDVL +ETFP+KR EK+EGRI VRLAQG +Q QWELTVGCNL GK
Sbjct: 61  RASATNAPLLQTFQSKDVLFQETFPLKRNEKLEGRISVRLAQGNDQNQWELTVGCNLAGK 120

Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
           WILHWGVSLI DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSS GD VHEVKI+L
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSSGDFVHEVKINL 180

Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
           VP+K I+AINFVLKDE+TG+W+QHKGRDFKVP  DYHG+DGNIVGTK+ LGLW       
Sbjct: 181 VPDKTIAAINFVLKDEQTGIWYQHKGRDFKVPLLDYHGEDGNIVGTKKGLGLWP------ 240

Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
                      GALGQLSNLLVKAES+SK +  SGESGD KEEKKYLEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKAESSSKDKGRSGESGDAKEEKKYLEGFYEELPIVKEV 300

Query: 308 AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
           AVDNSVSVSVRK                           DETRKWEIPAAPHPPETTVFK
Sbjct: 301 AVDNSVSVSVRKCPETTKNLLYLETDLPGDVIVHWGACRDETRKWEIPAAPHPPETTVFK 360

Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
           NKALRT LQP EG KGCCGIFT+EE+F GFLFVLKQNENSWLNYKGDDFYIP S+S NLP
Sbjct: 361 NKALRTLLQPKEGGKGCCGIFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLSNSGNLP 420

Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
            QQR+SEL++AGVS K+GE SEGVS  AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+ES
Sbjct: 421 GQQRESELKDAGVSKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQES 480

Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
           ILQEIEKLAAEAYSIFRS+APTFTE  +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSSAPTFTEEVIEMPKPVEPPVQISSGTGSGFEILCQGFNWESH 540

Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
           KSGRWYM LKE+AAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKERAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600

Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
           KTFHDVGI VLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660

Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
           DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720

Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
           AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780

Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
           LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTPS F+
Sbjct: 781 LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPCGKEMQGYAYILTHPGTPSAFY 840

Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 896
           DHIFSHY SEIAAL+SLRKRNKVNCRSVV IEKAERDVYAA IDEKVA+KIGPG+FEPPS
Sbjct: 841 DHIFSHYKSEIAALISLRKRNKVNCRSVVTIEKAERDVYAAIIDEKVAVKIGPGNFEPPS 898

BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/857 (66.28%), Postives = 666/857 (77.71%), Query Frame = 0

Query: 66  APRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLT 125
           A RA+S++ A++ET +S DV+ KE FP++R EK EG+I+VRL + K +K WEL+VGC++ 
Sbjct: 63  AIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVK-EKNWELSVGCSIP 122

Query: 126 GKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKI 185
           GKWILHWGVS +GD+GSEWDQPP++M PPGSI IKDYAIETPLKK S    GD+  EV I
Sbjct: 123 GKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEVAI 182

Query: 186 DLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVY 245
           +L    +++A+NFVLKDEETG W+QHKGRDFKVP  D   D+GN++G K+          
Sbjct: 183 NLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKK---------- 242

Query: 246 HVGGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVK 305
                        GALGQLSN+ +K       Q+ S    D+ EE+K L+ FYEE+PI K
Sbjct: 243 -----------GFGALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISK 302

Query: 306 EVAVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTV 365
            VA DNSVSV+ R                           K+ T+KWEIP+ P+P ET++
Sbjct: 303 RVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSL 362

Query: 366 FKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVN 425
           FKNKALRT LQ  +   G  G+F+L+ + EG  FVLK NEN+WLNY+G+DFY+PF +S +
Sbjct: 363 FKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSS 422

Query: 426 LPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAK 485
            P      E E A VS    +  + VS   +T  II EIRNL  DI+S + QK   KE +
Sbjct: 423 SP-----VETEAAQVSKPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQ 482

Query: 486 ESILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWE 545
           E+ILQEIEKLAAEAYSIFRS  P F+E  +   +  +P +KISSGTG+GFEILCQGFNWE
Sbjct: 483 ENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWE 542

Query: 546 SHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKD 605
           S+KSGRWY+ L+EKA EL+SLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYG IDELKD
Sbjct: 543 SNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKD 602

Query: 606 VVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKS 665
            VK FH VGI VLGD VLNHRCAHFKNQNG+WN+FGGRLNWDDRAVV+DDPHFQGRGNKS
Sbjct: 603 TVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKS 662

Query: 666 SGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEP 725
           SGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+P
Sbjct: 663 SGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKP 722

Query: 726 YFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEY 785
           YFAVGEYWDSLSYTYGEMD+NQD HRQRIVDWINAT+G AGAFDVTTKGILH+AL +CEY
Sbjct: 723 YFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEY 782

Query: 786 WRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSV 845
           WRLSD KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+V
Sbjct: 783 WRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAV 842

Query: 846 FFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEP 896
           FFDHIFS Y+SEIAAL+SLR R K++CRS V I+K+ERDVYAA IDEKVAMKIGPGH+EP
Sbjct: 843 FFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEP 882

BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.5e-167
Identity = 289/438 (65.98%), Postives = 332/438 (75.80%), Query Frame = 0

Query: 973  LLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVL--- 1032
            L +T +  +    A IG +D+ W+ RA EA++ A   +EPHPE VT + N ++   +   
Sbjct: 13   LCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKEL 72

Query: 1033 -----TGSNSTRRNL--KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIG 1092
                 T +NSTRR L  KKYTGPC  TNPID+CWRCDPNWA NRKKLADC +GFG K IG
Sbjct: 73   KKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIG 132

Query: 1093 GKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTID 1152
            GK G +YVV D+SD D  +PKPGTLRHAVIQK PLWIIF R M IRL QE+IM SDKTID
Sbjct: 133  GKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTID 192

Query: 1153 ARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISI 1212
            ARG NV I  GAGITLQ+I NVIIHGL IH IV G GG +RD+VDH+G+RT SDGDGISI
Sbjct: 193  ARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISI 252

Query: 1213 FGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQV 1272
            FG+S IWIDHVSM  C+DGLIDA+ GST ITISN HFT HNEVMLFGASDS S D +MQ+
Sbjct: 253  FGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQI 312

Query: 1273 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVY 1332
            T+AFNHFG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII QGNRFIAPP+++
Sbjct: 313  TLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIF 372

Query: 1333 AKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL-DMISYKPGTFV 1392
             K+VTKREY+PE+VW  WTWRSEG+L MNGA+F  SGD   S     L D IS  P   V
Sbjct: 373  KKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDV 432

Query: 1393 TRMTRFAGALPCFVGRPC 1400
            T MTRFAG L C  G+PC
Sbjct: 433  TWMTRFAGVLGCKPGKPC 449

BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.6e-155
Identity = 268/421 (63.66%), Postives = 319/421 (75.77%), Query Frame = 0

Query: 980  AVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL 1039
            + A+++A+I  FD+YW+ ++  AQ  A  A+ PHPE VT+  N  V     G NSTRRNL
Sbjct: 16   SAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNL 75

Query: 1040 K-KYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSD 1099
            +    G C ATNPIDRCWRC  NW++NRK L  CV GFGRKT GG  G  YVV D SD  
Sbjct: 76   RTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDS 135

Query: 1100 LVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITL 1159
            L +PK GTLR  VIQ  PLWIIF ++M IRL QELI+ +DKTID RGANVQIA GA +T+
Sbjct: 136  LTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTV 195

Query: 1160 QFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNC 1219
            QF++NVIIHG+ IH I  G GG IRDS  H G+RT SDGDGISI GSSNIWIDHVS++ C
Sbjct: 196  QFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARC 255

Query: 1220 HDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMP 1279
             DGLID I+GSTAITISNCH T H++VML GASD+Y+ D IMQVTVAFNHFG+GLVQRMP
Sbjct: 256  SDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMP 315

Query: 1280 RCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWK 1339
            RCR+GF HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IAP    AKEVTKR+Y+    W 
Sbjct: 316  RCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWS 375

Query: 1340 SWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRP 1399
             WTW+S+GDL ++GAFFV SG   +++ +S+ D+I  KPGTFV R+TRF+GAL C     
Sbjct: 376  KWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENME 434

BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.7e-152
Identity = 265/440 (60.23%), Postives = 313/440 (71.14%), Query Frame = 0

Query: 977  LSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGS---N 1036
            L++       ++ VFD YW  R  +A K    +++PHP  VT+  N  V   +  S   N
Sbjct: 20   LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 1037 STRRNL---------KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGK 1096
             TRR L          K +G C A NPID CWRCD NWA+NRKKLADCVLGFGR+T GGK
Sbjct: 80   DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 1097 LGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDAR 1156
             GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF R+M I+L QEL++TSDKTID R
Sbjct: 140  DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 1157 GANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFG 1216
            GA V I  GAG+TLQF+NNVIIH + + HIV G GG IRDS  H+GLRT SDGDGIS+FG
Sbjct: 200  GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 1217 SSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTV 1276
            ++NIWIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D +  D  MQ+TV
Sbjct: 260  ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 1277 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAK 1336
            AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  AK
Sbjct: 320  AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 1337 EVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMISYKPGT 1396
            ++TKREY+P   WKSW W+SEGD  +NGA+FV SG       +   P      I  KPGT
Sbjct: 380  QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 1397 FVTRMTRFAGALPCFVGRPC 1400
             V ++T  AG L C +G  C
Sbjct: 440  MVRKLTMDAGVLGCKLGEAC 459

BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 6.1e-149
Identity = 260/467 (55.67%), Postives = 323/467 (69.16%), Query Frame = 0

Query: 970  MVCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE----- 1029
            +VC++   SL   I        D+YW  +A EA+K    A+ P P  + D  +       
Sbjct: 9    LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 1030 -------------------VGQVLTGSNSTRRNL------------KKYTGPCRATNPID 1089
                               +  + + +NSTRR+L             K TGPC A+NPID
Sbjct: 69   TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 1090 RCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQ 1149
            +CWRC P+WA  RKKL  CV GFG +T GGK G  YVV    D D+VNP+PGTLRHAVIQ
Sbjct: 129  KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 1150 KGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHH 1209
            K PLWI+F  +M+IRL+QEL++TSDKTIDARGANV IAYGAGIT+Q+++N+IIHGL +HH
Sbjct: 189  KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 1210 IVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAIT 1269
            IV  +GG IRDS++H G R  +DGDGISIFG++NIW+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249  IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 1270 ISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 1329
            ISN HFTHHN+VML GA ++   D  MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309  ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 1330 HWLMYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNG 1389
            HW +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E+ WK+W WRSE D+ MN 
Sbjct: 369  HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 1390 AFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
            A+F  SG+       SR  MI  K G  V+++T++AGAL C VG+ C
Sbjct: 429  AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Sgr029085 vs. ExPASy TrEMBL
Match: A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)

HSP 1 Score: 2378.6 bits (6163), Expect = 0.0e+00
Identity = 1157/1419 (81.54%), Postives = 1228/1419 (86.54%), Query Frame = 0

Query: 8    MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
            MS+I LDPL YHC K + RFH RPRFN LK CSF+YCPNKLLCHGR++F +YNSYRLP  
Sbjct: 1    MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 68   RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
            +A++TNA    TF+S DVL KETFP+KR EK+EGRI V+LAQGK+  +WELTVGCNL GK
Sbjct: 61   KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 128  WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
            WILHWGVSLI DSGSEWDQPPKEMIPPGSI IKDYAIETPLKKSSSSS GD VHEVKIDL
Sbjct: 121  WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 188  VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
             P+K I+AINFVLKDEETG+W+QHKGRDFKVP  DY G+DGNIVG K+ LGLW       
Sbjct: 181  APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 248  GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
                       GALGQLSNLLVK E+ SK Q SS ESGDTKE+KK LEGFYEELPIVKEV
Sbjct: 241  -----------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEV 300

Query: 308  AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
            AVDNS+SVSV+K                           D+TRKWEIPAAPHPPETTVFK
Sbjct: 301  AVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFK 360

Query: 368  NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
            NKALRT LQP EG KGC G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP  SS NLP
Sbjct: 361  NKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLP 420

Query: 428  DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
            DQQR+S+L+++G S K+GE SEGVS  AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+E+
Sbjct: 421  DQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQEN 480

Query: 488  ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
            ILQEIEKLAAEAYSIFRS+APTFTE  +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481  ILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESH 540

Query: 548  KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
            KSGRWYM LKEKAAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541  KSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600

Query: 608  KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
            KTFHDVGI VLGD VLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601  KTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660

Query: 668  DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
            DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661  DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720

Query: 728  AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
            AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721  AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780

Query: 788  LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
            LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY+LTHPGTPSVF+
Sbjct: 781  LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFY 840

Query: 848  DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 907
            DHIFSHY SEIAAL+SLRKRNKVNCRSVVKI KAERDVYAATIDE VA+KIGPG+FEPPS
Sbjct: 841  DHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPS 900

Query: 908  GSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHY 967
            GSSGWSLV EG DY K EDNAAIGL IGDLYCSI   +  R    PIT  R         
Sbjct: 901  GSSGWSLVIEGKDY-KTEDNAAIGLCIGDLYCSICWLNPTRTFHQPITMAR--------- 960

Query: 968  TILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAVAIANADIGVFDDYWKARAI 1027
                                          + LLLTLSL    A ADIG FDDYWKARA 
Sbjct: 961  ------------------------------LSLLLTLSLLATTAFADIGDFDDYWKARAA 1020

Query: 1028 EAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1087
            EA++AAA A+EP+PE + DSLNAEVGQ L+GSNSTRRNLKKYTGPCRATNPIDRCWRCDP
Sbjct: 1021 EAEQAAADAYEPNPETIADSLNAEVGQALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1080

Query: 1088 NWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWII 1147
            NWA NRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDL+NPKPGTLRHAVIQKGPLWI 
Sbjct: 1081 NWAQNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIT 1140

Query: 1148 FDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGG 1207
            F  NMAIRLTQELI+TSDKTIDARGANVQIAYGAGITLQ++ NVIIHGLRIHHIVVG GG
Sbjct: 1141 FSTNMAIRLTQELIVTSDKTIDARGANVQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGG 1200

Query: 1208 TIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFT 1267
             IRD+VDHVGLRTMSDGDGISIFGSSN+WIDHVSMSNC DGLIDAIMGSTAITISNCHFT
Sbjct: 1201 MIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFT 1260

Query: 1268 HHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1327
            HHNEVMLFGASD YS D IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI
Sbjct: 1261 HHNEVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1320

Query: 1328 GGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGD 1387
            GGSQHPTI+SQGNRFIAPPNVYAKEVTKREYSPE VWKSWTWRSEGDLMMNGAFFVTSGD
Sbjct: 1321 GGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGD 1359

Query: 1388 QRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
            QRK RPFS  DMISYKPGT+V RMTRF+GAL CFVGRPC
Sbjct: 1381 QRKRRPFSSKDMISYKPGTYVKRMTRFSGALACFVGRPC 1359

BLAST of Sgr029085 vs. ExPASy TrEMBL
Match: A0A498HGM8 (Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_007136 PE=3 SV=1)

HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 906/1469 (61.67%), Postives = 1061/1469 (72.23%), Query Frame = 0

Query: 8    MSTIGLDPL---FYHCGKWEKRFHQRPRFN-TLK-TCSFSYCPNKLLCHGRRSFFNYNSY 67
            MST+ ++PL    +H G+ +K  H+RP+ N  LK + SF+  P KL+    RSF  +   
Sbjct: 13   MSTVRIEPLLQDLHHYGR-QKPSHRRPQSNHPLKLSSSFTAFPKKLVVSNSRSFCYFQP- 72

Query: 68   RLPAP--------RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQK 127
              P P        RA+ST+   +ET +S D + K+TFP+KRTE VEG+IFV+L  GKN+K
Sbjct: 73   --PTPRRGPTLGVRAASTDTTTVETSESADPIYKKTFPLKRTEVVEGKIFVKLDHGKNEK 132

Query: 128  QWELTVGCNLTGKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------- 187
            +W LTVGCNL GKW+LHWGVS + D   EW+QPP EM P GS+PIK              
Sbjct: 133  KWVLTVGCNLPGKWVLHWGVSFVDDVSCEWEQPPSEMRPAGSVPIKVCNWVLKFDCRAVG 192

Query: 188  -----------DYAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWF 247
                       DYAIETPLK+S SS  GDT +EVKID+ PN AI+AINFVLKDEETG W+
Sbjct: 193  WNEFDIHEDFQDYAIETPLKESLSSVGGDTSYEVKIDVKPNSAIAAINFVLKDEETGAWY 252

Query: 248  QHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLV 307
            QH+G DF+VP   Y  DD N+VG  +  G+W                  G LG+LSN+ V
Sbjct: 253  QHRGSDFRVPLVAYPQDDDNVVGATKGFGMWP-----------------GVLGKLSNVFV 312

Query: 308  KAEST-SKSQESSG-ESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK-------- 367
            KAES+ SK Q+SSG ES   +++   +EGFYEELPI KE++V+NSV+VSV K        
Sbjct: 313  KAESSNSKDQDSSGSESRHPQQKTTCVEGFYEELPIAKEISVNNSVTVSVSKCPETAKNL 372

Query: 368  -------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGI 427
                               D+T+ WEIPAAPHPPET VFK+KALRT LQ  EG  GC G+
Sbjct: 373  LYLETDLPDNAVVHWGVCRDDTKTWEIPAAPHPPETVVFKDKALRTRLQQKEGGNGCWGL 432

Query: 428  FTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEG 487
            FTLEE   GFLF+LK NE++WL   G+DFYIP SSS N    Q + + ++A V   + E 
Sbjct: 433  FTLEEGLAGFLFLLKLNESTWLRCVGNDFYIPLSSSKNANVVQSEIQSKDAQVPDGSTEA 492

Query: 488  SEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAA 547
             E     AY DG+I E+RNLV+D+ S ++ + R+K+A+E+ILQEIEKLAAEAYSIFR+  
Sbjct: 493  VE--ESTAYADGLINEMRNLVSDVFSDKSPRTRSKKAQEAILQEIEKLAAEAYSIFRTTV 552

Query: 548  PTFTEITL-ETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSL 607
            PT  E T+ ET +    P KI SGTGTGFEILCQGFNWES KSGRWYM LK KAA LSSL
Sbjct: 553  PTLPEETIAETEEVKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYMELKSKAALLSSL 612

Query: 608  GFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHR 667
            GFTVIW PPPTESVSPEGYMPRDLYNLNSRYGN+DELK+ VK FHDVGI VLGD VLNHR
Sbjct: 613  GFTVIWFPPPTESVSPEGYMPRDLYNLNSRYGNMDELKETVKVFHDVGIKVLGDAVLNHR 672

Query: 668  CAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRND 727
            CAH++NQNG+WNIFGGRLNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR D
Sbjct: 673  CAHYRNQNGVWNIFGGRLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKD 732

Query: 728  IKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHN 787
            I+EWLCWLR +IGYDGWRLDFVRGFWGGYV+DY+DASEPYFAVGEYWDSLSYTYGEMD N
Sbjct: 733  IREWLCWLRNDIGYDGWRLDFVRGFWGGYVRDYVDASEPYFAVGEYWDSLSYTYGEMDRN 792

Query: 788  QDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRA 847
            QD HRQRIVDWINATNGT GAFDVTTKGILH+AL+RCEYWRLSD+KG+PPGV+GWWPSRA
Sbjct: 793  QDAHRQRIVDWINATNGTCGAFDVTTKGILHAALERCEYWRLSDEKGKPPGVLGWWPSRA 852

Query: 848  VTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRK 907
            VTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR 
Sbjct: 853  VTFIENHDTGSTQGHWRFPKGKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRN 912

Query: 908  RNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAED 967
            RNK+NCRS+VKI KAERDVYAA IDEKVA+KIGPGH+EP SG   W+   EG DYK    
Sbjct: 913  RNKLNCRSLVKITKAERDVYAAIIDEKVAIKIGPGHYEPASGPQNWNKSLEGGDYK---- 972

Query: 968  NAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHP 1027
                 +W   L  S    S                                 G   +  P
Sbjct: 973  -----VWEASLVFSAPTRST------------------------------EDGAGGADRP 1032

Query: 1028 RKMAATTNRNMVCLL----LTLSLAVAIANADIGV--FDDYWKARAIEAQKAAATAFEPH 1087
                    ++  C              + N D  V   D YWK +A EA+K A  ++  +
Sbjct: 1033 ASAVQNRGQSAECGSGEHGFCNDNPTDVPNEDTLVLDLDAYWKEQAEEAEKVAMESYNKN 1092

Query: 1088 PEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASNRKKLA 1147
            PE VT+  N EVG++++  N+TRRNL   K YTGPC ATNP+D CWRCDPNWA+NRKK+ 
Sbjct: 1093 PEKVTEEFNTEVGKLMSKENTTRRNLRGNKAYTGPCMATNPMDACWRCDPNWANNRKKIV 1152

Query: 1148 DCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLT 1207
             C  GFG+KT GGK GP+YVV   +D D+ NP PGTLRHAVIQKGPLWIIF ++M IRL 
Sbjct: 1153 GCAQGFGKKTTGGKDGPFYVVTMGTDDDVQNPTPGTLRHAVIQKGPLWIIFTKSMVIRLQ 1212

Query: 1208 QELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVG 1267
            QEL++TSDKTIDARGANV I  GAGITLQF+ NVII  L I  IV   GG IRDSVDH+G
Sbjct: 1213 QELMVTSDKTIDARGANVVIEEGAGITLQFVKNVIITNLHIKMIVTKPGGLIRDSVDHIG 1272

Query: 1268 LRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGA 1327
            LRT SDGDGIS+FG+SN+WIDHVSMS C DGLIDAIMGSTAITISN HFT H+E MLFGA
Sbjct: 1273 LRTQSDGDGISLFGASNVWIDHVSMSRCADGLIDAIMGSTAITISNSHFTDHDEAMLFGA 1332

Query: 1328 SDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 1387
            +++++ D IMQ+T+AFNHFGQGLVQRMPR R GFFHVVNNDYTHW+MYAIGG+ +PTIIS
Sbjct: 1333 NNAHTQDKIMQITLAFNHFGQGLVQRMPRVRHGFFHVVNNDYTHWIMYAIGGNMNPTIIS 1392

Query: 1388 QGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL 1400
            QGNRFIAP N   K+VT RE +PE  WK+W WRSEGDLMMNGAFFV SG      P ++L
Sbjct: 1393 QGNRFIAPINGVTKQVTHRENTPEAEWKNWEWRSEGDLMMNGAFFVESGSGASKHP-AKL 1418

BLAST of Sgr029085 vs. ExPASy TrEMBL
Match: A0A498IC60 (Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_040744 PE=3 SV=1)

HSP 1 Score: 1711.4 bits (4431), Expect = 0.0e+00
Identity = 881/1468 (60.01%), Postives = 1039/1468 (70.78%), Query Frame = 0

Query: 8    MSTIGLDPLFYHCGKWEKRFHQRPRFNTLK-TCSFSYCPNKLLCHGRRSFFNYNSYRLPA 67
            MST+ ++PL +H  + +      P  + LK + SF+  P KL+    RSF N+    L +
Sbjct: 1    MSTVRIEPLLHHYRRQKPSHRLPPSKHPLKLSSSFTAFPKKLVVSNGRSFCNFQPPTL-S 60

Query: 68   PRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTG 127
             RA+ST+ A +E  +  D   KETFP+KRTE VEG++ V+L  GK+ K W LTVGCNL G
Sbjct: 61   VRAASTDTATVEATEFADAFYKETFPLKRTEVVEGKMIVKLDNGKDAKNWVLTVGCNLPG 120

Query: 128  KWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------------------D 187
            KW+LHWGV+ + D GSEWDQPP EM P GS+ IK                         D
Sbjct: 121  KWVLHWGVNYVDDVGSEWDQPPSEMRPAGSVSIKVRIWVLKFDCRAVGWHEFNKRVDFQD 180

Query: 188  YAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFS 247
            YAIETPLK+S S   GDT HEVKID+ PN AI+AINFVLKDEETG W+QH+GRDFKVPF 
Sbjct: 181  YAIETPLKESLSPVGGDTSHEVKIDVTPNSAIAAINFVLKDEETGAWYQHRGRDFKVPFV 240

Query: 248  DYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLVKAE-STSKSQES 307
             Y  DD N+VG  R+LG W                  G LG+LSN+ VKAE S SK QES
Sbjct: 241  CYLQDDDNVVGATRALGAWS-----------------GTLGKLSNVFVKAETSNSKDQES 300

Query: 308  SGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK--------------------- 367
            S ES D +++   LEGFYEELPI KE+AV++S +VSVRK                     
Sbjct: 301  SSESRDPQQKTMRLEGFYEELPIAKEIAVNHSATVSVRKCPETTKNLLYLETDLPDHAVV 360

Query: 368  ------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFV 427
                  D+ ++WEIPAAPHPPET VFK+KALRT LQ  E   GC G+FTLEE   GFLFV
Sbjct: 361  HWGVCRDDAKRWEIPAAPHPPETVVFKDKALRTRLQQREDGNGCSGLFTLEEGLAGFLFV 420

Query: 428  LKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGI 487
             K NE  WLN  G+DFYIP  SS N    Q + + E+A V  ++ E     +  AYTDGI
Sbjct: 421  FKLNETMWLNCVGNDFYIPLLSSNNSIAVQSEVQSEDAQVPDRSRE----TNFTAYTDGI 480

Query: 488  IKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAAPTF-TEITLETPK 547
            I EIRNLV+DI+S+++Q+KR+KEA+E+ILQEIEKLAAEAYSIFR+  PT   EI  ET K
Sbjct: 481  INEIRNLVSDISSEKSQRKRSKEAQETILQEIEKLAAEAYSIFRTTVPTLPEEIIAETEK 540

Query: 548  PVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTES 607
                P KI SGTGTGFEILCQGFNWES KSGRWY  LK KAAELSSLGFTVIW PPPT+S
Sbjct: 541  VKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYEELKSKAAELSSLGFTVIWFPPPTDS 600

Query: 608  VSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNI 667
            VSP+GYMPRDLYN+NSRYGN+DELK+ VKTFHD G+ VLGD VLNHRCA ++NQNG+WNI
Sbjct: 601  VSPQGYMPRDLYNMNSRYGNMDELKETVKTFHDAGLKVLGDAVLNHRCAEYQNQNGVWNI 660

Query: 668  FGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIG 727
            FGGRLNWD+RAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR DI+EWLCWLR +IG
Sbjct: 661  FGGRLNWDERAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKDIREWLCWLRDDIG 720

Query: 728  YDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWIN 787
            YDGWRLDFVRGFWGGYVKDY+DASEPYFAVGEYWDSLSYTYGEMDHNQD HRQRIVDWIN
Sbjct: 721  YDGWRLDFVRGFWGGYVKDYMDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWIN 780

Query: 788  ATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQ 847
            ATNGT GAFDVTTKGILH+AL+RCEYWRLSD KG+PPGV+GWWPSRAVTFIENHDTGSTQ
Sbjct: 781  ATNGTCGAFDVTTKGILHAALERCEYWRLSDTKGKPPGVLGWWPSRAVTFIENHDTGSTQ 840

Query: 848  GHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIE 907
            GHWRFP  KEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR RNK+NCRS VK++
Sbjct: 841  GHWRFPNKKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRNRNKLNCRSRVKLK 900

Query: 908  KAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYC 967
               +     T      MK  P   +    +    LV      K   +           + 
Sbjct: 901  SPRQ---KETSMRPSLMKRSPSKSDQVITN---LLVDLKIGIKALREETTRSGKRHKFFL 960

Query: 968  SISNPSVFRLRLN-------PITSPR-----FKAWPDNHYTILELSFLFSWGVKESLHPR 1027
                P++   R +       P ++ +      +     H +  ++S      V     P 
Sbjct: 961  HRHFPNLRDCRRSTEDETGRPASAAQNRGQSAECGSGEHVSSRKVS------VAADEVPT 1020

Query: 1028 KMAATTNRNMVCL-LLTLSLAV---------AIANADIGV--FDDYWKARAIEAQKAAAT 1087
             MAA  N +   L L  LS AV          +AN D  V   D YWK RA EA K A  
Sbjct: 1021 TMAAMFNSSYFFLCLFFLSFAVFVSSNDGPTDVANEDSTVLDLDAYWKERAQEAFKEAME 1080

Query: 1088 AFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASN 1147
            ++  +PE VT+  N  V ++L+  N+TRRNL   K Y GPC ATNPID CWRCDPNWA N
Sbjct: 1081 SYHKNPEQVTEEFNTGVAELLSKENTTRRNLRGNKGYNGPCMATNPIDACWRCDPNWADN 1140

Query: 1148 RKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNM 1207
            RKK+  C  GFG+K  GGK GP YVV   +D D+ NPKPGTLRHAVIQKGPLWIIF R+M
Sbjct: 1141 RKKIVGCAQGFGKKATGGKDGPIYVVTVGTDDDVQNPKPGTLRHAVIQKGPLWIIFARSM 1200

Query: 1208 AIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDS 1267
             IRL QEL++TSDKTID RGANV I  G GITLQF+ NVI+  L I  IV   GG IRDS
Sbjct: 1201 VIRLQQELMVTSDKTIDGRGANVVIEEGGGITLQFVKNVILTNLHIKMIVSKPGGMIRDS 1260

Query: 1268 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEV 1327
            VDH+GLRT SDGDGIS+FG+SN+WIDH+SMS C DGLIDA+M STAITISN HFT H++V
Sbjct: 1261 VDHMGLRTKSDGDGISLFGASNVWIDHMSMSRCADGLIDAVMASTAITISNSHFTDHDDV 1320

Query: 1328 MLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 1387
            MLFGA+++++ D IMQ+T+AFNHFG+GLVQRMPR R+GFFH+VNNDY HW+MYAIGG+ +
Sbjct: 1321 MLFGANNAHTQDKIMQITLAFNHFGRGLVQRMPRVRYGFFHLVNNDYNHWIMYAIGGNMN 1380

Query: 1388 PTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSR 1394
            PTIISQGNRFIAP N   K+VT RE++PE+ WK+W WRSEGDLMMNGAFFV SG +    
Sbjct: 1381 PTIISQGNRFIAPVNEATKQVTHREHTPESEWKNWEWRSEGDLMMNGAFFVESGSRASQN 1433

BLAST of Sgr029085 vs. ExPASy TrEMBL
Match: A0A4Y7JLT3 (Pectate lyase OS=Papaver somniferum OX=3469 GN=C5167_022499 PE=3 SV=1)

HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 810/1378 (58.78%), Postives = 960/1378 (69.67%), Query Frame = 0

Query: 82   SKDVLLKETFPIKRTEKVEGRIFVRLAQ---GKNQKQWELTVGCNLTGKWILHWGVSLIG 141
            +++VL+ +TFP+  T++VEG+I VRL +    + +++W LTVGCNL G W+LHWGVS   
Sbjct: 56   TENVLISKTFPLNWTQRVEGKISVRLDKKDGDEAEEKWVLTVGCNLPGNWVLHWGVSYTH 115

Query: 142  DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTV-HEVKIDLVPNKAISAIN 201
            D GSEWDQPP EM PPGSIPIKDYAIETPL +SS++  G     EV+I+     +ISAIN
Sbjct: 116  DIGSEWDQPPLEMRPPGSIPIKDYAIETPLMESSAALEGQLAQQEVQIEFDTPSSISAIN 175

Query: 202  FVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFN 261
            FV KDEETGVW QH+GRDFKVP +    +D N +G K+S+G+                  
Sbjct: 176  FVFKDEETGVWCQHRGRDFKVPLTGKALNDANSIGAKKSIGILP---------------- 235

Query: 262  IGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSV 321
                      ++K  S+   +E +GE  +  ++   LE FYEE  I+KE  V ++++VSV
Sbjct: 236  ----------VLKEGSSPNVEEKTGEVEEPAQQNMRLEAFYEEYSILKEFPVQSAITVSV 295

Query: 322  RK---------------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQP 381
             K                           D  +KWEIP APHP +TT FKNKAL+T LQ 
Sbjct: 296  TKSPDSEKNLVNFETNLPGDVVVHWGVCRDSDKKWEIPEAPHPAQTTTFKNKALQTILQL 355

Query: 382  IEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEE 441
             E  +G  G+F + EE  G LFVLK + N+WLN  G+DFYIP ++  ++  Q   +E   
Sbjct: 356  KEDGRGSSGVFPVGEELMGLLFVLKLSNNTWLNDMGNDFYIPLATETSIHTQTPAAE--- 415

Query: 442  AGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAA 501
                   GEGS        TD IIKEIR+LVTDI+S++  + ++KEA+E ILQEIEKLAA
Sbjct: 416  -----TGGEGS------VDTDEIIKEIRHLVTDISSEKRWQTKSKEAQEVILQEIEKLAA 475

Query: 502  EAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLK 561
            EAY IFRS  P F E   +     + P+KI+SGTG+GFEI+CQGFNWES+KSGRWY  L 
Sbjct: 476  EAYGIFRSTTPAFVEEVADAEVLEKTPIKITSGTGSGFEIVCQGFNWESNKSGRWYKELL 535

Query: 562  EKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITV 621
            +KA EL+S+GFTVIWLPPPT+SVSPEGYMPRDLYNLNSRYG+++ELK ++K FH+VGI  
Sbjct: 536  DKANELASIGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSMEELKVLLKKFHEVGIKT 595

Query: 622  LGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNID 681
            LGD VLNHRCA  +NQNG+WNIFGG+LNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNID
Sbjct: 596  LGDAVLNHRCAQKQNQNGVWNIFGGKLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNID 655

Query: 682  HSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLS 741
            HSQ+FVRND+KEWLCWLR EIGY GWRLDFVRGFWGGYVKDY++A+EPYF+VGEYWDSLS
Sbjct: 656  HSQEFVRNDLKEWLCWLRNEIGYHGWRLDFVRGFWGGYVKDYMEATEPYFSVGEYWDSLS 715

Query: 742  YTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPG 801
            Y+YGEMDHNQD HRQRI+DWINATNGT+GAFDVTTKGILH+ L+RCEYWRLSD KG+PPG
Sbjct: 716  YSYGEMDHNQDAHRQRIIDWINATNGTSGAFDVTTKGILHATLERCEYWRLSDSKGKPPG 775

Query: 802  VVGWWPSRAVTFIENHDTGSTQ-----------------------------GHWRFPGGK 861
            VVGWWPSRAVTFIENHDTGSTQ                                +FP GK
Sbjct: 776  VVGWWPSRAVTFIENHDTGSTQVGIAYRFIAEITSCSNLIATNTDIVPFPNTLEKFPEGK 835

Query: 862  EMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAA 921
            EMQGYAYILTHPGTPSVF+DHIFSH  SEIA L+S+R RNK+NCRS + I KAERDVYAA
Sbjct: 836  EMQGYAYILTHPGTPSVFYDHIFSH-KSEIAGLISIRNRNKINCRSTINITKAERDVYAA 895

Query: 922  TIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFR 981
             IDEKVAMKIGPGH+EPP+GS  W    EG DYK  +                       
Sbjct: 896  IIDEKVAMKIGPGHYEPPNGSE-WKSAFEGRDYKTLD----------------------- 955

Query: 982  LRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAV 1041
                                                  +K+++TT R  +          
Sbjct: 956  --------------------------------------KKVSSTTLREEL---------E 1015

Query: 1042 AIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKK 1101
                A I  FDD+W  R  EA++ A   + P+PE VT   N  V   L G+NSTRR LKK
Sbjct: 1016 KQFQAHIRDFDDHWLQRQDEARRFAMEVYHPNPENVTAHFNEHVRIALEGTNSTRRTLKK 1075

Query: 1102 YTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVN 1161
            +TG C ATNPIDRCWRC PNWA++RK+LA CV GFGR T+GGK G  YVV + SD+DL N
Sbjct: 1076 HTGRCLATNPIDRCWRCRPNWATDRKRLASCVKGFGRNTVGGKFGRIYVVTNPSDNDLAN 1135

Query: 1162 PKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFI 1221
            P+PGTLRHA IQK PLWIIF R+M I L QEL++ S KTID RGANV IAYGAGIT+QF 
Sbjct: 1136 PRPGTLRHAAIQKEPLWIIFARSMLITLQQELMVQSHKTIDGRGANVHIAYGAGITVQFA 1195

Query: 1222 NNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDG 1281
             NVIIH L IH IV   GG IRDSVDH+G RT SDGD +SI+GSSN+W+DH+SMSNC DG
Sbjct: 1196 RNVIIHNLHIHDIVSRNGGLIRDSVDHIGFRTASDGDAVSIYGSSNVWVDHLSMSNCKDG 1255

Query: 1282 LIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCR 1341
            L+D I GST IT+SN H THHNEVMLFGA D    D IMQVTVAFNH+GQGL+QRMPRCR
Sbjct: 1256 LVDIIEGSTGITVSNNHMTHHNEVMLFGAHDDQPLDQIMQVTVAFNHYGQGLIQRMPRCR 1315

Query: 1342 WGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWT 1400
            WGF HVVNNDYTHWLMYAIGGSQHPTI+SQGNRFIAPP   AKEVT R  +P++VWK WT
Sbjct: 1316 WGFIHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPTQAAKEVTHRSDAPQSVWKDWT 1321

BLAST of Sgr029085 vs. ExPASy TrEMBL
Match: A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)

HSP 1 Score: 1595.1 bits (4129), Expect = 0.0e+00
Identity = 783/915 (85.57%), Postives = 818/915 (89.40%), Query Frame = 0

Query: 8   MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
           MSTIGLDPL YHCGK + RFH+RP+FNTLK CSF Y PNKLL  GRRSFFN NSYRLPA 
Sbjct: 1   MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60

Query: 68  RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
           R SSTNAALLE+F+S DVLLKETFP+KRTEKVEG IFVRLAQGKNQ QWELTVGCNLTGK
Sbjct: 61  RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120

Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
           WILHWGVSLI DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180

Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
           VPNK I+AINFVLKDEETG+W+QHKGRDFKVP +D HGDDGNIVGTK+ LGLW       
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWP------ 240

Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
                      GALGQLSNLLVKAES SK QESSGESGDTKE  KYLEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKAES-SKGQESSGESGDTKE--KYLEGFYEELPIVKEV 300

Query: 308 AVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTVFK 367
           A DNSVSVSVR                           +DETRKWEIPAAPHPPETTVFK
Sbjct: 301 AFDNSVSVSVRNCPETTKYLLYLETDLPGDVIVHWGASRDETRKWEIPAAPHPPETTVFK 360

Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
           NKALRT LQ  EG KG CGIFT+EEEF+GFLFVLKQNEN WLNYKGDDFYIPF SS +LP
Sbjct: 361 NKALRTLLQLKEGGKGSCGIFTIEEEFDGFLFVLKQNENPWLNYKGDDFYIPFPSSGHLP 420

Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
            Q+RQ+E E+AGVS KAG+G E VS  AYTDGIIKEIRN VTDI+SQRTQKK+T EA+ES
Sbjct: 421 GQKRQNEFEDAGVSRKAGDGYEEVSVTAYTDGIIKEIRNFVTDISSQRTQKKKTIEAQES 480

Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
           ILQEIEKLAAEAYSIFRSA+PTFTE  +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSASPTFTEEIIEMPKPVEPPVQISSGTGSGFEILCQGFNWESH 540

Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
           KSGRWYM LKEKAAELSSLGFTVIWLPPPTESVSPEGYMP+DLYNL+SRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKEKAAELSSLGFTVIWLPPPTESVSPEGYMPKDLYNLSSRYGNIDELKDVV 600

Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
           KTFHDVGI VLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660

Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
           DNFHAAPNIDHSQDFVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLRWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720

Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
           AVGEYWDSLSYTYGEMDH+QD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHDQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780

Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
           LSDQKG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY LTHPGTPSVFF
Sbjct: 781 LSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYTLTHPGTPSVFF 840

Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 896
           DHIFSHY S+IAAL+SLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPG++EPPS
Sbjct: 841 DHIFSHYKSDIAALISLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGYYEPPS 895

BLAST of Sgr029085 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/857 (66.28%), Postives = 666/857 (77.71%), Query Frame = 0

Query: 66  APRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLT 125
           A RA+S++ A++ET +S DV+ KE FP++R EK EG+I+VRL + K +K WEL+VGC++ 
Sbjct: 63  AIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVK-EKNWELSVGCSIP 122

Query: 126 GKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKI 185
           GKWILHWGVS +GD+GSEWDQPP++M PPGSI IKDYAIETPLKK S    GD+  EV I
Sbjct: 123 GKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEVAI 182

Query: 186 DLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVY 245
           +L    +++A+NFVLKDEETG W+QHKGRDFKVP  D   D+GN++G K+          
Sbjct: 183 NLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKK---------- 242

Query: 246 HVGGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVK 305
                        GALGQLSN+ +K       Q+ S    D+ EE+K L+ FYEE+PI K
Sbjct: 243 -----------GFGALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISK 302

Query: 306 EVAVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTV 365
            VA DNSVSV+ R                           K+ T+KWEIP+ P+P ET++
Sbjct: 303 RVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSL 362

Query: 366 FKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVN 425
           FKNKALRT LQ  +   G  G+F+L+ + EG  FVLK NEN+WLNY+G+DFY+PF +S +
Sbjct: 363 FKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSS 422

Query: 426 LPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAK 485
            P      E E A VS    +  + VS   +T  II EIRNL  DI+S + QK   KE +
Sbjct: 423 SP-----VETEAAQVSKPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQ 482

Query: 486 ESILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWE 545
           E+ILQEIEKLAAEAYSIFRS  P F+E  +   +  +P +KISSGTG+GFEILCQGFNWE
Sbjct: 483 ENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWE 542

Query: 546 SHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKD 605
           S+KSGRWY+ L+EKA EL+SLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYG IDELKD
Sbjct: 543 SNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKD 602

Query: 606 VVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKS 665
            VK FH VGI VLGD VLNHRCAHFKNQNG+WN+FGGRLNWDDRAVV+DDPHFQGRGNKS
Sbjct: 603 TVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKS 662

Query: 666 SGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEP 725
           SGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+P
Sbjct: 663 SGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKP 722

Query: 726 YFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEY 785
           YFAVGEYWDSLSYTYGEMD+NQD HRQRIVDWINAT+G AGAFDVTTKGILH+AL +CEY
Sbjct: 723 YFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEY 782

Query: 786 WRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSV 845
           WRLSD KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+V
Sbjct: 783 WRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAV 842

Query: 846 FFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEP 896
           FFDHIFS Y+SEIAAL+SLR R K++CRS V I+K+ERDVYAA IDEKVAMKIGPGH+EP
Sbjct: 843 FFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEP 882

BLAST of Sgr029085 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 541.6 bits (1394), Expect = 1.9e-153
Identity = 265/440 (60.23%), Postives = 313/440 (71.14%), Query Frame = 0

Query: 977  LSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGS---N 1036
            L++       ++ VFD YW  R  +A K    +++PHP  VT+  N  V   +  S   N
Sbjct: 20   LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 1037 STRRNL---------KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGK 1096
             TRR L          K +G C A NPID CWRCD NWA+NRKKLADCVLGFGR+T GGK
Sbjct: 80   DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 1097 LGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDAR 1156
             GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF R+M I+L QEL++TSDKTID R
Sbjct: 140  DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 1157 GANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFG 1216
            GA V I  GAG+TLQF+NNVIIH + + HIV G GG IRDS  H+GLRT SDGDGIS+FG
Sbjct: 200  GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 1217 SSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTV 1276
            ++NIWIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D +  D  MQ+TV
Sbjct: 260  ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 1277 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAK 1336
            AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  AK
Sbjct: 320  AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 1337 EVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMISYKPGT 1396
            ++TKREY+P   WKSW W+SEGD  +NGA+FV SG       +   P      I  KPGT
Sbjct: 380  QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 1397 FVTRMTRFAGALPCFVGRPC 1400
             V ++T  AG L C +G  C
Sbjct: 440  MVRKLTMDAGVLGCKLGEAC 459

BLAST of Sgr029085 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 4.4e-150
Identity = 260/467 (55.67%), Postives = 323/467 (69.16%), Query Frame = 0

Query: 970  MVCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE----- 1029
            +VC++   SL   I        D+YW  +A EA+K    A+ P P  + D  +       
Sbjct: 9    LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 1030 -------------------VGQVLTGSNSTRRNL------------KKYTGPCRATNPID 1089
                               +  + + +NSTRR+L             K TGPC A+NPID
Sbjct: 69   TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 1090 RCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQ 1149
            +CWRC P+WA  RKKL  CV GFG +T GGK G  YVV    D D+VNP+PGTLRHAVIQ
Sbjct: 129  KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 1150 KGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHH 1209
            K PLWI+F  +M+IRL+QEL++TSDKTIDARGANV IAYGAGIT+Q+++N+IIHGL +HH
Sbjct: 189  KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 1210 IVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAIT 1269
            IV  +GG IRDS++H G R  +DGDGISIFG++NIW+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249  IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 1270 ISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 1329
            ISN HFTHHN+VML GA ++   D  MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309  ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 1330 HWLMYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNG 1389
            HW +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E+ WK+W WRSE D+ MN 
Sbjct: 369  HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 1390 AFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
            A+F  SG+       SR  MI  K G  V+++T++AGAL C VG+ C
Sbjct: 429  AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Sgr029085 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 2.4e-148
Identity = 258/464 (55.60%), Postives = 320/464 (68.97%), Query Frame = 0

Query: 973  LLLTLSLAVAI--ANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE------ 1032
            L+ T  +AV I     +I   D+YW  RA EA++    A+   P  + D  +        
Sbjct: 9    LMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNST 68

Query: 1033 --------------------VGQVLTGSNSTRRNLK--------KYTGPCRATNPIDRCW 1092
                                +  + + +NSTRR+L+        K  GPC A+NPID+CW
Sbjct: 69   DVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCW 128

Query: 1093 RCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGP 1152
            RC  +WA  RKKL  CV GFG +T GGK G  YVV  + D D+VNPKPGTLRHAVIQK P
Sbjct: 129  RCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEP 188

Query: 1153 LWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVV 1212
            LWIIF  +M+IRL QEL++ S KTIDARGANV +A+GAGIT+QF+ NV+IHGL IHHI  
Sbjct: 189  LWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISE 248

Query: 1213 GTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISN 1272
             +GG IRDSVDH G+RT +DGDG+SI+GSSNIW+DH+SMS C DGLIDAI+GST ITISN
Sbjct: 249  SSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISN 308

Query: 1273 CHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 1332
             HFTHHN+VML GA ++   D  MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 309  SHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWE 368

Query: 1333 MYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFF 1392
            +YAIGGSQ PTI+S GNRFIAPP+  + +EVTKR+Y+ E+ WK W WRS+ D+ MNGA+F
Sbjct: 369  LYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYF 428

Query: 1393 VTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
              SG+ +     +R  MI  K G  V+++T++AGAL C VGR C
Sbjct: 429  RQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Sgr029085 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 499.6 bits (1285), Expect = 8.2e-141
Identity = 257/446 (57.62%), Postives = 310/446 (69.51%), Query Frame = 0

Query: 971  VCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLN--AEVGQV 1030
            V + L+L++ V    A I  +D+YW  R   A +    +++P+PE VTD  N  A +   
Sbjct: 10   VFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAME 69

Query: 1031 LTG-SNSTRRNL------KKYT---GPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGR 1090
             TG  N TRR+L      KK T   G   + N ID+CWR D NW  NRKKLADCVLGFGR
Sbjct: 70   TTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGR 129

Query: 1091 KTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSD 1150
            KT GGK GP YVV D SD+DL+ PKPGT+RHAV +  PLWIIF R+M I+L QELI+T+D
Sbjct: 130  KTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITND 189

Query: 1151 KTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGD 1210
            KTID RGA + I  GAG+TLQF+ NVIIH + I  I  G GG I DS  H GLRT+SDGD
Sbjct: 190  KTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGD 249

Query: 1211 GISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDA 1270
            GI+IFG++N+WIDHVSM++C DG+IDAIMGSTAITISN HFT H+EVMLFG ++    D 
Sbjct: 250  GINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDK 309

Query: 1271 IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 1330
             MQ+TVAFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAP
Sbjct: 310  KMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAP 369

Query: 1331 PNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMI 1390
            P   +K+VTKREY+P   WKSW W+SE D  +NGA+FV SG           P  R   I
Sbjct: 370  PIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAI 429

Query: 1391 SYKPGTFVTRMTRFAGALPCFVGRPC 1400
              +PGT V R+T+ AG L C  G+ C
Sbjct: 430  RPQPGTKVRRLTKDAGTLGCKPGKSC 455