Sgr028084 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028084 vs. NCBI nr
Match: XP_022159075.1 (vacuolar protein sorting-associated protein 53 A [Momordica charantia] >XP_022159460.1 vacuolar protein sorting-associated protein 53 A [Momordica charantia] >XP_022159541.1 vacuolar protein sorting-associated protein 53 A [Momordica charantia] >XP_022159620.1 vacuolar protein sorting-associated protein 53 A [Momordica charantia])

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 769/891 (86.31%), Postives = 783/891 (87.88%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQ+MASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQLMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
           GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV
Sbjct: 301 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQGTENDEKDG KDVSVPGAGFNFRGI+SSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGTENDEKDGVKDVSVPGAGFNFRGIISSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DI+EGSQNNVLSSSMQ                                  +F ++     
Sbjct: 421 DIEEGSQNNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTLFNLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLFNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ANKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVDGIDMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDGIDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILTSSIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTSSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPA 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGI QPSVSPSA  + ++TPPAPTISSPA
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGIMQPSVSPSAPHIVTSTPPAPTISSPA 822

Query: 925 SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           SVG+MASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 SVGVMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 822

BLAST of Sgr028084 vs. NCBI nr
Match: XP_004149523.1 (vacuolar protein sorting-associated protein 53 A [Cucumis sativus] >KGN55451.2 hypothetical protein Csa_012355 [Cucumis sativus])

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 762/892 (85.43%), Postives = 781/892 (87.56%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQS+SGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSNSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGARGKE+GN IEEFGRED+NSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG ENDEK+G KD+SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPENDEKNGIKDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSP+DSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPVDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV-SPSAAPVASTTPPAPTISSP 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV SPSA PV S+TPPAPTI+SP
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSSPSAPPVVSSTPPAPTITSP 823

Query: 925 ASVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           ++VGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 STVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Sgr028084 vs. NCBI nr
Match: XP_008464655.1 (PREDICTED: vacuolar protein sorting-associated protein 53 A isoform X1 [Cucumis melo])

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 761/892 (85.31%), Postives = 780/892 (87.44%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGARGKE+GN IEEFGRED+NSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG ENDEK+G KD+SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPENDEKNGIKDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPV GRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVFGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSP+DSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPVDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV-SPSAAPVASTTPPAPTISSP 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV SPSA PV S+TPPAPT++SP
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSP 823

Query: 925 ASVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           ++VGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 STVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Sgr028084 vs. NCBI nr
Match: XP_038897182.1 (vacuolar protein sorting-associated protein 53 A [Benincasa hispida] >XP_038897183.1 vacuolar protein sorting-associated protein 53 A [Benincasa hispida])

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 761/892 (85.31%), Postives = 779/892 (87.33%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGARGKE+GNEIEEFGRED+NSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGARGKESGNEIEEFGREDSNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG ENDEK+G KD+ VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPENDEKNGIKDMPVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ANKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVD +DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDAVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGA SYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGATSYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV-SPSAAPVASTTPPAPTISSP 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV SPSA PV S+TPPAPT++SP
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSP 823

Query: 925 ASVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           ++VGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 STVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Sgr028084 vs. NCBI nr
Match: XP_023535153.1 (vacuolar protein sorting-associated protein 53 A [Cucurbita pepo subsp. pepo] >KAG6591821.1 Vacuolar protein sorting-associated protein 53 A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024687.1 Vacuolar protein sorting-associated protein 53 A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 758/891 (85.07%), Postives = 776/891 (87.09%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRR+RTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRMRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGA+GKEN NEIEEFGREDNNSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG E DEKDG K++SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPETDEKDGIKEMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KL ARLPKGG+GFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLLARLPKGGSGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           ID+QLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDTQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPA 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPS  P+AS  PPAPTI+SP 
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSVPPIASAAPPAPTITSP- 821

Query: 925 SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           +VGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 TVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Sgr028084 vs. ExPASy Swiss-Prot
Match: Q0WQF4 (Vacuolar protein sorting-associated protein 53 A OS=Arabidopsis thaliana OX=3702 GN=VPS53 PE=1 SV=1)

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 653/897 (72.80%), Postives = 714/897 (79.60%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASL+GVEPLMQKI  EIRRVDA IL+AVRQQS+SGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
            AT AVEEL  KI+EIK+KAEQSE MVQEICRDIKKLDFAKK+ITTTITALHRLTMLVSA
Sbjct: 61  DATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLC+HFEAYRD PKITELREK  NIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKE EETNLLQ+LSD+C VVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFP SWHVPYRLCIQFCK+TRKQ+E IL N+KEKP V
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
             LLLALQ T+EFE EL +KFGGG   K+  ++IEE G  ++NSQN+S IRKKYEKK A 
Sbjct: 301 AILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAA 360

Query: 445 HQGTEND----EKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQ 504
            Q TE +    EK G KD+SV GAGFNFRG++SSCFEPHLT YIELEEKTLM++LEK+VQ
Sbjct: 361 SQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQ 420

Query: 505 EETWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLV 564
           EE+WD+++GSQNNVLSSS Q                                        
Sbjct: 421 EESWDVEDGSQNNVLSSSTQ---------------------------------------- 480

Query: 565 QRMREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRV 624
                                        + SN +K LK    L KNQTLFNLFKVFQRV
Sbjct: 481 -----------------------------LFSNIKKSLKRCNTLSKNQTLFNLFKVFQRV 540

Query: 625 LKAYAIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAES 684
           LKAYA KLF +LPKGGTG VAAATGMDGQIK S++DE+VICYIVNSAEYCHKTSGELAE+
Sbjct: 541 LKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAEN 600

Query: 685 VQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVG 744
           V +IID    DG+DMSEVQDEFSA+ITKALVTLV GLETKFD+EMA MTRVPW TLESVG
Sbjct: 601 VSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVG 660

Query: 745 DQSEYVNGINMILTSSIPVLGRLLSPLYFQFFLDK------------------------- 804
           DQS YVNGIN +L+ SIPVLG+LL+P+YFQFFLDK                         
Sbjct: 661 DQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQ 720

Query: 805 -MLLDTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRA 864
            MLLDTQAVK+ILL+IPSL RQTS AASYSKFVSREMS+AEALLKVILSPIDSVADTYRA
Sbjct: 721 QMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRA 780

Query: 865 LLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAP-- 924
           L PEGTPMEFQRILELKG KKADQQSILDDFNKHGPG TQ SV+ +A P    TPPAP  
Sbjct: 781 LFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVA-AAMPQPMPTPPAPPL 827

Query: 925 TISSPA-SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFN 949
            I++PA + G +A+ EDVLTRAAALGRGAA+TGFK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 841 AITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 827

BLAST of Sgr028084 vs. ExPASy Swiss-Prot
Match: F4I7Y2 (Vacuolar protein sorting-associated protein 53 B OS=Arabidopsis thaliana OX=3702 GN=At1g50970 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.6e-151
Identity = 384/873 (43.99%), Postives = 439/873 (50.29%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSS L++INQMFP EASLS VE  MQKI +EIRRVDA ILA V QQ +SGT+AKE+L 
Sbjct: 1   MDKSSGLDFINQMFPVEASLSCVESFMQKICDEIRRVDATILAVVSQQGNSGTRAKENLN 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
            A  A EEL  KI+EIK+KAEQ+E MVQ+IC DIKKLDFAKK+ITT +TAL RLTMLVSA
Sbjct: 61  DAICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           V+QLQVM SKRQYKEAA QLEA+N+LC+HF+AY D PKI ELREK KNIKQILK HVFSD
Sbjct: 121 VQQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQILKFHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTG E EE  LL++LSD+C VVDALEPSVREEL+NNFCSRELTSYEQI+ GAEL  
Sbjct: 181 FSSLGTGTETEELFLLKKLSDSCLVVDALEPSVREELINNFCSRELTSYEQIYVGAELKT 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LD+ E  Y  +   IR N+  W IFP SWHVPYRLCIQ  +KTR Q+E IL NLKEK DV
Sbjct: 241 LDEIELIYNQLSCLIRKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
             LLL L+RTLEFE EL  KFGGG      G++I   G   NNSQ               
Sbjct: 301 EKLLLELKRTLEFERELEMKFGGGG---SIGDDI--IGGGGNNSQK-------------- 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
                                FNFRG++SSCFEPHLT+YIE EE  LM+ LEK+VQEETW
Sbjct: 361 ---------------------FNFRGMISSCFEPHLTIYIEKEEMELMQLLEKVVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DI+E                                                        
Sbjct: 421 DIEE-------------------------------------------------------- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                              E+ C  +N                 
Sbjct: 481 -----------------------------------ELGCHSEN----------------- 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
                                                                       
Sbjct: 541 ------------------------------------------------------------ 569

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
                                                                     S 
Sbjct: 601 ----------------------------------------------------------SV 569

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDKMLLDTQAVKTILLDIPSLGRQ-------- 804
           Y+                             MLLD   +K ILL +PSL RQ        
Sbjct: 661 YL-----------------------------MLLDAHDMKMILLKVPSLARQPEASALLV 569

Query: 805 TSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKA 864
            +  ASY K V+ +M +AEA+LKVI SPI +V DTYRAL PE TPMEFQRIL LKG  KA
Sbjct: 721 KTATASYVKLVNHQMKRAEAVLKVIASPIVTVIDTYRALFPEETPMEFQRILVLKGLTKA 569

Query: 865 DQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPASVGLMASREDVLTRAAAL 924
           +QQSILDDFN H   ITQ SV+ +  P A   P A T  +PA V   A+ E+VLTRAA+ 
Sbjct: 781 EQQSILDDFNNHSSRITQLSVA-AKTPEAHALPLALTNVAPA-VRFKANSEEVLTRAAS- 569

Query: 925 GRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
              AATT F +  ALT AAKDR   PFRKLFNP
Sbjct: 841 ---AATTSFMKLYALTGAAKDR---PFRKLFNP 569

BLAST of Sgr028084 vs. ExPASy Swiss-Prot
Match: Q5ZLD7 (Vacuolar protein sorting-associated protein 53 homolog OS=Gallus gallus OX=9031 GN=VPS53 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 7.7e-95
Identity = 259/854 (30.33%), Postives = 432/854 (50.59%), Query Frame = 0

Query: 89  SALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATS 148
           +A+EYIN +FPTE SL+ ++ ++ KI  +IR++D  I   VR Q++ G   ++ L  A  
Sbjct: 42  NAVEYINTLFPTEQSLANIDEVVNKIRLKIRKLDDNIRTVVRGQTNVGQDGRQALEEAQK 101

Query: 149 AVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQL 208
           A+++L  KI++IK KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L
Sbjct: 102 AIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSL 161

Query: 209 QVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS-- 268
           + M  +RQY E A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF   
Sbjct: 162 EAMTRRRQYGEVANLLQGVVNVLEHFNKYMGIPQIRQLSERVKAAQNELGQQILADFEEA 221

Query: 269 --SLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELA 328
             S GT +    +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A
Sbjct: 222 FPSQGTKRPGGPSNVLR---DACLVANVLDPRIKQEIIKKFIKQHLSEYLVLFQENQDVA 281

Query: 329 KLDKTERRYAWIKRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKP 388
            LDK +RRYAWIKR++   EE + ++FP  W +  R+ ++FC  TR +L  I+    ++ 
Sbjct: 282 WLDKIDRRYAWIKRQLVDYEEKYGRMFPQEWCMTERIAVEFCHVTRTELAKIMRTRAKEI 341

Query: 389 DVGTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKL 448
           +V  LL A+QRT  FE  LA++F G      +G   +      + +  + D       ++
Sbjct: 342 EVKLLLFAIQRTTNFEGLLAKRFSGCTLA--DGTVKKPEAPPPSTNPFLEDETGTETDEI 401

Query: 449 AVHQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQE- 508
            +    E  + D  K   VP   F+  GIVS CFEPHL VYIE ++K L E +++ V + 
Sbjct: 402 VI----EKSDADKPKKPKVPDNPFH--GIVSKCFEPHLYVYIESQDKNLSELIDRFVADF 461

Query: 509 -----ETWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIF 568
                   ++DEG    VL S          +YY                          
Sbjct: 462 KAQGPPKPNVDEGGA--VLPSCADLF-----VYY-------------------------- 521

Query: 569 QVLVQRMREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKV 628
                                                 +K + +   L   + +  L  +
Sbjct: 522 --------------------------------------KKCMVQCSQLSTGEPMIALTTI 581

Query: 629 FQRVLKAYAIKLFA-RLPK-----GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAE 688
           FQ+ L+ YA K+ +  LPK     GG    +     +G    K + ++  +IC I+++AE
Sbjct: 582 FQKYLREYAWKILSGNLPKTTSSSGGLTITSLLKEKEGSEVAKFTLEELCLICSILSTAE 641

Query: 689 YCHKTSGELAESVQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAM 748
           YC  T+ +L E +++ +D+ LV+ I+++   D FS +I+ ++  LV  L+   D  + AM
Sbjct: 642 YCLATTQQLEEKLKEKVDASLVERINLTGETDTFSIVISNSIQLLVQDLDAACDPALTAM 701

Query: 749 TRVPWGTLESVGDQSEYVNGINMILTSSIPVLGRLLSPL--YFQFF-------------- 808
           +++ W  +E VGDQS YV  + + +  ++P++   L+    YF  F              
Sbjct: 702 SKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFIN 761

Query: 809 ------------LDKMLLDTQAVKTILLDIPSLGRQT--SGAASYSKFVSREMSKAEALL 868
                        +++LLDT ++K +LLD+PS+G Q      ASY++ V + M++AE +L
Sbjct: 762 HLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTRIVVKGMTRAEMIL 813

Query: 869 KVILSPIDS---VADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQP 890
           KV+++P +      D Y  LL + +   FQ+IL++KG K+++Q S+L+ F +  P     
Sbjct: 822 KVVMAPHEPPVVFVDNYIKLLADCSTDTFQKILDMKGLKRSEQSSMLELFRQRLPAPPSG 813

BLAST of Sgr028084 vs. ExPASy Swiss-Prot
Match: Q5R5J4 (Vacuolar protein sorting-associated protein 53 homolog OS=Pongo abelii OX=9601 GN=VPS53 PE=2 SV=2)

HSP 1 Score: 349.0 bits (894), Expect = 1.7e-94
Identity = 261/848 (30.78%), Postives = 432/848 (50.94%), Query Frame = 0

Query: 89  SALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATS 148
           +A+EYIN +FPTE SL+ ++ ++ KI  +IRR+D  I   VR Q++ G   ++ L  A  
Sbjct: 43  NAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQK 102

Query: 149 AVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQL 208
           A+++L  KI++IK KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L
Sbjct: 103 AIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSL 162

Query: 209 QVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS-- 268
           + M  +RQY E A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF   
Sbjct: 163 EAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEA 222

Query: 269 --SLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELA 328
             S GT +    +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A
Sbjct: 223 FPSQGTKRPGGPSNVLR---DACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVA 282

Query: 329 KLDKTERRYAWIKRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKP 388
            LDK +RRYAWIKR++   EE + ++FP  W +  R+ ++FC  TR +L  I+    ++ 
Sbjct: 283 WLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRTELAKIMRTRAKEI 342

Query: 389 DVGTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKL 448
           +V  LL A+QRT  FE  LA++F G         ++E      + +  + D      ++L
Sbjct: 343 EVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLE--SPPPSTNPFLEDEPTPEMEEL 402

Query: 449 AVHQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEE 508
           A    TE  + D  K    P   F+  GIVS CFEPHL VYIE ++K L E +++ V   
Sbjct: 403 A----TEKGDLDQPKKPKAPDNPFH--GIVSKCFEPHLYVYIESQDKNLGELIDRFV--- 462

Query: 509 TWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQR 568
               D  +Q     ++ +    GG +                        PS   + V  
Sbjct: 463 ---ADFKAQGPPKPNTDE----GGAVL-----------------------PSCADLFVY- 522

Query: 569 MREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLK 628
                                           +K + +   L   + +  L  +FQ+ L+
Sbjct: 523 -------------------------------YKKCMVQCSQLSTGEPMIALTTIFQKYLR 582

Query: 629 AYAIKLFA-RLPK-----GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAEYCHKTS 688
            YA K+ +  LPK     GG    +     +G    K + ++  +IC I+++AEYC  T+
Sbjct: 583 EYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATT 642

Query: 689 GELAESVQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWG 748
            +L E +++ +D  L++ I+++   D FS +I+ ++  LV  L+   D  + AM+++ W 
Sbjct: 643 QQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQ 702

Query: 749 TLESVGDQSEYVNGINMILTSSIPVLGRLLSPL--YFQFF-------------------- 808
            +E VGDQS YV  + + +  ++P++   L+    YF  F                    
Sbjct: 703 NVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCK 762

Query: 809 ------LDKMLLDTQAVKTILLDIPSLGRQT--SGAASYSKFVSREMSKAEALLKVILSP 868
                  +++LLDT ++K +LLD+PS+G Q      ASY+K V + M++AE +LKV+++P
Sbjct: 763 PISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAP 814

Query: 869 IDSV---ADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSA 890
            + +    D Y  LL +     FQ+IL++KG K+++Q S+L+   +  P     + S  +
Sbjct: 823 HEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGS 814

BLAST of Sgr028084 vs. ExPASy Swiss-Prot
Match: Q8CCB4 (Vacuolar protein sorting-associated protein 53 homolog OS=Mus musculus OX=10090 GN=Vps53 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.9e-94
Identity = 260/849 (30.62%), Postives = 431/849 (50.77%), Query Frame = 0

Query: 89  SALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATS 148
           +A+EYIN +FPTE SL+ ++ ++ KI  +IRR+D  I   VR Q++ G   ++ L  A  
Sbjct: 43  NAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQK 102

Query: 149 AVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQL 208
           A+++L  KI++IK KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L
Sbjct: 103 AIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSL 162

Query: 209 QVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS-- 268
           + M  +RQY E A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF   
Sbjct: 163 EAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEA 222

Query: 269 --SLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELA 328
             S GT +    +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A
Sbjct: 223 FPSQGTKRPGGPSNVLR---DACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVA 282

Query: 329 KLDKTERRYAWIKRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKP 388
            LDK +RRYAW+KR++   EE + ++FP  W +  R+ ++FC  TR +L  I+    ++ 
Sbjct: 283 WLDKIDRRYAWVKRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEI 342

Query: 389 DVGTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKL 448
           +V  LL A+QRT  FE  LA++F G         ++E      + +  + D      ++L
Sbjct: 343 EVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLE--SPPPSTNPFLEDETTPEMEEL 402

Query: 449 AVHQGTENDEKDGTKDVSVPGAGFN-FRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQE 508
           A+ +G     K        P A  N F GIVS CFEPHL VYIE ++K L E +++ V  
Sbjct: 403 ALEKGELEQPKK-------PKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFV-- 462

Query: 509 ETWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQ 568
                D  +Q     ++ +    GG +                        PS   + V 
Sbjct: 463 ----ADFKAQGPPKPNTDE----GGAVL-----------------------PSCADLFVY 522

Query: 569 RMREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVL 628
                                            +K + +   L   + +  L  +FQ+ L
Sbjct: 523 --------------------------------YKKCMVQCSQLSTGEPMIALTTIFQKYL 582

Query: 629 KAYAIKLFA-RLPK-----GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAEYCHKT 688
           + YA K+ +  LPK     GG    +     +G    + + ++  +IC I+++AEYC  T
Sbjct: 583 REYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLAT 642

Query: 689 SGELAESVQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPW 748
           + +L E +++ +D  L + I+++   D FS +I+ ++  LV  L+   D  + AM+++PW
Sbjct: 643 TQQLEEKLKEKVDVSLTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPW 702

Query: 749 GTLESVGDQSEYVNGINMILTSSIPVLGRLLSPL--YFQFF------------------- 808
             +E VGDQS YV  + + +  ++P++   L+    YF  F                   
Sbjct: 703 QNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKC 762

Query: 809 -------LDKMLLDTQAVKTILLDIPSLGRQT--SGAASYSKFVSREMSKAEALLKVILS 868
                   +++LLDT ++K +LLD+PS+G Q      ASY+K V + M++AE +LKV+++
Sbjct: 763 KPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMA 814

Query: 869 PIDSV---ADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPS 890
           P + +    D Y  LL +     FQ+IL++KG K+++Q S+L+   +  P     +   S
Sbjct: 823 PHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSS 814

BLAST of Sgr028084 vs. ExPASy TrEMBL
Match: A0A6J1DXM1 (vacuolar protein sorting-associated protein 53 A OS=Momordica charantia OX=3673 GN=LOC111025277 PE=3 SV=1)

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 769/891 (86.31%), Postives = 783/891 (87.88%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQ+MASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQLMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
           GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV
Sbjct: 301 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQGTENDEKDG KDVSVPGAGFNFRGI+SSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGTENDEKDGVKDVSVPGAGFNFRGIISSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DI+EGSQNNVLSSSMQ                                  +F ++     
Sbjct: 421 DIEEGSQNNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTLFNLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLFNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ANKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVDGIDMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDGIDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILTSSIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTSSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPA 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGI QPSVSPSA  + ++TPPAPTISSPA
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGIMQPSVSPSAPHIVTSTPPAPTISSPA 822

Query: 925 SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           SVG+MASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 SVGVMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 822

BLAST of Sgr028084 vs. ExPASy TrEMBL
Match: A0A1S3CNJ3 (vacuolar protein sorting-associated protein 53 A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502491 PE=3 SV=1)

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 761/892 (85.31%), Postives = 780/892 (87.44%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGARGKE+GN IEEFGRED+NSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG ENDEK+G KD+SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPENDEKNGIKDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           IDSQLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPV GRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVFGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSP+DSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPVDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV-SPSAAPVASTTPPAPTISSP 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSV SPSA PV S+TPPAPT++SP
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSP 823

Query: 925 ASVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           ++VGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 STVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Sgr028084 vs. ExPASy TrEMBL
Match: A0A6J1IHL8 (vacuolar protein sorting-associated protein 53 A OS=Cucurbita maxima OX=3661 GN=LOC111477013 PE=3 SV=1)

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 757/891 (84.96%), Postives = 776/891 (87.09%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRR+RTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRMRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGA+GKEN NEIEEFGREDNNSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG E DEKDG K++SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPETDEKDGIKEMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KL ARLPKGG+GFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLLARLPKGGSGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           ID+QLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDTQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPA 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPS  P+AS  PPAPTI+SP 
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSVPPIASAAPPAPTITSP- 821

Query: 925 SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           +VG+MASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 TVGVMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Sgr028084 vs. ExPASy TrEMBL
Match: A0A6J1F767 (vacuolar protein sorting-associated protein 53 A OS=Cucurbita moschata OX=3662 GN=LOC111442766 PE=3 SV=1)

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 757/891 (84.96%), Postives = 776/891 (87.09%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
           AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA
Sbjct: 61  AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRR+RTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV
Sbjct: 241 LDKTERRYAWIKRRMRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
            TLLLALQRTLEFEDELAEKFGGGA+GKEN NEIEEFGREDNNSQNVSDIRKKYEKKLAV
Sbjct: 301 ATLLLALQRTLEFEDELAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAV 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
           HQG E DEKDG K++SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW
Sbjct: 361 HQGPETDEKDGIKEMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DIDEGSQ+NVLSSSMQ                                  +F ++     
Sbjct: 421 DIDEGSQSNVLSSSMQ----------------------------------LFLII----- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                         ++ LK    L KNQTL NLFKVFQRVLKAY
Sbjct: 481 ------------------------------KRSLKRCSALTKNQTLLNLFKVFQRVLKAY 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
           A KL ARLPKGG+GFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI
Sbjct: 541 ATKLLARLPKGGSGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 600

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
           ID+QLVDG+DMSEVQDEFSA+ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE
Sbjct: 601 IDTQLVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 660

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDK--------------------------MLL 804
           YVNGINMILT+SIPVLGRLLSPLYFQFFLDK                          MLL
Sbjct: 661 YVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLL 720

Query: 805 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 864
           DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE
Sbjct: 721 DTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPE 780

Query: 865 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPA 924
           GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPS  P+AS  PPAPTI+SP 
Sbjct: 781 GTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSVPPIASAAPPAPTITSP- 821

Query: 925 SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
           +VG+MASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 841 TVGVMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Sgr028084 vs. ExPASy TrEMBL
Match: A0A5D3DXS3 (Vacuolar protein sorting-associated protein 53 A isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G003100 PE=3 SV=1)

HSP 1 Score: 1359.7 bits (3518), Expect = 0.0e+00
Identity = 735/866 (84.87%), Postives = 754/866 (87.07%), Query Frame = 0

Query: 111 MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM 170
           MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM
Sbjct: 1   MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM 60

Query: 171 VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL 230
           VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL
Sbjct: 61  VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL 120

Query: 231 CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV 290
           CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV
Sbjct: 121 CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV 180

Query: 291 DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP 350
           DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP
Sbjct: 181 DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP 240

Query: 351 PSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVGTLLLALQRTLEFEDELAEKFGGGAR 410
           PSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV TLLLALQRTLEFEDELAEKFGGGAR
Sbjct: 241 PSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDELAEKFGGGAR 300

Query: 411 GKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGTENDEKDGTKDVSVPGAGFNFRG 470
           GKE+GN IEEFGRED+NSQNVSDIRKKYEKKLAVHQG ENDEK+G KD+SVPGAGFNFRG
Sbjct: 301 GKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMSVPGAGFNFRG 360

Query: 471 IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQNNVLSSSMQQLKGGGTIYY 530
           IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQ+NVLSSSMQ          
Sbjct: 361 IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ---------- 420

Query: 531 HYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMREVEEIKLAALLPENNLCIMPLPSKRI 590
                                   +F ++                               
Sbjct: 421 ------------------------LFLII------------------------------- 480

Query: 591 VSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAYAIKLFARLPKGGTGFVAAATGMDGQI 650
               ++ LK    L KNQTL NLFKVFQRVLKAYA KLFARLPKGGTGFVAAATGMDGQI
Sbjct: 481 ----KRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLFARLPKGGTGFVAAATGMDGQI 540

Query: 651 KTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGIDMSEVQDEFSALITKAL 710
           KTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDG+DMSEVQDEFSA+ITKAL
Sbjct: 541 KTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKAL 600

Query: 711 VTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTSSIPVLGRLLSPLYFQ 770
           VTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILT+SIPV GRLLSPLYFQ
Sbjct: 601 VTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVFGRLLSPLYFQ 660

Query: 771 FFLDK--------------------------MLLDTQAVKTILLDIPSLGRQTSGAASYS 830
           FFLDK                          MLLDTQAVKTILLDIPSLGRQTSGAASYS
Sbjct: 661 FFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASYS 720

Query: 831 KFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDD 890
           KFVSREMSKAEALLKVILSP+DSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDD
Sbjct: 721 KFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDD 780

Query: 891 FNKHGPGITQPSV-SPSAAPVASTTPPAPTISSPASVGLMASREDVLTRAAALGRGAATT 950
           FNKHGPGITQPSV SPSA PV S+TPPAPT++SP++VGLMASREDVLTRAAALGRGAATT
Sbjct: 781 FNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSPSTVGLMASREDVLTRAAALGRGAATT 797

BLAST of Sgr028084 vs. TAIR 10
Match: AT1G50500.1 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 653/897 (72.80%), Postives = 714/897 (79.60%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSSALEYINQMFPTEASL+GVEPLMQKI  EIRRVDA IL+AVRQQS+SGTKAKEDLA
Sbjct: 1   MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLA 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
            AT AVEEL  KI+EIK+KAEQSE MVQEICRDIKKLDFAKK+ITTTITALHRLTMLVSA
Sbjct: 61  DATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           VEQLQVMASKRQYKEAAAQLEAVNQLC+HFEAYRD PKITELREK  NIKQILKSHVFSD
Sbjct: 121 VEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTGKE EETNLLQ+LSD+C VVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK
Sbjct: 181 FSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LDKTERRYAWIKRRIRTNEEIWKIFP SWHVPYRLCIQFCK+TRKQ+E IL N+KEKP V
Sbjct: 241 LDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
             LLLALQ T+EFE EL +KFGGG   K+  ++IEE G  ++NSQN+S IRKKYEKK A 
Sbjct: 301 AILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAA 360

Query: 445 HQGTEND----EKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQ 504
            Q TE +    EK G KD+SV GAGFNFRG++SSCFEPHLT YIELEEKTLM++LEK+VQ
Sbjct: 361 SQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQ 420

Query: 505 EETWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLV 564
           EE+WD+++GSQNNVLSSS Q                                        
Sbjct: 421 EESWDVEDGSQNNVLSSSTQ---------------------------------------- 480

Query: 565 QRMREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRV 624
                                        + SN +K LK    L KNQTLFNLFKVFQRV
Sbjct: 481 -----------------------------LFSNIKKSLKRCNTLSKNQTLFNLFKVFQRV 540

Query: 625 LKAYAIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAES 684
           LKAYA KLF +LPKGGTG VAAATGMDGQIK S++DE+VICYIVNSAEYCHKTSGELAE+
Sbjct: 541 LKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAEN 600

Query: 685 VQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVG 744
           V +IID    DG+DMSEVQDEFSA+ITKALVTLV GLETKFD+EMA MTRVPW TLESVG
Sbjct: 601 VSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVG 660

Query: 745 DQSEYVNGINMILTSSIPVLGRLLSPLYFQFFLDK------------------------- 804
           DQS YVNGIN +L+ SIPVLG+LL+P+YFQFFLDK                         
Sbjct: 661 DQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQ 720

Query: 805 -MLLDTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRA 864
            MLLDTQAVK+ILL+IPSL RQTS AASYSKFVSREMS+AEALLKVILSPIDSVADTYRA
Sbjct: 721 QMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRA 780

Query: 865 LLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAP-- 924
           L PEGTPMEFQRILELKG KKADQQSILDDFNKHGPG TQ SV+ +A P    TPPAP  
Sbjct: 781 LFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVA-AAMPQPMPTPPAPPL 827

Query: 925 TISSPA-SVGLMASREDVLTRAAALGRGAATTGFKRFLALTEAAKDRKDGPFRKLFN 949
            I++PA + G +A+ EDVLTRAAALGRGAA+TGFK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 841 AITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 827

BLAST of Sgr028084 vs. TAIR 10
Match: AT1G50500.2 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 1175.6 bits (3040), Expect = 0.0e+00
Identity = 653/916 (71.29%), Postives = 714/916 (77.95%), Query Frame = 0

Query: 85  MDKSSALEYINQMFP-------------------TEASLSGVEPLMQKIHNEIRRVDAGI 144
           MDKSSALEYINQMFP                   TEASL+GVEPLMQKI  EIRRVDA I
Sbjct: 1   MDKSSALEYINQMFPTVSAYVTAFTMIFKCVFSFTEASLTGVEPLMQKIQGEIRRVDASI 60

Query: 145 LAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAK 204
           L+AVRQQS+SGTKAKEDLA AT AVEEL  KI+EIK+KAEQSE MVQEICRDIKKLDFAK
Sbjct: 61  LSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAK 120

Query: 205 KHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITE 264
           K+ITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLC+HFEAYRD PKITE
Sbjct: 121 KNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITE 180

Query: 265 LREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNF 324
           LREK  NIKQILKSHVFSDFSSLGTGKE EETNLLQ+LSD+C VVDALEPSVREELVNNF
Sbjct: 181 LREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNF 240

Query: 325 CSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCK 384
           CSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP SWHVPYRLCIQFCK
Sbjct: 241 CSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCK 300

Query: 385 KTRKQLEDILDNLKEKPDVGTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGRED 444
           +TRKQ+E IL N+KEKP V  LLLALQ T+EFE EL +KFGGG   K+  ++IEE G  +
Sbjct: 301 QTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWE 360

Query: 445 NNSQNVSDIRKKYEKKLAVHQGTEND----EKDGTKDVSVPGAGFNFRGIVSSCFEPHLT 504
           +NSQN+S IRKKYEKK A  Q TE +    EK G KD+SV GAGFNFRG++SSCFEPHLT
Sbjct: 361 DNSQNISKIRKKYEKKFAASQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLT 420

Query: 505 VYIELEEKTLMENLEKLVQEETWDIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQL 564
            YIELEEKTLM++LEK+VQEE+WD+++GSQNNVLSSS Q                     
Sbjct: 421 PYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQ--------------------- 480

Query: 565 MDFSGMSSYLGPSIFQVLVQRMREVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEM 624
                                                           + SN +K LK  
Sbjct: 481 ------------------------------------------------LFSNIKKSLKRC 540

Query: 625 QCLDKNQTLFNLFKVFQRVLKAYAIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVIC 684
             L KNQTLFNLFKVFQRVLKAYA KLF +LPKGGTG VAAATGMDGQIK S++DE+VIC
Sbjct: 541 NTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVIC 600

Query: 685 YIVNSAEYCHKTSGELAESVQKIIDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKF 744
           YIVNSAEYCHKTSGELAE+V +IID    DG+DMSEVQDEFSA+ITKALVTLV GLETKF
Sbjct: 601 YIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKF 660

Query: 745 DSEMAAMTRVPWGTLESVGDQSEYVNGINMILTSSIPVLGRLLSPLYFQFFLDK------ 804
           D+EMA MTRVPW TLESVGDQS YVNGIN +L+ SIPVLG+LL+P+YFQFFLDK      
Sbjct: 661 DTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLG 720

Query: 805 --------------------MLLDTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSKAE 864
                               MLLDTQAVK+ILL+IPSL RQTS AASYSKFVSREMS+AE
Sbjct: 721 PRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAE 780

Query: 865 ALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQP 924
           ALLKVILSPIDSVADTYRAL PEGTPMEFQRILELKG KKADQQSILDDFNKHGPG TQ 
Sbjct: 781 ALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQ 840

Query: 925 SVSPSAAPVASTTPPAP--TISSPA-SVGLMASREDVLTRAAALGRGAATTGFKRFLALT 949
           SV+ +A P    TPPAP   I++PA + G +A+ EDVLTRAAALGRGAA+TGFK+F+ALT
Sbjct: 841 SVA-AAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALT 846

BLAST of Sgr028084 vs. TAIR 10
Match: AT1G50970.1 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 1.8e-152
Identity = 384/873 (43.99%), Postives = 439/873 (50.29%), Query Frame = 0

Query: 85  MDKSSALEYINQMFPTEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLA 144
           MDKSS L++INQMFP EASLS VE  MQKI +EIRRVDA ILA V QQ +SGT+AKE+L 
Sbjct: 1   MDKSSGLDFINQMFPVEASLSCVESFMQKICDEIRRVDATILAVVSQQGNSGTRAKENLN 60

Query: 145 AATSAVEELMSKIREIKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSA 204
            A  A EEL  KI+EIK+KAEQ+E MVQ+IC DIKKLDFAKK+ITT +TAL RLTMLVSA
Sbjct: 61  DAICAAEELSHKIQEIKSKAEQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSA 120

Query: 205 VEQLQVMASKRQYKEAAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSD 264
           V+QLQVM SKRQYKEAA QLEA+N+LC+HF+AY D PKI ELREK KNIKQILK HVFSD
Sbjct: 121 VQQLQVMTSKRQYKEAATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQILKFHVFSD 180

Query: 265 FSSLGTGKEKEETNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAK 324
           FSSLGTG E EE  LL++LSD+C VVDALEPSVREEL+NNFCSRELTSYEQI+ GAEL  
Sbjct: 181 FSSLGTGTETEELFLLKKLSDSCLVVDALEPSVREELINNFCSRELTSYEQIYVGAELKT 240

Query: 325 LDKTERRYAWIKRRIRTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDV 384
           LD+ E  Y  +   IR N+  W IFP SWHVPYRLCIQ  +KTR Q+E IL NLKEK DV
Sbjct: 241 LDEIELIYNQLSCLIRKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSDV 300

Query: 385 GTLLLALQRTLEFEDELAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAV 444
             LLL L+RTLEFE EL  KFGGG      G++I   G   NNSQ               
Sbjct: 301 EKLLLELKRTLEFERELEMKFGGGG---SIGDDI--IGGGGNNSQK-------------- 360

Query: 445 HQGTENDEKDGTKDVSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETW 504
                                FNFRG++SSCFEPHLT+YIE EE  LM+ LEK+VQEETW
Sbjct: 361 ---------------------FNFRGMISSCFEPHLTIYIEKEEMELMQLLEKVVQEETW 420

Query: 505 DIDEGSQNNVLSSSMQQLKGGGTIYYHYSTFLDEEQLMDFSGMSSYLGPSIFQVLVQRMR 564
           DI+E                                                        
Sbjct: 421 DIEE-------------------------------------------------------- 480

Query: 565 EVEEIKLAALLPENNLCIMPLPSKRIVSNNQKELKEMQCLDKNQTLFNLFKVFQRVLKAY 624
                                              E+ C  +N                 
Sbjct: 481 -----------------------------------ELGCHSEN----------------- 540

Query: 625 AIKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKI 684
                                                                       
Sbjct: 541 ------------------------------------------------------------ 569

Query: 685 IDSQLVDGIDMSEVQDEFSALITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSE 744
                                                                     S 
Sbjct: 601 ----------------------------------------------------------SV 569

Query: 745 YVNGINMILTSSIPVLGRLLSPLYFQFFLDKMLLDTQAVKTILLDIPSLGRQ-------- 804
           Y+                             MLLD   +K ILL +PSL RQ        
Sbjct: 661 YL-----------------------------MLLDAHDMKMILLKVPSLARQPEASALLV 569

Query: 805 TSGAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKA 864
            +  ASY K V+ +M +AEA+LKVI SPI +V DTYRAL PE TPMEFQRIL LKG  KA
Sbjct: 721 KTATASYVKLVNHQMKRAEAVLKVIASPIVTVIDTYRALFPEETPMEFQRILVLKGLTKA 569

Query: 865 DQQSILDDFNKHGPGITQPSVSPSAAPVASTTPPAPTISSPASVGLMASREDVLTRAAAL 924
           +QQSILDDFN H   ITQ SV+ +  P A   P A T  +PA V   A+ E+VLTRAA+ 
Sbjct: 781 EQQSILDDFNNHSSRITQLSVA-AKTPEAHALPLALTNVAPA-VRFKANSEEVLTRAAS- 569

Query: 925 GRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 950
              AATT F +  ALT AAKDR   PFRKLFNP
Sbjct: 841 ---AATTSFMKLYALTGAAKDR---PFRKLFNP 569