Sgr028121 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028121 vs. NCBI nr
Match: XP_038898003.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 533.9 bits (1374), Expect = 1.3e-147
Identity = 290/409 (70.90%), Postives = 318/409 (77.75%), Query Frame = 0

Query: 63  LRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLFNKPI 122
           LRPHIQSH YSLSS + RT L TS+TSN  LP S SS S      GF R S L LFN+ I
Sbjct: 11  LRPHIQSHRYSLSSFRRRTSLVTSSTSNNPLPSSLSSSS----PAGFHRESSLRLFNERI 70

Query: 123 WNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVA 182
           W+ N   CSKT+RIDV L  PPLRC ASSNGN V+GERSL QWIELLGE LSTAFPVWVA
Sbjct: 71  WSRNAFFCSKTKRIDVGLLVPPLRCEASSNGNSVQGERSLMQWIELLGEALSTAFPVWVA 130

Query: 183 LGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYS 242
           LGCLLGLLRPAS+ WVQP WTVLGITLTMLGMGMTLTL+ LRGALAMPKELISGFLLQYS
Sbjct: 131 LGCLLGLLRPASYAWVQPGWTVLGITLTMLGMGMTLTLEDLRGALAMPKELISGFLLQYS 190

Query: 243 VMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSIIC- 302
           VMPISGFLVSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+ S +  
Sbjct: 191 VMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTMAA 250

Query: 303 ----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIA 362
                                       +IVLLPVL GAFLNQYFHGL++ VSP +PPIA
Sbjct: 251 VIMTPFLTAKLAGQYVAVDAAGLLMSTLQIVLLPVLVGAFLNQYFHGLVKFVSPFMPPIA 310

Query: 363 VGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTIST 422
           V +V +LCGNAIAQS+SAILMSG+QVVLAA+LLHASGFFFGY+LAR+LGIDV+SSRTIS 
Sbjct: 311 VSTVAILCGNAIAQSSSAILMSGQQVVLAAALLHASGFFFGYVLARLLGIDVASSRTISI 370

Query: 423 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHS+FGS+L GI RQ K
Sbjct: 371 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSVFGSVLAGIWRQSK 415

BLAST of Sgr028121 vs. NCBI nr
Match: XP_022982179.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 522.7 bits (1345), Expect = 3.1e-144
Identity = 286/415 (68.92%), Postives = 319/415 (76.87%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D   LRPHIQ  P SLSS  PRT L TS+TSN      FSS S    A  F R + L +F
Sbjct: 7   DTCVLRPHIQPPPRSLSSFSPRTSLLTSSTSNN----RFSSAS----AATFHRNASLQIF 66

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+RIDV L  PPL+CG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 67  NERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEALSTA 126

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPA +DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 127 FPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 186

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 187 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 246

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 247 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 306

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARML ID+SS
Sbjct: 307 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLSIDISS 366

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. NCBI nr
Match: KAG7037462.1 (putative sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.9 bits (1343), Expect = 5.3e-144
Identity = 287/415 (69.16%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D  GLRPHIQ    SLSS  PRT L TS+T N      FSS S    A  F R + L + 
Sbjct: 24  DTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSAS----AATFHRNASLQIS 83

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+RIDV L  PPLRCG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 84  NQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTA 143

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPAS+DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 144 FPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 203

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 204 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 263

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 264 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 323

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARMLGID SS
Sbjct: 324 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSS 383

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 384 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 430

BLAST of Sgr028121 vs. NCBI nr
Match: XP_022941522.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 521.9 bits (1343), Expect = 5.3e-144
Identity = 287/415 (69.16%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D  GLRPHIQ    SLSS  PRT L TS+T N      FSS S    A  F R + L + 
Sbjct: 7   DTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSAS----AATFHRNASLQIS 66

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+RIDV L  PPLRCG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 67  NQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTA 126

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPAS+DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 127 FPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 186

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 187 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 246

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 247 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 306

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARMLGID SS
Sbjct: 307 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSS 366

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. NCBI nr
Match: KAG6607947.1 (putative sodium/metabolite cotransporter BASS1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.2 bits (1341), Expect = 9.0e-144
Identity = 287/415 (69.16%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D  GLRPHIQ    SLSS  PRT L TS+TSN      FSS S    A  F R + L + 
Sbjct: 7   DTYGLRPHIQPPQRSLSSFSPRTSLLTSSTSNN----RFSSAS----AATFHRNASLQIS 66

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+ IDV L  PPLRCG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 67  NQRISVWNRNPLLCSKTKWIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTA 126

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPAS+DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 127 FPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 186

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 187 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 246

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 247 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 306

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARMLGID SS
Sbjct: 307 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSS 366

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.8e-108
Identity = 204/324 (62.96%), Postives = 246/324 (75.93%), Query Frame = 0

Query: 146 RCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRWTVL 205
           RCG SSN    E ++S  +W+E +GE +STAFP+WV+LGCLLGL+RP++F+WV P WT++
Sbjct: 70  RCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIV 129

Query: 206 GITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAGL 265
           G+T+TMLGMGMTLTLD LRGAL+MPKEL +GFLLQYSVMP+S F VSKLLNLP +YAAGL
Sbjct: 130 GLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGL 189

Query: 266 ILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSII------------------------- 325
           ILVGCCPG   S+           ++ L+T+ S +                         
Sbjct: 190 ILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGL 249

Query: 326 ----CKIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILMSG 385
                ++VLLPVLAGAFLNQYF  L++ VSP++PPIAVG+V +LCG AI Q+ASAILMSG
Sbjct: 250 LMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAILMSG 309

Query: 386 EQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNPLT 441
           +QVVLA+ LLH SGF FGYL +R+LGIDV+SSRTIS EVGMQNSVLGVVLATQHFGNPLT
Sbjct: 310 KQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQNSVLGVVLATQHFGNPLT 369

BLAST of Sgr028121 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 193/337 (57.27%), Postives = 230/337 (68.25%), Query Frame = 0

Query: 143 PPLRCGASSN--------GNGVEGERSLRQWIELL---GEVLSTAFPVWVALGCLLGLLR 202
           P  RC A+++        G     E  +  W +LL   GEVLS  FPVWVA  C + L R
Sbjct: 61  PSSRCHAAADPAPSKIPGGGSGALEAGVVGWRDLLLQVGEVLSLGFPVWVASACAVALWR 120

Query: 203 PASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLV 262
           P +F WV P   ++GI+ TMLGMGMTLTLD L+ AL MPKEL SGFLLQYSVMP+SGFL+
Sbjct: 121 PPAFLWVSPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKELASGFLLQYSVMPLSGFLI 180

Query: 263 SKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSIIC---------- 322
           SKLLNLPSYYAAGLILV CCPG   S+           ++ L+T+ S             
Sbjct: 181 SKLLNLPSYYAAGLILVSCCPGGTASNIVTYLARGNVALSVLMTAASTFAAAFLTPLLTS 240

Query: 323 -------------------KIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCG 382
                              ++VL PVL GA LNQY +GL++LVSPL+P IAV +V VLCG
Sbjct: 241 KLAGQYVAVDPMGLFVSTSQVVLAPVLLGALLNQYCNGLVQLVSPLMPFIAVATVAVLCG 300

Query: 383 NAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVL 440
           NAIAQ+ASAIL SG QVV++   LHASGFFFGY+L+R +GID+SSSRTIS EVGMQNSVL
Sbjct: 301 NAIAQNASAILSSGLQVVMSVCWLHASGFFFGYVLSRTIGIDISSSRTISIEVGMQNSVL 360

BLAST of Sgr028121 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.5e-42
Identity = 112/328 (34.15%), Postives = 164/328 (50.00%), Query Frame = 0

Query: 145 LRCGASSN--GNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRW 204
           L C A +N   N  E   S     E + E+L+T FPVWV LG ++G+ +P+   W++   
Sbjct: 80  LLCKAEANISSNLPESIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSMVTWLETDL 139

Query: 205 TVLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYA 264
             +G+   ML MG+TLT +  R  +  P  +  GFL QY + P+ GF ++  L L +  A
Sbjct: 140 FTVGLGFLMLSMGLTLTFEDFRRCMRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLA 199

Query: 265 AGLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSII---------------------- 324
            GLILV CCPG   S+           ++ L+T+ S I                      
Sbjct: 200 TGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIVMTPLLTKLLAGQLVPVDA 259

Query: 325 -------CKIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAIL 384
                   ++VLLP + G   ++YF      +  + P I V    +LC + I Q +  + 
Sbjct: 260 AGLAISTFQVVLLPTIVGVLAHEYFPKFTERIISITPLIGVLLTTLLCASPIGQVSEVLK 319

Query: 385 MSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGN 442
             G Q+++  +LLH + F  GY L+++     S+SRTIS E GMQ+S LG +LA +HF N
Sbjct: 320 AQGGQLIIPVALLHVAAFALGYWLSKVSSFGESTSRTISIECGMQSSALGFLLAQKHFTN 379

BLAST of Sgr028121 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.9e-41
Identity = 121/378 (32.01%), Postives = 182/378 (48.15%), Query Frame = 0

Query: 85  TSATSNTLLPPSFSSCSSTHLAVGFRRPSPL-PLFNKPIWNWNPLVCSKTQRIDVRLTDP 144
           T+ T   L  P   S S++ +++  +   P+ P+F     +   +VC             
Sbjct: 27  TTRTQTHLDFPKLVSVSNSGISLRIQNSKPISPVFALEATSSRRVVC------------- 86

Query: 145 PLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRWT 204
             +  A  +G+  E         E + E+L+T FP+WV LG L+G+ +P+   W++    
Sbjct: 87  --KAAAGVSGDLPESTPKELSQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWLETDLF 146

Query: 205 VLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAA 264
            LG+   ML MG+TLT +  R  L  P  +  GFL QY + PI GFL++  L L +  A 
Sbjct: 147 TLGLGFLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLAT 206

Query: 265 GLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSII----------------------- 324
           GLILV CCPG   S+           ++ L+T+ S I                       
Sbjct: 207 GLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAA 266

Query: 325 ------CKIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILM 384
                  ++VL+P + G   N++F      +  + P I V    +LC + I Q A  +  
Sbjct: 267 GLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQVADVLKT 326

Query: 385 SGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNP 433
            G Q++L  +LLHA+ F  GY +++      S+SRTIS E GMQ+S LG +LA +HF NP
Sbjct: 327 QGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFLLAQKHFTNP 386

BLAST of Sgr028121 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.1e-30
Identity = 91/298 (30.54%), Postives = 148/298 (49.66%), Query Frame = 0

Query: 166 IELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRG 225
           I  +       F +WV +  +LG   P+ F W+    T+  + + M GMG+TL  D  + 
Sbjct: 4   IRKISHFAGQTFGIWVIVFAVLGFSFPSLFTWISSYITIF-LGIIMFGMGLTLQADDFKE 63

Query: 226 ALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRL---- 285
            +  P ++I G + QY++MP+  F ++  L+LP+  A G+ILVGCCPG   S+       
Sbjct: 64  LVRKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNVMTFLAK 123

Query: 286 GYRHFFHLITTLVTSLS-------------------------IICKIVLLPVLAGAFLNQ 345
           G       +TT+ T L+                          I + VL P++AG  +  
Sbjct: 124 GNTALSVAVTTISTLLAPVVTPLLIMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKM 183

Query: 346 YFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYL 405
           +F   +      +P ++V  +  +    ++ +   +L SG  +     L +  G+  G+L
Sbjct: 184 FFRKQVAKAVHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLIFSVVILHNGIGYLLGFL 243

Query: 406 LARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSIL 435
            A++L +D  S + I+ EVGMQNS LG  LAT HF +PL+AVP A+ SV H++ GS+L
Sbjct: 244 CAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHF-SPLSAVPSAIFSVWHNLSGSML 299

BLAST of Sgr028121 vs. ExPASy TrEMBL
Match: A0A6J1J3V0 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481085 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.5e-144
Identity = 286/415 (68.92%), Postives = 319/415 (76.87%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D   LRPHIQ  P SLSS  PRT L TS+TSN      FSS S    A  F R + L +F
Sbjct: 7   DTCVLRPHIQPPPRSLSSFSPRTSLLTSSTSNN----RFSSAS----AATFHRNASLQIF 66

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+RIDV L  PPL+CG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 67  NERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEALSTA 126

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPA +DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 127 FPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 186

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 187 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 246

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 247 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 306

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARML ID+SS
Sbjct: 307 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLSIDISS 366

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. ExPASy TrEMBL
Match: A0A6J1FMN4 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111446800 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.5e-144
Identity = 287/415 (69.16%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D  GLRPHIQ    SLSS  PRT L TS+T N      FSS S    A  F R + L + 
Sbjct: 7   DTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSAS----AATFHRNASLQIS 66

Query: 119 NK--PIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTA 178
           N+   +WN NPL+CSKT+RIDV L  PPLRCG+SS+ NGV+ ERSL QWIEL+GE LSTA
Sbjct: 67  NQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTA 126

Query: 179 FPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISG 238
           FPVWVALGCLLGLLRPAS+DWVQPRWTVLG+T+TMLGMGMTLTLD LRGALAMPKELISG
Sbjct: 127 FPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISG 186

Query: 239 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTS 298
           FLLQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+
Sbjct: 187 FLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 246

Query: 299 LSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSP 358
           +S +                              +IVLLPVLAGAFLNQYFHGL+R VSP
Sbjct: 247 MSTVAAVIMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSP 306

Query: 359 LIPPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSS 418
           L+PPIAVG+V VLCGNAIAQS+SAIL SG QV+LAASLLH SGFFFGY+LARMLGID SS
Sbjct: 307 LMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSS 366

Query: 419 SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           SRTIS EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 SRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. ExPASy TrEMBL
Match: A0A6J1BV26 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005764 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 9.7e-144
Identity = 292/405 (72.10%), Postives = 319/405 (78.77%), Query Frame = 0

Query: 69  SHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLFNKPIWNWNPL 128
           S PYSL S   R  L  S+TS   LP S SS S      GFR  S L L ++ I  WNP+
Sbjct: 6   SLPYSLPSCSYRPFL-VSSTSGNFLPSSLSSSS----PAGFRPASSLRLSDERIRVWNPV 65

Query: 129 VCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLG 188
             S+ +RIDVRL  PP RCG SS+G+GVEGERSLRQWIELLGE LSTAFPVWVALGCLLG
Sbjct: 66  FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG 125

Query: 189 LLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISG 248
           LLRPA+F+WV+PRWTVLGITLTMLGMGMTLTLD LRGALAMPKELISGFLLQYSVMPISG
Sbjct: 126 LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG 185

Query: 249 FLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHL-ITTLVTSLSIIC------ 308
           FLVSKLLNLPSYYAAGLILVGCCPG   +SN + Y    ++ ++ L+T+ S +       
Sbjct: 186 FLVSKLLNLPSYYAAGLILVGCCPGG-TASNIVAYIARGNVALSVLMTAASTVAAVIMTP 245

Query: 309 -----------------------KIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVG 368
                                  +IVLLPVLAGAFLNQYFHGL+RLVSPL+PPIAVG+V 
Sbjct: 246 FLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVA 305

Query: 369 VLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQ 428
           VLCGNAIAQS+SAILMSG+QVVLAASLLHASGFFFGY+LARMLGIDVSSSRTIS EVGMQ
Sbjct: 306 VLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQ 365

Query: 429 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRKG 444
           NSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSIL GI RQ KG
Sbjct: 366 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAGIWRQSKG 404

BLAST of Sgr028121 vs. ExPASy TrEMBL
Match: A0A0A0KZF9 (Sodium-bile acid cotransporter OS=Cucumis sativus OX=3659 GN=Csa_4G622840 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.8e-142
Identity = 280/413 (67.80%), Postives = 317/413 (76.76%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D   LRP    + YSLS+ +PRT L TS++S+  +P   +S  S+   VG RR SPL LF
Sbjct: 7   DTCVLRP----YRYSLSTFRPRTSLVTSSSSSFNIP--LNSSLSSFSLVGLRRESPLLLF 66

Query: 119 NKPIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFP 178
             PIWN NP VCS+T+R D  L  PPLRC  SSNGN V+GERSL QWIEL+GE LSTAFP
Sbjct: 67  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 126

Query: 179 VWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISGFL 238
           VWVALGC+LGLLRPAS+ WVQPRWTVLGITLTMLGMGMTLTLD LRGALAMPKELISGF+
Sbjct: 127 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 186

Query: 239 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLS 298
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+ S
Sbjct: 187 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTATS 246

Query: 299 IIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSPLI 358
            +                              +IVLLPVL GAFLNQYFHGL+R VSP +
Sbjct: 247 TMAAVIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAFLNQYFHGLVRFVSPFM 306

Query: 359 PPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSR 418
           PPIAVG+V +LCG+AIAQS+SAI MSG+QVVLAA+LLHASGFFFGY+LAR+LGID++SSR
Sbjct: 307 PPIAVGTVAILCGHAIAQSSSAIRMSGQQVVLAAALLHASGFFFGYVLARLLGIDIASSR 366

Query: 419 TISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           TIS EVGMQNSVLGVVLA+QHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 TISIEVGMQNSVLGVVLASQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of Sgr028121 vs. ExPASy TrEMBL
Match: A0A5D3DX81 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00270 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.9e-140
Identity = 280/413 (67.80%), Postives = 313/413 (75.79%), Query Frame = 0

Query: 59  DKAGLRPHIQSHPYSLSSSKPRTLLFTSATSNTLLPPSFSSCSSTHLAVGFRRPSPLPLF 118
           D   LRP    + YSLS+  PRT L TS++SN  L  S SS S     VG RR S L LF
Sbjct: 7   DTCVLRP----YRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFS----LVGLRRESSLLLF 66

Query: 119 NKPIWNWNPLVCSKTQRIDVRLTDPPLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFP 178
              IWN NP VCS+T+R D  L  PPLRC  SSNGN ++GERSL QWIEL+GE LSTAFP
Sbjct: 67  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 126

Query: 179 VWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKELISGFL 238
           VWVALGC+LGLLRPAS+ WVQPRWTVLGITLTMLGMGMTLTLD LRGALAMPKELISGF+
Sbjct: 127 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 186

Query: 239 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLS 298
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPG   S+           ++ L+T+ S
Sbjct: 187 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTATS 246

Query: 299 IIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRLVSPLI 358
            +                              +IVLLPVL GAFLNQYFHGL+R VSP +
Sbjct: 247 TMAAVIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAFLNQYFHGLVRFVSPFM 306

Query: 359 PPIAVGSVGVLCGNAIAQSASAILMSGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSR 418
           PPIAVG+V VLCG+AIAQS+SAI +SG+QVVLAA+LLHASGFFFGY+LAR+LGID++SSR
Sbjct: 307 PPIAVGTVAVLCGHAIAQSSSAIRISGQQVVLAAALLHASGFFFGYVLARLLGIDIASSR 366

Query: 419 TISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILTGILRQRK 443
           TIS EVGMQNSVLGVVLA+QHFGNPLTAVPCAVSSVCHSIFGS+L GI RQ K
Sbjct: 367 TISIEVGMQNSVLGVVLASQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 411

BLAST of Sgr028121 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 392.9 bits (1008), Expect = 3.4e-109
Identity = 204/324 (62.96%), Postives = 246/324 (75.93%), Query Frame = 0

Query: 146 RCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRWTVL 205
           RCG SSN    E ++S  +W+E +GE +STAFP+WV+LGCLLGL+RP++F+WV P WT++
Sbjct: 70  RCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIV 129

Query: 206 GITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAGL 265
           G+T+TMLGMGMTLTLD LRGAL+MPKEL +GFLLQYSVMP+S F VSKLLNLP +YAAGL
Sbjct: 130 GLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGL 189

Query: 266 ILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSII------------------------- 325
           ILVGCCPG   S+           ++ L+T+ S +                         
Sbjct: 190 ILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGL 249

Query: 326 ----CKIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILMSG 385
                ++VLLPVLAGAFLNQYF  L++ VSP++PPIAVG+V +LCG AI Q+ASAILMSG
Sbjct: 250 LMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAILMSG 309

Query: 386 EQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNPLT 441
           +QVVLA+ LLH SGF FGYL +R+LGIDV+SSRTIS EVGMQNSVLGVVLATQHFGNPLT
Sbjct: 310 KQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQNSVLGVVLATQHFGNPLT 369

BLAST of Sgr028121 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 170.6 bits (431), Expect = 2.8e-42
Identity = 121/378 (32.01%), Postives = 182/378 (48.15%), Query Frame = 0

Query: 85  TSATSNTLLPPSFSSCSSTHLAVGFRRPSPL-PLFNKPIWNWNPLVCSKTQRIDVRLTDP 144
           T+ T   L  P   S S++ +++  +   P+ P+F     +   +VC             
Sbjct: 27  TTRTQTHLDFPKLVSVSNSGISLRIQNSKPISPVFALEATSSRRVVC------------- 86

Query: 145 PLRCGASSNGNGVEGERSLRQWIELLGEVLSTAFPVWVALGCLLGLLRPASFDWVQPRWT 204
             +  A  +G+  E         E + E+L+T FP+WV LG L+G+ +P+   W++    
Sbjct: 87  --KAAAGVSGDLPESTPKELSQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWLETDLF 146

Query: 205 VLGITLTMLGMGMTLTLDALRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAA 264
            LG+   ML MG+TLT +  R  L  P  +  GFL QY + PI GFL++  L L +  A 
Sbjct: 147 TLGLGFLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLAT 206

Query: 265 GLILVGCCPGSDISSNRLGYRHFFHLITTLVTSLSII----------------------- 324
           GLILV CCPG   S+           ++ L+T+ S I                       
Sbjct: 207 GLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAA 266

Query: 325 ------CKIVLLPVLAGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILM 384
                  ++VL+P + G   N++F      +  + P I V    +LC + I Q A  +  
Sbjct: 267 GLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQVADVLKT 326

Query: 385 SGEQVVLAASLLHASGFFFGYLLARMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNP 433
            G Q++L  +LLHA+ F  GY +++      S+SRTIS E GMQ+S LG +LA +HF NP
Sbjct: 327 QGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFLLAQKHFTNP 386

BLAST of Sgr028121 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-24
Identity = 92/318 (28.93%), Postives = 143/318 (44.97%), Query Frame = 0

Query: 171 EVLSTAF---PVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGAL 230
           E L  A+   P  + L  +L L+ P SF W +PR+ V G+   M  +G+         AL
Sbjct: 93  ETLKQAYSFIPHGILLSTILALVYPPSFTWFKPRYFVPGLGFMMFAVGINSNERDFLEAL 152

Query: 231 AMPKELISGFLLQYSVMPISGFLVS----KLLNLPSYYAAGLILVGCCPGSDISSNRLGY 290
             P  + +G++ QY + P+ G++       L NLP+   AG++LV C  G+ +S+    Y
Sbjct: 153 KRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSGAQLSN----Y 212

Query: 291 RHFFH--------------------LITTLVTSL--------------SIICKIVLLPVL 350
             F                      L+T +++ L              S I ++V+ P+ 
Sbjct: 213 TTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQVVITPIA 272

Query: 351 AGAFLNQYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILMS-GEQVVLAASLLHA 410
           AG  LN+ F  L   + P +P + V  +    G  +A +  +IL   G  ++      H 
Sbjct: 273 AGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLALNIDSILSPFGATILFLVITFHL 332

Query: 411 SGFFFGYLLARML---GIDVSS-SRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSS 443
             F  GY           DV +  RTIS E GMQ+S+L + LAT+ F +PL  VP A+S+
Sbjct: 333 LAFVAGYFFTGFFFSKAPDVKALQRTISYETGMQSSLLALALATKFFQDPLVGVPPAIST 392

BLAST of Sgr028121 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 103.6 bits (257), Expect = 4.2e-22
Identity = 84/312 (26.92%), Postives = 141/312 (45.19%), Query Frame = 0

Query: 174 STAFPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKEL 233
           ++  P  V    +L L+ P SF W   R+ V  +   M  +G+         A   PK +
Sbjct: 98  NSILPHVVLASTILALIYPPSFTWFTSRYFVPALGFLMFAVGINSNEKDFLEAFKRPKAI 157

Query: 234 ISGFLLQYSVMPISGFLVS----KLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFH- 293
           + G++ QY V P+ GF+       L  LP+   AG++LV C  G+ +S+    Y  F   
Sbjct: 158 LLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQLSN----YATFLTD 217

Query: 294 --------LITTLVTS-------------------------LSIICKIVLLPVLAGAFLN 353
                   ++T+L T+                         +S I ++V+ P+ AG  LN
Sbjct: 218 PALAPLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVIAPIAAGLLLN 277

Query: 354 QYFHGLLRLVSPLIPPIAVGSVGVLCGNAIAQSASAILMS-GEQVVLAASLLHASGFFFG 413
           + F  +   + P +P ++V       G  +A + ++++   G  ++L  ++ H S F  G
Sbjct: 278 KLFPKVSNAIRPFLPILSVLDTACCVGAPLALNINSVMSPFGATILLLVTMFHLSAFLAG 337

Query: 414 YLLA----RMLGIDVSSSRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIF 443
           Y L     R      +  RT+S E GMQ+S+L + LAT+ F +PL  +P A+S+V  S+ 
Sbjct: 338 YFLTGSVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFFQDPLVGIPPAISTVVMSLM 397

BLAST of Sgr028121 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 97.4 bits (241), Expect = 3.0e-20
Identity = 85/293 (29.01%), Postives = 133/293 (45.39%), Query Frame = 0

Query: 174 STAFPVWVALGCLLGLLRPASFDWVQPRWTVLGITLTMLGMGMTLTLDALRGALAMPKEL 233
           S   P  VAL  +  L  P SF WV        +   ML +G+ L++D    A   P  L
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPL 171

Query: 234 ISGFLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGSDISSNRLGYRHFFHLITTL 293
             GF+ QY + P+ G LV+    +P  + AG IL  C  G+ +SS           ++ L
Sbjct: 172 SVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSIL 231

Query: 294 VTSLSIIC-----------------------------KIVLLPVLAGAFLNQYFHGLLRL 353
           +TS + I                              ++VL+P+  G  LN Y   ++ L
Sbjct: 232 LTSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVVTL 291

Query: 354 VSPLIPPIAVGSVGVLCGNAIAQSASAILMS-GEQVVLAASLLHASGFFFGYLLARMLGI 413
           + P++P +A+    +  G+ ++ + S IL + G  +++     HA  F  GY  +++ G+
Sbjct: 292 LQPVMPFVAMVCTSLCIGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIPGL 351

Query: 414 --DVSSSRTISTEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSIL 435
             +   SRTIS   GMQ+S L  +LA+Q  G+   AVP A S V  +I G  L
Sbjct: 352 RQEEEVSRTISLCTGMQSSTLAGLLASQFLGSS-QAVPAACSVVVMAIMGLCL 403