Sgr028836 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028836 vs. NCBI nr
Match: XP_022139206.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Momordica charantia])

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-35
Identity = 93/155 (60.00%), Postives = 108/155 (69.68%), Query Frame = 0

Query: 1   MVPRQPSPLLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLV 60
           M+P+ P PLL LLHLL F+ AAA                  +FQ+LNVKE KFD +DD +
Sbjct: 1   MLPKIPFPLLFLLHLLIFSAAAA-----------------TDFQFLNVKETKFDSNDDRI 60

Query: 61  FHNLDFSDDGHRLVMGGKTNSS---KFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRV 120
            HNL+FS DGHR V G K+NSS   KFKLKLVHRDKLP SHVHG RHGF +RMKRDA RV
Sbjct: 61  LHNLNFS-DGHRQVTGKKSNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRV 120

Query: 121 ASLIRRLSNGGAATTPAEQGNSFKVANFATDVISG 153
           A+L+RRLS+GGA        +S+K ANFATDVISG
Sbjct: 121 AALVRRLSHGGA-------DSSYKAANFATDVISG 130

BLAST of Sgr028836 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 132.9 bits (333), Expect = 2.4e-27
Identity = 81/152 (53.29%), Postives = 102/152 (67.11%), Query Frame = 0

Query: 1   MVPRQPSPLLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLV 60
           M  +QPS LL  L  LFF  +AAA  ++P+            FQYLNVK  K D +DD +
Sbjct: 1   MFLKQPSSLLFFLFFLFF--SAAAGRSSPT-----------KFQYLNVKATKLDFNDDQI 60

Query: 61  FHNLDFSDDGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASL 120
            H L+FS    R V G K++++ FKL L+HRDK  LSHVHGHRHGF++R+KRDA RVA+L
Sbjct: 61  LHTLNFS-HARRQVSGHKSDNNTFKLNLLHRDK--LSHVHGHRHGFNERIKRDAIRVATL 120

Query: 121 IRRLSNGGAATTPAEQGNSFKVANFATDVISG 153
           +RRLS+GG A     Q N +KVA+FATDVISG
Sbjct: 121 VRRLSHGGGAV----QDNKYKVADFATDVISG 132

BLAST of Sgr028836 vs. NCBI nr
Match: XP_004147103.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothetical protein Csa_008597 [Cucumis sativus])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 81/144 (56.25%), Postives = 98/144 (68.06%), Query Frame = 0

Query: 9   LLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSD 68
           L S L  L   +  AAAAA  SSS+         FQYLNVK  K D +D  + H L+FS 
Sbjct: 6   LFSSLLFLLVVIVGAAAAARLSSST--------KFQYLNVKATKLDFNDGQILHALNFS- 65

Query: 69  DGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGG 128
           DGHR V G K++++ FKL L+HRDK  LSHVHGHR GF+DRMKRDA RVA+L+RRLS+G 
Sbjct: 66  DGHRQVSGYKSDNNTFKLNLLHRDK--LSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA 125

Query: 129 AATTPAEQGNSFKVANFATDVISG 153
            A   A + + +KVANFATDVISG
Sbjct: 126 PA---AVKDSRYKVANFATDVISG 135

BLAST of Sgr028836 vs. NCBI nr
Match: XP_008445900.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 79/144 (54.86%), Postives = 95/144 (65.97%), Query Frame = 0

Query: 9   LLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSD 68
           L+S L      +   AAAA PSS +         FQYLNVK  K D +D  + H L+FS 
Sbjct: 6   LISSLLFFLAVLVVVAAAARPSSPT--------KFQYLNVKATKLDFNDGQILHTLNFS- 65

Query: 69  DGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGG 128
           D HR V G K+++  FKL L+HRDK  LSHVHGHR GF+DRMKRDA RVA+L+RRLS+G 
Sbjct: 66  DAHRQVSGHKSHNDTFKLNLLHRDK--LSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA 125

Query: 129 AATTPAEQGNSFKVANFATDVISG 153
           AA     + + FKVANFATDVISG
Sbjct: 126 AAV----EDSRFKVANFATDVISG 134

BLAST of Sgr028836 vs. NCBI nr
Match: XP_022966851.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita maxima])

HSP 1 Score: 126.3 bits (316), Expect = 2.3e-25
Identity = 76/138 (55.07%), Postives = 92/138 (66.67%), Query Frame = 0

Query: 15  LLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSDDGHRLV 74
           LLFF+V+           S SSS     FQYLNVK  KFD +D  + H L+FS +GHRLV
Sbjct: 10  LLFFSVSVVL-------GSRSSS---VEFQYLNVKSNKFDVNDSQLLHTLNFS-NGHRLV 69

Query: 75  MGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGGAATTPA 134
            G K+N +KFKLKLVHRDKLP  H+HG   GF +R+KRD +RVA+L+RRLS     T+  
Sbjct: 70  TGRKSNHTKFKLKLVHRDKLP--HIHGQHQGFDERIKRDVKRVATLVRRLSRDDTTTSVV 129

Query: 135 EQGNSFKVANFATDVISG 153
           E  NS+KVANF T VISG
Sbjct: 130 ED-NSYKVANFGTGVISG 133

BLAST of Sgr028836 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-08
Identity = 40/121 (33.06%), Postives = 66/121 (54.55%), Query Frame = 0

Query: 33  STSSSRHHANFQYLNVKEIKFDPDDDLV-FHNLDFSDDGHRLVMGGKTNSSKFKLKLVHR 92
           S+SSS    +FQ ++V +        L  F+N  FSD+          +SSK+ L+L+HR
Sbjct: 17  SSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE----------SSSKYTLRLLHR 76

Query: 93  DKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGGAATTPAEQGNSFKVANFATDVIS 152
           D+ P      H H  H RM+RD  RV++++RR+S       P+   + ++V +F +D++S
Sbjct: 77  DRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---GKVIPSSD-SRYEVNDFGSDIVS 123

BLAST of Sgr028836 vs. ExPASy TrEMBL
Match: A0A6J1CBZ9 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Momordica charantia OX=3673 GN=LOC111010176 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 5.2e-36
Identity = 93/155 (60.00%), Postives = 108/155 (69.68%), Query Frame = 0

Query: 1   MVPRQPSPLLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLV 60
           M+P+ P PLL LLHLL F+ AAA                  +FQ+LNVKE KFD +DD +
Sbjct: 1   MLPKIPFPLLFLLHLLIFSAAAA-----------------TDFQFLNVKETKFDSNDDRI 60

Query: 61  FHNLDFSDDGHRLVMGGKTNSS---KFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRV 120
            HNL+FS DGHR V G K+NSS   KFKLKLVHRDKLP SHVHG RHGF +RMKRDA RV
Sbjct: 61  LHNLNFS-DGHRQVTGKKSNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRV 120

Query: 121 ASLIRRLSNGGAATTPAEQGNSFKVANFATDVISG 153
           A+L+RRLS+GGA        +S+K ANFATDVISG
Sbjct: 121 AALVRRLSHGGA-------DSSYKAANFATDVISG 130

BLAST of Sgr028836 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.8e-27
Identity = 81/144 (56.25%), Postives = 98/144 (68.06%), Query Frame = 0

Query: 9   LLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSD 68
           L S L  L   +  AAAAA  SSS+         FQYLNVK  K D +D  + H L+FS 
Sbjct: 6   LFSSLLFLLVVIVGAAAAARLSSST--------KFQYLNVKATKLDFNDGQILHALNFS- 65

Query: 69  DGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGG 128
           DGHR V G K++++ FKL L+HRDK  LSHVHGHR GF+DRMKRDA RVA+L+RRLS+G 
Sbjct: 66  DGHRQVSGYKSDNNTFKLNLLHRDK--LSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA 125

Query: 129 AATTPAEQGNSFKVANFATDVISG 153
            A   A + + +KVANFATDVISG
Sbjct: 126 PA---AVKDSRYKVANFATDVISG 135

BLAST of Sgr028836 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 9.9e-27
Identity = 79/144 (54.86%), Postives = 95/144 (65.97%), Query Frame = 0

Query: 9   LLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSD 68
           L+S L      +   AAAA PSS +         FQYLNVK  K D +D  + H L+FS 
Sbjct: 6   LISSLLFFLAVLVVVAAAARPSSPT--------KFQYLNVKATKLDFNDGQILHTLNFS- 65

Query: 69  DGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGG 128
           D HR V G K+++  FKL L+HRDK  LSHVHGHR GF+DRMKRDA RVA+L+RRLS+G 
Sbjct: 66  DAHRQVSGHKSHNDTFKLNLLHRDK--LSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA 125

Query: 129 AATTPAEQGNSFKVANFATDVISG 153
           AA     + + FKVANFATDVISG
Sbjct: 126 AAV----EDSRFKVANFATDVISG 134

BLAST of Sgr028836 vs. ExPASy TrEMBL
Match: A0A6J1HQG0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466434 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 76/138 (55.07%), Postives = 92/138 (66.67%), Query Frame = 0

Query: 15  LLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDFSDDGHRLV 74
           LLFF+V+           S SSS     FQYLNVK  KFD +D  + H L+FS +GHRLV
Sbjct: 10  LLFFSVSVVL-------GSRSSS---VEFQYLNVKSNKFDVNDSQLLHTLNFS-NGHRLV 69

Query: 75  MGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGGAATTPA 134
            G K+N +KFKLKLVHRDKLP  H+HG   GF +R+KRD +RVA+L+RRLS     T+  
Sbjct: 70  TGRKSNHTKFKLKLVHRDKLP--HIHGQHQGFDERIKRDVKRVATLVRRLSRDDTTTSVV 129

Query: 135 EQGNSFKVANFATDVISG 153
           E  NS+KVANF T VISG
Sbjct: 130 ED-NSYKVANFGTGVISG 133

BLAST of Sgr028836 vs. ExPASy TrEMBL
Match: A0A6J1KA06 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493608 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.0e-23
Identity = 76/146 (52.05%), Postives = 92/146 (63.01%), Query Frame = 0

Query: 7   SPLLSLLHLLFFTVAAAAAAANPSSSSTSSSRHHANFQYLNVKEIKFDPDDDLVFHNLDF 66
           SPLL LLHLLF   AAAAA A PS           NF YLNVK  K + +   +   L+F
Sbjct: 8   SPLLLLLHLLFL-AAAAAALARPSLP--------PNFLYLNVKPTKLELNHTQILPTLNF 67

Query: 67  SDDGHRLVMGGKTNSSKFKLKLVHRDKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSN 126
           S D H  V   K +++  KL L+HRDKLP  H H HRH F++R+KRDA RVA+L+RRLS+
Sbjct: 68  S-DAHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHRHAFNERIKRDAIRVATLVRRLSH 127

Query: 127 GGAATTPAEQGNSFKVANFATDVISG 153
               T  A Q NS+KVANFAT V+SG
Sbjct: 128 ----TAAAVQDNSYKVANFATGVVSG 139

BLAST of Sgr028836 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-09
Identity = 40/121 (33.06%), Postives = 66/121 (54.55%), Query Frame = 0

Query: 33  STSSSRHHANFQYLNVKEIKFDPDDDLV-FHNLDFSDDGHRLVMGGKTNSSKFKLKLVHR 92
           S+SSS    +FQ ++V +        L  F+N  FSD+          +SSK+ L+L+HR
Sbjct: 17  SSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE----------SSSKYTLRLLHR 76

Query: 93  DKLPLSHVHGHRHGFHDRMKRDAQRVASLIRRLSNGGAATTPAEQGNSFKVANFATDVIS 152
           D+ P      H H  H RM+RD  RV++++RR+S       P+   + ++V +F +D++S
Sbjct: 77  DRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---GKVIPSSD-SRYEVNDFGSDIVS 123