Sgr028835 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr028835
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	NEDD8-specific protease 1-like
Location	tig00153207: 1175554 .. 1176207 (-)
RNA-Seq Expression	Sgr028835
Synteny	Sgr028835

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTTCTCGGAGATCCGATTGCAGATTCTGGAAGGGCGAAACTTTCTGAACGACACCATCATGGATTTCTACTTCTCCTATCTCTCTTCTTGGTACCAATCGGACGACATCCTCCTGGTTCCGGCGAGCATCTCGTTTTTTCTGGCCAACGCACCCGACTGGGAGAGTTTCTTCGCCACGATGGAATCTCTGAACCTCCATGAAAAGCAACTCGTCTTCTTCACTGTTAACGACAGCTCCAATTTCTCATCCAGCTCCAGCGGCGGATCTCACTGGAGCTTGCTCGTCTACTGCAGAACACGCAACTTGTTCATGCATTACGACAGCTTGAAGTGGAAGAACTACGAATGTGCTTTCTCCATCTATGAATCCATGAAGAACTACATCAGTTACGATACGGCGGAGTTCGTCGAGTGTCGCGCCCCTCGACAGACCAATGGATACGATTGCGGAGTGTACGTGATGGAAATTGCTAGGGCTATCTGCGATTGCTATTTCGATGGCGATCGATTCGATAGCGATGGTGAGATCAGTAGGTCGTTTGATGTGATGGAAAGTGTGAATGTTCGACATGTTGAGTCGAATATGCGTTCCGAATTGTTGAAATTGATATTCACATTGAAGGAAGAAGCTCGATACAACTTTCAATAG

mRNA sequence

ATGTCGTTCTCGGAGATCCGATTGCAGATTCTGGAAGGGCGAAACTTTCTGAACGACACCATCATGGATTTCTACTTCTCCTATCTCTCTTCTTGGTACCAATCGGACGACATCCTCCTGGTTCCGGCGAGCATCTCGTTTTTTCTGGCCAACGCACCCGACTGGGAGAGTTTCTTCGCCACGATGGAATCTCTGAACCTCCATGAAAAGCAACTCGTCTTCTTCACTGTTAACGACAGCTCCAATTTCTCATCCAGCTCCAGCGGCGGATCTCACTGGAGCTTGCTCGTCTACTGCAGAACACGCAACTTGTTCATGCATTACGACAGCTTGAAGTGGAAGAACTACGAATGTGCTTTCTCCATCTATGAATCCATGAAGAACTACATCAGTTACGATACGGCGGAGTTCGTCGAGTGTCGCGCCCCTCGACAGACCAATGGATACGATTGCGGAGTGTACGTGATGGAAATTGCTAGGGCTATCTGCGATTGCTATTTCGATGGCGATCGATTCGATAGCGATGGTGAGATCAGTAGGTCGTTTGATGTGATGGAAAGTGTGAATGTTCGACATGTTGAGTCGAATATGCGTTCCGAATTGTTGAAATTGATATTCACATTGAAGGAAGAAGCTCGATACAACTTTCAATAG

Coding sequence (CDS)

ATGTCGTTCTCGGAGATCCGATTGCAGATTCTGGAAGGGCGAAACTTTCTGAACGACACCATCATGGATTTCTACTTCTCCTATCTCTCTTCTTGGTACCAATCGGACGACATCCTCCTGGTTCCGGCGAGCATCTCGTTTTTTCTGGCCAACGCACCCGACTGGGAGAGTTTCTTCGCCACGATGGAATCTCTGAACCTCCATGAAAAGCAACTCGTCTTCTTCACTGTTAACGACAGCTCCAATTTCTCATCCAGCTCCAGCGGCGGATCTCACTGGAGCTTGCTCGTCTACTGCAGAACACGCAACTTGTTCATGCATTACGACAGCTTGAAGTGGAAGAACTACGAATGTGCTTTCTCCATCTATGAATCCATGAAGAACTACATCAGTTACGATACGGCGGAGTTCGTCGAGTGTCGCGCCCCTCGACAGACCAATGGATACGATTGCGGAGTGTACGTGATGGAAATTGCTAGGGCTATCTGCGATTGCTATTTCGATGGCGATCGATTCGATAGCGATGGTGAGATCAGTAGGTCGTTTGATGTGATGGAAAGTGTGAATGTTCGACATGTTGAGTCGAATATGCGTTCCGAATTGTTGAAATTGATATTCACATTGAAGGAAGAAGCTCGATACAACTTTCAATAG

