Sgr028580 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr028580
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	calmodulin-like
Location	tig00153204: 2490639 .. 2490899 (-)
RNA-Seq Expression	Sgr028580
Synteny	Sgr028580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCATGCAGCAAACAGGCTACAGTTCTGTAGGGAGGACATGAAGGAGATCTTCAGAGAGCACGACCTCGACGGAGATGGTCTCTTGTGCATGAGTGAGCTGGTAAAGGCCTTCGGGTTCCTCGGCAGCATTCTCCCTTTCTACAAGGCTCACTACGGATTGGCTCATGCCGATGCTGATGGAGATGGCTTCATTTGCGAGGACGAGCTCGACAAGCTTGTTGACTTTGCTCAGAGGTTTCAGCACAAGAAGCACTGA

mRNA sequence

ATGTCTCATGCAGCAAACAGGCTACAGTTCTGTAGGGAGGACATGAAGGAGATCTTCAGAGAGCACGACCTCGACGGAGATGGTCTCTTGTGCATGAGTGAGCTGGTAAAGGCCTTCGGGTTCCTCGGCAGCATTCTCCCTTTCTACAAGGCTCACTACGGATTGGCTCATGCCGATGCTGATGGAGATGGCTTCATTTGCGAGGACGAGCTCGACAAGCTTGTTGACTTTGCTCAGAGGTTTCAGCACAAGAAGCACTGA

Coding sequence (CDS)

ATGTCTCATGCAGCAAACAGGCTACAGTTCTGTAGGGAGGACATGAAGGAGATCTTCAGAGAGCACGACCTCGACGGAGATGGTCTCTTGTGCATGAGTGAGCTGGTAAAGGCCTTCGGGTTCCTCGGCAGCATTCTCCCTTTCTACAAGGCTCACTACGGATTGGCTCATGCCGATGCTGATGGAGATGGCTTCATTTGCGAGGACGAGCTCGACAAGCTTGTTGACTTTGCTCAGAGGTTTCAGCACAAGAAGCACTGA

Protein sequence

MSHAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGFICEDELDKLVDFAQRFQHKKH

Homology

[Close]

BLAST of Sgr028580 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 141.4 bits (355), Expect = 3.8e-30
Identity = 65/85 (76.47%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MSHAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADA 60
          MS AA RLQ  RED+KEIFREHD+DGDG L M+E+VKAFG  GSILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFICEDELDKLVDFAQRFQHKK 86
          +GDGFI E+ELDKLVD+AQ+FQHK+
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of Sgr028580 vs. NCBI nr
Match: KAA0060892.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 141.4 bits (355), Expect = 3.8e-30
Identity = 63/85 (74.12%), Postives = 77/85 (90.59%), Query Frame = 0


Query: 1  MSHAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADA 60
          MS AA++LQ  RED+KEIFREHD+DGDG L +SE+VKAFGF GSILPFYKAHYG+AHAD 
Sbjct: 1  MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFICEDELDKLVDFAQRFQHKK 86
          +GDG+I E+ELDKLVD+A++FQHK+
Sbjct: 61 NGDGYIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of Sgr028580 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 2.7e-28
Identity = 61/81 (75.31%), Postives = 72/81 (88.89%), Query Frame = 0


Query: 5   ANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDG 64
           + RLQ  RED+KEIFREHD+DGDG L M+E+VKAFG  GSILPFYKAHYG+AHAD +GDG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  FICEDELDKLVDFAQRFQHKK 86
           FI E+ELDKLVD+AQ+FQHK+
Sbjct: 146 FIEEEELDKLVDYAQKFQHKR 166

[Close]

BLAST of Sgr028580 vs. NCBI nr
Match: KGN62525.1 (hypothetical protein Csa_022453 [Cucumis sativus])

HSP 1 Score: 121.7 bits (304), Expect = 3.1e-24
Identity = 52/80 (65.00%), Postives = 67/80 (83.75%), Query Frame = 0


Query: 6  NRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGF 65
          ++  F RE+MKEIFREHD+DGDG L +SEL+KA GFLG  +PFYKAHYG+A+ D +GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 ICEDELDKLVDFAQRFQHKK 86
          I EDELDKL+D+A+RFQ ++
Sbjct: 67 ISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of Sgr028580 vs. NCBI nr
Match: KAA0060886.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 118.6 bits (296), Expect = 2.6e-23
Identity = 50/78 (64.10%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 3  HAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADG 62
          H  ++  F RE+MKEIFREHD+DGDG L + EL+KA GFLG  +PFYKAHYG+A++D +G
Sbjct: 4  HGVSKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSDENG 63

Query: 63 DGFICEDELDKLVDFAQR 81
          DG I EDELDKL+D+A+R
Sbjct: 64 DGLISEDELDKLIDYAER 81

[Close]

BLAST of Sgr028580 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.9e-05
Identity = 27/62 (43.55%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 13  EDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGFICEDELD 72
           E+M+ +FR+ D +GDG +  SEL   F  LG      +    +A ADADGDGFI  DE  
Sbjct: 39  EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFA 98

Query: 73  KL 75
            L
Sbjct: 99  AL 100

[Close]

BLAST of Sgr028580 vs. ExPASy Swiss-Prot
Match: Q9S744 (Calmodulin-like protein 9 OS=Arabidopsis thaliana OX=3702 GN=CML9 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.6e-04
Identity = 22/63 (34.92%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 13  EDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGFICEDELD 72
           +++ E+FR  D DGDGL+   EL +    +G  +   +A + +  AD DGDGF+   E  
Sbjct: 84  DELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVREADLDGDGFLSFHEFS 143

Query: 73  KLV 76
           K++
Sbjct: 144 KMM 146

[Close]

BLAST of Sgr028580 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.3e-04
Identity = 22/64 (34.38%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 14  DMKEIFREHDLDGDGLLCMSELVKAFGFLG-SILPFYKAHYGLAHADADGDGFICEDELD 73
           ++KE F   D D DGL+   EL++ F  +G            +A  D DGDGF+C  E  
Sbjct: 143 ELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVDEDGDGFVCFTEFS 202

Query: 74  KLVD 77
           +++D
Sbjct: 203 RMMD 206

[Close]

BLAST of Sgr028580 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-30
Identity = 63/85 (74.12%), Postives = 77/85 (90.59%), Query Frame = 0


Query: 1  MSHAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADA 60
          MS AA++LQ  RED+KEIFREHD+DGDG L +SE+VKAFGF GSILPFYKAHYG+AHAD 
Sbjct: 1  MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFICEDELDKLVDFAQRFQHKK 86
          +GDG+I E+ELDKLVD+A++FQHK+
Sbjct: 61 NGDGYIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of Sgr028580 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-30
Identity = 65/85 (76.47%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MSHAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADA 60
          MS AA RLQ  RED+KEIFREHD+DGDG L M+E+VKAFG  GSILPFYKAHYG+AHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGFICEDELDKLVDFAQRFQHKK 86
          +GDGFI E+ELDKLVD+AQ+FQHK+
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of Sgr028580 vs. ExPASy TrEMBL
Match: A0A0A0LKX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-24
Identity = 52/80 (65.00%), Postives = 67/80 (83.75%), Query Frame = 0


Query: 6  NRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGF 65
          ++  F RE+MKEIFREHD+DGDG L +SEL+KA GFLG  +PFYKAHYG+A+ D +GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 ICEDELDKLVDFAQRFQHKK 86
          I EDELDKL+D+A+RFQ ++
Sbjct: 67 ISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of Sgr028580 vs. ExPASy TrEMBL
Match: A0A5A7V2Z9 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00190 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-23
Identity = 50/78 (64.10%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 3  HAANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADG 62
          H  ++  F RE+MKEIFREHD+DGDG L + EL+KA GFLG  +PFYKAHYG+A++D +G
Sbjct: 4  HGVSKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSDENG 63

Query: 63 DGFICEDELDKLVDFAQR 81
          DG I EDELDKL+D+A+R
Sbjct: 64 DGLISEDELDKLIDYAER 81

[Close]

BLAST of Sgr028580 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.8e-22
Identity = 51/81 (62.96%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 5   ANRLQFCREDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDG 64
           A +++F R  +KEIF EHD+DGDG L +SEL+KAF FLGS++P YKAHYGLA AD DGDG
Sbjct: 68  APKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDG 127

Query: 65  FICEDELDKLVDFAQRFQHKK 86
            I E+ELDKLVD+A++   +K
Sbjct: 128 LISEEELDKLVDYAEKGSDRK 148

[Close]

BLAST of Sgr028580 vs. TAIR 10
Match: AT3G51920.1 (calmodulin 9 )

HSP 1 Score: 46.6 bits (109), Expect = 1.2e-05
Identity = 22/63 (34.92%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 13  EDMKEIFREHDLDGDGLLCMSELVKAFGFLGSILPFYKAHYGLAHADADGDGFICEDELD 72
           +++ E+FR  D DGDGL+   EL +    +G  +   +A + +  AD DGDGF+   E  
Sbjct: 84  DELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVREADLDGDGFLSFHEFS 143

Query: 73  KLV 76
           K++
Sbjct: 144 KMM 146

[Close]

BLAST of Sgr028580 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.7 bits (104), Expect = 4.5e-05
Identity = 22/64 (34.38%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 14  DMKEIFREHDLDGDGLLCMSELVKAFGFLG-SILPFYKAHYGLAHADADGDGFICEDELD 73
           ++KE F   D D DGL+   EL++ F  +G            +A  D DGDGF+C  E  
Sbjct: 143 ELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVDEDGDGFVCFTEFS 202

Query: 74  KLVD 77
           +++D
Sbjct: 203 RMMD 206

[Close]

BLAST of Sgr028580 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.2e-04
Identity = 21/72 (29.17%), Postives = 38/72 (52.78%), Query Frame = 0


Query: 13  EDMKEIFREHDLDGDGLLCMSELVKAFGFLG-SILPFYKAHYGLAHADADGDGFICEDEL 72
           E+++E+F   D+D +G +   EL + FG +G       +    +A  D +GDGF+C D+ 
Sbjct: 144 EELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRMIATVDGNGDGFVCFDDF 203

Query: 73  DKLVDFAQRFQH 84
            +++  A    H
Sbjct: 204 CRMMVPAMNDHH 215

The following BLAST results are available for this feature:

BLAST of Sgr028580 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62516.1	3.8e-30	76.47	hypothetical protein Csa_022521 [Cucumis sativus]	[more]
KAA0060892.1	3.8e-30	74.12	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
XP_004152805.1	2.7e-28	75.31	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
KGN62525.1	3.1e-24	65.00	hypothetical protein Csa_022453 [Cucumis sativus]	[more]
KAA0060886.1	2.6e-23	64.10	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]

BLAST of Sgr028580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	1.9e-05	43.55	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9S744	1.6e-04	34.92	Calmodulin-like protein 9 OS=Arabidopsis thaliana OX=3702 GN=CML9 PE=1 SV=1	[more]
Q9SS31	6.3e-04	34.38	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]

BLAST of Sgr028580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYC0	1.8e-30	74.12	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LRC1	1.8e-30	76.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1	1.5e-24	65.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1	[more]
A0A5A7V2Z9	1.3e-23	64.10	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CSH1	1.8e-22	62.96	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Sgr028580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51920.1	1.2e-05	34.92	calmodulin 9	[more]
AT3G10190.1	4.5e-05	34.38	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	2.2e-04	29.17	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 58..75 e-value: 0.0017 score: 17.7
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 14..42 e-value: 4.0E-6 score: 26.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 10..45 score: 12.142296
None	No IPR available	GENE3D	1.10.238.10		coord: 1..84 e-value: 8.3E-10 score: 40.8
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 59..71
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr028580.1	Sgr028580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding