Sgr028296 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028296 vs. NCBI nr
Match: XP_022153687.1 (small RNA degrading nuclease 5 [Momordica charantia])

HSP 1 Score: 995.3 bits (2572), Expect = 2.8e-286
Identity = 503/566 (88.87%), Postives = 532/566 (93.99%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSSS S L+SE+E   NV  TKE SSFFDIYGTDAKAEVVFKTP+ NSTLSLQDVQGLV
Sbjct: 1   MPSSSASVLDSEDEAQLNVGKTKEKSSFFDIYGTDAKAEVVFKTPDSNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKV MVYLPGLDAA+Y+SQSKI PTFKKFCGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVAMVYLPGLDAALYMSQSKILPTFKKFCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SCVSDRMQTIDALLTCKVKRKR      LK S+PT++QERFG EKETP+SIKLPKELPFP
Sbjct: 121 SCVSDRMQTIDALLTCKVKRKRGANDVDLKKSDPTAEQERFGSEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DM+G+IVLDKLVKP NAIVDYNTRFSGIT EML+GVTT L+D QRDFLNLVHKETIL+GH
Sbjct: 241 DMQGEIVLDKLVKPFNAIVDYNTRFSGITSEMLSGVTTTLEDVQRDFLNLVHKETILIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALK+SHDLVVDTAVLYKHPRG SHK ALRILAKKFLSREIQQSGNGHDS EDA
Sbjct: 301 SLENDLMALKMSHDLVVDTAVLYKHPRGGSHKTALRILAKKFLSREIQQSGNGHDSTEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLT+LGESGK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTILGESGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSDEEALSRAKKEV+NE+VHF+WTQFSELNSYYKKQADDEERL+VKLAEM+SLLTCDTK
Sbjct: 421 VTSDEEALSRAKKEVINEKVHFIWTQFSELNSYYKKQADDEERLNVKLAEMMSLLTCDTK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVNRK++SRK+S ELREIL  MDSRIKNLY+SLPTNAMLIICTGHGDIALVQRLR+MLKE
Sbjct: 481 SVNRKKHSRKISPELREILTRMDSRIKNLYSSLPTNAMLIICTGHGDIALVQRLRRMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           Q+ET ICREKIVKILEELHAQAE  L
Sbjct: 541 QNETSICREKIVKILEELHAQAEVAL 566

BLAST of Sgr028296 vs. NCBI nr
Match: XP_022981433.1 (small RNA degrading nuclease 5-like [Cucurbita maxima])

HSP 1 Score: 983.8 bits (2542), Expect = 8.3e-283
Identity = 498/566 (87.99%), Postives = 532/566 (93.99%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSS  SAL+SEEEG SNV  TKE  SFFDIYGTDAKAE++FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSGVSALDSEEEGRSNVGKTKENPSFFDIYGTDAKAEILFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQ+KI PTFKK CGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQTKILPTFKKLCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRD  S  LK +N TS+QERFGPEKETP+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDANSVDLK-NNLTSEQERFGPEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAP+GS PYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPIGSSPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DMEGQ+VLDKLVKPSNAIVDYNTRFSGIT EML+ VTT L+D Q+ FLNLVHKET+L+GH
Sbjct: 241 DMEGQVVLDKLVKPSNAIVDYNTRFSGITFEMLSQVTTSLEDVQKVFLNLVHKETVLIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLV+DTAVLYKHPRG SHK ALRIL KKFLSREIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVIDTAVLYKHPRGGSHKSALRILTKKFLSREIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLT+LGE+GK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTILGENGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKEVMNERVHF+WTQFSELNSY+KKQ DDEERL+VKLAE+LSLLTCD+K
Sbjct: 421 VTSDDEALSRAKKEVMNERVHFIWTQFSELNSYHKKQVDDEERLNVKLAELLSLLTCDSK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVN+KRNSRK+S ELREIL+ MDSRIK+LYTSLPTNAMLI+CTGHGDIALVQRLRKMLKE
Sbjct: 481 SVNKKRNSRKISVELREILSRMDSRIKDLYTSLPTNAMLIVCTGHGDIALVQRLRKMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET I REKIVKILE+LHAQAE  L
Sbjct: 541 QSETMISREKIVKILEDLHAQAEVAL 565

BLAST of Sgr028296 vs. NCBI nr
Match: XP_022926206.1 (small RNA degrading nuclease 5-like [Cucurbita moschata])

HSP 1 Score: 983.0 bits (2540), Expect = 1.4e-282
Identity = 498/566 (87.99%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSS  SAL+SEEEG S+V  TKE  SFFDIYGTDAKAE++FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSGVSALDSEEEGRSDVGKTKENPSFFDIYGTDAKAEILFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQ+KI PTFKK CGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQTKILPTFKKLCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRD  S  LK +N TS+QER GPEKETP+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDANSVDLK-NNLTSEQERLGPEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAP+GS PYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPIGSSPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DMEGQ+VLDKLVKPSNAIVDYNTRFSGIT EML+ VTT L+D Q+ FLNLVHKET+L+GH
Sbjct: 241 DMEGQVVLDKLVKPSNAIVDYNTRFSGITSEMLSQVTTSLEDVQKVFLNLVHKETVLIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLV+DTAVLYKHPRG SHK ALRIL KKFLSREIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVIDTAVLYKHPRGGSHKSALRILTKKFLSREIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLTVLGE+GK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTVLGENGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKEVMNERVHF+WTQFSELNSY+KKQ DDEERL+VKLAE+LSLLTCD+K
Sbjct: 421 VTSDDEALSRAKKEVMNERVHFIWTQFSELNSYHKKQVDDEERLNVKLAELLSLLTCDSK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVN+KRNSRK+S ELREIL+ MD RIK+LYTSLPTNAMLI+CTGHGDIALVQRLRKMLKE
Sbjct: 481 SVNKKRNSRKISVELREILSRMDYRIKDLYTSLPTNAMLIVCTGHGDIALVQRLRKMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET ICREKIVKILEELHAQAE  L
Sbjct: 541 QSETMICREKIVKILEELHAQAEVAL 565

BLAST of Sgr028296 vs. NCBI nr
Match: XP_023525579.1 (small RNA degrading nuclease 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.9 bits (2524), Expect = 1.0e-280
Identity = 494/566 (87.28%), Postives = 529/566 (93.46%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSS  SAL+SEEEG SNV  TKE  SFFDIYGTDAKAE++FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSGVSALDSEEEGRSNVGKTKENPSFFDIYGTDAKAEILFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQ+KI  TFKK CGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQTKILSTFKKLCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRD  S  LK +N TS+QER  PEKETP+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDANSVDLK-NNLTSEQERLDPEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAP+GS PYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPIGSSPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DMEGQ+VLDKLVKPSNAIVDYNTRFSGIT E+L+ VTT L+D Q+ FLNLVHKET+L+GH
Sbjct: 241 DMEGQVVLDKLVKPSNAIVDYNTRFSGITFELLSQVTTSLEDVQKVFLNLVHKETVLIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLV+DTAVLYKHPRG SHK ALRIL KKFLSREIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVIDTAVLYKHPRGGSHKSALRILTKKFLSREIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLT+LGE+GK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTILGENGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKEVMNERVHF+WTQFSELNSY+KKQ DDEERL+VKLAE+LSLLTCD+K
Sbjct: 421 VTSDDEALSRAKKEVMNERVHFIWTQFSELNSYHKKQVDDEERLNVKLAELLSLLTCDSK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           S N+KRNSRK+S ELREIL+ MDSRIK+LYTSLPTNAMLI+CTGHGDIALVQRLRKMLKE
Sbjct: 481 SANKKRNSRKISVELREILSRMDSRIKDLYTSLPTNAMLIVCTGHGDIALVQRLRKMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET ICREKIVK+LEELHAQAE  L
Sbjct: 541 QSETMICREKIVKMLEELHAQAEVAL 565

BLAST of Sgr028296 vs. NCBI nr
Match: XP_038886453.1 (small RNA degrading nuclease 5 [Benincasa hispida])

HSP 1 Score: 957.6 bits (2474), Expect = 6.4e-275
Identity = 488/566 (86.22%), Postives = 525/566 (92.76%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSSS SAL+  +EG SN   T+E SSFFDIYGTDAKAEV+FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSSVSALDCVDEGKSNAGKTQEKSSFFDIYGTDAKAEVLFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMP WVFIKNKPLIPKVVMVYLPGLDAA+Y+SQSKI PTFK+FCG+PRAVLAL
Sbjct: 61  TWVLAEGFMPQWVFIKNKPLIPKVVMVYLPGLDAALYMSQSKILPTFKRFCGSPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRDE S  +K +  TS+ E  G EKE P+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDENSVDIK-NKLTSEPEISGLEKEAPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           ITYYTLTAK LEDNGY FNQPGFI TLPAP+GS P EMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 ITYYTLTAKALEDNGYPFNQPGFIQTLPAPVGSSPNEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           D+EG++VLDKLVKPSNAIVDYNTRFSGITCEML+ VTT LKD QRDFLNLVHKET+LVGH
Sbjct: 241 DIEGRVVLDKLVKPSNAIVDYNTRFSGITCEMLSEVTTSLKDVQRDFLNLVHKETVLVGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLVVDTAVLYKHPRG SHK ALRILAK+FLS+EIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVVDTAVLYKHPRGGSHKSALRILAKRFLSKEIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGP+FGSPPSLIRKKLL +LGESGK SSFID++STVKRYSSES+HAFP
Sbjct: 361 RATMELALLKIKHGPDFGSPPSLIRKKLLNILGESGKTSSFIDNISTVKRYSSESTHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKE MNERVHF+WTQFSELNSY+KKQADDEERL+VKLAE+LSLLTCDTK
Sbjct: 421 VTSDDEALSRAKKEAMNERVHFIWTQFSELNSYHKKQADDEERLNVKLAELLSLLTCDTK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           +VN+KRNS K+SAELREILA MDSRI +LYTSLPTNAMLIICTGHGDIALVQRLRKML+E
Sbjct: 481 TVNKKRNSPKISAELREILARMDSRINDLYTSLPTNAMLIICTGHGDIALVQRLRKMLQE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET ICREKIVKILEELHAQAE  L
Sbjct: 541 QSETSICREKIVKILEELHAQAEVAL 565

BLAST of Sgr028296 vs. ExPASy Swiss-Prot
Match: Q8L7M4 (Small RNA degrading nuclease 5 OS=Arabidopsis thaliana OX=3702 GN=SDN5 PE=2 SV=2)

HSP 1 Score: 677.9 bits (1748), Expect = 1.3e-193
Identity = 343/541 (63.40%), Postives = 433/541 (80.04%), Query Frame = 0

Query: 26  SSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV 85
           +SFFDIYG +AKAE+ FK+PE  +TL+LQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV
Sbjct: 28  NSFFDIYGPEAKAELDFKSPE--TTLNLQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV 87

Query: 86  MVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLALSCVSDRMQTIDALLTCKVKRKRDEI 145
           ++YLPGLDAA+YLS SK   + K  CGNP A+LALSCV D M+TID +LTCK ++K+   
Sbjct: 88  LLYLPGLDAALYLSHSKTLSSLKSCCGNPMALLALSCVVDEMRTIDTILTCKGRKKKTVT 147

Query: 146 SAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFPITYYTLTAKELEDNGYLFNQPGFIH 205
           S+V      +S +      K     ++L K++PFP++YYTL+ KE+E NGY F +     
Sbjct: 148 SSVEPPPLVSSPEACNLMGKS---FVELTKDIPFPVSYYTLSQKEMEQNGYTFEKLELTP 207

Query: 206 TLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLVDMEGQIVLDKLVKPSNAIVDYNTRF 265
           TLPAP GS P E++ALDCEMC+T EG ELTR+TLVD++GQ++LDKLV P+N I DYNTR+
Sbjct: 208 TLPAPSGSCPPEIVALDCEMCITKEGLELTRVTLVDIQGQVLLDKLVMPTNPITDYNTRY 267

Query: 266 SGITCEMLNGVTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKH 325
           SGIT  M+ GVTT LKD Q +FL LV KETILVGHSLENDL++LKISH+LV+DTAVLYKH
Sbjct: 268 SGITAVMMEGVTTTLKDIQEEFLKLVFKETILVGHSLENDLLSLKISHNLVIDTAVLYKH 327

Query: 326 PRGASHKVALRILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPSLIR 385
           P G S+K  LRILAKKFL+REIQ+S +GHDS EDA+A M+LALLKI+HGP+FGSPP +IR
Sbjct: 328 PHGRSYKTKLRILAKKFLAREIQESESGHDSAEDAKAAMDLALLKIKHGPDFGSPPEVIR 387

Query: 386 KKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFPVTSDEEALSRAKKEVMNERVHFVWT 445
           KKLL VL ESGK +S ID+++ VKRY+SESS+A PV+SD+EALS+A KEV N+R  FVWT
Sbjct: 388 KKLLAVLNESGKTTSIIDNINIVKRYASESSNAIPVSSDDEALSKAVKEVKNKRSQFVWT 447

Query: 446 QFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTKSVNRKRNSRKLSAELREILACMDSR 505
           QFSELN++++ +ADD ++L+ +LAEM+SLLTC+ KS  +K N   +S E +EIL  M+ R
Sbjct: 448 QFSELNAHFQSRADDPQKLNSRLAEMISLLTCNKKSGLKKSN---VSPETKEILKKMNER 507

Query: 506 IKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKEQSETRICREKIVKILEELHAQAETE 565
           +++LY +LPTNAM I+CTGHGD ++V R+RKMLK++ E    REK+VK+LEEL AQAE  
Sbjct: 508 VQSLYAALPTNAMFIVCTGHGDTSIVHRVRKMLKDEDEIGFDREKLVKVLEELQAQAEVA 560

Query: 566 L 567
           L
Sbjct: 568 L 560

BLAST of Sgr028296 vs. ExPASy Swiss-Prot
Match: O94443 (Uncharacterized exonuclease C637.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC637.09 PE=3 SV=2)

HSP 1 Score: 205.7 bits (522), Expect = 1.9e-51
Identity = 157/539 (29.13%), Postives = 266/539 (49.35%), Query Frame = 0

Query: 51  LSLQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKF 110
           L + D+Q LV W LA+G  PSWV ++NK +I + V++ +PGL+ + +         F+  
Sbjct: 100 LKISDLQELVFWCLADGQAPSWVLVRNKQMIHRAVILLVPGLEPSQF--------GFQPV 159

Query: 111 CGNPRAVLALSCVS--------DRMQTIDALLTCKVKRKRDEISAVLKT--SNPTSDQER 170
            GN  + L  + ++        D  +  D     +    R  + + +     +P S++++
Sbjct: 160 RGNKHSFLLPNLLNENGPIQLPDFCEVFDRAWPTRSPGDRFRVFSPVNAFLQSPLSNEQK 219

Query: 171 FGPEKETPLSIKLPKELPFPITYYTLTAKELE-----DNGYLFNQP-------GFIHTLP 230
              +KET       K   + ++Y +    E         G    QP       G  H+ P
Sbjct: 220 KKRDKETRAMASFSKPSDYLMSYESFIEDEYPLHPTVMKGEEVTQPSGWVASAGDFHSPP 279

Query: 231 APLGSLPYEMLALDCEMCVTCEGFELTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGI 290
                +  ++LA+DCEM  T  G E+ R+T+VDM+ +++ D+ VKP + + DY T++SGI
Sbjct: 280 -----INPKILAIDCEMVRTENGLEIARVTIVDMKSEVIYDEFVKPESPVTDYVTQYSGI 339

Query: 291 TCEMLNGVTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKHPRG 350
           T E L  VTT+L D Q      V   T+L+GHSL +DL  LK +H  ++DTA +Y H RG
Sbjct: 340 TEEKLRNVTTVLSDVQSYLKKTVDNNTVLLGHSLNSDLNCLKFTHPHIIDTANIYNHTRG 399

Query: 351 ASHKVALRILAKKFLSREIQQSGN-GHDSIEDARATMELALLKIRHGPEFGSPPSLIRKK 410
              K +L+ LA K+L REIQ++G  GHDS EDA A ++L  LK+++GP FG    L  + 
Sbjct: 400 PPSKPSLKWLATKWLRREIQKAGALGHDSAEDALACVDLLKLKVKNGPAFG----LFNQD 459

Query: 411 LLTVLGESGK-----MSSFIDDVSTVKRYSSESSH-AFPVTSDEEALSRAKKEVMNERVH 470
             ++     +     +   I D    +    +++H +    +D+E +S      +++  +
Sbjct: 460 FESIFHRLSRQQPTPLIGAIADYGNPESCIGKAAHKSVSCANDDEVVSAVVS--LSDMHN 519

Query: 471 FVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTKSVNRKRNSRKLSAELREILAC 530
           FVW +F EL       A+   +      E  S    +  SV   + +   SA     L  
Sbjct: 520 FVWGRFRELEHAAMWNANRNTK-----QENNSDTDTENDSVEEDQVTSYSSA-----LER 579

Query: 531 MDSRIKNLYTSLPTNAMLIICTGHG---DIALVQRLRKMLKEQSETRICREKIVKILEE 558
            + RI+ LY SLP  ++L++ TG G   +++ +  +R+  +++ +T+   E  VK  +E
Sbjct: 580 FNRRIRLLYDSLPKGSLLLLYTGTGNPIEMSKLNAIRQQFRKEYQTKKWDELSVKWTDE 609

BLAST of Sgr028296 vs. ExPASy Swiss-Prot
Match: P53331 (RNA exonuclease 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RNH70 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 5.6e-40
Identity = 111/342 (32.46%), Postives = 178/342 (52.05%), Query Frame = 0

Query: 51  LSLQDVQGLVTWVL-AEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIF----- 110
           + ++DV+ +  ++L AE   P W+ I N+  + K++++++PGL    + +    F     
Sbjct: 65  IGIKDVRDMTQYLLQAENNSPKWIDICNRSSLQKMIVLFIPGLQPDDFENGKNTFNEISD 124

Query: 111 PTFKKFCGNPRAVLALSCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPE 170
             FK   G          ++    T   +            ++ +      S  E+    
Sbjct: 125 DNFKYIPGE---------IASTFHTFPVMAPGSKMTLFSPYNSFINVG--LSKMEKINKL 184

Query: 171 KETPLSIKLPKELPFPITYYTLTAKELEDNGYLFN------QPGFIHTLPAPLGSLPYEM 230
           KE      L K+    I    L+ ++L  N Y  +         ++ T+    G     +
Sbjct: 185 KE------LQKKKKITINDLVLSEQQLVANDYPLDSGDTNFDTDWVQTVDFTHGG--SHI 244

Query: 231 LALDCEMCVTCEGFELTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLN-GVT 290
            ALDCEMC++ +G  LTRI+LV+ + +++ ++LVKP   IVDY TR+SGIT E L  G  
Sbjct: 245 FALDCEMCLSEQGLVLTRISLVNFDNEVIYEELVKPDVPIVDYLTRYSGITEEKLTVGAK 304

Query: 291 TILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRI 350
             L++ Q+D L ++ +  IL+GHSL+NDL  +K+ H LVVDTA++Y H  G   K +L+ 
Sbjct: 305 KTLREVQKDLLKIISRSDILIGHSLQNDLKVMKLKHPLVVDTAIIYHHKAGDPFKPSLKY 364

Query: 351 LAKKFLSREIQQSGNG-HDSIEDARATMELALLKIRHGPEFG 379
           L++ FL++ IQ   NG HDS+EDARA +EL  LKI +G  FG
Sbjct: 365 LSETFLNKSIQ---NGEHDSVEDARACLELTKLKILNGLAFG 384

BLAST of Sgr028296 vs. ExPASy Swiss-Prot
Match: Q8IX06 (Putative exonuclease GOR OS=Homo sapiens OX=9606 GN=REXO1L1P PE=5 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 1.5e-37
Identity = 86/186 (46.24%), Postives = 117/186 (62.90%), Query Frame = 0

Query: 188 AKELEDNGYLFNQPGFIHTLPAPLGSLPYE-MLALDCEMCVTCEGFELTRITLVDMEGQI 247
           AK+   +G   +  GF+ T    L    Y  + ALDCEMC T  G ELTR+T+VD + ++
Sbjct: 470 AKQHVRDGRKESLDGFVETFKKELSRDAYPGIYALDCEMCYTTHGLELTRVTVVDADMRV 529

Query: 248 VLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGHSLENDL 307
           V D  VKP N IVDYNTRFSG+T   +   +  L   Q   L+    +TIL+GHSLE+DL
Sbjct: 530 VYDTFVKPDNEIVDYNTRFSGVTEADVAKTSITLPQVQAILLSFFSAQTILIGHSLESDL 589

Query: 308 MALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDARATMEL 367
           +ALK+ H  VVDTAVL+ H  G  +K +LR LA  +L++ IQ S +GH+S EDA A ++L
Sbjct: 590 LALKLIHSTVVDTAVLFPHYLGFPYKRSLRNLAADYLAQIIQDSQDGHNSSEDASACLQL 649

Query: 368 ALLKIR 373
            + K+R
Sbjct: 650 VMWKVR 655

BLAST of Sgr028296 vs. ExPASy Swiss-Prot
Match: P48778 (Exonuclease GOR OS=Pan troglodytes OX=9598 GN=REXO1L1 PE=2 SV=3)

HSP 1 Score: 158.3 bits (399), Expect = 3.4e-37
Identity = 85/186 (45.70%), Postives = 116/186 (62.37%), Query Frame = 0

Query: 188 AKELEDNGYLFNQPGFIHTLPAPLGSLPYE-MLALDCEMCVTCEGFELTRITLVDMEGQI 247
           AK+   +G   +  GF+ T    L    Y  + ALDCEMC T  G ELTR+T+VD + ++
Sbjct: 376 AKQHVRDGRKDSLDGFVETFKKELSRDAYPGIYALDCEMCYTTHGLELTRVTVVDADMRV 435

Query: 248 VLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGHSLENDL 307
           V D  VKP N IVDYNTRFSG+T   +   +  L   Q   L+    +TIL+GHSLE+DL
Sbjct: 436 VYDTFVKPDNEIVDYNTRFSGVTEADVAKTSITLPQVQAILLSFFSAQTILIGHSLESDL 495

Query: 308 MALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDARATMEL 367
           +ALK+ H  V+DTAVL+ H  G  +K +LR LA  +L + IQ S +GH+S EDA A ++L
Sbjct: 496 LALKLIHSTVLDTAVLFPHYLGFPYKRSLRNLAADYLGQIIQDSQDGHNSSEDANACLQL 555

Query: 368 ALLKIR 373
            + K+R
Sbjct: 556 VMWKVR 561

BLAST of Sgr028296 vs. ExPASy TrEMBL
Match: A0A6J1DJM2 (small RNA degrading nuclease 5 OS=Momordica charantia OX=3673 GN=LOC111021144 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.3e-286
Identity = 503/566 (88.87%), Postives = 532/566 (93.99%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSSS S L+SE+E   NV  TKE SSFFDIYGTDAKAEVVFKTP+ NSTLSLQDVQGLV
Sbjct: 1   MPSSSASVLDSEDEAQLNVGKTKEKSSFFDIYGTDAKAEVVFKTPDSNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKV MVYLPGLDAA+Y+SQSKI PTFKKFCGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVAMVYLPGLDAALYMSQSKILPTFKKFCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SCVSDRMQTIDALLTCKVKRKR      LK S+PT++QERFG EKETP+SIKLPKELPFP
Sbjct: 121 SCVSDRMQTIDALLTCKVKRKRGANDVDLKKSDPTAEQERFGSEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DM+G+IVLDKLVKP NAIVDYNTRFSGIT EML+GVTT L+D QRDFLNLVHKETIL+GH
Sbjct: 241 DMQGEIVLDKLVKPFNAIVDYNTRFSGITSEMLSGVTTTLEDVQRDFLNLVHKETILIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALK+SHDLVVDTAVLYKHPRG SHK ALRILAKKFLSREIQQSGNGHDS EDA
Sbjct: 301 SLENDLMALKMSHDLVVDTAVLYKHPRGGSHKTALRILAKKFLSREIQQSGNGHDSTEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLT+LGESGK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTILGESGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSDEEALSRAKKEV+NE+VHF+WTQFSELNSYYKKQADDEERL+VKLAEM+SLLTCDTK
Sbjct: 421 VTSDEEALSRAKKEVINEKVHFIWTQFSELNSYYKKQADDEERLNVKLAEMMSLLTCDTK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVNRK++SRK+S ELREIL  MDSRIKNLY+SLPTNAMLIICTGHGDIALVQRLR+MLKE
Sbjct: 481 SVNRKKHSRKISPELREILTRMDSRIKNLYSSLPTNAMLIICTGHGDIALVQRLRRMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           Q+ET ICREKIVKILEELHAQAE  L
Sbjct: 541 QNETSICREKIVKILEELHAQAEVAL 566

BLAST of Sgr028296 vs. ExPASy TrEMBL
Match: A0A6J1J229 (small RNA degrading nuclease 5-like OS=Cucurbita maxima OX=3661 GN=LOC111480556 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 4.0e-283
Identity = 498/566 (87.99%), Postives = 532/566 (93.99%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSS  SAL+SEEEG SNV  TKE  SFFDIYGTDAKAE++FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSGVSALDSEEEGRSNVGKTKENPSFFDIYGTDAKAEILFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQ+KI PTFKK CGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQTKILPTFKKLCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRD  S  LK +N TS+QERFGPEKETP+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDANSVDLK-NNLTSEQERFGPEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAP+GS PYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPIGSSPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DMEGQ+VLDKLVKPSNAIVDYNTRFSGIT EML+ VTT L+D Q+ FLNLVHKET+L+GH
Sbjct: 241 DMEGQVVLDKLVKPSNAIVDYNTRFSGITFEMLSQVTTSLEDVQKVFLNLVHKETVLIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLV+DTAVLYKHPRG SHK ALRIL KKFLSREIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVIDTAVLYKHPRGGSHKSALRILTKKFLSREIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLT+LGE+GK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTILGENGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKEVMNERVHF+WTQFSELNSY+KKQ DDEERL+VKLAE+LSLLTCD+K
Sbjct: 421 VTSDDEALSRAKKEVMNERVHFIWTQFSELNSYHKKQVDDEERLNVKLAELLSLLTCDSK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVN+KRNSRK+S ELREIL+ MDSRIK+LYTSLPTNAMLI+CTGHGDIALVQRLRKMLKE
Sbjct: 481 SVNKKRNSRKISVELREILSRMDSRIKDLYTSLPTNAMLIVCTGHGDIALVQRLRKMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET I REKIVKILE+LHAQAE  L
Sbjct: 541 QSETMISREKIVKILEDLHAQAEVAL 565

BLAST of Sgr028296 vs. ExPASy TrEMBL
Match: A0A6J1EE80 (small RNA degrading nuclease 5-like OS=Cucurbita moschata OX=3662 GN=LOC111433387 PE=4 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 6.9e-283
Identity = 498/566 (87.99%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETSSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLV 60
           MPSS  SAL+SEEEG S+V  TKE  SFFDIYGTDAKAE++FKTPE NSTLSLQDVQGLV
Sbjct: 1   MPSSGVSALDSEEEGRSDVGKTKENPSFFDIYGTDAKAEILFKTPESNSTLSLQDVQGLV 60

Query: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLAL 120
           TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQ+KI PTFKK CGNPRAVLAL
Sbjct: 61  TWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQTKILPTFKKLCGNPRAVLAL 120

Query: 121 SCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFP 180
           SC+SDRMQTIDALLTCKVKRKRD  S  LK +N TS+QER GPEKETP+SIKLPKELPFP
Sbjct: 121 SCISDRMQTIDALLTCKVKRKRDANSVDLK-NNLTSEQERLGPEKETPISIKLPKELPFP 180

Query: 181 ITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLV 240
           +TYYTLTAKELEDNGY FNQPGFIHTLPAP+GS PYEMLALDCEMCVTCEGFELTRITLV
Sbjct: 181 VTYYTLTAKELEDNGYPFNQPGFIHTLPAPIGSSPYEMLALDCEMCVTCEGFELTRITLV 240

Query: 241 DMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETILVGH 300
           DMEGQ+VLDKLVKPSNAIVDYNTRFSGIT EML+ VTT L+D Q+ FLNLVHKET+L+GH
Sbjct: 241 DMEGQVVLDKLVKPSNAIVDYNTRFSGITSEMLSQVTTSLEDVQKVFLNLVHKETVLIGH 300

Query: 301 SLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSIEDA 360
           SLENDLMALKISHDLV+DTAVLYKHPRG SHK ALRIL KKFLSREIQQSGNGHDSIEDA
Sbjct: 301 SLENDLMALKISHDLVIDTAVLYKHPRGGSHKSALRILTKKFLSREIQQSGNGHDSIEDA 360

Query: 361 RATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFP 420
           RATMELALLKI+HGPEFGSPPSLIRKKLLTVLGE+GK SSFIDDVSTVKRYSSESSHAFP
Sbjct: 361 RATMELALLKIKHGPEFGSPPSLIRKKLLTVLGENGKTSSFIDDVSTVKRYSSESSHAFP 420

Query: 421 VTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTK 480
           VTSD+EALSRAKKEVMNERVHF+WTQFSELNSY+KKQ DDEERL+VKLAE+LSLLTCD+K
Sbjct: 421 VTSDDEALSRAKKEVMNERVHFIWTQFSELNSYHKKQVDDEERLNVKLAELLSLLTCDSK 480

Query: 481 SVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKE 540
           SVN+KRNSRK+S ELREIL+ MD RIK+LYTSLPTNAMLI+CTGHGDIALVQRLRKMLKE
Sbjct: 481 SVNKKRNSRKISVELREILSRMDYRIKDLYTSLPTNAMLIVCTGHGDIALVQRLRKMLKE 540

Query: 541 QSETRICREKIVKILEELHAQAETEL 567
           QSET ICREKIVKILEELHAQAE  L
Sbjct: 541 QSETMICREKIVKILEELHAQAEVAL 565

BLAST of Sgr028296 vs. ExPASy TrEMBL
Match: A0A6J1FAZ1 (small RNA degrading nuclease 5-like OS=Cucurbita moschata OX=3662 GN=LOC111443672 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 9.9e-274
Identity = 484/569 (85.06%), Postives = 526/569 (92.44%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETS---SFFDIYGTDAKAEVVFKTPECNSTLSLQDVQ 60
           MPSSS SAL+++EEG SNV  T+E     SFFDIYGTDAKAEV+FKTPE NSTLSLQDVQ
Sbjct: 1   MPSSSVSALDAKEEGKSNVGNTEEEEDKPSFFDIYGTDAKAEVLFKTPESNSTLSLQDVQ 60

Query: 61  GLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAV 120
           GLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQSKI PTFK+FCG+PR V
Sbjct: 61  GLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQSKILPTFKRFCGSPRPV 120

Query: 121 LALSCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKLPKEL 180
           LALSC+SDRMQTIDALLTCKVKRKRD  S  LK +  TS+QE FGPEK++P+SIKLPKEL
Sbjct: 121 LALSCISDRMQTIDALLTCKVKRKRDANSVDLK-NKLTSEQEIFGPEKDSPISIKLPKEL 180

Query: 181 PFPITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFELTRI 240
           PFP+TYYTLTAKELEDNGY FNQPGFI TLPAP+GS PYEMLALDCEMCVTCEGFELTRI
Sbjct: 181 PFPVTYYTLTAKELEDNGYPFNQPGFIQTLPAPMGSSPYEMLALDCEMCVTCEGFELTRI 240

Query: 241 TLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHKETIL 300
           TLVDM GQIVLDKLVKPSNAIVDYNTRFSGITCEMLN VTT L+D Q+DFLNLVHKET+L
Sbjct: 241 TLVDMAGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNEVTTSLEDVQKDFLNLVHKETVL 300

Query: 301 VGHSLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNGHDSI 360
           VGHSLENDLMALKISHDLVVDTAVLYKHPRG SHK ALRILAK+FLSREIQQS NGH+S+
Sbjct: 301 VGHSLENDLMALKISHDLVVDTAVLYKHPRGGSHKSALRILAKRFLSREIQQSSNGHNSV 360

Query: 361 EDARATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSSESSH 420
           EDARATMELALLKI+HGPEFGSPPSL+RKKLL +LGESGK SSFIDDVSTVKRYSSESSH
Sbjct: 361 EDARATMELALLKIKHGPEFGSPPSLVRKKLLNILGESGKTSSFIDDVSTVKRYSSESSH 420

Query: 421 AFPVTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLSLLTC 480
           AFPVTSD+EAL++AKKE MNERVHFVWTQFSELNSY+KKQADD+ERL+VKLAE+LSL+TC
Sbjct: 421 AFPVTSDDEALAKAKKEAMNERVHFVWTQFSELNSYHKKQADDDERLNVKLAELLSLVTC 480

Query: 481 DTKSVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQRLRKM 540
           DTKSV++KRNS K+  ELREILA +DSRI +LY SLPTNAMLI+CTGHGDIALVQRLRKM
Sbjct: 481 DTKSVSKKRNSPKICVELREILAHIDSRIDSLYASLPTNAMLIMCTGHGDIALVQRLRKM 540

Query: 541 LKEQSETRICREKIVKILEELHAQAETEL 567
           L EQSET ICR+K+VKILEELHAQAE  L
Sbjct: 541 LVEQSETSICRDKVVKILEELHAQAEVAL 568

BLAST of Sgr028296 vs. ExPASy TrEMBL
Match: A0A6J1IFC2 (small RNA degrading nuclease 5-like OS=Cucurbita maxima OX=3661 GN=LOC111473789 PE=4 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 1.2e-271
Identity = 482/573 (84.12%), Postives = 523/573 (91.27%), Query Frame = 0

Query: 1   MPSSSGSALNSEEEGNSNVCITKETS-------SFFDIYGTDAKAEVVFKTPECNSTLSL 60
           MPSSS SAL+++EEG SNV  T+E         SFFDIYGTDAKAEV+FKTPE NSTLSL
Sbjct: 1   MPSSSVSALDAKEEGKSNVGNTEEEEEEEEEKPSFFDIYGTDAKAEVLFKTPESNSTLSL 60

Query: 61  QDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAAMYLSQSKIFPTFKKFCGN 120
           QDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAA+Y+SQSKI PTFK+FCG+
Sbjct: 61  QDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVVMVYLPGLDAALYMSQSKILPTFKRFCGS 120

Query: 121 PRAVLALSCVSDRMQTIDALLTCKVKRKRDEISAVLKTSNPTSDQERFGPEKETPLSIKL 180
           PR VLALSC+SDRMQTIDALLTCKVKRKRD  S  LK +  TS+QE FGPEK++P+SIKL
Sbjct: 121 PRPVLALSCISDRMQTIDALLTCKVKRKRDANSVGLK-NKMTSEQEIFGPEKDSPISIKL 180

Query: 181 PKELPFPITYYTLTAKELEDNGYLFNQPGFIHTLPAPLGSLPYEMLALDCEMCVTCEGFE 240
           PKELPFP+TYYTLTAKELEDNGY FNQPGFI TLPAP+GS PYEMLALDCEMCVTCEGFE
Sbjct: 181 PKELPFPVTYYTLTAKELEDNGYPFNQPGFIQTLPAPMGSSPYEMLALDCEMCVTCEGFE 240

Query: 241 LTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGVTTILKDAQRDFLNLVHK 300
           LTRITLVDM GQIVLDKLVKPSNAIV+YNTRFSGITCEMLN VTT L+D Q+D LNLVHK
Sbjct: 241 LTRITLVDMAGQIVLDKLVKPSNAIVNYNTRFSGITCEMLNEVTTSLEDVQKDLLNLVHK 300

Query: 301 ETILVGHSLENDLMALKISHDLVVDTAVLYKHPRGASHKVALRILAKKFLSREIQQSGNG 360
           ET+LVGHSLENDLMALKISHD VVDTAVLYKHPRG SHK ALRILAK+FLSREIQQS NG
Sbjct: 301 ETVLVGHSLENDLMALKISHDRVVDTAVLYKHPRGGSHKSALRILAKRFLSREIQQSSNG 360

Query: 361 HDSIEDARATMELALLKIRHGPEFGSPPSLIRKKLLTVLGESGKMSSFIDDVSTVKRYSS 420
           HDS+EDARATMELALLKI+HGPEFGSPPSL+RKKLL +LGESGK SSFIDDVSTVKRYSS
Sbjct: 361 HDSVEDARATMELALLKIKHGPEFGSPPSLVRKKLLNILGESGKTSSFIDDVSTVKRYSS 420

Query: 421 ESSHAFPVTSDEEALSRAKKEVMNERVHFVWTQFSELNSYYKKQADDEERLSVKLAEMLS 480
           ESSHAFPV SD+EAL++AKKE MN+RVHFVWTQFSELNSY+KKQADDEERL+VKLAE+LS
Sbjct: 421 ESSHAFPVISDDEALAKAKKEAMNQRVHFVWTQFSELNSYHKKQADDEERLNVKLAELLS 480

Query: 481 LLTCDTKSVNRKRNSRKLSAELREILACMDSRIKNLYTSLPTNAMLIICTGHGDIALVQR 540
           L+TCDTKSV++KRNS K+  ELREILA +DSRI +LY SLPTNAMLIICTGHGDIALVQR
Sbjct: 481 LVTCDTKSVSKKRNSPKICVELREILARIDSRIDSLYASLPTNAMLIICTGHGDIALVQR 540

Query: 541 LRKMLKEQSETRICREKIVKILEELHAQAETEL 567
           LRKML EQSET ICR+K+VKILEELHAQAE  L
Sbjct: 541 LRKMLVEQSETSICRDKVVKILEELHAQAEVAL 572

BLAST of Sgr028296 vs. TAIR 10
Match: AT5G25800.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 677.9 bits (1748), Expect = 9.1e-195
Identity = 343/541 (63.40%), Postives = 433/541 (80.04%), Query Frame = 0

Query: 26  SSFFDIYGTDAKAEVVFKTPECNSTLSLQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV 85
           +SFFDIYG +AKAE+ FK+PE  +TL+LQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV
Sbjct: 28  NSFFDIYGPEAKAELDFKSPE--TTLNLQDVQGLVTWVLAEGFMPSWVFIKNKPLIPKVV 87

Query: 86  MVYLPGLDAAMYLSQSKIFPTFKKFCGNPRAVLALSCVSDRMQTIDALLTCKVKRKRDEI 145
           ++YLPGLDAA+YLS SK   + K  CGNP A+LALSCV D M+TID +LTCK ++K+   
Sbjct: 88  LLYLPGLDAALYLSHSKTLSSLKSCCGNPMALLALSCVVDEMRTIDTILTCKGRKKKTVT 147

Query: 146 SAVLKTSNPTSDQERFGPEKETPLSIKLPKELPFPITYYTLTAKELEDNGYLFNQPGFIH 205
           S+V      +S +      K     ++L K++PFP++YYTL+ KE+E NGY F +     
Sbjct: 148 SSVEPPPLVSSPEACNLMGKS---FVELTKDIPFPVSYYTLSQKEMEQNGYTFEKLELTP 207

Query: 206 TLPAPLGSLPYEMLALDCEMCVTCEGFELTRITLVDMEGQIVLDKLVKPSNAIVDYNTRF 265
           TLPAP GS P E++ALDCEMC+T EG ELTR+TLVD++GQ++LDKLV P+N I DYNTR+
Sbjct: 208 TLPAPSGSCPPEIVALDCEMCITKEGLELTRVTLVDIQGQVLLDKLVMPTNPITDYNTRY 267

Query: 266 SGITCEMLNGVTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKH 325
           SGIT  M+ GVTT LKD Q +FL LV KETILVGHSLENDL++LKISH+LV+DTAVLYKH
Sbjct: 268 SGITAVMMEGVTTTLKDIQEEFLKLVFKETILVGHSLENDLLSLKISHNLVIDTAVLYKH 327

Query: 326 PRGASHKVALRILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPSLIR 385
           P G S+K  LRILAKKFL+REIQ+S +GHDS EDA+A M+LALLKI+HGP+FGSPP +IR
Sbjct: 328 PHGRSYKTKLRILAKKFLAREIQESESGHDSAEDAKAAMDLALLKIKHGPDFGSPPEVIR 387

Query: 386 KKLLTVLGESGKMSSFIDDVSTVKRYSSESSHAFPVTSDEEALSRAKKEVMNERVHFVWT 445
           KKLL VL ESGK +S ID+++ VKRY+SESS+A PV+SD+EALS+A KEV N+R  FVWT
Sbjct: 388 KKLLAVLNESGKTTSIIDNINIVKRYASESSNAIPVSSDDEALSKAVKEVKNKRSQFVWT 447

Query: 446 QFSELNSYYKKQADDEERLSVKLAEMLSLLTCDTKSVNRKRNSRKLSAELREILACMDSR 505
           QFSELN++++ +ADD ++L+ +LAEM+SLLTC+ KS  +K N   +S E +EIL  M+ R
Sbjct: 448 QFSELNAHFQSRADDPQKLNSRLAEMISLLTCNKKSGLKKSN---VSPETKEILKKMNER 507

Query: 506 IKNLYTSLPTNAMLIICTGHGDIALVQRLRKMLKEQSETRICREKIVKILEELHAQAETE 565
           +++LY +LPTNAM I+CTGHGD ++V R+RKMLK++ E    REK+VK+LEEL AQAE  
Sbjct: 508 VQSLYAALPTNAMFIVCTGHGDTSIVHRVRKMLKDEDEIGFDREKLVKVLEELQAQAEVA 560

Query: 566 L 567
           L
Sbjct: 568 L 560

BLAST of Sgr028296 vs. TAIR 10
Match: AT3G50100.1 (small RNA degrading nuclease 1 )

HSP 1 Score: 118.2 bits (295), Expect = 2.8e-26
Identity = 67/176 (38.07%), Postives = 102/176 (57.95%), Query Frame = 0

Query: 218 MLALDCEMCVTCEGFE-LTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNGV 277
           M+A+DCEM +  +G E L R+ +VD + +++LD+ VKP+  +VDY T  +GIT E +   
Sbjct: 140 MVAVDCEMVLCEDGTEGLVRVGVVDRDLKVILDEFVKPNKPVVDYRTDITGITAEDIENA 199

Query: 278 TTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKHPRGAS-HKVAL 337
           +  + D Q      +   TILVGHSL  DL  LKI H  V+DTA+++K+P      + +L
Sbjct: 200 SLSVVDIQETLQPFLSTGTILVGHSLNRDLEVLKIDHPKVIDTALVFKYPNTRKLRRPSL 259

Query: 338 RILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPSLIRKKLLTV 392
             L K  L  E++++G  HD + DA A M+LAL  +    +    PS   K++L V
Sbjct: 260 NNLCKSILGYEVRKTGVPHDCVHDASAAMKLALAVVEKRVDTTIKPS---KEMLEV 312

BLAST of Sgr028296 vs. TAIR 10
Match: AT5G05540.1 (small RNA degrading nuclease 2 )

HSP 1 Score: 116.3 bits (290), Expect = 1.1e-25
Identity = 62/173 (35.84%), Postives = 100/173 (57.80%), Query Frame = 0

Query: 217 EMLALDCEMCVTCEGFE-LTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNG 276
           EM+A+DCEM +  +G E + R+  VD + +++LD+ VKP+  +VDY T  +G+T + L  
Sbjct: 141 EMIAIDCEMVLCEDGSEAVVRVAAVDRDLKVILDEFVKPNQPVVDYRTFITGLTAQDLEK 200

Query: 277 VTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKHPRGASH---- 336
            T  + D Q   L  + ++TILVG SL +DL  LK+ H  V+DT++++K+    +     
Sbjct: 201 ATISVVDIQEKLLMFISEDTILVGQSLNHDLKVLKVDHARVIDTSLVFKYNYDGTRRPLR 260

Query: 337 --KVALRILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPS 383
             + +L  L K  L  E+Q+ G  H+ + DA A M+L L  + +G E   P S
Sbjct: 261 LKRPSLNYLCKCILGYEVQKEGVPHNCVHDAEAAMKLVLAILDNGAETSVPLS 313

BLAST of Sgr028296 vs. TAIR 10
Match: AT5G05540.2 (small RNA degrading nuclease 2 )

HSP 1 Score: 116.3 bits (290), Expect = 1.1e-25
Identity = 62/173 (35.84%), Postives = 100/173 (57.80%), Query Frame = 0

Query: 217 EMLALDCEMCVTCEGFE-LTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNG 276
           EM+A+DCEM +  +G E + R+  VD + +++LD+ VKP+  +VDY T  +G+T + L  
Sbjct: 141 EMIAIDCEMVLCEDGSEAVVRVAAVDRDLKVILDEFVKPNQPVVDYRTFITGLTAQDLEK 200

Query: 277 VTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKHPRGASH---- 336
            T  + D Q   L  + ++TILVG SL +DL  LK+ H  V+DT++++K+    +     
Sbjct: 201 ATISVVDIQEKLLMFISEDTILVGQSLNHDLKVLKVDHARVIDTSLVFKYNYDGTRRPLR 260

Query: 337 --KVALRILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPS 383
             + +L  L K  L  E+Q+ G  H+ + DA A M+L L  + +G E   P S
Sbjct: 261 LKRPSLNYLCKCILGYEVQKEGVPHNCVHDAEAAMKLVLAILDNGAETSVPLS 313

BLAST of Sgr028296 vs. TAIR 10
Match: AT5G67240.1 (small RNA degrading nuclease 3 )

HSP 1 Score: 114.0 bits (284), Expect = 5.2e-25
Identity = 67/195 (34.36%), Postives = 102/195 (52.31%), Query Frame = 0

Query: 218 MLALDCEMCVTCE--GFELTRITLVDMEGQIVLDKLVKPSNAIVDYNTRFSGITCEMLNG 277
           ML++DCEM VTCE     L R+  VD + ++VLDK VKP   ++DY T  +G+T E L  
Sbjct: 145 MLSIDCEM-VTCEDGSQALVRVGAVDRDLKVVLDKFVKPDKPVIDYKTDITGVTAEDLER 204

Query: 278 VTTILKDAQRDFLNLVHKETILVGHSLENDLMALKISHDLVVDTAVLYKH-PRGASHKVA 337
            T  + D Q+     +   TILVGH L NDL  L+I H  V+DT+ +++      + + +
Sbjct: 205 ATLSVADIQKKLRRFLSVGTILVGHGLHNDLQVLRIDHARVIDTSYVFEFVDAPKTQRPS 264

Query: 338 LRILAKKFLSREIQQSGNGHDSIEDARATMELALLKIRHGPEFGSPPS----LIRKKLLT 397
           L  L K  L +E++  G  H+ + DA A M+L L  +  G      P+    +  K+   
Sbjct: 265 LNNLCKSVLGQEVRMDGAAHNCVHDAAAAMKLVLAAVEKGAATLIQPTEEMMVAEKRRQE 324

Query: 398 VLGESGKMSSFIDDV 406
              E+GK   F+  +
Sbjct: 325 ARQEAGKAQLFLHKI 338