Homology
BLAST of Sgr029085 vs. NCBI nr
Match:
TYK15529.1 (alpha-amylase 3 [Cucumis melo var. makuwa])
HSP 1 Score: 2378.6 bits (6163), Expect = 0.0e+00
Identity = 1157/1419 (81.54%), Postives = 1228/1419 (86.54%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
MS+I LDPL YHC K + RFH RPRFN LK CSF+YCPNKLLCHGR++F +YNSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 68 RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
+A++TNA TF+S DVL KETFP+KR EK+EGRI V+LAQGK+ +WELTVGCNL GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
WILHWGVSLI DSGSEWDQPPKEMIPPGSI IKDYAIETPLKKSSSSS GD VHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
P+K I+AINFVLKDEETG+W+QHKGRDFKVP DY G+DGNIVG K+ LGLW
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
GALGQLSNLLVK E+ SK Q SS ESGDTKE+KK LEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEV 300
Query: 308 AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
AVDNS+SVSV+K D+TRKWEIPAAPHPPETTVFK
Sbjct: 301 AVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFK 360
Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
NKALRT LQP EG KGC G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP SS NLP
Sbjct: 361 NKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLP 420
Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
DQQR+S+L+++G S K+GE SEGVS AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+E+
Sbjct: 421 DQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQEN 480
Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
ILQEIEKLAAEAYSIFRS+APTFTE +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESH 540
Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
KSGRWYM LKEKAAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600
Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
KTFHDVGI VLGD VLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660
Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720
Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780
Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY+LTHPGTPSVF+
Sbjct: 781 LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFY 840
Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 907
DHIFSHY SEIAAL+SLRKRNKVNCRSVVKI KAERDVYAATIDE VA+KIGPG+FEPPS
Sbjct: 841 DHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPS 900
Query: 908 GSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHY 967
GSSGWSLV EG DY K EDNAAIGL IGDLYCSI + R PIT R
Sbjct: 901 GSSGWSLVIEGKDY-KTEDNAAIGLCIGDLYCSICWLNPTRTFHQPITMAR--------- 960
Query: 968 TILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAVAIANADIGVFDDYWKARAI 1027
+ LLLTLSL A ADIG FDDYWKARA
Sbjct: 961 ------------------------------LSLLLTLSLLATTAFADIGDFDDYWKARAA 1020
Query: 1028 EAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1087
EA++AAA A+EP+PE + DSLNAEVGQ L+GSNSTRRNLKKYTGPCRATNPIDRCWRCDP
Sbjct: 1021 EAEQAAADAYEPNPETIADSLNAEVGQALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1080
Query: 1088 NWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWII 1147
NWA NRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDL+NPKPGTLRHAVIQKGPLWI
Sbjct: 1081 NWAQNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIT 1140
Query: 1148 FDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGG 1207
F NMAIRLTQELI+TSDKTIDARGANVQIAYGAGITLQ++ NVIIHGLRIHHIVVG GG
Sbjct: 1141 FSTNMAIRLTQELIVTSDKTIDARGANVQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGG 1200
Query: 1208 TIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFT 1267
IRD+VDHVGLRTMSDGDGISIFGSSN+WIDHVSMSNC DGLIDAIMGSTAITISNCHFT
Sbjct: 1201 MIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFT 1260
Query: 1268 HHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1327
HHNEVMLFGASD YS D IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI
Sbjct: 1261 HHNEVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1320
Query: 1328 GGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGD 1387
GGSQHPTI+SQGNRFIAPPNVYAKEVTKREYSPE VWKSWTWRSEGDLMMNGAFFVTSGD
Sbjct: 1321 GGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGD 1359
Query: 1388 QRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
QRK RPFS DMISYKPGT+V RMTRF+GAL CFVGRPC
Sbjct: 1381 QRKRRPFSSKDMISYKPGTYVKRMTRFSGALACFVGRPC 1359
BLAST of Sgr029085 vs. NCBI nr
Match:
RXH69880.1 (hypothetical protein DVH24_007136 [Malus domestica])
HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 906/1469 (61.67%), Postives = 1061/1469 (72.23%), Query Frame = 0
Query: 8 MSTIGLDPL---FYHCGKWEKRFHQRPRFN-TLK-TCSFSYCPNKLLCHGRRSFFNYNSY 67
MST+ ++PL +H G+ +K H+RP+ N LK + SF+ P KL+ RSF +
Sbjct: 13 MSTVRIEPLLQDLHHYGR-QKPSHRRPQSNHPLKLSSSFTAFPKKLVVSNSRSFCYFQP- 72
Query: 68 RLPAP--------RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQK 127
P P RA+ST+ +ET +S D + K+TFP+KRTE VEG+IFV+L GKN+K
Sbjct: 73 --PTPRRGPTLGVRAASTDTTTVETSESADPIYKKTFPLKRTEVVEGKIFVKLDHGKNEK 132
Query: 128 QWELTVGCNLTGKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------- 187
+W LTVGCNL GKW+LHWGVS + D EW+QPP EM P GS+PIK
Sbjct: 133 KWVLTVGCNLPGKWVLHWGVSFVDDVSCEWEQPPSEMRPAGSVPIKVCNWVLKFDCRAVG 192
Query: 188 -----------DYAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWF 247
DYAIETPLK+S SS GDT +EVKID+ PN AI+AINFVLKDEETG W+
Sbjct: 193 WNEFDIHEDFQDYAIETPLKESLSSVGGDTSYEVKIDVKPNSAIAAINFVLKDEETGAWY 252
Query: 248 QHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLV 307
QH+G DF+VP Y DD N+VG + G+W G LG+LSN+ V
Sbjct: 253 QHRGSDFRVPLVAYPQDDDNVVGATKGFGMWP-----------------GVLGKLSNVFV 312
Query: 308 KAEST-SKSQESSG-ESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK-------- 367
KAES+ SK Q+SSG ES +++ +EGFYEELPI KE++V+NSV+VSV K
Sbjct: 313 KAESSNSKDQDSSGSESRHPQQKTTCVEGFYEELPIAKEISVNNSVTVSVSKCPETAKNL 372
Query: 368 -------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGI 427
D+T+ WEIPAAPHPPET VFK+KALRT LQ EG GC G+
Sbjct: 373 LYLETDLPDNAVVHWGVCRDDTKTWEIPAAPHPPETVVFKDKALRTRLQQKEGGNGCWGL 432
Query: 428 FTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEG 487
FTLEE GFLF+LK NE++WL G+DFYIP SSS N Q + + ++A V + E
Sbjct: 433 FTLEEGLAGFLFLLKLNESTWLRCVGNDFYIPLSSSKNANVVQSEIQSKDAQVPDGSTEA 492
Query: 488 SEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAA 547
E AY DG+I E+RNLV+D+ S ++ + R+K+A+E+ILQEIEKLAAEAYSIFR+
Sbjct: 493 VE--ESTAYADGLINEMRNLVSDVFSDKSPRTRSKKAQEAILQEIEKLAAEAYSIFRTTV 552
Query: 548 PTFTEITL-ETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSL 607
PT E T+ ET + P KI SGTGTGFEILCQGFNWES KSGRWYM LK KAA LSSL
Sbjct: 553 PTLPEETIAETEEVKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYMELKSKAALLSSL 612
Query: 608 GFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHR 667
GFTVIW PPPTESVSPEGYMPRDLYNLNSRYGN+DELK+ VK FHDVGI VLGD VLNHR
Sbjct: 613 GFTVIWFPPPTESVSPEGYMPRDLYNLNSRYGNMDELKETVKVFHDVGIKVLGDAVLNHR 672
Query: 668 CAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRND 727
CAH++NQNG+WNIFGGRLNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR D
Sbjct: 673 CAHYRNQNGVWNIFGGRLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKD 732
Query: 728 IKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHN 787
I+EWLCWLR +IGYDGWRLDFVRGFWGGYV+DY+DASEPYFAVGEYWDSLSYTYGEMD N
Sbjct: 733 IREWLCWLRNDIGYDGWRLDFVRGFWGGYVRDYVDASEPYFAVGEYWDSLSYTYGEMDRN 792
Query: 788 QDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRA 847
QD HRQRIVDWINATNGT GAFDVTTKGILH+AL+RCEYWRLSD+KG+PPGV+GWWPSRA
Sbjct: 793 QDAHRQRIVDWINATNGTCGAFDVTTKGILHAALERCEYWRLSDEKGKPPGVLGWWPSRA 852
Query: 848 VTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRK 907
VTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR
Sbjct: 853 VTFIENHDTGSTQGHWRFPKGKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRN 912
Query: 908 RNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAED 967
RNK+NCRS+VKI KAERDVYAA IDEKVA+KIGPGH+EP SG W+ EG DYK
Sbjct: 913 RNKLNCRSLVKITKAERDVYAAIIDEKVAIKIGPGHYEPASGPQNWNKSLEGGDYK---- 972
Query: 968 NAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHP 1027
+W L S S G + P
Sbjct: 973 -----VWEASLVFSAPTRST------------------------------EDGAGGADRP 1032
Query: 1028 RKMAATTNRNMVCLL----LTLSLAVAIANADIGV--FDDYWKARAIEAQKAAATAFEPH 1087
++ C + N D V D YWK +A EA+K A ++ +
Sbjct: 1033 ASAVQNRGQSAECGSGEHGFCNDNPTDVPNEDTLVLDLDAYWKEQAEEAEKVAMESYNKN 1092
Query: 1088 PEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASNRKKLA 1147
PE VT+ N EVG++++ N+TRRNL K YTGPC ATNP+D CWRCDPNWA+NRKK+
Sbjct: 1093 PEKVTEEFNTEVGKLMSKENTTRRNLRGNKAYTGPCMATNPMDACWRCDPNWANNRKKIV 1152
Query: 1148 DCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLT 1207
C GFG+KT GGK GP+YVV +D D+ NP PGTLRHAVIQKGPLWIIF ++M IRL
Sbjct: 1153 GCAQGFGKKTTGGKDGPFYVVTMGTDDDVQNPTPGTLRHAVIQKGPLWIIFTKSMVIRLQ 1212
Query: 1208 QELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVG 1267
QEL++TSDKTIDARGANV I GAGITLQF+ NVII L I IV GG IRDSVDH+G
Sbjct: 1213 QELMVTSDKTIDARGANVVIEEGAGITLQFVKNVIITNLHIKMIVTKPGGLIRDSVDHIG 1272
Query: 1268 LRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGA 1327
LRT SDGDGIS+FG+SN+WIDHVSMS C DGLIDAIMGSTAITISN HFT H+E MLFGA
Sbjct: 1273 LRTQSDGDGISLFGASNVWIDHVSMSRCADGLIDAIMGSTAITISNSHFTDHDEAMLFGA 1332
Query: 1328 SDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 1387
+++++ D IMQ+T+AFNHFGQGLVQRMPR R GFFHVVNNDYTHW+MYAIGG+ +PTIIS
Sbjct: 1333 NNAHTQDKIMQITLAFNHFGQGLVQRMPRVRHGFFHVVNNDYTHWIMYAIGGNMNPTIIS 1392
Query: 1388 QGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL 1400
QGNRFIAP N K+VT RE +PE WK+W WRSEGDLMMNGAFFV SG P ++L
Sbjct: 1393 QGNRFIAPINGVTKQVTHRENTPEAEWKNWEWRSEGDLMMNGAFFVESGSGASKHP-AKL 1418
BLAST of Sgr029085 vs. NCBI nr
Match:
RXH79597.1 (hypothetical protein DVH24_040744 [Malus domestica])
HSP 1 Score: 1711.4 bits (4431), Expect = 0.0e+00
Identity = 881/1468 (60.01%), Postives = 1039/1468 (70.78%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLK-TCSFSYCPNKLLCHGRRSFFNYNSYRLPA 67
MST+ ++PL +H + + P + LK + SF+ P KL+ RSF N+ L +
Sbjct: 1 MSTVRIEPLLHHYRRQKPSHRLPPSKHPLKLSSSFTAFPKKLVVSNGRSFCNFQPPTL-S 60
Query: 68 PRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTG 127
RA+ST+ A +E + D KETFP+KRTE VEG++ V+L GK+ K W LTVGCNL G
Sbjct: 61 VRAASTDTATVEATEFADAFYKETFPLKRTEVVEGKMIVKLDNGKDAKNWVLTVGCNLPG 120
Query: 128 KWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------------------D 187
KW+LHWGV+ + D GSEWDQPP EM P GS+ IK D
Sbjct: 121 KWVLHWGVNYVDDVGSEWDQPPSEMRPAGSVSIKVRIWVLKFDCRAVGWHEFNKRVDFQD 180
Query: 188 YAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFS 247
YAIETPLK+S S GDT HEVKID+ PN AI+AINFVLKDEETG W+QH+GRDFKVPF
Sbjct: 181 YAIETPLKESLSPVGGDTSHEVKIDVTPNSAIAAINFVLKDEETGAWYQHRGRDFKVPFV 240
Query: 248 DYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLVKAE-STSKSQES 307
Y DD N+VG R+LG W G LG+LSN+ VKAE S SK QES
Sbjct: 241 CYLQDDDNVVGATRALGAWS-----------------GTLGKLSNVFVKAETSNSKDQES 300
Query: 308 SGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK--------------------- 367
S ES D +++ LEGFYEELPI KE+AV++S +VSVRK
Sbjct: 301 SSESRDPQQKTMRLEGFYEELPIAKEIAVNHSATVSVRKCPETTKNLLYLETDLPDHAVV 360
Query: 368 ------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFV 427
D+ ++WEIPAAPHPPET VFK+KALRT LQ E GC G+FTLEE GFLFV
Sbjct: 361 HWGVCRDDAKRWEIPAAPHPPETVVFKDKALRTRLQQREDGNGCSGLFTLEEGLAGFLFV 420
Query: 428 LKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGI 487
K NE WLN G+DFYIP SS N Q + + E+A V ++ E + AYTDGI
Sbjct: 421 FKLNETMWLNCVGNDFYIPLLSSNNSIAVQSEVQSEDAQVPDRSRE----TNFTAYTDGI 480
Query: 488 IKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAAPTF-TEITLETPK 547
I EIRNLV+DI+S+++Q+KR+KEA+E+ILQEIEKLAAEAYSIFR+ PT EI ET K
Sbjct: 481 INEIRNLVSDISSEKSQRKRSKEAQETILQEIEKLAAEAYSIFRTTVPTLPEEIIAETEK 540
Query: 548 PVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTES 607
P KI SGTGTGFEILCQGFNWES KSGRWY LK KAAELSSLGFTVIW PPPT+S
Sbjct: 541 VKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYEELKSKAAELSSLGFTVIWFPPPTDS 600
Query: 608 VSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNI 667
VSP+GYMPRDLYN+NSRYGN+DELK+ VKTFHD G+ VLGD VLNHRCA ++NQNG+WNI
Sbjct: 601 VSPQGYMPRDLYNMNSRYGNMDELKETVKTFHDAGLKVLGDAVLNHRCAEYQNQNGVWNI 660
Query: 668 FGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIG 727
FGGRLNWD+RAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR DI+EWLCWLR +IG
Sbjct: 661 FGGRLNWDERAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKDIREWLCWLRDDIG 720
Query: 728 YDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWIN 787
YDGWRLDFVRGFWGGYVKDY+DASEPYFAVGEYWDSLSYTYGEMDHNQD HRQRIVDWIN
Sbjct: 721 YDGWRLDFVRGFWGGYVKDYMDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWIN 780
Query: 788 ATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQ 847
ATNGT GAFDVTTKGILH+AL+RCEYWRLSD KG+PPGV+GWWPSRAVTFIENHDTGSTQ
Sbjct: 781 ATNGTCGAFDVTTKGILHAALERCEYWRLSDTKGKPPGVLGWWPSRAVTFIENHDTGSTQ 840
Query: 848 GHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIE 907
GHWRFP KEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR RNK+NCRS VK++
Sbjct: 841 GHWRFPNKKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRNRNKLNCRSRVKLK 900
Query: 908 KAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYC 967
+ T MK P + + LV K + +
Sbjct: 901 SPRQ---KETSMRPSLMKRSPSKSDQVITN---LLVDLKIGIKALREETTRSGKRHKFFL 960
Query: 968 SISNPSVFRLRLN-------PITSPR-----FKAWPDNHYTILELSFLFSWGVKESLHPR 1027
P++ R + P ++ + + H + ++S V P
Sbjct: 961 HRHFPNLRDCRRSTEDETGRPASAAQNRGQSAECGSGEHVSSRKVS------VAADEVPT 1020
Query: 1028 KMAATTNRNMVCL-LLTLSLAV---------AIANADIGV--FDDYWKARAIEAQKAAAT 1087
MAA N + L L LS AV +AN D V D YWK RA EA K A
Sbjct: 1021 TMAAMFNSSYFFLCLFFLSFAVFVSSNDGPTDVANEDSTVLDLDAYWKERAQEAFKEAME 1080
Query: 1088 AFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASN 1147
++ +PE VT+ N V ++L+ N+TRRNL K Y GPC ATNPID CWRCDPNWA N
Sbjct: 1081 SYHKNPEQVTEEFNTGVAELLSKENTTRRNLRGNKGYNGPCMATNPIDACWRCDPNWADN 1140
Query: 1148 RKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNM 1207
RKK+ C GFG+K GGK GP YVV +D D+ NPKPGTLRHAVIQKGPLWIIF R+M
Sbjct: 1141 RKKIVGCAQGFGKKATGGKDGPIYVVTVGTDDDVQNPKPGTLRHAVIQKGPLWIIFARSM 1200
Query: 1208 AIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDS 1267
IRL QEL++TSDKTID RGANV I G GITLQF+ NVI+ L I IV GG IRDS
Sbjct: 1201 VIRLQQELMVTSDKTIDGRGANVVIEEGGGITLQFVKNVILTNLHIKMIVSKPGGMIRDS 1260
Query: 1268 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEV 1327
VDH+GLRT SDGDGIS+FG+SN+WIDH+SMS C DGLIDA+M STAITISN HFT H++V
Sbjct: 1261 VDHMGLRTKSDGDGISLFGASNVWIDHMSMSRCADGLIDAVMASTAITISNSHFTDHDDV 1320
Query: 1328 MLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 1387
MLFGA+++++ D IMQ+T+AFNHFG+GLVQRMPR R+GFFH+VNNDY HW+MYAIGG+ +
Sbjct: 1321 MLFGANNAHTQDKIMQITLAFNHFGRGLVQRMPRVRYGFFHLVNNDYNHWIMYAIGGNMN 1380
Query: 1388 PTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSR 1394
PTIISQGNRFIAP N K+VT RE++PE+ WK+W WRSEGDLMMNGAFFV SG +
Sbjct: 1381 PTIISQGNRFIAPVNEATKQVTHREHTPESEWKNWEWRSEGDLMMNGAFFVESGSRASQN 1433
BLAST of Sgr029085 vs. NCBI nr
Match:
RZC60748.1 (hypothetical protein C5167_022499 [Papaver somniferum])
HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 810/1378 (58.78%), Postives = 960/1378 (69.67%), Query Frame = 0
Query: 82 SKDVLLKETFPIKRTEKVEGRIFVRLAQ---GKNQKQWELTVGCNLTGKWILHWGVSLIG 141
+++VL+ +TFP+ T++VEG+I VRL + + +++W LTVGCNL G W+LHWGVS
Sbjct: 56 TENVLISKTFPLNWTQRVEGKISVRLDKKDGDEAEEKWVLTVGCNLPGNWVLHWGVSYTH 115
Query: 142 DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTV-HEVKIDLVPNKAISAIN 201
D GSEWDQPP EM PPGSIPIKDYAIETPL +SS++ G EV+I+ +ISAIN
Sbjct: 116 DIGSEWDQPPLEMRPPGSIPIKDYAIETPLMESSAALEGQLAQQEVQIEFDTPSSISAIN 175
Query: 202 FVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFN 261
FV KDEETGVW QH+GRDFKVP + +D N +G K+S+G+
Sbjct: 176 FVFKDEETGVWCQHRGRDFKVPLTGKALNDANSIGAKKSIGILP---------------- 235
Query: 262 IGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSV 321
++K S+ +E +GE + ++ LE FYEE I+KE V ++++VSV
Sbjct: 236 ----------VLKEGSSPNVEEKTGEVEEPAQQNMRLEAFYEEYSILKEFPVQSAITVSV 295
Query: 322 RK---------------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQP 381
K D +KWEIP APHP +TT FKNKAL+T LQ
Sbjct: 296 TKSPDSEKNLVNFETNLPGDVVVHWGVCRDSDKKWEIPEAPHPAQTTTFKNKALQTILQL 355
Query: 382 IEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEE 441
E +G G+F + EE G LFVLK + N+WLN G+DFYIP ++ ++ Q +E
Sbjct: 356 KEDGRGSSGVFPVGEELMGLLFVLKLSNNTWLNDMGNDFYIPLATETSIHTQTPAAE--- 415
Query: 442 AGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAA 501
GEGS TD IIKEIR+LVTDI+S++ + ++KEA+E ILQEIEKLAA
Sbjct: 416 -----TGGEGS------VDTDEIIKEIRHLVTDISSEKRWQTKSKEAQEVILQEIEKLAA 475
Query: 502 EAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLK 561
EAY IFRS P F E + + P+KI+SGTG+GFEI+CQGFNWES+KSGRWY L
Sbjct: 476 EAYGIFRSTTPAFVEEVADAEVLEKTPIKITSGTGSGFEIVCQGFNWESNKSGRWYKELL 535
Query: 562 EKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITV 621
+KA EL+S+GFTVIWLPPPT+SVSPEGYMPRDLYNLNSRYG+++ELK ++K FH+VGI
Sbjct: 536 DKANELASIGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSMEELKVLLKKFHEVGIKT 595
Query: 622 LGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNID 681
LGD VLNHRCA +NQNG+WNIFGG+LNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNID
Sbjct: 596 LGDAVLNHRCAQKQNQNGVWNIFGGKLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNID 655
Query: 682 HSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLS 741
HSQ+FVRND+KEWLCWLR EIGY GWRLDFVRGFWGGYVKDY++A+EPYF+VGEYWDSLS
Sbjct: 656 HSQEFVRNDLKEWLCWLRNEIGYHGWRLDFVRGFWGGYVKDYMEATEPYFSVGEYWDSLS 715
Query: 742 YTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPG 801
Y+YGEMDHNQD HRQRI+DWINATNGT+GAFDVTTKGILH+ L+RCEYWRLSD KG+PPG
Sbjct: 716 YSYGEMDHNQDAHRQRIIDWINATNGTSGAFDVTTKGILHATLERCEYWRLSDSKGKPPG 775
Query: 802 VVGWWPSRAVTFIENHDTGSTQ-----------------------------GHWRFPGGK 861
VVGWWPSRAVTFIENHDTGSTQ +FP GK
Sbjct: 776 VVGWWPSRAVTFIENHDTGSTQVGIAYRFIAEITSCSNLIATNTDIVPFPNTLEKFPEGK 835
Query: 862 EMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAA 921
EMQGYAYILTHPGTPSVF+DHIFSH SEIA L+S+R RNK+NCRS + I KAERDVYAA
Sbjct: 836 EMQGYAYILTHPGTPSVFYDHIFSH-KSEIAGLISIRNRNKINCRSTINITKAERDVYAA 895
Query: 922 TIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFR 981
IDEKVAMKIGPGH+EPP+GS W EG DYK +
Sbjct: 896 IIDEKVAMKIGPGHYEPPNGSE-WKSAFEGRDYKTLD----------------------- 955
Query: 982 LRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAV 1041
+K+++TT R +
Sbjct: 956 --------------------------------------KKVSSTTLREEL---------E 1015
Query: 1042 AIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKK 1101
A I FDD+W R EA++ A + P+PE VT N V L G+NSTRR LKK
Sbjct: 1016 KQFQAHIRDFDDHWLQRQDEARRFAMEVYHPNPENVTAHFNEHVRIALEGTNSTRRTLKK 1075
Query: 1102 YTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVN 1161
+TG C ATNPIDRCWRC PNWA++RK+LA CV GFGR T+GGK G YVV + SD+DL N
Sbjct: 1076 HTGRCLATNPIDRCWRCRPNWATDRKRLASCVKGFGRNTVGGKFGRIYVVTNPSDNDLAN 1135
Query: 1162 PKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFI 1221
P+PGTLRHA IQK PLWIIF R+M I L QEL++ S KTID RGANV IAYGAGIT+QF
Sbjct: 1136 PRPGTLRHAAIQKEPLWIIFARSMLITLQQELMVQSHKTIDGRGANVHIAYGAGITVQFA 1195
Query: 1222 NNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDG 1281
NVIIH L IH IV GG IRDSVDH+G RT SDGD +SI+GSSN+W+DH+SMSNC DG
Sbjct: 1196 RNVIIHNLHIHDIVSRNGGLIRDSVDHIGFRTASDGDAVSIYGSSNVWVDHLSMSNCKDG 1255
Query: 1282 LIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCR 1341
L+D I GST IT+SN H THHNEVMLFGA D D IMQVTVAFNH+GQGL+QRMPRCR
Sbjct: 1256 LVDIIEGSTGITVSNNHMTHHNEVMLFGAHDDQPLDQIMQVTVAFNHYGQGLIQRMPRCR 1315
Query: 1342 WGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWT 1400
WGF HVVNNDYTHWLMYAIGGSQHPTI+SQGNRFIAPP AKEVT R +P++VWK WT
Sbjct: 1316 WGFIHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPTQAAKEVTHRSDAPQSVWKDWT 1321
BLAST of Sgr029085 vs. NCBI nr
Match:
XP_038891960.1 (alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1599.7 bits (4141), Expect = 0.0e+00
Identity = 774/915 (84.59%), Postives = 818/915 (89.40%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
MSTI LDPL YHCGK + RFH RPRFNTLK CSFSYCPNKLL HGR+SF NYNSYRLP
Sbjct: 1 MSTISLDPLLYHCGKGKHRFHYRPRFNTLKPCSFSYCPNKLLHHGRKSFVNYNSYRLPTF 60
Query: 68 RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
RAS+TNA LL+TF+SKDVL +ETFP+KR EK+EGRI VRLAQG +Q QWELTVGCNL GK
Sbjct: 61 RASATNAPLLQTFQSKDVLFQETFPLKRNEKLEGRISVRLAQGNDQNQWELTVGCNLAGK 120
Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
WILHWGVSLI DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSS GD VHEVKI+L
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSSGDFVHEVKINL 180
Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
VP+K I+AINFVLKDE+TG+W+QHKGRDFKVP DYHG+DGNIVGTK+ LGLW
Sbjct: 181 VPDKTIAAINFVLKDEQTGIWYQHKGRDFKVPLLDYHGEDGNIVGTKKGLGLWP------ 240
Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
GALGQLSNLLVKAES+SK + SGESGD KEEKKYLEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKAESSSKDKGRSGESGDAKEEKKYLEGFYEELPIVKEV 300
Query: 308 AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
AVDNSVSVSVRK DETRKWEIPAAPHPPETTVFK
Sbjct: 301 AVDNSVSVSVRKCPETTKNLLYLETDLPGDVIVHWGACRDETRKWEIPAAPHPPETTVFK 360
Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
NKALRT LQP EG KGCCGIFT+EE+F GFLFVLKQNENSWLNYKGDDFYIP S+S NLP
Sbjct: 361 NKALRTLLQPKEGGKGCCGIFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLSNSGNLP 420
Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
QQR+SEL++AGVS K+GE SEGVS AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+ES
Sbjct: 421 GQQRESELKDAGVSKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQES 480
Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
ILQEIEKLAAEAYSIFRS+APTFTE +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSSAPTFTEEVIEMPKPVEPPVQISSGTGSGFEILCQGFNWESH 540
Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
KSGRWYM LKE+AAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKERAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600
Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
KTFHDVGI VLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660
Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720
Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780
Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTPS F+
Sbjct: 781 LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPCGKEMQGYAYILTHPGTPSAFY 840
Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 896
DHIFSHY SEIAAL+SLRKRNKVNCRSVV IEKAERDVYAA IDEKVA+KIGPG+FEPPS
Sbjct: 841 DHIFSHYKSEIAALISLRKRNKVNCRSVVTIEKAERDVYAAIIDEKVAVKIGPGNFEPPS 898
BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match:
Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)
HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/857 (66.28%), Postives = 666/857 (77.71%), Query Frame = 0
Query: 66 APRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLT 125
A RA+S++ A++ET +S DV+ KE FP++R EK EG+I+VRL + K +K WEL+VGC++
Sbjct: 63 AIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVK-EKNWELSVGCSIP 122
Query: 126 GKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKI 185
GKWILHWGVS +GD+GSEWDQPP++M PPGSI IKDYAIETPLKK S GD+ EV I
Sbjct: 123 GKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEVAI 182
Query: 186 DLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVY 245
+L +++A+NFVLKDEETG W+QHKGRDFKVP D D+GN++G K+
Sbjct: 183 NLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKK---------- 242
Query: 246 HVGGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVK 305
GALGQLSN+ +K Q+ S D+ EE+K L+ FYEE+PI K
Sbjct: 243 -----------GFGALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISK 302
Query: 306 EVAVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTV 365
VA DNSVSV+ R K+ T+KWEIP+ P+P ET++
Sbjct: 303 RVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSL 362
Query: 366 FKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVN 425
FKNKALRT LQ + G G+F+L+ + EG FVLK NEN+WLNY+G+DFY+PF +S +
Sbjct: 363 FKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSS 422
Query: 426 LPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAK 485
P E E A VS + + VS +T II EIRNL DI+S + QK KE +
Sbjct: 423 SP-----VETEAAQVSKPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQ 482
Query: 486 ESILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWE 545
E+ILQEIEKLAAEAYSIFRS P F+E + + +P +KISSGTG+GFEILCQGFNWE
Sbjct: 483 ENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWE 542
Query: 546 SHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKD 605
S+KSGRWY+ L+EKA EL+SLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYG IDELKD
Sbjct: 543 SNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKD 602
Query: 606 VVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKS 665
VK FH VGI VLGD VLNHRCAHFKNQNG+WN+FGGRLNWDDRAVV+DDPHFQGRGNKS
Sbjct: 603 TVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKS 662
Query: 666 SGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEP 725
SGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+P
Sbjct: 663 SGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKP 722
Query: 726 YFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEY 785
YFAVGEYWDSLSYTYGEMD+NQD HRQRIVDWINAT+G AGAFDVTTKGILH+AL +CEY
Sbjct: 723 YFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEY 782
Query: 786 WRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSV 845
WRLSD KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+V
Sbjct: 783 WRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAV 842
Query: 846 FFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEP 896
FFDHIFS Y+SEIAAL+SLR R K++CRS V I+K+ERDVYAA IDEKVAMKIGPGH+EP
Sbjct: 843 FFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEP 882
BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match:
P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)
HSP 1 Score: 590.1 bits (1520), Expect = 6.5e-167
Identity = 289/438 (65.98%), Postives = 332/438 (75.80%), Query Frame = 0
Query: 973 LLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVL--- 1032
L +T + + A IG +D+ W+ RA EA++ A +EPHPE VT + N ++ +
Sbjct: 13 LCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKEL 72
Query: 1033 -----TGSNSTRRNL--KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIG 1092
T +NSTRR L KKYTGPC TNPID+CWRCDPNWA NRKKLADC +GFG K IG
Sbjct: 73 KKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIG 132
Query: 1093 GKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTID 1152
GK G +YVV D+SD D +PKPGTLRHAVIQK PLWIIF R M IRL QE+IM SDKTID
Sbjct: 133 GKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTID 192
Query: 1153 ARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISI 1212
ARG NV I GAGITLQ+I NVIIHGL IH IV G GG +RD+VDH+G+RT SDGDGISI
Sbjct: 193 ARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISI 252
Query: 1213 FGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQV 1272
FG+S IWIDHVSM C+DGLIDA+ GST ITISN HFT HNEVMLFGASDS S D +MQ+
Sbjct: 253 FGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQI 312
Query: 1273 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVY 1332
T+AFNHFG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII QGNRFIAPP+++
Sbjct: 313 TLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIF 372
Query: 1333 AKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL-DMISYKPGTFV 1392
K+VTKREY+PE+VW WTWRSEG+L MNGA+F SGD S L D IS P V
Sbjct: 373 KKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDV 432
Query: 1393 TRMTRFAGALPCFVGRPC 1400
T MTRFAG L C G+PC
Sbjct: 433 TWMTRFAGVLGCKPGKPC 449
BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match:
P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)
HSP 1 Score: 551.6 bits (1420), Expect = 2.6e-155
Identity = 268/421 (63.66%), Postives = 319/421 (75.77%), Query Frame = 0
Query: 980 AVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL 1039
+ A+++A+I FD+YW+ ++ AQ A A+ PHPE VT+ N V G NSTRRNL
Sbjct: 16 SAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNL 75
Query: 1040 K-KYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSD 1099
+ G C ATNPIDRCWRC NW++NRK L CV GFGRKT GG G YVV D SD
Sbjct: 76 RTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDS 135
Query: 1100 LVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITL 1159
L +PK GTLR VIQ PLWIIF ++M IRL QELI+ +DKTID RGANVQIA GA +T+
Sbjct: 136 LTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTV 195
Query: 1160 QFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNC 1219
QF++NVIIHG+ IH I G GG IRDS H G+RT SDGDGISI GSSNIWIDHVS++ C
Sbjct: 196 QFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARC 255
Query: 1220 HDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMP 1279
DGLID I+GSTAITISNCH T H++VML GASD+Y+ D IMQVTVAFNHFG+GLVQRMP
Sbjct: 256 SDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMP 315
Query: 1280 RCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWK 1339
RCR+GF HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IAP AKEVTKR+Y+ W
Sbjct: 316 RCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWS 375
Query: 1340 SWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRP 1399
WTW+S+GDL ++GAFFV SG +++ +S+ D+I KPGTFV R+TRF+GAL C
Sbjct: 376 KWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENME 434
BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match:
Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)
HSP 1 Score: 541.6 bits (1394), Expect = 2.7e-152
Identity = 265/440 (60.23%), Postives = 313/440 (71.14%), Query Frame = 0
Query: 977 LSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGS---N 1036
L++ ++ VFD YW R +A K +++PHP VT+ N V + S N
Sbjct: 20 LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79
Query: 1037 STRRNL---------KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGK 1096
TRR L K +G C A NPID CWRCD NWA+NRKKLADCVLGFGR+T GGK
Sbjct: 80 DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139
Query: 1097 LGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDAR 1156
GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF R+M I+L QEL++TSDKTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199
Query: 1157 GANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFG 1216
GA V I GAG+TLQF+NNVIIH + + HIV G GG IRDS H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259
Query: 1217 SSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTV 1276
++NIWIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D + D MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319
Query: 1277 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAK 1336
AFNHFG+ L QRMPRCR+G HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN AK
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379
Query: 1337 EVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMISYKPGT 1396
++TKREY+P WKSW W+SEGD +NGA+FV SG + P I KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439
Query: 1397 FVTRMTRFAGALPCFVGRPC 1400
V ++T AG L C +G C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459
BLAST of Sgr029085 vs. ExPASy Swiss-Prot
Match:
Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)
HSP 1 Score: 530.4 bits (1365), Expect = 6.1e-149
Identity = 260/467 (55.67%), Postives = 323/467 (69.16%), Query Frame = 0
Query: 970 MVCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE----- 1029
+VC++ SL I D+YW +A EA+K A+ P P + D +
Sbjct: 9 LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68
Query: 1030 -------------------VGQVLTGSNSTRRNL------------KKYTGPCRATNPID 1089
+ + + +NSTRR+L K TGPC A+NPID
Sbjct: 69 TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128
Query: 1090 RCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQ 1149
+CWRC P+WA RKKL CV GFG +T GGK G YVV D D+VNP+PGTLRHAVIQ
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188
Query: 1150 KGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHH 1209
K PLWI+F +M+IRL+QEL++TSDKTIDARGANV IAYGAGIT+Q+++N+IIHGL +HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248
Query: 1210 IVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAIT 1269
IV +GG IRDS++H G R +DGDGISIFG++NIW+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308
Query: 1270 ISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 1329
ISN HFTHHN+VML GA ++ D MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368
Query: 1330 HWLMYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNG 1389
HW +YAIGGSQ PTI+S GNRFIAPP+ + +EVTKR+Y+ E+ WK+W WRSE D+ MN
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428
Query: 1390 AFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
A+F SG+ SR MI K G V+++T++AGAL C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475
BLAST of Sgr029085 vs. ExPASy TrEMBL
Match:
A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)
HSP 1 Score: 2378.6 bits (6163), Expect = 0.0e+00
Identity = 1157/1419 (81.54%), Postives = 1228/1419 (86.54%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
MS+I LDPL YHC K + RFH RPRFN LK CSF+YCPNKLLCHGR++F +YNSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 68 RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
+A++TNA TF+S DVL KETFP+KR EK+EGRI V+LAQGK+ +WELTVGCNL GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
WILHWGVSLI DSGSEWDQPPKEMIPPGSI IKDYAIETPLKKSSSSS GD VHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
P+K I+AINFVLKDEETG+W+QHKGRDFKVP DY G+DGNIVG K+ LGLW
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
GALGQLSNLLVK E+ SK Q SS ESGDTKE+KK LEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKSLEGFYEELPIVKEV 300
Query: 308 AVDNSVSVSVRK---------------------------DETRKWEIPAAPHPPETTVFK 367
AVDNS+SVSV+K D+TRKWEIPAAPHPPETTVFK
Sbjct: 301 AVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWEIPAAPHPPETTVFK 360
Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
NKALRT LQP EG KGC G+FT+EE+F GFLFVLKQNENSWLNYKGDDFYIP SS NLP
Sbjct: 361 NKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKGDDFYIPLPSSGNLP 420
Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
DQQR+S+L+++G S K+GE SEGVS AYTDGIIKEIRNLVTDI+SQ+T+KK+TKEA+E+
Sbjct: 421 DQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKTKKKKTKEAQEN 480
Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
ILQEIEKLAAEAYSIFRS+APTFTE +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGSGFEILCQGFNWESH 540
Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
KSGRWYM LKEKAAELSSLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDVV 600
Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
KTFHDVGI VLGD VLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660
Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720
Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
AVGEYWDSLSYTYGEMDHNQD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780
Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
LSD+KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY+LTHPGTPSVF+
Sbjct: 781 LSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYLLTHPGTPSVFY 840
Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 907
DHIFSHY SEIAAL+SLRKRNKVNCRSVVKI KAERDVYAATIDE VA+KIGPG+FEPPS
Sbjct: 841 DHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAVKIGPGNFEPPS 900
Query: 908 GSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHY 967
GSSGWSLV EG DY K EDNAAIGL IGDLYCSI + R PIT R
Sbjct: 901 GSSGWSLVIEGKDY-KTEDNAAIGLCIGDLYCSICWLNPTRTFHQPITMAR--------- 960
Query: 968 TILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAVAIANADIGVFDDYWKARAI 1027
+ LLLTLSL A ADIG FDDYWKARA
Sbjct: 961 ------------------------------LSLLLTLSLLATTAFADIGDFDDYWKARAA 1020
Query: 1028 EAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1087
EA++AAA A+EP+PE + DSLNAEVGQ L+GSNSTRRNLKKYTGPCRATNPIDRCWRCDP
Sbjct: 1021 EAEQAAADAYEPNPETIADSLNAEVGQALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDP 1080
Query: 1088 NWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWII 1147
NWA NRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDL+NPKPGTLRHAVIQKGPLWI
Sbjct: 1081 NWAQNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWIT 1140
Query: 1148 FDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGG 1207
F NMAIRLTQELI+TSDKTIDARGANVQIAYGAGITLQ++ NVIIHGLRIHHIVVG GG
Sbjct: 1141 FSTNMAIRLTQELIVTSDKTIDARGANVQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGG 1200
Query: 1208 TIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFT 1267
IRD+VDHVGLRTMSDGDGISIFGSSN+WIDHVSMSNC DGLIDAIMGSTAITISNCHFT
Sbjct: 1201 MIRDAVDHVGLRTMSDGDGISIFGSSNVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFT 1260
Query: 1268 HHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1327
HHNEVMLFGASD YS D IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI
Sbjct: 1261 HHNEVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 1320
Query: 1328 GGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGD 1387
GGSQHPTI+SQGNRFIAPPNVYAKEVTKREYSPE VWKSWTWRSEGDLMMNGAFFVTSGD
Sbjct: 1321 GGSQHPTIVSQGNRFIAPPNVYAKEVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGD 1359
Query: 1388 QRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
QRK RPFS DMISYKPGT+V RMTRF+GAL CFVGRPC
Sbjct: 1381 QRKRRPFSSKDMISYKPGTYVKRMTRFSGALACFVGRPC 1359
BLAST of Sgr029085 vs. ExPASy TrEMBL
Match:
A0A498HGM8 (Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_007136 PE=3 SV=1)
HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 906/1469 (61.67%), Postives = 1061/1469 (72.23%), Query Frame = 0
Query: 8 MSTIGLDPL---FYHCGKWEKRFHQRPRFN-TLK-TCSFSYCPNKLLCHGRRSFFNYNSY 67
MST+ ++PL +H G+ +K H+RP+ N LK + SF+ P KL+ RSF +
Sbjct: 13 MSTVRIEPLLQDLHHYGR-QKPSHRRPQSNHPLKLSSSFTAFPKKLVVSNSRSFCYFQP- 72
Query: 68 RLPAP--------RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQK 127
P P RA+ST+ +ET +S D + K+TFP+KRTE VEG+IFV+L GKN+K
Sbjct: 73 --PTPRRGPTLGVRAASTDTTTVETSESADPIYKKTFPLKRTEVVEGKIFVKLDHGKNEK 132
Query: 128 QWELTVGCNLTGKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------- 187
+W LTVGCNL GKW+LHWGVS + D EW+QPP EM P GS+PIK
Sbjct: 133 KWVLTVGCNLPGKWVLHWGVSFVDDVSCEWEQPPSEMRPAGSVPIKVCNWVLKFDCRAVG 192
Query: 188 -----------DYAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWF 247
DYAIETPLK+S SS GDT +EVKID+ PN AI+AINFVLKDEETG W+
Sbjct: 193 WNEFDIHEDFQDYAIETPLKESLSSVGGDTSYEVKIDVKPNSAIAAINFVLKDEETGAWY 252
Query: 248 QHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLV 307
QH+G DF+VP Y DD N+VG + G+W G LG+LSN+ V
Sbjct: 253 QHRGSDFRVPLVAYPQDDDNVVGATKGFGMWP-----------------GVLGKLSNVFV 312
Query: 308 KAEST-SKSQESSG-ESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK-------- 367
KAES+ SK Q+SSG ES +++ +EGFYEELPI KE++V+NSV+VSV K
Sbjct: 313 KAESSNSKDQDSSGSESRHPQQKTTCVEGFYEELPIAKEISVNNSVTVSVSKCPETAKNL 372
Query: 368 -------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGI 427
D+T+ WEIPAAPHPPET VFK+KALRT LQ EG GC G+
Sbjct: 373 LYLETDLPDNAVVHWGVCRDDTKTWEIPAAPHPPETVVFKDKALRTRLQQKEGGNGCWGL 432
Query: 428 FTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEG 487
FTLEE GFLF+LK NE++WL G+DFYIP SSS N Q + + ++A V + E
Sbjct: 433 FTLEEGLAGFLFLLKLNESTWLRCVGNDFYIPLSSSKNANVVQSEIQSKDAQVPDGSTEA 492
Query: 488 SEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAA 547
E AY DG+I E+RNLV+D+ S ++ + R+K+A+E+ILQEIEKLAAEAYSIFR+
Sbjct: 493 VE--ESTAYADGLINEMRNLVSDVFSDKSPRTRSKKAQEAILQEIEKLAAEAYSIFRTTV 552
Query: 548 PTFTEITL-ETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSL 607
PT E T+ ET + P KI SGTGTGFEILCQGFNWES KSGRWYM LK KAA LSSL
Sbjct: 553 PTLPEETIAETEEVKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYMELKSKAALLSSL 612
Query: 608 GFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHR 667
GFTVIW PPPTESVSPEGYMPRDLYNLNSRYGN+DELK+ VK FHDVGI VLGD VLNHR
Sbjct: 613 GFTVIWFPPPTESVSPEGYMPRDLYNLNSRYGNMDELKETVKVFHDVGIKVLGDAVLNHR 672
Query: 668 CAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRND 727
CAH++NQNG+WNIFGGRLNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR D
Sbjct: 673 CAHYRNQNGVWNIFGGRLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKD 732
Query: 728 IKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHN 787
I+EWLCWLR +IGYDGWRLDFVRGFWGGYV+DY+DASEPYFAVGEYWDSLSYTYGEMD N
Sbjct: 733 IREWLCWLRNDIGYDGWRLDFVRGFWGGYVRDYVDASEPYFAVGEYWDSLSYTYGEMDRN 792
Query: 788 QDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRA 847
QD HRQRIVDWINATNGT GAFDVTTKGILH+AL+RCEYWRLSD+KG+PPGV+GWWPSRA
Sbjct: 793 QDAHRQRIVDWINATNGTCGAFDVTTKGILHAALERCEYWRLSDEKGKPPGVLGWWPSRA 852
Query: 848 VTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRK 907
VTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR
Sbjct: 853 VTFIENHDTGSTQGHWRFPKGKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRN 912
Query: 908 RNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAED 967
RNK+NCRS+VKI KAERDVYAA IDEKVA+KIGPGH+EP SG W+ EG DYK
Sbjct: 913 RNKLNCRSLVKITKAERDVYAAIIDEKVAIKIGPGHYEPASGPQNWNKSLEGGDYK---- 972
Query: 968 NAAIGLWIGDLYCSISNPSVFRLRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHP 1027
+W L S S G + P
Sbjct: 973 -----VWEASLVFSAPTRST------------------------------EDGAGGADRP 1032
Query: 1028 RKMAATTNRNMVCLL----LTLSLAVAIANADIGV--FDDYWKARAIEAQKAAATAFEPH 1087
++ C + N D V D YWK +A EA+K A ++ +
Sbjct: 1033 ASAVQNRGQSAECGSGEHGFCNDNPTDVPNEDTLVLDLDAYWKEQAEEAEKVAMESYNKN 1092
Query: 1088 PEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASNRKKLA 1147
PE VT+ N EVG++++ N+TRRNL K YTGPC ATNP+D CWRCDPNWA+NRKK+
Sbjct: 1093 PEKVTEEFNTEVGKLMSKENTTRRNLRGNKAYTGPCMATNPMDACWRCDPNWANNRKKIV 1152
Query: 1148 DCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLT 1207
C GFG+KT GGK GP+YVV +D D+ NP PGTLRHAVIQKGPLWIIF ++M IRL
Sbjct: 1153 GCAQGFGKKTTGGKDGPFYVVTMGTDDDVQNPTPGTLRHAVIQKGPLWIIFTKSMVIRLQ 1212
Query: 1208 QELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVG 1267
QEL++TSDKTIDARGANV I GAGITLQF+ NVII L I IV GG IRDSVDH+G
Sbjct: 1213 QELMVTSDKTIDARGANVVIEEGAGITLQFVKNVIITNLHIKMIVTKPGGLIRDSVDHIG 1272
Query: 1268 LRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGA 1327
LRT SDGDGIS+FG+SN+WIDHVSMS C DGLIDAIMGSTAITISN HFT H+E MLFGA
Sbjct: 1273 LRTQSDGDGISLFGASNVWIDHVSMSRCADGLIDAIMGSTAITISNSHFTDHDEAMLFGA 1332
Query: 1328 SDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 1387
+++++ D IMQ+T+AFNHFGQGLVQRMPR R GFFHVVNNDYTHW+MYAIGG+ +PTIIS
Sbjct: 1333 NNAHTQDKIMQITLAFNHFGQGLVQRMPRVRHGFFHVVNNDYTHWIMYAIGGNMNPTIIS 1392
Query: 1388 QGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSRPFSRL 1400
QGNRFIAP N K+VT RE +PE WK+W WRSEGDLMMNGAFFV SG P ++L
Sbjct: 1393 QGNRFIAPINGVTKQVTHRENTPEAEWKNWEWRSEGDLMMNGAFFVESGSGASKHP-AKL 1418
BLAST of Sgr029085 vs. ExPASy TrEMBL
Match:
A0A498IC60 (Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_040744 PE=3 SV=1)
HSP 1 Score: 1711.4 bits (4431), Expect = 0.0e+00
Identity = 881/1468 (60.01%), Postives = 1039/1468 (70.78%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLK-TCSFSYCPNKLLCHGRRSFFNYNSYRLPA 67
MST+ ++PL +H + + P + LK + SF+ P KL+ RSF N+ L +
Sbjct: 1 MSTVRIEPLLHHYRRQKPSHRLPPSKHPLKLSSSFTAFPKKLVVSNGRSFCNFQPPTL-S 60
Query: 68 PRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTG 127
RA+ST+ A +E + D KETFP+KRTE VEG++ V+L GK+ K W LTVGCNL G
Sbjct: 61 VRAASTDTATVEATEFADAFYKETFPLKRTEVVEGKMIVKLDNGKDAKNWVLTVGCNLPG 120
Query: 128 KWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIK-------------------------D 187
KW+LHWGV+ + D GSEWDQPP EM P GS+ IK D
Sbjct: 121 KWVLHWGVNYVDDVGSEWDQPPSEMRPAGSVSIKVRIWVLKFDCRAVGWHEFNKRVDFQD 180
Query: 188 YAIETPLKKSSSSSLGDTVHEVKIDLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFS 247
YAIETPLK+S S GDT HEVKID+ PN AI+AINFVLKDEETG W+QH+GRDFKVPF
Sbjct: 181 YAIETPLKESLSPVGGDTSHEVKIDVTPNSAIAAINFVLKDEETGAWYQHRGRDFKVPFV 240
Query: 248 DYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFNIGALGQLSNLLVKAE-STSKSQES 307
Y DD N+VG R+LG W G LG+LSN+ VKAE S SK QES
Sbjct: 241 CYLQDDDNVVGATRALGAWS-----------------GTLGKLSNVFVKAETSNSKDQES 300
Query: 308 SGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSVRK--------------------- 367
S ES D +++ LEGFYEELPI KE+AV++S +VSVRK
Sbjct: 301 SSESRDPQQKTMRLEGFYEELPIAKEIAVNHSATVSVRKCPETTKNLLYLETDLPDHAVV 360
Query: 368 ------DETRKWEIPAAPHPPETTVFKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFV 427
D+ ++WEIPAAPHPPET VFK+KALRT LQ E GC G+FTLEE GFLFV
Sbjct: 361 HWGVCRDDAKRWEIPAAPHPPETVVFKDKALRTRLQQREDGNGCSGLFTLEEGLAGFLFV 420
Query: 428 LKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGI 487
K NE WLN G+DFYIP SS N Q + + E+A V ++ E + AYTDGI
Sbjct: 421 FKLNETMWLNCVGNDFYIPLLSSNNSIAVQSEVQSEDAQVPDRSRE----TNFTAYTDGI 480
Query: 488 IKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAAEAYSIFRSAAPTF-TEITLETPK 547
I EIRNLV+DI+S+++Q+KR+KEA+E+ILQEIEKLAAEAYSIFR+ PT EI ET K
Sbjct: 481 INEIRNLVSDISSEKSQRKRSKEAQETILQEIEKLAAEAYSIFRTTVPTLPEEIIAETEK 540
Query: 548 PVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTES 607
P KI SGTGTGFEILCQGFNWES KSGRWY LK KAAELSSLGFTVIW PPPT+S
Sbjct: 541 VKVAPAKICSGTGTGFEILCQGFNWESSKSGRWYEELKSKAAELSSLGFTVIWFPPPTDS 600
Query: 608 VSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNI 667
VSP+GYMPRDLYN+NSRYGN+DELK+ VKTFHD G+ VLGD VLNHRCA ++NQNG+WNI
Sbjct: 601 VSPQGYMPRDLYNMNSRYGNMDELKETVKTFHDAGLKVLGDAVLNHRCAEYQNQNGVWNI 660
Query: 668 FGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIG 727
FGGRLNWD+RAVV+DDPHFQGRGNKSSGD+FHAAPNIDHSQDFVR DI+EWLCWLR +IG
Sbjct: 661 FGGRLNWDERAVVADDPHFQGRGNKSSGDSFHAAPNIDHSQDFVRKDIREWLCWLRDDIG 720
Query: 728 YDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWIN 787
YDGWRLDFVRGFWGGYVKDY+DASEPYFAVGEYWDSLSYTYGEMDHNQD HRQRIVDWIN
Sbjct: 721 YDGWRLDFVRGFWGGYVKDYMDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWIN 780
Query: 788 ATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQ 847
ATNGT GAFDVTTKGILH+AL+RCEYWRLSD KG+PPGV+GWWPSRAVTFIENHDTGSTQ
Sbjct: 781 ATNGTCGAFDVTTKGILHAALERCEYWRLSDTKGKPPGVLGWWPSRAVTFIENHDTGSTQ 840
Query: 848 GHWRFPGGKEMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIE 907
GHWRFP KEMQGYAYILTHPGTP+VF+DHIFSHY SEIAAL+SLR RNK+NCRS VK++
Sbjct: 841 GHWRFPNKKEMQGYAYILTHPGTPTVFYDHIFSHYQSEIAALISLRNRNKLNCRSRVKLK 900
Query: 908 KAERDVYAATIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYC 967
+ T MK P + + LV K + +
Sbjct: 901 SPRQ---KETSMRPSLMKRSPSKSDQVITN---LLVDLKIGIKALREETTRSGKRHKFFL 960
Query: 968 SISNPSVFRLRLN-------PITSPR-----FKAWPDNHYTILELSFLFSWGVKESLHPR 1027
P++ R + P ++ + + H + ++S V P
Sbjct: 961 HRHFPNLRDCRRSTEDETGRPASAAQNRGQSAECGSGEHVSSRKVS------VAADEVPT 1020
Query: 1028 KMAATTNRNMVCL-LLTLSLAV---------AIANADIGV--FDDYWKARAIEAQKAAAT 1087
MAA N + L L LS AV +AN D V D YWK RA EA K A
Sbjct: 1021 TMAAMFNSSYFFLCLFFLSFAVFVSSNDGPTDVANEDSTVLDLDAYWKERAQEAFKEAME 1080
Query: 1088 AFEPHPEAVTDSLNAEVGQVLTGSNSTRRNL---KKYTGPCRATNPIDRCWRCDPNWASN 1147
++ +PE VT+ N V ++L+ N+TRRNL K Y GPC ATNPID CWRCDPNWA N
Sbjct: 1081 SYHKNPEQVTEEFNTGVAELLSKENTTRRNLRGNKGYNGPCMATNPIDACWRCDPNWADN 1140
Query: 1148 RKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNM 1207
RKK+ C GFG+K GGK GP YVV +D D+ NPKPGTLRHAVIQKGPLWIIF R+M
Sbjct: 1141 RKKIVGCAQGFGKKATGGKDGPIYVVTVGTDDDVQNPKPGTLRHAVIQKGPLWIIFARSM 1200
Query: 1208 AIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDS 1267
IRL QEL++TSDKTID RGANV I G GITLQF+ NVI+ L I IV GG IRDS
Sbjct: 1201 VIRLQQELMVTSDKTIDGRGANVVIEEGGGITLQFVKNVILTNLHIKMIVSKPGGMIRDS 1260
Query: 1268 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEV 1327
VDH+GLRT SDGDGIS+FG+SN+WIDH+SMS C DGLIDA+M STAITISN HFT H++V
Sbjct: 1261 VDHMGLRTKSDGDGISLFGASNVWIDHMSMSRCADGLIDAVMASTAITISNSHFTDHDDV 1320
Query: 1328 MLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 1387
MLFGA+++++ D IMQ+T+AFNHFG+GLVQRMPR R+GFFH+VNNDY HW+MYAIGG+ +
Sbjct: 1321 MLFGANNAHTQDKIMQITLAFNHFGRGLVQRMPRVRYGFFHLVNNDYNHWIMYAIGGNMN 1380
Query: 1388 PTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSGDQRKSR 1394
PTIISQGNRFIAP N K+VT RE++PE+ WK+W WRSEGDLMMNGAFFV SG +
Sbjct: 1381 PTIISQGNRFIAPVNEATKQVTHREHTPESEWKNWEWRSEGDLMMNGAFFVESGSRASQN 1433
BLAST of Sgr029085 vs. ExPASy TrEMBL
Match:
A0A4Y7JLT3 (Pectate lyase OS=Papaver somniferum OX=3469 GN=C5167_022499 PE=3 SV=1)
HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 810/1378 (58.78%), Postives = 960/1378 (69.67%), Query Frame = 0
Query: 82 SKDVLLKETFPIKRTEKVEGRIFVRLAQ---GKNQKQWELTVGCNLTGKWILHWGVSLIG 141
+++VL+ +TFP+ T++VEG+I VRL + + +++W LTVGCNL G W+LHWGVS
Sbjct: 56 TENVLISKTFPLNWTQRVEGKISVRLDKKDGDEAEEKWVLTVGCNLPGNWVLHWGVSYTH 115
Query: 142 DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTV-HEVKIDLVPNKAISAIN 201
D GSEWDQPP EM PPGSIPIKDYAIETPL +SS++ G EV+I+ +ISAIN
Sbjct: 116 DIGSEWDQPPLEMRPPGSIPIKDYAIETPLMESSAALEGQLAQQEVQIEFDTPSSISAIN 175
Query: 202 FVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHVGGMHFLNFFN 261
FV KDEETGVW QH+GRDFKVP + +D N +G K+S+G+
Sbjct: 176 FVFKDEETGVWCQHRGRDFKVPLTGKALNDANSIGAKKSIGILP---------------- 235
Query: 262 IGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEVAVDNSVSVSV 321
++K S+ +E +GE + ++ LE FYEE I+KE V ++++VSV
Sbjct: 236 ----------VLKEGSSPNVEEKTGEVEEPAQQNMRLEAFYEEYSILKEFPVQSAITVSV 295
Query: 322 RK---------------------------DETRKWEIPAAPHPPETTVFKNKALRTPLQP 381
K D +KWEIP APHP +TT FKNKAL+T LQ
Sbjct: 296 TKSPDSEKNLVNFETNLPGDVVVHWGVCRDSDKKWEIPEAPHPAQTTTFKNKALQTILQL 355
Query: 382 IEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLPDQQRQSELEE 441
E +G G+F + EE G LFVLK + N+WLN G+DFYIP ++ ++ Q +E
Sbjct: 356 KEDGRGSSGVFPVGEELMGLLFVLKLSNNTWLNDMGNDFYIPLATETSIHTQTPAAE--- 415
Query: 442 AGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKESILQEIEKLAA 501
GEGS TD IIKEIR+LVTDI+S++ + ++KEA+E ILQEIEKLAA
Sbjct: 416 -----TGGEGS------VDTDEIIKEIRHLVTDISSEKRWQTKSKEAQEVILQEIEKLAA 475
Query: 502 EAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESHKSGRWYMVLK 561
EAY IFRS P F E + + P+KI+SGTG+GFEI+CQGFNWES+KSGRWY L
Sbjct: 476 EAYGIFRSTTPAFVEEVADAEVLEKTPIKITSGTGSGFEIVCQGFNWESNKSGRWYKELL 535
Query: 562 EKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVVKTFHDVGITV 621
+KA EL+S+GFTVIWLPPPT+SVSPEGYMPRDLYNLNSRYG+++ELK ++K FH+VGI
Sbjct: 536 DKANELASIGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSMEELKVLLKKFHEVGIKT 595
Query: 622 LGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNID 681
LGD VLNHRCA +NQNG+WNIFGG+LNWDDRAVV+DDPHFQGRGNKSSGD+FHAAPNID
Sbjct: 596 LGDAVLNHRCAQKQNQNGVWNIFGGKLNWDDRAVVADDPHFQGRGNKSSGDSFHAAPNID 655
Query: 682 HSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLS 741
HSQ+FVRND+KEWLCWLR EIGY GWRLDFVRGFWGGYVKDY++A+EPYF+VGEYWDSLS
Sbjct: 656 HSQEFVRNDLKEWLCWLRNEIGYHGWRLDFVRGFWGGYVKDYMEATEPYFSVGEYWDSLS 715
Query: 742 YTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWRLSDQKGQPPG 801
Y+YGEMDHNQD HRQRI+DWINATNGT+GAFDVTTKGILH+ L+RCEYWRLSD KG+PPG
Sbjct: 716 YSYGEMDHNQDAHRQRIIDWINATNGTSGAFDVTTKGILHATLERCEYWRLSDSKGKPPG 775
Query: 802 VVGWWPSRAVTFIENHDTGSTQ-----------------------------GHWRFPGGK 861
VVGWWPSRAVTFIENHDTGSTQ +FP GK
Sbjct: 776 VVGWWPSRAVTFIENHDTGSTQVGIAYRFIAEITSCSNLIATNTDIVPFPNTLEKFPEGK 835
Query: 862 EMQGYAYILTHPGTPSVFFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAA 921
EMQGYAYILTHPGTPSVF+DHIFSH SEIA L+S+R RNK+NCRS + I KAERDVYAA
Sbjct: 836 EMQGYAYILTHPGTPSVFYDHIFSH-KSEIAGLISIRNRNKINCRSTINITKAERDVYAA 895
Query: 922 TIDEKVAMKIGPGHFEPPSGSSGWSLVSEGNDYKKAEDNAAIGLWIGDLYCSISNPSVFR 981
IDEKVAMKIGPGH+EPP+GS W EG DYK +
Sbjct: 896 IIDEKVAMKIGPGHYEPPNGSE-WKSAFEGRDYKTLD----------------------- 955
Query: 982 LRLNPITSPRFKAWPDNHYTILELSFLFSWGVKESLHPRKMAATTNRNMVCLLLTLSLAV 1041
+K+++TT R +
Sbjct: 956 --------------------------------------KKVSSTTLREEL---------E 1015
Query: 1042 AIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGSNSTRRNLKK 1101
A I FDD+W R EA++ A + P+PE VT N V L G+NSTRR LKK
Sbjct: 1016 KQFQAHIRDFDDHWLQRQDEARRFAMEVYHPNPENVTAHFNEHVRIALEGTNSTRRTLKK 1075
Query: 1102 YTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVN 1161
+TG C ATNPIDRCWRC PNWA++RK+LA CV GFGR T+GGK G YVV + SD+DL N
Sbjct: 1076 HTGRCLATNPIDRCWRCRPNWATDRKRLASCVKGFGRNTVGGKFGRIYVVTNPSDNDLAN 1135
Query: 1162 PKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFI 1221
P+PGTLRHA IQK PLWIIF R+M I L QEL++ S KTID RGANV IAYGAGIT+QF
Sbjct: 1136 PRPGTLRHAAIQKEPLWIIFARSMLITLQQELMVQSHKTIDGRGANVHIAYGAGITVQFA 1195
Query: 1222 NNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDG 1281
NVIIH L IH IV GG IRDSVDH+G RT SDGD +SI+GSSN+W+DH+SMSNC DG
Sbjct: 1196 RNVIIHNLHIHDIVSRNGGLIRDSVDHIGFRTASDGDAVSIYGSSNVWVDHLSMSNCKDG 1255
Query: 1282 LIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCR 1341
L+D I GST IT+SN H THHNEVMLFGA D D IMQVTVAFNH+GQGL+QRMPRCR
Sbjct: 1256 LVDIIEGSTGITVSNNHMTHHNEVMLFGAHDDQPLDQIMQVTVAFNHYGQGLIQRMPRCR 1315
Query: 1342 WGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAKEVTKREYSPENVWKSWT 1400
WGF HVVNNDYTHWLMYAIGGSQHPTI+SQGNRFIAPP AKEVT R +P++VWK WT
Sbjct: 1316 WGFIHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPTQAAKEVTHRSDAPQSVWKDWT 1321
BLAST of Sgr029085 vs. ExPASy TrEMBL
Match:
A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)
HSP 1 Score: 1595.1 bits (4129), Expect = 0.0e+00
Identity = 783/915 (85.57%), Postives = 818/915 (89.40%), Query Frame = 0
Query: 8 MSTIGLDPLFYHCGKWEKRFHQRPRFNTLKTCSFSYCPNKLLCHGRRSFFNYNSYRLPAP 67
MSTIGLDPL YHCGK + RFH+RP+FNTLK CSF Y PNKLL GRRSFFN NSYRLPA
Sbjct: 1 MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60
Query: 68 RASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLTGK 127
R SSTNAALLE+F+S DVLLKETFP+KRTEKVEG IFVRLAQGKNQ QWELTVGCNLTGK
Sbjct: 61 RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120
Query: 128 WILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 187
WILHWGVSLI DSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180
Query: 188 VPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVYHV 247
VPNK I+AINFVLKDEETG+W+QHKGRDFKVP +D HGDDGNIVGTK+ LGLW
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWP------ 240
Query: 248 GGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVKEV 307
GALGQLSNLLVKAES SK QESSGESGDTKE KYLEGFYEELPIVKEV
Sbjct: 241 -----------GALGQLSNLLVKAES-SKGQESSGESGDTKE--KYLEGFYEELPIVKEV 300
Query: 308 AVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTVFK 367
A DNSVSVSVR +DETRKWEIPAAPHPPETTVFK
Sbjct: 301 AFDNSVSVSVRNCPETTKYLLYLETDLPGDVIVHWGASRDETRKWEIPAAPHPPETTVFK 360
Query: 368 NKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVNLP 427
NKALRT LQ EG KG CGIFT+EEEF+GFLFVLKQNEN WLNYKGDDFYIPF SS +LP
Sbjct: 361 NKALRTLLQLKEGGKGSCGIFTIEEEFDGFLFVLKQNENPWLNYKGDDFYIPFPSSGHLP 420
Query: 428 DQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAKES 487
Q+RQ+E E+AGVS KAG+G E VS AYTDGIIKEIRN VTDI+SQRTQKK+T EA+ES
Sbjct: 421 GQKRQNEFEDAGVSRKAGDGYEEVSVTAYTDGIIKEIRNFVTDISSQRTQKKKTIEAQES 480
Query: 488 ILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWESH 547
ILQEIEKLAAEAYSIFRSA+PTFTE +E PKPVEPPV+ISSGTG+GFEILCQGFNWESH
Sbjct: 481 ILQEIEKLAAEAYSIFRSASPTFTEEIIEMPKPVEPPVQISSGTGSGFEILCQGFNWESH 540
Query: 548 KSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKDVV 607
KSGRWYM LKEKAAELSSLGFTVIWLPPPTESVSPEGYMP+DLYNL+SRYGNIDELKDVV
Sbjct: 541 KSGRWYMELKEKAAELSSLGFTVIWLPPPTESVSPEGYMPKDLYNLSSRYGNIDELKDVV 600
Query: 608 KTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 667
KTFHDVGI VLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG
Sbjct: 601 KTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSG 660
Query: 668 DNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 727
DNFHAAPNIDHSQDFVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF
Sbjct: 661 DNFHAAPNIDHSQDFVRNDIKEWLRWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYF 720
Query: 728 AVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 787
AVGEYWDSLSYTYGEMDH+QD HRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR
Sbjct: 721 AVGEYWDSLSYTYGEMDHDQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEYWR 780
Query: 788 LSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFF 847
LSDQKG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAY LTHPGTPSVFF
Sbjct: 781 LSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYTLTHPGTPSVFF 840
Query: 848 DHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEPPS 896
DHIFSHY S+IAAL+SLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPG++EPPS
Sbjct: 841 DHIFSHYKSDIAALISLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGYYEPPS 895
BLAST of Sgr029085 vs. TAIR 10
Match:
AT1G69830.1 (alpha-amylase-like 3 )
HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/857 (66.28%), Postives = 666/857 (77.71%), Query Frame = 0
Query: 66 APRASSTNAALLETFKSKDVLLKETFPIKRTEKVEGRIFVRLAQGKNQKQWELTVGCNLT 125
A RA+S++ A++ET +S DV+ KE FP++R EK EG+I+VRL + K +K WEL+VGC++
Sbjct: 63 AIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVK-EKNWELSVGCSIP 122
Query: 126 GKWILHWGVSLIGDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKI 185
GKWILHWGVS +GD+GSEWDQPP++M PPGSI IKDYAIETPLKK S GD+ EV I
Sbjct: 123 GKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSE---GDSFFEVAI 182
Query: 186 DLVPNKAISAINFVLKDEETGVWFQHKGRDFKVPFSDYHGDDGNIVGTKRSLGLWRAVVY 245
+L +++A+NFVLKDEETG W+QHKGRDFKVP D D+GN++G K+
Sbjct: 183 NLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKK---------- 242
Query: 246 HVGGMHFLNFFNIGALGQLSNLLVKAESTSKSQESSGESGDTKEEKKYLEGFYEELPIVK 305
GALGQLSN+ +K Q+ S D+ EE+K L+ FYEE+PI K
Sbjct: 243 -----------GFGALGQLSNIPLK-------QDKSSAETDSIEERKGLQEFYEEMPISK 302
Query: 306 EVAVDNSVSVSVR---------------------------KDETRKWEIPAAPHPPETTV 365
VA DNSVSV+ R K+ T+KWEIP+ P+P ET++
Sbjct: 303 RVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVCKNGTKKWEIPSEPYPEETSL 362
Query: 366 FKNKALRTPLQPIEGRKGCCGIFTLEEEFEGFLFVLKQNENSWLNYKGDDFYIPFSSSVN 425
FKNKALRT LQ + G G+F+L+ + EG FVLK NEN+WLNY+G+DFY+PF +S +
Sbjct: 363 FKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDFYVPFLTSSS 422
Query: 426 LPDQQRQSELEEAGVSGKAGEGSEGVSGVAYTDGIIKEIRNLVTDIASQRTQKKRTKEAK 485
P E E A VS + + VS +T II EIRNL DI+S + QK KE +
Sbjct: 423 SP-----VETEAAQVSKPKRKTDKEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQ 482
Query: 486 ESILQEIEKLAAEAYSIFRSAAPTFTEITLETPKPVEPPVKISSGTGTGFEILCQGFNWE 545
E+ILQEIEKLAAEAYSIFRS P F+E + + +P +KISSGTG+GFEILCQGFNWE
Sbjct: 483 ENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWE 542
Query: 546 SHKSGRWYMVLKEKAAELSSLGFTVIWLPPPTESVSPEGYMPRDLYNLNSRYGNIDELKD 605
S+KSGRWY+ L+EKA EL+SLGFTV+WLPPPTESVSPEGYMP+DLYNLNSRYG IDELKD
Sbjct: 543 SNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKD 602
Query: 606 VVKTFHDVGITVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKS 665
VK FH VGI VLGD VLNHRCAHFKNQNG+WN+FGGRLNWDDRAVV+DDPHFQGRGNKS
Sbjct: 603 TVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKS 662
Query: 666 SGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEP 725
SGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYVKDY+DAS+P
Sbjct: 663 SGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKP 722
Query: 726 YFAVGEYWDSLSYTYGEMDHNQDIHRQRIVDWINATNGTAGAFDVTTKGILHSALDRCEY 785
YFAVGEYWDSLSYTYGEMD+NQD HRQRIVDWINAT+G AGAFDVTTKGILH+AL +CEY
Sbjct: 723 YFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEY 782
Query: 786 WRLSDQKGQPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSV 845
WRLSD KG+PPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYILTHPGTP+V
Sbjct: 783 WRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAV 842
Query: 846 FFDHIFSHYYSEIAALMSLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAMKIGPGHFEP 896
FFDHIFS Y+SEIAAL+SLR R K++CRS V I+K+ERDVYAA IDEKVAMKIGPGH+EP
Sbjct: 843 FFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEP 882
BLAST of Sgr029085 vs. TAIR 10
Match:
AT1G14420.1 (Pectate lyase family protein )
HSP 1 Score: 541.6 bits (1394), Expect = 1.9e-153
Identity = 265/440 (60.23%), Postives = 313/440 (71.14%), Query Frame = 0
Query: 977 LSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAEVGQVLTGS---N 1036
L++ ++ VFD YW R +A K +++PHP VT+ N V + S N
Sbjct: 20 LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79
Query: 1037 STRRNL---------KKYTGPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGRKTIGGK 1096
TRR L K +G C A NPID CWRCD NWA+NRKKLADCVLGFGR+T GGK
Sbjct: 80 DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139
Query: 1097 LGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSDKTIDAR 1156
GP YVV D+SD+DL+NPKPGTLRHAV + GPLWIIF R+M I+L QEL++TSDKTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199
Query: 1157 GANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGDGISIFG 1216
GA V I GAG+TLQF+NNVIIH + + HIV G GG IRDS H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259
Query: 1217 SSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDAIMQVTV 1276
++NIWIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D + D MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319
Query: 1277 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNVYAK 1336
AFNHFG+ L QRMPRCR+G HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN AK
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379
Query: 1337 EVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMISYKPGT 1396
++TKREY+P WKSW W+SEGD +NGA+FV SG + P I KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439
Query: 1397 FVTRMTRFAGALPCFVGRPC 1400
V ++T AG L C +G C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459
BLAST of Sgr029085 vs. TAIR 10
Match:
AT3G01270.1 (Pectate lyase family protein )
HSP 1 Score: 530.4 bits (1365), Expect = 4.4e-150
Identity = 260/467 (55.67%), Postives = 323/467 (69.16%), Query Frame = 0
Query: 970 MVCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE----- 1029
+VC++ SL I D+YW +A EA+K A+ P P + D +
Sbjct: 9 LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68
Query: 1030 -------------------VGQVLTGSNSTRRNL------------KKYTGPCRATNPID 1089
+ + + +NSTRR+L K TGPC A+NPID
Sbjct: 69 TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128
Query: 1090 RCWRCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQ 1149
+CWRC P+WA RKKL CV GFG +T GGK G YVV D D+VNP+PGTLRHAVIQ
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188
Query: 1150 KGPLWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHH 1209
K PLWI+F +M+IRL+QEL++TSDKTIDARGANV IAYGAGIT+Q+++N+IIHGL +HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248
Query: 1210 IVVGTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAIT 1269
IV +GG IRDS++H G R +DGDGISIFG++NIW+DH+SMS C DGLIDAIMGSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308
Query: 1270 ISNCHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYT 1329
ISN HFTHHN+VML GA ++ D MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368
Query: 1330 HWLMYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNG 1389
HW +YAIGGSQ PTI+S GNRFIAPP+ + +EVTKR+Y+ E+ WK+W WRSE D+ MN
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428
Query: 1390 AFFVTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
A+F SG+ SR MI K G V+++T++AGAL C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475
BLAST of Sgr029085 vs. TAIR 10
Match:
AT5G15110.1 (Pectate lyase family protein )
HSP 1 Score: 524.6 bits (1350), Expect = 2.4e-148
Identity = 258/464 (55.60%), Postives = 320/464 (68.97%), Query Frame = 0
Query: 973 LLLTLSLAVAI--ANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLNAE------ 1032
L+ T +AV I +I D+YW RA EA++ A+ P + D +
Sbjct: 9 LMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNST 68
Query: 1033 --------------------VGQVLTGSNSTRRNLK--------KYTGPCRATNPIDRCW 1092
+ + + +NSTRR+L+ K GPC A+NPID+CW
Sbjct: 69 DVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCW 128
Query: 1093 RCDPNWASNRKKLADCVLGFGRKTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGP 1152
RC +WA RKKL CV GFG +T GGK G YVV + D D+VNPKPGTLRHAVIQK P
Sbjct: 129 RCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEP 188
Query: 1153 LWIIFDRNMAIRLTQELIMTSDKTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVV 1212
LWIIF +M+IRL QEL++ S KTIDARGANV +A+GAGIT+QF+ NV+IHGL IHHI
Sbjct: 189 LWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISE 248
Query: 1213 GTGGTIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISN 1272
+GG IRDSVDH G+RT +DGDG+SI+GSSNIW+DH+SMS C DGLIDAI+GST ITISN
Sbjct: 249 SSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISN 308
Query: 1273 CHFTHHNEVMLFGASDSYSGDAIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 1332
HFTHHN+VML GA ++ D MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW
Sbjct: 309 SHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWE 368
Query: 1333 MYAIGGSQHPTIISQGNRFIAPPN-VYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFF 1392
+YAIGGSQ PTI+S GNRFIAPP+ + +EVTKR+Y+ E+ WK W WRS+ D+ MNGA+F
Sbjct: 369 LYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYF 428
Query: 1393 VTSGDQRKSRPFSRLDMISYKPGTFVTRMTRFAGALPCFVGRPC 1400
SG+ + +R MI K G V+++T++AGAL C VGR C
Sbjct: 429 RQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472
BLAST of Sgr029085 vs. TAIR 10
Match:
AT2G02720.1 (Pectate lyase family protein )
HSP 1 Score: 499.6 bits (1285), Expect = 8.2e-141
Identity = 257/446 (57.62%), Postives = 310/446 (69.51%), Query Frame = 0
Query: 971 VCLLLTLSLAVAIANADIGVFDDYWKARAIEAQKAAATAFEPHPEAVTDSLN--AEVGQV 1030
V + L+L++ V A I +D+YW R A + +++P+PE VTD N A +
Sbjct: 10 VFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAME 69
Query: 1031 LTG-SNSTRRNL------KKYT---GPCRATNPIDRCWRCDPNWASNRKKLADCVLGFGR 1090
TG N TRR+L KK T G + N ID+CWR D NW NRKKLADCVLGFGR
Sbjct: 70 TTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGR 129
Query: 1091 KTIGGKLGPYYVVNDSSDSDLVNPKPGTLRHAVIQKGPLWIIFDRNMAIRLTQELIMTSD 1150
KT GGK GP YVV D SD+DL+ PKPGT+RHAV + PLWIIF R+M I+L QELI+T+D
Sbjct: 130 KTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITND 189
Query: 1151 KTIDARGANVQIAYGAGITLQFINNVIIHGLRIHHIVVGTGGTIRDSVDHVGLRTMSDGD 1210
KTID RGA + I GAG+TLQF+ NVIIH + I I G GG I DS H GLRT+SDGD
Sbjct: 190 KTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGD 249
Query: 1211 GISIFGSSNIWIDHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSGDA 1270
GI+IFG++N+WIDHVSM++C DG+IDAIMGSTAITISN HFT H+EVMLFG ++ D
Sbjct: 250 GINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDK 309
Query: 1271 IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 1330
MQ+TVAFNHFG+ L QRMPR R+G HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAP
Sbjct: 310 KMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAP 369
Query: 1331 PNVYAKEVTKREYSPENVWKSWTWRSEGDLMMNGAFFVTSG-----DQRKSRPFSRLDMI 1390
P +K+VTKREY+P WKSW W+SE D +NGA+FV SG P R I
Sbjct: 370 PIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAI 429
Query: 1391 SYKPGTFVTRMTRFAGALPCFVGRPC 1400
+PGT V R+T+ AG L C G+ C
Sbjct: 430 RPQPGTKVRRLTKDAGTLGCKPGKSC 455
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
TYK15529.1 | 0.0e+00 | 81.54 | alpha-amylase 3 [Cucumis melo var. makuwa] | [more] |
RXH69880.1 | 0.0e+00 | 61.67 | hypothetical protein DVH24_007136 [Malus domestica] | [more] |
RXH79597.1 | 0.0e+00 | 60.01 | hypothetical protein DVH24_040744 [Malus domestica] | [more] |
RZC60748.1 | 0.0e+00 | 58.78 | hypothetical protein C5167_022499 [Papaver somniferum] | [more] |
XP_038891960.1 | 0.0e+00 | 84.59 | alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
Q94A41 | 0.0e+00 | 66.28 | Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1 | [more] |
P15722 | 6.5e-167 | 65.98 | Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1 | [more] |
P40973 | 2.6e-155 | 63.66 | Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1 | [more] |
Q9M9S2 | 2.7e-152 | 60.23 | Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1 | [more] |
Q9SRH4 | 6.1e-149 | 55.67 | Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3CZB5 | 0.0e+00 | 81.54 | Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 P... | [more] |
A0A498HGM8 | 0.0e+00 | 61.67 | Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_007136 PE=3 SV=1 | [more] |
A0A498IC60 | 0.0e+00 | 60.01 | Pectate lyase OS=Malus domestica OX=3750 GN=DVH24_040744 PE=3 SV=1 | [more] |
A0A4Y7JLT3 | 0.0e+00 | 58.78 | Pectate lyase OS=Papaver somniferum OX=3469 GN=C5167_022499 PE=3 SV=1 | [more] |
A0A6J1DCP4 | 0.0e+00 | 85.57 | 1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110191... | [more] |