Protein sequence

MSFSEIRLQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYISYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNVRHVESNMRSELLKLIFTLKEEARYNFQ

Homology

[Close]

BLAST of Sgr028835 vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 285.0 bits (728), Expect = 5.3e-73
Identity = 139/200 (69.50%), Postives = 166/200 (83.00%), Query Frame = 0


Query: 11  LEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEK 70
           LE  N+LND I+DFYF+YL   YQSDDILLVP SISF L NAPDW++F AT+ESLN HEK
Sbjct: 21  LEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLGNAPDWDTFIATVESLNFHEK 80

Query: 71  QLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYI 130
           +L+FFTVND+SNF  SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF+IYES+ +YI
Sbjct: 81  KLIFFTVNDNSNF--SSSGGSHWSLLVYCRKRNLFMHYDSLHMMNNESAFAIYESVNSYI 140

Query: 131 SYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNV 190
           SYD+AEF+ECRAPRQ NGYDCG+YVMEIAR I + YF+ D+F ++ EI  SF+V++ VN 
Sbjct: 141 SYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQFQNEDEICGSFNVVQRVNC 200

Query: 191 RHVESNMRSELLKLIFTLKE 211
           +HVESNMRSE+L+LI  L +
Sbjct: 201 KHVESNMRSEMLQLILGLNQ 218

[Close]

BLAST of Sgr028835 vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 272.3 bits (695), Expect = 3.6e-69
Identity = 133/196 (67.86%), Postives = 160/196 (81.63%), Query Frame = 0


Query: 11  LEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEK 70
           LE  N+LND I+DFYF+YL   YQSDDILL+  SISF L NA DW++F AT+ESLN HEK
Sbjct: 21  LEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLGNASDWDTFVATVESLNFHEK 80

Query: 71  QLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYI 130
           +L+FFTVND+SNF  SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF+IYES+ +YI
Sbjct: 81  KLIFFTVNDNSNF--SSSGGSHWSLLVYCRKRNLFMHYDSLHMMNNESAFAIYESVNSYI 140

Query: 131 SYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNV 190
           SYD+AEF+ECRAPRQ N YDCG+YVMEIAR I + YF+ D+F ++ EI  SF+V++SVN 
Sbjct: 141 SYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQFQNEDEICGSFNVVQSVNC 200

Query: 191 RHVESNMRSELLKLIF 207
           +HVESNMRSE + + F
Sbjct: 201 KHVESNMRSESINIYF 214

[Close]

BLAST of Sgr028835 vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 270.4 bits (690), Expect = 1.4e-68
Identity = 133/204 (65.20%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L  LEGRN+LNDTI+DFYF+YLS+ YQS++IL +P S+SF L N  DW+ F +T+ESLNL
Sbjct: 18  LHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLGNTLDWDIFISTIESLNL 77

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
           HEK+L+FF VND+SN   SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF++YES+ 
Sbjct: 78  HEKKLIFFVVNDNSNV--SSSGGSHWSLLVYCRKRNLFMHYDSLSTMNSEPAFTLYESVS 137

Query: 128 NYISYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMES 187
            YI+YD AEF+ECRAPRQ NGYDCG+YVME AR I + YF+ D+F S  EIS   +VM S
Sbjct: 138 GYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQFRSKDEISGPINVMRS 197

Query: 188 VNVRHVESNMRSELLKLIFTLKEE 212
           V  ++VESNMRSE+++LI  LK+E
Sbjct: 198 VKSKYVESNMRSEMMQLIRRLKQE 219

[Close]

BLAST of Sgr028835 vs. NCBI nr
Match: KAG7014407.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 269.6 bits (688), Expect = 2.3e-68
Identity = 131/204 (64.22%), Postives = 164/204 (80.39%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L  LEGRN+LNDTI+DFYF+YLS+ YQS++IL +P S+S+ L N  DW+ F +T+ESLNL
Sbjct: 18  LHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTIESLNL 77

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
           HEK+L+FF +ND+SN   SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF++YES+ 
Sbjct: 78  HEKKLIFFVINDNSNV--SSSGGSHWSLLVYCRKRNLFMHYDSLSTMNSEPAFTLYESVS 137

