Sgr028528 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028528 vs. NCBI nr
Match: XP_022144232.1 (5'-nucleotidase domain-containing protein 4 isoform X3 [Momordica charantia])

HSP 1 Score: 1018.8 bits (2633), Expect = 2.1e-293
Identity = 507/561 (90.37%), Postives = 523/561 (93.23%), Query Frame = 0

Query: 118 PNAYLNAPCEFPG---DRVRAIDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVE 177
           PN ++  P          +RAIDAMGENLGTVMKDKR +  DVI SE SQ PCIWSSS E
Sbjct: 32  PNCFIRQPKRIHTLGLSHLRAIDAMGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAE 91

Query: 178 CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE 237
           CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE
Sbjct: 92  CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE 151

Query: 238 LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDS 297
           LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDS
Sbjct: 152 LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDS 211

Query: 298 FDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGT 357
           FDEPDYALIDTLFSLAEAYLFAQLVDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGT
Sbjct: 212 FDEPDYALIDTLFSLAEAYLFAQLVDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGT 271

Query: 358 LKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQ 417
           LKQMVAK+PKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQ
Sbjct: 272 LKQMVAKEPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQ 331

Query: 418 KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA- 477
           KCNFDWL+YFDVVITGSAKPGFFH+DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA 
Sbjct: 332 KCNFDWLQYFDVVITGSAKPGFFHEDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAF 391

Query: 478 -PMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE 537
            PMKVDKSCRVFQGGNV+HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE
Sbjct: 392 SPMKVDKSCRVFQGGNVKHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE 451

Query: 538 LEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLE 597
           LEREV+LL ELR TRKQLQ+LRNERDVIEDKIHHLKW LKFEDVEADEKE LST+  QLE
Sbjct: 452 LEREVELLSELRNTRKQLQLLRNERDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLE 511

Query: 598 SEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS 657
           SEREQVRL HQE LR+CH++FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS
Sbjct: 512 SEREQVRLSHQEVLRECHQQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS 571

Query: 658 PDKYYRPSEDFMPHEFGIVPL 673
           PDKYYRPSEDFMPHEFGI+PL
Sbjct: 572 PDKYYRPSEDFMPHEFGIIPL 592

BLAST of Sgr028528 vs. NCBI nr
Match: XP_011649623.1 (5'-nucleotidase domain-containing protein 4 isoform X4 [Cucumis sativus] >KGN62829.2 hypothetical protein Csa_022211 [Cucumis sativus])

HSP 1 Score: 1017.7 bits (2630), Expect = 4.6e-293
Identity = 507/590 (85.93%), Postives = 533/590 (90.34%), Query Frame = 0

Query: 90  PTSVASISVSDSLLTLTIFLPTPGLAFPPNAYLNAPCEFPG---DRVRAIDAMGENLGTV 149
           P    S  +S   L  T  L +PG    PN+++  P        +++R IDAMGENLGTV
Sbjct: 4   PRRQVSFCLSLFSLEFTYPLSSPGFLGIPNSFIRKPKRIHTLGLNQLRPIDAMGENLGTV 63

Query: 150 MKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLA 209
           +KDKRTHS DV   E  Q PCIWSS+ ECKI IGKQIFCNRSLNMKNIVAVGFDMDYTLA
Sbjct: 64  LKDKRTHSLDVTHVEDGQEPCIWSSAEECKIDIGKQIFCNRSLNMKNIVAVGFDMDYTLA 123

Query: 210 QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHKY 269
           QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDK+RGNILKMDRHKY
Sbjct: 124 QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKY 183

Query: 270 VKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP 329
           VKVAYHGFKELSKDDKV+TYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP
Sbjct: 184 VKVAYHGFKELSKDDKVDTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP 243

Query: 330 GKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRS 389
            KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDP+RYIN DT+I+PMLKMLRDSGRS
Sbjct: 244 DKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPERYINVDTTILPMLKMLRDSGRS 303

Query: 390 TFLVTNSLWDYTNVVMNFLCN--LLDGDQKCNFDWLRYFDVVITGSAKPGFFHDDNRANL 449
           TFLVTNSLWDYTNVVMNFLCN    DGDQKCNFDWL+YFDVVITGSAKPGFFHDDNRANL
Sbjct: 304 TFLVTNSLWDYTNVVMNFLCNSSTPDGDQKCNFDWLQYFDVVITGSAKPGFFHDDNRANL 363

Query: 450 FEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVRHLHKLLSIESSSQ 509
           FEVE KSGMLLNTDNGSPMPQVG  S+A  P K+ KSCRVFQGGNVRHLHKLLSIESSSQ
Sbjct: 364 FEVEAKSGMLLNTDNGSPMPQVGMASIALSPTKLSKSCRVFQGGNVRHLHKLLSIESSSQ 423

Query: 510 VLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQLQILRNERDVIEDK 569
           VLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LLWELRKTRKQLQ LRNERD IED+
Sbjct: 424 VLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQQLRNERDAIEDQ 483

Query: 570 IHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCHRKFHGVWGQLMKT 629
           IHHLKWSLKFED+EAD+K+RLSTE  QLE EREQVRL HQ+ALR+CHRKFHGVWGQLMKT
Sbjct: 484 IHHLKWSLKFEDIEADDKDRLSTELHQLEGEREQVRLSHQDALRECHRKFHGVWGQLMKT 543

Query: 630 GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVPL 673
           GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI+PL
Sbjct: 544 GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIPL 593

BLAST of Sgr028528 vs. NCBI nr
Match: XP_022144230.1 (5'-nucleotidase domain-containing protein 4 isoform X1 [Momordica charantia])

HSP 1 Score: 1017.3 bits (2629), Expect = 6.1e-293
Identity = 504/543 (92.82%), Postives = 518/543 (95.40%), Query Frame = 0

Query: 133 VRAIDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMK 192
           +RAIDAMGENLGTVMKDKR +  DVI SE SQ PCIWSSS ECKIGIGKQIFCNRSLNMK
Sbjct: 62  LRAIDAMGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAECKIGIGKQIFCNRSLNMK 121

Query: 193 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 252
           NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD
Sbjct: 122 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 181

Query: 253 KKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEA 312
           KKRGNILKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDSFDEPDYALIDTLFSLAEA
Sbjct: 182 KKRGNILKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEA 241

Query: 313 YLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTS 372
           YLFAQLVDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAK+PKRYINEDTS
Sbjct: 242 YLFAQLVDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKEPKRYINEDTS 301

Query: 373 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQKCNFDWLRYFDVVITGSA 432
           IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQKCNFDWL+YFDVVITGSA
Sbjct: 302 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQKCNFDWLQYFDVVITGSA 361

Query: 433 KPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVR 492
           KPGFFH+DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA  PMKVDKSCRVFQGGNV+
Sbjct: 362 KPGFFHEDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAFSPMKVDKSCRVFQGGNVK 421

Query: 493 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQL 552
           HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LL ELR TRKQL
Sbjct: 422 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLSELRNTRKQL 481

Query: 553 QILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCH 612
           Q+LRNERDVIEDKIHHLKW LKFEDVEADEKE LST+  QLESEREQVRL HQE LR+CH
Sbjct: 482 QLLRNERDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLESEREQVRLSHQEVLRECH 541

Query: 613 RKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 672
           ++FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI
Sbjct: 542 QQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 601

BLAST of Sgr028528 vs. NCBI nr
Match: XP_011649622.1 (5'-nucleotidase domain-containing protein 4 isoform X3 [Cucumis sativus])

HSP 1 Score: 1017.3 bits (2629), Expect = 6.1e-293
Identity = 507/591 (85.79%), Postives = 533/591 (90.19%), Query Frame = 0

Query: 90  PTSVASISVSDSLLTLTIFLPTPGLAFPPNAYLNAPCEFPG----DRVRAIDAMGENLGT 149
           P    S  +S   L  T  L +PG    PN+++  P         +++R IDAMGENLGT
Sbjct: 4   PRRQVSFCLSLFSLEFTYPLSSPGFLGIPNSFIRKPKRIHTLAGLNQLRPIDAMGENLGT 63

Query: 150 VMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMKNIVAVGFDMDYTL 209
           V+KDKRTHS DV   E  Q PCIWSS+ ECKI IGKQIFCNRSLNMKNIVAVGFDMDYTL
Sbjct: 64  VLKDKRTHSLDVTHVEDGQEPCIWSSAEECKIDIGKQIFCNRSLNMKNIVAVGFDMDYTL 123

Query: 210 AQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHK 269
           AQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDK+RGNILKMDRHK
Sbjct: 124 AQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHK 183

Query: 270 YVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNN 329
           YVKVAYHGFKELSKDDKV+TYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNN
Sbjct: 184 YVKVAYHGFKELSKDDKVDTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNN 243

Query: 330 PGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGR 389
           P KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDP+RYIN DT+I+PMLKMLRDSGR
Sbjct: 244 PDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPERYINVDTTILPMLKMLRDSGR 303

Query: 390 STFLVTNSLWDYTNVVMNFLCN--LLDGDQKCNFDWLRYFDVVITGSAKPGFFHDDNRAN 449
           STFLVTNSLWDYTNVVMNFLCN    DGDQKCNFDWL+YFDVVITGSAKPGFFHDDNRAN
Sbjct: 304 STFLVTNSLWDYTNVVMNFLCNSSTPDGDQKCNFDWLQYFDVVITGSAKPGFFHDDNRAN 363

Query: 450 LFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVRHLHKLLSIESSS 509
           LFEVE KSGMLLNTDNGSPMPQVG  S+A  P K+ KSCRVFQGGNVRHLHKLLSIESSS
Sbjct: 364 LFEVEAKSGMLLNTDNGSPMPQVGMASIALSPTKLSKSCRVFQGGNVRHLHKLLSIESSS 423

Query: 510 QVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQLQILRNERDVIED 569
           QVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LLWELRKTRKQLQ LRNERD IED
Sbjct: 424 QVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQQLRNERDAIED 483

Query: 570 KIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCHRKFHGVWGQLMK 629
           +IHHLKWSLKFED+EAD+K+RLSTE  QLE EREQVRL HQ+ALR+CHRKFHGVWGQLMK
Sbjct: 484 QIHHLKWSLKFEDIEADDKDRLSTELHQLEGEREQVRLSHQDALRECHRKFHGVWGQLMK 543

Query: 630 TGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVPL 673
           TGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI+PL
Sbjct: 544 TGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIPL 594

BLAST of Sgr028528 vs. NCBI nr
Match: XP_011649621.1 (5'-nucleotidase domain-containing protein 4 isoform X2 [Cucumis sativus])

HSP 1 Score: 1013.4 bits (2619), Expect = 8.7e-292
Identity = 507/601 (84.36%), Postives = 533/601 (88.69%), Query Frame = 0

Query: 90  PTSVASISVSDSLLTLTIFLPTPGLAFPPNAYLNAPCEFPG--------------DRVRA 149
           P    S  +S   L  T  L +PG    PN+++  P                   +++R 
Sbjct: 4   PRRQVSFCLSLFSLEFTYPLSSPGFLGIPNSFIRKPKRIHTLGSSFSLLFHLSGLNQLRP 63

Query: 150 IDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMKNIV 209
           IDAMGENLGTV+KDKRTHS DV   E  Q PCIWSS+ ECKI IGKQIFCNRSLNMKNIV
Sbjct: 64  IDAMGENLGTVLKDKRTHSLDVTHVEDGQEPCIWSSAEECKIDIGKQIFCNRSLNMKNIV 123

Query: 210 AVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKR 269
           AVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDK+R
Sbjct: 124 AVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKRR 183

Query: 270 GNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLF 329
           GNILKMDRHKYVKVAYHGFKELSKDDKV+TYGNTLVRDSFDEPDYALIDTLFSLAEAYLF
Sbjct: 184 GNILKMDRHKYVKVAYHGFKELSKDDKVDTYGNTLVRDSFDEPDYALIDTLFSLAEAYLF 243

Query: 330 AQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTSIVP 389
           AQLVDFKDNNP KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDP+RYIN DT+I+P
Sbjct: 244 AQLVDFKDNNPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPERYINVDTTILP 303

Query: 390 MLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN--LLDGDQKCNFDWLRYFDVVITGSAKP 449
           MLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN    DGDQKCNFDWL+YFDVVITGSAKP
Sbjct: 304 MLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSTPDGDQKCNFDWLQYFDVVITGSAKP 363

Query: 450 GFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVRHL 509
           GFFHDDNRANLFEVE KSGMLLNTDNGSPMPQVG  S+A  P K+ KSCRVFQGGNVRHL
Sbjct: 364 GFFHDDNRANLFEVEAKSGMLLNTDNGSPMPQVGMASIALSPTKLSKSCRVFQGGNVRHL 423

Query: 510 HKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQLQI 569
           HKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LLWELRKTRKQLQ 
Sbjct: 424 HKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQQ 483

Query: 570 LRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCHRK 629
           LRNERD IED+IHHLKWSLKFED+EAD+K+RLSTE  QLE EREQVRL HQ+ALR+CHRK
Sbjct: 484 LRNERDAIEDQIHHLKWSLKFEDIEADDKDRLSTELHQLEGEREQVRLSHQDALRECHRK 543

Query: 630 FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVP 673
           FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI+P
Sbjct: 544 FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIP 603

BLAST of Sgr028528 vs. ExPASy Swiss-Prot
Match: Q3V1L4 (Cytosolic purine 5'-nucleotidase OS=Mus musculus OX=10090 GN=Nt5c2 PE=1 SV=2)

HSP 1 Score: 244.6 bits (623), Expect = 3.2e-63
Identity = 164/496 (33.06%), Postives = 267/496 (53.83%), Query Frame = 0

Query: 182 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFD 241
           ++F NRSL M+ I   GFDMDYTLA YK   +ESL +E T+ +LV  +GY +ELL++++D
Sbjct: 34  RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93

Query: 242 WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYA 301
             +  RGLV D   GN+LK+D +  + V  HGF  +   +  E Y N  ++   D   + 
Sbjct: 94  STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153

Query: 302 LIDTLFSLAEAYLFAQLVDFKDNNPGKVPED---------IDYARMYKDVRAAVDLCHRD 361
           +++TLF+L E YL A LVDF  N P     D         + Y  M++DVR AVD  H  
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213

Query: 362 GTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDGD 421
           G+LK+   ++ ++Y+ +D  +  +L  +++ G+  FL TNS + YT+ +M +L +   G 
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273

Query: 422 Q--KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTS 481
           +    +  W  YFD+++  + KP FF +     L +V+ K+G L          ++G T 
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKL----------KIG-TY 333

Query: 482 LAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVP 541
             P+   +   V+ GG+   +  LL  +    +LY+GDHI+GDIL+SKK  GWRT LV+P
Sbjct: 334 TGPL---QHGIVYSGGSSDTICDLLGAK-GKDILYIGDHIFGDILKSKKRQGWRTFLVIP 393

Query: 542 ELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQL 601
           EL +E+ +  +     ++LQ L    D+   +++      K  D  ++E+  +S+ QR++
Sbjct: 394 ELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDISSIQRRI 453

Query: 602 ESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLY 661
           +     + +             +G+ G L ++G + + FA QV R+A LY++   NL  Y
Sbjct: 454 KKVTHDMDM------------CYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYY 487

Query: 662 SPDKYYRPSEDFMPHE 667
                +R +   MPHE
Sbjct: 514 PFSYLFRAAHVLMPHE 487

BLAST of Sgr028528 vs. ExPASy Swiss-Prot
Match: P49902 (Cytosolic purine 5'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5C2 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 7.2e-63
Identity = 165/496 (33.27%), Postives = 268/496 (54.03%), Query Frame = 0

Query: 182 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFD 241
           ++F NRSL M+ I   GFDMDYTLA YK   +ESL +E T+ +LV  +GY +ELL++++D
Sbjct: 34  RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93

Query: 242 WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYA 301
             +  RGLV D   GN+LK+D +  + V  HGF  +   +  E Y N  ++   D   + 
Sbjct: 94  STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153

Query: 302 LIDTLFSLAEAYLFAQLVDFKDNNP-------GKVPEDI--DYARMYKDVRAAVDLCHRD 361
           +++TLF+L E YL A LVDF  N P       G    D+   Y  M++DVR AVD  H  
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213

Query: 362 GTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDGD 421
           G+LK+   ++ ++Y+ +D  +  +L  +++ G+  FL TNS + YT+ +M +L +   G 
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273

Query: 422 Q--KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTS 481
           +    +  W  YFD+++  + KP FF +     L +V+ K+G L          ++G T 
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKL----------KIG-TY 333

Query: 482 LAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVP 541
             P+   +   V+ GG+   +  LL  +    +LY+GDHI+GDIL+SKK  GWRT LV+P
Sbjct: 334 TGPL---QHGIVYSGGSSDTICDLLGAK-GKDILYIGDHIFGDILKSKKRQGWRTFLVIP 393

Query: 542 ELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQL 601
           EL +E+ +  +     ++LQ L    D+   +++      K  D  ++E+  +S+ QR++
Sbjct: 394 ELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDISSIQRRI 453

Query: 602 ESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLY 661
           +     + +             +G+ G L ++G + + FA QV R+A LY++   NL  Y
Sbjct: 454 KKVTHDMDM------------CYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYY 487

Query: 662 SPDKYYRPSEDFMPHE 667
                +R +   MPHE
Sbjct: 514 PFSYLFRAAHVLMPHE 487

BLAST of Sgr028528 vs. ExPASy Swiss-Prot
Match: Q5RA22 (Cytosolic purine 5'-nucleotidase OS=Pongo abelii OX=9601 GN=NT5C2 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 7.2e-63
Identity = 165/496 (33.27%), Postives = 268/496 (54.03%), Query Frame = 0

Query: 182 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFD 241
           ++F NRSL M+ I   GFDMDYTLA YK   +ESL +E T+ +LV  +GY +ELL++++D
Sbjct: 34  RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93

Query: 242 WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYA 301
             +  RGLV D   GN+LK+D +  + V  HGF  +   +  E Y N  ++   D   + 
Sbjct: 94  STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153

Query: 302 LIDTLFSLAEAYLFAQLVDFKDNNP-------GKVPEDI--DYARMYKDVRAAVDLCHRD 361
           +++TLF+L E YL A LVDF  N P       G    D+   Y  M++DVR AVD  H  
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213

Query: 362 GTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDGD 421
           G+LK+   ++ ++Y+ +D  +  +L  +++ G+  FL TNS + YT+ +M +L +   G 
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273

Query: 422 Q--KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTS 481
           +    +  W  YFD+++  + KP FF +     L +V+ K+G L          ++G T 
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKL----------KIG-TY 333

Query: 482 LAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVP 541
             P+   +   V+ GG+   +  LL  +    +LY+GDHI+GDIL+SKK  GWRT LV+P
Sbjct: 334 TGPL---QHGIVYSGGSSDTICDLLGAK-GKDILYIGDHIFGDILKSKKRQGWRTFLVIP 393

Query: 542 ELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQL 601
           EL +E+ +  +     ++LQ L    D+   +++      K  D  ++E+  +S+ QR++
Sbjct: 394 ELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDISSIQRRI 453

Query: 602 ESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLY 661
           +     + +             +G+ G L ++G + + FA QV R+A LY++   NL  Y
Sbjct: 454 KKVTHDMDM------------CYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYY 487

Query: 662 SPDKYYRPSEDFMPHE 667
                +R +   MPHE
Sbjct: 514 PFSYLFRAAHVLMPHE 487

BLAST of Sgr028528 vs. ExPASy Swiss-Prot
Match: O46411 (Cytosolic purine 5'-nucleotidase OS=Bos taurus OX=9913 GN=NT5C2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 9.4e-63
Identity = 165/496 (33.27%), Postives = 268/496 (54.03%), Query Frame = 0

Query: 182 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFD 241
           ++F NRSL M+ I   GFDMDYTLA YK   +ESL +E T+ +LV  +GY +ELL++++D
Sbjct: 34  RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93

Query: 242 WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYA 301
             +  RGLV D   GN+LK+D +  + V  HGF  +   +  E Y N  ++   D   + 
Sbjct: 94  STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153

Query: 302 LIDTLFSLAEAYLFAQLVDFKDNNP-------GKVPEDI--DYARMYKDVRAAVDLCHRD 361
           +++TLF+L E YL A LVDF  N P       G    D+   Y  M++DVR AVD  H  
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213

Query: 362 GTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDGD 421
           G+LK+   ++ ++Y+ +D  +  +L  +++ G+  FL TNS + YT+ +M +L +   G 
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273

Query: 422 Q--KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTS 481
           +    +  W  YFD+++  + KP FF +     L +V+ K+G L          ++G T 
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKL----------KIG-TY 333

Query: 482 LAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVP 541
             P+   +   V+ GG+   +  LL  +    +LY+GDHI+GDIL+SKK  GWRT LV+P
Sbjct: 334 TGPL---QHGIVYSGGSSDTVCDLLGAK-GKDILYIGDHIFGDILKSKKRQGWRTFLVIP 393

Query: 542 ELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQL 601
           EL +E+ +  +     ++LQ L    D+   +++      K  D  ++E+  +S+ QR++
Sbjct: 394 ELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDISSIQRRI 453

Query: 602 ESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLY 661
           +     + +             +G+ G L ++G + + FA QV R+A LY++   NL  Y
Sbjct: 454 KKVTHDMDM------------CYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYY 487

Query: 662 SPDKYYRPSEDFMPHE 667
                +R +   MPHE
Sbjct: 514 PFSYLFRAAHVLMPHE 487

BLAST of Sgr028528 vs. ExPASy Swiss-Prot
Match: Q6DKB0 (Cytosolic purine 5'-nucleotidase OS=Xenopus laevis OX=8355 GN=nt5c2 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.9e-61
Identity = 164/497 (33.00%), Postives = 264/497 (53.12%), Query Frame = 0

Query: 182 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFD 241
           ++F NRSL M+ I   GFDMDYTLA YK   +ESL ++ T+ +LV  +GY +ELLN+ +D
Sbjct: 34  RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLV-SIGYPQELLNFVYD 93

Query: 242 WKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLV-RDSFDEPDY 301
             +  RGLV D   GN++K+D +  + V  HGF  +   +  E Y N  + RD  D   +
Sbjct: 94  PTFPTRGLVFDSTYGNLIKVDAYGNILVCAHGFNFMRGLETREQYPNKFIQRDDTDR--F 153

Query: 302 ALIDTLFSLAEAYLFAQLVDFKDN-------NPGKVPEDI--DYARMYKDVRAAVDLCHR 361
            +++TLF+L E YL A LVDF  N         G    D+   +  M++DVR AVD  H 
Sbjct: 154 YILNTLFNLPETYLLACLVDFFTNCDRYSSCETGFKDGDLFMSFRSMFQDVRDAVDWVHY 213

Query: 362 DGTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDG 421
            G+LK+   ++  +Y+ +D+ +  +L  + + G+  FLVTNS + YT+ +M +L +L  G
Sbjct: 214 KGSLKEKTVENLPKYVVKDSKLPLLLSRMNEVGK-VFLVTNSDYKYTHKIMTYLFDLPHG 273

Query: 422 DQ--KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNT 481
            +    +  W  YFD+++  + KP FF +              +L   D  +   ++G T
Sbjct: 274 PKPDSSHRVWQSYFDLILVDARKPLFFGEGT------------VLRQVDTNTGKLKIG-T 333

Query: 482 SLAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVV 541
              P+   +   V+ GG+   L  LL  +    +LY+GDHI+GDIL+SKK  GWRT LV+
Sbjct: 334 YTGPL---QHGIVYSGGSSDILCDLLGAK-GKDILYIGDHIFGDILKSKKRQGWRTFLVI 393

Query: 542 PELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQ 601
           PEL +E+ +  +     ++LQ L    D+   +++      K  D  ++E+  +S+ QR+
Sbjct: 394 PELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDISSIQRR 453

Query: 602 LESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSL 661
           ++     + +             +G+ G L ++G + + FA QV R+A LY++   NL  
Sbjct: 454 VKKVTHDMDM------------CYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLY 487

Query: 662 YSPDKYYRPSEDFMPHE 667
           Y     +R +   MPHE
Sbjct: 514 YPFSYLFRAAHVLMPHE 487

BLAST of Sgr028528 vs. ExPASy TrEMBL
Match: A0A6J1CT34 (5'-nucleotidase domain-containing protein 4 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013967 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 1.0e-293
Identity = 507/561 (90.37%), Postives = 523/561 (93.23%), Query Frame = 0

Query: 118 PNAYLNAPCEFPG---DRVRAIDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVE 177
           PN ++  P          +RAIDAMGENLGTVMKDKR +  DVI SE SQ PCIWSSS E
Sbjct: 32  PNCFIRQPKRIHTLGLSHLRAIDAMGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAE 91

Query: 178 CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE 237
           CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE
Sbjct: 92  CKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEE 151

Query: 238 LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDS 297
           LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDS
Sbjct: 152 LLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDS 211

Query: 298 FDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGT 357
           FDEPDYALIDTLFSLAEAYLFAQLVDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGT
Sbjct: 212 FDEPDYALIDTLFSLAEAYLFAQLVDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGT 271

Query: 358 LKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQ 417
           LKQMVAK+PKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQ
Sbjct: 272 LKQMVAKEPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQ 331

Query: 418 KCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA- 477
           KCNFDWL+YFDVVITGSAKPGFFH+DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA 
Sbjct: 332 KCNFDWLQYFDVVITGSAKPGFFHEDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAF 391

Query: 478 -PMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE 537
            PMKVDKSCRVFQGGNV+HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE
Sbjct: 392 SPMKVDKSCRVFQGGNVKHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPE 451

Query: 538 LEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLE 597
           LEREV+LL ELR TRKQLQ+LRNERDVIEDKIHHLKW LKFEDVEADEKE LST+  QLE
Sbjct: 452 LEREVELLSELRNTRKQLQLLRNERDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLE 511

Query: 598 SEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS 657
           SEREQVRL HQE LR+CH++FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS
Sbjct: 512 SEREQVRLSHQEVLRECHQQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYS 571

Query: 658 PDKYYRPSEDFMPHEFGIVPL 673
           PDKYYRPSEDFMPHEFGI+PL
Sbjct: 572 PDKYYRPSEDFMPHEFGIIPL 592

BLAST of Sgr028528 vs. ExPASy TrEMBL
Match: A0A6J1CR23 (5'-nucleotidase domain-containing protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013967 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.9e-293
Identity = 504/543 (92.82%), Postives = 518/543 (95.40%), Query Frame = 0

Query: 133 VRAIDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMK 192
           +RAIDAMGENLGTVMKDKR +  DVI SE SQ PCIWSSS ECKIGIGKQIFCNRSLNMK
Sbjct: 62  LRAIDAMGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAECKIGIGKQIFCNRSLNMK 121

Query: 193 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 252
           NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD
Sbjct: 122 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 181

Query: 253 KKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEA 312
           KKRGNILKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDSFDEPDYALIDTLFSLAEA
Sbjct: 182 KKRGNILKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEA 241

Query: 313 YLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTS 372
           YLFAQLVDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAK+PKRYINEDTS
Sbjct: 242 YLFAQLVDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKEPKRYINEDTS 301

Query: 373 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQKCNFDWLRYFDVVITGSA 432
           IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQKCNFDWL+YFDVVITGSA
Sbjct: 302 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQKCNFDWLQYFDVVITGSA 361

Query: 433 KPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVR 492
           KPGFFH+DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA  PMKVDKSCRVFQGGNV+
Sbjct: 362 KPGFFHEDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAFSPMKVDKSCRVFQGGNVK 421

Query: 493 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQL 552
           HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LL ELR TRKQL
Sbjct: 422 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLSELRNTRKQL 481

Query: 553 QILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCH 612
           Q+LRNERDVIEDKIHHLKW LKFEDVEADEKE LST+  QLESEREQVRL HQE LR+CH
Sbjct: 482 QLLRNERDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLESEREQVRLSHQEVLRECH 541

Query: 613 RKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 672
           ++FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI
Sbjct: 542 QQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 601

BLAST of Sgr028528 vs. ExPASy TrEMBL
Match: A0A1S3BBU8 (5'-nucleotidase domain-containing protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487969 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 6.1e-291
Identity = 503/590 (85.25%), Postives = 531/590 (90.00%), Query Frame = 0

Query: 90  PTSVASISVSDSLLTLTIFLPTPGLAFPPNAYLNAPCEFPG---DRVRAIDAMGENLGTV 149
           P    S  +S   L  T  L +P     PN+++  P        +++R IDAMGENLGTV
Sbjct: 4   PRRQVSFCLSLFSLEFTYPLSSPSFLGIPNSFIRKPKRIHTLGLNQLRPIDAMGENLGTV 63

Query: 150 MKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLA 209
           +K+KRTHS DV  +E  Q PCIWSS  ECKI IGKQIFCNRSLNMKNIVAVGFDMDYTLA
Sbjct: 64  LKEKRTHSLDVTHTEDGQEPCIWSSPEECKIDIGKQIFCNRSLNMKNIVAVGFDMDYTLA 123

Query: 210 QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHKY 269
           QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHKY
Sbjct: 124 QYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHKY 183

Query: 270 VKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP 329
           VKVAYHGFKE+SKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP
Sbjct: 184 VKVAYHGFKEMSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP 243

Query: 330 GKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRS 389
            KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDP+RYIN DTSI+PMLKMLRDSGRS
Sbjct: 244 DKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPERYINVDTSILPMLKMLRDSGRS 303

Query: 390 TFLVTNSLWDYTNVVMNFLCN--LLDGDQKCNFDWLRYFDVVITGSAKPGFFHDDNRANL 449
           TFLVTNSLWDYTNVVMNFLCN    DGDQK NFDWL+YFDVVITGSAKPGFFHDDNRANL
Sbjct: 304 TFLVTNSLWDYTNVVMNFLCNSSASDGDQKRNFDWLQYFDVVITGSAKPGFFHDDNRANL 363

Query: 450 FEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVRHLHKLLSIESSSQ 509
           FEVE KSGMLLNTDNGSPMPQVG  S+A  P K+ KSCRVFQGGNVRHLHKLLSIESSSQ
Sbjct: 364 FEVEAKSGMLLNTDNGSPMPQVGTASMALSPTKLSKSCRVFQGGNVRHLHKLLSIESSSQ 423

Query: 510 VLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQLQILRNERDVIEDK 569
           VLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LLWELRKTRKQLQ+LRNERD IED+
Sbjct: 424 VLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNERDAIEDQ 483

Query: 570 IHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCHRKFHGVWGQLMKT 629
           IHHLKWSLKFED+EADEK++LST   QLE ERE+VRL HQ+ALR+CHR+FHGVWGQLMKT
Sbjct: 484 IHHLKWSLKFEDIEADEKDKLSTGLHQLEGERERVRLSHQDALRECHRQFHGVWGQLMKT 543

Query: 630 GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVPL 673
           GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI+PL
Sbjct: 544 GYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIPL 593

BLAST of Sgr028528 vs. ExPASy TrEMBL
Match: A0A6J1CSS4 (5'-nucleotidase domain-containing protein 4 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111013967 PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.3e-290
Identity = 499/537 (92.92%), Postives = 512/537 (95.34%), Query Frame = 0

Query: 139 MGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMKNIVAVG 198
           MGENLGTVMKDKR +  DVI SE SQ PCIWSSS ECKIGIGKQIFCNRSLNMKNIVAVG
Sbjct: 1   MGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAECKIGIGKQIFCNRSLNMKNIVAVG 60

Query: 199 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNI 258
           FDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNI
Sbjct: 61  FDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNI 120

Query: 259 LKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 318
           LKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQL
Sbjct: 121 LKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 180

Query: 319 VDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTSIVPMLK 378
           VDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAK+PKRYINEDTSIVPMLK
Sbjct: 181 VDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKEPKRYINEDTSIVPMLK 240

Query: 379 MLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQKCNFDWLRYFDVVITGSAKPGFFH 438
           MLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQKCNFDWL+YFDVVITGSAKPGFFH
Sbjct: 241 MLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQKCNFDWLQYFDVVITGSAKPGFFH 300

Query: 439 DDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVRHLHKLL 498
           +DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA  PMKVDKSCRVFQGGNV+HLHKLL
Sbjct: 301 EDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAFSPMKVDKSCRVFQGGNVKHLHKLL 360

Query: 499 SIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQLQILRNE 558
           SIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LL ELR TRKQLQ+LRNE
Sbjct: 361 SIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLSELRNTRKQLQLLRNE 420

Query: 559 RDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCHRKFHGV 618
           RDVIEDKIHHLKW LKFEDVEADEKE LST+  QLESEREQVRL HQE LR+CH++FHGV
Sbjct: 421 RDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLESEREQVRLSHQEVLRECHQQFHGV 480

Query: 619 WGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVPL 673
           WGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI+PL
Sbjct: 481 WGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIPL 537

BLAST of Sgr028528 vs. ExPASy TrEMBL
Match: A0A6J1CSP6 (5'-nucleotidase domain-containing protein 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013967 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 4.0e-290
Identity = 501/543 (92.27%), Postives = 515/543 (94.84%), Query Frame = 0

Query: 133 VRAIDAMGENLGTVMKDKRTHSSDVIDSEISQVPCIWSSSVECKIGIGKQIFCNRSLNMK 192
           +RAIDAMGENLGTVMKDKR +  DVI SE SQ PCIWSSS ECKIGIGKQIFCNRSLNMK
Sbjct: 62  LRAIDAMGENLGTVMKDKRRNYLDVIHSEDSQEPCIWSSSAECKIGIGKQIFCNRSLNMK 121

Query: 193 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 252
           NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD
Sbjct: 122 NIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLD 181

Query: 253 KKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEA 312
           KKRGNILKMDRHKYVKVAYHGFKELSK+DKV TYGNTLVRDSFDEPDYALIDTLFSLAEA
Sbjct: 182 KKRGNILKMDRHKYVKVAYHGFKELSKEDKVGTYGNTLVRDSFDEPDYALIDTLFSLAEA 241

Query: 313 YLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPKRYINEDTS 372
           YLFAQLVDFKD  P KVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAK+PKRYINEDTS
Sbjct: 242 YLFAQLVDFKDKYPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKEPKRYINEDTS 301

Query: 373 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQKCNFDWLRYFDVVITGSA 432
           IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN   DGDQKCNFDWL+YFDVVITGSA
Sbjct: 302 IVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNSSADGDQKCNFDWLQYFDVVITGSA 361

Query: 433 KPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLA--PMKVDKSCRVFQGGNVR 492
           KPGFFH+DNRANLFEVE KSGMLLNTDNGSPMPQVGNTSLA  PMKVDKSCRVFQGGNV+
Sbjct: 362 KPGFFHEDNRANLFEVEAKSGMLLNTDNGSPMPQVGNTSLAFSPMKVDKSCRVFQGGNVK 421

Query: 493 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLWELRKTRKQL 552
           HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREV+LL ELR TRK  
Sbjct: 422 HLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLSELRNTRK-- 481

Query: 553 QILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLRHQEALRDCH 612
            +LRNERDVIEDKIHHLKW LKFEDVEADEKE LST+  QLESEREQVRL HQE LR+CH
Sbjct: 482 -LLRNERDVIEDKIHHLKWFLKFEDVEADEKETLSTKLHQLESEREQVRLSHQEVLRECH 541

Query: 613 RKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 672
           ++FHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI
Sbjct: 542 QQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGI 601

BLAST of Sgr028528 vs. TAIR 10
Match: AT1G75210.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )

HSP 1 Score: 837.0 bits (2161), Expect = 1.0e-242
Identity = 406/513 (79.14%), Postives = 452/513 (88.11%), Query Frame = 0

Query: 165 VPCIWSS-SVECKIGIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIR 224
           VP  WSS     KI I  QIFCNRSLNMKNI+AVGFDMDYTLAQYK ETFESLAY+GT+R
Sbjct: 130 VPHEWSSPEGGKKIDISNQIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYDGTVR 189

Query: 225 KLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKV 284
           KLVYDLGY  ELL W+FDW YMVRGLVLDKKRGNILKMDRHKYVKVAYHGF+ELSK+DKV
Sbjct: 190 KLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRELSKEDKV 249

Query: 285 ETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPGKVPEDIDYARMYKDVR 344
           E YG++LVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNP KVP+D+DYARMYKDVR
Sbjct: 250 EIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKVPKDVDYARMYKDVR 309

Query: 345 AAVDLCHRDGTLKQMVAKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMN 404
           AAVDLCHRDGTLKQMVAK+P RYINEDT+IVP++KM+RDSGRSTFLVTNSLWDYTN+VMN
Sbjct: 310 AAVDLCHRDGTLKQMVAKEPNRYINEDTTIVPLIKMIRDSGRSTFLVTNSLWDYTNIVMN 369

Query: 405 FLC--NLLDGDQKCNFDWLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGS 464
           FLC    + G   CNFDWL+YFDVVITGSAKPGFFH+D+RANLFEVEP+SGML+NTDNG+
Sbjct: 370 FLCGGRTVHGPHTCNFDWLQYFDVVITGSAKPGFFHEDSRANLFEVEPQSGMLINTDNGT 429

Query: 465 PMPQVGNTS--LAPMKVDKSCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKK 524
           PM QVG+ S  +     DK CRVFQGGNV HLH LLSIESSSQVLYVGDHIYGDILRSKK
Sbjct: 430 PMAQVGDPSPKILLKSKDKGCRVFQGGNVGHLHSLLSIESSSQVLYVGDHIYGDILRSKK 489

Query: 525 ALGWRTMLVVPELEREVKLLWELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADE 584
            LGWRTMLVVPELE+EV+LLWELR  RK L ++RNERD +EDKIHHL WSLKFED+  + 
Sbjct: 490 ILGWRTMLVVPELEKEVELLWELRNMRKDLILMRNERDSVEDKIHHLNWSLKFEDINENN 549

Query: 585 KERLSTEQRQLESEREQVRLRHQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACL 644
           K  + +  + LES+R++VR  HQ+A R+CH+KFH VWGQLMKTGYQ+SRFAHQVERFACL
Sbjct: 550 KHEMLSALKDLESKRDKVRQSHQQAQRECHQKFHKVWGQLMKTGYQSSRFAHQVERFACL 609

Query: 645 YSSQVTNLSLYSPDKYYRPSEDFMPHEFGIVPL 673
           Y+SQV+NL LYSPDKYYRPSEDFM HEF ++PL
Sbjct: 610 YTSQVSNLRLYSPDKYYRPSEDFMSHEFHLLPL 642

BLAST of Sgr028528 vs. TAIR 10
Match: AT5G48960.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )

HSP 1 Score: 222.6 bits (566), Expect = 9.3e-58
Identity = 158/499 (31.66%), Postives = 259/499 (51.90%), Query Frame = 0

Query: 183 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDW 242
           IFC+R+LN+++I A+G+DMDYTL  Y    +E  AY+  +  L   +G+  + L  +FD 
Sbjct: 165 IFCSRTLNLRSISAIGYDMDYTLMHYNVMAWEGKAYDYCMENL-KSMGFPVDGL--AFDP 224

Query: 243 KYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGNTLVRDSFDEPDYAL 302
           + ++RGL++DK++GN++K DR  YVK A HG K LS     E YG  LV D  ++  +  
Sbjct: 225 ELVIRGLMIDKEKGNLVKADRFGYVKRAMHGTKMLSNKAVSEIYGRELV-DLRNQSRWEF 284

Query: 303 IDTLFSLAEAYLFAQLVDFKDNNPGKVPED---IDYARMYKDVRAAVDLCHRDGTLKQMV 362
           ++T FS++EA  +AQ+VD  D+  G +  D   +DY  +YK V  A+   H +G LK  +
Sbjct: 285 LNTFFSVSEALAYAQMVDRLDD--GFISADLGTLDYKGLYKAVAKALFRAHVEGQLKSEI 344

Query: 363 AKDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCN-LLDGDQKCNFD 422
              P+ ++  D  +   L   +++G+   L+TNS + YT+ +M    N  L  D     D
Sbjct: 345 MSKPELFVEPDPELPLALLDQKEAGKKLLLITNSDYHYTDKMMKHSFNKFLPND----MD 404

Query: 423 WLRYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLAPMKVDK 482
           W   FD+VI  + KP FF   +   L+EV    G+                 + P    +
Sbjct: 405 WRDLFDMVIVSARKPEFFQMSH--PLYEVVTGEGL-----------------MRPCFKAE 464

Query: 483 SCRVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKL 542
           +  ++ GG+ + +   L++    ++LYVGDHIY D+  SK  L WRT L+  ELE E   
Sbjct: 465 TGGLYSGGSAQMIESSLNVH-GDEILYVGDHIYTDVSVSKVHLRWRTALICRELEEEYMA 524

Query: 543 LWELRKTRKQLQILRNERDVIEDKIHHLKWSLK----------FEDVEADEKERLSTEQR 602
           L   R  R++L  L N+++V+ D  + L+ +L+                D++E   T Q+
Sbjct: 525 LIGSRGHREELIELINQKEVVGDLFNQLRLALQRRSKGRPAQTLAATNLDDQELTETMQK 584

Query: 603 QLESEREQVRLRHQEALRDCHRKFHGVWGQLMKTG-YQNSRFAHQVERFACLYSSQVTNL 662
            L    +++  +    L      F+  WG L + G +  S    Q+E++A +Y+S+V+N 
Sbjct: 585 LL-IVMQRLDDKIGLMLETDGELFNKRWGFLSRAGLWDKSHLMRQIEKYADIYTSRVSNF 632

Query: 663 SLYSPDKYYRPSEDFMPHE 667
             Y+P  Y+R  E  + H+
Sbjct: 645 LNYTPFMYFRSQEQSLAHD 632

BLAST of Sgr028528 vs. TAIR 10
Match: AT2G23890.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )

HSP 1 Score: 157.1 bits (396), Expect = 4.8e-38
Identity = 125/487 (25.67%), Postives = 218/487 (44.76%), Query Frame = 0

Query: 183 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDW 242
           I+ N++L + NI   GFD DYTLA Y     +SL Y+   + +V +  Y +    + +D 
Sbjct: 99  IYVNKNLRLDNIQVYGFDYDYTLAHYSSH-LQSLIYDLAKKHMVNEFRYPDVCTQFEYDP 158

Query: 243 KYMVRGLVLDKKRGNILKMDRHKYVKV--AYHGFKELSKDDKVETYGNTLVRDSFDEPDY 302
            + +RGL  DK +G ++K+D    ++    Y G ++LS+ +    YG   +         
Sbjct: 159 TFPIRGLYYDKLKGCLMKLDFFGSIEPDGCYFGRRKLSRKEIESMYGTRHIGRDQARGLV 218

Query: 303 ALIDTLFSLAEAYLFAQLVDFKDNNPGKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVA 362
            L+D  F  +EA L A +V +  +       + D + +Y DV  A+   HR G + + + 
Sbjct: 219 GLMD-FFCFSEACLIADMVQYFVD----AKLEFDASNIYNDVNRAIQHVHRSGLVHRGIL 278

Query: 363 KDPKRYINEDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCNLLDGDQKCNFDWL 422
            DP RY+ ++  ++  L+ML+D G+  FL+TNS +++ +  M F   L++        W 
Sbjct: 279 ADPNRYLLKNGQLLRFLRMLKDKGKKLFLLTNSPYNFVDGGMRF---LMEESFGFGDSWR 338

Query: 423 RYFDVVITGSAKPGFFHDDNRANLFEVEPKSGMLLNTDNGSPMPQVGNTSLAPMKVDKSC 482
             FDVVI  + KP F+  ++    ++ E  +      D   P                  
Sbjct: 339 ELFDVVIAKANKPEFYTSEHPFRCYDSERDNLAFTKVDAFDPK----------------- 398

Query: 483 RVFQGGNVRHLHKLLSIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVKLLW 542
           +V+  G ++   ++       +V+Y GDH++ D LR     GWRT  ++ ELERE+    
Sbjct: 399 KVYYHGCLKSFLEITKWH-GPEVIYFGDHLFSD-LRGPSKAGWRTAAIIHELEREI---- 458

Query: 543 ELRKTRKQLQILRNERDVIEDKIHHLKWSLKFEDVEADEKERLSTEQRQLESEREQVRLR 602
                    QI  ++    E    H+   L           + S   + L  E    R R
Sbjct: 459 ---------QIQNDDSYRFEQAKFHIIQELLGRFHATVSNNQRSEACQSLLDELNNARQR 518

Query: 603 HQEALRDCHRKFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSE 662
            ++ ++    +  G    +  TG Q S F++ + ++A +Y+S+  N  LY P+ +     
Sbjct: 519 ARDTMKQMFNRSFGA-TFVTDTG-QESAFSYHIHQYADVYTSKPENFLLYRPEAWLHVPY 542

Query: 663 D--FMPH 666
           D   MPH
Sbjct: 579 DIKIMPH 542