Sgr028937 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr028937
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionadenylate isopentenyltransferase-like
Locationtig00153210: 1795606 .. 1796670 (-)
RNA-Seq ExpressionSgr028937
SyntenySgr028937
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGTTGATTCAACATTTTCCTTGAAACTTATGACACTCCCTTCTCCAATTGCTTCTTCTTCTTCCCATTTCTATTCTTCAACTAAAATCTCCCTCCGTTTTCCCTTCTCCCCCAGACCCGCATGGCCTCGCATCAACGCCACTGCCTCCGCCGCTCGCCTTCTTCCTTCCGACGACAAGCTCCTCGTCATTATGGGCGCCACCGGCTGCGGCAAATCCGGCCTCTCCGTCCAGCTCGCCTCTCGTTTTCCCTCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAACGGCCTTGATATTACTACCAACAAGATTCCCCTCCACGAACGAAACGGCGTCCCCCACCACCTTCTTGGCGACTACGATTCTCTCGCCGGCGAATTCACTCCGCTCGACTTCCGAATCCATGCCGGCAATGCCGTCTCCGATATAGCTTCCAGGATGAAACTGCCCATCCTTGTTGGTGGATCCAATTCTTTTATCCATGCAATGCTGGCGAACCATTTCGATCCAAACGACGACGTCTTCGACGGTTCCCGATTCATCTCCTCCGATTTACGGTACCGTTGTTGTTTCCTCTGGCTGGACGTTGCGTTCCCAGTTTTATCAGAATACTTATCGATCAGAGTCGACGACATGCTGGAGGTCGGGATGTTCGAGGAACTAGCTGAGTTTTACAACCCGGACGAGGCCGATACGGCGCCGTATAGCGGGATAAGAAAAGCCATTGGAGTGCCGGAGTTCGACAGGTACTTCAAAAAATACCCACCGGATAAAAGAAGAATCCCGCAGAAATATCCCCGGGAGATTCGCAAGCGTAGGGAAGCATTCGAAGAAGCGGTGAATGCAATCAAGATTAACACGCATCTGCTGGCGGAGAAACAGATTGGGAAGATCTTGCGACTGAAGCGGGCCGGATGGGACATAAACGTATTGAATGCGACGGAGGCGTTTAGAGCGGTGGTGAATCCGAAACCCGGGCGGAACCGGCCGGAGATATGGGAGAAGCAGGTGTTGGAGCCAAGTTTGAAAATCGTAAAACGCTTCTTGGAGGAGTAG

mRNA sequence

ATGTGTGTTGATTCAACATTTTCCTTGAAACTTATGACACTCCCTTCTCCAATTGCTTCTTCTTCTTCCCATTTCTATTCTTCAACTAAAATCTCCCTCCGTTTTCCCTTCTCCCCCAGACCCGCATGGCCTCGCATCAACGCCACTGCCTCCGCCGCTCGCCTTCTTCCTTCCGACGACAAGCTCCTCGTCATTATGGGCGCCACCGGCTGCGGCAAATCCGGCCTCTCCGTCCAGCTCGCCTCTCGTTTTCCCTCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAACGGCCTTGATATTACTACCAACAAGATTCCCCTCCACGAACGAAACGGCGTCCCCCACCACCTTCTTGGCGACTACGATTCTCTCGCCGGCGAATTCACTCCGCTCGACTTCCGAATCCATGCCGGCAATGCCGTCTCCGATATAGCTTCCAGGATGAAACTGCCCATCCTTGTTGGTGGATCCAATTCTTTTATCCATGCAATGCTGGCGAACCATTTCGATCCAAACGACGACGTCTTCGACGGTTCCCGATTCATCTCCTCCGATTTACGGTACCGTTGTTGTTTCCTCTGGCTGGACGTTGCGTTCCCAGTTTTATCAGAATACTTATCGATCAGAGTCGACGACATGCTGGAGGTCGGGATGTTCGAGGAACTAGCTGAGTTTTACAACCCGGACGAGGCCGATACGGCGCCGTATAGCGGGATAAGAAAAGCCATTGGAGTGCCGGAGTTCGACAGGTACTTCAAAAAATACCCACCGGATAAAAGAAGAATCCCGCAGAAATATCCCCGGGAGATTCGCAAGCGTAGGGAAGCATTCGAAGAAGCGGTGAATGCAATCAAGATTAACACGCATCTGCTGGCGGAGAAACAGATTGGGAAGATCTTGCGACTGAAGCGGGCCGGATGGGACATAAACGTATTGAATGCGACGGAGGCGTTTAGAGCGGTGGTGAATCCGAAACCCGGGCGGAACCGGCCGGAGATATGGGAGAAGCAGGTGTTGGAGCCAAGTTTGAAAATCGTAAAACGCTTCTTGGAGGAGTAG

Coding sequence (CDS)

ATGTGTGTTGATTCAACATTTTCCTTGAAACTTATGACACTCCCTTCTCCAATTGCTTCTTCTTCTTCCCATTTCTATTCTTCAACTAAAATCTCCCTCCGTTTTCCCTTCTCCCCCAGACCCGCATGGCCTCGCATCAACGCCACTGCCTCCGCCGCTCGCCTTCTTCCTTCCGACGACAAGCTCCTCGTCATTATGGGCGCCACCGGCTGCGGCAAATCCGGCCTCTCCGTCCAGCTCGCCTCTCGTTTTCCCTCTGAAATCATCAACTCCGACAAAATGCAGGTTTATAACGGCCTTGATATTACTACCAACAAGATTCCCCTCCACGAACGAAACGGCGTCCCCCACCACCTTCTTGGCGACTACGATTCTCTCGCCGGCGAATTCACTCCGCTCGACTTCCGAATCCATGCCGGCAATGCCGTCTCCGATATAGCTTCCAGGATGAAACTGCCCATCCTTGTTGGTGGATCCAATTCTTTTATCCATGCAATGCTGGCGAACCATTTCGATCCAAACGACGACGTCTTCGACGGTTCCCGATTCATCTCCTCCGATTTACGGTACCGTTGTTGTTTCCTCTGGCTGGACGTTGCGTTCCCAGTTTTATCAGAATACTTATCGATCAGAGTCGACGACATGCTGGAGGTCGGGATGTTCGAGGAACTAGCTGAGTTTTACAACCCGGACGAGGCCGATACGGCGCCGTATAGCGGGATAAGAAAAGCCATTGGAGTGCCGGAGTTCGACAGGTACTTCAAAAAATACCCACCGGATAAAAGAAGAATCCCGCAGAAATATCCCCGGGAGATTCGCAAGCGTAGGGAAGCATTCGAAGAAGCGGTGAATGCAATCAAGATTAACACGCATCTGCTGGCGGAGAAACAGATTGGGAAGATCTTGCGACTGAAGCGGGCCGGATGGGACATAAACGTATTGAATGCGACGGAGGCGTTTAGAGCGGTGGTGAATCCGAAACCCGGGCGGAACCGGCCGGAGATATGGGAGAAGCAGGTGTTGGAGCCAAGTTTGAAAATCGTAAAACGCTTCTTGGAGGAGTAG

Protein sequence

MCVDSTFSLKLMTLPSPIASSSSHFYSSTKISLRFPFSPRPAWPRINATASAARLLPSDDKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE
Homology
BLAST of Sgr028937 vs. NCBI nr
Match: XP_038893291.1 (adenylate isopentenyltransferase [Benincasa hispida])

HSP 1 Score: 476.5 bits (1225), Expect = 2.0e-130
Identity = 251/359 (69.92%), Postives = 280/359 (77.99%), Query Frame = 0

Query: 1   MCVDSTFSLKLMTLPSPIASSSSHFYSS-TKISLRFPFSPRPAWPRINATASAARLLPSD 60
           M +DST S  LMTLP P +SSSS   SS +   L  PFS R  W R+ A A   +     
Sbjct: 1   MRIDSTSSFNLMTLPPPPSSSSSTSSSSYSSALLNLPFSRRRKWQRVKAIAGGEK----- 60

Query: 61  DKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHL 120
           +K+LVIMGATGCGKSGLSVQLAS F SEIIN DKMQVY GLDITTNKIPLHER+ VPHHL
Sbjct: 61  EKILVIMGATGCGKSGLSVQLASHFQSEIINCDKMQVYKGLDITTNKIPLHERHDVPHHL 120

Query: 121 LGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVF- 180
           LGD DSL  EFTP  FR+ A N VSDI SR KLPILVGGSNSFIHAML NHF+P DDVF 
Sbjct: 121 LGDVDSLHEEFTPFHFRLRADNVVSDICSRNKLPILVGGSNSFIHAMLVNHFNPMDDVFL 180

Query: 181 ----DGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEAD 240
                    ISSDLRYRCCFLWLDV+FP+LSEYLSIRVD+ML++GMFEELAEFY+PD A+
Sbjct: 181 THSHIPKSLISSDLRYRCCFLWLDVSFPILSEYLSIRVDEMLQLGMFEELAEFYHPDTAE 240

Query: 241 TAPYSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLA 300
           T PY+GIRKAIGVPEFD YFKKYPP ++             REAFEEAV+AIK NTH+LA
Sbjct: 241 TTPYTGIRKAIGVPEFDNYFKKYPPGQKN---------GINREAFEEAVDAIKRNTHVLA 300

Query: 301 EKQIGKILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLE 354
           E+QIGKIL+LK AGWDI+VLNATEAFRAVV P  GRNR +IWEKQ+L+PSL+IVKRFLE
Sbjct: 301 ERQIGKILKLKGAGWDIHVLNATEAFRAVVQPGTGRNRKQIWEKQILKPSLRIVKRFLE 345

BLAST of Sgr028937 vs. NCBI nr
Match: XP_022967993.1 (adenylate isopentenyltransferase-like [Cucurbita maxima])

HSP 1 Score: 467.2 bits (1201), Expect = 1.2e-127
Identity = 243/347 (70.03%), Postives = 279/347 (80.40%), Query Frame = 0

Query: 12  MTLPSPIASSSSHFYSSTKIS-LRFPFSPRPAWPR-INATASAARLLPSDDKLLVIMGAT 71
           MTLPS   SSSS+   S++IS L+ P  PR    R +NA A+AA  +   +K+LVI+GAT
Sbjct: 1   MTLPS---SSSSYCSFSSRISFLKAPLIPRRRERRFVNAIAAAATTIGGKEKILVILGAT 60

Query: 72  GCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGE 131
           GCGKSGLSV+L + FP+EIINSDKMQVYNGLDITTNKIPLHER+ VPHH+LGD DS  GE
Sbjct: 61  GCGKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDVDSDKGE 120

Query: 132 FTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS--RFISSD 191
           FTPLDFRI A N VSDIASR KLPILVGGSNSFIHAML  HF+PNDDVF+ +  R ISSD
Sbjct: 121 FTPLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSD 180

Query: 192 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 251
           LRYRCCFLWLDVAFP+L+EYLSIRVD+ML++GMFEELAEFYNP  A+TA YSGIRKAIGV
Sbjct: 181 LRYRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYNPTTAETASYSGIRKAIGV 240

Query: 252 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 311
           PEFD+YFKKYPP          R  +  + AF +AVN IK+NTH+LAE+QIGKIL+LK+A
Sbjct: 241 PEFDKYFKKYPP---------ARNNKIHKSAFNDAVNEIKLNTHVLAERQIGKILKLKQA 300

Query: 312 GWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           GWDINVLNAT AFRA V P    NR EIW+ Q+ EPSL+IV RFLEE
Sbjct: 301 GWDINVLNATAAFRAAVEPGSRMNREEIWKNQIWEPSLRIVNRFLEE 335

BLAST of Sgr028937 vs. NCBI nr
Match: KAG7013020.1 (hypothetical protein SDJN02_25775, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462.6 bits (1189), Expect = 3.0e-126
Identity = 240/347 (69.16%), Postives = 280/347 (80.69%), Query Frame = 0

Query: 12  MTLPSPIASSSSHFYSSTKIS-LRFPFSPRPAWPR-INATASAARLLPSDDKLLVIMGAT 71
           MTLPS  +SSS++   S++IS L+ P  PR    R +NA A+AA      +K+LVI+GAT
Sbjct: 1   MTLPS--SSSSAYCSFSSRISFLKSPLIPRRRERRFVNAIAAAATTTGGKEKILVILGAT 60

Query: 72  GCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGE 131
           GCGKSGLSV+L + FP+EIINSDKMQVYNGLDITTNKIPLHER+ VPHH+LGD DS  GE
Sbjct: 61  GCGKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDIDSDKGE 120

Query: 132 FTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS--RFISSD 191
           FTPLDFRI A N VSDIASR KLPILVGGSNSFIHAML  HF+PNDDVF+ +  R ISSD
Sbjct: 121 FTPLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSD 180

Query: 192 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 251
           LRYRCCFLWLDVAFP+L+EYLSIRVD+ML++GMFEELAEFY+P  A+TA YSGIRKAIGV
Sbjct: 181 LRYRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYDPTTAETASYSGIRKAIGV 240

Query: 252 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 311
           PEFD+YFKKYPP          R  +  + AF++AVN IK+NTH+LA++QIGKIL+LK+A
Sbjct: 241 PEFDKYFKKYPP---------ARNNKIHKSAFDDAVNEIKLNTHVLAKRQIGKILKLKQA 300

Query: 312 GWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           GWDINVLNAT AFRA V P    NR EIW+ Q+ EPSL+IV RFLEE
Sbjct: 301 GWDINVLNATAAFRAAVEPGNRMNREEIWKNQIWEPSLRIVNRFLEE 336

BLAST of Sgr028937 vs. NCBI nr
Match: KAG6573956.1 (hypothetical protein SDJN03_27843, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.1 bits (1185), Expect = 8.8e-126
Identity = 240/347 (69.16%), Postives = 278/347 (80.12%), Query Frame = 0

Query: 12  MTLPSPIASSSSHFYSSTKIS-LRFPFSPRPAWPR-INATASAARLLPSDDKLLVIMGAT 71
           MTLPS   SS S+   S++IS L+ P  PR    R +NA A+AA      +K+LVI+GAT
Sbjct: 1   MTLPS---SSCSYCSFSSRISFLKSPLIPRRRERRFVNAIAAAATTTGGKEKILVILGAT 60

Query: 72  GCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGE 131
           GCGKSGLSV+L + FP+EIINSDKMQVYNGLDITTNKIPLHER+ VPHH+LGD DS  GE
Sbjct: 61  GCGKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDIDSDKGE 120

Query: 132 FTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS--RFISSD 191
           FTPLDFRI A N VSDIASR KLPILVGGSNSFIHAML  HF+PNDDVF+ +  R ISSD
Sbjct: 121 FTPLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSD 180

Query: 192 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 251
           LRYRCCFLWLDVAFP+L+EYLSIRVD+ML++GMFEELAEFY+P  A+TA YSGIRKAIGV
Sbjct: 181 LRYRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYDPTTAETASYSGIRKAIGV 240

Query: 252 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 311
           PEFD+YFKKYPP          R  +  + AF++AVN IK+NTH+LA++QIGKIL+LK+A
Sbjct: 241 PEFDKYFKKYPP---------ARNNKIHKSAFDDAVNEIKLNTHVLAKRQIGKILKLKQA 300

Query: 312 GWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           GWDINVLNAT AFRA V P    NR EIW+ Q+ EPSL+IV RFLEE
Sbjct: 301 GWDINVLNATAAFRAAVEPGNRMNREEIWKNQIWEPSLRIVNRFLEE 335

BLAST of Sgr028937 vs. NCBI nr
Match: XP_031741216.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN52088.1 hypothetical protein Csa_008393 [Cucumis sativus])

HSP 1 Score: 459.9 bits (1182), Expect = 2.0e-125
Identity = 244/360 (67.78%), Postives = 273/360 (75.83%), Query Frame = 0

Query: 1   MCVDSTFSLKLMTLPSPIASSSSHFYSSTKIS------LRFPFSPRPAWPRINATASAAR 60
           M V S  S  LMTL S   SSSS   SS+  S      L  PFS R    ++ A A+AA 
Sbjct: 1   MRVGSASSFNLMTLSSSSYSSSSSSSSSSSSSSSKIFILNIPFSRRKKRQQVKAIAAAA- 60

Query: 61  LLPSDDKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNG 120
               D  +LVIMGATGCGKSGLSVQLAS + SEIIN DKMQVY GLDITTNKIPLHER+ 
Sbjct: 61  TTGKDQNILVIMGATGCGKSGLSVQLASHYQSEIINCDKMQVYKGLDITTNKIPLHERHD 120

Query: 121 VPHHLLGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPN 180
           VPHHLLGD DS+  +FTP  FR+ A N VSDIASR KLPILVGGSNSFIHAML NHF+PN
Sbjct: 121 VPHHLLGDVDSIHQDFTPFHFRLRADNVVSDIASRNKLPILVGGSNSFIHAMLVNHFNPN 180

Query: 181 DDVFDGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEAD 240
            DVF     ISS LRYRCCFLWLDV FP+L+EYLSIRVD+ML++GMFEELAEFY+P  A+
Sbjct: 181 HDVFTPKSLISSHLRYRCCFLWLDVEFPILAEYLSIRVDEMLQIGMFEELAEFYDPHTAE 240

Query: 241 TAPYSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLA 300
           T PY+GIRKAIGVPEFD+YF+K PP K+             REAFEEAV AIK NTH+LA
Sbjct: 241 TMPYTGIRKAIGVPEFDKYFQKNPPSKKS---------GIDREAFEEAVEAIKRNTHVLA 300

Query: 301 EKQIGKILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           E+QIGKIL+LK AGWDINVLNATEAFRAVV P  GRNR EIWEK++LEPS +IV RFL+E
Sbjct: 301 ERQIGKILKLKEAGWDINVLNATEAFRAVVEPGTGRNRKEIWEKEILEPSFRIVNRFLKE 350

BLAST of Sgr028937 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.4e-97
Identity = 189/302 (62.58%), Postives = 230/302 (76.16%), Query Frame = 0

Query: 60  DKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHL 119
           +KLLV+MGATG GKS LS+ LA+ FP E+INSDKMQVY GLDITTNKI + +R GVPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 120 LGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPN-DDVF 179
           LG+ D   GE TP DFR  AG AVS+I  R KLP+LVGGSNSFIHA+L + FD +   VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 180 -DGSR-FISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPD----E 239
            +GS   +SS+LRY CCFLW+DV+  VL++YL+ RVDDMLE+GMF+ELAEFY+P+    +
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 240 ADTAPYSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHL 299
            D+A  +G+RKAIGVPEFDRYF+K+ P    +  + P   R RR AFEEAV AIK NT  
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGD--VEGEDPGRDRVRRGAFEEAVRAIKENTCH 269

Query: 300 LAEKQIGKILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFL 355
           LA++QIGKILRLK AGWD+  L+ATE+FRA +    G    EIWEKQVLEPS+KIV RFL
Sbjct: 270 LAKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFL 329

BLAST of Sgr028937 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 320.9 bits (821), Expect = 1.9e-86
Identity = 180/346 (52.02%), Postives = 234/346 (67.63%), Query Frame = 0

Query: 10  KLMTLPSPIASSSSHFYSSTKISLRFPFSPRPAWPRINA-TASAARLLPSDDKLLVIMGA 69
           +  T  +   S SSH  SS+ + L F    R   P +++     +R     DK++VI+GA
Sbjct: 15  RFTTTTTTSPSFSSHSSSSSSL-LSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGA 74

Query: 70  TGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAG 129
           TG GKS LSV LA+RFPSEIINSDK+QVY GL+ITTN+I L +R GVPHHLLG  +   G
Sbjct: 75  TGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHG 134

Query: 130 EFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVF-DGSRFISSD 189
           E T  +FR  A N V +I SR K+PI+ GGSNSF+HA+LA  FDP  D F  GS  ISSD
Sbjct: 135 ELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSD 194

Query: 190 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 249
           LRY CCF+W+DV+  VL EYL  RVD+M++ GMFEEL+ FY+P ++      GIRKAIGV
Sbjct: 195 LRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGV 254

Query: 250 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 309
           PEFD YFK+YPP+K+ I     +    R+ A+++AV+ IK NT  LA++Q+ KI  LK A
Sbjct: 255 PEFDGYFKEYPPEKKMI-----KWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA 314

Query: 310 GWDINVLNATEAFRAVVNPKPGRNR-PEIWEKQVLEPSLKIVKRFL 353
           GW+I  ++AT +F+AV+       +  E WE+QVLEPS+KIVKR L
Sbjct: 315 GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Sgr028937 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.9e-79
Identity = 156/293 (53.24%), Postives = 203/293 (69.28%), Query Frame = 0

Query: 61  KLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLL 120
           K++VIMGATG GKS LS+ LA+RF  EI+NSDK+Q Y+GL +TTN++ + ER GVPHHLL
Sbjct: 44  KVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLL 103

Query: 121 GDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDG 180
           G+      E T  +FR  A  ++S+I +R  LPI+ GGSNSFIHA+L + FDP    F  
Sbjct: 104 GELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSS 163

Query: 181 SRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYS- 240
              ISS LRY CCFLW+DV+  VL EYLS RVD M+E GMFEELA FY+P  + +A  + 
Sbjct: 164 ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAH 223

Query: 241 GIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIG 300
           GI K IG+PEFDRYF  YPP+++   QK     + R+ A++EAV  IK NT  LA+KQI 
Sbjct: 224 GIHKTIGIPEFDRYFSLYPPERK---QKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIE 283

Query: 301 KILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFL 353
           +I++LK +GWDI  L+AT +F        GR+  EIW+  VL+ S+K+VKRFL
Sbjct: 284 RIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Sgr028937 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.3e-75
Identity = 158/306 (51.63%), Postives = 205/306 (66.99%), Query Frame = 0

Query: 59  DDKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHH 118
           +DK++VIMGATG GKS LSV LA  F +EIINSDKMQ Y+GL ITTN+  + +R GVPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 119 LLGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVF 178
           LLG+ +  AGE T  +FR+ A  A+S+I  R KLPIL GGSNS+IHA+LA  +DP +  F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 179 -DGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAP 238
            D    I S+L+Y CCF+W+DV   VL EYLS+R+D M++ GMFEE+AEF+   +A   P
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 239 YSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRK----RREAFEEAVNAIKINTHLL 298
             GI KAIGV EFD Y K Y         K+  ++ K    R+EA+E+AV AIK NT  L
Sbjct: 184 L-GIWKAIGVQEFDDYLKMY---------KWDNDMDKWDPMRKEAYEKAVRAIKENTFQL 243

Query: 299 AEKQIGKILRLKRAGWDINVLNATEAFR-AVVNPKPGRNRPE----IWEKQVLEPSLKIV 355
            + QI KI +L+ AGWDI  ++AT +FR A+   K G    E    IW K+VLEP +KIV
Sbjct: 244 TKDQITKINKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIV 299

BLAST of Sgr028937 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 170/353 (48.16%), Postives = 221/353 (62.61%), Query Frame = 0

Query: 10  KLMTLPSPIASSSSHFYSSTKISLRFPFSPRPAWPRINATASAARLLPSDDKLLVIMGAT 69
           +LMTL SP  S SS       ++ +F  SPR     +      A      DK+++I G T
Sbjct: 3   QLMTLLSPPLSHSSLL---PTVTTKFG-SPR----LVTTCMGHAGRKNIKDKVVLITGTT 62

Query: 70  GCGKSGLSVQLASR-FPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAG 129
           G GKS LSV LA+R FP+EIINSDKMQ+Y G +I TN IPLHE+ GVPHHLLG +    G
Sbjct: 63  GTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDG 122

Query: 130 EFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFD-GSRF--IS 189
           E TP +FR  A  ++S + S  KLPI+VGGSNSF HA+LA  FDP+ D F  GS    I 
Sbjct: 123 ELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTIC 182

Query: 190 SDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAI 249
           SDLRY+CC LW+DV  PVL ++L  RVD M+E G+ E+LAE Y+P   D+    G+RK I
Sbjct: 183 SDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTI 242

Query: 250 GVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLK 309
           GV EFDRYF+ YP +      K   ++  R+ A+EE V  +K  T  L +KQ  KI++L 
Sbjct: 243 GVEEFDRYFRVYPKEM----DKGIWDL-ARKAAYEETVKGMKERTCRLVKKQKEKIMKLI 302

Query: 310 RAGWDINVLNATEAFRAVVNPK----PGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           R GW+I  L+AT A  A +N       G+N  EIWEK +++ S++IVK+FL E
Sbjct: 303 RGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Sgr028937 vs. ExPASy TrEMBL
Match: A0A6J1HWS1 (adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111467363 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.9e-128
Identity = 243/347 (70.03%), Postives = 279/347 (80.40%), Query Frame = 0

Query: 12  MTLPSPIASSSSHFYSSTKIS-LRFPFSPRPAWPR-INATASAARLLPSDDKLLVIMGAT 71
           MTLPS   SSSS+   S++IS L+ P  PR    R +NA A+AA  +   +K+LVI+GAT
Sbjct: 1   MTLPS---SSSSYCSFSSRISFLKAPLIPRRRERRFVNAIAAAATTIGGKEKILVILGAT 60

Query: 72  GCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGE 131
           GCGKSGLSV+L + FP+EIINSDKMQVYNGLDITTNKIPLHER+ VPHH+LGD DS  GE
Sbjct: 61  GCGKSGLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDVDSDKGE 120

Query: 132 FTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS--RFISSD 191
           FTPLDFRI A N VSDIASR KLPILVGGSNSFIHAML  HF+PNDDVF+ +  R ISSD
Sbjct: 121 FTPLDFRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSD 180

Query: 192 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 251
           LRYRCCFLWLDVAFP+L+EYLSIRVD+ML++GMFEELAEFYNP  A+TA YSGIRKAIGV
Sbjct: 181 LRYRCCFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYNPTTAETASYSGIRKAIGV 240

Query: 252 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 311
           PEFD+YFKKYPP          R  +  + AF +AVN IK+NTH+LAE+QIGKIL+LK+A
Sbjct: 241 PEFDKYFKKYPP---------ARNNKIHKSAFNDAVNEIKLNTHVLAERQIGKILKLKQA 300

Query: 312 GWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           GWDINVLNAT AFRA V P    NR EIW+ Q+ EPSL+IV RFLEE
Sbjct: 301 GWDINVLNATAAFRAAVEPGSRMNREEIWKNQIWEPSLRIVNRFLEE 335

BLAST of Sgr028937 vs. ExPASy TrEMBL
Match: A0A0A0KR70 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609740 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 9.5e-126
Identity = 244/360 (67.78%), Postives = 273/360 (75.83%), Query Frame = 0

Query: 1   MCVDSTFSLKLMTLPSPIASSSSHFYSSTKIS------LRFPFSPRPAWPRINATASAAR 60
           M V S  S  LMTL S   SSSS   SS+  S      L  PFS R    ++ A A+AA 
Sbjct: 1   MRVGSASSFNLMTLSSSSYSSSSSSSSSSSSSSSKIFILNIPFSRRKKRQQVKAIAAAA- 60

Query: 61  LLPSDDKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNG 120
               D  +LVIMGATGCGKSGLSVQLAS + SEIIN DKMQVY GLDITTNKIPLHER+ 
Sbjct: 61  TTGKDQNILVIMGATGCGKSGLSVQLASHYQSEIINCDKMQVYKGLDITTNKIPLHERHD 120

Query: 121 VPHHLLGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPN 180
           VPHHLLGD DS+  +FTP  FR+ A N VSDIASR KLPILVGGSNSFIHAML NHF+PN
Sbjct: 121 VPHHLLGDVDSIHQDFTPFHFRLRADNVVSDIASRNKLPILVGGSNSFIHAMLVNHFNPN 180

Query: 181 DDVFDGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEAD 240
            DVF     ISS LRYRCCFLWLDV FP+L+EYLSIRVD+ML++GMFEELAEFY+P  A+
Sbjct: 181 HDVFTPKSLISSHLRYRCCFLWLDVEFPILAEYLSIRVDEMLQIGMFEELAEFYDPHTAE 240

Query: 241 TAPYSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLA 300
           T PY+GIRKAIGVPEFD+YF+K PP K+             REAFEEAV AIK NTH+LA
Sbjct: 241 TMPYTGIRKAIGVPEFDKYFQKNPPSKKS---------GIDREAFEEAVEAIKRNTHVLA 300

Query: 301 EKQIGKILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           E+QIGKIL+LK AGWDINVLNATEAFRAVV P  GRNR EIWEK++LEPS +IV RFL+E
Sbjct: 301 ERQIGKILKLKEAGWDINVLNATEAFRAVVEPGTGRNRKEIWEKEILEPSFRIVNRFLKE 350

BLAST of Sgr028937 vs. ExPASy TrEMBL
Match: A0A6J1G1X1 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449909 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.1e-124
Identity = 233/342 (68.13%), Postives = 274/342 (80.12%), Query Frame = 0

Query: 17  PIASSSSHFYSSTKIS-LRFPFSPRPAWPR-INATASAARLLPSDDKLLVIMGATGCGKS 76
           P   ++++   S++IS L+ P  PR    R +NA A+AA      +K+LVI+GATGCGKS
Sbjct: 26  PHGHNAAYCSFSSRISFLKSPLIPRRRERRFVNAIAAAATTTGGKEKILVILGATGCGKS 85

Query: 77  GLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGEFTPLD 136
           GLSV+L + FP+EIINSDKMQVYNGLDITTNKIPLHER+ VPHH+LGD DS  GEFTPLD
Sbjct: 86  GLSVRLGTHFPAEIINSDKMQVYNGLDITTNKIPLHERHDVPHHILGDIDSDKGEFTPLD 145

Query: 137 FRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS--RFISSDLRYRC 196
           FRI A N VSDIASR KLPILVGGSNSFIHAML  HF+PNDDVF+ +  R ISSDLRYRC
Sbjct: 146 FRIRADNVVSDIASRSKLPILVGGSNSFIHAMLVTHFNPNDDVFNATIPRLISSDLRYRC 205

Query: 197 CFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGVPEFDR 256
           CFLWLDVAFP+L+EYLSIRVD+ML++GMFEELAEFY+P  A+TA YSGIRKAIGVPEFD+
Sbjct: 206 CFLWLDVAFPILAEYLSIRVDEMLQLGMFEELAEFYDPTTAETASYSGIRKAIGVPEFDK 265

Query: 257 YFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRAGWDIN 316
           YFKKYPP          R  +  + AF++AVN IK+NTH+LA++QIGKIL+LK+AGWDIN
Sbjct: 266 YFKKYPP---------ARNNKIHKSAFDDAVNEIKLNTHVLAKRQIGKILKLKQAGWDIN 325

Query: 317 VLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           VLNAT AFRA V P    NR EIW+ Q+ EPSL+IV RFLEE
Sbjct: 326 VLNATAAFRAAVEPGNRMNREEIWKNQIWEPSLRIVNRFLEE 358

BLAST of Sgr028937 vs. ExPASy TrEMBL
Match: A0A1S4DW42 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990777 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.5e-123
Identity = 239/340 (70.29%), Postives = 268/340 (78.82%), Query Frame = 0

Query: 19  ASSSSHFYSSTKI-SLRFPFSPRPAWPRINATASAARLLPSDDKLLVIMGATGCGKSGLS 78
           +SSSS  YSSTKI  L  PFS R    ++ A A+AA      +K+LVIMGATGCGKSGLS
Sbjct: 3   SSSSSSSYSSTKIFILNIPFSRRRKRHQVKAIAAAA--AAGKEKILVIMGATGCGKSGLS 62

Query: 79  VQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAGEFTPLDFRI 138
           VQLAS + +EIIN DKMQVY GLDITTNKIPLHER+ VPHHLLGD DS+  +FTP  FR 
Sbjct: 63  VQLASHYQAEIINCDKMQVYKGLDITTNKIPLHERHDVPHHLLGDVDSIHEDFTPFHFRP 122

Query: 139 HAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDG---SRFISSDLRYRCCF 198
            A N VS+IASR KLPILVGGSNSFIHAML N F+PN DVF        ISSDLRYRCCF
Sbjct: 123 RADNVVSNIASRNKLPILVGGSNSFIHAMLVNRFNPNHDVFTNCIPKPLISSDLRYRCCF 182

Query: 199 LWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGVPEFDRYF 258
           LWLDVAFP+LSEYLSIRVD+ML++GMFEELAEFY+P  A+T PY+GIRKAIGVPEFD+YF
Sbjct: 183 LWLDVAFPILSEYLSIRVDEMLQLGMFEELAEFYDPHTAETMPYTGIRKAIGVPEFDKYF 242

Query: 259 KKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRAGWDINVL 318
           KK PP K+             REAFEEAV AIK NTH+LAE+QIGKIL+LK AGWDINVL
Sbjct: 243 KKNPPSKKS---------GIDREAFEEAVEAIKRNTHVLAERQIGKILKLKEAGWDINVL 302

Query: 319 NATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           NATEAFRAVV P  GRNR EIWEK++LEPS +IV RFL+E
Sbjct: 303 NATEAFRAVVEPGIGRNRKEIWEKEILEPSFRIVNRFLKE 331

BLAST of Sgr028937 vs. ExPASy TrEMBL
Match: A0A2I4DWG2 (adenylate isopentenyltransferase-like isoform X1 OS=Juglans regia OX=51240 GN=LOC108984109 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 4.6e-104
Identity = 205/356 (57.58%), Postives = 262/356 (73.60%), Query Frame = 0

Query: 12  MTLPSPIASSSSHFYSSTKISLRFPFSP-----RPAWPRI-----NATASAARLLPSDDK 71
           MTL  P  SS SH   S+++   F FSP     RP WPR+     N T + AR     +K
Sbjct: 1   MTL-HPFQSSISHSCYSSRVLSFFFFSPASKPLRPCWPRMDSAAANKTIATARRQRKKEK 60

Query: 72  LLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLG 131
           ++V++GATG GK+ LSV+LA+RF +EI+NSDKMQVY GLDITTNKIP HER G+ HHL+G
Sbjct: 61  IVVVLGATGAGKTRLSVELATRFQAEIVNSDKMQVYKGLDITTNKIPPHERRGIAHHLMG 120

Query: 132 DYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDGS 191
           ++D + GEFTP +FR  AG+ +SDIASR KLP++VGGSNSF+HA++ + F+P  D+FDGS
Sbjct: 121 EFDPVDGEFTPAEFRHLAGSVISDIASRKKLPLVVGGSNSFVHALVVDRFNPQIDIFDGS 180

Query: 192 RFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDE-ADTA-PYS 251
             +SS+LRY CCFLW+DV+F VLSEYLS RVD+ML+ GMF+ELAEFYNP E AD+A P +
Sbjct: 181 HSLSSELRYNCCFLWVDVSFTVLSEYLSKRVDEMLDSGMFQELAEFYNPTESADSAGPRT 240

Query: 252 GIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIG 311
           G+RKAIGVPEFD YFKK+PPD     Q        +  A+EEAV  IK NT  LA++QIG
Sbjct: 241 GLRKAIGVPEFDCYFKKHPPD-----QSVDGADHGQTAAYEEAVREIKENTCQLAKRQIG 300

Query: 312 KILRLKRAGWDINVLNATEAFRAVVNPKP-GRNRPEIWEKQVLEPSLKIVKRFLEE 355
           KI+ L+  GWD+  L+ATE FRA + P+  G+   EIW+++VLEPS+KIVK FLEE
Sbjct: 301 KIVGLRGGGWDLRRLDATEPFRAAMKPEGCGKRWSEIWQREVLEPSVKIVKHFLEE 350

BLAST of Sgr028937 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-87
Identity = 180/346 (52.02%), Postives = 234/346 (67.63%), Query Frame = 0

Query: 10  KLMTLPSPIASSSSHFYSSTKISLRFPFSPRPAWPRINA-TASAARLLPSDDKLLVIMGA 69
           +  T  +   S SSH  SS+ + L F    R   P +++     +R     DK++VI+GA
Sbjct: 15  RFTTTTTTSPSFSSHSSSSSSL-LSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGA 74

Query: 70  TGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAG 129
           TG GKS LSV LA+RFPSEIINSDK+QVY GL+ITTN+I L +R GVPHHLLG  +   G
Sbjct: 75  TGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHG 134

Query: 130 EFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVF-DGSRFISSD 189
           E T  +FR  A N V +I SR K+PI+ GGSNSF+HA+LA  FDP  D F  GS  ISSD
Sbjct: 135 ELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSD 194

Query: 190 LRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAIGV 249
           LRY CCF+W+DV+  VL EYL  RVD+M++ GMFEEL+ FY+P ++      GIRKAIGV
Sbjct: 195 LRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGV 254

Query: 250 PEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLKRA 309
           PEFD YFK+YPP+K+ I     +    R+ A+++AV+ IK NT  LA++Q+ KI  LK A
Sbjct: 255 PEFDGYFKEYPPEKKMI-----KWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA 314

Query: 310 GWDINVLNATEAFRAVVNPKPGRNR-PEIWEKQVLEPSLKIVKRFL 353
           GW+I  ++AT +F+AV+       +  E WE+QVLEPS+KIVKR L
Sbjct: 315 GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Sgr028937 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 296.2 bits (757), Expect = 3.5e-80
Identity = 156/293 (53.24%), Postives = 203/293 (69.28%), Query Frame = 0

Query: 61  KLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLL 120
           K++VIMGATG GKS LS+ LA+RF  EI+NSDK+Q Y+GL +TTN++ + ER GVPHHLL
Sbjct: 44  KVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLL 103

Query: 121 GDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFDG 180
           G+      E T  +FR  A  ++S+I +R  LPI+ GGSNSFIHA+L + FDP    F  
Sbjct: 104 GELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSS 163

Query: 181 SRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYS- 240
              ISS LRY CCFLW+DV+  VL EYLS RVD M+E GMFEELA FY+P  + +A  + 
Sbjct: 164 ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAH 223

Query: 241 GIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIG 300
           GI K IG+PEFDRYF  YPP+++   QK     + R+ A++EAV  IK NT  LA+KQI 
Sbjct: 224 GIHKTIGIPEFDRYFSLYPPERK---QKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIE 283

Query: 301 KILRLKRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFL 353
           +I++LK +GWDI  L+AT +F        GR+  EIW+  VL+ S+K+VKRFL
Sbjct: 284 RIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Sgr028937 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-76
Identity = 158/306 (51.63%), Postives = 205/306 (66.99%), Query Frame = 0

Query: 59  DDKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHH 118
           +DK++VIMGATG GKS LSV LA  F +EIINSDKMQ Y+GL ITTN+  + +R GVPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 119 LLGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVF 178
           LLG+ +  AGE T  +FR+ A  A+S+I  R KLPIL GGSNS+IHA+LA  +DP +  F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 179 -DGSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAP 238
            D    I S+L+Y CCF+W+DV   VL EYLS+R+D M++ GMFEE+AEF+   +A   P
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 239 YSGIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRK----RREAFEEAVNAIKINTHLL 298
             GI KAIGV EFD Y K Y         K+  ++ K    R+EA+E+AV AIK NT  L
Sbjct: 184 L-GIWKAIGVQEFDDYLKMY---------KWDNDMDKWDPMRKEAYEKAVRAIKENTFQL 243

Query: 299 AEKQIGKILRLKRAGWDINVLNATEAFR-AVVNPKPGRNRPE----IWEKQVLEPSLKIV 355
            + QI KI +L+ AGWDI  ++AT +FR A+   K G    E    IW K+VLEP +KIV
Sbjct: 244 TKDQITKINKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIV 299

BLAST of Sgr028937 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 278.5 bits (711), Expect = 7.5e-75
Identity = 170/353 (48.16%), Postives = 221/353 (62.61%), Query Frame = 0

Query: 10  KLMTLPSPIASSSSHFYSSTKISLRFPFSPRPAWPRINATASAARLLPSDDKLLVIMGAT 69
           +LMTL SP  S SS       ++ +F  SPR     +      A      DK+++I G T
Sbjct: 3   QLMTLLSPPLSHSSLL---PTVTTKFG-SPR----LVTTCMGHAGRKNIKDKVVLITGTT 62

Query: 70  GCGKSGLSVQLASR-FPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHLLGDYDSLAG 129
           G GKS LSV LA+R FP+EIINSDKMQ+Y G +I TN IPLHE+ GVPHHLLG +    G
Sbjct: 63  GTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDG 122

Query: 130 EFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFD-GSRF--IS 189
           E TP +FR  A  ++S + S  KLPI+VGGSNSF HA+LA  FDP+ D F  GS    I 
Sbjct: 123 ELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTIC 182

Query: 190 SDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYSGIRKAI 249
           SDLRY+CC LW+DV  PVL ++L  RVD M+E G+ E+LAE Y+P   D+    G+RK I
Sbjct: 183 SDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTI 242

Query: 250 GVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIGKILRLK 309
           GV EFDRYF+ YP +      K   ++  R+ A+EE V  +K  T  L +KQ  KI++L 
Sbjct: 243 GVEEFDRYFRVYPKEM----DKGIWDL-ARKAAYEETVKGMKERTCRLVKKQKEKIMKLI 302

Query: 310 RAGWDINVLNATEAFRAVVNPK----PGRNRPEIWEKQVLEPSLKIVKRFLEE 355
           R GW+I  L+AT A  A +N       G+N  EIWEK +++ S++IVK+FL E
Sbjct: 303 RGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Sgr028937 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 220.7 bits (561), Expect = 1.9e-57
Identity = 127/294 (43.20%), Postives = 178/294 (60.54%), Query Frame = 0

Query: 60  DKLLVIMGATGCGKSGLSVQLASRFPSEIINSDKMQVYNGLDITTNKIPLHERNGVPHHL 119
           DK++ +MGATG GKS L++ LA+RFP+EI+NSDK+QVY GLDI TNK+   E  GVPHHL
Sbjct: 33  DKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHL 92

Query: 120 LGDYDSLAGEFTPLDFRIHAGNAVSDIASRMKLPILVGGSNSFIHAMLANHFDPNDDVFD 179
           LG       +FT  DF+  A  AV  I  R ++PI+ GGSNS+I A++      ND    
Sbjct: 93  LGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV------ND---- 152

Query: 180 GSRFISSDLRYRCCFLWLDVAFPVLSEYLSIRVDDMLEVGMFEELAEFYNPDEADTAPYS 239
               +   LRY CCFLW+DV+ PVL  ++S RVD M+++G+ +E+   ++P  +D +  +
Sbjct: 153 ---CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYS--A 212

Query: 240 GIRKAIGVPEFDRYFKKYPPDKRRIPQKYPREIRKRREAFEEAVNAIKINTHLLAEKQIG 299
           GIR+AIGVPE D +        R   + YP E  +R    E A+  IK NT LLA +Q+ 
Sbjct: 213 GIRRAIGVPELDEFL-------RSEMRNYPAETTER--LLETAIEKIKENTCLLACRQLQ 272

Query: 300 KILRL-KRAGWDINVLNATEAFRAVVNPKPGRNRPEIWEKQVLEPSLKIVKRFL 353
           KI RL K+  W+++ ++ATE F      + G    E W+  V  PS   V++FL
Sbjct: 273 KIQRLYKQWKWNMHRVDATEVFL-----RRGEEADEAWDNSVAHPSALAVEKFL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893291.12.0e-13069.92adenylate isopentenyltransferase [Benincasa hispida][more]
XP_022967993.11.2e-12770.03adenylate isopentenyltransferase-like [Cucurbita maxima][more]
KAG7013020.13.0e-12669.16hypothetical protein SDJN02_25775, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6573956.18.8e-12669.16hypothetical protein SDJN03_27843, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_031741216.12.0e-12567.78adenylate isopentenyltransferase [Cucumis sativus] >KGN52088.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
Q5GHF71.4e-9762.58Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.9e-8652.02Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9LJL44.9e-7953.24Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9SB603.3e-7551.63Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ... [more]
Q9C6L11.1e-7348.16Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1HWS15.9e-12870.03adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11146736... [more]
A0A0A0KR709.5e-12667.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G609740 PE=4 SV=1[more]
A0A6J1G1X13.1e-12468.13adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449... [more]
A0A1S4DW421.5e-12370.29adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990777 PE... [more]
A0A2I4DWG24.6e-10457.58adenylate isopentenyltransferase-like isoform X1 OS=Juglans regia OX=51240 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G68460.11.3e-8752.02isopentenyltransferase 1 [more]
AT3G19160.13.5e-8053.24ATP/ADP isopentenyltransferases [more]
AT4G24650.12.3e-7651.63isopentenyltransferase 4 [more]
AT1G25410.17.5e-7548.16isopentenyltransferase 6 [more]
AT5G19040.11.9e-5743.20isopentenyltransferase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 188..298
e-value: 5.1E-9
score: 36.2
coord: 95..170
e-value: 1.3E-16
score: 61.1
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 201..311
e-value: 2.2E-122
score: 409.4
NoneNo IPR availablePANTHERPTHR11088:SF65ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATEDcoord: 58..353
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 60..352
e-value: 2.2E-122
score: 409.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 61..235
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 58..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr028937.1Sgr028937.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0052381 tRNA dimethylallyltransferase activity