Query: 128 NYISYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMES 187
            YI+YD AEF+ECRAPRQ NGYDCG+YVME AR I + YF+ D+F S  EIS + +VM S
Sbjct: 138 GYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQFRSKDEISGTINVMRS 197

Query: 188 VNVRHVESNMRSELLKLIFTLKEE 212
           V  ++VESNMRSE+++LI  LK+E
Sbjct: 198 VKSKYVESNMRSEMMQLIRRLKQE 219

[Close]

BLAST of Sgr028835 vs. NCBI nr
Match: KAG7031003.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 264.6 bits (675), Expect = 7.4e-67
Identity = 129/188 (68.62%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 11  LEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEK 70
           LE  N+LND I+DFYF+YL   YQ DDILL+P SISF L NA DW++F AT+ESLN HEK
Sbjct: 21  LEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGNASDWDTFLATVESLNFHEK 80

Query: 71  QLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYI 130
           +L+FFTVND+SNF  SSSGGSHWSLLVYCR RNLFMHY SL   N E AF+IYES+ +YI
Sbjct: 81  KLIFFTVNDNSNF--SSSGGSHWSLLVYCRKRNLFMHYHSLHMMNNESAFAIYESVNSYI 140

Query: 131 SYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNV 190
           SYD+AEF+ECRAPRQ N YDCG+YVMEIAR I + YF+ D+F ++ EI  SF+V++SVN 
Sbjct: 141 SYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQFQNEDEICGSFNVVQSVNR 200

Query: 191 RHVESNMR 199
           +HVESNMR
Sbjct: 201 KHVESNMR 206

[Close]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.1e-31
Identity = 78/206 (37.86%), Postives = 119/206 (57.77%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L IL G  FLND +++FY S+LS+ + S  I L+P SI+F+++N PD E     M+ LNL
Sbjct: 23  LDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCPDTEYLKDFMKPLNL 82

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
            +K L+   VND+SN    + GG HWSLLVY +  N F+H+DS    N   A  +++++ 
Sbjct: 83  RDKDLLILPVNDNSNV-EVAEGGLHWSLLVYYKEANTFVHHDSYMGVNRWSAKQLFKAVS 142

Query: 128 NYISYDTAEFVECR-APRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVME 187
            ++S   A + EC   P+Q NGYDCGV+++  AR IC+ +  G   + D      +    
Sbjct: 143 PFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICEWFSSGGMKNRD----ELWFANV 202

Query: 188 SVNVRHVESNMRSELLKLIFTLKEEA 213
              V  + +++R E+L LI  L  E+
Sbjct: 203 KETVPDLVNHLREEILALIKKLMSES 223

[Close]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-20
Identity = 59/165 (35.76%), Postives = 89/165 (53.94%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWY---QSDDILLVPASISFFLANAPDWESFFATMES 67
           + +L+  N+LND I+ F F Y +S      SD +  +   ++ F+            +E 
Sbjct: 18  VSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQFIKCTSSPAEIAMFLEP 77

Query: 68  LNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYE 127
           L+L  K++VF  +ND+SN    ++GG+HWSLLVY + +N F HYDS    N   A  + E
Sbjct: 78  LDLPHKRVVFLAINDNSN---QAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSIHAKQVAE 137

Query: 128 SMKNYISY--DTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYF 168
            +K ++    D   FVE +AP Q N YDCG+YV+    A+C   F
Sbjct: 138 KLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQNLF 179

[Close]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 57/165 (34.55%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYL--SSWYQ-SDDILLVPASISFFLANAPDWESFFATMES 67
           + +L+  ++LND I+ F F Y   S ++  SD +  +   ++ F+    +       +E 
Sbjct: 18  VSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEP 77

Query: 68  LNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYE 127
           L+L  K++VF  +ND+SN    ++GG+HWSLLVY + +N F HYDS    N   A  + E
Sbjct: 78  LDLPNKRVVFLAINDNSN---QAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAE 137

Query: 128 SMKNYISY--DTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYF 168
            ++ ++    D   FVE +AP Q N YDCG+YV+    A+C  +F
Sbjct: 138 KLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFF 179

[Close]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 58/165 (35.15%), Postives = 90/165 (54.55%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYL--SSWYQ-SDDILLVPASISFFLANAPDWESFFATMES 67
           + +L+  ++LND I+ F F Y   S ++  SD +  +   ++ F+            +E 
Sbjct: 18  VSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQFIKCTSSPAEIAMFLEP 77

Query: 68  LNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYE 127
           L+L  K++VF  +ND+SN    ++GG+HWSLLVY + +N F HYDS    N   A  + E
Sbjct: 78  LDLPHKRVVFLAINDNSN---QAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSIHAKQVAE 137

Query: 128 SMKNYISY--DTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYF 168
            +K ++    D   FVE +AP Q N YDCG+YV+    A+C   F
Sbjct: 138 KLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQSLF 179

[Close]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-13
Identity = 50/166 (30.12%), Postives = 90/166 (54.22%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQS------DDILLVPASISF---FLANAPDWESF 67
           + IL+   +LND+I+ FY  +L    +       + +LL+  S+ F   F+ +  + +  
Sbjct: 19  INILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSVVFCCSFVESEQEIQLM 78

Query: 68  FATMESLNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYEC 127
           F   + L+L  K+++FF + ++ +  +   GG+HWSLL++ ++ N F++YDS+   N   
Sbjct: 79  FE--QPLSLKTKEVIFFPLTNNRD-PNVIGGGTHWSLLIFIKSLNKFIYYDSINSFNSSD 138

Query: 128 AFSIYESMKNYIS-----YDTAEF-VECRAPRQTNGYDCGVYVMEI 159
           A  I    K  +S      +  EF +  + P+Q NGYDCG+YV+ I
Sbjct: 139 AIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSI 181

[Close]

BLAST of Sgr028835 vs. ExPASy TrEMBL
Match: A0A6J1JKF6 (NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.6e-73
Identity = 139/200 (69.50%), Postives = 166/200 (83.00%), Query Frame = 0


Query: 11  LEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEK 70
           LE  N+LND I+DFYF+YL   YQSDDILLVP SISF L NAPDW++F AT+ESLN HEK
Sbjct: 21  LEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLGNAPDWDTFIATVESLNFHEK 80

Query: 71  QLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYI 130
           +L+FFTVND+SNF  SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF+IYES+ +YI
Sbjct: 81  KLIFFTVNDNSNF--SSSGGSHWSLLVYCRKRNLFMHYDSLHMMNNESAFAIYESVNSYI 140

Query: 131 SYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNV 190
           SYD+AEF+ECRAPRQ NGYDCG+YVMEIAR I + YF+ D+F ++ EI  SF+V++ VN 
Sbjct: 141 SYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQFQNEDEICGSFNVVQRVNC 200

Query: 191 RHVESNMRSELLKLIFTLKE 211
           +HVESNMRSE+L+LI  L +
Sbjct: 201 KHVESNMRSEMLQLILGLNQ 218

[Close]

BLAST of Sgr028835 vs. ExPASy TrEMBL
Match: A0A6J1FTN2 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.7e-69
Identity = 133/196 (67.86%), Postives = 160/196 (81.63%), Query Frame = 0


Query: 11  LEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNLHEK 70
           LE  N+LND I+DFYF+YL   YQSDDILL+  SISF L NA DW++F AT+ESLN HEK
Sbjct: 21  LEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLGNASDWDTFVATVESLNFHEK 80

Query: 71  QLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMKNYI 130
           +L+FFTVND+SNF  SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF+IYES+ +YI
Sbjct: 81  KLIFFTVNDNSNF--SSSGGSHWSLLVYCRKRNLFMHYDSLHMMNNESAFAIYESVNSYI 140

Query: 131 SYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMESVNV 190
           SYD+AEF+ECRAPRQ N YDCG+YVMEIAR I + YF+ D+F ++ EI  SF+V++SVN 
Sbjct: 141 SYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQFQNEDEICGSFNVVQSVNC 200

Query: 191 RHVESNMRSELLKLIF 207
           +HVESNMRSE + + F
Sbjct: 201 KHVESNMRSESINIYF 214

[Close]

BLAST of Sgr028835 vs. ExPASy TrEMBL
Match: A0A6J1GPM3 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 6.5e-69
Identity = 133/204 (65.20%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L  LEGRN+LNDTI+DFYF+YLS+ YQS++IL +P S+SF L N  DW+ F +T+ESLNL
Sbjct: 18  LHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLGNTLDWDIFISTIESLNL 77

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
           HEK+L+FF VND+SN   SSSGGSHWSLLVYCR RNLFMHYDSL   N E AF++YES+ 
Sbjct: 78  HEKKLIFFVVNDNSNV--SSSGGSHWSLLVYCRKRNLFMHYDSLSTMNSEPAFTLYESVS 137

Query: 128 NYISYDTAEFVECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVMES 187
            YI+YD AEF+ECRAPRQ NGYDCG+YVME AR I + YF+ D+F S  EIS   +VM S
Sbjct: 138 GYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQFRSKDEISGPINVMRS 197

Query: 188 VNVRHVESNMRSELLKLIFTLKEE 212
           V  ++VESNMRSE+++LI  LK+E
Sbjct: 198 VKSKYVESNMRSEMMQLIRRLKQE 219

[Close]

BLAST of Sgr028835 vs. ExPASy TrEMBL
Match: A0A7C8Z2M2 (ULP_PROTEASE domain-containing protein OS=Opuntia streptacantha OX=393608 PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 3.0e-37
Identity = 94/202 (46.53%), Postives = 130/202 (64.36%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L IL G  FLND I++FYFSYLSS Y+SDDILLVP SI+F+L N PD ESF A +E L+L
Sbjct: 23  LDILSGPYFLNDRIIEFYFSYLSSTYRSDDILLVPPSIAFWLQNYPDEESFKAFIEPLSL 82

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
             K +++F VN++ +F + + GGSHWSLLV+ R  N+F+H+DS +  N   A  +Y+S+ 
Sbjct: 83  STKNMIYFPVNNNEDF-TLAEGGSHWSLLVFYREANVFVHHDSFQGSNCSYAKRLYKSVA 142

Query: 128 NYISYDTAEF----VECRAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFD 187
            +++   +E      EC +P+Q NGYDCG++V+ IAR +C  Y  G   DSD      F 
Sbjct: 143 LFVASPDSETRYLEQEC-SPQQQNGYDCGLFVIAIARVLCSWYMGGGSVDSD---DLWFS 202

Query: 188 VMESVNVRHVESNMRSELLKLI 206
           V++          MRSE+L LI
Sbjct: 203 VVKEQVTPSAVLKMRSEILDLI 219

[Close]

BLAST of Sgr028835 vs. ExPASy TrEMBL
Match: A0A7J6VHN4 (Sentrin-specific protease OS=Thalictrum thalictroides OX=46969 GN=FRX31_025797 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 6.6e-37
Identity = 93/203 (45.81%), Postives = 127/203 (62.56%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L IL GR+FLND I++FYFSYL+S Y S+DILLVP SISF++ N PD ES    +E L  
Sbjct: 19  LDILSGRHFLNDRIIEFYFSYLASCYPSEDILLVPPSISFWIGNCPDLESLKDFVEPLKF 78

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
            +K++V FTVND+ +  S + GG HWSLL Y R +N+F+H+DSL   N   A  +Y+ + 
Sbjct: 79  PDKKMVIFTVNDNEDV-SMAEGGCHWSLLAYERKKNVFVHHDSLAGSNRWHAKRLYKVVV 138

Query: 128 NYISYDTAE----FVEC-RAPRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSF 187
            ++     E    F++C  +P+Q NGYDCG+YV  IA+ IC+ Y  G   D D      F
Sbjct: 139 RFMGISATEVDAPFIDCTTSPQQINGYDCGLYVTAIAKVICEWYVTGRSEDMD----LWF 198

Query: 188 DVMESVNVRHVESNMRSELLKLI 206
             +++       SNMRSE+L LI
Sbjct: 199 TSLKTKITTSTVSNMRSEILLLI 216

[Close]

BLAST of Sgr028835 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 2.2e-32
Identity = 78/206 (37.86%), Postives = 119/206 (57.77%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATMESLNL 67
           L IL G  FLND +++FY S+LS+ + S  I L+P SI+F+++N PD E     M+ LNL
Sbjct: 23  LDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCPDTEYLKDFMKPLNL 82

Query: 68  HEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNLFMHYDSLKWKNYECAFSIYESMK 127
            +K L+   VND+SN    + GG HWSLLVY +  N F+H+DS    N   A  +++++ 
Sbjct: 83  RDKDLLILPVNDNSNV-EVAEGGLHWSLLVYYKEANTFVHHDSYMGVNRWSAKQLFKAVS 142

Query: 128 NYISYDTAEFVECR-APRQTNGYDCGVYVMEIARAICDCYFDGDRFDSDGEISRSFDVME 187
            ++S   A + EC   P+Q NGYDCGV+++  AR IC+ +  G   + D      +    
Sbjct: 143 PFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICEWFSSGGMKNRD----ELWFANV 202

Query: 188 SVNVRHVESNMRSELLKLIFTLKEEA 213
              V  + +++R E+L LI  L  E+
Sbjct: 203 KETVPDLVNHLREEILALIKKLMSES 223

[Close]

BLAST of Sgr028835 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.1e-04
Identity = 41/192 (21.35%), Postives = 75/192 (39.06%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATM----- 67
           +++L+   F+NDTI+DFY +YL +  Q+++        SFF     D +   +++     
Sbjct: 433 VELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKA 492

Query: 68  ---------ESLNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNL--------- 127
                      +++  K  +F  VN +           HWSL+V C    +         
Sbjct: 493 AFLRVRKWTRKVDMFGKDYIFVPVNYN----------LHWSLIVICHPGEVANRTDLDLD 552

Query: 128 -------FMHYDSLK-----WKNYECAFSIYESMKNY--------ISYDTAEFVECRAPR 157
                   +H DS+K      KN    +   E  + +          +    FV    P+
Sbjct: 553 DSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQ 612

[Close]

BLAST of Sgr028835 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.1e-04
Identity = 41/192 (21.35%), Postives = 75/192 (39.06%), Query Frame = 0


Query: 8   LQILEGRNFLNDTIMDFYFSYLSSWYQSDDILLVPASISFFLANAPDWESFFATM----- 67
           +++L+   F+NDTI+DFY +YL +  Q+++        SFF     D +   +++     
Sbjct: 401 VELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKA 460

Query: 68  ---------ESLNLHEKQLVFFTVNDSSNFSSSSSGGSHWSLLVYCRTRNL--------- 127
                      +++  K  +F  VN +           HWSL+V C    +         
Sbjct: 461 AFLRVRKWTRKVDMFGKDYIFVPVNYN----------LHWSLIVICHPGEVANRTDLDLD 520

Query: 128 -------FMHYDSLK-----WKNYECAFSIYESMKNY--------ISYDTAEFVECRAPR 157
                   +H DS+K      KN    +   E  + +          +    FV    P+
Sbjct: 521 DSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQ 580

The following BLAST results are available for this feature:

BLAST of Sgr028835 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988745.1	5.3e-73	69.50	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]
XP_022942008.1	3.6e-69	67.86	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_022953908.1	1.4e-68	65.20	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
KAG7014407.1	2.3e-68	64.22	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG7031003.1	7.4e-67	68.62	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Sgr028835 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	3.1e-31	37.86	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q5FVJ8	3.2e-20	35.76	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q96LD8	1.2e-19	34.55	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q9D2Z4	1.2e-19	35.15	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q54XR2	1.7e-13	30.12	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Sgr028835 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKF6	2.6e-73	69.50	NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 ...	[more]
A0A6J1FTN2	1.7e-69	67.86	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=...	[more]
A0A6J1GPM3	6.5e-69	65.20	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=...	[more]
A0A7C8Z2M2	3.0e-37	46.53	ULP_PROTEASE domain-containing protein OS=Opuntia streptacantha OX=393608 PE=3 S...	[more]
A0A7J6VHN4	6.6e-37	45.81	Sentrin-specific protease OS=Thalictrum thalictroides OX=46969 GN=FRX31_025797 P...	[more]

BLAST of Sgr028835 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	2.2e-32	37.86	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	1.1e-04	21.35	Cysteine proteinases superfamily protein	[more]
AT1G09730.2	1.1e-04	21.35	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 2..211 e-value: 3.5E-50 score: 172.7
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 136..209
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 5..131
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 16..161 e-value: 3.4E-10 score: 40.1
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 1..162 score: 15.070087
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 136..209 coord: 5..131
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 8..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr028835.1	Sgr028835.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity