Sgr028322 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr028322
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAuxin efflux carrier component
Locationtig00153057: 1646214 .. 1651254 (+)
RNA-Seq ExpressionSgr028322
SyntenySgr028322
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTACAGCGGGAGATTTCTACAAGGTGATGTGCGCGATGGTTCCGCTGTACTTCGCAATGCTGGTGGCGTACACCTCGGTGAAGTGGTGGAAGATATTCACGGCGGAGCAATGCGCCGGAATCAACCGCTTTGTGGCTGTGTTCGCTGTGCCTGTGCTTTCCTTCCATTTCATCTCTCAGAACAACCCTTACCAGATGGACACCAAGTTCATATTAGCCGACACTCTTTCCAAGCTCTTCGTGCTCCTTCTGCTCTCTCTCTGGGCCGTCTTCTTCACCGCCGGCAGCCTCGACTGGCTCATCACTCTCTTCTCTCTCGCCACTTTGCCCAACACTCTGGTCATGGGTATCCCTCTCCTCAACGCCATGTATGGCGATTCCACTCAGTCCCTCATGGTTCAACTCGTGGTTCTTCAATGCATCATTTGGTATATATATATATATAAATCTCTATCCTTTCGCATCTCTCTTCATTTCTGTTTTGAACTTGAAGAGATTGGAAATCCCAGAATTTCTTAGTTTGAAAACAAAGAAAGTAACATGGGCCTATTTTTTTGCCAGTTTTAGAGTGAACGTCGGTATACAAGTTGATGAAAACTTGTGGGTTTCAAACACTAAACTGGGTTTTTTTTCAATTATTGCTTGAAATGTCTGTACTTTAAGGTAGCAATATATAAGGAAGAGTTTTTAGATTTACACTCGTGTGCTGTGTGTGTAATTATTTTTTCAAAGTACGATGTTGTTCATGATTCAGCTGATCAGCTATATGACAATGTTACGAGAATCTAATGAATGTTTTAAACAGGTTTCGAAAAGATTTTTAGTATAAAGTTTAGAGAGAGAGAGATTTGATGGGTTGTTTTTTACAAGTTTTTTGAGATCTCCATGTGCAGGTATACGTTATTGCTCTTCCTCTTTGAATACAGAGCGGCGACGGTGTTGATACAGAATCAGTTCCCCGGCTCCGCCGCCGCTGCCATTACCAAGTTCGAACTCGACGGCGACGTCATCTCCCTCGACGGCAGAGACGAACTTTTGACAGAATCCGAGATCGACAACCAAGGCCGGATCCGAGTCCGGATCCGGCGGTCGACCTCGTCGGCTCCGGACTCCGGGCTGTCGTCGATCGGCATTACACCCAGAGCGTCGAATCTCTCCAACGCAGAAATCTTCTCCATAAACACGCCGGCGCGGCTCCATGACCGGCACCGCTCCACAGACATGTCTTTCGATTTGGGGGTCGGGTCCGGGTACGGGTCGTCGGACGCGTACTCTCTGCAGCCGACACCGCGGGCGTCGAACTTCAACGAGATGGAGACGACGACTGCGGGGAACACTCCGACGTGGGGGAGGTCGCCGGTGGGGGGGAGGATATTCCGGCAGGGAAGTCCGGCGCCGGCGTTTGGGAGCGTGAAGATGGTGTGGGAGTCGCCGGGGAGTAGAGAAAGAGAAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGGTACGTAGATGTTGTCGTTTTTAGAAGATGAAGGGACAAGACACTGTCGTTTTGAGAAGGACTAATTAGCAAAAACCAAAAACTGTGGGAAGTGACAGACACACTTTTCAGGTGGGCTATGTACGTTTTGTCTCCAGATAGCCTGGGAGTTGGGCCCCTCCCTTGTTAACAGAATTAACGACTAACTATAATAAGTGAAAAATAAAAATGTTAAATTACAAATTGTCCCTCTAATTTAAAAAAAGTTTCAATTTAATTTTTATGATTTTAAAATATTCAATTTAGTTTGTATAGTTTGGGCTTAGTTTCAATTTAATTTTTATGGGTCACTGAACTATAAAAAGTTTATAAATAAGTGGACTTTAAAAAATTTCTAATAGGTCTTTTAACATCAAATTTTGTATGTAATAGATCCTTATCATTAACAGTTCAATACTTGCGTCATTACATGCCAAACTTACTGAAATTAATAATTTTTAAATAATACAATCAACGTACATCGTAAGCATTAAACTAACAGAGTTATTGGAAGGGGTTATTAGACATAAAATTAAAAGTTCAAAAACCAATTAGAAACTTTTTAAAATACGTACAGAAATTTATTAGATGCAATTTTAATGACCACATTTGTAACCTAACTTTTTTTTTTTTTTTACCAAGTGTGGTGTGGTGATGTAGGGGATAAAAGGCCAAATAAATTTCCTATTGAGAGGCTGAGAACACAGTCAAAGCCATAAGTAAATATGAGAGGTTTAGTCAGAGCTTTTATTAATGAATAAAATAGTGTGTTTGTGCCATGCCATCATTTGGTAGAGAGCACGTGCATAATCATGGAGGATGGCTTTAATTGATTGCCCAATTCACAATTAGTGGTCAAGAATCCACATTATATATGATACAACAAAATCATGATGAATTGCCTACAAATTTCCTTCCTTTGATTATGTTGTCTAACCACACCTATCAATTCTTCTTTCTTTCACCTTTATATATCTTAATTACATCTTACTTATATTATAAATTTTATTTCAATCTTTCATATCATACTTCTATTTAGTCTATATGTTGTTAATGTCATTAATTGAACGTTCATACATGAAATGCTCGTTAGATAATTTTTTTGTCTTTTTTTTTAGTTCAACAATTAGTGGATGGGGGATCGAACTATCGACTTTTGAGATGGTAATAGATGTCTTATCCACTAAGCTTTTGGCAAATAAATTTTTGGTTGAATTAGTACAATGTGATAAACATTTTTAGCTAGGATACACCAATGAGTGTGTAACGTTAGCATTTTAGTAATGGAATTAGCAATAGAGAACTAAATAAAAACATAATTCAAGGTTTAAGAACGTATTGAAACTGTGTCAAACCAAGCATAACTCAATGGATAAGACATTTATTACCTTGCTTGAAGTTGAATGTTTGATCCCTTATCACCACATTTGTTGAACTAAAAAAAAAAATAGCATAGTGAAACTCGTTTTAAACTCTTTTTTTTTAAACATTAAAAAACATCAAATTAAAAGCACAAGTCTAGTGTAGTGTTTATTGATGTGTCATTGATAAAATTTTATTTTTCTTAAAATATTAGACATGTTAAAAAAATTTGCATTATATATTTGCTAACTAATTTGGTTGGTATATAAAAAAATGGTATTTCAGAAAAAGAAATTAGCTTCAGGGACAGCTCGATGACGCCCATGCCGGAGGAGGCGGCGGCGGAGGTGAAGAGCGGCCAAGAAATGCCGAGTGCTTTGGTGATGATAAGGCTTATTCTCACCGTCGTCGGTCGGAAGCTCTCCCGGAATCCAAATACATATTCCAGCGTCCTTGGCCTTCTCTGGTCCCTACTCTCATTCAAGCAAGTCCATATTTTCAACTCTATATCATTTTTATTTTTTATTTTTTTTACTTTCTGTCTCTAATATTTAATAATTTTTAGTTTGGTTAAAATTTTGGTTTAAATCTTATTTTTTATGTCAAATGTTTATACTTATTCCATTTTAGTTTTCTAACTTTTTAAGTGTTTTTGATTTTTAGTCGTTAAATTTTGCTTTATAAAAAAACTATCTTAGTTTTTAATGTTTATTTTTTTTTCATTAACTGTTTATTAAGAACCTAAATCTATTTGATAACTTGTTTGTATACGTCACTTCAAATATTAGAATATCGATTGTATCTTGATATGCAGTTGATTTGTAATAGTCTAGATATATTACATTTACTTTTAATATAAGTAGCAACTTCCACTCACGGGCATAAGTAGTCAAGTTGTTTTATTATTATTTTTTTTCCTCTAAAAATTTGAATAAAATCCCTACTCAATAAAAACAAAGAGAGCATATATGTATGAAAGTAAGTAGGAGGTTGTCCCTATGAAGTTTTAAAACTGGGGCATGATATTTAAACATTAACATGTTTTGAACGGTGTAGATGGAAGGTGGAAATGCCGAGTTTGGTGAAAAATTCGATAAAGATAATATCAGACGCTGGTTTGGGAATGGCAATGTTCAGCTTAGGATTGTTCATGGGGGTGCAGCCAAAGATAATAGGGTGTGGGGGAAAGAGGGCGACGATAGGCATGGGGATCAGGTTCATCTGCGGACCCATCGCCATGTCCGCTGCATCGCTCGGCGTTGGGCTGCGAGGTGTTAAGTTACACGCTGCCATTGTTCAGGTACTACTCCCCTGCCCTTCTTCTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCCATGACGTTTAGCCCATGCATGCATTCGCAGCTTTATATATGTAAAGTTAAAACCCTAATACTGACCTCACACTGAACTTTCTTCTGACTACCCAACCCATACCCCACTTCGAAATTCAATTTAGGGTTTTGTTTATTGCGTTTTAGTCCCCACGCTTTCGGAAGATAGTTATTTTGGTCCTTGTTATTAATTAACTTATGTCACTCTTTACCCAATCCGAATATAGTTTAGTAATTAAAACATTTCTCTACTTTTTCTAAAATGTTGTAATTTTGAACCCTTCACTTCTATATTTGTGAAGTGATATTTTAAATAAATAATTATGTGGCTCTAATATATGTGTTCATATTGGCACGTTAATACACATATTTGAAGTCAATTTATCACTTGGTTAAAGATATTAATAGAAAAATAATAGCAAGAACTCAGAGAACAAAAATTATTATTTTTTTTTTTAAGTTCAAAAACTAAATTGGTCATTTTGAAAGTTTAAAAACTAAAATGAAATAAACTTAAAGTTTAGGGACTAAAATAGATATTTTGAAAGTTTAGGGACTAAAATAAAATAATTTAAAATTTAAAATGATGAGATTTAAACCTTAATTTAAATACTGATTTTTATTTTTATTTTTTTTTGTTGGTATGTTCAGGCAGCTCTTCCACAAGGAATTGTGCCCTTCGTCTTTGCTCGGGAGTATGGATTGCATCCTGATATTCTCAGTACTGGGTTAGTATCATTTTCGTTTTTATTTTTTTTTAATTTTTATTTATGGAATATATATTATGGGGAAGAGATTCGAGTCTATGAGTGA

mRNA sequence

ATGATTACAGCGGGAGATTTCTACAAGGTGATGTGCGCGATGGTTCCGCTGTACTTCGCAATGCTGGTGGCGTACACCTCGGTGAAGTGGTGGAAGATATTCACGGCGGAGCAATGCGCCGGAATCAACCGCTTTGTGGCTGTGTTCGCTGTGCCTGTGCTTTCCTTCCATTTCATCTCTCAGAACAACCCTTACCAGATGGACACCAAGTTCATATTAGCCGACACTCTTTCCAAGCTCTTCGTGCTCCTTCTGCTCTCTCTCTGGGCCGTCTTCTTCACCGCCGGCAGCCTCGACTGGCTCATCACTCTCTTCTCTCTCGCCACTTTGCCCAACACTCTGGTCATGGGTATCCCTCTCCTCAACGCCATGTATGGCGATTCCACTCAGTCCCTCATGGTTCAACTCGTGGTTCTTCAATGCATCATTTGGTATACGTTATTGCTCTTCCTCTTTGAATACAGAGCGGCGACGGTGTTGATACAGAATCAGTTCCCCGGCTCCGCCGCCGCTGCCATTACCAAGTTCGAACTCGACGGCGACGTCATCTCCCTCGACGGCAGAGACGAACTTTTGACAGAATCCGAGATCGACAACCAAGGCCGGATCCGAGTCCGGATCCGGCGGTCGACCTCGTCGGCTCCGGACTCCGGGCTGTCGTCGATCGGCATTACACCCAGAGCGTCGAATCTCTCCAACGCAGAAATCTTCTCCATAAACACGCCGGCGCGGCTCCATGACCGGCACCGCTCCACAGACATGTCTTTCGATTTGGGGGTCGGGTCCGGGTACGGGTCGTCGGACGCGTACTCTCTGCAGCCGACACCGCGGGCGTCGAACTTCAACGAGATGGAGACGACGACTGCGGGGAACACTCCGACGTGGGGGAGGTCGCCGGTGGGGGGGAGGATATTCCGGCAGGGAAGTCCGGCGCCGGCGTTTGGGAGCGTGAAGATGGTGTGGGAGTCGCCGGGGAGTAGAGAAAGAGAAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGAAAAAGAAATTAGCTTCAGGGACAGCTCGATGACGCCCATGCCGGAGGAGGCGGCGGCGGAGGTGAAGAGCGGCCAAGAAATGCCGAGTGCTTTGGTGATGATAAGGCTTATTCTCACCGTCGTCGGTCGGAAGCTCTCCCGGAATCCAAATACATATTCCAGCGTCCTTGGCCTTCTCTGGTCCCTACTCTCATTCAAATGGAAGGTGGAAATGCCGAGTTTGGTGAAAAATTCGATAAAGATAATATCAGACGCTGGTTTGGGAATGGCAATGTTCAGCTTAGGATTGTTCATGGGGGTGCAGCCAAAGATAATAGGGTGTGGGGGAAAGAGGGCGACGATAGGCATGGGGATCAGGTTCATCTGCGGACCCATCGCCATGTCCGCTGCATCGCTCGGCGTTGGGCTGCGAGGTGTTAAGTTACACGCTGCCATTGTTCAGGCAGCTCTTCCACAAGGAATTGTGCCCTTCGTCTTTGCTCGGGAGTATGGATTGCATCCTGATATTCTCAGTACTGGGTTAGTATCATTTTCGTTTTTATTTTTTTTTAATTTTTATTTATGGAATATATATTATGGGGAAGAGATTCGAGTCTATGAGTGA

Coding sequence (CDS)

ATGATTACAGCGGGAGATTTCTACAAGGTGATGTGCGCGATGGTTCCGCTGTACTTCGCAATGCTGGTGGCGTACACCTCGGTGAAGTGGTGGAAGATATTCACGGCGGAGCAATGCGCCGGAATCAACCGCTTTGTGGCTGTGTTCGCTGTGCCTGTGCTTTCCTTCCATTTCATCTCTCAGAACAACCCTTACCAGATGGACACCAAGTTCATATTAGCCGACACTCTTTCCAAGCTCTTCGTGCTCCTTCTGCTCTCTCTCTGGGCCGTCTTCTTCACCGCCGGCAGCCTCGACTGGCTCATCACTCTCTTCTCTCTCGCCACTTTGCCCAACACTCTGGTCATGGGTATCCCTCTCCTCAACGCCATGTATGGCGATTCCACTCAGTCCCTCATGGTTCAACTCGTGGTTCTTCAATGCATCATTTGGTATACGTTATTGCTCTTCCTCTTTGAATACAGAGCGGCGACGGTGTTGATACAGAATCAGTTCCCCGGCTCCGCCGCCGCTGCCATTACCAAGTTCGAACTCGACGGCGACGTCATCTCCCTCGACGGCAGAGACGAACTTTTGACAGAATCCGAGATCGACAACCAAGGCCGGATCCGAGTCCGGATCCGGCGGTCGACCTCGTCGGCTCCGGACTCCGGGCTGTCGTCGATCGGCATTACACCCAGAGCGTCGAATCTCTCCAACGCAGAAATCTTCTCCATAAACACGCCGGCGCGGCTCCATGACCGGCACCGCTCCACAGACATGTCTTTCGATTTGGGGGTCGGGTCCGGGTACGGGTCGTCGGACGCGTACTCTCTGCAGCCGACACCGCGGGCGTCGAACTTCAACGAGATGGAGACGACGACTGCGGGGAACACTCCGACGTGGGGGAGGTCGCCGGTGGGGGGGAGGATATTCCGGCAGGGAAGTCCGGCGCCGGCGTTTGGGAGCGTGAAGATGGTGTGGGAGTCGCCGGGGAGTAGAGAAAGAGAAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGAAAAAGAAATTAGCTTCAGGGACAGCTCGATGACGCCCATGCCGGAGGAGGCGGCGGCGGAGGTGAAGAGCGGCCAAGAAATGCCGAGTGCTTTGGTGATGATAAGGCTTATTCTCACCGTCGTCGGTCGGAAGCTCTCCCGGAATCCAAATACATATTCCAGCGTCCTTGGCCTTCTCTGGTCCCTACTCTCATTCAAATGGAAGGTGGAAATGCCGAGTTTGGTGAAAAATTCGATAAAGATAATATCAGACGCTGGTTTGGGAATGGCAATGTTCAGCTTAGGATTGTTCATGGGGGTGCAGCCAAAGATAATAGGGTGTGGGGGAAAGAGGGCGACGATAGGCATGGGGATCAGGTTCATCTGCGGACCCATCGCCATGTCCGCTGCATCGCTCGGCGTTGGGCTGCGAGGTGTTAAGTTACACGCTGCCATTGTTCAGGCAGCTCTTCCACAAGGAATTGTGCCCTTCGTCTTTGCTCGGGAGTATGGATTGCATCCTGATATTCTCAGTACTGGGTTAGTATCATTTTCGTTTTTATTTTTTTTTAATTTTTATTTATGGAATATATATTATGGGGAAGAGATTCGAGTCTATGAGTGA

Protein sequence

MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFISQNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPLLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDGDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSINTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEMETTTAGNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMPEEAAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLVSFSFLFFFNFYLWNIYYGEEIRVYE
Homology
BLAST of Sgr028322 vs. NCBI nr
Match: XP_038897269.1 (auxin efflux carrier component 6 [Benincasa hispida])

HSP 1 Score: 813.9 bits (2101), Expect = 8.2e-232
Identity = 430/517 (83.17%), Postives = 461/517 (89.17%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNP++MDTKFILADT+SK+FVLLLLS+WAVFF+ G LDW+ITLFSLATLPNTLVMGIPL
Sbjct: 61  QNNPFEMDTKFILADTISKVFVLLLLSVWAVFFSGGRLDWVITLFSLATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           LNAMYGD TQ LMVQLVVLQCI+WYTLLLFLFEYRAAT+LIQNQFPGS AAAI K E+D 
Sbjct: 121 LNAMYGDFTQPLMVQLVVLQCIVWYTLLLFLFEYRAATLLIQNQFPGSTAAAIAKIEVDA 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSIN 240
           D+ISLDGRD+LLTE++ID +G+IRVRI RSTSSAP SGLSSIGITPRASNLSN EIFSIN
Sbjct: 181 DIISLDGRDKLLTETDIDTRGQIRVRICRSTSSAPSSGLSSIGITPRASNLSNVEIFSIN 240

Query: 241 TPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEMETTTAGNTPTWGRSPV 300
           TP + H  HRSTD+SFDL  GSGYGSSDAYSLQPTPRASNFNEM+   AGNTP WGRSPV
Sbjct: 241 TPTQPHGPHRSTDVSFDL--GSGYGSSDAYSLQPTPRASNFNEMD--MAGNTPMWGRSPV 300

Query: 301 GGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMPEEAA 360
           GGRIFRQGSP      VKM WESP   E   EGQGYKA LPEKEISFRDSS+T M EE  
Sbjct: 301 GGRIFRQGSPV-----VKMAWESP---ENGVEGQGYKAELPEKEISFRDSSITAMGEE-- 360

Query: 361 AEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVKNS 420
            E K  QEMP A VMI+LILTVV RKLSRNPNTYSS L LLWSL+SFKWKVEMPSLVK S
Sbjct: 361 VEAKRSQEMPKAFVMIKLILTVVTRKLSRNPNTYSSALALLWSLVSFKWKVEMPSLVKES 420

Query: 421 IKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGLRG 480
           IKIISDAGLGMAMFSLGLFMG+QPK+IGCGGKRA +GMGIRF+ GPIAMSAASL VGLRG
Sbjct: 421 IKIISDAGLGMAMFSLGLFMGLQPKMIGCGGKRAALGMGIRFLFGPIAMSAASLAVGLRG 480

Query: 481 VKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           V+LH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 VRLHTAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 503

BLAST of Sgr028322 vs. NCBI nr
Match: XP_023525387.1 (auxin efflux carrier component 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 799.7 bits (2064), Expect = 1.6e-227
Identity = 434/521 (83.30%), Postives = 456/521 (87.52%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAM+PLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITVGDFYKVMCAMLPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS---LDWLITLFSLATLPNTLVMG 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G    LDW+ITLFSLATLPNTLVMG
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLSLSLGAVFFSGGGGGRLDWVITLFSLATLPNTLVMG 120

Query: 121 IPLLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFE 180
           IPLLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA +LI NQFPG +A AITKFE
Sbjct: 121 IPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAILLIHNQFPGPSAKAITKFE 180

Query: 181 LDGDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240
           LD DVISLDGRD+LLTESEID QGRI VRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF
Sbjct: 181 LDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240

Query: 241 SINTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWG 300
           SINTP +LH   RS DM FDL  GSGYGSSDAYSLQPTPR SNFN+ME TTTAGNTPTWG
Sbjct: 241 SINTPVQLHGPLRSNDMLFDL--GSGYGSSDAYSLQPTPRGSNFNDMEMTTTAGNTPTWG 300

Query: 301 RSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMP 360
           RSPV G             +VKMVWESP      GEGQGYKAVLP KE+SFRDS+MT M 
Sbjct: 301 RSPVAGS-----------SAVKMVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSTMTAMG 360

Query: 361 EEAAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSL 420
           EE   E K  QE+P+A VMIRLILTVVGRKLSRNPNTYSSVLGLLWSL SFKWKV MPS+
Sbjct: 361 EE--VEAKRSQELPNAFVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLASFKWKVAMPSV 420

Query: 421 VKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGV 480
           V  SIKIISDAGLGMAMFSLGLFM +QP+IIGCGGKRA IGMGIRFICGPIAMSAASLGV
Sbjct: 421 VVYSIKIISDAGLGMAMFSLGLFMALQPRIIGCGGKRACIGMGIRFICGPIAMSAASLGV 480

Query: 481 GLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           GLRGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 GLRGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 505

BLAST of Sgr028322 vs. NCBI nr
Match: XP_022981447.1 (auxin efflux carrier component 6 [Cucurbita maxima])

HSP 1 Score: 799.3 bits (2063), Expect = 2.1e-227
Identity = 435/519 (83.82%), Postives = 456/519 (87.86%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS-LDWLITLFSLATLPNTLVMGIP 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G  LDW+ITLFSLATLPNTLVMGIP
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGGGRLDWVITLFSLATLPNTLVMGIP 120

Query: 121 LLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELD 180
           LLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA  LI NQFPG +A AITKFELD
Sbjct: 121 LLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKFELD 180

Query: 181 GDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI 240
            DVISLDGRD+LLTESEID QGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI
Sbjct: 181 ADVISLDGRDKLLTESEIDIQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI 240

Query: 241 NTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWGRS 300
           NTP +LH   RS DMSFDL  GSGYGSSDAYSLQPTPRASNFN+ME TTTAGNTPTWGRS
Sbjct: 241 NTPVQLHGPLRSNDMSFDL--GSGYGSSDAYSLQPTPRASNFNDMELTTTAGNTPTWGRS 300

Query: 301 PVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMPEE 360
           PV G             +VKMVWESP      GEGQGYKAVLP KE+SFRDS++T M EE
Sbjct: 301 PVAGS-----------SAVKMVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSTITAMAEE 360

Query: 361 AAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVK 420
              E K  QE+P+A VMIRLILTVVGRKLSRNPNTYSSVLGLL SL SFKWKV MPS+V 
Sbjct: 361 --VEAKRSQELPNAFVMIRLILTVVGRKLSRNPNTYSSVLGLLLSLASFKWKVVMPSVVV 420

Query: 421 NSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGL 480
            SIKIISDAGLGMAMFSLGLFM +QP+II CGGKRA IGMGIRFICGPIAMSAASLGVGL
Sbjct: 421 YSIKIISDAGLGMAMFSLGLFMALQPRIIACGGKRACIGMGIRFICGPIAMSAASLGVGL 480

Query: 481 RGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           RGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 RGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 503

BLAST of Sgr028322 vs. NCBI nr
Match: KAG6607665.1 (Auxin efflux carrier component 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-225
Identity = 431/521 (82.73%), Postives = 453/521 (86.95%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS---LDWLITLFSLATLPNTLVMG 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G    LDW+ITLFSLATLPNTLVMG
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGGGGGRLDWVITLFSLATLPNTLVMG 120

Query: 121 IPLLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFE 180
           IPLLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA  LI NQFPG +A AITK E
Sbjct: 121 IPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKIE 180

Query: 181 LDGDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240
           LD DVISLDGRD+LLTESEID QGRI VRIRRSTSSAPDSG SSIGITPRASNLSNAEIF
Sbjct: 181 LDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGFSSIGITPRASNLSNAEIF 240

Query: 241 SINTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWG 300
           SINTP +LH   RS DMSFDL  GSGYGSSDAYSLQPTPR SNFN+ME TTTAGNTPTWG
Sbjct: 241 SINTPVQLHGPLRSNDMSFDL--GSGYGSSDAYSLQPTPRGSNFNDMEMTTTAGNTPTWG 300

Query: 301 RSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMP 360
           RSPV G             +VK+VWESP      GEGQGYKAVLP KE+SFRDS++T M 
Sbjct: 301 RSPVAGS-----------SAVKIVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSTITAMG 360

Query: 361 EEAAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSL 420
           EE   E K  QE+P+A VMIRLILTVVGRKLSRNPNTYSSVLGLLWSL SFKWKV MPS+
Sbjct: 361 EE--VEAKRSQELPNAFVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLASFKWKVAMPSV 420

Query: 421 VKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGV 480
           V  SIKIISDAGLGMAMFSLGLFM +QP+II CGGKRA IGMGIRFICGPIAMSAASLGV
Sbjct: 421 VVYSIKIISDAGLGMAMFSLGLFMALQPRIIACGGKRACIGMGIRFICGPIAMSAASLGV 480

Query: 481 GLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           GLRGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 GLRGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 505

BLAST of Sgr028322 vs. NCBI nr
Match: XP_022926200.1 (auxin efflux carrier component 6 [Cucurbita moschata])

HSP 1 Score: 790.0 bits (2039), Expect = 1.3e-224
Identity = 429/521 (82.34%), Postives = 452/521 (86.76%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITVGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS---LDWLITLFSLATLPNTLVMG 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G    LDW+ITLFSLATLPNTLVMG
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGGGGGRLDWVITLFSLATLPNTLVMG 120

Query: 121 IPLLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFE 180
           IPLLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA  LI NQFPG +A AITK E
Sbjct: 121 IPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKIE 180

Query: 181 LDGDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240
           LD DVISLDGRD+LLTESEID QGRI VRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF
Sbjct: 181 LDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240

Query: 241 SINTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWG 300
           SINTP +LH   RS DMSFDL  GSGYGSSDAYS+QPTPR SNFN+ME TTTAGNT TWG
Sbjct: 241 SINTPVQLHGPLRSNDMSFDL--GSGYGSSDAYSVQPTPRGSNFNDMEMTTTAGNTATWG 300

Query: 301 RSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMP 360
           RSPV G             +VKMVWESP      GEGQGYKAVLP KE+SFRDS++  M 
Sbjct: 301 RSPVAGS-----------SAVKMVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSAIAAMA 360

Query: 361 EEAAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSL 420
           EE   E K  QE+P+A VM+RLILTVVGRKLSRNPNTYSSVLGLLWSL SFKWKV MPS+
Sbjct: 361 EE--VEAKRSQELPNAFVMMRLILTVVGRKLSRNPNTYSSVLGLLWSLASFKWKVAMPSV 420

Query: 421 VKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGV 480
           V  SIKIISDAGLGMAMFSLGLFM +QP+II CGGKRA IGMGIRFICGPIAMSAASLGV
Sbjct: 421 VVYSIKIISDAGLGMAMFSLGLFMALQPRIIACGGKRACIGMGIRFICGPIAMSAASLGV 480

Query: 481 GLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           GLRGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 GLRGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 505

BLAST of Sgr028322 vs. ExPASy Swiss-Prot
Match: Q9SQH6 (Auxin efflux carrier component 6 OS=Arabidopsis thaliana OX=3702 GN=PIN6 PE=2 SV=2)

HSP 1 Score: 640.6 bits (1651), Expect = 1.6e-182
Identity = 372/568 (65.49%), Postives = 423/568 (74.47%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  +FY VMCAM PLYFAM VAY SVKW KIFT  QC+GINRFV+VFAVPVLSFHFIS
Sbjct: 1   MITGNEFYTVMCAMAPLYFAMFVAYGSVKWCKIFTPAQCSGINRFVSVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNPY+MDT FILADTLSK+FV +LLSLWAVFF AG LDWLITLFS+ATLPNTLVMGIPL
Sbjct: 61  QNNPYKMDTMFILADTLSKIFVFVLLSLWAVFFKAGGLDWLITLFSIATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYGD TQ+LMVQLVVLQCIIWYTLLLFLFE RAA +LI+ +FPG AA +I K ++D 
Sbjct: 121 LQAMYGDYTQTLMVQLVVLQCIIWYTLLLFLFELRAARLLIRAEFPGQAAGSIAKIQVDD 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGL-SSIGITPRASNLSNAEIFSI 240
           DVISLDG D L TE+E D  GRIR+RIRRS SS PDS + SS+ +TPRASNLSNAEIFS+
Sbjct: 181 DVISLDGMDPLRTETETDVNGRIRLRIRRSVSSVPDSVMSSSLCLTPRASNLSNAEIFSV 240

Query: 241 NTP-----------ARLHDRHRSTDMSF---DL--------GVG------SGYGSSDAYS 300
           NTP             L   + S ++ F   DL        G+G      SGY SSDAYS
Sbjct: 241 NTPNNRFFHGGGGSGTLQFYNGSNEIMFCNGDLGGFGFTRPGLGASPRRLSGYASSDAYS 300

Query: 301 LQPTPRASNFNEMETTTAGNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREG 360
           LQPTPRASNFNE++    G TP W +SP  GRI+RQ SP       KM+WES G R    
Sbjct: 301 LQPTPRASNFNELDVNGNG-TPVWMKSPAAGRIYRQSSP-------KMMWES-GQRHAAK 360

Query: 361 EGQGYKAVLPEKEISFRDSSMTPMPEEAA---AEVKSG--------------QEMPSALV 420
           +  G    +PEKEISFRD+        AA   A ++ G              QEMPSA+V
Sbjct: 361 DING---SVPEKEISFRDALKAAPQATAAGGGASMEEGAAGKDTTPVAAIGKQEMPSAIV 420

Query: 421 MIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMF 480
           M+RLILTVVGRKLSRNPNTYSS+LGL+WSL+SFKW + MP++V  SIKIISDAGLGMAMF
Sbjct: 421 MMRLILTVVGRKLSRNPNTYSSLLGLVWSLISFKWNIPMPNIVDFSIKIISDAGLGMAMF 480

Query: 481 SLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGLRGVKLHAAIVQAALPQ 523
           SLGLFM +QPK+I CG K+AT+GM IRFI GP+ M+ ASL VGLRG +LHAAIVQAALPQ
Sbjct: 481 SLGLFMALQPKMIPCGAKKATMGMLIRFISGPLFMAGASLLVGLRGSRLHAAIVQAALPQ 540

BLAST of Sgr028322 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.7e-134
Identity = 291/577 (50.43%), Postives = 358/577 (62.05%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  DFY VM AMVPLY AM++AY SVKWW+IFT +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +FI ADTL KL VL LL+LW+     GSL+W ITLFSL+TLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L  MYG+ + SLMVQ+VVLQCIIWYTL+LF+FEYR A +LI  QFP   A AI    +D 
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFP-DTAGAIASIVVDA 180

Query: 181 DVISLDGRDELL-TESEIDNQGRIRVRIRRSTSSAPD------SGLSSIGITPRASNLSN 240
           DV+SLDGR +++ TE+E+   G+I V +RRS +S  D       G SS   TPR SNL+N
Sbjct: 181 DVVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFSS--TTPRPSNLTN 240

Query: 241 AEIFSINT---PARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQ--PTPRASNFNEMETTT 300
           AEI+S+ +   P         TD    +G  S + + DA+ ++   TPR SN+ E     
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFAAGDAFGVRTGATPRPSNYEEDAAAP 300

Query: 301 AGNTPTWGRSPVGGRIFRQGSPAP---AFGSVK---------MVWESPGSRERE--GEGQ 360
                 +G+ P          P P   A G  K          VW S  S   +  G G 
Sbjct: 301 NKAGSKYGQYPAPNPAM-AAPPKPKKAANGQAKGEDGKDLHMFVWSSSASPVSDVFGNGA 360

Query: 361 GYKAVLPEKEI------------------SFRDSSMTPMPEEA-----AAEVKSGQE--- 420
            Y      KE+                  SF +  +     EA      A   SG+    
Sbjct: 361 EYNDAAAVKEVRMAVASPRKADGVERDDFSFGNRGVAERDAEAGDEKSVAAAVSGEHGKP 420

Query: 421 --------MPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVKNS 480
                   MP   VM RLIL +V RKL RNPNTYSS++GL+WSL+ F+W  EMP+++  S
Sbjct: 421 GLTPAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVCFRWNFEMPAIILKS 480

Query: 481 IKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGLRG 518
           I I+SDAGLGMAMFSLGLFM +QP+II CG K AT  M +RF+ GP  M+AAS+ VGLRG
Sbjct: 481 ISILSDAGLGMAMFSLGLFMALQPRIIACGNKVATFAMAVRFLTGPAVMAAASIAVGLRG 540

BLAST of Sgr028322 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.5e-132
Identity = 287/582 (49.31%), Postives = 357/582 (61.34%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MITA DFY VM AMVPLY AM++AY SVKWW+IFT +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +FI ADTL KL VL +L+ W+     GSL+W ITLFSL+TLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L  MYG+ + SLMVQ+VVLQCIIWYTL+LF+FEYR A +LI  QFP   AA I    +D 
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFP-DTAANIASIVVDP 180

Query: 181 DVISLDG-RDELLTESEIDNQGRIRVRIRRSTSSAPD------SGLSSIGITPRASNLSN 240
           DV+SLDG RD + TE+E+   GRI V +RRS +S  D       G SS   TPR SNL+N
Sbjct: 181 DVVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIYSRRSMGFSS--TTPRPSNLTN 240

Query: 241 AEIFSINT---PARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQ--PTPRASNFNEMETTT 300
           AEI+S+ +   P         TD    +G  S +G++DA+ ++   TPR SN+ +  +  
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADAFGVRTGATPRPSNYEDDASKP 300

Query: 301 AGNTPTWGRSPVGGRIFRQGSPAPAFGSV-------------------KMVWES------ 360
               P    +P+ G      +P PA  S                      VW S      
Sbjct: 301 KYPLPASNAAPMAG---HYPAPNPAVSSAPKGAKKAATNGQAKGEDLHMFVWSSSASPVS 360

Query: 361 ----------------------PGSREREGEGQGYKAVLPEKEISFRDSSMTPMPEEAAA 420
                                  G+++RE   +        + +  RD+        AAA
Sbjct: 361 DVFGGGAPDYNDAAAVKSPRKMDGAKDREDYVERDDFSFGNRGVMDRDAEAGDEKAAAAA 420

Query: 421 ------EVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPS 480
                  + +   MP   VM RLIL +V RKL RNPNTYSS++GL+WSL+ F+W  EMP+
Sbjct: 421 GADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVCFRWNFEMPA 480

Query: 481 LVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLG 518
           +V  SI I+SDAGLGMAMFSLGLFM +QP II CG K AT  M +RF+ GP  M+AAS  
Sbjct: 481 IVLKSISILSDAGLGMAMFSLGLFMALQPHIIACGNKVATYAMAVRFLAGPAVMAAASFA 540

BLAST of Sgr028322 vs. ExPASy Swiss-Prot
Match: Q940Y5 (Auxin efflux carrier component 7 OS=Arabidopsis thaliana OX=3702 GN=PIN7 PE=1 SV=2)

HSP 1 Score: 466.1 bits (1198), Expect = 5.5e-130
Identity = 289/604 (47.85%), Postives = 368/604 (60.93%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +FI ADTL KL +L LL +WA F  +GSL+W IT+FSL+TLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYG+ + SLMVQ+VVLQCIIWYTLLLFLFEYR A +LI  QFP    A+I  F+++ 
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSFKVES 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPD-SGLSSIGITPRASNLSNAEIFSI 240
           DV+SLDG D L T+++I + G++ V +R+S +S     G     +TPR SNL+ AEI+S+
Sbjct: 181 DVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSL 240

Query: 241 NTPARLHDRHRS---TDMSFDLGVGSGYGSSDAYSLQ----PTPRASNFNEMETTTAGNT 300
           NT  R  + + S   + M F  G  S +G +D YS+Q    PTPR SNF   E+    ++
Sbjct: 241 NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFE--ESCAMASS 300

Query: 301 PTWGRSPVGGRIFRQGSPAPAFGSVK---------------------------MVWESPG 360
           P +G  P GG      +P P F +                              VW S G
Sbjct: 301 PRFGYYP-GGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNG 360

Query: 361 S--REREG--------EGQGYKAVLPEKEISFRDSSMT--------PM--------PEEA 420
           S   +R G        E  G       KEI    S  T        PM          E 
Sbjct: 361 SPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNGENKAGPMNGDYGGEEESER 420

Query: 421 AAEVKSG--------------------------QEMPSALVMIRLILTVVGRKLSRNPNT 480
             EV +G                          + MP A VM RLIL +V RKL RNPNT
Sbjct: 421 VKEVPNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWRKLIRNPNT 480

Query: 481 YSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKR 518
           YSS++GL+W+L++F+W V MP +++ SI I+SDAGLGMAMFSLGLFM +QPK+I CG   
Sbjct: 481 YSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNST 540

BLAST of Sgr028322 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.4e-128
Identity = 291/608 (47.86%), Postives = 360/608 (59.21%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MITA DFY VM AMVPLY AM++AY SVKWWKIFT +QC+GINRFVA+FAVP+LSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +F+ AD+L K+ VL LL LW      GSLDW ITLFSL+TLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L  MYG+ +  LMVQ+VVLQCIIWYTL+LFLFEYR A +LI  QFP   A +I    +D 
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFP-DTAGSIVSIHVDS 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPD------SGLSSIGITPRASNLSNA 240
           D++SLDGR  L TE+EI   G++ V +RRS +S  D       GLS+   TPR SNL+NA
Sbjct: 181 DIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA---TPRPSNLTNA 240

Query: 241 EIFSINT---PARLHDRHRSTD----MSFDLGVGSGYGSSDAY--SLQPTPRASNFNE-- 300
           EI+S+ +   P         TD    M+   G  S +G  +A   S  PTPR SN+ E  
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDG 300

Query: 301 --METTTAGNTPTWGR------------------------SP---VGGRIFRQGSPAPAF 360
              + T AG     GR                        SP    GG    +G+ AP  
Sbjct: 301 GPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGN-APVV 360

Query: 361 GSVK----------MVWESPGSREREGEGQG------------------YKAVLPE---- 420
           G  +           VW S  S   +  G G                   K  +P+    
Sbjct: 361 GGKRQDGNGRDLHMFVWSSSASPVSDVFGGGGGNHHADYSTATNDHQKDVKISVPQGNSN 420

Query: 421 -------KEISF--RDSSMTPMPEEAAAEVKS----GQEMPSALVMIRLILTVVGRKLSR 480
                  +E SF  +D     +  +    + +     + MP   VM RLIL +V RKL R
Sbjct: 421 DNQYVEREEFSFGNKDDDSKVLATDGGNNISNKTTQAKVMPPTSVMTRLILIMVWRKLIR 480

Query: 481 NPNTYSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGC 518
           NPN+YSS+ G+ WSL+SFKW +EMP+L+  SI I+SDAGLGMAMFSLGLFM + P+II C
Sbjct: 481 NPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIAC 540

BLAST of Sgr028322 vs. ExPASy TrEMBL
Match: A0A6J1ITZ9 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111480565 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 1.0e-227
Identity = 435/519 (83.82%), Postives = 456/519 (87.86%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS-LDWLITLFSLATLPNTLVMGIP 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G  LDW+ITLFSLATLPNTLVMGIP
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGGGRLDWVITLFSLATLPNTLVMGIP 120

Query: 121 LLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELD 180
           LLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA  LI NQFPG +A AITKFELD
Sbjct: 121 LLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKFELD 180

Query: 181 GDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI 240
            DVISLDGRD+LLTESEID QGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI
Sbjct: 181 ADVISLDGRDKLLTESEIDIQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSI 240

Query: 241 NTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWGRS 300
           NTP +LH   RS DMSFDL  GSGYGSSDAYSLQPTPRASNFN+ME TTTAGNTPTWGRS
Sbjct: 241 NTPVQLHGPLRSNDMSFDL--GSGYGSSDAYSLQPTPRASNFNDMELTTTAGNTPTWGRS 300

Query: 301 PVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMPEE 360
           PV G             +VKMVWESP      GEGQGYKAVLP KE+SFRDS++T M EE
Sbjct: 301 PVAGS-----------SAVKMVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSTITAMAEE 360

Query: 361 AAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVK 420
              E K  QE+P+A VMIRLILTVVGRKLSRNPNTYSSVLGLL SL SFKWKV MPS+V 
Sbjct: 361 --VEAKRSQELPNAFVMIRLILTVVGRKLSRNPNTYSSVLGLLLSLASFKWKVVMPSVVV 420

Query: 421 NSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGL 480
            SIKIISDAGLGMAMFSLGLFM +QP+II CGGKRA IGMGIRFICGPIAMSAASLGVGL
Sbjct: 421 YSIKIISDAGLGMAMFSLGLFMALQPRIIACGGKRACIGMGIRFICGPIAMSAASLGVGL 480

Query: 481 RGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           RGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 RGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 503

BLAST of Sgr028322 vs. ExPASy TrEMBL
Match: A0A6J1EKF9 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111433384 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 6.1e-225
Identity = 429/521 (82.34%), Postives = 452/521 (86.76%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT GDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS
Sbjct: 1   MITVGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGS---LDWLITLFSLATLPNTLVMG 120
           QNNP++MDTKFILADT+SK FVLL LSL AVFF+ G    LDW+ITLFSLATLPNTLVMG
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGGGGGRLDWVITLFSLATLPNTLVMG 120

Query: 121 IPLLNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFE 180
           IPLLNAMYGD TQ LMVQL+VLQCIIWYTLLLFLFEYRAA  LI NQFPG +A AITK E
Sbjct: 121 IPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKIE 180

Query: 181 LDGDVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240
           LD DVISLDGRD+LLTESEID QGRI VRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF
Sbjct: 181 LDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIF 240

Query: 241 SINTPARLHDRHRSTDMSFDLGVGSGYGSSDAYSLQPTPRASNFNEME-TTTAGNTPTWG 300
           SINTP +LH   RS DMSFDL  GSGYGSSDAYS+QPTPR SNFN+ME TTTAGNT TWG
Sbjct: 241 SINTPVQLHGPLRSNDMSFDL--GSGYGSSDAYSVQPTPRGSNFNDMEMTTTAGNTATWG 300

Query: 301 RSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREGEGQGYKAVLPEKEISFRDSSMTPMP 360
           RSPV G             +VKMVWESP      GEGQGYKAVLP KE+SFRDS++  M 
Sbjct: 301 RSPVAGS-----------SAVKMVWESP-ENGGGGEGQGYKAVLPVKEMSFRDSAIAAMA 360

Query: 361 EEAAAEVKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSL 420
           EE   E K  QE+P+A VM+RLILTVVGRKLSRNPNTYSSVLGLLWSL SFKWKV MPS+
Sbjct: 361 EE--VEAKRSQELPNAFVMMRLILTVVGRKLSRNPNTYSSVLGLLWSLASFKWKVAMPSV 420

Query: 421 VKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGV 480
           V  SIKIISDAGLGMAMFSLGLFM +QP+II CGGKRA IGMGIRFICGPIAMSAASLGV
Sbjct: 421 VVYSIKIISDAGLGMAMFSLGLFMALQPRIIACGGKRACIGMGIRFICGPIAMSAASLGV 480

Query: 481 GLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           GLRGVKLH AIVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 GLRGVKLHVAIVQAALPQGIVPFVFAREYGLHPDILSTGVI 505

BLAST of Sgr028322 vs. ExPASy TrEMBL
Match: F6H096 (Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=VIT_18s0001g15420 PE=3 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 9.8e-215
Identity = 413/534 (77.34%), Postives = 456/534 (85.39%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MI+A DFYKVMCAMVPLYFAMLVAY SVKW KIF+ EQC+GINRFVAVFAVPVLSFHFIS
Sbjct: 1   MISADDFYKVMCAMVPLYFAMLVAYGSVKWCKIFSPEQCSGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNPY+MDTKFI+ADTLSKL VL+LLS+WA+ F  G LDWLITLFSLATLPNTLVMGIPL
Sbjct: 61  QNNPYEMDTKFIVADTLSKLLVLVLLSVWAILF-KGGLDWLITLFSLATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           LNAMYGD TQSLMVQLVVLQCIIWYTLLLFLFEYRAAT+LI+NQFPGS AA+I+KFE+DG
Sbjct: 121 LNAMYGDFTQSLMVQLVVLQCIIWYTLLLFLFEYRAATLLIKNQFPGSTAASISKFEIDG 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGL-SSIGITPRASNLSNAEIFSI 240
           DVISLDGRD + TESEID  GRIRVRIRRSTSSAPDS L SS+GITPRASNLS AEIFS+
Sbjct: 181 DVISLDGRDPVRTESEIDGNGRIRVRIRRSTSSAPDSALSSSMGITPRASNLSGAEIFSV 240

Query: 241 NTPARLHDRHR-STDMSF---DLGVG--------SGYGSSDAYSLQPTPRASNFNEMETT 300
           NTPA LH+ H  + D++F   DLG G        SGY SSDAYSLQPTPRASNFNE++TT
Sbjct: 241 NTPAPLHEYHNGNADIAFGNGDLGFGYRSASPRLSGYASSDAYSLQPTPRASNFNELDTT 300

Query: 301 --TAGNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGS-REREGEGQGYKAVLPEKE 360
             T  NTP W RSPV G+++RQ S  PAF  V+MVWESPG      GE QG K V+ E+E
Sbjct: 301 TITTTNTPFWVRSPVAGKVYRQQS--PAFSGVRMVWESPGKCNGGGGERQGCKDVVGERE 360

Query: 361 ISFRDSSMTPMPEEAAAE-VKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWS 420
           ISFRDSS  P+ EE   +   +GQ+MP   VM+RLILT+VGRKLSRNPNTYSSVLGLLWS
Sbjct: 361 ISFRDSSKFPVAEEEDPKATATGQDMPRTHVMLRLILTMVGRKLSRNPNTYSSVLGLLWS 420

Query: 421 LLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFI 480
           L+SFKW V MPSLVK SIKIISDAGLGMAMFSLGLFM +QP+II CG KRAT+GMGIRFI
Sbjct: 421 LISFKWNVGMPSLVKYSIKIISDAGLGMAMFSLGLFMALQPRIIACGTKRATMGMGIRFI 480

Query: 481 CGPIAMSAASLGVGLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           CGPI MSAAS+ VGLRGV+LHAAIVQA+LPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 CGPILMSAASIAVGLRGVRLHAAIVQASLPQGIVPFVFAREYGLHPDILSTGVI 531

BLAST of Sgr028322 vs. ExPASy TrEMBL
Match: A0A5J5BUQ1 (Auxin efflux carrier component OS=Nyssa sinensis OX=561372 GN=F0562_002743 PE=3 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 2.4e-213
Identity = 407/531 (76.65%), Postives = 451/531 (84.93%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MI+A DFYKVMCA+VPLYFAML+AY SVKWWKIFT EQC+GINRFVAVFAVPVLSFHFIS
Sbjct: 1   MISANDFYKVMCALVPLYFAMLIAYASVKWWKIFTPEQCSGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNPYQMDTKFILADTLSKL VL+LLSLWA+F   G LDWLITLFS+ATLPNTLVMGIPL
Sbjct: 61  QNNPYQMDTKFILADTLSKLIVLVLLSLWAIF--KGGLDWLITLFSVATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYGD TQSLMVQLVVLQCIIWYTLLLFLFEYRAA +LIQNQFPGS AA ITK E+DG
Sbjct: 121 LKAMYGDFTQSLMVQLVVLQCIIWYTLLLFLFEYRAAAILIQNQFPGSTAACITKIEIDG 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGL-SSIGITPRASNLSNAEIFSI 240
           DVISLDGRD L TES+ID  GRIRVRIRRSTSSAPDS L SSIGITPRASNLS AEI+S+
Sbjct: 181 DVISLDGRDPLCTESDIDGNGRIRVRIRRSTSSAPDSALSSSIGITPRASNLSGAEIYSV 240

Query: 241 NTPARLHD-RHRSTDMSF-DLGVG-------SGYGSSDAYSLQPTPRASNFNEMETTTA- 300
           NTPA  H+  H STD++F DL  G       SGY SSDAYSLQPTPRASNFNE++TTTA 
Sbjct: 241 NTPAPAHEFHHASTDIAFGDLAFGYRSSPRLSGYASSDAYSLQPTPRASNFNELDTTTAT 300

Query: 301 -GNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGS-REREGEGQGYKAVLPEKEISF 360
            GNTP W RSP G ++FRQ S  PAF  +KMVWESPG  +   GE QG K V+ +K++SF
Sbjct: 301 TGNTPIWIRSPAGAKVFRQSS--PAFSGMKMVWESPGKCQGGGGERQGCKDVVGDKDLSF 360

Query: 361 RDSSMTPMPEEAAAE-VKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWSLLS 420
           RD +  PM EE  ++  +  Q+MP+A+VM+RLIL +VGRKLSRNPNTYSSVLGLLWSL+S
Sbjct: 361 RDCAKFPMAEETDSKGAEVPQKMPNAIVMLRLILIMVGRKLSRNPNTYSSVLGLLWSLIS 420

Query: 421 FKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFICGP 480
           FKW V +PSLVK SIKIISDAGLGMAMFSLGLFM +QP+II CG K ATIGM IRF+ GP
Sbjct: 421 FKWNVGVPSLVKYSIKIISDAGLGMAMFSLGLFMALQPRIIACGTKMATIGMVIRFVGGP 480

Query: 481 IAMSAASLGVGLRGVKLHAAIVQAALPQGIVPFVFAREYGLHPDILSTGLV 518
           + MSAAS+ VGLRGVKLH +IVQAALPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 VLMSAASIAVGLRGVKLHTSIVQAALPQGIVPFVFAREYGLHPDILSTGVI 527

BLAST of Sgr028322 vs. ExPASy TrEMBL
Match: A0A438HKK3 (Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN6_0 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 2.0e-212
Identity = 412/539 (76.44%), Postives = 455/539 (84.42%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MI+A DFYKVMCAMVPLYFAMLVAY SVKW KIF+ EQC+GINRFVAVFAVPVLSFHFIS
Sbjct: 1   MISADDFYKVMCAMVPLYFAMLVAYGSVKWCKIFSPEQCSGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNPY+MDTKFI+ADTLSKL VL+LLS+WA+ F  G LDWLITLFSLATLPNTLVMGIPL
Sbjct: 61  QNNPYEMDTKFIVADTLSKLLVLVLLSVWAILF-KGGLDWLITLFSLATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           LNAMYGD TQSLMVQLVVLQCIIWYTLLLFLFEYRAAT+LI+NQFPGS AA+I+KFE+DG
Sbjct: 121 LNAMYGDFTQSLMVQLVVLQCIIWYTLLLFLFEYRAATLLIKNQFPGSTAASISKFEIDG 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGL-SSIGITPRASNLSNAEIFSI 240
           DVISLDGRD + TESEID  GRIRVRIRRSTSSAPDS L SS+GITPRASNLS AEIFS+
Sbjct: 181 DVISLDGRDPVRTESEIDGNGRIRVRIRRSTSSAPDSALSSSMGITPRASNLSGAEIFSV 240

Query: 241 NTPARLHDRHR-STDMSF---DLGVG--------SGYGSSDAYSLQPTPRASNFNEMETT 300
           NTPA LH+ H  + D++F   DLG G        SGY SSDAYSLQPTPRASNFNE++TT
Sbjct: 241 NTPAPLHEYHNGNADIAFGNGDLGFGYRSASPRLSGYASSDAYSLQPTPRASNFNELDTT 300

Query: 301 --TAGNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGS-REREGEGQGYKAVLPEKE 360
             T  NTP W RSPV G+++RQ S  PAF  V+MVWESPG      GE QG K V+ E+E
Sbjct: 301 TITTTNTPFWVRSPVAGKVYRQQS--PAFSGVRMVWESPGKCNGGGGERQGCKDVVGERE 360

Query: 361 ISFRDSSMTPMPEEAAAE-VKSGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVLGLLWS 420
           ISFRDSS  P+ EE   +   +GQ+MP   VM+RLILT+VGRKLSRNPNTYSSV+GLLWS
Sbjct: 361 ISFRDSSKFPVAEEEDPKATATGQDMPRTHVMLRLILTMVGRKLSRNPNTYSSVIGLLWS 420

Query: 421 LLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGMGIRFI 480
           L+SFKW V MPSLVK SIKIISDAGLGMAMFSLGLFM +QP+II CG KRAT+GMGIRFI
Sbjct: 421 LISFKWNVGMPSLVKYSIKIISDAGLGMAMFSLGLFMALQPQIIACGTKRATMGMGIRFI 480

Query: 481 CGPIAMSAASLGVGLRGVKLHAAIVQAA-----LPQGIVPFVFAREYGLHPDILSTGLV 518
           CGPI MSAAS+ VGLRGV+LHAAIVQA      LPQGIVPFVFAREYGLHPDILSTG++
Sbjct: 481 CGPILMSAASIAVGLRGVRLHAAIVQACCIFIPLPQGIVPFVFAREYGLHPDILSTGVI 536

BLAST of Sgr028322 vs. TAIR 10
Match: AT1G77110.1 (Auxin efflux carrier family protein )

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-183
Identity = 372/568 (65.49%), Postives = 423/568 (74.47%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  +FY VMCAM PLYFAM VAY SVKW KIFT  QC+GINRFV+VFAVPVLSFHFIS
Sbjct: 1   MITGNEFYTVMCAMAPLYFAMFVAYGSVKWCKIFTPAQCSGINRFVSVFAVPVLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
           QNNPY+MDT FILADTLSK+FV +LLSLWAVFF AG LDWLITLFS+ATLPNTLVMGIPL
Sbjct: 61  QNNPYKMDTMFILADTLSKIFVFVLLSLWAVFFKAGGLDWLITLFSIATLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYGD TQ+LMVQLVVLQCIIWYTLLLFLFE RAA +LI+ +FPG AA +I K ++D 
Sbjct: 121 LQAMYGDYTQTLMVQLVVLQCIIWYTLLLFLFELRAARLLIRAEFPGQAAGSIAKIQVDD 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGL-SSIGITPRASNLSNAEIFSI 240
           DVISLDG D L TE+E D  GRIR+RIRRS SS PDS + SS+ +TPRASNLSNAEIFS+
Sbjct: 181 DVISLDGMDPLRTETETDVNGRIRLRIRRSVSSVPDSVMSSSLCLTPRASNLSNAEIFSV 240

Query: 241 NTP-----------ARLHDRHRSTDMSF---DL--------GVG------SGYGSSDAYS 300
           NTP             L   + S ++ F   DL        G+G      SGY SSDAYS
Sbjct: 241 NTPNNRFFHGGGGSGTLQFYNGSNEIMFCNGDLGGFGFTRPGLGASPRRLSGYASSDAYS 300

Query: 301 LQPTPRASNFNEMETTTAGNTPTWGRSPVGGRIFRQGSPAPAFGSVKMVWESPGSREREG 360
           LQPTPRASNFNE++    G TP W +SP  GRI+RQ SP       KM+WES G R    
Sbjct: 301 LQPTPRASNFNELDVNGNG-TPVWMKSPAAGRIYRQSSP-------KMMWES-GQRHAAK 360

Query: 361 EGQGYKAVLPEKEISFRDSSMTPMPEEAA---AEVKSG--------------QEMPSALV 420
           +  G    +PEKEISFRD+        AA   A ++ G              QEMPSA+V
Sbjct: 361 DING---SVPEKEISFRDALKAAPQATAAGGGASMEEGAAGKDTTPVAAIGKQEMPSAIV 420

Query: 421 MIRLILTVVGRKLSRNPNTYSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMF 480
           M+RLILTVVGRKLSRNPNTYSS+LGL+WSL+SFKW + MP++V  SIKIISDAGLGMAMF
Sbjct: 421 MMRLILTVVGRKLSRNPNTYSSLLGLVWSLISFKWNIPMPNIVDFSIKIISDAGLGMAMF 480

Query: 481 SLGLFMGVQPKIIGCGGKRATIGMGIRFICGPIAMSAASLGVGLRGVKLHAAIVQAALPQ 523
           SLGLFM +QPK+I CG K+AT+GM IRFI GP+ M+ ASL VGLRG +LHAAIVQAALPQ
Sbjct: 481 SLGLFMALQPKMIPCGAKKATMGMLIRFISGPLFMAGASLLVGLRGSRLHAAIVQAALPQ 540

BLAST of Sgr028322 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 467.6 bits (1202), Expect = 1.3e-131
Identity = 285/600 (47.50%), Postives = 366/600 (61.00%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +FI ADTL KL +L LL +WA F  +GSL+W IT+FSL+TLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYG+ + SLMVQ+VVLQCIIWYTLLLFLFEYR A +LI  QFP    A+I  F+++ 
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSFKVES 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPD-SGLSSIGITPRASNLSNAEIFSI 240
           DV+SLDG D L T+++I + G++ V +R+S +S     G     +TPR SNL+ AEI+S+
Sbjct: 181 DVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSL 240

Query: 241 NTPARLHDRHRS---TDMSFDLGVGSGYGSSDAYSLQ----PTPRASNFNEMETTTAGNT 300
           NT  R  + + S   + M F  G  S +G +D YS+Q    PTPR SNF   E+    ++
Sbjct: 241 NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFE--ESCAMASS 300

Query: 301 PTWGRSPVGGRIFRQGSPAPAFGSVK---------------------------MVWESPG 360
           P +G  P GG      +P P F +                              VW S G
Sbjct: 301 PRFGYYP-GGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNG 360

Query: 361 S--------------REREGEGQGYKAVLPEKEISFRDSSMTPM--------PEEAAAEV 420
           S               E+ G+     A      IS    +  PM          E   EV
Sbjct: 361 SPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMNGDYGGEEESERVKEV 420

Query: 421 KSG--------------------------QEMPSALVMIRLILTVVGRKLSRNPNTYSSV 480
            +G                          + MP A VM RLIL +V RKL RNPNTYSS+
Sbjct: 421 PNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWRKLIRNPNTYSSL 480

Query: 481 LGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIG 518
           +GL+W+L++F+W V MP +++ SI I+SDAGLGMAMFSLGLFM +QPK+I CG   AT  
Sbjct: 481 IGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNSTATFA 540

BLAST of Sgr028322 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.9e-131
Identity = 289/604 (47.85%), Postives = 368/604 (60.93%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +FI ADTL KL +L LL +WA F  +GSL+W IT+FSL+TLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYG+ + SLMVQ+VVLQCIIWYTLLLFLFEYR A +LI  QFP    A+I  F+++ 
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSFKVES 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPD-SGLSSIGITPRASNLSNAEIFSI 240
           DV+SLDG D L T+++I + G++ V +R+S +S     G     +TPR SNL+ AEI+S+
Sbjct: 181 DVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSL 240

Query: 241 NTPARLHDRHRS---TDMSFDLGVGSGYGSSDAYSLQ----PTPRASNFNEMETTTAGNT 300
           NT  R  + + S   + M F  G  S +G +D YS+Q    PTPR SNF   E+    ++
Sbjct: 241 NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFE--ESCAMASS 300

Query: 301 PTWGRSPVGGRIFRQGSPAPAFGSVK---------------------------MVWESPG 360
           P +G  P GG      +P P F +                              VW S G
Sbjct: 301 PRFGYYP-GGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNG 360

Query: 361 S--REREG--------EGQGYKAVLPEKEISFRDSSMT--------PM--------PEEA 420
           S   +R G        E  G       KEI    S  T        PM          E 
Sbjct: 361 SPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNGENKAGPMNGDYGGEEESER 420

Query: 421 AAEVKSG--------------------------QEMPSALVMIRLILTVVGRKLSRNPNT 480
             EV +G                          + MP A VM RLIL +V RKL RNPNT
Sbjct: 421 VKEVPNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWRKLIRNPNT 480

Query: 481 YSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKR 518
           YSS++GL+W+L++F+W V MP +++ SI I+SDAGLGMAMFSLGLFM +QPK+I CG   
Sbjct: 481 YSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNST 540

BLAST of Sgr028322 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 461.5 bits (1186), Expect = 9.6e-130
Identity = 291/608 (47.86%), Postives = 360/608 (59.21%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MITA DFY VM AMVPLY AM++AY SVKWWKIFT +QC+GINRFVA+FAVP+LSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            NNPY M+ +F+ AD+L K+ VL LL LW      GSLDW ITLFSL+TLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L  MYG+ +  LMVQ+VVLQCIIWYTL+LFLFEYR A +LI  QFP   A +I    +D 
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFP-DTAGSIVSIHVDS 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPD------SGLSSIGITPRASNLSNA 240
           D++SLDGR  L TE+EI   G++ V +RRS +S  D       GLS+   TPR SNL+NA
Sbjct: 181 DIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA---TPRPSNLTNA 240

Query: 241 EIFSINT---PARLHDRHRSTD----MSFDLGVGSGYGSSDAY--SLQPTPRASNFNE-- 300
           EI+S+ +   P         TD    M+   G  S +G  +A   S  PTPR SN+ E  
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDG 300

Query: 301 --METTTAGNTPTWGR------------------------SP---VGGRIFRQGSPAPAF 360
              + T AG     GR                        SP    GG    +G+ AP  
Sbjct: 301 GPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGN-APVV 360

Query: 361 GSVK----------MVWESPGSREREGEGQG------------------YKAVLPE---- 420
           G  +           VW S  S   +  G G                   K  +P+    
Sbjct: 361 GGKRQDGNGRDLHMFVWSSSASPVSDVFGGGGGNHHADYSTATNDHQKDVKISVPQGNSN 420

Query: 421 -------KEISF--RDSSMTPMPEEAAAEVKS----GQEMPSALVMIRLILTVVGRKLSR 480
                  +E SF  +D     +  +    + +     + MP   VM RLIL +V RKL R
Sbjct: 421 DNQYVEREEFSFGNKDDDSKVLATDGGNNISNKTTQAKVMPPTSVMTRLILIMVWRKLIR 480

Query: 481 NPNTYSSVLGLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGC 518
           NPN+YSS+ G+ WSL+SFKW +EMP+L+  SI I+SDAGLGMAMFSLGLFM + P+II C
Sbjct: 481 NPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIAC 540

BLAST of Sgr028322 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 459.9 bits (1182), Expect = 2.8e-129
Identity = 281/599 (46.91%), Postives = 363/599 (60.60%), Query Frame = 0

Query: 1   MITAGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60
           MIT  D Y V+ A+VPLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPYQMDTKFILADTLSKLFVLLLLSLWAVFFTAGSLDWLITLFSLATLPNTLVMGIPL 120
            N+PY M+ +F+ ADTL K+ +L+LL+LWA     GSL+W+IT+FSL+TLPNTLVMGIPL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LNAMYGDSTQSLMVQLVVLQCIIWYTLLLFLFEYRAATVLIQNQFPGSAAAAITKFELDG 180
           L AMYG    SLMVQ+VVLQCIIWYTLLLFLFEYR A +LI  QFP    A+I  F+++ 
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFP-ETGASIVSFKVES 180

Query: 181 DVISLDGRDELLTESEIDNQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEIFSIN 240
           DV+SLDG D L T++EI N G++ V +R+S +S       S+ +TPR SNL+ AEI+S++
Sbjct: 181 DVVSLDGHDFLETDAEIGNDGKLHVTVRKSNASR-----RSLMMTPRPSNLTGAEIYSLS 240

Query: 241 TPARLHDRHRS---TDMSFDLGVGSGYGSSDAYSLQ----PTPRASNFNE-------MET 300
           +  R  + + S   + M F  G  S +G +D YS+Q    PTPR SNF E          
Sbjct: 241 STPRGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYN 300

Query: 301 TTAGNTPTWGRSPVGGRIFRQGS--------------------PAPAFGSVK----MVWE 360
            T  + P  G  P     F  G+                     + A    K     VW 
Sbjct: 301 NTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWS 360

Query: 361 SPGSR-------------EREGEGQGYKAVL----------------------PEKEISF 420
           S  S                E   QG K +                        E+EI  
Sbjct: 361 SSASPVSDVFGGGAGDNVATEQSEQGAKEIRMVVSDQPRKSGGDDIGGLDSGEGEREIEK 420

Query: 421 RDSSMTPMPEEAAAEVK---------SGQEMPSALVMIRLILTVVGRKLSRNPNTYSSVL 480
             + +  M   + AE++         +G  MP   VM RLIL +V RKL RNPNTYSS++
Sbjct: 421 ATAGLNKMGSNSTAELEAAGGDGGGNNGTHMPPTSVMTRLILIMVWRKLIRNPNTYSSLI 480

Query: 481 GLLWSLLSFKWKVEMPSLVKNSIKIISDAGLGMAMFSLGLFMGVQPKIIGCGGKRATIGM 518
           GL+W+L++++W V MP +++ SI I+SDAGLGMAMFSLGLFM +QPKII CG   AT  M
Sbjct: 481 GLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMFSLGLFMALQPKIIACGNSVATFAM 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897269.18.2e-23283.17auxin efflux carrier component 6 [Benincasa hispida][more]
XP_023525387.11.6e-22783.30auxin efflux carrier component 6 [Cucurbita pepo subsp. pepo][more]
XP_022981447.12.1e-22783.82auxin efflux carrier component 6 [Cucurbita maxima][more]
KAG6607665.11.1e-22582.73Auxin efflux carrier component 6, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022926200.11.3e-22482.34auxin efflux carrier component 6 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SQH61.6e-18265.49Auxin efflux carrier component 6 OS=Arabidopsis thaliana OX=3702 GN=PIN6 PE=2 SV... [more]
Q67UL33.7e-13450.43Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ94.5e-13249.31Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Q940Y55.5e-13047.85Auxin efflux carrier component 7 OS=Arabidopsis thaliana OX=3702 GN=PIN7 PE=1 SV... [more]
Q9C6B81.4e-12847.86Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1ITZ91.0e-22783.82Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111480565 PE=3 ... [more]
A0A6J1EKF96.1e-22582.34Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111433384 PE=... [more]
F6H0969.8e-21577.34Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=VIT_18s0001g15420 P... [more]
A0A5J5BUQ12.4e-21376.65Auxin efflux carrier component OS=Nyssa sinensis OX=561372 GN=F0562_002743 PE=3 ... [more]
A0A438HKK32.0e-21276.44Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN6_0 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G77110.11.2e-18365.49Auxin efflux carrier family protein [more]
AT1G23080.31.3e-13147.50Auxin efflux carrier family protein [more]
AT1G23080.13.9e-13147.85Auxin efflux carrier family protein [more]
AT1G73590.19.6e-13047.86Auxin efflux carrier family protein [more]
AT2G01420.12.8e-12946.91Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 10..519
e-value: 1.7E-156
score: 520.5
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..530
e-value: 9.1E-120
score: 397.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..295
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..517
NoneNo IPR availablePANTHERPTHR31752:SF56AUXIN EFFLUX CARRIER COMPONENT 6coord: 1..517

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr028322.1Sgr028322.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity