Sgr028994 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr028994
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationtig00153210: 2398162 .. 2401212 (-)
RNA-Seq ExpressionSgr028994
SyntenySgr028994
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTATAGAAATGTTGCAGCACCCTTGACAAAACTACCTTCAGTGGCTCACTACATAGAGCCACTCTTTGAGACCTTCTTCTGTCCATTGACAAAGGATATTATGGATGATCCAGTTAGCCTAGAAACGGGAGTGACGTATGAAAGACAAGCAATAGTTGAGTGGTTTGAAGAGTTTGAAGAATCTGAGGAAATTTTCTGCCCAGTCACAGGGCAAAAGCTGGTGTCTAAAGCTTTTAATTCCAACAGAGCTCTGAAGTCACTATAGACAAATGGAAGGAACGCAATGAGATAGCAAGAATCAAAGTTGCCCGGGCTGCTCTGTCTTTAGCTAGTTCAGATGATATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCTGCAAAGGGAAGCAGTGCAACATAGAACGAATCTTCAATTTTGACATGTTACCTCTGCTCGTTAAGTTTCTGGACTACAGAGACAAAGATGTTAGATATGAAGTTCTAGAGCTATTGCAGGAAATGGCAGAAATCAATGAAGACAACAAGGTTTAAACTCACTCTACTAGTCTTATTATTATACAAAATTGATCACTGTCATGCCTCTGCAGTTCCTTCCTCCCCCCCATCCCCCCTCCCCAAATAAATAAAAGAAAAAGGAAGAATGAAAAGAAATAAATAATTAAAGAGTAGAGAAAGAAAATAGCTTTTGCAATTTATTCTAAGGGTCCATGCTTCTAAAGACTTCCGTTTTTAAATGAATAAACTGAAATCAAGTGAAATATAAACATCATGCCTGCAAACATGCTAGAGACTTAGAATCCACTGAAAGGTCAAAACTATAAAAGAAAAAAACTAAAATTGATACTAGTATAATCTAGTTAGTAGTCATCTAAAACGATTACAATTCAGCTATTGTTGACCAAAAACATGGAAAAAAGAAGAAAAGAACCCAAACTTTTATCTCTCTCCAATCCTCTAATCTAATAATAATAGTAATAATAATAATAATTCTCTCCCTATGCTTTCTTGCAAGATAAATCTTTAACCTTGCAACAGCCATGAGTTTTCAATGATCATTACCTATCTTTTACTCTATCATAGTGAACATCCAACTTCTGCAACCAAATATGATAGATTAGAACTATATAGGCTAATTTGAACGGTATATTTCCAAAATACTACCCCTTAGTATTAAATAACATAGCTGAACTCTATTAGGCCAATCTCTTGATTAGAAACTGTACAGAAGACTCTTCCAAAATTCTCCCACAAGCCACAGTGAAGTAGTTTCTCTTTCTTTAACTTATTTTATGGTGTAAAATCTTAGTTATTACTGATTAACATGTAACAAAATAAGTTCCCCTCCCCCCTAGTTTTACAATATAAAAGACCACATTTTTCTTGACAGACTGATAACATTAGTCAAATCAGGATTTGGAAACAGTTCTACCACATCTATATGGTGACCAGTTAAGCTACTGGTACCTTTATTAGTAATCAGTCCCTGTTCAGTAGGGTAGAATTTAATGAAATGAACGCCCACAAGTCCTGGTTCTGCTCTCTTGTGCATGCACATTCATAAATACCTAAAAGTACAGATAACACAATTAGTTAGGGAAAGTAGAATACCAATCTTCAACAATCTTGCAATTCAAAATAAAATAAAATAAAACAAACAGAAATGCAGAAGTATGACACATCATTTTCTATTTCTTATTATGCAGAAAATGATCTGTAACCAATTGGACTTGTCAAGAATGATCATTCTACTATCAAGTAGTCACCGGTCCATCCGGAATACATCTTTGCTTCTGTTGCTTGAGCTTTCAAGATCCCAGTCATTGTCTGATCAAATTGGTTCAGTAACTGGGGGAATTTCGATGCTGATCACCATGAAGTACGATAGGTCTGATGAATTTGCTTCAGAAAGGGCAGATGAAACCTTGAGAAACTTAGAGAGATCTCCAACTAATATCAAGCTTATGGCAGAACATGGACTCATGAAACCCCTCTTACGAGATCTTACTGAAGGTGAGTGTAAAATTCTCACGCTCCCAACATAAGCATCCTTCACATGGTTTGAAGAATAATATTAATAGAATAACTGAAGTGCTGAACAATACAAAAATATCTGGATGAATATATGTTGGGAATCAGTAAATGTCCACAACAGGTAACATGTAAATTGATTGTTAAGTGAATGATCAAACTCACCTCAGAATTTAATATTGCTTTTGTTGCATTTAAGCTTGAAGCAAGTAAATAGGGATGATTTCCAATCGCCATATACCAATTAAGTAATTTAAATACAGGTAGTGAGTGGACGAGAATAGAAATGGCAAGCTATCTTGGGGAGATGGTTATTGAACATGATTGCATGACGCATGTAGCTGAGAGAGCCTCTCCAGCTCTTGTTCAGATGGTGCACGAAGGAGATACACTAGTCCGAAAGGCAGCATTTAAAGCTCTATTGCAAATTTCATCTCACAAGCCAAATGGTAAAATACTTGCAAATGCTGGAACTGTACAAGTCATGGTTGAAGAGATGTTCACTCGTACCATCTGTGATGAACTTAATGACCCAAAAAGAGAAGCAGCTCGAATACTGGCAAATATATGTGAGTCCTGTCTTGATCTTGAGACAGTTCGAGTTAACACTCATGGCTCCACTATGAGTTCAGATTATGTAGTCCACAACATTATCGACTTGCTCAAGAACTCAACCCCAGATGAATTTAGCATGAGCCTCATCAGGATACTATTATGCTTGACAAAATCTCCAAAACCAATGGCCACCATTGTTTCAGGTGTAAAGACACTGAAGCAGGTGATACCATCATTGAGTTCATCAATAGTCCAGATGAGGAACTTGGAGTGCAGCAATAAAACTGCTTATATCACTGTCCCCTTACATGGGCTACACAATGGCAGAAAGGCTCTGCAAAACCAGTGGCCAAGTGGAGAACCTTATCAGGAGCATTACCTGGACAAACCAAATCACAGAGAAGCAGGCACTTTCAGCAACATTCTTAGCAAAACTACCTCACCAGAGCCTAACTCTTAA

mRNA sequence

ATGAAGTATAGAAATGTTGCAGCACCCTTGACAAAACTACCTTCAGTGGCTCACTACATAGAGCCACTCTTTGAGACCTTCTTCTGTCCATTGACAAAGGATATTATGGATGATCCAGTTAGCCTAGAAACGGGAGTGACGTATGAAAGACAAGCAATAGTTGAGTGGTTTGAAGAGTTTGAAGAATCTGAGGAAATTTTCTGCCCAAGCTCTGAAGTCACTATAGACAAATGGAAGGAACGCAATGAGATAGCAAGAATCAAAGTTGCCCGGGCTGCTCTGTCTTTAGCTAGTTCAGATGATATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCTGCAAAGGGAAGCAGTGCAACATAGAACGAATCTTCAATTTTGACATGTTACCTCTGCTCGTTAAGTTTCTGGACTACAGAGACAAAGATGTTAGATATGAAGTTCTAGAGCTATTGCAGGAAATGGCAGAAATCAATGAAGACAACAAGAAAATGATCTGTAACCAATTGGACTTGTCAAGAATGATCATTCTACTATCAAGTAGTCACCGGTCCATCCGGAATACATCTTTGCTTCTGTTGCTTGAGCTTTCAAGATCCCAGTCATTGTCTGATCAAATTGGTTCAGTAACTGGGGGAATTTCGATGCTGATCACCATGAAGTACGATAGGTCTGATGAATTTGCTTCAGAAAGGGCAGATGAAACCTTGAGAAACTTAGAGAGATCTCCAACTAATATCAAGCTTATGGCAGAACATGGACTCATGAAACCCCTCTTACGAGATCTTACTGAAGGTAGTGAGTGGACGAGAATAGAAATGGCAAGCTATCTTGGGGAGATGGTTATTGAACATGATTGCATGACGCATGTAGCTGAGAGAGCCTCTCCAGCTCTTGTTCAGATGGTGCACGAAGGAGATACACTAGTCCGAAAGGCAGCATTTAAAGCTCTATTGCAAATTTCATCTCACAAGCCAAATGGTAAAATACTTGCAAATGCTGGAACTGTACAAGTCATGGTTGAAGAGATGTTCACTCGTACCATCTGTGATGAACTTAATGACCCAAAAAGAGAAGCAGCTCGAATACTGGCAAATATATGTGAGTCCTGTCTTGATCTTGAGACAGTTCGAGTTAACACTCATGGCTCCACTATGAGTTCAGATTATGTAGTCCACAACATTATCGACTTGCTCAAGAACTCAACCCCAGATGAATTTAGCATGAGCCTCATCAGGATACTATTATGCTTGACAAAATCTCCAAAACCAATGGCCACCATTGTTTCAGGTGTAAAGACACTGAAGCAGGTGATACCATCATTGAGTTCATCAATAGTCCAGATGAGGAACTTGGAGTGCAGCAATAAAACTGCTTATATCACTGTCCCCTTACATGGGCTACACAATGGCAGAAAGGCTCTGCAAAACCAGTGGCCAAGTGGAGAACCTTATCAGGAGCATTACCTGGACAAACCAAATCACAGAGAAGCAGGCACTTTCAGCAACATTCTTAGCAAAACTACCTCACCAGAGCCTAACTCTTAA

Coding sequence (CDS)

ATGAAGTATAGAAATGTTGCAGCACCCTTGACAAAACTACCTTCAGTGGCTCACTACATAGAGCCACTCTTTGAGACCTTCTTCTGTCCATTGACAAAGGATATTATGGATGATCCAGTTAGCCTAGAAACGGGAGTGACGTATGAAAGACAAGCAATAGTTGAGTGGTTTGAAGAGTTTGAAGAATCTGAGGAAATTTTCTGCCCAAGCTCTGAAGTCACTATAGACAAATGGAAGGAACGCAATGAGATAGCAAGAATCAAAGTTGCCCGGGCTGCTCTGTCTTTAGCTAGTTCAGATGATATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCTGCAAAGGGAAGCAGTGCAACATAGAACGAATCTTCAATTTTGACATGTTACCTCTGCTCGTTAAGTTTCTGGACTACAGAGACAAAGATGTTAGATATGAAGTTCTAGAGCTATTGCAGGAAATGGCAGAAATCAATGAAGACAACAAGAAAATGATCTGTAACCAATTGGACTTGTCAAGAATGATCATTCTACTATCAAGTAGTCACCGGTCCATCCGGAATACATCTTTGCTTCTGTTGCTTGAGCTTTCAAGATCCCAGTCATTGTCTGATCAAATTGGTTCAGTAACTGGGGGAATTTCGATGCTGATCACCATGAAGTACGATAGGTCTGATGAATTTGCTTCAGAAAGGGCAGATGAAACCTTGAGAAACTTAGAGAGATCTCCAACTAATATCAAGCTTATGGCAGAACATGGACTCATGAAACCCCTCTTACGAGATCTTACTGAAGGTAGTGAGTGGACGAGAATAGAAATGGCAAGCTATCTTGGGGAGATGGTTATTGAACATGATTGCATGACGCATGTAGCTGAGAGAGCCTCTCCAGCTCTTGTTCAGATGGTGCACGAAGGAGATACACTAGTCCGAAAGGCAGCATTTAAAGCTCTATTGCAAATTTCATCTCACAAGCCAAATGGTAAAATACTTGCAAATGCTGGAACTGTACAAGTCATGGTTGAAGAGATGTTCACTCGTACCATCTGTGATGAACTTAATGACCCAAAAAGAGAAGCAGCTCGAATACTGGCAAATATATGTGAGTCCTGTCTTGATCTTGAGACAGTTCGAGTTAACACTCATGGCTCCACTATGAGTTCAGATTATGTAGTCCACAACATTATCGACTTGCTCAAGAACTCAACCCCAGATGAATTTAGCATGAGCCTCATCAGGATACTATTATGCTTGACAAAATCTCCAAAACCAATGGCCACCATTGTTTCAGGTGTAAAGACACTGAAGCAGGTGATACCATCATTGAGTTCATCAATAGTCCAGATGAGGAACTTGGAGTGCAGCAATAAAACTGCTTATATCACTGTCCCCTTACATGGGCTACACAATGGCAGAAAGGCTCTGCAAAACCAGTGGCCAAGTGGAGAACCTTATCAGGAGCATTACCTGGACAAACCAAATCACAGAGAAGCAGGCACTTTCAGCAACATTCTTAGCAAAACTACCTCACCAGAGCCTAACTCTTAA

Protein sequence

MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEESEEIFCPSSEVTIDKWKERNEIARIKVARAALSLASSDDMVLEAIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPDEFSMSLIRILLCLTKSPKPMATIVSGVKTLKQVIPSLSSSIVQMRNLECSNKTAYITVPLHGLHNGRKALQNQWPSGEPYQEHYLDKPNHREAGTFSNILSKTTSPEPNS
Homology
BLAST of Sgr028994 vs. NCBI nr
Match: XP_022149110.1 (putative U-box domain-containing protein 42 [Momordica charantia])

HSP 1 Score: 714.9 bits (1844), Expect = 4.9e-202
Identity = 371/448 (82.81%), Postives = 406/448 (90.62%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           MKYRNV   LTKLPS+AHYIEPLFETFFCPLTKDIMDDPVSLETG TYERQAIV+WFEE+
Sbjct: 205 MKYRNVTESLTKLPSMAHYIEPLFETFFCPLTKDIMDDPVSLETGATYERQAIVDWFEEY 264

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEEIFCP               + + TIDKWKERNEIARIKVARAALSLASSD+MVLE
Sbjct: 265 EESEEIFCPVTGQKLVSKAFNSNRALKSTIDKWKERNEIARIKVARAALSLASSDEMVLE 324

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AI+DL SICKGKQCN+ERIFNFDMLPLLVKFL+Y+D+DVRY VLELLQ+MAEI+ED KKM
Sbjct: 325 AIEDLLSICKGKQCNVERIFNFDMLPLLVKFLEYKDRDVRYGVLELLQQMAEIDEDKKKM 384

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           I NQLD+ RMI LLSSSH+ IRNTSLLL+LELSRSQSLSDQIGSVTG I MLITMKY+RS
Sbjct: 385 IYNQLDMLRMIKLLSSSHQFIRNTSLLLVLELSRSQSLSDQIGSVTGAIPMLITMKYNRS 444

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFAS +ADETLRNLERSPTNIKLMAEHGL++PLLR LTEGSEW RIEMASYLGEMVIEH
Sbjct: 445 DEFASRKADETLRNLERSPTNIKLMAEHGLLEPLLRYLTEGSEWMRIEMASYLGEMVIEH 504

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           +CM HVAE ASPALV+MVHEGD  +RKAAFKALLQISSHKPNGKILA AGTVQVMVEEMF
Sbjct: 505 NCMAHVAETASPALVKMVHEGDAFIRKAAFKALLQISSHKPNGKILAKAGTVQVMVEEMF 564

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK+EAARILANICES L+ E ++VN+HGSTMSS+YVV+NIID+L+NSTPD
Sbjct: 565 TRTICDELNDPKKEAARILANICESNLNPEILQVNSHGSTMSSEYVVYNIIDVLENSTPD 624

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           EFS+SL+RILLCLTKSPKP+ TIVSGVK
Sbjct: 625 EFSLSLVRILLCLTKSPKPIDTIVSGVK 652

BLAST of Sgr028994 vs. NCBI nr
Match: XP_023542844.1 (putative U-box domain-containing protein 42 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.9 bits (1766), Expect = 5.5e-193
Identity = 362/449 (80.62%), Postives = 394/449 (87.75%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV    TK PS A+YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIVEWFEEF
Sbjct: 208 VKYRNVTEVSTKRPSTANYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVEWFEEF 267

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD+MVLE
Sbjct: 268 EESEEVFCPVTGQKLVSKAVNSNRALKSTIDKWKERNEIATIKVARAALSLASSDEMVLE 327

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AIKDLSSI KGKQ NIERIFNFDMLPLLVK L+YR+KDVRY +LELL +MAEINEDNKK+
Sbjct: 328 AIKDLSSISKGKQFNIERIFNFDMLPLLVKCLEYRNKDVRYALLELLHQMAEINEDNKKI 387

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICNQLD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 388 ICNQLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKDDRS 447

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGE+VI  
Sbjct: 448 DEFASEKADETLRNLETSPTNIKLMAEYGLMEPLVRHLTEGNEWMRIEMASYLGEIVIIQ 507

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 508 DCMAYVAEKASPALVDMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 567

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TR ICDELNDPK EAA ILANICES LDLET++VN HGSTMSSDY+V+NIIDLL  STPD
Sbjct: 568 TRIICDELNDPKTEAAEILANICESSLDLETLQVNAHGSTMSSDYIVYNIIDLLDISTPD 627

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLCLTKSPK M TIVSGVK+
Sbjct: 628 EFSKSLIRILLCLTKSPKAMDTIVSGVKS 656

BLAST of Sgr028994 vs. NCBI nr
Match: XP_022967965.1 (putative U-box domain-containing protein 42 [Cucurbita maxima] >XP_022967966.1 putative U-box domain-containing protein 42 [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 7.9e-192
Identity = 361/449 (80.40%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV A  TK PS A+YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIV+WFEEF
Sbjct: 208 VKYRNVTAVSTKRPSTANYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVQWFEEF 267

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD M+LE
Sbjct: 268 EESEEVFCPVTGQKLVSKAFNSNRALKSTIDKWKERNEIATIKVARAALSLASSDKMMLE 327

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AIKDLSSI KGKQ NIERIF+FDMLPLLV+ L+YR+KDVRY VLELL +MAEINEDNKK 
Sbjct: 328 AIKDLSSISKGKQFNIERIFDFDMLPLLVECLEYRNKDVRYAVLELLHQMAEINEDNKKT 387

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICNQLD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 388 ICNQLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKNDRS 447

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGEMVI  
Sbjct: 448 DEFASEKADETLRNLEISPTNIKLMAEYGLMEPLVRHLTEGNEWMRIEMASYLGEMVIRQ 507

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 508 DCMAYVAEKASPALVGMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 567

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK EAA ILANIC+S LDLET++VN HGSTMSSDY+V NIIDLL  STPD
Sbjct: 568 TRTICDELNDPKTEAAEILANICDSSLDLETLQVNAHGSTMSSDYIVCNIIDLLDISTPD 627

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLCLTKSPK M TIVSGVK+
Sbjct: 628 EFSKSLIRILLCLTKSPKTMDTIVSGVKS 656

BLAST of Sgr028994 vs. NCBI nr
Match: XP_022944907.1 (putative U-box domain-containing protein 42 [Cucurbita moschata])

HSP 1 Score: 678.3 bits (1749), Expect = 5.1e-191
Identity = 359/449 (79.96%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV    TK PS A+YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIVEWFEEF
Sbjct: 208 VKYRNVTEVSTKRPSTANYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVEWFEEF 267

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD MVLE
Sbjct: 268 EESEEVFCPVTGQKLVSKAVNSNRALKSTIDKWKERNEIATIKVARAALSLASSDAMVLE 327

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AI+DLSSI KGKQ NIERIF+F+MLPLLV+ L+YR+KDVRY VLELL +MAEINEDNKK+
Sbjct: 328 AIEDLSSISKGKQFNIERIFDFEMLPLLVECLEYRNKDVRYAVLELLHQMAEINEDNKKI 387

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICNQLD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 388 ICNQLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKDDRS 447

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGE+VI  
Sbjct: 448 DEFASEKADETLRNLETSPTNIKLMAEYGLMEPLVRYLTEGNEWMRIEMASYLGEIVIRQ 507

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 508 DCMAYVAEKASPALVDMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 567

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK EAA ILANICES LDLET++VN HGSTMSSDY+V+NIIDLL  STPD
Sbjct: 568 TRTICDELNDPKTEAAEILANICESSLDLETLQVNAHGSTMSSDYIVYNIIDLLDISTPD 627

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLCLTKSPK M TIV GVK+
Sbjct: 628 EFSKSLIRILLCLTKSPKTMDTIVLGVKS 656

BLAST of Sgr028994 vs. NCBI nr
Match: KAG6574024.1 (putative U-box domain-containing protein 42, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013084.1 putative U-box domain-containing protein 42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675.2 bits (1741), Expect = 4.3e-190
Identity = 357/449 (79.51%), Postives = 392/449 (87.31%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV    TK  S  +YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIVEWFEEF
Sbjct: 196 VKYRNVTEVSTKRSSTTNYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVEWFEEF 255

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD+MVLE
Sbjct: 256 EESEEVFCPVTGQKLVSKAVNSNRALKSTIDKWKERNEIATIKVARAALSLASSDEMVLE 315

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AIKDLSSI KGKQ NIERIF+F+MLPLLV+ L+YR+KDVRY VLELL +MAEINEDNKK+
Sbjct: 316 AIKDLSSISKGKQFNIERIFDFEMLPLLVECLEYRNKDVRYAVLELLHQMAEINEDNKKI 375

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICN+LD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 376 ICNKLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKDDRS 435

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGE+VI  
Sbjct: 436 DEFASEKADETLRNLETSPTNIKLMAEYGLMEPLVRYLTEGNEWMRIEMASYLGEIVIRQ 495

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 496 DCMAYVAEKASPALVDMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 555

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK EAA ILANICES LDLET++VN HGSTMSSDY+V+NIIDLL  STPD
Sbjct: 556 TRTICDELNDPKTEAAEILANICESSLDLETLQVNAHGSTMSSDYIVYNIIDLLDISTPD 615

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLC TKSPK M TIVSGVK+
Sbjct: 616 EFSKSLIRILLCWTKSPKTMDTIVSGVKS 644

BLAST of Sgr028994 vs. ExPASy Swiss-Prot
Match: Q9CAA7 (Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=PUB42 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.7e-115
Identity = 226/448 (50.45%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           KY N++  L+ LP V  ++EP ++ F CPLTK+IM+DPV+ ETGVT ERQA++EWF+ F 
Sbjct: 225 KYGNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFG 284

Query: 62  ESEEIFCPSS--------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEAI 121
            S+EI CP +              +  I +WK RNE ARIKVA AALSL  S+ MV++A+
Sbjct: 285 NSDEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 344

Query: 122 KDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMA-EINEDNKKMI 181
           +DL   C+GK+ N  ++    ++ LL ++L YR KDVR+E+L+ L+ +A E  +D K+MI
Sbjct: 345 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 404

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              + +S +I LL SSH+ +R+ +  LLLELS+SQ   ++IG+  G I ML+T KY+R  
Sbjct: 405 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 464

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASE +D+ LRNLE+ P NIK MAE GL++PLL  L EGSE T++ MA+YL E+ I H
Sbjct: 465 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGH 524

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           +  T+VAE+A PAL+ +V   +   R+AAFKAL  IS + PN KIL   G +++MVEEMF
Sbjct: 525 EKKTYVAEKACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 584

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
           T+ +  +L + + EAA ILANI ES L+ ET  VNTHG T+ SDY V+NII +LKNS+PD
Sbjct: 585 TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 644

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           + ++ LIRILL L+KSP+ MATIVS +K
Sbjct: 645 DLNIDLIRILLSLSKSPRAMATIVSVIK 672

BLAST of Sgr028994 vs. ExPASy Swiss-Prot
Match: Q9SFX2 (U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.8e-58
Identity = 148/468 (31.62%), Postives = 261/468 (55.77%), Query Frame = 0

Query: 20  IEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEES-EEIFCP--SSEV--- 79
           I+ ++E F CPLTK +M +PV+LE G T+ER+AI +WF+E  E+ + + CP  S E+   
Sbjct: 22  IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKWFQECRENGQPLSCPITSKELSIT 81

Query: 80  ----------TIDKWKERNEIARIKVARAALSLASSDDMVLEAIKDLSSICKGKQCNIER 139
                     TI++W+ RN+  ++ +AR +L L +++  +L A+K++  IC+  +   +R
Sbjct: 82  DLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVREICRNIRKIRQR 141

Query: 140 IFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMIILLSSSH 199
           + N  ++ L+   L     +VR + L+ LQ + E +E++K ++     +  ++  LS   
Sbjct: 142 VCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDTVRTIVKFLSQEP 201

Query: 200 RSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFAS-ERADETLRNLER 259
              R  ++ +L ELS+S++L ++IGS+ G I +L+ +   +S+  ++ E+AD+TL NLER
Sbjct: 202 SKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTVEKADKTLTNLER 261

Query: 260 SPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERASPALVQM 319
           S  N++ MA +G ++PLL  L EGS  T++ MA YLG + + +D    VA+    +L+ +
Sbjct: 262 SEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVIVAQTVGSSLIDL 321

Query: 320 VHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELNDPKREAAR 379
           +   D   R+AA  AL  ISS + + K+L N G +  +++++F           K  +A 
Sbjct: 322 MRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGPNQLPIRLKEVSAT 381

Query: 380 ILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPDEFSMSLIRILLCLTKSP 439
           ILANI     D + V V  H  T+ S+ +V N++ L  N+ P E    L+ +L+ LT  P
Sbjct: 382 ILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGP-EIQGKLLAVLVGLTSCP 441

Query: 440 KPMATIVSGVKTLKQVIPSLSSSIVQMRNLECSNKTAYITVPLHGLHN 471
             +  +VS ++    +I     S+VQ   +  ++     ++ L  LHN
Sbjct: 442 NSVINVVSAIRNSAAII-----SLVQFVEIHENDDLRLASIKL--LHN 481

BLAST of Sgr028994 vs. ExPASy Swiss-Prot
Match: Q9LM76 (U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.9e-56
Identity = 146/468 (31.20%), Postives = 261/468 (55.77%), Query Frame = 0

Query: 20  IEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEES-EEIFCP-------SS 79
           ++ ++E F CPLTK++M DPV+LE G T+ER+AI +WF+E  +S     CP       S+
Sbjct: 20  VDHIYEAFICPLTKEVMHDPVTLENGRTFEREAIEKWFKECRDSGRPPSCPLTSQELTST 79

Query: 80  EV--------TIDKWKERNEIARIKVARAALSLASSDDMVLEAIKDLSSICKGKQCNIER 139
           +V        TI++W+ RN+ A++ +AR +L L +++  +L+A+  +  IC+  + N   
Sbjct: 80  DVSASIALRNTIEEWRSRNDAAKLDIARQSLFLGNAETDILQALMHVRQICRTIRSNRHG 139

Query: 140 IFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMIILLSSSH 199
           + N  ++ +++  L      VRY+ L+ LQ + E ++++K ++     +  ++  LS   
Sbjct: 140 VRNSQLIHMIIDMLKSTSHRVRYKALQTLQVVVEGDDESKAIVAEGDTVRTLVKFLSHEP 199

Query: 200 RSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFA-SERADETLRNLER 259
              R  ++ LL ELS+S++L ++IGS+ G + +L+ +    S+  +  E+AD TL N+ER
Sbjct: 200 SKGREAAVSLLFELSKSEALCEKIGSIHGALILLVGLTSSNSENVSIVEKADRTLENMER 259

Query: 260 SPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERASPALVQM 319
           S   ++ MA +G ++PLL  L EGS  T++ MAS+LGE+ + +D    VA+    +LV +
Sbjct: 260 SEEIVRQMASYGRLQPLLGKLLEGSPETKLSMASFLGELPLNNDVKVLVAQTVGSSLVDL 319

Query: 320 VHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELNDPKREAAR 379
           +  GD   R+AA KAL +ISS + + K+L + G +  +++++F     +     K  +A 
Sbjct: 320 MRSGDMPQREAALKALNKISSFEGSAKVLISKGILPPLIKDLFYVGPNNLPIRLKEVSAT 379

Query: 380 ILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPDEFSMSLIRILLCLTKSP 439
           ILANI     D +        +T+ S+  V N++ L+ N+ P      L+ +L+ LT  P
Sbjct: 380 ILANIVNIGYDFDK-------ATLVSENRVENLLHLISNTGP-AIQCKLLEVLVGLTSCP 439

Query: 440 KPMATIVSGVKTLKQVIPSLSSSIVQMRNLECSNKTAYITVPLHGLHN 471
           K +  +V  +KT   +I     S+VQ   +  ++     ++ L  LHN
Sbjct: 440 KTVPKVVYAIKTSGAII-----SLVQFIEVRENDDLRLASIKL--LHN 472

BLAST of Sgr028994 vs. ExPASy Swiss-Prot
Match: Q10FT0 (U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.5e-47
Identity = 145/452 (32.08%), Postives = 237/452 (52.43%), Query Frame = 0

Query: 21  EPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEES-EEIFCP-------SSE 80
           E  FE F CPLTK +M DPV++ETG T+ER+AI++WF E  ++     CP        +E
Sbjct: 12  EGAFEAFVCPLTKQVMRDPVTIETGQTFEREAILKWFRECRDNGRRPTCPLTQRELRDTE 71

Query: 81  VT--------IDKWKERNEIARIKVARAAL--------SLASSDDMVLEAIKDLSSICKG 140
           V+        I +W+ RNE   +  A A+L             ++  L A+  +S IC+ 
Sbjct: 72  VSPSVALRSVIHEWRARNEEKDLDRACASLVGGFAGHAGDEEEEESALRALVHVSQICQR 131

Query: 141 KQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMI 200
              + + +    +L  + + L    + +R + L++L+ + E N+DNK+ +     +  +I
Sbjct: 132 SAASKDLVRRRGVLRAVAEMLKSGSRRLRLKSLQVLRVLVEDNDDNKEELGKGDTIRTII 191

Query: 201 ILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDE-FASERADE 260
             LS+ H   R  ++ LL ELS  +   ++IG+V G I +L+ M   +S+   A ++A+ 
Sbjct: 192 KFLSNEHVQERELAVSLLHELSGHEPTCERIGAVYGAILLLVGMGSSKSESAVAVDKAES 251

Query: 261 TLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERA 320
           TLRNL+R   N+K MA++G ++PLL  L  G   TR+ MA YLGE+ + +D    VAE+A
Sbjct: 252 TLRNLDRFDANVKQMADNGRLQPLLTRLLRGEPDTRVAMADYLGELALANDDKAAVAEQA 311

Query: 321 SPALVQMVHEGDTLVRKAAFKALLQISSHKPNGK-ILANAGTVQVMVEEMFTRTICDELN 380
            P LV M+  G T  ++A  KAL +ISS + + K +L  AG +  +V ++   T    + 
Sbjct: 312 GPLLVGMLRTGATPAKEATLKALREISSSEASAKLLLQRAGVLPPLVNDVLFSTGHLPMK 371

Query: 381 DPKREAARILANICESCLDLETVRVNT------------HGSTMSSDYVVHNIIDLLKNS 435
             K  AA ILAN+  S  D  ++ ++                T+ S+ VVH+ + L+ N+
Sbjct: 372 -LKELAATILANLVASGADFRSIPLDDDEDDDGGGGGRGRRRTLLSEDVVHSQLHLISNT 431

BLAST of Sgr028994 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 2.0e-12
Identity = 91/376 (24.20%), Postives = 163/376 (43.35%), Query Frame = 0

Query: 27  FFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEESEEIFCPSSEVTID---------- 86
           F CP+T +IM DPV + TG TYE+++I +WF+   ++    CP +   +D          
Sbjct: 294 FLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKT----CPKTRQELDHLSLAPNFAL 353

Query: 87  -----KWKERNEI--------------ARIKVARAALSLASSD-DMVLEAIKDLSSICKG 146
                +W E+N                 + +V+    +L+SS  +    ++K +  + + 
Sbjct: 354 KNLIMQWCEKNNFKIPEKEVSPDSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLARE 413

Query: 147 KQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMI 206
              N   I N   +PLLV+ L Y D  ++   +  L  ++ I+E NKK+I N+  +  +I
Sbjct: 414 NPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNLS-IDEVNKKLISNEGAIPNII 473

Query: 207 ILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFASERADET 266
            +L + +R  R  S   L  LS        IG ++ GI  L+ +          + A   
Sbjct: 474 EILENGNREARENSAAALFSLSMLDENKVTIG-LSNGIPPLVDL-LQHGTLRGKKDALTA 533

Query: 267 LRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERAS 326
           L NL  +  N     + G+++PLL +L +      I+ A  +  ++  H        + S
Sbjct: 534 LFNLSLNSANKGRAIDAGIVQPLL-NLLKDKNLGMIDEALSILLLLASHPEGRQAIGQLS 593

Query: 327 --PALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELN 371
               LV+ + +G    ++ A   LL++ S+  +  ILA    +Q  V E          N
Sbjct: 594 FIETLVEFIRQGTPKNKECATSVLLELGSNN-SSFILA---ALQFGVYEYLVEITTSGTN 653

BLAST of Sgr028994 vs. ExPASy TrEMBL
Match: A0A6J1D4U3 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111017601 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.4e-202
Identity = 371/448 (82.81%), Postives = 406/448 (90.62%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           MKYRNV   LTKLPS+AHYIEPLFETFFCPLTKDIMDDPVSLETG TYERQAIV+WFEE+
Sbjct: 205 MKYRNVTESLTKLPSMAHYIEPLFETFFCPLTKDIMDDPVSLETGATYERQAIVDWFEEY 264

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEEIFCP               + + TIDKWKERNEIARIKVARAALSLASSD+MVLE
Sbjct: 265 EESEEIFCPVTGQKLVSKAFNSNRALKSTIDKWKERNEIARIKVARAALSLASSDEMVLE 324

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AI+DL SICKGKQCN+ERIFNFDMLPLLVKFL+Y+D+DVRY VLELLQ+MAEI+ED KKM
Sbjct: 325 AIEDLLSICKGKQCNVERIFNFDMLPLLVKFLEYKDRDVRYGVLELLQQMAEIDEDKKKM 384

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           I NQLD+ RMI LLSSSH+ IRNTSLLL+LELSRSQSLSDQIGSVTG I MLITMKY+RS
Sbjct: 385 IYNQLDMLRMIKLLSSSHQFIRNTSLLLVLELSRSQSLSDQIGSVTGAIPMLITMKYNRS 444

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFAS +ADETLRNLERSPTNIKLMAEHGL++PLLR LTEGSEW RIEMASYLGEMVIEH
Sbjct: 445 DEFASRKADETLRNLERSPTNIKLMAEHGLLEPLLRYLTEGSEWMRIEMASYLGEMVIEH 504

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           +CM HVAE ASPALV+MVHEGD  +RKAAFKALLQISSHKPNGKILA AGTVQVMVEEMF
Sbjct: 505 NCMAHVAETASPALVKMVHEGDAFIRKAAFKALLQISSHKPNGKILAKAGTVQVMVEEMF 564

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK+EAARILANICES L+ E ++VN+HGSTMSS+YVV+NIID+L+NSTPD
Sbjct: 565 TRTICDELNDPKKEAARILANICESNLNPEILQVNSHGSTMSSEYVVYNIIDVLENSTPD 624

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           EFS+SL+RILLCLTKSPKP+ TIVSGVK
Sbjct: 625 EFSLSLVRILLCLTKSPKPIDTIVSGVK 652

BLAST of Sgr028994 vs. ExPASy TrEMBL
Match: A0A6J1HVV2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467344 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.8e-192
Identity = 361/449 (80.40%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV A  TK PS A+YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIV+WFEEF
Sbjct: 208 VKYRNVTAVSTKRPSTANYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVQWFEEF 267

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD M+LE
Sbjct: 268 EESEEVFCPVTGQKLVSKAFNSNRALKSTIDKWKERNEIATIKVARAALSLASSDKMMLE 327

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AIKDLSSI KGKQ NIERIF+FDMLPLLV+ L+YR+KDVRY VLELL +MAEINEDNKK 
Sbjct: 328 AIKDLSSISKGKQFNIERIFDFDMLPLLVECLEYRNKDVRYAVLELLHQMAEINEDNKKT 387

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICNQLD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 388 ICNQLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKNDRS 447

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGEMVI  
Sbjct: 448 DEFASEKADETLRNLEISPTNIKLMAEYGLMEPLVRHLTEGNEWMRIEMASYLGEMVIRQ 507

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 508 DCMAYVAEKASPALVGMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 567

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK EAA ILANIC+S LDLET++VN HGSTMSSDY+V NIIDLL  STPD
Sbjct: 568 TRTICDELNDPKTEAAEILANICDSSLDLETLQVNAHGSTMSSDYIVCNIIDLLDISTPD 627

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLCLTKSPK M TIVSGVK+
Sbjct: 628 EFSKSLIRILLCLTKSPKTMDTIVSGVKS 656

BLAST of Sgr028994 vs. ExPASy TrEMBL
Match: A0A6J1FZG7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449298 PE=4 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.5e-191
Identity = 359/449 (79.96%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 1   MKYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEF 60
           +KYRNV    TK PS A+YIEPLFETF CPLTKDIMDDPVSLETGVTYER+AIVEWFEEF
Sbjct: 208 VKYRNVTEVSTKRPSTANYIEPLFETFICPLTKDIMDDPVSLETGVTYERKAIVEWFEEF 267

Query: 61  EESEEIFCP---------------SSEVTIDKWKERNEIARIKVARAALSLASSDDMVLE 120
           EESEE+FCP               + + TIDKWKERNEIA IKVARAALSLASSD MVLE
Sbjct: 268 EESEEVFCPVTGQKLVSKAVNSNRALKSTIDKWKERNEIATIKVARAALSLASSDAMVLE 327

Query: 121 AIKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKM 180
           AI+DLSSI KGKQ NIERIF+F+MLPLLV+ L+YR+KDVRY VLELL +MAEINEDNKK+
Sbjct: 328 AIEDLSSISKGKQFNIERIFDFEMLPLLVECLEYRNKDVRYAVLELLHQMAEINEDNKKI 387

Query: 181 ICNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS 240
           ICNQLD+SRM+ LLSS HRSIRNTSLLLL ELSR+QSL+DQIGSVTGGISMLI MK DRS
Sbjct: 388 ICNQLDMSRMVNLLSSGHRSIRNTSLLLLHELSRTQSLTDQIGSVTGGISMLIVMKDDRS 447

Query: 241 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 300
           DEFASE+ADETLRNLE SPTNIKLMAE+GLM+PL+R LTEG+EW RIEMASYLGE+VI  
Sbjct: 448 DEFASEKADETLRNLETSPTNIKLMAEYGLMEPLVRYLTEGNEWMRIEMASYLGEIVIRQ 507

Query: 301 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 360
           DCM +VAE+ASPALV MVHEGDTLVR AAFKALLQISSH+PNG+ILA AGTVQVM EE+F
Sbjct: 508 DCMAYVAEKASPALVDMVHEGDTLVRNAAFKALLQISSHRPNGEILAKAGTVQVMAEEIF 567

Query: 361 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 420
           TRTICDELNDPK EAA ILANICES LDLET++VN HGSTMSSDY+V+NIIDLL  STPD
Sbjct: 568 TRTICDELNDPKTEAAEILANICESSLDLETLQVNAHGSTMSSDYIVYNIIDLLDISTPD 627

Query: 421 EFSMSLIRILLCLTKSPKPMATIVSGVKT 435
           EFS SLIRILLCLTKSPK M TIV GVK+
Sbjct: 628 EFSKSLIRILLCLTKSPKTMDTIVLGVKS 656

BLAST of Sgr028994 vs. ExPASy TrEMBL
Match: A0A6J1B3J7 (RING-type E3 ubiquitin transferase OS=Herrania umbratica OX=108875 GN=LOC110423335 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 4.9e-139
Identity = 264/447 (59.06%), Postives = 345/447 (77.18%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           K+ N+   LT LP VAHYIEPL++TFFCPLTK IMDDPV++E+GVTYER+AI++WFE F+
Sbjct: 220 KHENINKSLTTLPQVAHYIEPLYDTFFCPLTKQIMDDPVTIESGVTYEREAIMDWFEMFD 279

Query: 62  ESEEIFCPSS---------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEA 121
             E+  CP++               + TI++WK+RNE ARIKVARAAL+LASSD M+LEA
Sbjct: 280 HLEDKVCPTTGKKLTSRVLSTNVALKTTIEEWKDRNEAARIKVARAALTLASSDSMILEA 339

Query: 122 IKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMI 181
           I+DL  IC+ KQ N   + +  +LPLL+KFL Y+D D+R   LELL+++ E +++ K+MI
Sbjct: 340 IRDLQHICQRKQYNKVHVLSVGILPLLIKFLGYKDGDIRCAALELLRQLVEEDDEGKEMI 399

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              +D+S +I LLSSSH+ IR+ SLL LLELSRSQ+L ++IGS TG I MLI +KY+R+ 
Sbjct: 400 AKTMDISTIIELLSSSHQQIRHASLLFLLELSRSQALGEKIGSATGAILMLIRIKYNRNV 459

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASERADE L+NLE  P NIK MAE+G ++PLL  LTEGSE  ++EMA+YLGE+++ H
Sbjct: 460 DSFASERADEILKNLEGYPDNIKQMAEYGFLEPLLNHLTEGSEEVQMEMANYLGEIILGH 519

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           D  T+VAERASP+L++MV  G++++RKAAFKAL QISS+ PNG IL  AG V +M EE+F
Sbjct: 520 DSETYVAERASPSLMKMVQSGNSIIRKAAFKALAQISSYHPNGGILVEAGIVNIMAEELF 579

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
            R I DE  + K+EAA ILANI ES ++ + ++VNTHG  +SSDYVV+NII +LKNSTPD
Sbjct: 580 IRRIYDEPMNSKKEAAAILANILESGVEHDNIQVNTHGLRISSDYVVYNIIYMLKNSTPD 639

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGV 433
           E +++LIRILLCLTKSPKPMATI+S V
Sbjct: 640 ELNINLIRILLCLTKSPKPMATIISVV 666

BLAST of Sgr028994 vs. ExPASy TrEMBL
Match: A0A6J1B222 (RING-type E3 ubiquitin transferase OS=Herrania umbratica OX=108875 GN=LOC110423335 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 4.9e-139
Identity = 264/447 (59.06%), Postives = 345/447 (77.18%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           K+ N+   LT LP VAHYIEPL++TFFCPLTK IMDDPV++E+GVTYER+AI++WFE F+
Sbjct: 215 KHENINKSLTTLPQVAHYIEPLYDTFFCPLTKQIMDDPVTIESGVTYEREAIMDWFEMFD 274

Query: 62  ESEEIFCPSS---------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEA 121
             E+  CP++               + TI++WK+RNE ARIKVARAAL+LASSD M+LEA
Sbjct: 275 HLEDKVCPTTGKKLTSRVLSTNVALKTTIEEWKDRNEAARIKVARAALTLASSDSMILEA 334

Query: 122 IKDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMI 181
           I+DL  IC+ KQ N   + +  +LPLL+KFL Y+D D+R   LELL+++ E +++ K+MI
Sbjct: 335 IRDLQHICQRKQYNKVHVLSVGILPLLIKFLGYKDGDIRCAALELLRQLVEEDDEGKEMI 394

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              +D+S +I LLSSSH+ IR+ SLL LLELSRSQ+L ++IGS TG I MLI +KY+R+ 
Sbjct: 395 AKTMDISTIIELLSSSHQQIRHASLLFLLELSRSQALGEKIGSATGAILMLIRIKYNRNV 454

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASERADE L+NLE  P NIK MAE+G ++PLL  LTEGSE  ++EMA+YLGE+++ H
Sbjct: 455 DSFASERADEILKNLEGYPDNIKQMAEYGFLEPLLNHLTEGSEEVQMEMANYLGEIILGH 514

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           D  T+VAERASP+L++MV  G++++RKAAFKAL QISS+ PNG IL  AG V +M EE+F
Sbjct: 515 DSETYVAERASPSLMKMVQSGNSIIRKAAFKALAQISSYHPNGGILVEAGIVNIMAEELF 574

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
            R I DE  + K+EAA ILANI ES ++ + ++VNTHG  +SSDYVV+NII +LKNSTPD
Sbjct: 575 IRRIYDEPMNSKKEAAAILANILESGVEHDNIQVNTHGLRISSDYVVYNIIYMLKNSTPD 634

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGV 433
           E +++LIRILLCLTKSPKPMATI+S V
Sbjct: 635 ELNINLIRILLCLTKSPKPMATIISVV 661

BLAST of Sgr028994 vs. TAIR 10
Match: AT1G68940.1 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.4e-116
Identity = 226/448 (50.45%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           KY N++  L+ LP V  ++EP ++ F CPLTK+IM+DPV+ ETGVT ERQA++EWF+ F 
Sbjct: 225 KYGNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFG 284

Query: 62  ESEEIFCPSS--------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEAI 121
            S+EI CP +              +  I +WK RNE ARIKVA AALSL  S+ MV++A+
Sbjct: 285 NSDEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 344

Query: 122 KDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMA-EINEDNKKMI 181
           +DL   C+GK+ N  ++    ++ LL ++L YR KDVR+E+L+ L+ +A E  +D K+MI
Sbjct: 345 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 404

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              + +S +I LL SSH+ +R+ +  LLLELS+SQ   ++IG+  G I ML+T KY+R  
Sbjct: 405 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 464

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASE +D+ LRNLE+ P NIK MAE GL++PLL  L EGSE T++ MA+YL E+ I H
Sbjct: 465 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGH 524

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           +  T+VAE+A PAL+ +V   +   R+AAFKAL  IS + PN KIL   G +++MVEEMF
Sbjct: 525 EKKTYVAEKACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 584

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
           T+ +  +L + + EAA ILANI ES L+ ET  VNTHG T+ SDY V+NII +LKNS+PD
Sbjct: 585 TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 644

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           + ++ LIRILL L+KSP+ MATIVS +K
Sbjct: 645 DLNIDLIRILLSLSKSPRAMATIVSVIK 672

BLAST of Sgr028994 vs. TAIR 10
Match: AT1G68940.2 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.4e-116
Identity = 226/448 (50.45%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           KY N++  L+ LP V  ++EP ++ F CPLTK+IM+DPV+ ETGVT ERQA++EWF+ F 
Sbjct: 225 KYGNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFG 284

Query: 62  ESEEIFCPSS--------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEAI 121
            S+EI CP +              +  I +WK RNE ARIKVA AALSL  S+ MV++A+
Sbjct: 285 NSDEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 344

Query: 122 KDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMA-EINEDNKKMI 181
           +DL   C+GK+ N  ++    ++ LL ++L YR KDVR+E+L+ L+ +A E  +D K+MI
Sbjct: 345 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 404

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              + +S +I LL SSH+ +R+ +  LLLELS+SQ   ++IG+  G I ML+T KY+R  
Sbjct: 405 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 464

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASE +D+ LRNLE+ P NIK MAE GL++PLL  L EGSE T++ MA+YL E+ I H
Sbjct: 465 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGH 524

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           +  T+VAE+A PAL+ +V   +   R+AAFKAL  IS + PN KIL   G +++MVEEMF
Sbjct: 525 EKKTYVAEKACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 584

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
           T+ +  +L + + EAA ILANI ES L+ ET  VNTHG T+ SDY V+NII +LKNS+PD
Sbjct: 585 TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 644

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           + ++ LIRILL L+KSP+ MATIVS +K
Sbjct: 645 DLNIDLIRILLSLSKSPRAMATIVSVIK 672

BLAST of Sgr028994 vs. TAIR 10
Match: AT1G68940.3 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.4e-116
Identity = 226/448 (50.45%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 2   KYRNVAAPLTKLPSVAHYIEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFE 61
           KY N++  L+ LP V  ++EP ++ F CPLTK+IM+DPV+ ETGVT ERQA++EWF+ F 
Sbjct: 225 KYGNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFG 284

Query: 62  ESEEIFCPSS--------------EVTIDKWKERNEIARIKVARAALSLASSDDMVLEAI 121
            S+EI CP +              +  I +WK RNE ARIKVA AALSL  S+ MV++A+
Sbjct: 285 NSDEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 344

Query: 122 KDLSSICKGKQCNIERIFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMA-EINEDNKKMI 181
           +DL   C+GK+ N  ++    ++ LL ++L YR KDVR+E+L+ L+ +A E  +D K+MI
Sbjct: 345 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 404

Query: 182 CNQLDLSRMIILLSSSHRSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRS- 241
              + +S +I LL SSH+ +R+ +  LLLELS+SQ   ++IG+  G I ML+T KY+R  
Sbjct: 405 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 464

Query: 242 DEFASERADETLRNLERSPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEH 301
           D FASE +D+ LRNLE+ P NIK MAE GL++PLL  L EGSE T++ MA+YL E+ I H
Sbjct: 465 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGH 524

Query: 302 DCMTHVAERASPALVQMVHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMF 361
           +  T+VAE+A PAL+ +V   +   R+AAFKAL  IS + PN KIL   G +++MVEEMF
Sbjct: 525 EKKTYVAEKACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 584

Query: 362 TRTICDELNDPKREAARILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPD 421
           T+ +  +L + + EAA ILANI ES L+ ET  VNTHG T+ SDY V+NII +LKNS+PD
Sbjct: 585 TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 644

Query: 422 EFSMSLIRILLCLTKSPKPMATIVSGVK 434
           + ++ LIRILL L+KSP+ MATIVS +K
Sbjct: 645 DLNIDLIRILLSLSKSPRAMATIVSVIK 672

BLAST of Sgr028994 vs. TAIR 10
Match: AT1G76390.1 (ARM repeat superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-59
Identity = 148/468 (31.62%), Postives = 261/468 (55.77%), Query Frame = 0

Query: 20  IEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEES-EEIFCP--SSEV--- 79
           I+ ++E F CPLTK +M +PV+LE G T+ER+AI +WF+E  E+ + + CP  S E+   
Sbjct: 22  IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKWFQECRENGQPLSCPITSKELSIT 81

Query: 80  ----------TIDKWKERNEIARIKVARAALSLASSDDMVLEAIKDLSSICKGKQCNIER 139
                     TI++W+ RN+  ++ +AR +L L +++  +L A+K++  IC+  +   +R
Sbjct: 82  DLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVREICRNIRKIRQR 141

Query: 140 IFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMIILLSSSH 199
           + N  ++ L+   L     +VR + L+ LQ + E +E++K ++     +  ++  LS   
Sbjct: 142 VCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDTVRTIVKFLSQEP 201

Query: 200 RSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFAS-ERADETLRNLER 259
              R  ++ +L ELS+S++L ++IGS+ G I +L+ +   +S+  ++ E+AD+TL NLER
Sbjct: 202 SKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTVEKADKTLTNLER 261

Query: 260 SPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERASPALVQM 319
           S  N++ MA +G ++PLL  L EGS  T++ MA YLG + + +D    VA+    +L+ +
Sbjct: 262 SEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVIVAQTVGSSLIDL 321

Query: 320 VHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELNDPKREAAR 379
           +   D   R+AA  AL  ISS + + K+L N G +  +++++F           K  +A 
Sbjct: 322 MRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGPNQLPIRLKEVSAT 381

Query: 380 ILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPDEFSMSLIRILLCLTKSP 439
           ILANI     D + V V  H  T+ S+ +V N++ L  N+ P E    L+ +L+ LT  P
Sbjct: 382 ILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGP-EIQGKLLAVLVGLTSCP 441

Query: 440 KPMATIVSGVKTLKQVIPSLSSSIVQMRNLECSNKTAYITVPLHGLHN 471
             +  +VS ++    +I     S+VQ   +  ++     ++ L  LHN
Sbjct: 442 NSVINVVSAIRNSAAII-----SLVQFVEIHENDDLRLASIKL--LHN 481

BLAST of Sgr028994 vs. TAIR 10
Match: AT1G76390.2 (ARM repeat superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-59
Identity = 148/468 (31.62%), Postives = 261/468 (55.77%), Query Frame = 0

Query: 20  IEPLFETFFCPLTKDIMDDPVSLETGVTYERQAIVEWFEEFEES-EEIFCP--SSEV--- 79
           I+ ++E F CPLTK +M +PV+LE G T+ER+AI +WF+E  E+ + + CP  S E+   
Sbjct: 22  IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKWFQECRENGQPLSCPITSKELSIT 81

Query: 80  ----------TIDKWKERNEIARIKVARAALSLASSDDMVLEAIKDLSSICKGKQCNIER 139
                     TI++W+ RN+  ++ +AR +L L +++  +L A+K++  IC+  +   +R
Sbjct: 82  DLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVREICRNIRKIRQR 141

Query: 140 IFNFDMLPLLVKFLDYRDKDVRYEVLELLQEMAEINEDNKKMICNQLDLSRMIILLSSSH 199
           + N  ++ L+   L     +VR + L+ LQ + E +E++K ++     +  ++  LS   
Sbjct: 142 VCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDTVRTIVKFLSQEP 201

Query: 200 RSIRNTSLLLLLELSRSQSLSDQIGSVTGGISMLITMKYDRSDEFAS-ERADETLRNLER 259
              R  ++ +L ELS+S++L ++IGS+ G I +L+ +   +S+  ++ E+AD+TL NLER
Sbjct: 202 SKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTVEKADKTLTNLER 261

Query: 260 SPTNIKLMAEHGLMKPLLRDLTEGSEWTRIEMASYLGEMVIEHDCMTHVAERASPALVQM 319
           S  N++ MA +G ++PLL  L EGS  T++ MA YLG + + +D    VA+    +L+ +
Sbjct: 262 SEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVIVAQTVGSSLIDL 321

Query: 320 VHEGDTLVRKAAFKALLQISSHKPNGKILANAGTVQVMVEEMFTRTICDELNDPKREAAR 379
           +   D   R+AA  AL  ISS + + K+L N G +  +++++F           K  +A 
Sbjct: 322 MRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGPNQLPIRLKEVSAT 381

Query: 380 ILANICESCLDLETVRVNTHGSTMSSDYVVHNIIDLLKNSTPDEFSMSLIRILLCLTKSP 439
           ILANI     D + V V  H  T+ S+ +V N++ L  N+ P E    L+ +L+ LT  P
Sbjct: 382 ILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGP-EIQGKLLAVLVGLTSCP 441

Query: 440 KPMATIVSGVKTLKQVIPSLSSSIVQMRNLECSNKTAYITVPLHGLHN 471
             +  +VS ++    +I     S+VQ   +  ++     ++ L  LHN
Sbjct: 442 NSVINVVSAIRNSAAII-----SLVQFVEIHENDDLRLASIKL--LHN 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149110.14.9e-20282.81putative U-box domain-containing protein 42 [Momordica charantia][more]
XP_023542844.15.5e-19380.62putative U-box domain-containing protein 42 [Cucurbita pepo subsp. pepo][more]
XP_022967965.17.9e-19280.40putative U-box domain-containing protein 42 [Cucurbita maxima] >XP_022967966.1 p... [more]
XP_022944907.15.1e-19179.96putative U-box domain-containing protein 42 [Cucurbita moschata][more]
KAG6574024.14.3e-19079.51putative U-box domain-containing protein 42, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
Q9CAA74.7e-11550.45Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9SFX21.8e-5831.62U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2... [more]
Q9LM762.9e-5631.20U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1... [more]
Q10FT05.5e-4732.08U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q681N22.0e-1224.20U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1D4U32.4e-20282.81RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101760... [more]
A0A6J1HVV23.8e-19280.40RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467344 P... [more]
A0A6J1FZG72.5e-19179.96RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449298... [more]
A0A6J1B3J74.9e-13959.06RING-type E3 ubiquitin transferase OS=Herrania umbratica OX=108875 GN=LOC1104233... [more]
A0A6J1B2224.9e-13959.06RING-type E3 ubiquitin transferase OS=Herrania umbratica OX=108875 GN=LOC1104233... [more]
Match NameE-valueIdentityDescription
AT1G68940.13.4e-11650.45Armadillo/beta-catenin-like repeat family protein [more]
AT1G68940.23.4e-11650.45Armadillo/beta-catenin-like repeat family protein [more]
AT1G68940.33.4e-11650.45Armadillo/beta-catenin-like repeat family protein [more]
AT1G76390.11.3e-5931.62ARM repeat superfamily protein [more]
AT1G76390.21.3e-5931.62ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 26..92
e-value: 3.6E-8
score: 43.1
IPR003613U box domainPFAMPF04564U-boxcoord: 25..59
e-value: 1.1E-6
score: 28.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 22..99
score: 11.067736
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 82..287
e-value: 4.5E-22
score: 80.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 288..452
e-value: 7.2E-11
score: 43.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 21..81
e-value: 2.3E-13
score: 51.3
NoneNo IPR availablePANTHERPTHR45958RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 3..434
NoneNo IPR availablePANTHERPTHR45958:SF4U-BOX DOMAIN-CONTAINING PROTEIN 42-RELATEDcoord: 3..434
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 14..88
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 93..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr028994.1Sgr028994.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity