Sgr028600 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr028600
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionheat stress transcription factor B-4-like
Locationtig00153204: 2678768 .. 2679989 (-)
RNA-Seq ExpressionSgr028600
SyntenySgr028600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTGATGCTGGACAATTGCGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTCCCAGCTCCATTTCTCTCCAAAACCTATCAGCTTGTCGATGATCCCTCCACTGATCACATTGTCTCCTGGGGTGAAGATGACACCACCTTCGTCGTCTGGCGTCCTCCTGAGTTTGCTCGCGATCTCCTTCCCAACTACTTCAAGCATAATAACTTCTCCAGTTTCGTTCGCCAGCTCAACACTTATGTACGTATTCCCCCCCCCCCCTCTCTCTCTCTCTATAACAACATTCAGAAAATATTAGTTTTGGCTCTCTCTAATTTGTTTTGTTTTGTTTTGTTTTTGGTGTTGCAAAATATAGGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCGAACGAGTTCTTCAGAAAGGGAGAGAAGCATTTACTATGTGAGATCCACAGGAGAAAAACTGCTCAGCCGCAAGTGGCCTACAACCATCACCTCCAACAGCATTCTTCTCTGGGTGTTACCGGGCCGAGTTTCTTCCCGTTCTCAAGTCGAGTTAGCATCTCGCCGTCGGACTCAGACGAGCAAGGGAATTGGTGCGACTCGCCACCAGGACCACCGAGGGTTGCCGGAGGATTCTCAGTTCTGGCCGCTGCCAATAGTAATAATAATACAAATTACAACATGATCAACTCAGTTACCGCTTTATCTGAAGATAACGAGAGGTTGAGGAGGAACAACTCTATGCTGATTTCCGAGCTTGCCCACATGAGGAAACTTTACAACGACATTATCTACTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCAAGCAACTCTTTCCCTTCTTTGCTTCTTTGTCATCCACCTTCTCAGAAACCCCTCAACCAGTTTCTTGGATTCTATCCTCCTAGTCCTAACCCTACTAAGCAGCAGCCTGCTCCTGCTGCAGCTATTGCTACGGCTCACTTTGTTAGCTCTCCGACAGCCACGTCGAAGAGCGCAGTTACGGAGCTCGACACCTTCGTGGGTGAGCGTGGGATTGAGAGCAACAACAGCTGCAAAACCAAGCTGTTTGGAGTGCCACTTCCATCCAAGAAAAGACTGCACCCTGATAGTTACGTTTCTATTTCCTCCAACACAGATACCCACAAGAGCTCTCGCCTCGTCTTAGACAAAGGCGACAATTTGGGGCTCAATCTTATGCCTCCTTCTTCATGCTAG

mRNA sequence

ATGTCTCTGATGCTGGACAATTGCGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTCCCAGCTCCATTTCTCTCCAAAACCTATCAGCTTGTCGATGATCCCTCCACTGATCACATTGTCTCCTGGGGTGAAGATGACACCACCTTCGTCGTCTGGCGTCCTCCTGAGTTTGCTCGCGATCTCCTTCCCAACTACTTCAAGCATAATAACTTCTCCAGTTTCGTTCGCCAGCTCAACACTTATGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCGAACGAGTTCTTCAGAAAGGGAGAGAAGCATTTACTATGTGAGATCCACAGGAGAAAAACTGCTCAGCCGCAAGTGGCCTACAACCATCACCTCCAACAGCATTCTTCTCTGGGTGTTACCGGGCCGAGTTTCTTCCCGTTCTCAAGTCGAGTTAGCATCTCGCCGTCGGACTCAGACGAGCAAGGGAATTGGTGCGACTCGCCACCAGGACCACCGAGGGTTGCCGGAGGATTCTCAGTTCTGGCCGCTGCCAATAGTAATAATAATACAAATTACAACATGATCAACTCAGTTACCGCTTTATCTGAAGATAACGAGAGGTTGAGGAGGAACAACTCTATGCTGATTTCCGAGCTTGCCCACATGAGGAAACTTTACAACGACATTATCTACTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCAAGCAACTCTTTCCCTTCTTTGCTTCTTTGTCATCCACCTTCTCAGAAACCCCTCAACCAGTTTCTTGGATTCTATCCTCCTAGTCCTAACCCTACTAAGCAGCAGCCTGCTCCTGCTGCAGCTATTGCTACGGCTCACTTTGTTAGCTCTCCGACAGCCACGTCGAAGAGCGCAGTTACGGAGCTCGACACCTTCGTGGGTGAGCGTGGGATTGAGAGCAACAACAGCTGCAAAACCAAGCTGTTTGGAGTGCCACTTCCATCCAAGAAAAGACTGCACCCTGATAGTTACGTTTCTATTTCCTCCAACACAGATACCCACAAGAGCTCTCGCCTCGTCTTAGACAAAGGCGACAATTTGGGGCTCAATCTTATGCCTCCTTCTTCATGCTAG

Coding sequence (CDS)

ATGTCTCTGATGCTGGACAATTGCGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTCCCAGCTCCATTTCTCTCCAAAACCTATCAGCTTGTCGATGATCCCTCCACTGATCACATTGTCTCCTGGGGTGAAGATGACACCACCTTCGTCGTCTGGCGTCCTCCTGAGTTTGCTCGCGATCTCCTTCCCAACTACTTCAAGCATAATAACTTCTCCAGTTTCGTTCGCCAGCTCAACACTTATGGTTTTAGGAAGATTGTACCGGACAGATGGGAGTTTGCGAACGAGTTCTTCAGAAAGGGAGAGAAGCATTTACTATGTGAGATCCACAGGAGAAAAACTGCTCAGCCGCAAGTGGCCTACAACCATCACCTCCAACAGCATTCTTCTCTGGGTGTTACCGGGCCGAGTTTCTTCCCGTTCTCAAGTCGAGTTAGCATCTCGCCGTCGGACTCAGACGAGCAAGGGAATTGGTGCGACTCGCCACCAGGACCACCGAGGGTTGCCGGAGGATTCTCAGTTCTGGCCGCTGCCAATAGTAATAATAATACAAATTACAACATGATCAACTCAGTTACCGCTTTATCTGAAGATAACGAGAGGTTGAGGAGGAACAACTCTATGCTGATTTCCGAGCTTGCCCACATGAGGAAACTTTACAACGACATTATCTACTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCAAGCAACTCTTTCCCTTCTTTGCTTCTTTGTCATCCACCTTCTCAGAAACCCCTCAACCAGTTTCTTGGATTCTATCCTCCTAGTCCTAACCCTACTAAGCAGCAGCCTGCTCCTGCTGCAGCTATTGCTACGGCTCACTTTGTTAGCTCTCCGACAGCCACGTCGAAGAGCGCAGTTACGGAGCTCGACACCTTCGTGGGTGAGCGTGGGATTGAGAGCAACAACAGCTGCAAAACCAAGCTGTTTGGAGTGCCACTTCCATCCAAGAAAAGACTGCACCCTGATAGTTACGTTTCTATTTCCTCCAACACAGATACCCACAAGAGCTCTCGCCTCGTCTTAGACAAAGGCGACAATTTGGGGCTCAATCTTATGCCTCCTTCTTCATGCTAG

Protein sequence

MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVAGGFSVLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSNSFPSLLLCHPPSQKPLNQFLGFYPPSPNPTKQQPAPAAAIATAHFVSSPTATSKSAVTELDTFVGERGIESNNSCKTKLFGVPLPSKKRLHPDSYVSISSNTDTHKSSRLVLDKGDNLGLNLMPPSSC
Homology
BLAST of Sgr028600 vs. NCBI nr
Match: KAG7029334.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 575.1 bits (1481), Expect = 4.4e-160
Identity = 314/389 (80.72%), Postives = 333/389 (85.60%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 88  MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 147

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 148 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 207

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLA 180
           + YNHH      LG ++G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL 
Sbjct: 208 LPYNHH-----HLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLG 267

Query: 181 AAN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240
           +AN  +N+NT+Y+M++SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV
Sbjct: 268 SANVSNNSNTSYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 327

Query: 241 SPSNSFPSLLLCHPP---------SQKPLNQFLGFYPPSPN---PTKQQPAPAA-AIATA 300
           SPSNSFPSLLL HP          SQKPLNQ LGFYPP P+    TK    P A AI T 
Sbjct: 328 SPSNSFPSLLLSHPSTTTTTPPPNSQKPLNQLLGFYPPPPHANTTTKHFSFPTANAIPTT 387

Query: 301 HFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYVSIS 360
           HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YVS S
Sbjct: 388 HFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTS 447

Query: 361 SN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           SN +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 448 SNVSDTNKNSRLVLEKGDNLGLNLMPPSS 467

BLAST of Sgr028600 vs. NCBI nr
Match: XP_022962526.1 (heat stress transcription factor B-4-like [Cucurbita moschata])

HSP 1 Score: 575.1 bits (1481), Expect = 4.4e-160
Identity = 314/389 (80.72%), Postives = 333/389 (85.60%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLA 180
           + YNHH      LG ++G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL 
Sbjct: 121 LPYNHH-----HLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLG 180

Query: 181 AAN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240
           +AN  +N+NT+Y+M++SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV
Sbjct: 181 SANVSNNSNTSYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240

Query: 241 SPSNSFPSLLLCHPP---------SQKPLNQFLGFYPPSPN---PTKQQPAPAA-AIATA 300
           SPSNSFPSLLL HP          SQKPLNQ LGFYPP P+    TK    P A AI T 
Sbjct: 241 SPSNSFPSLLLSHPSSTTTPPPPNSQKPLNQLLGFYPPPPHANTTTKHFSFPTANAIPTT 300

Query: 301 HFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYVSIS 360
           HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YVS S
Sbjct: 301 HFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTS 360

Query: 361 SN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           SN +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 SNVSDTNKNSRLVLEKGDNLGLNLMPPSS 380

BLAST of Sgr028600 vs. NCBI nr
Match: KAG6598364.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 574.7 bits (1480), Expect = 5.7e-160
Identity = 314/390 (80.51%), Postives = 333/390 (85.38%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLA 180
           + YNHH      LG ++G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL 
Sbjct: 121 LPYNHH-----HLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLG 180

Query: 181 AAN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240
           +AN  +N+NT+Y+M++SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV
Sbjct: 181 SANVSNNSNTSYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240

Query: 241 SPSNSFPSLLLCHPP----------SQKPLNQFLGFYPPSPN---PTKQQPAPAA-AIAT 300
           SPSNSFPSLLL HP           SQKPLNQ LGFYPP P+    TK    P A AI T
Sbjct: 241 SPSNSFPSLLLSHPSTTTTTPPPPNSQKPLNQLLGFYPPPPHANTTTKHFSFPTANAIPT 300

Query: 301 AHFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYVSI 360
            HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YVS 
Sbjct: 301 THFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVST 360

Query: 361 SSN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           SSN +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 SSNVSDTNKNSRLVLEKGDNLGLNLMPPSS 381

BLAST of Sgr028600 vs. NCBI nr
Match: XP_023545868.1 (heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.8 bits (1462), Expect = 6.9e-158
Identity = 308/384 (80.21%), Postives = 328/384 (85.42%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLA 180
           + YNHH      LG ++G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL 
Sbjct: 121 LPYNHH-----HLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLG 180

Query: 181 AAN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240
           +AN  +N+NT+Y+M++SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV
Sbjct: 181 SANVSNNSNTSYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240

Query: 241 SPSNSFPSLLLCHPP--------SQKPLNQFLGFYPP--SPNPTKQQPAPAA-AIATAHF 300
           SPSNSFPSLLL HP         SQKPLNQ LGFYPP  +   TK    P A AI T HF
Sbjct: 241 SPSNSFPSLLLSHPSTTTTPPPNSQKPLNQLLGFYPPTHANTTTKHFSFPTANAIPTTHF 300

Query: 301 VSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPDSYVSISSNTD 360
           VSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD+  +  S +D
Sbjct: 301 VSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDT-TNYVSTSD 360

Query: 361 THKSSRLVLDKGDNLGLNLMPPSS 368
           T+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 TNKNSRLVLEKGDNLGLNLMPPSS 374

BLAST of Sgr028600 vs. NCBI nr
Match: XP_022997563.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 565.8 bits (1457), Expect = 2.6e-157
Identity = 310/392 (79.08%), Postives = 327/392 (83.42%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLAA 180
           + YNHH          G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL +
Sbjct: 121 LPYNHH--------HLGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLGS 180

Query: 181 AN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS 240
           AN  +N+NT+Y+M+ SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS
Sbjct: 181 ANVSNNSNTSYSMMGSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS 240

Query: 241 PSNSFPSLLLCHPP--------------SQKPLNQFLGFYPP--SPNPTKQQPAPAA-AI 300
           PSNSFPSLLL HP               SQKPLNQ LGFYPP  +   TK    P A AI
Sbjct: 241 PSNSFPSLLLSHPSTTTTTPPPPPPPPNSQKPLNQLLGFYPPTHANTTTKHFSFPTANAI 300

Query: 301 ATAHFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYV 360
            T HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YV
Sbjct: 301 PTTHFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYV 360

Query: 361 SISSN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           S SS+ +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 STSSHVSDTNKNSRLVLEKGDNLGLNLMPPSS 380

BLAST of Sgr028600 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.3e-86
Identity = 204/384 (53.12%), Postives = 242/384 (63.02%), Query Frame = 0

Query: 1   MSLMLDNCEG---------ILLSLDSH-KSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTT 60
           M++M++N  G         I L ++   K+VPAPFL+KTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDEQGNWC-DSPP 180
           IHRRKT+Q  PQ    +  H      +  +G SFFP       +P   +E   WC DSPP
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSPP 180

Query: 181 GPPRVAGGFSVLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDI 240
             PRV       AA              VTALSEDNERLRR+N++L+SELAHM+KLYNDI
Sbjct: 181 SRPRVIPQQIDTAA-------------QVTALSEDNERLRRSNTVLMSELAHMKKLYNDI 240

Query: 241 IYFVQNHVKPVSPSNSFPSLLLCHPPSQKPLNQFLGFYPPSPNPTKQQPAPAAAIAT-AH 300
           IYFVQNHVKPV+PSN           +   L+ FL        PT      A   AT  +
Sbjct: 241 IYFVQNHVKPVAPSN-----------NSSYLSSFLQKQQQQQPPTLDYYNTATVNATNLN 300

Query: 301 FVSSPTATSKSAVTELDTFVGERGIESNNSCKTKLFGVPLP-SKKRLHPDSYVSISSNTD 360
            ++S   TS+S++T L+        +SN   KTKLFGV LP SKKR H  S        D
Sbjct: 301 ALNSSPPTSQSSITVLEDDHTNHHDQSNMR-KTKLFGVSLPSSKKRSHHFS--------D 347

Query: 361 THKSSRLVLDKGDNLGLNLMPPSS 368
               +RLVLD+ D L LNLM  S+
Sbjct: 361 QTSKTRLVLDQSD-LALNLMTAST 347

BLAST of Sgr028600 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 8.2e-69
Identity = 147/254 (57.87%), Postives = 173/254 (68.11%), Query Frame = 0

Query: 1   MSLMLDNCEG-ILLSLD-SH----------KSVPAPFLSKTYQLVDDPSTDHIVSWGEDD 60
           M+ +++ C G +++S++ SH           + PAPFLSKTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKT---AQPQVAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDE-QGNWCDSP 180
            EIHRRK+   +QPQ      + QH  L +  P   P S  V  + + +   Q  +C SP
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHFQEEYCSSP 180

Query: 181 PGPPRVAGGFSVLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYND 239
                 AGG   L A                ALSEDN +LRR NS+L+SELAHMRKLYND
Sbjct: 181 AD---YAGGGGDLLA----------------ALSEDNRQLRRRNSLLLSELAHMRKLYND 235

BLAST of Sgr028600 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 9.0e-68
Identity = 141/269 (52.42%), Postives = 171/269 (63.57%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLD----------SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTT 60
           M+ +++ C  +++S++          + K VPAPFL+KTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQP-------QVAYNHHLQQHSSLGVTGPSFF--------PFSSRVSISPSDSD 180
           IHRRK++QP       Q  ++HH     SL    P++         P ++       D  
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATTAHCTVAGDGG 180

Query: 181 EQGNWCDSPPGPPRVAGGFSVLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISEL 240
           E G++                                 + ALSEDN +LRR NS+L+SEL
Sbjct: 181 EGGDF---------------------------------LAALSEDNRQLRRRNSLLLSEL 236

Query: 241 AHMRKLYNDIIYFVQNHVKPVSPSNSFPS 245
           AHM+KLYNDIIYF+QNHV PV+ + + PS
Sbjct: 241 AHMKKLYNDIIYFLQNHVAPVTTTTTTPS 236

BLAST of Sgr028600 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-64
Identity = 171/393 (43.51%), Postives = 213/393 (54.20%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLPNYFKHNNFSS 76
           + K+VPAPFL+KTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LPNYFKHNNFSS
Sbjct: 17  AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSS 76

Query: 77  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAYNHHLQQHSSLGV 136
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+    A        S    
Sbjct: 77  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTA------SPSPPPF 136

Query: 137 TGPSFFPFSSRVSISPSDSDE-------------------QGNWCD-SPPGPPRVAGGFS 196
             P  FP      ++ +                       Q +W + + P   R+     
Sbjct: 137 FAPPHFPLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLALGG 196

Query: 197 VLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKP 256
              + +S          +   L E+NERLRR+N+ L+ ELAHMRKLYNDIIYFVQNHV+P
Sbjct: 197 PAPSPSSAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQNHVRP 256

Query: 257 VSPSNSFPSLLLCHPPSQKPLNQFLGFYPPSPNPTKQQPAPAAAIATAHFVSSPTATSKS 316
           V+PS           P+     Q LG         + +  PAAA    +   S T++S  
Sbjct: 257 VAPS-----------PAAAAFLQGLGM--------QARKKPAAANVLNNSGGSTTSSSSL 316

Query: 317 AVTELDT-------FVGER-GIESNN------SCKTKLFGVPLP-------SKKRLHPDS 368
            + E  +         GE+ G E+ N      S  TKLFGV L        SK+   P+ 
Sbjct: 317 TIAEEPSPPPQQQHLAGEKSGGEAGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPEE 376

BLAST of Sgr028600 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.9e-58
Identity = 152/337 (45.10%), Postives = 192/337 (56.97%), Query Frame = 0

Query: 21  VPAPFLSKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHNNFSS 80
           VPAPFL+KTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+NFSS
Sbjct: 22  VPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHSNFSS 81

Query: 81  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ------PQVAYNHHLQQ 140
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A       P         +
Sbjct: 82  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPFFAPR 141

Query: 141 HSSLGVTGPSFFPFSSRVSI----SPSDSDEQGNWCDSPPGPPRVAGGF-----SVLAAA 200
           H + G    +FF     +      +   + E+ +W +S   P   +         V+A A
Sbjct: 142 HFAAG----AFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPA 201

Query: 201 NSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSN 260
                T         AL ++N RL R N+ L+ ELAHMRKLY+DIIYFVQNHV+PV+PS 
Sbjct: 202 RRAAAT-----PEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPS- 261

Query: 261 SFPSLLLCHPPSQKPLNQFLGFYPPSPNPTKQQPAPAAAIATAHFVSSPTATSKSAVTEL 320
                     P+       LG   P P   K   APA+ +  A   S+ +++S +   + 
Sbjct: 262 ----------PAAAAALHGLGVLRPPPAGGK---APASEVRGASGRSATSSSSLTVAEDQ 321

Query: 321 DTFVG-------ERGIE----SNNSCKTKLFGVPLPS 327
            T +        E+ I      N    TKLFGV L S
Sbjct: 322 PTLLALRLPRTTEKIINEVSGGNGGGSTKLFGVHLSS 335

BLAST of Sgr028600 vs. ExPASy TrEMBL
Match: A0A6J1HDI0 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111462929 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.1e-160
Identity = 314/389 (80.72%), Postives = 333/389 (85.60%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLA 180
           + YNHH      LG ++G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL 
Sbjct: 121 LPYNHH-----HLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLG 180

Query: 181 AAN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240
           +AN  +N+NT+Y+M++SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV
Sbjct: 181 SANVSNNSNTSYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPV 240

Query: 241 SPSNSFPSLLLCHPP---------SQKPLNQFLGFYPPSPN---PTKQQPAPAA-AIATA 300
           SPSNSFPSLLL HP          SQKPLNQ LGFYPP P+    TK    P A AI T 
Sbjct: 241 SPSNSFPSLLLSHPSSTTTPPPPNSQKPLNQLLGFYPPPPHANTTTKHFSFPTANAIPTT 300

Query: 301 HFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYVSIS 360
           HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YVS S
Sbjct: 301 HFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTS 360

Query: 361 SN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           SN +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 SNVSDTNKNSRLVLEKGDNLGLNLMPPSS 380

BLAST of Sgr028600 vs. ExPASy TrEMBL
Match: A0A6J1KEA4 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111492453 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.3e-157
Identity = 310/392 (79.08%), Postives = 327/392 (83.42%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVA-GGFSVLAA 180
           + YNHH          G SFFPFS+RVSISPSDSDE  NWCDSPP PPR A  GFSVL +
Sbjct: 121 LPYNHH--------HLGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLGS 180

Query: 181 AN--SNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS 240
           AN  +N+NT+Y+M+ SVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS
Sbjct: 181 ANVSNNSNTSYSMMGSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVS 240

Query: 241 PSNSFPSLLLCHPP--------------SQKPLNQFLGFYPP--SPNPTKQQPAPAA-AI 300
           PSNSFPSLLL HP               SQKPLNQ LGFYPP  +   TK    P A AI
Sbjct: 241 PSNSFPSLLLSHPSTTTTTPPPPPPPPNSQKPLNQLLGFYPPTHANTTTKHFSFPTANAI 300

Query: 301 ATAHFVSSPTATSKSAVTELDTFVGERGIE-SNNSCKTKLFGVPL-PSKKRLHPD--SYV 360
            T HFVSSPTATSKSAVTELDTF    G+E  N+SCKTKLFGVPL  SKKR HPD  +YV
Sbjct: 301 PTTHFVSSPTATSKSAVTELDTF----GMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYV 360

Query: 361 SISSN-TDTHKSSRLVLDKGDNLGLNLMPPSS 368
           S SS+ +DT+K+SRLVL+KGDNLGLNLMPPSS
Sbjct: 361 STSSHVSDTNKNSRLVLEKGDNLGLNLMPPSS 380

BLAST of Sgr028600 vs. ExPASy TrEMBL
Match: A0A0A0LKU2 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.9e-155
Identity = 298/383 (77.81%), Postives = 325/383 (84.86%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRV--AGGFSVL 180
           + Y HH      LG + GPSFFPFS+RVSISPSDSDEQ NWCDSPP PPR    GG+SV+
Sbjct: 121 LPYTHH-----HLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVPPRATGTGGYSVM 180

Query: 181 AA---ANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVK 240
            +   +N+++ +NYNM++SVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVK
Sbjct: 181 GSGIISNNSSTSNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVK 240

Query: 241 PVSPSNSFPSLLLCHPP----SQKPLNQFLGFYPP-SPNPTKQQPAPAAAIATAHFVSSP 300
           PVSPSNSFPSLLL H P    SQKPLN FLG  PP + + T    +    I T HFVSSP
Sbjct: 241 PVSPSNSFPSLLLSHHPSTTNSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDTFVGERGIESNNSCKTKLFGVPL-PSKKRLHPD---SYVSISSN-TDT 360
           TATSKSAVTELD  VGE     NNSCKTKLFGV +  SKKR+HPD   +YVSI SN +DT
Sbjct: 301 TATSKSAVTELD--VGE---ADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDT 360

Query: 361 HKSSRLVLDKGDNLGLNLMPPSS 368
           +K+SRL+L+KGDNLGLNLMPPS+
Sbjct: 361 NKNSRLILEKGDNLGLNLMPPST 373

BLAST of Sgr028600 vs. ExPASy TrEMBL
Match: A0A1S3BAP2 (heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.6e-154
Identity = 298/384 (77.60%), Postives = 323/384 (84.11%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRV--AGGFSVL 180
           + Y+HH      LG + GPSFFPFS+RVSISPSDSDEQ NWCDSPP  PR    GG+SV+
Sbjct: 121 LPYSHH-----HLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVAPRATGTGGYSVM 180

Query: 181 AAANSNNN---TNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVK 240
            +   +NN   +NYNM++SVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVK
Sbjct: 181 GSGIISNNSRASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVK 240

Query: 241 PVSPSNSFPSLLLCHPP-----SQKPLNQFLGFYPP-SPNPTKQQPAPAAAIATAHFVSS 300
           PVSPSNSFPSLLL H P     SQKPLN FLG  PP + + T    +    I T HFVSS
Sbjct: 241 PVSPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSS 300

Query: 301 PTATSKSAVTELDTFVGERGIESNNSCKTKLFGVPL-PSKKRLHPD---SYVSISSN-TD 360
           PTATSKSAVTELD  VGE     NNSCKTKLFGV +  SKKR+HPD   +YVSI SN +D
Sbjct: 301 PTATSKSAVTELD--VGE---ADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSD 360

Query: 361 THKSSRLVLDKGDNLGLNLMPPSS 368
           T+K+SRL+L+KGDNLGLNLMPPS+
Sbjct: 361 TNKNSRLILEKGDNLGLNLMPPST 374

BLAST of Sgr028600 vs. ExPASy TrEMBL
Match: A0A5A7V551 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00440 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.1e-153
Identity = 297/384 (77.34%), Postives = 325/384 (84.64%), Query Frame = 0

Query: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VAYNHHLQQHSSLG-VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRV--AGGFSVL 180
           + Y+HH      LG + GPSFFPFS+RVSISPSDSDEQ NWCDSPP  PR    GG+SV+
Sbjct: 121 LPYSHH-----HLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPLAPRATGTGGYSVM 180

Query: 181 AA---ANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVK 240
            +   +N+++ +NYNM++SVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVK
Sbjct: 181 GSGIISNNSSASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVK 240

Query: 241 PVSPSNSFPSLLLCHPP-----SQKPLNQFLGFYPP-SPNPTKQQPAPAAAIATAHFVSS 300
           PVSPSNSFPSLLL H P     SQKPLN FLG  PP + + T    +    I T HFVSS
Sbjct: 241 PVSPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSS 300

Query: 301 PTATSKSAVTELDTFVGERGIESNNSCKTKLFGVPL-PSKKRLHPD---SYVSISSN-TD 360
           PTATSKSAVTELD  VGE     NNSCKTKLFGV +  SKKR+HPD   +YVSI SN +D
Sbjct: 301 PTATSKSAVTELD--VGE---ADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSD 360

Query: 361 THKSSRLVLDKGDNLGLNLMPPSS 368
           T+K+SRL+L+KGDNLGLNLMPPS+
Sbjct: 361 TNKNSRLILEKGDNLGLNLMPPST 374

BLAST of Sgr028600 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 320.1 bits (819), Expect = 2.3e-87
Identity = 204/384 (53.12%), Postives = 242/384 (63.02%), Query Frame = 0

Query: 1   MSLMLDNCEG---------ILLSLDSH-KSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTT 60
           M++M++N  G         I L ++   K+VPAPFL+KTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VAYNHHLQQHSSLGVTGPSFFPFSSRVSISPSDSDEQGNWC-DSPP 180
           IHRRKT+Q  PQ    +  H      +  +G SFFP       +P   +E   WC DSPP
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSPP 180

Query: 181 GPPRVAGGFSVLAAANSNNNTNYNMINSVTALSEDNERLRRNNSMLISELAHMRKLYNDI 240
             PRV       AA              VTALSEDNERLRR+N++L+SELAHM+KLYNDI
Sbjct: 181 SRPRVIPQQIDTAA-------------QVTALSEDNERLRRSNTVLMSELAHMKKLYNDI 240

Query: 241 IYFVQNHVKPVSPSNSFPSLLLCHPPSQKPLNQFLGFYPPSPNPTKQQPAPAAAIAT-AH 300
           IYFVQNHVKPV+PSN           +   L+ FL        PT      A   AT  +
Sbjct: 241 IYFVQNHVKPVAPSN-----------NSSYLSSFLQKQQQQQPPTLDYYNTATVNATNLN 300

Query: 301 FVSSPTATSKSAVTELDTFVGERGIESNNSCKTKLFGVPLP-SKKRLHPDSYVSISSNTD 360
            ++S   TS+S++T L+        +SN   KTKLFGV LP SKKR H  S        D
Sbjct: 301 ALNSSPPTSQSSITVLEDDHTNHHDQSNMR-KTKLFGVSLPSSKKRSHHFS--------D 347

Query: 361 THKSSRLVLDKGDNLGLNLMPPSS 368
               +RLVLD+ D L LNLM  S+
Sbjct: 361 QTSKTRLVLDQSD-LALNLMTAST 347

BLAST of Sgr028600 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.8e-47
Identity = 102/217 (47.00%), Postives = 135/217 (62.21%), Query Frame = 0

Query: 16  DSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS +S+P PFL+KTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAYNHHLQQHS-SLG 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +A        + +  
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 VTGPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVAGGFSVLAAANSNNNTNYNMINSV 195
               +  P  + + +SPS+S E+     S   P   A     +    S   T      + 
Sbjct: 171 AVTVAAVPVVAHI-VSPSNSGEE-QVISSNSSPAAAAAAIGGVVGGGSLQRT--TSCTTA 230

Query: 196 TALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQN 232
             L E+NERLR++N  L  E+  ++ LY +I   + N
Sbjct: 231 PELVEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of Sgr028600 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-45
Identity = 99/218 (45.41%), Postives = 130/218 (59.63%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +SVPAPFLSKTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAYNHHLQQHSSLGVT 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK               S +  T
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRK---------------SVIAST 126

Query: 137 GPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVAGGFSVLAAANSNNNTNYNMINSVTA 196
                   S    +    D+ G+   S PG  +  G                ++ N V  
Sbjct: 127 AGKCVVVGSPSESNSGGGDDHGSSSTSSPGSSKNPG----------------SVENMVAD 186

Query: 197 LSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVK 235
           LS +NE+L+R N+ L SELA  +K  ++++ F+  H+K
Sbjct: 187 LSGENEKLKRENNNLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Sgr028600 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 169.5 bits (428), Expect = 5.1e-42
Identity = 91/217 (41.94%), Postives = 128/217 (58.99%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S +S+P PFL+KT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVAYNHHLQQHSSLGVT 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK     +   H         V 
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK-----ITTTHQT-------VV 135

Query: 137 GPSFFPFSSRVSISPSDSDEQGNWCDSPPGPPRVAGGFSVLAAANSNNNTNYNMINSVTA 196
            PS    +  + +SPS+S E  N        P  +  +        N   +  ++     
Sbjct: 136 APSSEQRNQTMVVSPSNSGEDNNNNQVMSSSP--SSWYCHQTKTTGNGGLSVELL----- 195

Query: 197 LSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHV 234
             E+NE+LR  N  L  EL  M+ + ++I   + N+V
Sbjct: 196 --EENEKLRSQNIQLNRELTQMKSICDNIYSLMSNYV 211

BLAST of Sgr028600 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-39
Identity = 71/99 (71.72%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 20  SVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           S+P PFLSKTY +V+DP+TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ 119
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQ 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029334.14.4e-16080.72Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_022962526.14.4e-16080.72heat stress transcription factor B-4-like [Cucurbita moschata][more]
KAG6598364.15.7e-16080.51Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023545868.16.9e-15880.21heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo][more]
XP_022997563.12.6e-15779.08heat stress transcription factor B-4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C6353.3e-8653.13Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q10KX88.2e-6957.87Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q7XHZ09.0e-6852.42Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U942.7e-6443.51Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R82.9e-5845.10Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A6J1HDI02.1e-16080.72heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1KEA41.3e-15779.08heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0LKU25.9e-15577.81HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 ... [more]
A0A1S3BAP28.6e-15477.60heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=... [more]
A0A5A7V5511.1e-15377.34Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G46264.12.3e-8753.13heat shock transcription factor B4 [more]
AT4G11660.11.8e-4747.00winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.11.3e-4545.41heat shock factor 4 [more]
AT5G62020.15.1e-4241.94heat shock transcription factor B2A [more]
AT4G17750.11.4e-3971.72heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 194..214
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..366
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 63..75
score: 78.68
coord: 76..88
score: 84.62
coord: 25..48
score: 47.26
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 1.2E-60
score: 217.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 2.9E-32
score: 111.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.4E-37
score: 130.4
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..366
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr028600.1Sgr028600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding