Sgr028958 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028958 vs. NCBI nr
Match: XP_023541647.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 1.8e-191
Identity = 366/444 (82.43%), Postives = 375/444 (84.46%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRPAV NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPAVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKLKAIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLKAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSL  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. NCBI nr
Match: XP_038892144.1 (mediator of RNA polymerase II transcription subunit 25 isoform X2 [Benincasa hispida])

HSP 1 Score: 679.1 bits (1751), Expect = 2.3e-191
Identity = 363/439 (82.69%), Postives = 374/439 (85.19%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQT+VSDYIEKIVR  CGNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTIVSDYIEKIVRSFCGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD F QWLSAIPFSGGGFSD AIAEGLAEALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDIFTQWLSAIPFSGGGFSDGAIAEGLAEALMPNGGQNQQTMDM 120

Query: 121 -KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCFISLSV 180
            KHCILVAASNPYPLPTPVYRPAVQNLEQHDN EP  G+SQSYAETVAKSF QCFISLSV
Sbjct: 121 QKHCILVAASNPYPLPTPVYRPAVQNLEQHDNGEP--GSSQSYAETVAKSFSQCFISLSV 180

Query: 181 ICPKQLPKLKAIYNA-----------------------------VTRAALSRPGITSLPT 240
           ICPKQLPKL+AIY A                               RAALSRPGITSLP 
Sbjct: 181 ICPKQLPKLRAIYTAGKRNPRAADPPIDNVKTPSYLVLISENFVEARAALSRPGITSLPA 240

Query: 241 NQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTSMANGS 300
           NQSPVKMDI+PV PVTGPP TT  SVNGP+INRQPVSVANVPPATVKVEPNTVTSM NGS
Sbjct: 241 NQSPVKMDISPVVPVTGPPTTTTPSVNGPIINRQPVSVANVPPATVKVEPNTVTSMTNGS 300

Query: 301 AYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPGGPANV 360
            YPPHMP+VVRAASQGVPS+QTSSPLSSQEMITNNENTQDLKPLVNGVPQP+RP GPANV
Sbjct: 301 GYPPHMPSVVRAASQGVPSMQTSSPLSSQEMITNNENTQDLKPLVNGVPQPVRPVGPANV 360

Query: 361 SILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNVFSSGQ 398
           SILNNISQARVM+ AAL+GGTSIGL SMGQTPMAMHVSNMISSGMGSSVPAAQNVFSSGQ
Sbjct: 361 SILNNISQARVMSTAALNGGTSIGLPSMGQTPMAMHVSNMISSGMGSSVPAAQNVFSSGQ 420

BLAST of Sgr028958 vs. NCBI nr
Match: XP_038892143.1 (mediator of RNA polymerase II transcription subunit 25 isoform X1 [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 8.8e-191
Identity = 363/444 (81.76%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQT+VSDYIEKIVR  CGNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTIVSDYIEKIVRSFCGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD F QWLSAIPFSGGGFSD AIAEGLAEALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDIFTQWLSAIPFSGGGFSDGAIAEGLAEALMMFSTQPNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRPAVQNLEQHDN EP  G+SQSYAETVAKSF QCF
Sbjct: 121 QTMDMQKHCILVAASNPYPLPTPVYRPAVQNLEQHDNGEP--GSSQSYAETVAKSFSQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIY A                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNVKTPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSLP NQSPVKMDI+PV PVTGPP TT  SVNGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLPANQSPVKMDISPVVPVTGPPTTTTPSVNGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGS YPPHMP+VVRAASQGVPS+QTSSPLSSQEMITNNENTQDLKPLVNGVPQP+RP 
Sbjct: 301 MTNGSGYPPHMPSVVRAASQGVPSMQTSSPLSSQEMITNNENTQDLKPLVNGVPQPVRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARVM+ AAL+GGTSIGL SMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARVMSTAALNGGTSIGLPSMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. NCBI nr
Match: XP_022945397.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 364/444 (81.98%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRP V NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSL  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. NCBI nr
Match: XP_022945395.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 364/444 (81.98%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRP V NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSL  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. ExPASy Swiss-Prot
Match: Q7XYY2 (Mediator of RNA polymerase II transcription subunit 25 OS=Arabidopsis thaliana OX=3702 GN=MED25 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.1e-114
Identity = 234/439 (53.30%), Postives = 301/439 (68.56%), Query Frame = 0

Query: 4   KQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTHGSY 63
           KQLIVV EGTAA+GP+WQT+VSDY+EKI+R  CG+EL G++ P S VE SLV F++HGSY
Sbjct: 6   KQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSY 65

Query: 64  CGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM--------------- 123
           C CLVQR+GWTRDVD F+ WLS+I F GGGF++ A AEGLAEALM               
Sbjct: 66  CACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDL 125

Query: 124 -KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCFISLSV 183
            +HCIL+ ASNP+ LPTPVYRP +QN+E+++N + Q+ +  S AETVA  F +C +SLSV
Sbjct: 126 KRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSV 185

Query: 184 ICPKQLPKLKAIYNA----------------------------VTRAALSRPGITSLPTN 243
           +CPKQLP ++A+YNA                            V   A      T+LP  
Sbjct: 186 VCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQT 245

Query: 244 QSPVKMD---ITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTSMAN 303
           QSPVK+D   + P  PVTG PP  +SS NGP+ NRQPVSV  VP ATVKVEP+TVTSMA 
Sbjct: 246 QSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAP 305

Query: 304 GSAYPPHMPTVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKP-LVNGVPQPLRPGG 363
             ++ PH+P V R A+Q +PS+QTSS    SQ+M++N EN  D+KP +V+G+  PLR G 
Sbjct: 306 VPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGP 365

Query: 364 P--ANVSILNNISQAR-VMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQ 391
           P  ANV++LNN+SQ R VM+ AAL+G  S    S+GQ+ +AMH+SNMIS+GM +S+P +Q
Sbjct: 366 PGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVAMHMSNMISTGMATSLPPSQ 425

BLAST of Sgr028958 vs. ExPASy Swiss-Prot
Match: A0A3Q7JC00 (Mediator of RNA polymerase II transcription subunit 25 OS=Solanum lycopersicum OX=4081 GN=MED25 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.4e-113
Identity = 235/442 (53.17%), Postives = 303/442 (68.55%), Query Frame = 0

Query: 5   QLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTHGSYC 64
           +LIV VEGTA +GP+W+ +VSDY++KI+RC  G + T QK   ++VE S+V F+THG Y 
Sbjct: 4   KLIVAVEGTAVLGPYWKIIVSDYLDKIIRCFFGVDSTSQKSSAADVEVSMVMFNTHGPYS 63

Query: 65  GCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM---------------- 124
            CLVQR+GWT+D+D+F+QWLSAIPFSGGGF+DAA+AEGLAEAL+                
Sbjct: 64  ACLVQRSGWTKDMDTFLQWLSAIPFSGGGFNDAAVAEGLAEALVMFSVPNGNQTQQKMEG 123

Query: 125 -KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCFISLSV 184
            KHCIL++ SNPYPLPTPVYRP +Q LEQ++N+E Q+ +  + AETVAK+F QC ISLSV
Sbjct: 124 KKHCILISGSNPYPLPTPVYRPQMQKLEQNENIEAQTDSRLADAETVAKTFPQCSISLSV 183

Query: 185 ICPKQLPKLKAIYNA-----------------------------VTRAALSRPGITSLPT 244
           ICPK+LPKL+AIY+A                               RAA SR G+T+L +
Sbjct: 184 ICPKKLPKLRAIYDAGKHNPRAADPPIDTAKNPNFLVLISENFIEARAAFSRSGLTNLAS 243

Query: 245 NQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTSMANGS 304
           N SPVKMD++ V PV+G    + S+ N  +I+R P+S  N+PPATVK+EPNTVT M  G 
Sbjct: 244 NHSPVKMDVSSVLPVSGTQSISNSAANVSVISRPPISAGNIPPATVKIEPNTVTPM-TGP 303

Query: 305 AYPPHMPTVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKPLVNGVPQPLRP--GGP 364
            +  H+P+ VR A Q VPSLQ SSPLS SQEM+++ EN Q++KP+V+G+ Q LRP     
Sbjct: 304 GF-SHIPS-VRPALQPVPSLQASSPLSVSQEMVSHTENVQEMKPIVSGMTQSLRPVAAAA 363

Query: 365 ANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNVFS 398
           ANV ILN ++QA       L GGTSIGL SMG TPM   +S+MISSGM SSVPA+Q V S
Sbjct: 364 ANVKILNGVAQAH----QVLGGGTSIGLQSMGGTPM---LSSMISSGMASSVPASQAVLS 423

BLAST of Sgr028958 vs. ExPASy TrEMBL
Match: A0A6J1G0T3 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita moschata OX=3662 GN=LOC111449642 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.6e-191
Identity = 364/444 (81.98%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRP V NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSL  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. ExPASy TrEMBL
Match: A0A6J1G0U0 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita moschata OX=3662 GN=LOC111449642 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.6e-191
Identity = 364/444 (81.98%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRP V NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSL  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. ExPASy TrEMBL
Match: A0A6J1L0S1 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita maxima OX=3661 GN=LOC111497789 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 9.5e-191
Identity = 364/444 (81.98%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRC  GNE TGQKPPTSNVEFSLV FHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCFSGNETTGQKPPTSNVEFSLVMFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLAEALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLAEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEP  G+SQSYAETVAKSF QCF
Sbjct: 121 QTMDMQKHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEP--GSSQSYAETVAKSFPQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIY A                               RAAL+RPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYTAGKRNLRSADPPIDNVKTPSYLVLISENFVEARAALNRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSLP NQSPVKMDI+PV PVTGPPPTT  SVNGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSVNGPVINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNE+TQDLKPLVNGVPQ LRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNESTQDLKPLVNGVPQTLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARVM+ A L+GG SIGL SMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARVMSSATLNGGNSIGLTSMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. ExPASy TrEMBL
Match: A0A5D3CFF1 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00760 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.6e-190
Identity = 361/444 (81.31%), Postives = 375/444 (84.46%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MA+KQLIVVVEGTAAMGPFWQTVVSDYIEKIVR  CGNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MADKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRSFCGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRDVD F+QWLSAIPFSGGGFSDAAIAEGLAEALM            
Sbjct: 61  GSYCGCLVQRTGWTRDVDIFMQWLSAIPFSGGGFSDAAIAEGLAEALMMFPTQPNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCIL+AASNPYPLPTPVYRPAVQNLEQHDNVEP  G+SQSYAETVAKSF QCF
Sbjct: 121 QTMDMQKHCILIAASNPYPLPTPVYRPAVQNLEQHDNVEP--GSSQSYAETVAKSFSQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIY A                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNVKTPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           TSLP NQSPVKMDI+PV PVTGPPPTT  SVNGP+INRQPVSV N PPATVKVEPNTVTS
Sbjct: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSVNGPIINRQPVSVTNGPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGS YPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQP+R  
Sbjct: 301 MTNGSGYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPVRSL 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARVM+ AAL+GGTSIGL SMGQTP+AMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARVMSTAALNGGTSIGLPSMGQTPIAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. ExPASy TrEMBL
Match: A0A6J1HWR7 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita maxima OX=3661 GN=LOC111467555 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.6e-190
Identity = 362/444 (81.53%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVV+DYIEKIVRC  GNELTGQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM------------ 120
           GSYCGCLVQRTGWTRD D+FIQWLSAIPFSGGGFSDAAIAEGL EALM            
Sbjct: 61  GSYCGCLVQRTGWTRDADTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 ------KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCF 180
                 KHCILVAASNPYPLPTPVYRP V NLEQ DNVEPQSG+SQSYAETVAKSF QCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLKAIYNA-----------------------------VTRAALSRPGI 240
           ISLSVICPKQLPKL+AIYNA                               RAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPTNQSPVKMDITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTS 300
           T+L  NQSPVKMDI+PV PVTGPPPTT  S NGP+INRQPVSVANVPPATVKVEPNTVTS
Sbjct: 241 TTLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MANGSAYPPHMPTVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVPQPLRPG 360
           M NGSAYPPHMP+VVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGV QPLRP 
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 398
           GPANVSILNNISQARV   A L+GGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

BLAST of Sgr028958 vs. TAIR 10
Match: AT1G25540.1 (phytochrome and flowering time regulatory protein (PFT1) )

HSP 1 Score: 413.3 bits (1061), Expect = 2.2e-115
Identity = 234/439 (53.30%), Postives = 301/439 (68.56%), Query Frame = 0

Query: 4   KQLIVVVEGTAAMGPFWQTVVSDYIEKIVRCLCGNELTGQKPPTSNVEFSLVTFHTHGSY 63
           KQLIVV EGTAA+GP+WQT+VSDY+EKI+R  CG+EL G++ P S VE SLV F++HGSY
Sbjct: 6   KQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSY 65

Query: 64  CGCLVQRTGWTRDVDSFIQWLSAIPFSGGGFSDAAIAEGLAEALM--------------- 123
           C CLVQR+GWTRDVD F+ WLS+I F GGGF++ A AEGLAEALM               
Sbjct: 66  CACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDL 125

Query: 124 -KHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCFISLSV 183
            +HCIL+ ASNP+ LPTPVYRP +QN+E+++N + Q+ +  S AETVA  F +C +SLSV
Sbjct: 126 KRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSV 185

Query: 184 ICPKQLPKLKAIYNA----------------------------VTRAALSRPGITSLPTN 243
           +CPKQLP ++A+YNA                            V   A      T+LP  
Sbjct: 186 VCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQT 245

Query: 244 QSPVKMD---ITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTSMAN 303
           QSPVK+D   + P  PVTG PP  +SS NGP+ NRQPVSV  VP ATVKVEP+TVTSMA 
Sbjct: 246 QSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAP 305

Query: 304 GSAYPPHMPTVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKP-LVNGVPQPLRPGG 363
             ++ PH+P V R A+Q +PS+QTSS    SQ+M++N EN  D+KP +V+G+  PLR G 
Sbjct: 306 VPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGP 365

Query: 364 P--ANVSILNNISQAR-VMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQ 391
           P  ANV++LNN+SQ R VM+ AAL+G  S    S+GQ+ +AMH+SNMIS+GM +S+P +Q
Sbjct: 366 PGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVAMHMSNMISTGMATSLPPSQ 425

BLAST of Sgr028958 vs. TAIR 10
Match: AT1G25540.2 (phytochrome and flowering time regulatory protein (PFT1) )

HSP 1 Score: 260.0 bits (663), Expect = 3.1e-69
Identity = 161/320 (50.31%), Postives = 213/320 (66.56%), Query Frame = 0

Query: 107 LMKHCILVAASNPYPLPTPVYRPAVQNLEQHDNVEPQSGNSQSYAETVAKSFVQCFISLS 166
           L +HCIL+ ASNP+ LPTPVYRP +QN+E+++N + Q+ +  S AETVA  F +C +SLS
Sbjct: 16  LKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLS 75

Query: 167 VICPKQLPKLKAIYNA----------------------------VTRAALSRPGITSLPT 226
           V+CPKQLP ++A+YNA                            V   A      T+LP 
Sbjct: 76  VVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQ 135

Query: 227 NQSPVKMD---ITPVAPVTGPPPTTISSVNGPMINRQPVSVANVPPATVKVEPNTVTSMA 286
            QSPVK+D   + P  PVTG PP  +SS NGP+ NRQPVSV  VP ATVKVEP+TVTSMA
Sbjct: 136 TQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMA 195

Query: 287 NGSAYPPHMPTVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKP-LVNGVPQPLRPG 346
              ++ PH+P V R A+Q +PS+QTSS    SQ+M++N EN  D+KP +V+G+  PLR G
Sbjct: 196 PVPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTG 255

Query: 347 GP--ANVSILNNISQAR-VMNPAALSGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAA 391
            P  ANV++LNN+SQ R VM+ AAL+G  S    S+GQ+ +AMH+SNMIS+GM +S+P +
Sbjct: 256 PPGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVAMHMSNMISTGMATSLPPS 315